^DATABASE = GeoMiame !Database_name = Gene Expression Omnibus (GEO) !Database_institute = NCBI NLM NIH !Database_web_link = http://www.ncbi.nlm.nih.gov/geo !Database_email = geo@ncbi.nlm.nih.gov ^SERIES = GSE115978 !Series_title = Single-cell RNA-seq of melanoma ecosystems reveals sources of T cells exclusion linked to immunotherapy clinical outcomes !Series_geo_accession = GSE115978 !Series_status = Public on Nov 01 2018 !Series_submission_date = Jun 18 2018 !Series_last_update_date = Mar 26 2019 !Series_pubmed_id = 30388455 !Series_summary = Immune checkpoint inhibitors (ICI) produce durable responses in some melanoma patients, but many patients derive no clinical benefit, and the molecular underpinnings of such resistance remain elusive. Here, we leveraged single-cell RNA-seq (scRNA-seq) from 31 melanoma tumors and novel computational methods to interrogate malignant cell states that promote immune evasion. We identified a resistance program expressed by malignant cells that is associated with T cell exclusion and immune evasion. The program is present prior to immunotherapy, characterizes cold niches, and predicts clinical responses to anti-PD-1 therapy in an independent cohort of 112 melanoma patients. CDK4/6-inhibition represses this program in individual malignant cells, induces senescence, and reduces melanoma tumor outgrowth in a mouse model when given in combination with immunotherapy. Our study provides a high-resolution landscape of ICI resistant cell states, identifies clinically predictive signatures, and forms a basis for the development of novel therapeutic strategies that could overcome immunotherapy resistance. !Series_overall_design = Individual cells were dissociated from fresh tumor resections, isolated immune and non-immune cells by FACS based on CD45 staining, and profiled with a modified full length SMART-Seq2 protocol. !Series_overall_design = !Series_overall_design = ** Submitter declares that the raw reads will be made available through dbGaP. ** !Series_type = Expression profiling by high throughput sequencing !Series_contributor = Livnat,,Jerby-Arnon !Series_contributor = Parin,,Shah !Series_contributor = Michael,S,Cuoco !Series_contributor = Christopher,,Rodman !Series_contributor = Mei,J,Su !Series_contributor = Johannes,C,Melms !Series_contributor = Rachel,,Leeson !Series_contributor = Abhay,,Kanodia !Series_contributor = Shaolin,,Mei !Series_contributor = Jia,R,Lin !Series_contributor = Shu,,Wang !Series_contributor = Bokang,,Rabasha !Series_contributor = David,,Liu !Series_contributor = Gao,,Zhang !Series_contributor = Claire,,Margolais !Series_contributor = Orr,,Ashenberg !Series_contributor = Patrick,A,Ott !Series_contributor = Elizabeth,I,Buchbinder !Series_contributor = Riz,,Haq !Series_contributor = F,S,Hodi !Series_contributor = G,M,Boland !Series_contributor = Ryan,J,Sullivan !Series_contributor = Dennie,T,Frederick !Series_contributor = Benchun,,Miao !Series_contributor = Tabea,,Moll !Series_contributor = Keith,T,Flaherty !Series_contributor = Meenhard,,Herlyn !Series_contributor = Russell,W,Jenkins !Series_contributor = Rohit,,Thummalapalli !Series_contributor = Monika,S,Kowalczyk !Series_contributor = Israel,,CaƱadas !Series_contributor = Bastian,,Schilling !Series_contributor = Adam,N,Cartwright !Series_contributor = Adrienne,M,Luoma !Series_contributor = Shruti,,Malu !Series_contributor = Patrick,,Hwu !Series_contributor = Chantale,,Bernatchez !Series_contributor = Marie,A,Forget !Series_contributor = David,A,Barbie !Series_contributor = Alex,K,Shalek !Series_contributor = Itay,,Tirosh !Series_contributor = Peter,K,Sorger !Series_contributor = Kai,,Wucherpfennig !Series_contributor = Eliezer,M,Van Allen !Series_contributor = Dirk,,Schadendorf !Series_contributor = Bruce,E,Johnson !Series_contributor = Asaf,,Rotem !Series_contributor = Orit,,Rozenblatt-Rosen !Series_contributor = Levi,A,Garraway !Series_contributor = Charles,H,Yoon !Series_contributor = Benjamin,,Izar !Series_contributor = Aviv,,Regev !Series_sample_id = GSM3197115 !Series_sample_id = GSM3197116 !Series_sample_id = GSM3197117 !Series_sample_id = GSM3197118 !Series_sample_id = GSM3197119 !Series_sample_id = GSM3197120 !Series_sample_id = GSM3197121 !Series_sample_id = GSM3197122 !Series_sample_id = GSM3197123 !Series_sample_id = GSM3197124 !Series_sample_id = GSM3197125 !Series_sample_id = GSM3197126 !Series_sample_id = GSM3197127 !Series_sample_id = GSM3197128 !Series_sample_id = GSM3197129 !Series_sample_id = GSM3197130 !Series_sample_id = GSM3197131 !Series_sample_id = GSM3197132 !Series_sample_id = GSM3197133 !Series_sample_id = GSM3197134 !Series_sample_id = GSM3197135 !Series_sample_id = GSM3197136 !Series_sample_id = GSM3197137 !Series_sample_id = GSM3197138 !Series_sample_id = GSM3197139 !Series_sample_id = GSM3197140 !Series_sample_id = GSM3197141 !Series_sample_id = GSM3197142 !Series_sample_id = GSM3197143 !Series_sample_id = GSM3197144 !Series_sample_id = GSM3197145 !Series_sample_id = GSM3197146 !Series_sample_id = GSM3197147 !Series_sample_id = GSM3197148 !Series_sample_id = GSM3197149 !Series_sample_id = GSM3197150 !Series_sample_id = GSM3197151 !Series_sample_id = GSM3197152 !Series_sample_id = GSM3197153 !Series_sample_id = GSM3197154 !Series_sample_id = GSM3197155 !Series_sample_id = GSM3197156 !Series_sample_id = GSM3197157 !Series_sample_id = GSM3197158 !Series_sample_id = GSM3197159 !Series_sample_id = GSM3197160 !Series_sample_id = GSM3197161 !Series_sample_id = GSM3197162 !Series_sample_id = GSM3197163 !Series_sample_id = GSM3197164 !Series_sample_id = GSM3197165 !Series_sample_id = GSM3197166 !Series_sample_id = GSM3197167 !Series_sample_id = GSM3197168 !Series_sample_id = GSM3197169 !Series_sample_id = GSM3197170 !Series_sample_id = GSM3197171 !Series_sample_id = GSM3197172 !Series_sample_id = GSM3197173 !Series_sample_id = GSM3197174 !Series_sample_id = GSM3197175 !Series_sample_id = GSM3197176 !Series_sample_id = GSM3197177 !Series_sample_id = GSM3197178 !Series_sample_id = GSM3197179 !Series_sample_id = GSM3197180 !Series_sample_id = GSM3197181 !Series_sample_id = GSM3197182 !Series_sample_id = GSM3197183 !Series_sample_id = GSM3197184 !Series_sample_id = GSM3197185 !Series_sample_id = GSM3197186 !Series_sample_id = GSM3197187 !Series_sample_id = GSM3197188 !Series_sample_id = GSM3197189 !Series_sample_id = GSM3197190 !Series_sample_id = GSM3197191 !Series_sample_id = GSM3197192 !Series_sample_id = GSM3197193 !Series_sample_id = GSM3197194 !Series_sample_id = GSM3197195 !Series_sample_id = GSM3197196 !Series_sample_id = GSM3197197 !Series_sample_id = GSM3197198 !Series_sample_id = GSM3197199 !Series_sample_id = GSM3197200 !Series_sample_id = GSM3197201 !Series_sample_id = GSM3197202 !Series_sample_id = GSM3197203 !Series_sample_id = GSM3197204 !Series_sample_id = GSM3197205 !Series_sample_id = GSM3197206 !Series_sample_id = GSM3197207 !Series_sample_id = GSM3197208 !Series_sample_id = GSM3197209 !Series_sample_id = GSM3197210 !Series_sample_id = GSM3197211 !Series_sample_id = GSM3197212 !Series_sample_id = GSM3197213 !Series_sample_id = GSM3197214 !Series_sample_id = GSM3197215 !Series_sample_id = GSM3197216 !Series_sample_id = GSM3197217 !Series_sample_id = GSM3197218 !Series_sample_id = GSM3197219 !Series_sample_id = GSM3197220 !Series_sample_id = GSM3197221 !Series_sample_id = GSM3197222 !Series_sample_id = GSM3197223 !Series_sample_id = GSM3197224 !Series_sample_id = GSM3197225 !Series_sample_id = GSM3197226 !Series_sample_id = GSM3197227 !Series_sample_id = GSM3197228 !Series_sample_id = GSM3197229 !Series_sample_id = GSM3197230 !Series_sample_id = GSM3197231 !Series_sample_id = GSM3197232 !Series_sample_id = GSM3197233 !Series_sample_id = GSM3197234 !Series_sample_id = GSM3197235 !Series_sample_id = GSM3197236 !Series_sample_id = GSM3197237 !Series_sample_id = GSM3197238 !Series_sample_id = GSM3197239 !Series_sample_id = GSM3197240 !Series_sample_id = GSM3197241 !Series_sample_id = GSM3197242 !Series_sample_id = GSM3197243 !Series_sample_id = GSM3197244 !Series_sample_id = GSM3197245 !Series_sample_id = GSM3197246 !Series_sample_id = GSM3197247 !Series_sample_id = GSM3197248 !Series_sample_id = GSM3197249 !Series_sample_id = GSM3197250 !Series_sample_id = GSM3197251 !Series_sample_id = GSM3197252 !Series_sample_id = GSM3197253 !Series_sample_id = GSM3197254 !Series_sample_id = GSM3197255 !Series_sample_id = GSM3197256 !Series_sample_id = GSM3197257 !Series_sample_id = GSM3197258 !Series_sample_id = GSM3197259 !Series_sample_id = GSM3197260 !Series_sample_id = GSM3197261 !Series_sample_id = GSM3197262 !Series_sample_id = GSM3197263 !Series_sample_id = GSM3197264 !Series_sample_id = GSM3197265 !Series_sample_id = GSM3197266 !Series_sample_id = GSM3197267 !Series_sample_id = GSM3197268 !Series_sample_id = GSM3197269 !Series_sample_id = GSM3197270 !Series_sample_id = GSM3197271 !Series_sample_id = GSM3197272 !Series_sample_id = GSM3197273 !Series_sample_id = GSM3197274 !Series_sample_id = GSM3197275 !Series_sample_id = GSM3197276 !Series_sample_id = GSM3197277 !Series_sample_id = GSM3197278 !Series_sample_id = GSM3197279 !Series_sample_id = GSM3197280 !Series_sample_id = GSM3197281 !Series_sample_id = GSM3197282 !Series_sample_id = GSM3197283 !Series_sample_id = GSM3197284 !Series_sample_id = GSM3197285 !Series_sample_id = GSM3197286 !Series_sample_id = GSM3197287 !Series_sample_id = GSM3197288 !Series_sample_id = GSM3197289 !Series_sample_id = GSM3197290 !Series_sample_id = GSM3197291 !Series_sample_id = GSM3197292 !Series_sample_id = GSM3197293 !Series_sample_id = GSM3197294 !Series_sample_id = GSM3197295 !Series_sample_id = GSM3197296 !Series_sample_id = GSM3197297 !Series_sample_id = GSM3197298 !Series_sample_id = GSM3197299 !Series_sample_id = GSM3197300 !Series_sample_id = GSM3197301 !Series_sample_id = GSM3197302 !Series_sample_id = GSM3197303 !Series_sample_id = GSM3197304 !Series_sample_id = GSM3197305 !Series_sample_id = GSM3197306 !Series_sample_id = GSM3197307 !Series_sample_id = GSM3197308 !Series_sample_id = GSM3197309 !Series_sample_id = GSM3197310 !Series_sample_id = GSM3197311 !Series_sample_id = GSM3197312 !Series_sample_id = GSM3197313 !Series_sample_id = GSM3197314 !Series_sample_id = GSM3197315 !Series_sample_id = GSM3197316 !Series_sample_id = GSM3197317 !Series_sample_id = GSM3197318 !Series_sample_id = GSM3197319 !Series_sample_id = GSM3197320 !Series_sample_id = GSM3197321 !Series_sample_id = GSM3197322 !Series_sample_id = GSM3197323 !Series_sample_id = GSM3197324 !Series_sample_id = GSM3197325 !Series_sample_id = GSM3197326 !Series_sample_id = GSM3197327 !Series_sample_id = GSM3197328 !Series_sample_id = GSM3197329 !Series_sample_id = GSM3197330 !Series_sample_id = GSM3197331 !Series_sample_id = GSM3197332 !Series_sample_id = GSM3197333 !Series_sample_id = GSM3197334 !Series_sample_id = GSM3197335 !Series_sample_id = GSM3197336 !Series_sample_id = GSM3197337 !Series_sample_id = GSM3197338 !Series_sample_id = GSM3197339 !Series_sample_id = GSM3197340 !Series_sample_id = GSM3197341 !Series_sample_id = GSM3197342 !Series_sample_id = GSM3197343 !Series_sample_id = GSM3197344 !Series_sample_id = GSM3197345 !Series_sample_id = GSM3197346 !Series_sample_id = GSM3197347 !Series_sample_id = GSM3197348 !Series_sample_id = GSM3197349 !Series_sample_id = GSM3197350 !Series_sample_id = GSM3197351 !Series_sample_id = GSM3197352 !Series_sample_id = GSM3197353 !Series_sample_id = GSM3197354 !Series_sample_id = GSM3197355 !Series_sample_id = GSM3197356 !Series_sample_id = GSM3197357 !Series_sample_id = GSM3197358 !Series_sample_id = GSM3197359 !Series_sample_id = GSM3197360 !Series_sample_id = GSM3197361 !Series_sample_id = GSM3197362 !Series_sample_id = GSM3197363 !Series_sample_id = GSM3197364 !Series_sample_id = GSM3197365 !Series_sample_id = GSM3197366 !Series_sample_id = GSM3197367 !Series_sample_id = GSM3197368 !Series_sample_id = GSM3197369 !Series_sample_id = GSM3197370 !Series_sample_id = GSM3197371 !Series_sample_id = GSM3197372 !Series_sample_id = GSM3197373 !Series_sample_id = GSM3197374 !Series_sample_id = GSM3197375 !Series_sample_id = GSM3197376 !Series_sample_id = GSM3197377 !Series_sample_id = GSM3197378 !Series_sample_id = GSM3197379 !Series_sample_id = GSM3197380 !Series_sample_id = GSM3197381 !Series_sample_id = GSM3197382 !Series_sample_id = GSM3197383 !Series_sample_id = GSM3197384 !Series_sample_id = GSM3197385 !Series_sample_id = GSM3197386 !Series_sample_id = GSM3197387 !Series_sample_id = GSM3197388 !Series_sample_id = GSM3197389 !Series_sample_id = GSM3197390 !Series_sample_id = GSM3197391 !Series_sample_id = GSM3197392 !Series_sample_id = GSM3197393 !Series_sample_id = GSM3197394 !Series_sample_id = GSM3197395 !Series_sample_id = GSM3197396 !Series_sample_id = GSM3197397 !Series_sample_id = GSM3197398 !Series_sample_id = GSM3197399 !Series_sample_id = GSM3197400 !Series_sample_id = GSM3197401 !Series_sample_id = GSM3197402 !Series_sample_id = GSM3197403 !Series_sample_id = GSM3197404 !Series_sample_id = GSM3197405 !Series_sample_id = GSM3197406 !Series_sample_id = GSM3197407 !Series_sample_id = GSM3197408 !Series_sample_id = GSM3197409 !Series_sample_id = GSM3197410 !Series_sample_id = GSM3197411 !Series_sample_id = GSM3197412 !Series_sample_id = GSM3197413 !Series_sample_id = GSM3197414 !Series_sample_id = GSM3197415 !Series_sample_id = GSM3197416 !Series_sample_id = GSM3197417 !Series_sample_id = GSM3197418 !Series_sample_id = GSM3197419 !Series_sample_id = GSM3197420 !Series_sample_id = GSM3197421 !Series_sample_id = GSM3197422 !Series_sample_id = GSM3197423 !Series_sample_id = GSM3197424 !Series_sample_id = GSM3197425 !Series_sample_id = GSM3197426 !Series_sample_id = GSM3197427 !Series_sample_id = GSM3197428 !Series_sample_id = GSM3197429 !Series_sample_id = GSM3197430 !Series_sample_id = GSM3197431 !Series_sample_id = GSM3197432 !Series_sample_id = GSM3197433 !Series_sample_id = GSM3197434 !Series_sample_id = GSM3197435 !Series_sample_id = GSM3197436 !Series_sample_id = GSM3197437 !Series_sample_id = GSM3197438 !Series_sample_id = GSM3197439 !Series_sample_id = GSM3197440 !Series_sample_id = GSM3197441 !Series_sample_id = GSM3197442 !Series_sample_id = GSM3197443 !Series_sample_id = GSM3197444 !Series_sample_id = GSM3197445 !Series_sample_id = GSM3197446 !Series_sample_id = GSM3197447 !Series_sample_id = GSM3197448 !Series_sample_id = GSM3197449 !Series_sample_id = GSM3197450 !Series_sample_id = GSM3197451 !Series_sample_id = GSM3197452 !Series_sample_id = GSM3197453 !Series_sample_id = GSM3197454 !Series_sample_id = GSM3197455 !Series_sample_id = GSM3197456 !Series_sample_id = GSM3197457 !Series_sample_id = GSM3197458 !Series_sample_id = GSM3197459 !Series_sample_id = GSM3197460 !Series_sample_id = GSM3197461 !Series_sample_id = GSM3197462 !Series_sample_id = GSM3197463 !Series_sample_id = GSM3197464 !Series_sample_id = GSM3197465 !Series_sample_id = GSM3197466 !Series_sample_id = GSM3197467 !Series_sample_id = GSM3197468 !Series_sample_id = GSM3197469 !Series_sample_id = GSM3197470 !Series_sample_id = GSM3197471 !Series_sample_id = GSM3197472 !Series_sample_id = GSM3197473 !Series_sample_id = GSM3197474 !Series_sample_id = GSM3197475 !Series_sample_id = GSM3197476 !Series_sample_id = GSM3197477 !Series_sample_id = GSM3197478 !Series_sample_id = GSM3197479 !Series_sample_id = GSM3197480 !Series_sample_id = GSM3197481 !Series_sample_id = GSM3197482 !Series_sample_id = GSM3197483 !Series_sample_id = GSM3197484 !Series_sample_id = GSM3197485 !Series_sample_id = GSM3197486 !Series_sample_id = GSM3197487 !Series_sample_id = GSM3197488 !Series_sample_id = GSM3197489 !Series_sample_id = GSM3197490 !Series_sample_id = GSM3197491 !Series_sample_id = GSM3197492 !Series_sample_id = GSM3197493 !Series_sample_id = GSM3197494 !Series_sample_id = GSM3197495 !Series_sample_id = GSM3197496 !Series_sample_id = GSM3197497 !Series_sample_id = GSM3197498 !Series_sample_id = GSM3197499 !Series_sample_id = GSM3197500 !Series_sample_id = GSM3197501 !Series_sample_id = GSM3197502 !Series_sample_id = GSM3197503 !Series_sample_id = GSM3197504 !Series_sample_id = GSM3197505 !Series_sample_id = GSM3197506 !Series_sample_id = GSM3197507 !Series_sample_id = GSM3197508 !Series_sample_id = GSM3197509 !Series_sample_id = GSM3197510 !Series_sample_id = GSM3197511 !Series_sample_id = GSM3197512 !Series_sample_id = GSM3197513 !Series_sample_id = GSM3197514 !Series_sample_id = GSM3197515 !Series_sample_id = GSM3197516 !Series_sample_id = GSM3197517 !Series_sample_id = GSM3197518 !Series_sample_id = GSM3197519 !Series_sample_id = GSM3197520 !Series_sample_id = GSM3197521 !Series_sample_id = GSM3197522 !Series_sample_id = GSM3197523 !Series_sample_id = GSM3197524 !Series_sample_id = GSM3197525 !Series_sample_id = GSM3197526 !Series_sample_id = GSM3197527 !Series_sample_id = GSM3197528 !Series_sample_id = GSM3197529 !Series_sample_id = GSM3197530 !Series_sample_id = GSM3197531 !Series_sample_id = GSM3197532 !Series_sample_id = GSM3197533 !Series_sample_id = GSM3197534 !Series_sample_id = GSM3197535 !Series_sample_id = GSM3197536 !Series_sample_id = GSM3197537 !Series_sample_id = GSM3197538 !Series_sample_id = GSM3197539 !Series_sample_id = GSM3197540 !Series_sample_id = GSM3197541 !Series_sample_id = GSM3197542 !Series_sample_id = GSM3197543 !Series_sample_id = GSM3197544 !Series_sample_id = GSM3197545 !Series_sample_id = GSM3197546 !Series_sample_id = GSM3197547 !Series_sample_id = GSM3197548 !Series_sample_id = GSM3197549 !Series_sample_id = GSM3197550 !Series_sample_id = GSM3197551 !Series_sample_id = GSM3197552 !Series_sample_id = GSM3197553 !Series_sample_id = GSM3197554 !Series_sample_id = GSM3197555 !Series_sample_id = GSM3197556 !Series_sample_id = GSM3197557 !Series_sample_id = GSM3197558 !Series_sample_id = GSM3197559 !Series_sample_id = GSM3197560 !Series_sample_id = GSM3197561 !Series_sample_id = GSM3197562 !Series_sample_id = GSM3197563 !Series_sample_id = GSM3197564 !Series_sample_id = GSM3197565 !Series_sample_id = GSM3197566 !Series_sample_id = GSM3197567 !Series_sample_id = GSM3197568 !Series_sample_id = GSM3197569 !Series_sample_id = GSM3197570 !Series_sample_id = GSM3197571 !Series_sample_id = GSM3197572 !Series_sample_id = GSM3197573 !Series_sample_id = GSM3197574 !Series_sample_id = GSM3197575 !Series_sample_id = GSM3197576 !Series_sample_id = GSM3197577 !Series_sample_id = GSM3197578 !Series_sample_id = GSM3197579 !Series_sample_id = GSM3197580 !Series_sample_id = GSM3197581 !Series_sample_id = GSM3197582 !Series_sample_id = GSM3197583 !Series_sample_id = GSM3197584 !Series_sample_id = GSM3197585 !Series_sample_id = GSM3197586 !Series_sample_id = GSM3197587 !Series_sample_id = GSM3197588 !Series_sample_id = GSM3197589 !Series_sample_id = GSM3197590 !Series_sample_id = GSM3197591 !Series_sample_id = GSM3197592 !Series_sample_id = GSM3197593 !Series_sample_id = GSM3197594 !Series_sample_id = GSM3197595 !Series_sample_id = GSM3197596 !Series_sample_id = GSM3197597 !Series_sample_id = GSM3197598 !Series_sample_id = GSM3197599 !Series_sample_id = GSM3197600 !Series_sample_id = GSM3197601 !Series_sample_id = GSM3197602 !Series_sample_id = GSM3197603 !Series_sample_id = GSM3197604 !Series_sample_id = GSM3197605 !Series_sample_id = GSM3197606 !Series_sample_id = GSM3197607 !Series_sample_id = GSM3197608 !Series_sample_id = GSM3197609 !Series_sample_id = GSM3197610 !Series_sample_id = GSM3197611 !Series_sample_id = GSM3197612 !Series_sample_id = GSM3197613 !Series_sample_id = GSM3197614 !Series_sample_id = GSM3197615 !Series_sample_id = GSM3197616 !Series_sample_id = GSM3197617 !Series_sample_id = GSM3197618 !Series_sample_id = GSM3197619 !Series_sample_id = GSM3197620 !Series_sample_id = GSM3197621 !Series_sample_id = GSM3197622 !Series_sample_id = GSM3197623 !Series_sample_id = GSM3197624 !Series_sample_id = GSM3197625 !Series_sample_id = GSM3197626 !Series_sample_id = GSM3197627 !Series_sample_id = GSM3197628 !Series_sample_id = GSM3197629 !Series_sample_id = GSM3197630 !Series_sample_id = GSM3197631 !Series_sample_id = GSM3197632 !Series_sample_id = GSM3197633 !Series_sample_id = GSM3197634 !Series_sample_id = GSM3197635 !Series_sample_id = GSM3197636 !Series_sample_id = GSM3197637 !Series_sample_id = GSM3197638 !Series_sample_id = GSM3197639 !Series_sample_id = GSM3197640 !Series_sample_id = GSM3197641 !Series_sample_id = GSM3197642 !Series_sample_id = GSM3197643 !Series_sample_id = GSM3197644 !Series_sample_id = GSM3197645 !Series_sample_id = GSM3197646 !Series_sample_id = GSM3197647 !Series_sample_id = GSM3197648 !Series_sample_id = GSM3197649 !Series_sample_id = GSM3197650 !Series_sample_id = GSM3197651 !Series_sample_id = GSM3197652 !Series_sample_id = GSM3197653 !Series_sample_id = GSM3197654 !Series_sample_id = GSM3197655 !Series_sample_id = GSM3197656 !Series_sample_id = GSM3197657 !Series_sample_id = GSM3197658 !Series_sample_id = GSM3197659 !Series_sample_id = GSM3197660 !Series_sample_id = GSM3197661 !Series_sample_id = GSM3197662 !Series_sample_id = GSM3197663 !Series_sample_id = GSM3197664 !Series_sample_id = GSM3197665 !Series_sample_id = GSM3197666 !Series_sample_id = GSM3197667 !Series_sample_id = GSM3197668 !Series_sample_id = GSM3197669 !Series_sample_id = GSM3197670 !Series_sample_id = GSM3197671 !Series_sample_id = GSM3197672 !Series_sample_id = GSM3197673 !Series_sample_id = GSM3197674 !Series_sample_id = GSM3197675 !Series_sample_id = GSM3197676 !Series_sample_id = GSM3197677 !Series_sample_id = GSM3197678 !Series_sample_id = GSM3197679 !Series_sample_id = GSM3197680 !Series_sample_id = GSM3197681 !Series_sample_id = GSM3197682 !Series_sample_id = GSM3197683 !Series_sample_id = GSM3197684 !Series_sample_id = GSM3197685 !Series_sample_id = GSM3197686 !Series_sample_id = GSM3197687 !Series_sample_id = GSM3197688 !Series_sample_id = GSM3197689 !Series_sample_id = GSM3197690 !Series_sample_id = GSM3197691 !Series_sample_id = GSM3197692 !Series_sample_id = GSM3197693 !Series_sample_id = GSM3197694 !Series_sample_id = GSM3197695 !Series_sample_id = GSM3197696 !Series_sample_id = GSM3197697 !Series_sample_id = GSM3197698 !Series_sample_id = GSM3197699 !Series_sample_id = GSM3197700 !Series_sample_id = GSM3197701 !Series_sample_id = GSM3197702 !Series_sample_id = GSM3197703 !Series_sample_id = GSM3197704 !Series_sample_id = GSM3197705 !Series_sample_id = GSM3197706 !Series_sample_id = GSM3197707 !Series_sample_id = GSM3197708 !Series_sample_id = GSM3197709 !Series_sample_id = GSM3197710 !Series_sample_id = GSM3197711 !Series_sample_id = GSM3197712 !Series_sample_id = GSM3197713 !Series_sample_id = GSM3197714 !Series_sample_id = GSM3197715 !Series_sample_id = GSM3197716 !Series_sample_id = GSM3197717 !Series_sample_id = GSM3197718 !Series_sample_id = GSM3197719 !Series_sample_id = GSM3197720 !Series_sample_id = GSM3197721 !Series_sample_id = GSM3197722 !Series_sample_id = GSM3197723 !Series_sample_id = GSM3197724 !Series_sample_id = GSM3197725 !Series_sample_id = GSM3197726 !Series_sample_id = GSM3197727 !Series_sample_id = GSM3197728 !Series_sample_id = GSM3197729 !Series_sample_id = GSM3197730 !Series_sample_id = GSM3197731 !Series_sample_id = GSM3197732 !Series_sample_id = GSM3197733 !Series_sample_id = GSM3197734 !Series_sample_id = GSM3197735 !Series_sample_id = GSM3197736 !Series_sample_id = GSM3197737 !Series_sample_id = GSM3197738 !Series_sample_id = GSM3197739 !Series_sample_id = GSM3197740 !Series_sample_id = GSM3197741 !Series_sample_id = GSM3197742 !Series_sample_id = GSM3197743 !Series_sample_id = GSM3197744 !Series_sample_id = GSM3197745 !Series_sample_id = GSM3197746 !Series_sample_id = GSM3197747 !Series_sample_id = GSM3197748 !Series_sample_id = GSM3197749 !Series_sample_id = GSM3197750 !Series_sample_id = GSM3197751 !Series_sample_id = GSM3197752 !Series_sample_id = GSM3197753 !Series_sample_id = GSM3197754 !Series_sample_id = GSM3197755 !Series_sample_id = GSM3197756 !Series_sample_id = GSM3197757 !Series_sample_id = GSM3197758 !Series_sample_id = GSM3197759 !Series_sample_id = GSM3197760 !Series_sample_id = GSM3197761 !Series_sample_id = GSM3197762 !Series_sample_id = GSM3197763 !Series_sample_id = GSM3197764 !Series_sample_id = GSM3197765 !Series_sample_id = GSM3197766 !Series_sample_id = GSM3197767 !Series_sample_id = GSM3197768 !Series_sample_id = GSM3197769 !Series_sample_id = GSM3197770 !Series_sample_id = GSM3197771 !Series_sample_id = GSM3197772 !Series_sample_id = GSM3197773 !Series_sample_id = GSM3197774 !Series_sample_id = GSM3197775 !Series_sample_id = GSM3197776 !Series_sample_id = GSM3197777 !Series_sample_id = GSM3197778 !Series_sample_id = GSM3197779 !Series_sample_id = GSM3197780 !Series_sample_id = GSM3197781 !Series_sample_id = GSM3197782 !Series_sample_id = GSM3197783 !Series_sample_id = GSM3197784 !Series_sample_id = GSM3197785 !Series_sample_id = GSM3197786 !Series_sample_id = GSM3197787 !Series_sample_id = GSM3197788 !Series_sample_id = GSM3197789 !Series_sample_id = GSM3197790 !Series_sample_id = GSM3197791 !Series_sample_id = GSM3197792 !Series_sample_id = GSM3197793 !Series_sample_id = GSM3197794 !Series_sample_id = GSM3197795 !Series_sample_id = GSM3197796 !Series_sample_id = GSM3197797 !Series_sample_id = GSM3197798 !Series_sample_id = GSM3197799 !Series_sample_id = GSM3197800 !Series_sample_id = GSM3197801 !Series_sample_id = GSM3197802 !Series_sample_id = GSM3197803 !Series_sample_id = GSM3197804 !Series_sample_id = GSM3197805 !Series_sample_id = GSM3197806 !Series_sample_id = GSM3197807 !Series_sample_id = GSM3197808 !Series_sample_id = GSM3197809 !Series_sample_id = GSM3197810 !Series_sample_id = GSM3197811 !Series_sample_id = GSM3197812 !Series_sample_id = GSM3197813 !Series_sample_id = GSM3197814 !Series_sample_id = GSM3197815 !Series_sample_id = GSM3197816 !Series_sample_id = GSM3197817 !Series_sample_id = GSM3197818 !Series_sample_id = GSM3197819 !Series_sample_id = GSM3197820 !Series_sample_id = GSM3197821 !Series_sample_id = GSM3197822 !Series_sample_id = GSM3197823 !Series_sample_id = GSM3197824 !Series_sample_id = GSM3197825 !Series_sample_id = GSM3197826 !Series_sample_id = GSM3197827 !Series_sample_id = GSM3197828 !Series_sample_id = GSM3197829 !Series_sample_id = GSM3197830 !Series_sample_id = GSM3197831 !Series_sample_id = GSM3197832 !Series_sample_id = GSM3197833 !Series_sample_id = GSM3197834 !Series_sample_id = GSM3197835 !Series_sample_id = GSM3197836 !Series_sample_id = GSM3197837 !Series_sample_id = GSM3197838 !Series_sample_id = GSM3197839 !Series_sample_id = GSM3197840 !Series_sample_id = GSM3197841 !Series_sample_id = GSM3197842 !Series_sample_id = GSM3197843 !Series_sample_id = GSM3197844 !Series_sample_id = GSM3197845 !Series_sample_id = GSM3197846 !Series_sample_id = GSM3197847 !Series_sample_id = GSM3197848 !Series_sample_id = GSM3197849 !Series_sample_id = GSM3197850 !Series_sample_id = GSM3197851 !Series_sample_id = GSM3197852 !Series_sample_id = GSM3197853 !Series_sample_id = GSM3197854 !Series_sample_id = GSM3197855 !Series_sample_id = GSM3197856 !Series_sample_id = GSM3197857 !Series_sample_id = GSM3197858 !Series_sample_id = GSM3197859 !Series_sample_id = GSM3197860 !Series_sample_id = GSM3197861 !Series_sample_id = GSM3197862 !Series_sample_id = GSM3197863 !Series_sample_id = GSM3197864 !Series_sample_id = GSM3197865 !Series_sample_id = GSM3197866 !Series_sample_id = GSM3197867 !Series_sample_id = GSM3197868 !Series_sample_id = GSM3197869 !Series_sample_id = GSM3197870 !Series_sample_id = GSM3197871 !Series_sample_id = GSM3197872 !Series_sample_id = GSM3197873 !Series_sample_id = GSM3197874 !Series_sample_id = GSM3197875 !Series_sample_id = GSM3197876 !Series_sample_id = GSM3197877 !Series_sample_id = GSM3197878 !Series_sample_id = GSM3197879 !Series_sample_id = GSM3197880 !Series_sample_id = GSM3197881 !Series_sample_id = GSM3197882 !Series_sample_id = GSM3197883 !Series_sample_id = GSM3197884 !Series_sample_id = GSM3197885 !Series_sample_id = GSM3197886 !Series_sample_id = GSM3197887 !Series_sample_id = GSM3197888 !Series_sample_id = GSM3197889 !Series_sample_id = GSM3197890 !Series_sample_id = GSM3197891 !Series_sample_id = GSM3197892 !Series_sample_id = GSM3197893 !Series_sample_id = GSM3197894 !Series_sample_id = GSM3197895 !Series_sample_id = GSM3197896 !Series_sample_id = GSM3197897 !Series_sample_id = GSM3197898 !Series_sample_id = GSM3197899 !Series_sample_id = GSM3197900 !Series_sample_id = GSM3197901 !Series_sample_id = GSM3197902 !Series_sample_id = GSM3197903 !Series_sample_id = GSM3197904 !Series_sample_id = GSM3197905 !Series_sample_id = GSM3197906 !Series_sample_id = GSM3197907 !Series_sample_id = GSM3197908 !Series_sample_id = GSM3197909 !Series_sample_id = GSM3197910 !Series_sample_id = GSM3197911 !Series_sample_id = GSM3197912 !Series_sample_id = GSM3197913 !Series_sample_id = GSM3197914 !Series_sample_id = GSM3197915 !Series_sample_id = GSM3197916 !Series_sample_id = GSM3197917 !Series_sample_id = GSM3197918 !Series_sample_id = GSM3197919 !Series_sample_id = GSM3197920 !Series_sample_id = GSM3197921 !Series_sample_id = GSM3197922 !Series_sample_id = GSM3197923 !Series_sample_id = GSM3197924 !Series_sample_id = GSM3197925 !Series_sample_id = GSM3197926 !Series_sample_id = GSM3197927 !Series_sample_id = GSM3197928 !Series_sample_id = GSM3197929 !Series_sample_id = GSM3197930 !Series_sample_id = GSM3197931 !Series_sample_id = GSM3197932 !Series_sample_id = GSM3197933 !Series_sample_id = GSM3197934 !Series_sample_id = GSM3197935 !Series_sample_id = GSM3197936 !Series_sample_id = GSM3197937 !Series_sample_id = GSM3197938 !Series_sample_id = GSM3197939 !Series_sample_id = GSM3197940 !Series_sample_id = GSM3197941 !Series_sample_id = GSM3197942 !Series_sample_id = GSM3197943 !Series_sample_id = GSM3197944 !Series_sample_id = GSM3197945 !Series_sample_id = GSM3197946 !Series_sample_id = GSM3197947 !Series_sample_id = GSM3197948 !Series_sample_id = GSM3197949 !Series_sample_id = GSM3197950 !Series_sample_id = GSM3197951 !Series_sample_id = GSM3197952 !Series_sample_id = GSM3197953 !Series_sample_id = GSM3197954 !Series_sample_id = GSM3197955 !Series_sample_id = GSM3197956 !Series_sample_id = GSM3197957 !Series_sample_id = GSM3197958 !Series_sample_id = GSM3197959 !Series_sample_id = GSM3197960 !Series_sample_id = GSM3197961 !Series_sample_id = GSM3197962 !Series_sample_id = GSM3197963 !Series_sample_id = GSM3197964 !Series_sample_id = GSM3197965 !Series_sample_id = GSM3197966 !Series_sample_id = GSM3197967 !Series_sample_id = GSM3197968 !Series_sample_id = GSM3197969 !Series_sample_id = GSM3197970 !Series_sample_id = GSM3197971 !Series_sample_id = GSM3197972 !Series_sample_id = GSM3197973 !Series_sample_id = GSM3197974 !Series_sample_id = GSM3197975 !Series_sample_id = GSM3197976 !Series_sample_id = GSM3197977 !Series_sample_id = GSM3197978 !Series_sample_id = GSM3197979 !Series_sample_id = GSM3197980 !Series_sample_id = GSM3197981 !Series_sample_id = GSM3197982 !Series_sample_id = GSM3197983 !Series_sample_id = GSM3197984 !Series_sample_id = GSM3197985 !Series_sample_id = GSM3197986 !Series_sample_id = GSM3197987 !Series_sample_id = GSM3197988 !Series_sample_id = GSM3197989 !Series_sample_id = GSM3197990 !Series_sample_id = GSM3197991 !Series_sample_id = GSM3197992 !Series_sample_id = GSM3197993 !Series_sample_id = GSM3197994 !Series_sample_id = GSM3197995 !Series_sample_id = GSM3197996 !Series_sample_id = GSM3197997 !Series_sample_id = GSM3197998 !Series_sample_id = GSM3197999 !Series_sample_id = GSM3198000 !Series_sample_id = GSM3198001 !Series_sample_id = GSM3198002 !Series_sample_id = GSM3198003 !Series_sample_id = GSM3198004 !Series_sample_id = GSM3198005 !Series_sample_id = GSM3198006 !Series_sample_id = GSM3198007 !Series_sample_id = GSM3198008 !Series_sample_id = GSM3198009 !Series_sample_id = GSM3198010 !Series_sample_id = GSM3198011 !Series_sample_id = GSM3198012 !Series_sample_id = GSM3198013 !Series_sample_id = GSM3198014 !Series_sample_id = GSM3198015 !Series_sample_id = GSM3198016 !Series_sample_id = GSM3198017 !Series_sample_id = GSM3198018 !Series_sample_id = GSM3198019 !Series_sample_id = GSM3198020 !Series_sample_id = GSM3198021 !Series_sample_id = GSM3198022 !Series_sample_id = GSM3198023 !Series_sample_id = GSM3198024 !Series_sample_id = GSM3198025 !Series_sample_id = GSM3198026 !Series_sample_id = GSM3198027 !Series_sample_id = GSM3198028 !Series_sample_id = GSM3198029 !Series_sample_id = GSM3198030 !Series_sample_id = GSM3198031 !Series_sample_id = GSM3198032 !Series_sample_id = GSM3198033 !Series_sample_id = GSM3198034 !Series_sample_id = GSM3198035 !Series_sample_id = GSM3198036 !Series_sample_id = GSM3198037 !Series_sample_id = GSM3198038 !Series_sample_id = GSM3198039 !Series_sample_id = GSM3198040 !Series_sample_id = GSM3198041 !Series_sample_id = GSM3198042 !Series_sample_id = GSM3198043 !Series_sample_id = GSM3198044 !Series_sample_id = GSM3198045 !Series_sample_id = GSM3198046 !Series_sample_id = GSM3198047 !Series_sample_id = GSM3198048 !Series_sample_id = GSM3198049 !Series_sample_id = GSM3198050 !Series_sample_id = GSM3198051 !Series_sample_id = GSM3198052 !Series_sample_id = GSM3198053 !Series_sample_id = GSM3198054 !Series_sample_id = GSM3198055 !Series_sample_id = GSM3198056 !Series_sample_id = GSM3198057 !Series_sample_id = GSM3198058 !Series_sample_id = GSM3198059 !Series_sample_id = GSM3198060 !Series_sample_id = GSM3198061 !Series_sample_id = GSM3198062 !Series_sample_id = GSM3198063 !Series_sample_id = GSM3198064 !Series_sample_id = GSM3198065 !Series_sample_id = GSM3198066 !Series_sample_id = GSM3198067 !Series_sample_id = GSM3198068 !Series_sample_id = GSM3198069 !Series_sample_id = GSM3198070 !Series_sample_id = GSM3198071 !Series_sample_id = GSM3198072 !Series_sample_id = GSM3198073 !Series_sample_id = GSM3198074 !Series_sample_id = GSM3198075 !Series_sample_id = GSM3198076 !Series_sample_id = GSM3198077 !Series_sample_id = GSM3198078 !Series_sample_id = GSM3198079 !Series_sample_id = GSM3198080 !Series_sample_id = GSM3198081 !Series_sample_id = GSM3198082 !Series_sample_id = GSM3198083 !Series_sample_id = GSM3198084 !Series_sample_id = GSM3198085 !Series_sample_id = GSM3198086 !Series_sample_id = GSM3198087 !Series_sample_id = GSM3198088 !Series_sample_id = GSM3198089 !Series_sample_id = GSM3198090 !Series_sample_id = GSM3198091 !Series_sample_id = GSM3198092 !Series_sample_id = GSM3198093 !Series_sample_id = GSM3198094 !Series_sample_id = GSM3198095 !Series_sample_id = GSM3198096 !Series_sample_id = GSM3198097 !Series_sample_id = GSM3198098 !Series_sample_id = GSM3198099 !Series_sample_id = GSM3198100 !Series_sample_id = GSM3198101 !Series_sample_id = GSM3198102 !Series_sample_id = GSM3198103 !Series_sample_id = GSM3198104 !Series_sample_id = GSM3198105 !Series_sample_id = GSM3198106 !Series_sample_id = GSM3198107 !Series_sample_id = GSM3198108 !Series_sample_id = GSM3198109 !Series_sample_id = GSM3198110 !Series_sample_id = GSM3198111 !Series_sample_id = GSM3198112 !Series_sample_id = GSM3198113 !Series_sample_id = GSM3198114 !Series_sample_id = GSM3198115 !Series_sample_id = GSM3198116 !Series_sample_id = GSM3198117 !Series_sample_id = GSM3198118 !Series_sample_id = GSM3198119 !Series_sample_id = GSM3198120 !Series_sample_id = GSM3198121 !Series_sample_id = GSM3198122 !Series_sample_id = GSM3198123 !Series_sample_id = GSM3198124 !Series_sample_id = GSM3198125 !Series_sample_id = GSM3198126 !Series_sample_id = GSM3198127 !Series_sample_id = GSM3198128 !Series_sample_id = GSM3198129 !Series_sample_id = GSM3198130 !Series_sample_id = GSM3198131 !Series_sample_id = GSM3198132 !Series_sample_id = GSM3198133 !Series_sample_id = GSM3198134 !Series_sample_id = GSM3198135 !Series_sample_id = GSM3198136 !Series_sample_id = GSM3198137 !Series_sample_id = GSM3198138 !Series_sample_id = GSM3198139 !Series_sample_id = GSM3198140 !Series_sample_id = GSM3198141 !Series_sample_id = GSM3198142 !Series_sample_id = GSM3198143 !Series_sample_id = GSM3198144 !Series_sample_id = GSM3198145 !Series_sample_id = GSM3198146 !Series_sample_id = GSM3198147 !Series_sample_id = GSM3198148 !Series_sample_id = GSM3198149 !Series_sample_id = GSM3198150 !Series_sample_id = GSM3198151 !Series_sample_id = GSM3198152 !Series_sample_id = GSM3198153 !Series_sample_id = GSM3198154 !Series_sample_id = GSM3198155 !Series_sample_id = GSM3198156 !Series_sample_id = GSM3198157 !Series_sample_id = GSM3198158 !Series_sample_id = GSM3198159 !Series_sample_id = GSM3198160 !Series_sample_id = GSM3198161 !Series_sample_id = GSM3198162 !Series_sample_id = GSM3198163 !Series_sample_id = GSM3198164 !Series_sample_id = GSM3198165 !Series_sample_id = GSM3198166 !Series_sample_id = GSM3198167 !Series_sample_id = GSM3198168 !Series_sample_id = GSM3198169 !Series_sample_id = GSM3198170 !Series_sample_id = GSM3198171 !Series_sample_id = GSM3198172 !Series_sample_id = GSM3198173 !Series_sample_id = GSM3198174 !Series_sample_id = GSM3198175 !Series_sample_id = GSM3198176 !Series_sample_id = GSM3198177 !Series_sample_id = GSM3198178 !Series_sample_id = GSM3198179 !Series_sample_id = GSM3198180 !Series_sample_id = GSM3198181 !Series_sample_id = GSM3198182 !Series_sample_id = GSM3198183 !Series_sample_id = GSM3198184 !Series_sample_id = GSM3198185 !Series_sample_id = GSM3198186 !Series_sample_id = GSM3198187 !Series_sample_id = GSM3198188 !Series_sample_id = GSM3198189 !Series_sample_id = GSM3198190 !Series_sample_id = GSM3198191 !Series_sample_id = GSM3198192 !Series_sample_id = GSM3198193 !Series_sample_id = GSM3198194 !Series_sample_id = GSM3198195 !Series_sample_id = GSM3198196 !Series_sample_id = GSM3198197 !Series_sample_id = GSM3198198 !Series_sample_id = GSM3198199 !Series_sample_id = GSM3198200 !Series_sample_id = GSM3198201 !Series_sample_id = GSM3198202 !Series_sample_id = GSM3198203 !Series_sample_id = GSM3198204 !Series_sample_id = GSM3198205 !Series_sample_id = GSM3198206 !Series_sample_id = GSM3198207 !Series_sample_id = GSM3198208 !Series_sample_id = GSM3198209 !Series_sample_id = GSM3198210 !Series_sample_id = GSM3198211 !Series_sample_id = GSM3198212 !Series_sample_id = GSM3198213 !Series_sample_id = GSM3198214 !Series_sample_id = GSM3198215 !Series_sample_id = GSM3198216 !Series_sample_id = GSM3198217 !Series_sample_id = GSM3198218 !Series_sample_id = GSM3198219 !Series_sample_id = GSM3198220 !Series_sample_id = GSM3198221 !Series_sample_id = GSM3198222 !Series_sample_id = GSM3198223 !Series_sample_id = GSM3198224 !Series_sample_id = GSM3198225 !Series_sample_id = GSM3198226 !Series_sample_id = GSM3198227 !Series_sample_id = GSM3198228 !Series_sample_id = GSM3198229 !Series_sample_id = GSM3198230 !Series_sample_id = GSM3198231 !Series_sample_id = GSM3198232 !Series_sample_id = GSM3198233 !Series_sample_id = GSM3198234 !Series_sample_id = GSM3198235 !Series_sample_id = GSM3198236 !Series_sample_id = GSM3198237 !Series_sample_id = GSM3198238 !Series_sample_id = GSM3198239 !Series_sample_id = GSM3198240 !Series_sample_id = GSM3198241 !Series_sample_id = GSM3198242 !Series_sample_id = GSM3198243 !Series_sample_id = GSM3198244 !Series_sample_id = GSM3198245 !Series_sample_id = GSM3198246 !Series_sample_id = GSM3198247 !Series_sample_id = GSM3198248 !Series_sample_id = GSM3198249 !Series_sample_id = GSM3198250 !Series_sample_id = GSM3198251 !Series_sample_id = GSM3198252 !Series_sample_id = GSM3198253 !Series_sample_id = GSM3198254 !Series_sample_id = GSM3198255 !Series_sample_id = GSM3198256 !Series_sample_id = GSM3198257 !Series_sample_id = GSM3198258 !Series_sample_id = GSM3198259 !Series_sample_id = GSM3198260 !Series_sample_id = GSM3198261 !Series_sample_id = GSM3198262 !Series_sample_id = GSM3198263 !Series_sample_id = GSM3198264 !Series_sample_id = GSM3198265 !Series_sample_id = GSM3198266 !Series_sample_id = GSM3198267 !Series_sample_id = GSM3198268 !Series_sample_id = GSM3198269 !Series_sample_id = GSM3198270 !Series_sample_id = GSM3198271 !Series_sample_id = GSM3198272 !Series_sample_id = GSM3198273 !Series_sample_id = GSM3198274 !Series_sample_id = GSM3198275 !Series_sample_id = GSM3198276 !Series_sample_id = GSM3198277 !Series_sample_id = GSM3198278 !Series_sample_id = GSM3198279 !Series_sample_id = GSM3198280 !Series_sample_id = GSM3198281 !Series_sample_id = GSM3198282 !Series_sample_id = GSM3198283 !Series_sample_id = GSM3198284 !Series_sample_id = GSM3198285 !Series_sample_id = GSM3198286 !Series_sample_id = GSM3198287 !Series_sample_id = GSM3198288 !Series_sample_id = GSM3198289 !Series_sample_id = GSM3198290 !Series_sample_id = GSM3198291 !Series_sample_id = GSM3198292 !Series_sample_id = GSM3198293 !Series_sample_id = GSM3198294 !Series_sample_id = GSM3198295 !Series_sample_id = GSM3198296 !Series_sample_id = GSM3198297 !Series_sample_id = GSM3198298 !Series_sample_id = GSM3198299 !Series_sample_id = GSM3198300 !Series_sample_id = GSM3198301 !Series_sample_id = GSM3198302 !Series_sample_id = GSM3198303 !Series_sample_id = GSM3198304 !Series_sample_id = GSM3198305 !Series_sample_id = GSM3198306 !Series_sample_id = GSM3198307 !Series_sample_id = GSM3198308 !Series_sample_id = GSM3198309 !Series_sample_id = GSM3198310 !Series_sample_id = GSM3198311 !Series_sample_id = GSM3198312 !Series_sample_id = GSM3198313 !Series_sample_id = GSM3198314 !Series_sample_id = GSM3198315 !Series_sample_id = GSM3198316 !Series_sample_id = GSM3198317 !Series_sample_id = GSM3198318 !Series_sample_id = GSM3198319 !Series_sample_id = GSM3198320 !Series_sample_id = GSM3198321 !Series_sample_id = GSM3198322 !Series_sample_id = GSM3198323 !Series_sample_id = GSM3198324 !Series_sample_id = GSM3198325 !Series_sample_id = GSM3198326 !Series_sample_id = GSM3198327 !Series_sample_id = GSM3198328 !Series_sample_id = GSM3198329 !Series_sample_id = GSM3198330 !Series_sample_id = GSM3198331 !Series_sample_id = GSM3198332 !Series_sample_id = GSM3198333 !Series_sample_id = GSM3198334 !Series_sample_id = GSM3198335 !Series_sample_id = GSM3198336 !Series_sample_id = GSM3198337 !Series_sample_id = GSM3198338 !Series_sample_id = GSM3198339 !Series_sample_id = GSM3198340 !Series_sample_id = GSM3198341 !Series_sample_id = GSM3198342 !Series_sample_id = GSM3198343 !Series_sample_id = GSM3198344 !Series_sample_id = GSM3198345 !Series_sample_id = GSM3198346 !Series_sample_id = GSM3198347 !Series_sample_id = GSM3198348 !Series_sample_id = GSM3198349 !Series_sample_id = GSM3198350 !Series_sample_id = GSM3198351 !Series_sample_id = GSM3198352 !Series_sample_id = GSM3198353 !Series_sample_id = GSM3198354 !Series_sample_id = GSM3198355 !Series_sample_id = GSM3198356 !Series_sample_id = GSM3198357 !Series_sample_id = GSM3198358 !Series_sample_id = GSM3198359 !Series_sample_id = GSM3198360 !Series_sample_id = GSM3198361 !Series_sample_id = GSM3198362 !Series_sample_id = GSM3198363 !Series_sample_id = GSM3198364 !Series_sample_id = GSM3198365 !Series_sample_id = GSM3198366 !Series_sample_id = GSM3198367 !Series_sample_id = GSM3198368 !Series_sample_id = GSM3198369 !Series_sample_id = GSM3198370 !Series_sample_id = GSM3198371 !Series_sample_id = GSM3198372 !Series_sample_id = GSM3198373 !Series_sample_id = GSM3198374 !Series_sample_id = GSM3198375 !Series_sample_id = GSM3198376 !Series_sample_id = GSM3198377 !Series_sample_id = GSM3198378 !Series_sample_id = GSM3198379 !Series_sample_id = GSM3198380 !Series_sample_id = GSM3198381 !Series_sample_id = GSM3198382 !Series_sample_id = GSM3198383 !Series_sample_id = GSM3198384 !Series_sample_id = GSM3198385 !Series_sample_id = GSM3198386 !Series_sample_id = GSM3198387 !Series_sample_id = GSM3198388 !Series_sample_id = GSM3198389 !Series_sample_id = GSM3198390 !Series_sample_id = GSM3198391 !Series_sample_id = GSM3198392 !Series_sample_id = GSM3198393 !Series_sample_id = GSM3198394 !Series_sample_id = GSM3198395 !Series_sample_id = GSM3198396 !Series_sample_id = GSM3198397 !Series_sample_id = GSM3198398 !Series_sample_id = GSM3198399 !Series_sample_id = GSM3198400 !Series_sample_id = GSM3198401 !Series_sample_id = GSM3198402 !Series_sample_id = GSM3198403 !Series_sample_id = GSM3198404 !Series_sample_id = GSM3198405 !Series_sample_id = GSM3198406 !Series_sample_id = GSM3198407 !Series_sample_id = GSM3198408 !Series_sample_id = GSM3198409 !Series_sample_id = GSM3198410 !Series_sample_id = GSM3198411 !Series_sample_id = GSM3198412 !Series_sample_id = GSM3198413 !Series_sample_id = GSM3198414 !Series_sample_id = GSM3198415 !Series_sample_id = GSM3198416 !Series_sample_id = GSM3198417 !Series_sample_id = GSM3198418 !Series_sample_id = GSM3198419 !Series_sample_id = GSM3198420 !Series_sample_id = GSM3198421 !Series_sample_id = GSM3198422 !Series_sample_id = GSM3198423 !Series_sample_id = GSM3198424 !Series_sample_id = GSM3198425 !Series_sample_id = GSM3198426 !Series_sample_id = GSM3198427 !Series_sample_id = GSM3198428 !Series_sample_id = GSM3198429 !Series_sample_id = GSM3198430 !Series_sample_id = GSM3198431 !Series_sample_id = GSM3198432 !Series_sample_id = GSM3198433 !Series_sample_id = GSM3198434 !Series_sample_id = GSM3198435 !Series_sample_id = GSM3198436 !Series_sample_id = GSM3198437 !Series_sample_id = GSM3198438 !Series_sample_id = GSM3198439 !Series_sample_id = GSM3198440 !Series_sample_id = GSM3198441 !Series_sample_id = GSM3198442 !Series_sample_id = GSM3198443 !Series_sample_id = GSM3198444 !Series_sample_id = GSM3198445 !Series_sample_id = GSM3198446 !Series_sample_id = GSM3198447 !Series_sample_id = GSM3198448 !Series_sample_id = GSM3198449 !Series_sample_id = GSM3198450 !Series_sample_id = GSM3198451 !Series_sample_id = GSM3198452 !Series_sample_id = GSM3198453 !Series_sample_id = GSM3198454 !Series_sample_id = GSM3198455 !Series_sample_id = GSM3198456 !Series_sample_id = GSM3198457 !Series_sample_id = GSM3198458 !Series_sample_id = GSM3198459 !Series_sample_id = GSM3198460 !Series_sample_id = GSM3198461 !Series_sample_id = GSM3198462 !Series_sample_id = GSM3198463 !Series_sample_id = GSM3198464 !Series_sample_id = GSM3198465 !Series_sample_id = GSM3198466 !Series_sample_id = GSM3198467 !Series_sample_id = GSM3198468 !Series_sample_id = GSM3198469 !Series_sample_id = GSM3198470 !Series_sample_id = GSM3198471 !Series_sample_id = GSM3198472 !Series_sample_id = GSM3198473 !Series_sample_id = GSM3198474 !Series_sample_id = GSM3198475 !Series_sample_id = GSM3198476 !Series_sample_id = GSM3198477 !Series_sample_id = GSM3198478 !Series_sample_id = GSM3198479 !Series_sample_id = GSM3198480 !Series_sample_id = GSM3198481 !Series_sample_id = GSM3198482 !Series_sample_id = GSM3198483 !Series_sample_id = GSM3198484 !Series_sample_id = GSM3198485 !Series_sample_id = GSM3198486 !Series_sample_id = GSM3198487 !Series_sample_id = GSM3198488 !Series_sample_id = GSM3198489 !Series_sample_id = GSM3198490 !Series_sample_id = GSM3198491 !Series_sample_id = GSM3198492 !Series_sample_id = GSM3198493 !Series_sample_id = GSM3198494 !Series_sample_id = GSM3198495 !Series_sample_id = GSM3198496 !Series_sample_id = GSM3198497 !Series_sample_id = GSM3198498 !Series_sample_id = GSM3198499 !Series_sample_id = GSM3198500 !Series_sample_id = GSM3198501 !Series_sample_id = GSM3198502 !Series_sample_id = GSM3198503 !Series_sample_id = GSM3198504 !Series_sample_id = GSM3198505 !Series_sample_id = GSM3198506 !Series_sample_id = GSM3198507 !Series_sample_id = GSM3198508 !Series_sample_id = GSM3198509 !Series_sample_id = GSM3198510 !Series_sample_id = GSM3198511 !Series_sample_id = GSM3198512 !Series_sample_id = GSM3198513 !Series_sample_id = GSM3198514 !Series_sample_id = GSM3198515 !Series_sample_id = GSM3198516 !Series_sample_id = GSM3198517 !Series_sample_id = GSM3198518 !Series_sample_id = GSM3198519 !Series_sample_id = GSM3198520 !Series_sample_id = GSM3198521 !Series_sample_id = GSM3198522 !Series_sample_id = GSM3198523 !Series_sample_id = GSM3198524 !Series_sample_id = GSM3198525 !Series_sample_id = GSM3198526 !Series_sample_id = GSM3198527 !Series_sample_id = GSM3198528 !Series_sample_id = GSM3198529 !Series_sample_id = GSM3198530 !Series_sample_id = GSM3198531 !Series_sample_id = GSM3198532 !Series_sample_id = GSM3198533 !Series_sample_id = GSM3198534 !Series_sample_id = GSM3198535 !Series_sample_id = GSM3198536 !Series_sample_id = GSM3198537 !Series_sample_id = GSM3198538 !Series_sample_id = GSM3198539 !Series_sample_id = GSM3198540 !Series_sample_id = GSM3198541 !Series_sample_id = GSM3198542 !Series_sample_id = GSM3198543 !Series_sample_id = GSM3198544 !Series_sample_id = GSM3198545 !Series_sample_id = GSM3198546 !Series_sample_id = GSM3198547 !Series_sample_id = GSM3198548 !Series_sample_id = GSM3198549 !Series_sample_id = GSM3198550 !Series_sample_id = GSM3198551 !Series_sample_id = GSM3198552 !Series_sample_id = GSM3198553 !Series_sample_id = GSM3198554 !Series_sample_id = GSM3198555 !Series_sample_id = GSM3198556 !Series_sample_id = GSM3198557 !Series_sample_id = GSM3198558 !Series_sample_id = GSM3198559 !Series_sample_id = GSM3198560 !Series_sample_id = GSM3198561 !Series_sample_id = GSM3198562 !Series_sample_id = GSM3198563 !Series_sample_id = GSM3198564 !Series_sample_id = GSM3198565 !Series_sample_id = GSM3198566 !Series_sample_id = GSM3198567 !Series_sample_id = GSM3198568 !Series_sample_id = GSM3198569 !Series_sample_id = GSM3198570 !Series_sample_id = GSM3198571 !Series_sample_id = GSM3198572 !Series_sample_id = GSM3198573 !Series_sample_id = GSM3198574 !Series_sample_id = GSM3198575 !Series_sample_id = GSM3198576 !Series_sample_id = GSM3198577 !Series_sample_id = GSM3198578 !Series_sample_id = GSM3198579 !Series_sample_id = GSM3198580 !Series_sample_id = GSM3198581 !Series_sample_id = GSM3198582 !Series_sample_id = GSM3198583 !Series_sample_id = GSM3198584 !Series_sample_id = GSM3198585 !Series_sample_id = GSM3198586 !Series_sample_id = GSM3198587 !Series_sample_id = GSM3198588 !Series_sample_id = GSM3198589 !Series_sample_id = GSM3198590 !Series_sample_id = GSM3198591 !Series_sample_id = GSM3198592 !Series_sample_id = GSM3198593 !Series_sample_id = GSM3198594 !Series_sample_id = GSM3198595 !Series_sample_id = GSM3198596 !Series_sample_id = GSM3198597 !Series_sample_id = GSM3198598 !Series_sample_id = GSM3198599 !Series_sample_id = GSM3198600 !Series_sample_id = GSM3198601 !Series_sample_id = GSM3198602 !Series_sample_id = GSM3198603 !Series_sample_id = GSM3198604 !Series_sample_id = GSM3198605 !Series_sample_id = GSM3198606 !Series_sample_id = GSM3198607 !Series_sample_id = GSM3198608 !Series_sample_id = GSM3198609 !Series_sample_id = GSM3198610 !Series_sample_id = GSM3198611 !Series_sample_id = GSM3198612 !Series_sample_id = GSM3198613 !Series_sample_id = GSM3198614 !Series_sample_id = GSM3198615 !Series_sample_id = GSM3198616 !Series_sample_id = GSM3198617 !Series_sample_id = GSM3198618 !Series_sample_id = GSM3198619 !Series_sample_id = GSM3198620 !Series_sample_id = GSM3198621 !Series_sample_id = GSM3198622 !Series_sample_id = GSM3198623 !Series_sample_id = GSM3198624 !Series_sample_id = GSM3198625 !Series_sample_id = GSM3198626 !Series_sample_id = GSM3198627 !Series_sample_id = GSM3198628 !Series_sample_id = GSM3198629 !Series_sample_id = GSM3198630 !Series_sample_id = GSM3198631 !Series_sample_id = GSM3198632 !Series_sample_id = GSM3198633 !Series_sample_id = GSM3198634 !Series_sample_id = GSM3198635 !Series_sample_id = GSM3198636 !Series_sample_id = GSM3198637 !Series_sample_id = GSM3198638 !Series_sample_id = GSM3198639 !Series_sample_id = GSM3198640 !Series_sample_id = GSM3198641 !Series_sample_id = GSM3198642 !Series_sample_id = GSM3198643 !Series_sample_id = GSM3198644 !Series_sample_id = GSM3198645 !Series_sample_id = GSM3198646 !Series_sample_id = GSM3198647 !Series_sample_id = GSM3198648 !Series_sample_id = GSM3198649 !Series_sample_id = GSM3198650 !Series_sample_id = GSM3198651 !Series_sample_id = GSM3198652 !Series_sample_id = GSM3198653 !Series_sample_id = GSM3198654 !Series_sample_id = GSM3198655 !Series_sample_id = GSM3198656 !Series_sample_id = GSM3198657 !Series_sample_id = GSM3198658 !Series_sample_id = GSM3198659 !Series_sample_id = GSM3198660 !Series_sample_id = GSM3198661 !Series_sample_id = GSM3198662 !Series_sample_id = GSM3198663 !Series_sample_id = GSM3198664 !Series_sample_id = GSM3198665 !Series_sample_id = GSM3198666 !Series_sample_id = GSM3198667 !Series_sample_id = GSM3198668 !Series_sample_id = GSM3198669 !Series_sample_id = GSM3198670 !Series_sample_id = GSM3198671 !Series_sample_id = GSM3198672 !Series_sample_id = GSM3198673 !Series_sample_id = GSM3198674 !Series_sample_id = GSM3198675 !Series_sample_id = GSM3198676 !Series_sample_id = GSM3198677 !Series_sample_id = GSM3198678 !Series_sample_id = GSM3198679 !Series_sample_id = GSM3198680 !Series_sample_id = GSM3198681 !Series_sample_id = GSM3198682 !Series_sample_id = GSM3198683 !Series_sample_id = GSM3198684 !Series_sample_id = GSM3198685 !Series_sample_id = GSM3198686 !Series_sample_id = GSM3198687 !Series_sample_id = GSM3198688 !Series_sample_id = GSM3198689 !Series_sample_id = GSM3198690 !Series_sample_id = GSM3198691 !Series_sample_id = GSM3198692 !Series_sample_id = GSM3198693 !Series_sample_id = GSM3198694 !Series_sample_id = GSM3198695 !Series_sample_id = GSM3198696 !Series_sample_id = GSM3198697 !Series_sample_id = GSM3198698 !Series_sample_id = GSM3198699 !Series_sample_id = GSM3198700 !Series_sample_id = GSM3198701 !Series_sample_id = GSM3198702 !Series_sample_id = GSM3198703 !Series_sample_id = GSM3198704 !Series_sample_id = GSM3198705 !Series_sample_id = GSM3198706 !Series_sample_id = GSM3198707 !Series_sample_id = GSM3198708 !Series_sample_id = GSM3198709 !Series_sample_id = GSM3198710 !Series_sample_id = GSM3198711 !Series_sample_id = GSM3198712 !Series_sample_id = GSM3198713 !Series_sample_id = GSM3198714 !Series_sample_id = GSM3198715 !Series_sample_id = GSM3198716 !Series_sample_id = GSM3198717 !Series_sample_id = GSM3198718 !Series_sample_id = GSM3198719 !Series_sample_id = GSM3198720 !Series_sample_id = GSM3198721 !Series_sample_id = GSM3198722 !Series_sample_id = GSM3198723 !Series_sample_id = GSM3198724 !Series_sample_id = GSM3198725 !Series_sample_id = GSM3198726 !Series_sample_id = GSM3198727 !Series_sample_id = GSM3198728 !Series_sample_id = GSM3198729 !Series_sample_id = GSM3198730 !Series_sample_id = GSM3198731 !Series_sample_id = GSM3198732 !Series_sample_id = GSM3198733 !Series_sample_id = GSM3198734 !Series_sample_id = GSM3198735 !Series_sample_id = GSM3198736 !Series_sample_id = GSM3198737 !Series_sample_id = GSM3198738 !Series_sample_id = GSM3198739 !Series_sample_id = GSM3198740 !Series_sample_id = GSM3198741 !Series_sample_id = GSM3198742 !Series_sample_id = GSM3198743 !Series_sample_id = GSM3198744 !Series_sample_id = GSM3198745 !Series_sample_id = GSM3198746 !Series_sample_id = GSM3198747 !Series_sample_id = GSM3198748 !Series_sample_id = GSM3198749 !Series_sample_id = GSM3198750 !Series_sample_id = GSM3198751 !Series_sample_id = GSM3198752 !Series_sample_id = GSM3198753 !Series_sample_id = GSM3198754 !Series_sample_id = GSM3198755 !Series_sample_id = GSM3198756 !Series_sample_id = GSM3198757 !Series_sample_id = GSM3198758 !Series_sample_id = GSM3198759 !Series_sample_id = GSM3198760 !Series_sample_id = GSM3198761 !Series_sample_id = GSM3198762 !Series_sample_id = GSM3198763 !Series_sample_id = GSM3198764 !Series_sample_id = GSM3198765 !Series_sample_id = GSM3198766 !Series_sample_id = GSM3198767 !Series_sample_id = GSM3198768 !Series_sample_id = GSM3198769 !Series_sample_id = GSM3198770 !Series_sample_id = GSM3198771 !Series_sample_id = GSM3198772 !Series_sample_id = GSM3198773 !Series_sample_id = GSM3198774 !Series_sample_id = GSM3198775 !Series_sample_id = GSM3198776 !Series_sample_id = GSM3198777 !Series_sample_id = GSM3198778 !Series_sample_id = GSM3198779 !Series_sample_id = GSM3198780 !Series_sample_id = GSM3198781 !Series_sample_id = GSM3198782 !Series_sample_id = GSM3198783 !Series_sample_id = GSM3198784 !Series_sample_id = GSM3198785 !Series_sample_id = GSM3198786 !Series_sample_id = GSM3198787 !Series_sample_id = GSM3198788 !Series_sample_id = GSM3198789 !Series_sample_id = GSM3198790 !Series_sample_id = GSM3198791 !Series_sample_id = GSM3198792 !Series_sample_id = GSM3198793 !Series_sample_id = GSM3198794 !Series_sample_id = GSM3198795 !Series_sample_id = GSM3198796 !Series_sample_id = GSM3198797 !Series_sample_id = GSM3198798 !Series_sample_id = GSM3198799 !Series_sample_id = GSM3198800 !Series_sample_id = GSM3198801 !Series_sample_id = GSM3198802 !Series_sample_id = GSM3198803 !Series_sample_id = GSM3198804 !Series_sample_id = GSM3198805 !Series_sample_id = GSM3198806 !Series_sample_id = GSM3198807 !Series_sample_id = GSM3198808 !Series_sample_id = GSM3198809 !Series_sample_id = GSM3198810 !Series_sample_id = GSM3198811 !Series_sample_id = GSM3198812 !Series_sample_id = GSM3198813 !Series_sample_id = GSM3198814 !Series_sample_id = GSM3198815 !Series_sample_id = GSM3198816 !Series_sample_id = GSM3198817 !Series_sample_id = GSM3198818 !Series_sample_id = GSM3198819 !Series_sample_id = GSM3198820 !Series_sample_id = GSM3198821 !Series_sample_id = GSM3198822 !Series_sample_id = GSM3198823 !Series_sample_id = GSM3198824 !Series_sample_id = GSM3198825 !Series_sample_id = GSM3198826 !Series_sample_id = GSM3198827 !Series_sample_id = GSM3198828 !Series_sample_id = GSM3198829 !Series_sample_id = GSM3198830 !Series_sample_id = GSM3198831 !Series_sample_id = GSM3198832 !Series_sample_id = GSM3198833 !Series_sample_id = GSM3198834 !Series_sample_id = GSM3198835 !Series_sample_id = GSM3198836 !Series_sample_id = GSM3198837 !Series_sample_id = GSM3198838 !Series_sample_id = GSM3198839 !Series_sample_id = GSM3198840 !Series_sample_id = GSM3198841 !Series_sample_id = GSM3198842 !Series_sample_id = GSM3198843 !Series_sample_id = GSM3198844 !Series_sample_id = GSM3198845 !Series_sample_id = GSM3198846 !Series_sample_id = GSM3198847 !Series_sample_id = GSM3198848 !Series_sample_id = GSM3198849 !Series_sample_id = GSM3198850 !Series_sample_id = GSM3198851 !Series_sample_id = GSM3198852 !Series_sample_id = GSM3198853 !Series_sample_id = GSM3198854 !Series_sample_id = GSM3198855 !Series_sample_id = GSM3198856 !Series_sample_id = GSM3198857 !Series_sample_id = GSM3198858 !Series_sample_id = GSM3198859 !Series_sample_id = GSM3198860 !Series_sample_id = GSM3198861 !Series_sample_id = GSM3198862 !Series_sample_id = GSM3198863 !Series_sample_id = GSM3198864 !Series_sample_id = GSM3198865 !Series_sample_id = GSM3198866 !Series_sample_id = GSM3198867 !Series_sample_id = GSM3198868 !Series_sample_id = GSM3198869 !Series_sample_id = GSM3198870 !Series_sample_id = GSM3198871 !Series_sample_id = GSM3198872 !Series_sample_id = GSM3198873 !Series_sample_id = GSM3198874 !Series_sample_id = GSM3198875 !Series_sample_id = GSM3198876 !Series_sample_id = GSM3198877 !Series_sample_id = GSM3198878 !Series_sample_id = GSM3198879 !Series_sample_id = GSM3198880 !Series_sample_id = GSM3198881 !Series_sample_id = GSM3198882 !Series_sample_id = GSM3198883 !Series_sample_id = GSM3198884 !Series_sample_id = GSM3198885 !Series_sample_id = GSM3198886 !Series_sample_id = GSM3198887 !Series_sample_id = GSM3198888 !Series_sample_id = GSM3198889 !Series_sample_id = GSM3198890 !Series_sample_id = GSM3198891 !Series_sample_id = GSM3198892 !Series_sample_id = GSM3198893 !Series_sample_id = GSM3198894 !Series_sample_id = GSM3198895 !Series_sample_id = GSM3198896 !Series_sample_id = GSM3198897 !Series_sample_id = GSM3198898 !Series_sample_id = GSM3198899 !Series_sample_id = GSM3198900 !Series_sample_id = GSM3198901 !Series_sample_id = GSM3198902 !Series_sample_id = GSM3198903 !Series_sample_id = GSM3198904 !Series_sample_id = GSM3198905 !Series_sample_id = GSM3198906 !Series_sample_id = GSM3198907 !Series_sample_id = GSM3198908 !Series_sample_id = GSM3198909 !Series_sample_id = GSM3198910 !Series_sample_id = GSM3198911 !Series_sample_id = GSM3198912 !Series_sample_id = GSM3198913 !Series_sample_id = GSM3198914 !Series_sample_id = GSM3198915 !Series_sample_id = GSM3198916 !Series_sample_id = GSM3198917 !Series_sample_id = GSM3198918 !Series_sample_id = GSM3198919 !Series_sample_id = GSM3198920 !Series_sample_id = GSM3198921 !Series_sample_id = GSM3198922 !Series_sample_id = GSM3198923 !Series_sample_id = GSM3198924 !Series_sample_id = GSM3198925 !Series_sample_id = GSM3198926 !Series_sample_id = GSM3198927 !Series_sample_id = GSM3198928 !Series_sample_id = GSM3198929 !Series_sample_id = GSM3198930 !Series_sample_id = GSM3198931 !Series_sample_id = GSM3198932 !Series_sample_id = GSM3198933 !Series_sample_id = GSM3198934 !Series_sample_id = GSM3198935 !Series_sample_id = GSM3198936 !Series_sample_id = GSM3198937 !Series_sample_id = GSM3198938 !Series_sample_id = GSM3198939 !Series_sample_id = GSM3198940 !Series_sample_id = GSM3198941 !Series_sample_id = GSM3198942 !Series_sample_id = GSM3198943 !Series_sample_id = GSM3198944 !Series_sample_id = GSM3198945 !Series_sample_id = GSM3198946 !Series_sample_id = GSM3198947 !Series_sample_id = GSM3198948 !Series_sample_id = GSM3198949 !Series_sample_id = GSM3198950 !Series_sample_id = GSM3198951 !Series_sample_id = GSM3198952 !Series_sample_id = GSM3198953 !Series_sample_id = GSM3198954 !Series_sample_id = GSM3198955 !Series_sample_id = GSM3198956 !Series_sample_id = GSM3198957 !Series_sample_id = GSM3198958 !Series_sample_id = GSM3198959 !Series_sample_id = GSM3198960 !Series_sample_id = GSM3198961 !Series_sample_id = GSM3198962 !Series_sample_id = GSM3198963 !Series_sample_id = GSM3198964 !Series_sample_id = GSM3198965 !Series_sample_id = GSM3198966 !Series_sample_id = GSM3198967 !Series_sample_id = GSM3198968 !Series_sample_id = GSM3198969 !Series_sample_id = GSM3198970 !Series_sample_id = GSM3198971 !Series_sample_id = GSM3198972 !Series_sample_id = GSM3198973 !Series_sample_id = GSM3198974 !Series_sample_id = GSM3198975 !Series_sample_id = GSM3198976 !Series_sample_id = GSM3198977 !Series_sample_id = GSM3198978 !Series_sample_id = GSM3198979 !Series_sample_id = GSM3198980 !Series_sample_id = GSM3198981 !Series_sample_id = GSM3198982 !Series_sample_id = GSM3198983 !Series_sample_id = GSM3198984 !Series_sample_id = GSM3198985 !Series_sample_id = GSM3198986 !Series_sample_id = GSM3198987 !Series_sample_id = GSM3198988 !Series_sample_id = GSM3198989 !Series_sample_id = GSM3198990 !Series_sample_id = GSM3198991 !Series_sample_id = GSM3198992 !Series_sample_id = GSM3198993 !Series_sample_id = GSM3198994 !Series_sample_id = GSM3198995 !Series_sample_id = GSM3198996 !Series_sample_id = GSM3198997 !Series_sample_id = GSM3198998 !Series_sample_id = GSM3198999 !Series_sample_id = GSM3199000 !Series_sample_id = GSM3199001 !Series_sample_id = GSM3199002 !Series_sample_id = GSM3199003 !Series_sample_id = GSM3199004 !Series_sample_id = GSM3199005 !Series_sample_id = GSM3199006 !Series_sample_id = GSM3199007 !Series_sample_id = GSM3199008 !Series_sample_id = GSM3199009 !Series_sample_id = GSM3199010 !Series_sample_id = GSM3199011 !Series_sample_id = GSM3199012 !Series_sample_id = GSM3199013 !Series_sample_id = GSM3199014 !Series_sample_id = GSM3199015 !Series_sample_id = GSM3199016 !Series_sample_id = GSM3199017 !Series_sample_id = GSM3199018 !Series_sample_id = GSM3199019 !Series_sample_id = GSM3199020 !Series_sample_id = GSM3199021 !Series_sample_id = GSM3199022 !Series_sample_id = GSM3199023 !Series_sample_id = GSM3199024 !Series_sample_id = GSM3199025 !Series_sample_id = GSM3199026 !Series_sample_id = GSM3199027 !Series_sample_id = GSM3199028 !Series_sample_id = GSM3199029 !Series_sample_id = GSM3199030 !Series_sample_id = GSM3199031 !Series_sample_id = GSM3199032 !Series_sample_id = GSM3199033 !Series_sample_id = GSM3199034 !Series_sample_id = GSM3199035 !Series_sample_id = GSM3199036 !Series_sample_id = GSM3199037 !Series_sample_id = GSM3199038 !Series_sample_id = GSM3199039 !Series_sample_id = GSM3199040 !Series_sample_id = GSM3199041 !Series_sample_id = GSM3199042 !Series_sample_id = GSM3199043 !Series_sample_id = GSM3199044 !Series_sample_id = GSM3199045 !Series_sample_id = GSM3199046 !Series_sample_id = GSM3199047 !Series_sample_id = GSM3199048 !Series_sample_id = GSM3199049 !Series_sample_id = GSM3199050 !Series_sample_id = GSM3199051 !Series_sample_id = GSM3199052 !Series_sample_id = GSM3199053 !Series_sample_id = GSM3199054 !Series_sample_id = GSM3199055 !Series_sample_id = GSM3199056 !Series_sample_id = GSM3199057 !Series_sample_id = GSM3199058 !Series_sample_id = GSM3199059 !Series_sample_id = GSM3199060 !Series_sample_id = GSM3199061 !Series_sample_id = GSM3199062 !Series_sample_id = GSM3199063 !Series_sample_id = GSM3199064 !Series_sample_id = GSM3199065 !Series_sample_id = GSM3199066 !Series_sample_id = GSM3199067 !Series_sample_id = GSM3199068 !Series_sample_id = GSM3199069 !Series_sample_id = GSM3199070 !Series_sample_id = GSM3199071 !Series_sample_id = GSM3199072 !Series_sample_id = GSM3199073 !Series_sample_id = GSM3199074 !Series_sample_id = GSM3199075 !Series_sample_id = GSM3199076 !Series_sample_id = GSM3199077 !Series_sample_id = GSM3199078 !Series_sample_id = GSM3199079 !Series_sample_id = GSM3199080 !Series_sample_id = GSM3199081 !Series_sample_id = GSM3199082 !Series_sample_id = GSM3199083 !Series_sample_id = GSM3199084 !Series_sample_id = GSM3199085 !Series_sample_id = GSM3199086 !Series_sample_id = GSM3199087 !Series_sample_id = GSM3199088 !Series_sample_id = GSM3199089 !Series_sample_id = GSM3199090 !Series_sample_id = GSM3199091 !Series_sample_id = GSM3199092 !Series_sample_id = GSM3199093 !Series_sample_id = GSM3199094 !Series_sample_id = GSM3199095 !Series_sample_id = GSM3199096 !Series_sample_id = GSM3199097 !Series_sample_id = GSM3199098 !Series_sample_id = GSM3199099 !Series_sample_id = GSM3199100 !Series_sample_id = GSM3199101 !Series_sample_id = GSM3199102 !Series_sample_id = GSM3199103 !Series_sample_id = GSM3199104 !Series_sample_id = GSM3199105 !Series_sample_id = GSM3199106 !Series_sample_id = GSM3199107 !Series_sample_id = GSM3199108 !Series_sample_id = GSM3199109 !Series_sample_id = GSM3199110 !Series_sample_id = GSM3199111 !Series_sample_id = GSM3199112 !Series_sample_id = GSM3199113 !Series_sample_id = GSM3199114 !Series_sample_id = GSM3199115 !Series_sample_id = GSM3199116 !Series_sample_id = GSM3199117 !Series_sample_id = GSM3199118 !Series_sample_id = GSM3199119 !Series_sample_id = GSM3199120 !Series_sample_id = GSM3199121 !Series_sample_id = GSM3199122 !Series_sample_id = GSM3199123 !Series_sample_id = GSM3199124 !Series_sample_id = GSM3199125 !Series_sample_id = GSM3199126 !Series_sample_id = GSM3199127 !Series_sample_id = GSM3199128 !Series_sample_id = GSM3199129 !Series_sample_id = GSM3199130 !Series_sample_id = GSM3199131 !Series_sample_id = GSM3199132 !Series_sample_id = GSM3199133 !Series_sample_id = GSM3199134 !Series_sample_id = GSM3199135 !Series_sample_id = GSM3199136 !Series_sample_id = GSM3199137 !Series_sample_id = GSM3199138 !Series_sample_id = GSM3199139 !Series_sample_id = GSM3199140 !Series_sample_id = GSM3199141 !Series_sample_id = GSM3199142 !Series_sample_id = GSM3199143 !Series_sample_id = GSM3199144 !Series_sample_id = GSM3199145 !Series_sample_id = GSM3199146 !Series_sample_id = GSM3199147 !Series_sample_id = GSM3199148 !Series_sample_id = GSM3199149 !Series_sample_id = GSM3199150 !Series_sample_id = GSM3199151 !Series_sample_id = GSM3199152 !Series_sample_id = GSM3199153 !Series_sample_id = GSM3199154 !Series_sample_id = GSM3199155 !Series_sample_id = GSM3199156 !Series_sample_id = GSM3199157 !Series_sample_id = GSM3199158 !Series_sample_id = GSM3199159 !Series_sample_id = GSM3199160 !Series_sample_id = GSM3199161 !Series_sample_id = GSM3199162 !Series_sample_id = GSM3199163 !Series_sample_id = GSM3199164 !Series_sample_id = GSM3199165 !Series_sample_id = GSM3199166 !Series_sample_id = GSM3199167 !Series_sample_id = GSM3199168 !Series_sample_id = GSM3199169 !Series_sample_id = GSM3199170 !Series_sample_id = GSM3199171 !Series_sample_id = GSM3199172 !Series_sample_id = GSM3199173 !Series_sample_id = GSM3199174 !Series_sample_id = GSM3199175 !Series_sample_id = GSM3199176 !Series_sample_id = GSM3199177 !Series_sample_id = GSM3199178 !Series_sample_id = GSM3199179 !Series_sample_id = GSM3199180 !Series_sample_id = GSM3199181 !Series_sample_id = GSM3199182 !Series_sample_id = GSM3199183 !Series_sample_id = GSM3199184 !Series_sample_id = GSM3199185 !Series_sample_id = GSM3199186 !Series_sample_id = GSM3199187 !Series_sample_id = GSM3199188 !Series_sample_id = GSM3199189 !Series_sample_id = GSM3199190 !Series_sample_id = GSM3199191 !Series_sample_id = GSM3199192 !Series_sample_id = GSM3199193 !Series_sample_id = GSM3199194 !Series_sample_id = GSM3199195 !Series_sample_id = GSM3199196 !Series_sample_id = GSM3199197 !Series_sample_id = GSM3199198 !Series_sample_id = GSM3199199 !Series_sample_id = GSM3199200 !Series_sample_id = GSM3199201 !Series_sample_id = GSM3199202 !Series_sample_id = GSM3199203 !Series_sample_id = GSM3199204 !Series_sample_id = GSM3199205 !Series_sample_id = GSM3199206 !Series_sample_id = GSM3199207 !Series_sample_id = GSM3199208 !Series_sample_id = GSM3199209 !Series_sample_id = GSM3199210 !Series_sample_id = GSM3199211 !Series_sample_id = GSM3199212 !Series_sample_id = GSM3199213 !Series_sample_id = GSM3199214 !Series_sample_id = GSM3199215 !Series_sample_id = GSM3199216 !Series_sample_id = GSM3199217 !Series_sample_id = GSM3199218 !Series_sample_id = GSM3199219 !Series_sample_id = GSM3199220 !Series_sample_id = GSM3199221 !Series_sample_id = GSM3199222 !Series_sample_id = GSM3199223 !Series_sample_id = GSM3199224 !Series_sample_id = GSM3199225 !Series_sample_id = GSM3199226 !Series_sample_id = GSM3199227 !Series_sample_id = GSM3199228 !Series_sample_id = GSM3199229 !Series_sample_id = GSM3199230 !Series_sample_id = GSM3199231 !Series_sample_id = GSM3199232 !Series_sample_id = GSM3199233 !Series_sample_id = GSM3199234 !Series_sample_id = GSM3199235 !Series_sample_id = GSM3199236 !Series_sample_id = GSM3199237 !Series_sample_id = GSM3199238 !Series_sample_id = GSM3199239 !Series_sample_id = GSM3199240 !Series_sample_id = GSM3199241 !Series_sample_id = GSM3199242 !Series_sample_id = GSM3199243 !Series_sample_id = GSM3199244 !Series_sample_id = GSM3199245 !Series_sample_id = GSM3199246 !Series_sample_id = GSM3199247 !Series_sample_id = GSM3199248 !Series_sample_id = GSM3199249 !Series_sample_id = GSM3199250 !Series_sample_id = GSM3199251 !Series_sample_id = GSM3199252 !Series_sample_id = GSM3199253 !Series_sample_id = GSM3199254 !Series_sample_id = GSM3199255 !Series_sample_id = GSM3199256 !Series_sample_id = GSM3199257 !Series_sample_id = GSM3199258 !Series_sample_id = GSM3199259 !Series_sample_id = GSM3199260 !Series_sample_id = GSM3199261 !Series_sample_id = GSM3199262 !Series_sample_id = GSM3199263 !Series_sample_id = GSM3199264 !Series_sample_id = GSM3199265 !Series_sample_id = GSM3199266 !Series_sample_id = GSM3199267 !Series_sample_id = GSM3199268 !Series_sample_id = GSM3199269 !Series_sample_id = GSM3199270 !Series_sample_id = GSM3199271 !Series_sample_id = GSM3199272 !Series_sample_id = GSM3199273 !Series_sample_id = GSM3199274 !Series_sample_id = GSM3199275 !Series_sample_id = GSM3199276 !Series_sample_id = GSM3199277 !Series_sample_id = GSM3199278 !Series_sample_id = GSM3199279 !Series_sample_id = GSM3199280 !Series_sample_id = GSM3199281 !Series_sample_id = GSM3199282 !Series_sample_id = GSM3199283 !Series_sample_id = GSM3199284 !Series_sample_id = GSM3199285 !Series_sample_id = GSM3199286 !Series_sample_id = GSM3199287 !Series_sample_id = GSM3199288 !Series_sample_id = GSM3199289 !Series_sample_id = GSM3199290 !Series_sample_id = GSM3199291 !Series_sample_id = GSM3199292 !Series_sample_id = GSM3199293 !Series_sample_id = GSM3199294 !Series_sample_id = GSM3199295 !Series_sample_id = GSM3199296 !Series_sample_id = GSM3199297 !Series_sample_id = GSM3199298 !Series_sample_id = GSM3199299 !Series_sample_id = GSM3199300 !Series_sample_id = GSM3199301 !Series_sample_id = GSM3199302 !Series_sample_id = GSM3199303 !Series_sample_id = GSM3199304 !Series_sample_id = GSM3199305 !Series_sample_id = GSM3199306 !Series_sample_id = GSM3199307 !Series_sample_id = GSM3199308 !Series_sample_id = GSM3199309 !Series_sample_id = GSM3199310 !Series_sample_id = GSM3199311 !Series_sample_id = GSM3199312 !Series_sample_id = GSM3199313 !Series_sample_id = GSM3199314 !Series_sample_id = GSM3199315 !Series_sample_id = GSM3199316 !Series_sample_id = GSM3199317 !Series_sample_id = GSM3199318 !Series_sample_id = GSM3199319 !Series_sample_id = GSM3199320 !Series_sample_id = GSM3199321 !Series_sample_id = GSM3199322 !Series_sample_id = GSM3199323 !Series_sample_id = GSM3199324 !Series_sample_id = GSM3199325 !Series_sample_id = GSM3199326 !Series_sample_id = GSM3199327 !Series_sample_id = GSM3199328 !Series_sample_id = GSM3199329 !Series_sample_id = GSM3199330 !Series_sample_id = GSM3199331 !Series_sample_id = GSM3199332 !Series_sample_id = GSM3199333 !Series_sample_id = GSM3199334 !Series_sample_id = GSM3199335 !Series_sample_id = GSM3199336 !Series_sample_id = GSM3199337 !Series_sample_id = GSM3199338 !Series_sample_id = GSM3199339 !Series_sample_id = GSM3199340 !Series_sample_id = GSM3199341 !Series_sample_id = GSM3199342 !Series_sample_id = GSM3199343 !Series_sample_id = GSM3199344 !Series_sample_id = GSM3199345 !Series_sample_id = GSM3199346 !Series_sample_id = GSM3199347 !Series_sample_id = GSM3199348 !Series_sample_id = GSM3199349 !Series_sample_id = GSM3199350 !Series_sample_id = GSM3199351 !Series_sample_id = GSM3199352 !Series_sample_id = GSM3199353 !Series_sample_id = GSM3199354 !Series_sample_id = GSM3199355 !Series_sample_id = GSM3199356 !Series_sample_id = GSM3199357 !Series_sample_id = GSM3199358 !Series_sample_id = GSM3199359 !Series_sample_id = GSM3199360 !Series_sample_id = GSM3199361 !Series_sample_id = GSM3199362 !Series_sample_id = GSM3199363 !Series_sample_id = GSM3199364 !Series_sample_id = GSM3199365 !Series_sample_id = GSM3199366 !Series_sample_id = GSM3199367 !Series_sample_id = GSM3199368 !Series_sample_id = GSM3199369 !Series_sample_id = GSM3199370 !Series_sample_id = GSM3199371 !Series_sample_id = GSM3199372 !Series_sample_id = GSM3199373 !Series_sample_id = GSM3199374 !Series_sample_id = GSM3199375 !Series_sample_id = GSM3199376 !Series_sample_id = GSM3199377 !Series_sample_id = GSM3199378 !Series_sample_id = GSM3199379 !Series_sample_id = GSM3199380 !Series_sample_id = GSM3199381 !Series_sample_id = GSM3199382 !Series_sample_id = GSM3199383 !Series_sample_id = GSM3199384 !Series_sample_id = GSM3199385 !Series_sample_id = GSM3199386 !Series_sample_id = GSM3199387 !Series_sample_id = GSM3199388 !Series_sample_id = GSM3199389 !Series_sample_id = GSM3199390 !Series_sample_id = GSM3199391 !Series_sample_id = GSM3199392 !Series_sample_id = GSM3199393 !Series_sample_id = GSM3199394 !Series_sample_id = GSM3199395 !Series_sample_id = GSM3199396 !Series_sample_id = GSM3199397 !Series_sample_id = GSM3199398 !Series_sample_id = GSM3199399 !Series_sample_id = GSM3199400 !Series_sample_id = GSM3199401 !Series_sample_id = GSM3199402 !Series_sample_id = GSM3199403 !Series_sample_id = GSM3199404 !Series_sample_id = GSM3199405 !Series_sample_id = GSM3199406 !Series_sample_id = GSM3199407 !Series_sample_id = GSM3199408 !Series_sample_id = GSM3199409 !Series_sample_id = GSM3199410 !Series_sample_id = GSM3199411 !Series_sample_id = GSM3199412 !Series_sample_id = GSM3199413 !Series_sample_id = GSM3199414 !Series_sample_id = GSM3199415 !Series_sample_id = GSM3199416 !Series_sample_id = GSM3199417 !Series_sample_id = GSM3199418 !Series_sample_id = GSM3199419 !Series_sample_id = GSM3199420 !Series_sample_id = GSM3199421 !Series_sample_id = GSM3199422 !Series_sample_id = GSM3199423 !Series_sample_id = GSM3199424 !Series_sample_id = GSM3199425 !Series_sample_id = GSM3199426 !Series_sample_id = GSM3199427 !Series_sample_id = GSM3199428 !Series_sample_id = GSM3199429 !Series_sample_id = GSM3199430 !Series_sample_id = GSM3199431 !Series_sample_id = GSM3199432 !Series_sample_id = GSM3199433 !Series_sample_id = GSM3199434 !Series_sample_id = GSM3199435 !Series_sample_id = GSM3199436 !Series_sample_id = GSM3199437 !Series_sample_id = GSM3199438 !Series_sample_id = GSM3199439 !Series_sample_id = GSM3199440 !Series_sample_id = GSM3199441 !Series_sample_id = GSM3199442 !Series_sample_id = GSM3199443 !Series_sample_id = GSM3199444 !Series_sample_id = GSM3199445 !Series_sample_id = GSM3199446 !Series_sample_id = GSM3199447 !Series_sample_id = GSM3199448 !Series_sample_id = GSM3199449 !Series_sample_id = GSM3199450 !Series_sample_id = GSM3199451 !Series_sample_id = GSM3199452 !Series_sample_id = GSM3199453 !Series_sample_id = GSM3199454 !Series_sample_id = GSM3199455 !Series_sample_id = GSM3199456 !Series_sample_id = GSM3199457 !Series_sample_id = GSM3199458 !Series_sample_id = GSM3199459 !Series_sample_id = GSM3199460 !Series_sample_id = GSM3199461 !Series_sample_id = GSM3199462 !Series_sample_id = GSM3199463 !Series_sample_id = GSM3199464 !Series_sample_id = GSM3199465 !Series_sample_id = GSM3199466 !Series_sample_id = GSM3199467 !Series_sample_id = GSM3199468 !Series_sample_id = GSM3199469 !Series_sample_id = GSM3199470 !Series_sample_id = GSM3199471 !Series_sample_id = GSM3199472 !Series_sample_id = GSM3199473 !Series_sample_id = GSM3199474 !Series_sample_id = GSM3199475 !Series_sample_id = GSM3199476 !Series_sample_id = GSM3199477 !Series_sample_id = GSM3199478 !Series_sample_id = GSM3199479 !Series_sample_id = GSM3199480 !Series_sample_id = GSM3199481 !Series_sample_id = GSM3199482 !Series_sample_id = GSM3199483 !Series_sample_id = GSM3199484 !Series_sample_id = GSM3199485 !Series_sample_id = GSM3199486 !Series_sample_id = GSM3199487 !Series_sample_id = GSM3199488 !Series_sample_id = GSM3199489 !Series_sample_id = GSM3199490 !Series_sample_id = GSM3199491 !Series_sample_id = GSM3199492 !Series_sample_id = GSM3199493 !Series_sample_id = GSM3199494 !Series_sample_id = 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GSM3200007 !Series_sample_id = GSM3200008 !Series_sample_id = GSM3200009 !Series_sample_id = GSM3200010 !Series_sample_id = GSM3200011 !Series_sample_id = GSM3200012 !Series_sample_id = GSM3200013 !Series_sample_id = GSM3200014 !Series_sample_id = GSM3200015 !Series_sample_id = GSM3200016 !Series_sample_id = GSM3200017 !Series_sample_id = GSM3200018 !Series_sample_id = GSM3200019 !Series_sample_id = GSM3200020 !Series_sample_id = GSM3200021 !Series_sample_id = GSM3200022 !Series_sample_id = GSM3200023 !Series_sample_id = GSM3200024 !Series_sample_id = GSM3200025 !Series_sample_id = GSM3200026 !Series_sample_id = GSM3200027 !Series_sample_id = GSM3200028 !Series_sample_id = GSM3200029 !Series_sample_id = GSM3200030 !Series_sample_id = GSM3200031 !Series_sample_id = GSM3200032 !Series_sample_id = GSM3200033 !Series_sample_id = GSM3200034 !Series_sample_id = GSM3200035 !Series_sample_id = GSM3200036 !Series_sample_id = GSM3200037 !Series_sample_id = GSM3200038 !Series_sample_id = GSM3200039 !Series_sample_id = GSM3200040 !Series_sample_id = GSM3200041 !Series_sample_id = GSM3200042 !Series_sample_id = GSM3200043 !Series_sample_id = GSM3200044 !Series_sample_id = GSM3200045 !Series_sample_id = GSM3200046 !Series_sample_id = GSM3200047 !Series_sample_id = GSM3200048 !Series_sample_id = GSM3200049 !Series_sample_id = GSM3200050 !Series_sample_id = GSM3200051 !Series_sample_id = GSM3200052 !Series_sample_id = GSM3200053 !Series_sample_id = GSM3200054 !Series_sample_id = GSM3200055 !Series_sample_id = GSM3200056 !Series_sample_id = GSM3200057 !Series_sample_id = GSM3200058 !Series_sample_id = GSM3200059 !Series_sample_id = GSM3200060 !Series_sample_id = GSM3200061 !Series_sample_id = GSM3200062 !Series_sample_id = GSM3200063 !Series_sample_id = GSM3200064 !Series_sample_id = GSM3200065 !Series_sample_id = GSM3200066 !Series_sample_id = GSM3200067 !Series_sample_id = GSM3200068 !Series_sample_id = GSM3200069 !Series_sample_id = GSM3200070 !Series_sample_id = GSM3200071 !Series_sample_id = GSM3200072 !Series_sample_id = GSM3200073 !Series_sample_id = GSM3200074 !Series_sample_id = GSM3200075 !Series_sample_id = GSM3200076 !Series_sample_id = GSM3200077 !Series_sample_id = GSM3200078 !Series_sample_id = GSM3200079 !Series_sample_id = GSM3200080 !Series_sample_id = GSM3200081 !Series_sample_id = GSM3200082 !Series_sample_id = GSM3200083 !Series_sample_id = GSM3200084 !Series_sample_id = GSM3200085 !Series_sample_id = GSM3200086 !Series_sample_id = GSM3200087 !Series_sample_id = GSM3200088 !Series_sample_id = GSM3200089 !Series_sample_id = GSM3200090 !Series_sample_id = GSM3200091 !Series_sample_id = GSM3200092 !Series_sample_id = GSM3200093 !Series_sample_id = GSM3200094 !Series_sample_id = GSM3200095 !Series_sample_id = GSM3200096 !Series_sample_id = GSM3200097 !Series_sample_id = GSM3200098 !Series_sample_id = GSM3200099 !Series_sample_id = GSM3200100 !Series_sample_id = GSM3200101 !Series_sample_id = GSM3200102 !Series_sample_id = GSM3200103 !Series_sample_id = GSM3200104 !Series_sample_id = GSM3200105 !Series_sample_id = GSM3200106 !Series_sample_id = GSM3200107 !Series_sample_id = GSM3200108 !Series_sample_id = GSM3200109 !Series_sample_id = GSM3200110 !Series_sample_id = GSM3200111 !Series_sample_id = GSM3200112 !Series_sample_id = GSM3200113 !Series_sample_id = GSM3200114 !Series_sample_id = GSM3200115 !Series_sample_id = GSM3200116 !Series_sample_id = GSM3200117 !Series_sample_id = GSM3200118 !Series_sample_id = GSM3200119 !Series_sample_id = GSM3200120 !Series_sample_id = GSM3200121 !Series_sample_id = GSM3200122 !Series_sample_id = GSM3200123 !Series_sample_id = GSM3200124 !Series_sample_id = GSM3200125 !Series_sample_id = GSM3200126 !Series_sample_id = GSM3200127 !Series_sample_id = GSM3200128 !Series_sample_id = GSM3200129 !Series_sample_id = GSM3200130 !Series_sample_id = GSM3200131 !Series_sample_id = GSM3200132 !Series_sample_id = GSM3200133 !Series_sample_id = GSM3200134 !Series_sample_id = GSM3200135 !Series_sample_id = GSM3200136 !Series_sample_id = GSM3200137 !Series_sample_id = GSM3200138 !Series_sample_id = GSM3200139 !Series_sample_id = GSM3200140 !Series_sample_id = GSM3200141 !Series_sample_id = GSM3200142 !Series_sample_id = GSM3200143 !Series_sample_id = GSM3200144 !Series_sample_id = GSM3200145 !Series_sample_id = GSM3200146 !Series_sample_id = GSM3200147 !Series_sample_id = GSM3200148 !Series_sample_id = GSM3200149 !Series_sample_id = GSM3200150 !Series_sample_id = GSM3200151 !Series_sample_id = GSM3200152 !Series_sample_id = GSM3200153 !Series_sample_id = GSM3200154 !Series_sample_id = GSM3200155 !Series_sample_id = GSM3200156 !Series_sample_id = GSM3200157 !Series_sample_id = GSM3200158 !Series_sample_id = GSM3200159 !Series_sample_id = GSM3200160 !Series_sample_id = GSM3200161 !Series_sample_id = GSM3200162 !Series_sample_id = GSM3200163 !Series_sample_id = GSM3200164 !Series_sample_id = GSM3200165 !Series_sample_id = GSM3200166 !Series_sample_id = GSM3200167 !Series_sample_id = GSM3200168 !Series_sample_id = GSM3200169 !Series_sample_id = GSM3200170 !Series_sample_id = GSM3200171 !Series_sample_id = GSM3200172 !Series_sample_id = GSM3200173 !Series_sample_id = GSM3200174 !Series_sample_id = GSM3200175 !Series_sample_id = GSM3200176 !Series_sample_id = GSM3200177 !Series_sample_id = GSM3200178 !Series_sample_id = GSM3200179 !Series_sample_id = GSM3200180 !Series_sample_id = GSM3200181 !Series_sample_id = GSM3200182 !Series_sample_id = GSM3200183 !Series_sample_id = GSM3200184 !Series_sample_id = GSM3200185 !Series_sample_id = GSM3200186 !Series_sample_id = GSM3200187 !Series_sample_id = GSM3200188 !Series_sample_id = GSM3200189 !Series_sample_id = GSM3200190 !Series_sample_id = GSM3200191 !Series_sample_id = GSM3200192 !Series_sample_id = GSM3200193 !Series_sample_id = GSM3200194 !Series_sample_id = GSM3200195 !Series_sample_id = GSM3200196 !Series_sample_id = GSM3200197 !Series_sample_id = GSM3200198 !Series_sample_id = GSM3200199 !Series_sample_id = GSM3200200 !Series_sample_id = GSM3200201 !Series_sample_id = GSM3200202 !Series_sample_id = GSM3200203 !Series_sample_id = GSM3200204 !Series_sample_id = GSM3200205 !Series_sample_id = GSM3200206 !Series_sample_id = GSM3200207 !Series_sample_id = GSM3200208 !Series_sample_id = GSM3200209 !Series_sample_id = GSM3200210 !Series_sample_id = GSM3200211 !Series_sample_id = GSM3200212 !Series_sample_id = GSM3200213 !Series_sample_id = GSM3200214 !Series_sample_id = GSM3200215 !Series_sample_id = GSM3200216 !Series_sample_id = GSM3200217 !Series_sample_id = GSM3200218 !Series_sample_id = GSM3200219 !Series_sample_id = GSM3200220 !Series_sample_id = GSM3200221 !Series_sample_id = GSM3200222 !Series_sample_id = GSM3200223 !Series_sample_id = GSM3200224 !Series_sample_id = GSM3200225 !Series_sample_id = GSM3200226 !Series_sample_id = GSM3200227 !Series_sample_id = GSM3200228 !Series_sample_id = GSM3200229 !Series_sample_id = GSM3200230 !Series_sample_id = GSM3200231 !Series_sample_id = GSM3200232 !Series_sample_id = GSM3200233 !Series_sample_id = GSM3200234 !Series_sample_id = GSM3200235 !Series_sample_id = GSM3200236 !Series_sample_id = GSM3200237 !Series_sample_id = GSM3200238 !Series_sample_id = GSM3200239 !Series_sample_id = GSM3200240 !Series_sample_id = GSM3200241 !Series_sample_id = GSM3200242 !Series_sample_id = GSM3200243 !Series_sample_id = GSM3200244 !Series_sample_id = GSM3200245 !Series_sample_id = GSM3200246 !Series_sample_id = GSM3200247 !Series_sample_id = GSM3200248 !Series_sample_id = GSM3200249 !Series_sample_id = GSM3200250 !Series_sample_id = GSM3200251 !Series_sample_id = GSM3200252 !Series_sample_id = GSM3200253 !Series_sample_id = GSM3200254 !Series_sample_id = GSM3200255 !Series_sample_id = GSM3200256 !Series_sample_id = GSM3200257 !Series_sample_id = GSM3200258 !Series_sample_id = GSM3200259 !Series_sample_id = GSM3200260 !Series_sample_id = GSM3200261 !Series_sample_id = GSM3200262 !Series_sample_id = GSM3200263 !Series_sample_id = GSM3200264 !Series_sample_id = GSM3200265 !Series_sample_id = GSM3200266 !Series_sample_id = GSM3200267 !Series_sample_id = GSM3200268 !Series_sample_id = GSM3200269 !Series_sample_id = GSM3200270 !Series_sample_id = GSM3200271 !Series_sample_id = GSM3200272 !Series_sample_id = GSM3200273 !Series_sample_id = GSM3200274 !Series_sample_id = GSM3200275 !Series_sample_id = GSM3200276 !Series_sample_id = GSM3200277 !Series_sample_id = GSM3200278 !Series_sample_id = GSM3200279 !Series_sample_id = GSM3200280 !Series_sample_id = GSM3200281 !Series_sample_id = GSM3200282 !Series_sample_id = GSM3200283 !Series_sample_id = GSM3200284 !Series_sample_id = GSM3200285 !Series_sample_id = GSM3200286 !Series_sample_id = GSM3200287 !Series_sample_id = GSM3200288 !Series_sample_id = GSM3200289 !Series_sample_id = GSM3200290 !Series_sample_id = GSM3200291 !Series_sample_id = GSM3200292 !Series_sample_id = GSM3200293 !Series_sample_id = GSM3200294 !Series_sample_id = GSM3200295 !Series_sample_id = GSM3200296 !Series_sample_id = GSM3200297 !Series_sample_id = GSM3200298 !Series_sample_id = GSM3200299 !Series_sample_id = GSM3200300 !Series_sample_id = GSM3200301 !Series_sample_id = GSM3200302 !Series_sample_id = GSM3200303 !Series_sample_id = GSM3200304 !Series_sample_id = GSM3200305 !Series_sample_id = GSM3200306 !Series_sample_id = GSM3200307 !Series_sample_id = GSM3200308 !Series_sample_id = GSM3200309 !Series_sample_id = GSM3200310 !Series_sample_id = GSM3200311 !Series_sample_id = GSM3200312 !Series_sample_id = GSM3200313 !Series_sample_id = GSM3200314 !Series_sample_id = GSM3200315 !Series_sample_id = GSM3200316 !Series_sample_id = GSM3200317 !Series_sample_id = GSM3200318 !Series_sample_id = GSM3200319 !Series_sample_id = GSM3200320 !Series_sample_id = GSM3200321 !Series_sample_id = GSM3200322 !Series_sample_id = GSM3200323 !Series_sample_id = GSM3200324 !Series_sample_id = GSM3200325 !Series_sample_id = GSM3200326 !Series_sample_id = GSM3200327 !Series_sample_id = GSM3200328 !Series_sample_id = GSM3200329 !Series_sample_id = GSM3200330 !Series_sample_id = GSM3200331 !Series_sample_id = GSM3200332 !Series_sample_id = GSM3200333 !Series_sample_id = GSM3200334 !Series_sample_id = GSM3200335 !Series_sample_id = GSM3200336 !Series_sample_id = GSM3200337 !Series_sample_id = GSM3200338 !Series_sample_id = GSM3200339 !Series_sample_id = GSM3200340 !Series_sample_id = GSM3200341 !Series_sample_id = GSM3200342 !Series_sample_id = GSM3200343 !Series_sample_id = GSM3200344 !Series_sample_id = GSM3200345 !Series_sample_id = GSM3200346 !Series_sample_id = GSM3200347 !Series_sample_id = GSM3200348 !Series_sample_id = GSM3200349 !Series_sample_id = GSM3200350 !Series_sample_id = GSM3200351 !Series_sample_id = GSM3200352 !Series_sample_id = GSM3200353 !Series_sample_id = GSM3200354 !Series_sample_id = GSM3200355 !Series_sample_id = GSM3200356 !Series_sample_id = GSM3200357 !Series_sample_id = GSM3200358 !Series_sample_id = GSM3200359 !Series_sample_id = GSM3200360 !Series_sample_id = GSM3200361 !Series_sample_id = GSM3200362 !Series_sample_id = GSM3200363 !Series_sample_id = GSM3200364 !Series_sample_id = GSM3200365 !Series_sample_id = GSM3200366 !Series_sample_id = GSM3200367 !Series_sample_id = GSM3200368 !Series_sample_id = GSM3200369 !Series_sample_id = GSM3200370 !Series_sample_id = GSM3200371 !Series_sample_id = GSM3200372 !Series_sample_id = GSM3200373 !Series_sample_id = GSM3200374 !Series_sample_id = GSM3200375 !Series_sample_id = GSM3200376 !Series_sample_id = GSM3200377 !Series_sample_id = GSM3200378 !Series_sample_id = GSM3200379 !Series_sample_id = GSM3200380 !Series_sample_id = GSM3200381 !Series_sample_id = GSM3200382 !Series_sample_id = GSM3200383 !Series_sample_id = GSM3200384 !Series_sample_id = GSM3200385 !Series_sample_id = GSM3200386 !Series_sample_id = GSM3200387 !Series_sample_id = GSM3200388 !Series_sample_id = GSM3200389 !Series_sample_id = GSM3200390 !Series_sample_id = GSM3200391 !Series_sample_id = GSM3200392 !Series_sample_id = GSM3200393 !Series_sample_id = GSM3200394 !Series_sample_id = GSM3200395 !Series_sample_id = GSM3200396 !Series_sample_id = GSM3200397 !Series_sample_id = GSM3200398 !Series_sample_id = GSM3200399 !Series_sample_id = GSM3200400 !Series_sample_id = GSM3200401 !Series_sample_id = GSM3200402 !Series_sample_id = GSM3200403 !Series_sample_id = GSM3200404 !Series_sample_id = GSM3200405 !Series_sample_id = GSM3200406 !Series_sample_id = GSM3200407 !Series_sample_id = GSM3200408 !Series_sample_id = GSM3200409 !Series_sample_id = GSM3200410 !Series_sample_id = GSM3200411 !Series_sample_id = GSM3200412 !Series_sample_id = GSM3200413 !Series_sample_id = GSM3200414 !Series_sample_id = GSM3200415 !Series_sample_id = GSM3200416 !Series_sample_id = GSM3200417 !Series_sample_id = GSM3200418 !Series_sample_id = GSM3200419 !Series_sample_id = GSM3200420 !Series_sample_id = GSM3200421 !Series_sample_id = GSM3200422 !Series_sample_id = GSM3200423 !Series_sample_id = GSM3200424 !Series_sample_id = GSM3200425 !Series_sample_id = GSM3200426 !Series_sample_id = GSM3200427 !Series_sample_id = GSM3200428 !Series_sample_id = GSM3200429 !Series_sample_id = GSM3200430 !Series_sample_id = GSM3200431 !Series_sample_id = GSM3200432 !Series_sample_id = GSM3200433 !Series_sample_id = GSM3200434 !Series_sample_id = GSM3200435 !Series_sample_id = GSM3200436 !Series_sample_id = GSM3200437 !Series_sample_id = GSM3200438 !Series_sample_id = GSM3200439 !Series_sample_id = GSM3200440 !Series_sample_id = GSM3200441 !Series_sample_id = GSM3200442 !Series_sample_id = GSM3200443 !Series_sample_id = GSM3200444 !Series_sample_id = GSM3200445 !Series_sample_id = GSM3200446 !Series_sample_id = GSM3200447 !Series_sample_id = GSM3200448 !Series_sample_id = GSM3200449 !Series_sample_id = GSM3200450 !Series_sample_id = GSM3200451 !Series_sample_id = GSM3200452 !Series_sample_id = GSM3200453 !Series_sample_id = GSM3200454 !Series_sample_id = GSM3200455 !Series_sample_id = GSM3200456 !Series_sample_id = GSM3200457 !Series_sample_id = GSM3200458 !Series_sample_id = GSM3200459 !Series_sample_id = GSM3200460 !Series_sample_id = GSM3200461 !Series_sample_id = GSM3200462 !Series_sample_id = GSM3200463 !Series_sample_id = GSM3200464 !Series_sample_id = GSM3200465 !Series_sample_id = GSM3200466 !Series_sample_id = GSM3200467 !Series_sample_id = GSM3200468 !Series_sample_id = GSM3200469 !Series_sample_id = GSM3200470 !Series_sample_id = GSM3200471 !Series_sample_id = GSM3200472 !Series_sample_id = GSM3200473 !Series_sample_id = GSM3200474 !Series_sample_id = GSM3200475 !Series_sample_id = GSM3200476 !Series_sample_id = GSM3200477 !Series_sample_id = GSM3200478 !Series_sample_id = GSM3200479 !Series_sample_id = GSM3200480 !Series_sample_id = GSM3200481 !Series_sample_id = GSM3200482 !Series_sample_id = GSM3200483 !Series_sample_id = GSM3200484 !Series_sample_id = GSM3200485 !Series_sample_id = GSM3200486 !Series_sample_id = GSM3200487 !Series_sample_id = GSM3200488 !Series_sample_id = GSM3200489 !Series_sample_id = GSM3200490 !Series_sample_id = GSM3200491 !Series_sample_id = GSM3200492 !Series_sample_id = GSM3200493 !Series_sample_id = GSM3200494 !Series_sample_id = GSM3200495 !Series_sample_id = GSM3200496 !Series_sample_id = GSM3200497 !Series_sample_id = GSM3200498 !Series_sample_id = GSM3200499 !Series_sample_id = GSM3200500 !Series_sample_id = GSM3200501 !Series_sample_id = GSM3200502 !Series_sample_id = GSM3200503 !Series_sample_id = GSM3200504 !Series_sample_id = GSM3200505 !Series_sample_id = GSM3200506 !Series_sample_id = GSM3200507 !Series_sample_id = GSM3200508 !Series_sample_id = GSM3200509 !Series_sample_id = GSM3200510 !Series_sample_id = GSM3200511 !Series_sample_id = GSM3200512 !Series_sample_id = GSM3200513 !Series_sample_id = GSM3200514 !Series_sample_id = GSM3200515 !Series_sample_id = GSM3200516 !Series_sample_id = GSM3200517 !Series_sample_id = GSM3200518 !Series_sample_id = GSM3200519 !Series_sample_id = GSM3200520 !Series_sample_id = GSM3200521 !Series_sample_id = GSM3200522 !Series_sample_id = GSM3200523 !Series_sample_id = GSM3200524 !Series_sample_id = GSM3200525 !Series_sample_id = GSM3200526 !Series_sample_id = GSM3200527 !Series_sample_id = GSM3200528 !Series_sample_id = GSM3200529 !Series_sample_id = GSM3200530 !Series_sample_id = GSM3200531 !Series_sample_id = GSM3200532 !Series_sample_id = GSM3200533 !Series_sample_id = GSM3200534 !Series_sample_id = GSM3200535 !Series_sample_id = GSM3200536 !Series_sample_id = GSM3200537 !Series_sample_id = GSM3200538 !Series_sample_id = GSM3200539 !Series_sample_id = GSM3200540 !Series_sample_id = GSM3200541 !Series_sample_id = GSM3200542 !Series_sample_id = GSM3200543 !Series_sample_id = GSM3200544 !Series_sample_id = GSM3200545 !Series_sample_id = GSM3200546 !Series_sample_id = GSM3200547 !Series_sample_id = GSM3200548 !Series_sample_id = GSM3200549 !Series_sample_id = GSM3200550 !Series_sample_id = GSM3200551 !Series_sample_id = GSM3200552 !Series_sample_id = GSM3200553 !Series_sample_id = GSM3200554 !Series_sample_id = GSM3200555 !Series_sample_id = GSM3200556 !Series_sample_id = GSM3200557 !Series_sample_id = GSM3200558 !Series_sample_id = GSM3200559 !Series_sample_id = GSM3200560 !Series_sample_id = GSM3200561 !Series_sample_id = GSM3200562 !Series_sample_id = GSM3200563 !Series_sample_id = GSM3200564 !Series_sample_id = GSM3200565 !Series_sample_id = GSM3200566 !Series_sample_id = GSM3200567 !Series_sample_id = GSM3200568 !Series_sample_id = GSM3200569 !Series_sample_id = GSM3200570 !Series_sample_id = GSM3200571 !Series_sample_id = GSM3200572 !Series_sample_id = GSM3200573 !Series_sample_id = GSM3200574 !Series_sample_id = GSM3200575 !Series_sample_id = GSM3200576 !Series_sample_id = GSM3200577 !Series_sample_id = GSM3200578 !Series_sample_id = GSM3200579 !Series_sample_id = GSM3200580 !Series_sample_id = GSM3200581 !Series_sample_id = GSM3200582 !Series_sample_id = GSM3200583 !Series_sample_id = GSM3200584 !Series_sample_id = GSM3200585 !Series_sample_id = GSM3200586 !Series_sample_id = GSM3200587 !Series_sample_id = GSM3200588 !Series_sample_id = GSM3200589 !Series_sample_id = GSM3200590 !Series_sample_id = GSM3200591 !Series_sample_id = GSM3200592 !Series_sample_id = GSM3200593 !Series_sample_id = GSM3200594 !Series_sample_id = GSM3200595 !Series_sample_id = GSM3200596 !Series_sample_id = GSM3200597 !Series_sample_id = GSM3200598 !Series_sample_id = GSM3200599 !Series_sample_id = GSM3200600 !Series_sample_id = GSM3200601 !Series_sample_id = GSM3200602 !Series_sample_id = GSM3200603 !Series_sample_id = GSM3200604 !Series_sample_id = GSM3200605 !Series_sample_id = GSM3200606 !Series_sample_id = GSM3200607 !Series_sample_id = GSM3200608 !Series_sample_id = GSM3200609 !Series_sample_id = GSM3200610 !Series_sample_id = GSM3200611 !Series_sample_id = GSM3200612 !Series_sample_id = GSM3200613 !Series_sample_id = GSM3200614 !Series_sample_id = GSM3200615 !Series_sample_id = GSM3200616 !Series_sample_id = GSM3200617 !Series_sample_id = GSM3200618 !Series_sample_id = GSM3200619 !Series_sample_id = GSM3200620 !Series_sample_id = GSM3200621 !Series_sample_id = GSM3200622 !Series_sample_id = GSM3200623 !Series_sample_id = GSM3200624 !Series_sample_id = GSM3200625 !Series_sample_id = GSM3200626 !Series_sample_id = GSM3200627 !Series_sample_id = GSM3200628 !Series_sample_id = GSM3200629 !Series_sample_id = GSM3200630 !Series_sample_id = GSM3200631 !Series_sample_id = GSM3200632 !Series_sample_id = GSM3200633 !Series_sample_id = GSM3200634 !Series_sample_id = GSM3200635 !Series_sample_id = GSM3200636 !Series_sample_id = GSM3200637 !Series_sample_id = GSM3200638 !Series_sample_id = GSM3200639 !Series_sample_id = GSM3200640 !Series_sample_id = GSM3200641 !Series_sample_id = GSM3200642 !Series_sample_id = GSM3200643 !Series_sample_id = GSM3200644 !Series_sample_id = GSM3200645 !Series_sample_id = GSM3200646 !Series_sample_id = GSM3200647 !Series_sample_id = GSM3200648 !Series_sample_id = GSM3200649 !Series_sample_id = GSM3200650 !Series_sample_id = GSM3200651 !Series_sample_id = GSM3200652 !Series_sample_id = GSM3200653 !Series_sample_id = GSM3200654 !Series_sample_id = GSM3200655 !Series_sample_id = GSM3200656 !Series_sample_id = GSM3200657 !Series_sample_id = GSM3200658 !Series_sample_id = GSM3200659 !Series_sample_id = GSM3200660 !Series_sample_id = GSM3200661 !Series_sample_id = GSM3200662 !Series_sample_id = GSM3200663 !Series_sample_id = GSM3200664 !Series_sample_id = GSM3200665 !Series_sample_id = GSM3200666 !Series_sample_id = GSM3200667 !Series_sample_id = GSM3200668 !Series_sample_id = GSM3200669 !Series_sample_id = GSM3200670 !Series_sample_id = GSM3200671 !Series_sample_id = GSM3200672 !Series_sample_id = GSM3200673 !Series_sample_id = GSM3200674 !Series_sample_id = GSM3200675 !Series_sample_id = GSM3200676 !Series_sample_id = GSM3200677 !Series_sample_id = GSM3200678 !Series_sample_id = GSM3200679 !Series_sample_id = GSM3200680 !Series_sample_id = GSM3200681 !Series_sample_id = GSM3200682 !Series_sample_id = GSM3200683 !Series_sample_id = GSM3200684 !Series_sample_id = GSM3200685 !Series_sample_id = GSM3200686 !Series_sample_id = GSM3200687 !Series_sample_id = GSM3200688 !Series_sample_id = GSM3200689 !Series_sample_id = GSM3200690 !Series_sample_id = GSM3200691 !Series_sample_id = GSM3200692 !Series_sample_id = GSM3200693 !Series_sample_id = GSM3200694 !Series_sample_id = GSM3200695 !Series_sample_id = GSM3200696 !Series_sample_id = GSM3200697 !Series_sample_id = GSM3200698 !Series_sample_id = GSM3200699 !Series_sample_id = GSM3200700 !Series_sample_id = GSM3200701 !Series_sample_id = GSM3200702 !Series_sample_id = GSM3200703 !Series_sample_id = GSM3200704 !Series_sample_id = GSM3200705 !Series_sample_id = GSM3200706 !Series_sample_id = GSM3200707 !Series_sample_id = GSM3200708 !Series_sample_id = GSM3200709 !Series_sample_id = GSM3200710 !Series_sample_id = GSM3200711 !Series_sample_id = GSM3200712 !Series_sample_id = GSM3200713 !Series_sample_id = GSM3200714 !Series_sample_id = GSM3200715 !Series_sample_id = GSM3200716 !Series_sample_id = GSM3200717 !Series_sample_id = GSM3200718 !Series_sample_id = GSM3200719 !Series_sample_id = GSM3200720 !Series_sample_id = GSM3200721 !Series_sample_id = GSM3200722 !Series_sample_id = GSM3200723 !Series_sample_id = GSM3200724 !Series_sample_id = GSM3200725 !Series_sample_id = GSM3200726 !Series_sample_id = GSM3200727 !Series_sample_id = GSM3200728 !Series_sample_id = GSM3200729 !Series_sample_id = GSM3200730 !Series_sample_id = GSM3200731 !Series_sample_id = GSM3200732 !Series_sample_id = GSM3200733 !Series_sample_id = GSM3200734 !Series_sample_id = GSM3200735 !Series_sample_id = GSM3200736 !Series_sample_id = GSM3200737 !Series_sample_id = GSM3200738 !Series_sample_id = GSM3200739 !Series_sample_id = GSM3200740 !Series_sample_id = GSM3200741 !Series_sample_id = GSM3200742 !Series_sample_id = GSM3200743 !Series_sample_id = GSM3200744 !Series_sample_id = GSM3200745 !Series_sample_id = GSM3200746 !Series_sample_id = GSM3200747 !Series_sample_id = GSM3200748 !Series_sample_id = GSM3200749 !Series_sample_id = GSM3200750 !Series_sample_id = GSM3200751 !Series_sample_id = GSM3200752 !Series_sample_id = GSM3200753 !Series_sample_id = GSM3200754 !Series_sample_id = GSM3200755 !Series_sample_id = GSM3200756 !Series_sample_id = GSM3200757 !Series_sample_id = GSM3200758 !Series_sample_id = GSM3200759 !Series_sample_id = GSM3200760 !Series_sample_id = GSM3200761 !Series_sample_id = GSM3200762 !Series_sample_id = GSM3200763 !Series_sample_id = GSM3200764 !Series_sample_id = GSM3200765 !Series_sample_id = GSM3200766 !Series_sample_id = GSM3200767 !Series_sample_id = GSM3200768 !Series_sample_id = GSM3200769 !Series_sample_id = GSM3200770 !Series_sample_id = GSM3200771 !Series_sample_id = GSM3200772 !Series_sample_id = GSM3200773 !Series_sample_id = GSM3200774 !Series_sample_id = GSM3200775 !Series_sample_id = GSM3200776 !Series_sample_id = GSM3200777 !Series_sample_id = GSM3200778 !Series_sample_id = GSM3200779 !Series_sample_id = GSM3200780 !Series_sample_id = GSM3200781 !Series_sample_id = GSM3200782 !Series_sample_id = GSM3200783 !Series_sample_id = GSM3200784 !Series_sample_id = GSM3200785 !Series_sample_id = GSM3200786 !Series_sample_id = GSM3200787 !Series_sample_id = GSM3200788 !Series_sample_id = GSM3200789 !Series_sample_id = GSM3200790 !Series_sample_id = GSM3200791 !Series_sample_id = GSM3200792 !Series_sample_id = GSM3200793 !Series_sample_id = GSM3200794 !Series_sample_id = GSM3200795 !Series_sample_id = GSM3200796 !Series_sample_id = GSM3200797 !Series_sample_id = GSM3200798 !Series_sample_id = GSM3200799 !Series_sample_id = GSM3200800 !Series_sample_id = GSM3200801 !Series_sample_id = GSM3200802 !Series_sample_id = GSM3200803 !Series_sample_id = GSM3200804 !Series_sample_id = GSM3200805 !Series_sample_id = GSM3200806 !Series_sample_id = GSM3200807 !Series_sample_id = GSM3200808 !Series_sample_id = GSM3200809 !Series_sample_id = GSM3200810 !Series_sample_id = GSM3200811 !Series_sample_id = GSM3200812 !Series_sample_id = GSM3200813 !Series_sample_id = GSM3200814 !Series_sample_id = GSM3200815 !Series_sample_id = GSM3200816 !Series_sample_id = GSM3200817 !Series_sample_id = GSM3200818 !Series_sample_id = GSM3200819 !Series_sample_id = GSM3200820 !Series_sample_id = GSM3200821 !Series_sample_id = GSM3200822 !Series_sample_id = GSM3200823 !Series_sample_id = GSM3200824 !Series_sample_id = GSM3200825 !Series_sample_id = GSM3200826 !Series_sample_id = GSM3200827 !Series_sample_id = GSM3200828 !Series_sample_id = GSM3200829 !Series_sample_id = GSM3200830 !Series_sample_id = GSM3200831 !Series_sample_id = GSM3200832 !Series_sample_id = GSM3200833 !Series_sample_id = GSM3200834 !Series_sample_id = GSM3200835 !Series_sample_id = GSM3200836 !Series_sample_id = GSM3200837 !Series_sample_id = GSM3200838 !Series_sample_id = GSM3200839 !Series_sample_id = GSM3200840 !Series_sample_id = GSM3200841 !Series_sample_id = GSM3200842 !Series_sample_id = GSM3200843 !Series_sample_id = GSM3200844 !Series_sample_id = GSM3200845 !Series_sample_id = GSM3200846 !Series_sample_id = GSM3200847 !Series_sample_id = GSM3200848 !Series_sample_id = GSM3200849 !Series_sample_id = GSM3200850 !Series_sample_id = GSM3200851 !Series_sample_id = GSM3200852 !Series_sample_id = GSM3200853 !Series_sample_id = GSM3200854 !Series_sample_id = GSM3200855 !Series_sample_id = GSM3200856 !Series_sample_id = GSM3200857 !Series_sample_id = GSM3200858 !Series_sample_id = GSM3200859 !Series_sample_id = GSM3200860 !Series_sample_id = GSM3200861 !Series_sample_id = GSM3200862 !Series_sample_id = GSM3200863 !Series_sample_id = GSM3200864 !Series_sample_id = GSM3200865 !Series_sample_id = GSM3200866 !Series_sample_id = GSM3200867 !Series_sample_id = GSM3200868 !Series_sample_id = GSM3200869 !Series_sample_id = GSM3200870 !Series_sample_id = GSM3200871 !Series_sample_id = GSM3200872 !Series_sample_id = GSM3200873 !Series_sample_id = GSM3200874 !Series_sample_id = GSM3200875 !Series_sample_id = GSM3200876 !Series_sample_id = GSM3200877 !Series_sample_id = GSM3200878 !Series_sample_id = GSM3200879 !Series_sample_id = GSM3200880 !Series_sample_id = GSM3200881 !Series_sample_id = GSM3200882 !Series_sample_id = GSM3200883 !Series_sample_id = GSM3200884 !Series_sample_id = GSM3200885 !Series_sample_id = GSM3200886 !Series_sample_id = GSM3200887 !Series_sample_id = GSM3200888 !Series_sample_id = GSM3200889 !Series_sample_id = GSM3200890 !Series_sample_id = GSM3200891 !Series_sample_id = GSM3200892 !Series_sample_id = GSM3200893 !Series_sample_id = GSM3200894 !Series_sample_id = GSM3200895 !Series_sample_id = GSM3200896 !Series_sample_id = GSM3200897 !Series_sample_id = GSM3200898 !Series_sample_id = GSM3200899 !Series_sample_id = GSM3200900 !Series_sample_id = GSM3200901 !Series_sample_id = GSM3200902 !Series_sample_id = GSM3200903 !Series_sample_id = GSM3200904 !Series_sample_id = GSM3200905 !Series_sample_id = GSM3200906 !Series_sample_id = GSM3200907 !Series_sample_id = GSM3200908 !Series_sample_id = GSM3200909 !Series_sample_id = GSM3200910 !Series_sample_id = GSM3200911 !Series_sample_id = GSM3200912 !Series_sample_id = GSM3200913 !Series_sample_id = GSM3200914 !Series_sample_id = GSM3200915 !Series_sample_id = GSM3200916 !Series_sample_id = GSM3200917 !Series_sample_id = GSM3200918 !Series_sample_id = GSM3200919 !Series_sample_id = GSM3200920 !Series_sample_id = GSM3200921 !Series_sample_id = GSM3200922 !Series_sample_id = GSM3200923 !Series_sample_id = GSM3200924 !Series_sample_id = GSM3200925 !Series_sample_id = GSM3200926 !Series_sample_id = GSM3200927 !Series_sample_id = GSM3200928 !Series_sample_id = GSM3200929 !Series_sample_id = GSM3200930 !Series_sample_id = GSM3200931 !Series_sample_id = GSM3200932 !Series_sample_id = GSM3200933 !Series_sample_id = GSM3200934 !Series_sample_id = GSM3200935 !Series_sample_id = GSM3200936 !Series_sample_id = GSM3200937 !Series_sample_id = GSM3200938 !Series_sample_id = GSM3200939 !Series_sample_id = GSM3200940 !Series_sample_id = GSM3200941 !Series_sample_id = GSM3200942 !Series_sample_id = GSM3200943 !Series_sample_id = GSM3200944 !Series_sample_id = GSM3200945 !Series_sample_id = GSM3200946 !Series_sample_id = GSM3200947 !Series_sample_id = GSM3200948 !Series_sample_id = GSM3200949 !Series_sample_id = GSM3200950 !Series_sample_id = GSM3200951 !Series_sample_id = GSM3200952 !Series_sample_id = GSM3200953 !Series_sample_id = GSM3200954 !Series_sample_id = GSM3200955 !Series_sample_id = GSM3200956 !Series_sample_id = GSM3200957 !Series_sample_id = GSM3200958 !Series_sample_id = GSM3200959 !Series_sample_id = GSM3200960 !Series_sample_id = GSM3200961 !Series_sample_id = GSM3200962 !Series_sample_id = GSM3200963 !Series_sample_id = GSM3200964 !Series_sample_id = GSM3200965 !Series_sample_id = GSM3200966 !Series_sample_id = GSM3200967 !Series_sample_id = GSM3200968 !Series_sample_id = GSM3200969 !Series_sample_id = GSM3200970 !Series_sample_id = GSM3200971 !Series_sample_id = GSM3200972 !Series_sample_id = GSM3200973 !Series_sample_id = GSM3200974 !Series_sample_id = GSM3200975 !Series_sample_id = GSM3200976 !Series_sample_id = GSM3200977 !Series_sample_id = GSM3200978 !Series_sample_id = GSM3200979 !Series_sample_id = GSM3200980 !Series_sample_id = GSM3200981 !Series_sample_id = GSM3200982 !Series_sample_id = GSM3200983 !Series_sample_id = GSM3200984 !Series_sample_id = GSM3200985 !Series_sample_id = GSM3200986 !Series_sample_id = GSM3200987 !Series_sample_id = GSM3200988 !Series_sample_id = GSM3200989 !Series_sample_id = GSM3200990 !Series_sample_id = GSM3200991 !Series_sample_id = GSM3200992 !Series_sample_id = GSM3200993 !Series_sample_id = GSM3200994 !Series_sample_id = GSM3200995 !Series_sample_id = GSM3200996 !Series_sample_id = GSM3200997 !Series_sample_id = GSM3200998 !Series_sample_id = GSM3200999 !Series_sample_id = GSM3201000 !Series_sample_id = GSM3201001 !Series_sample_id = GSM3201002 !Series_sample_id = GSM3201003 !Series_sample_id = GSM3201004 !Series_sample_id = GSM3201005 !Series_sample_id = GSM3201006 !Series_sample_id = GSM3201007 !Series_sample_id = GSM3201008 !Series_sample_id = GSM3201009 !Series_sample_id = GSM3201010 !Series_sample_id = GSM3201011 !Series_sample_id = GSM3201012 !Series_sample_id = GSM3201013 !Series_sample_id = GSM3201014 !Series_sample_id = GSM3201015 !Series_sample_id = GSM3201016 !Series_sample_id = GSM3201017 !Series_sample_id = GSM3201018 !Series_sample_id = GSM3201019 !Series_sample_id = GSM3201020 !Series_sample_id = GSM3201021 !Series_sample_id = GSM3201022 !Series_sample_id = GSM3201023 !Series_sample_id = GSM3201024 !Series_sample_id = GSM3201025 !Series_sample_id = GSM3201026 !Series_sample_id = GSM3201027 !Series_sample_id = GSM3201028 !Series_sample_id = GSM3201029 !Series_sample_id = GSM3201030 !Series_sample_id = GSM3201031 !Series_sample_id = GSM3201032 !Series_sample_id = GSM3201033 !Series_sample_id = GSM3201034 !Series_sample_id = GSM3201035 !Series_sample_id = GSM3201036 !Series_sample_id = GSM3201037 !Series_sample_id = GSM3201038 !Series_sample_id = GSM3201039 !Series_sample_id = GSM3201040 !Series_sample_id = GSM3201041 !Series_sample_id = GSM3201042 !Series_sample_id = GSM3201043 !Series_sample_id = GSM3201044 !Series_sample_id = GSM3201045 !Series_sample_id = GSM3201046 !Series_sample_id = GSM3201047 !Series_sample_id = GSM3201048 !Series_sample_id = GSM3201049 !Series_sample_id = GSM3201050 !Series_sample_id = GSM3201051 !Series_sample_id = GSM3201052 !Series_sample_id = GSM3201053 !Series_sample_id = GSM3201054 !Series_sample_id = GSM3201055 !Series_sample_id = GSM3201056 !Series_sample_id = GSM3201057 !Series_sample_id = GSM3201058 !Series_sample_id = GSM3201059 !Series_sample_id = GSM3201060 !Series_sample_id = GSM3201061 !Series_sample_id = GSM3201062 !Series_sample_id = GSM3201063 !Series_sample_id = GSM3201064 !Series_sample_id = GSM3201065 !Series_sample_id = GSM3201066 !Series_sample_id = GSM3201067 !Series_sample_id = GSM3201068 !Series_sample_id = GSM3201069 !Series_sample_id = GSM3201070 !Series_sample_id = GSM3201071 !Series_sample_id = GSM3201072 !Series_sample_id = GSM3201073 !Series_sample_id = GSM3201074 !Series_sample_id = GSM3201075 !Series_sample_id = GSM3201076 !Series_sample_id = GSM3201077 !Series_sample_id = GSM3201078 !Series_sample_id = GSM3201079 !Series_sample_id = GSM3201080 !Series_sample_id = GSM3201081 !Series_sample_id = GSM3201082 !Series_sample_id = GSM3201083 !Series_sample_id = GSM3201084 !Series_sample_id = GSM3201085 !Series_sample_id = GSM3201086 !Series_sample_id = GSM3201087 !Series_sample_id = GSM3201088 !Series_sample_id = GSM3201089 !Series_sample_id = GSM3201090 !Series_sample_id = GSM3201091 !Series_sample_id = GSM3201092 !Series_sample_id = GSM3201093 !Series_sample_id = GSM3201094 !Series_sample_id = GSM3201095 !Series_sample_id = GSM3201096 !Series_sample_id = GSM3201097 !Series_sample_id = GSM3201098 !Series_sample_id = GSM3201099 !Series_sample_id = GSM3201100 !Series_sample_id = GSM3201101 !Series_sample_id = GSM3201102 !Series_sample_id = GSM3201103 !Series_sample_id = GSM3201104 !Series_sample_id = GSM3201105 !Series_sample_id = GSM3201106 !Series_sample_id = GSM3201107 !Series_sample_id = GSM3201108 !Series_sample_id = GSM3201109 !Series_sample_id = GSM3201110 !Series_sample_id = GSM3201111 !Series_sample_id = GSM3201112 !Series_sample_id = GSM3201113 !Series_sample_id = GSM3201114 !Series_sample_id = GSM3201115 !Series_sample_id = GSM3201116 !Series_sample_id = GSM3201117 !Series_sample_id = GSM3201118 !Series_sample_id = GSM3201119 !Series_sample_id = GSM3201120 !Series_sample_id = GSM3201121 !Series_sample_id = GSM3201122 !Series_sample_id = GSM3201123 !Series_sample_id = GSM3201124 !Series_sample_id = GSM3201125 !Series_sample_id = GSM3201126 !Series_sample_id = GSM3201127 !Series_sample_id = GSM3201128 !Series_sample_id = GSM3201129 !Series_sample_id = GSM3201130 !Series_sample_id = GSM3201131 !Series_sample_id = GSM3201132 !Series_sample_id = GSM3201133 !Series_sample_id = GSM3201134 !Series_sample_id = GSM3201135 !Series_sample_id = GSM3201136 !Series_sample_id = GSM3201137 !Series_sample_id = GSM3201138 !Series_sample_id = GSM3201139 !Series_sample_id = GSM3201140 !Series_sample_id = GSM3201141 !Series_sample_id = GSM3201142 !Series_sample_id = GSM3201143 !Series_sample_id = GSM3201144 !Series_sample_id = GSM3201145 !Series_sample_id = GSM3201146 !Series_sample_id = GSM3201147 !Series_sample_id = GSM3201148 !Series_sample_id = GSM3201149 !Series_sample_id = GSM3201150 !Series_sample_id = GSM3201151 !Series_sample_id = GSM3201152 !Series_sample_id = GSM3201153 !Series_sample_id = GSM3201154 !Series_sample_id = GSM3201155 !Series_sample_id = GSM3201156 !Series_sample_id = GSM3201157 !Series_sample_id = GSM3201158 !Series_sample_id = GSM3201159 !Series_sample_id = GSM3201160 !Series_sample_id = GSM3201161 !Series_sample_id = GSM3201162 !Series_sample_id = GSM3201163 !Series_sample_id = GSM3201164 !Series_sample_id = GSM3201165 !Series_sample_id = GSM3201166 !Series_sample_id = GSM3201167 !Series_sample_id = GSM3201168 !Series_sample_id = GSM3201169 !Series_sample_id = GSM3201170 !Series_sample_id = GSM3201171 !Series_sample_id = GSM3201172 !Series_sample_id = GSM3201173 !Series_sample_id = GSM3201174 !Series_sample_id = GSM3201175 !Series_sample_id = GSM3201176 !Series_sample_id = GSM3201177 !Series_sample_id = GSM3201178 !Series_sample_id = GSM3201179 !Series_sample_id = GSM3201180 !Series_sample_id = GSM3201181 !Series_sample_id = GSM3201182 !Series_sample_id = GSM3201183 !Series_sample_id = GSM3201184 !Series_sample_id = GSM3201185 !Series_sample_id = GSM3201186 !Series_sample_id = GSM3201187 !Series_sample_id = GSM3201188 !Series_sample_id = GSM3201189 !Series_sample_id = GSM3201190 !Series_sample_id = GSM3201191 !Series_sample_id = GSM3201192 !Series_sample_id = GSM3201193 !Series_sample_id = GSM3201194 !Series_sample_id = GSM3201195 !Series_sample_id = GSM3201196 !Series_sample_id = GSM3201197 !Series_sample_id = GSM3201198 !Series_sample_id = GSM3201199 !Series_sample_id = GSM3201200 !Series_sample_id = GSM3201201 !Series_sample_id = GSM3201202 !Series_sample_id = GSM3201203 !Series_sample_id = GSM3201204 !Series_sample_id = GSM3201205 !Series_sample_id = GSM3201206 !Series_sample_id = GSM3201207 !Series_sample_id = GSM3201208 !Series_sample_id = GSM3201209 !Series_sample_id = GSM3201210 !Series_sample_id = GSM3201211 !Series_sample_id = GSM3201212 !Series_sample_id = GSM3201213 !Series_sample_id = GSM3201214 !Series_sample_id = GSM3201215 !Series_sample_id = GSM3201216 !Series_sample_id = GSM3201217 !Series_sample_id = GSM3201218 !Series_sample_id = GSM3201219 !Series_sample_id = GSM3201220 !Series_sample_id = GSM3201221 !Series_sample_id = GSM3201222 !Series_sample_id = GSM3201223 !Series_sample_id = GSM3201224 !Series_sample_id = GSM3201225 !Series_sample_id = GSM3201226 !Series_sample_id = GSM3201227 !Series_sample_id = GSM3201228 !Series_sample_id = GSM3201229 !Series_sample_id = GSM3201230 !Series_sample_id = GSM3201231 !Series_sample_id = GSM3201232 !Series_sample_id = GSM3201233 !Series_sample_id = GSM3201234 !Series_sample_id = GSM3201235 !Series_sample_id = GSM3201236 !Series_sample_id = GSM3201237 !Series_sample_id = GSM3201238 !Series_sample_id = GSM3201239 !Series_sample_id = GSM3201240 !Series_sample_id = GSM3201241 !Series_sample_id = GSM3201242 !Series_sample_id = GSM3201243 !Series_sample_id = GSM3201244 !Series_sample_id = GSM3201245 !Series_sample_id = GSM3201246 !Series_sample_id = GSM3201247 !Series_sample_id = GSM3201248 !Series_sample_id = GSM3201249 !Series_sample_id = GSM3201250 !Series_sample_id = GSM3201251 !Series_sample_id = GSM3201252 !Series_sample_id = GSM3201253 !Series_sample_id = GSM3201254 !Series_sample_id = GSM3201255 !Series_sample_id = GSM3201256 !Series_sample_id = GSM3201257 !Series_sample_id = GSM3201258 !Series_sample_id = GSM3201259 !Series_sample_id = GSM3201260 !Series_sample_id = GSM3201261 !Series_sample_id = GSM3201262 !Series_sample_id = GSM3201263 !Series_sample_id = GSM3201264 !Series_sample_id = GSM3201265 !Series_sample_id = GSM3201266 !Series_sample_id = GSM3201267 !Series_sample_id = GSM3201268 !Series_sample_id = GSM3201269 !Series_sample_id = GSM3201270 !Series_sample_id = GSM3201271 !Series_sample_id = GSM3201272 !Series_sample_id = GSM3201273 !Series_sample_id = GSM3201274 !Series_sample_id = GSM3201275 !Series_sample_id = GSM3201276 !Series_sample_id = GSM3201277 !Series_sample_id = GSM3201278 !Series_sample_id = GSM3201279 !Series_sample_id = GSM3201280 !Series_sample_id = GSM3201281 !Series_sample_id = GSM3201282 !Series_sample_id = GSM3201283 !Series_sample_id = GSM3201284 !Series_sample_id = GSM3201285 !Series_sample_id = GSM3201286 !Series_sample_id = GSM3201287 !Series_sample_id = GSM3201288 !Series_sample_id = GSM3201289 !Series_sample_id = GSM3201290 !Series_sample_id = GSM3201291 !Series_sample_id = GSM3201292 !Series_sample_id = GSM3201293 !Series_sample_id = GSM3201294 !Series_sample_id = GSM3201295 !Series_sample_id = GSM3201296 !Series_sample_id = GSM3201297 !Series_sample_id = GSM3201298 !Series_sample_id = GSM3201299 !Series_sample_id = GSM3201300 !Series_sample_id = GSM3201301 !Series_sample_id = GSM3201302 !Series_sample_id = GSM3201303 !Series_sample_id = GSM3201304 !Series_sample_id = GSM3201305 !Series_sample_id = GSM3201306 !Series_sample_id = GSM3201307 !Series_sample_id = GSM3201308 !Series_sample_id = GSM3201309 !Series_sample_id = GSM3201310 !Series_sample_id = GSM3201311 !Series_sample_id = GSM3201312 !Series_sample_id = GSM3201313 !Series_sample_id = GSM3201314 !Series_sample_id = GSM3201315 !Series_sample_id = GSM3201316 !Series_sample_id = GSM3201317 !Series_sample_id = GSM3201318 !Series_sample_id = GSM3201319 !Series_sample_id = GSM3201320 !Series_sample_id = GSM3201321 !Series_sample_id = GSM3201322 !Series_sample_id = GSM3201323 !Series_sample_id = GSM3201324 !Series_sample_id = GSM3201325 !Series_sample_id = GSM3201326 !Series_sample_id = GSM3201327 !Series_sample_id = GSM3201328 !Series_sample_id = GSM3201329 !Series_sample_id = GSM3201330 !Series_sample_id = GSM3201331 !Series_sample_id = GSM3201332 !Series_sample_id = GSM3201333 !Series_sample_id = GSM3201334 !Series_sample_id = GSM3201335 !Series_sample_id = GSM3201336 !Series_sample_id = GSM3201337 !Series_sample_id = GSM3201338 !Series_sample_id = GSM3201339 !Series_sample_id = GSM3201340 !Series_sample_id = GSM3201341 !Series_sample_id = GSM3201342 !Series_sample_id = GSM3201343 !Series_sample_id = GSM3201344 !Series_sample_id = GSM3201345 !Series_sample_id = GSM3201346 !Series_sample_id = GSM3201347 !Series_sample_id = GSM3201348 !Series_sample_id = GSM3201349 !Series_sample_id = GSM3201350 !Series_sample_id = GSM3201351 !Series_sample_id = GSM3201352 !Series_sample_id = GSM3201353 !Series_sample_id = GSM3201354 !Series_sample_id = GSM3201355 !Series_sample_id = GSM3201356 !Series_sample_id = GSM3201357 !Series_sample_id = GSM3201358 !Series_sample_id = GSM3201359 !Series_sample_id = GSM3201360 !Series_sample_id = GSM3201361 !Series_sample_id = GSM3201362 !Series_sample_id = GSM3201363 !Series_sample_id = GSM3201364 !Series_sample_id = GSM3201365 !Series_sample_id = GSM3201366 !Series_sample_id = GSM3201367 !Series_sample_id = GSM3201368 !Series_sample_id = GSM3201369 !Series_sample_id = GSM3201370 !Series_sample_id = GSM3201371 !Series_sample_id = GSM3201372 !Series_sample_id = GSM3201373 !Series_sample_id = GSM3201374 !Series_sample_id = GSM3201375 !Series_sample_id = GSM3201376 !Series_sample_id = GSM3201377 !Series_sample_id = GSM3201378 !Series_sample_id = GSM3201379 !Series_sample_id = GSM3201380 !Series_sample_id = GSM3201381 !Series_sample_id = GSM3201382 !Series_sample_id = GSM3201383 !Series_sample_id = GSM3201384 !Series_sample_id = GSM3201385 !Series_sample_id = GSM3201386 !Series_sample_id = GSM3201387 !Series_sample_id = GSM3201388 !Series_sample_id = GSM3201389 !Series_sample_id = GSM3201390 !Series_sample_id = GSM3201391 !Series_sample_id = GSM3201392 !Series_sample_id = GSM3201393 !Series_sample_id = GSM3201394 !Series_sample_id = GSM3201395 !Series_sample_id = GSM3201396 !Series_sample_id = GSM3201397 !Series_sample_id = GSM3201398 !Series_sample_id = GSM3201399 !Series_sample_id = GSM3201400 !Series_sample_id = GSM3201401 !Series_sample_id = GSM3201402 !Series_sample_id = GSM3201403 !Series_sample_id = GSM3201404 !Series_sample_id = GSM3201405 !Series_sample_id = GSM3201406 !Series_sample_id = GSM3201407 !Series_sample_id = GSM3201408 !Series_sample_id = GSM3201409 !Series_sample_id = GSM3201410 !Series_sample_id = GSM3201411 !Series_sample_id = GSM3201412 !Series_sample_id = GSM3201413 !Series_sample_id = GSM3201414 !Series_sample_id = GSM3201415 !Series_sample_id = GSM3201416 !Series_sample_id = GSM3201417 !Series_sample_id = GSM3201418 !Series_sample_id = GSM3201419 !Series_sample_id = GSM3201420 !Series_sample_id = GSM3201421 !Series_sample_id = GSM3201422 !Series_sample_id = GSM3201423 !Series_sample_id = GSM3201424 !Series_sample_id = GSM3201425 !Series_sample_id = GSM3201426 !Series_sample_id = GSM3201427 !Series_sample_id = GSM3201428 !Series_sample_id = GSM3201429 !Series_sample_id = GSM3201430 !Series_sample_id = GSM3201431 !Series_sample_id = GSM3201432 !Series_sample_id = GSM3201433 !Series_sample_id = GSM3201434 !Series_sample_id = GSM3201435 !Series_sample_id = GSM3201436 !Series_sample_id = GSM3201437 !Series_sample_id = GSM3201438 !Series_sample_id = GSM3201439 !Series_sample_id = GSM3201440 !Series_sample_id = GSM3201441 !Series_sample_id = GSM3201442 !Series_sample_id = GSM3201443 !Series_sample_id = GSM3201444 !Series_sample_id = GSM3201445 !Series_sample_id = GSM3201446 !Series_sample_id = GSM3201447 !Series_sample_id = GSM3201448 !Series_sample_id = GSM3201449 !Series_sample_id = GSM3201450 !Series_sample_id = GSM3201451 !Series_sample_id = GSM3201452 !Series_sample_id = GSM3201453 !Series_sample_id = GSM3201454 !Series_sample_id = GSM3201455 !Series_sample_id = GSM3201456 !Series_sample_id = GSM3201457 !Series_sample_id = GSM3201458 !Series_sample_id = GSM3201459 !Series_sample_id = GSM3201460 !Series_sample_id = GSM3201461 !Series_sample_id = GSM3201462 !Series_sample_id = GSM3201463 !Series_sample_id = GSM3201464 !Series_sample_id = GSM3201465 !Series_sample_id = GSM3201466 !Series_sample_id = GSM3201467 !Series_sample_id = GSM3201468 !Series_sample_id = GSM3201469 !Series_sample_id = GSM3201470 !Series_sample_id = GSM3201471 !Series_sample_id = GSM3201472 !Series_sample_id = GSM3201473 !Series_sample_id = GSM3201474 !Series_sample_id = GSM3201475 !Series_sample_id = GSM3201476 !Series_sample_id = GSM3201477 !Series_sample_id = GSM3201478 !Series_sample_id = GSM3201479 !Series_sample_id = GSM3201480 !Series_sample_id = GSM3201481 !Series_sample_id = GSM3201482 !Series_sample_id = GSM3201483 !Series_sample_id = GSM3201484 !Series_sample_id = GSM3201485 !Series_sample_id = GSM3201486 !Series_sample_id = GSM3201487 !Series_sample_id = GSM3201488 !Series_sample_id = GSM3201489 !Series_sample_id = GSM3201490 !Series_sample_id = GSM3201491 !Series_sample_id = GSM3201492 !Series_sample_id = GSM3201493 !Series_sample_id = GSM3201494 !Series_sample_id = GSM3201495 !Series_sample_id = GSM3201496 !Series_sample_id = GSM3201497 !Series_sample_id = GSM3201498 !Series_sample_id = GSM3201499 !Series_sample_id = GSM3201500 !Series_sample_id = GSM3201501 !Series_sample_id = GSM3201502 !Series_sample_id = GSM3201503 !Series_sample_id = GSM3201504 !Series_sample_id = GSM3201505 !Series_sample_id = GSM3201506 !Series_sample_id = GSM3201507 !Series_sample_id = GSM3201508 !Series_sample_id = GSM3201509 !Series_sample_id = GSM3201510 !Series_sample_id = GSM3201511 !Series_sample_id = GSM3201512 !Series_sample_id = GSM3201513 !Series_sample_id = GSM3201514 !Series_sample_id = GSM3201515 !Series_sample_id = GSM3201516 !Series_sample_id = GSM3201517 !Series_sample_id = GSM3201518 !Series_sample_id = GSM3201519 !Series_sample_id = GSM3201520 !Series_sample_id = GSM3201521 !Series_sample_id = GSM3201522 !Series_sample_id = GSM3201523 !Series_sample_id = GSM3201524 !Series_sample_id = GSM3201525 !Series_sample_id = GSM3201526 !Series_sample_id = GSM3201527 !Series_sample_id = GSM3201528 !Series_sample_id = GSM3201529 !Series_sample_id = GSM3201530 !Series_sample_id = GSM3201531 !Series_sample_id = GSM3201532 !Series_sample_id = GSM3201533 !Series_sample_id = GSM3201534 !Series_sample_id = GSM3201535 !Series_sample_id = GSM3201536 !Series_sample_id = GSM3201537 !Series_sample_id = GSM3201538 !Series_sample_id = GSM3201539 !Series_sample_id = GSM3201540 !Series_sample_id = GSM3201541 !Series_sample_id = GSM3201542 !Series_sample_id = GSM3201543 !Series_sample_id = GSM3201544 !Series_sample_id = GSM3201545 !Series_sample_id = GSM3201546 !Series_sample_id = GSM3201547 !Series_sample_id = GSM3201548 !Series_sample_id = GSM3201549 !Series_sample_id = GSM3201550 !Series_sample_id = GSM3201551 !Series_sample_id = GSM3201552 !Series_sample_id = GSM3201553 !Series_sample_id = GSM3201554 !Series_sample_id = GSM3201555 !Series_sample_id = GSM3201556 !Series_sample_id = GSM3201557 !Series_sample_id = GSM3201558 !Series_sample_id = GSM3201559 !Series_sample_id = GSM3201560 !Series_sample_id = GSM3201561 !Series_sample_id = GSM3201562 !Series_sample_id = GSM3201563 !Series_sample_id = GSM3201564 !Series_sample_id = GSM3201565 !Series_sample_id = GSM3201566 !Series_sample_id = GSM3201567 !Series_sample_id = GSM3201568 !Series_sample_id = GSM3201569 !Series_sample_id = GSM3201570 !Series_sample_id = GSM3201571 !Series_sample_id = GSM3201572 !Series_sample_id = GSM3201573 !Series_sample_id = GSM3201574 !Series_sample_id = GSM3201575 !Series_sample_id = GSM3201576 !Series_sample_id = GSM3201577 !Series_sample_id = GSM3201578 !Series_sample_id = GSM3201579 !Series_sample_id = GSM3201580 !Series_sample_id = GSM3201581 !Series_sample_id = GSM3201582 !Series_sample_id = GSM3201583 !Series_sample_id = GSM3201584 !Series_sample_id = GSM3201585 !Series_sample_id = GSM3201586 !Series_sample_id = GSM3201587 !Series_sample_id = GSM3201588 !Series_sample_id = GSM3201589 !Series_sample_id = GSM3201590 !Series_sample_id = GSM3201591 !Series_sample_id = GSM3201592 !Series_sample_id = GSM3201593 !Series_sample_id = GSM3201594 !Series_sample_id = GSM3201595 !Series_sample_id = GSM3201596 !Series_sample_id = GSM3201597 !Series_sample_id = GSM3201598 !Series_sample_id = GSM3201599 !Series_sample_id = GSM3201600 !Series_sample_id = GSM3201601 !Series_sample_id = GSM3201602 !Series_sample_id = GSM3201603 !Series_sample_id = GSM3201604 !Series_sample_id = GSM3201605 !Series_sample_id = GSM3201606 !Series_sample_id = GSM3201607 !Series_sample_id = GSM3201608 !Series_sample_id = GSM3201609 !Series_sample_id = GSM3201610 !Series_sample_id = GSM3201611 !Series_sample_id = GSM3201612 !Series_sample_id = GSM3201613 !Series_sample_id = GSM3201614 !Series_sample_id = GSM3201615 !Series_sample_id = GSM3201616 !Series_sample_id = GSM3201617 !Series_sample_id = GSM3201618 !Series_sample_id = GSM3201619 !Series_sample_id = GSM3201620 !Series_sample_id = GSM3201621 !Series_sample_id = GSM3201622 !Series_sample_id = GSM3201623 !Series_sample_id = GSM3201624 !Series_sample_id = GSM3201625 !Series_sample_id = GSM3201626 !Series_sample_id = GSM3201627 !Series_sample_id = GSM3201628 !Series_sample_id = GSM3201629 !Series_sample_id = GSM3201630 !Series_sample_id = GSM3201631 !Series_sample_id = GSM3201632 !Series_sample_id = GSM3201633 !Series_sample_id = GSM3201634 !Series_sample_id = GSM3201635 !Series_sample_id = GSM3201636 !Series_sample_id = GSM3201637 !Series_sample_id = GSM3201638 !Series_sample_id = GSM3201639 !Series_sample_id = GSM3201640 !Series_sample_id = GSM3201641 !Series_sample_id = GSM3201642 !Series_sample_id = GSM3201643 !Series_sample_id = GSM3201644 !Series_sample_id = GSM3201645 !Series_sample_id = GSM3201646 !Series_sample_id = GSM3201647 !Series_sample_id = GSM3201648 !Series_sample_id = GSM3201649 !Series_sample_id = GSM3201650 !Series_sample_id = GSM3201651 !Series_sample_id = GSM3201652 !Series_sample_id = GSM3201653 !Series_sample_id = GSM3201654 !Series_sample_id = GSM3201655 !Series_sample_id = GSM3201656 !Series_sample_id = GSM3201657 !Series_sample_id = GSM3201658 !Series_sample_id = GSM3201659 !Series_sample_id = GSM3201660 !Series_sample_id = GSM3201661 !Series_sample_id = GSM3201662 !Series_sample_id = GSM3201663 !Series_sample_id = GSM3201664 !Series_sample_id = GSM3201665 !Series_sample_id = GSM3201666 !Series_sample_id = GSM3201667 !Series_sample_id = GSM3201668 !Series_sample_id = GSM3201669 !Series_sample_id = GSM3201670 !Series_sample_id = GSM3201671 !Series_sample_id = GSM3201672 !Series_sample_id = GSM3201673 !Series_sample_id = GSM3201674 !Series_sample_id = GSM3201675 !Series_sample_id = GSM3201676 !Series_sample_id = GSM3201677 !Series_sample_id = GSM3201678 !Series_sample_id = GSM3201679 !Series_sample_id = GSM3201680 !Series_sample_id = GSM3201681 !Series_sample_id = GSM3201682 !Series_sample_id = GSM3201683 !Series_sample_id = GSM3201684 !Series_sample_id = GSM3201685 !Series_sample_id = GSM3201686 !Series_sample_id = GSM3201687 !Series_sample_id = GSM3201688 !Series_sample_id = GSM3201689 !Series_sample_id = GSM3201690 !Series_sample_id = GSM3201691 !Series_sample_id = GSM3201692 !Series_sample_id = GSM3201693 !Series_sample_id = GSM3201694 !Series_sample_id = GSM3201695 !Series_sample_id = GSM3201696 !Series_sample_id = GSM3201697 !Series_sample_id = GSM3201698 !Series_sample_id = GSM3201699 !Series_sample_id = GSM3201700 !Series_sample_id = GSM3201701 !Series_sample_id = GSM3201702 !Series_sample_id = GSM3201703 !Series_sample_id = GSM3201704 !Series_sample_id = GSM3201705 !Series_sample_id = GSM3201706 !Series_sample_id = GSM3201707 !Series_sample_id = GSM3201708 !Series_sample_id = GSM3201709 !Series_sample_id = GSM3201710 !Series_sample_id = GSM3201711 !Series_sample_id = GSM3201712 !Series_sample_id = GSM3201713 !Series_sample_id = GSM3201714 !Series_sample_id = GSM3201715 !Series_sample_id = GSM3201716 !Series_sample_id = GSM3201717 !Series_sample_id = GSM3201718 !Series_sample_id = GSM3201719 !Series_sample_id = GSM3201720 !Series_sample_id = GSM3201721 !Series_sample_id = GSM3201722 !Series_sample_id = GSM3201723 !Series_sample_id = GSM3201724 !Series_sample_id = GSM3201725 !Series_sample_id = GSM3201726 !Series_sample_id = GSM3201727 !Series_sample_id = GSM3201728 !Series_sample_id = GSM3201729 !Series_sample_id = GSM3201730 !Series_sample_id = GSM3201731 !Series_sample_id = GSM3201732 !Series_sample_id = GSM3201733 !Series_sample_id = GSM3201734 !Series_sample_id = GSM3201735 !Series_sample_id = GSM3201736 !Series_sample_id = GSM3201737 !Series_sample_id = GSM3201738 !Series_sample_id = GSM3201739 !Series_sample_id = GSM3201740 !Series_sample_id = GSM3201741 !Series_sample_id = GSM3201742 !Series_sample_id = GSM3201743 !Series_sample_id = GSM3201744 !Series_sample_id = GSM3201745 !Series_sample_id = GSM3201746 !Series_sample_id = GSM3201747 !Series_sample_id = GSM3201748 !Series_sample_id = GSM3201749 !Series_sample_id = GSM3201750 !Series_sample_id = GSM3201751 !Series_sample_id = GSM3201752 !Series_sample_id = GSM3201753 !Series_sample_id = GSM3201754 !Series_sample_id = GSM3201755 !Series_sample_id = GSM3201756 !Series_sample_id = GSM3201757 !Series_sample_id = GSM3201758 !Series_sample_id = GSM3201759 !Series_sample_id = GSM3201760 !Series_sample_id = GSM3201761 !Series_sample_id = GSM3201762 !Series_sample_id = GSM3201763 !Series_sample_id = GSM3201764 !Series_sample_id = GSM3201765 !Series_sample_id = GSM3201766 !Series_sample_id = GSM3201767 !Series_sample_id = GSM3201768 !Series_sample_id = GSM3201769 !Series_sample_id = GSM3201770 !Series_sample_id = GSM3201771 !Series_sample_id = GSM3201772 !Series_sample_id = GSM3201773 !Series_sample_id = GSM3201774 !Series_sample_id = GSM3201775 !Series_sample_id = GSM3201776 !Series_sample_id = GSM3201777 !Series_sample_id = GSM3201778 !Series_sample_id = GSM3201779 !Series_sample_id = GSM3201780 !Series_sample_id = GSM3201781 !Series_sample_id = GSM3201782 !Series_sample_id = GSM3201783 !Series_sample_id = GSM3201784 !Series_sample_id = GSM3201785 !Series_sample_id = GSM3201786 !Series_sample_id = GSM3201787 !Series_sample_id = GSM3201788 !Series_sample_id = GSM3201789 !Series_sample_id = GSM3201790 !Series_sample_id = GSM3201791 !Series_sample_id = GSM3201792 !Series_sample_id = GSM3201793 !Series_sample_id = GSM3201794 !Series_sample_id = GSM3201795 !Series_sample_id = GSM3201796 !Series_sample_id = GSM3201797 !Series_sample_id = GSM3201798 !Series_sample_id = GSM3201799 !Series_sample_id = GSM3201800 !Series_sample_id = GSM3201801 !Series_sample_id = GSM3201802 !Series_sample_id = GSM3201803 !Series_sample_id = GSM3201804 !Series_sample_id = GSM3201805 !Series_sample_id = GSM3201806 !Series_sample_id = GSM3201807 !Series_sample_id = GSM3201808 !Series_sample_id = GSM3201809 !Series_sample_id = GSM3201810 !Series_sample_id = GSM3201811 !Series_sample_id = GSM3201812 !Series_sample_id = GSM3201813 !Series_sample_id = GSM3201814 !Series_sample_id = GSM3201815 !Series_sample_id = GSM3201816 !Series_sample_id = GSM3201817 !Series_sample_id = GSM3201818 !Series_sample_id = GSM3201819 !Series_sample_id = GSM3201820 !Series_sample_id = GSM3201821 !Series_sample_id = GSM3201822 !Series_sample_id = GSM3201823 !Series_sample_id = GSM3201824 !Series_sample_id = GSM3201825 !Series_sample_id = GSM3201826 !Series_sample_id = GSM3201827 !Series_sample_id = GSM3201828 !Series_sample_id = GSM3201829 !Series_sample_id = GSM3201830 !Series_sample_id = GSM3201831 !Series_sample_id = GSM3201832 !Series_sample_id = GSM3201833 !Series_sample_id = GSM3201834 !Series_sample_id = GSM3201835 !Series_sample_id = GSM3201836 !Series_sample_id = GSM3201837 !Series_sample_id = GSM3201838 !Series_sample_id = GSM3201839 !Series_sample_id = GSM3201840 !Series_sample_id = GSM3201841 !Series_sample_id = GSM3201842 !Series_sample_id = GSM3201843 !Series_sample_id = GSM3201844 !Series_sample_id = GSM3201845 !Series_sample_id = GSM3201846 !Series_sample_id = GSM3201847 !Series_sample_id = GSM3201848 !Series_sample_id = GSM3201849 !Series_sample_id = GSM3201850 !Series_sample_id = GSM3201851 !Series_sample_id = GSM3201852 !Series_sample_id = GSM3201853 !Series_sample_id = GSM3201854 !Series_sample_id = GSM3201855 !Series_sample_id = GSM3201856 !Series_sample_id = GSM3201857 !Series_sample_id = GSM3201858 !Series_sample_id = GSM3201859 !Series_sample_id = GSM3201860 !Series_sample_id = GSM3201861 !Series_sample_id = GSM3201862 !Series_sample_id = GSM3201863 !Series_sample_id = GSM3201864 !Series_sample_id = GSM3201865 !Series_sample_id = GSM3201866 !Series_sample_id = GSM3201867 !Series_sample_id = GSM3201868 !Series_sample_id = GSM3201869 !Series_sample_id = GSM3201870 !Series_sample_id = GSM3201871 !Series_sample_id = GSM3201872 !Series_sample_id = GSM3201873 !Series_sample_id = GSM3201874 !Series_sample_id = GSM3201875 !Series_sample_id = GSM3201876 !Series_sample_id = GSM3201877 !Series_sample_id = GSM3201878 !Series_sample_id = GSM3201879 !Series_sample_id = GSM3201880 !Series_sample_id = GSM3201881 !Series_sample_id = GSM3201882 !Series_sample_id = GSM3201883 !Series_sample_id = GSM3201884 !Series_sample_id = GSM3201885 !Series_sample_id = GSM3201886 !Series_sample_id = GSM3201887 !Series_sample_id = GSM3201888 !Series_sample_id = GSM3201889 !Series_sample_id = GSM3201890 !Series_sample_id = GSM3201891 !Series_sample_id = GSM3201892 !Series_sample_id = GSM3201893 !Series_sample_id = GSM3201894 !Series_sample_id = GSM3201895 !Series_sample_id = GSM3201896 !Series_sample_id = GSM3201897 !Series_sample_id = GSM3201898 !Series_sample_id = GSM3201899 !Series_sample_id = GSM3201900 !Series_sample_id = GSM3201901 !Series_sample_id = GSM3201902 !Series_sample_id = GSM3201903 !Series_sample_id = GSM3201904 !Series_sample_id = GSM3201905 !Series_sample_id = GSM3201906 !Series_sample_id = GSM3201907 !Series_sample_id = GSM3201908 !Series_sample_id = GSM3201909 !Series_sample_id = GSM3201910 !Series_sample_id = GSM3201911 !Series_sample_id = GSM3201912 !Series_sample_id = GSM3201913 !Series_sample_id = GSM3201914 !Series_sample_id = GSM3201915 !Series_sample_id = GSM3201916 !Series_sample_id = GSM3201917 !Series_sample_id = GSM3201918 !Series_sample_id = GSM3201919 !Series_sample_id = GSM3201920 !Series_sample_id = GSM3201921 !Series_sample_id = GSM3201922 !Series_sample_id = GSM3201923 !Series_sample_id = GSM3201924 !Series_sample_id = GSM3201925 !Series_sample_id = GSM3201926 !Series_sample_id = GSM3201927 !Series_sample_id = GSM3201928 !Series_sample_id = GSM3201929 !Series_sample_id = GSM3201930 !Series_sample_id = GSM3201931 !Series_sample_id = GSM3201932 !Series_sample_id = GSM3201933 !Series_sample_id = GSM3201934 !Series_sample_id = GSM3201935 !Series_sample_id = GSM3201936 !Series_sample_id = GSM3201937 !Series_sample_id = GSM3201938 !Series_sample_id = GSM3201939 !Series_sample_id = GSM3201940 !Series_sample_id = GSM3201941 !Series_sample_id = GSM3201942 !Series_sample_id = GSM3201943 !Series_sample_id = GSM3201944 !Series_sample_id = GSM3201945 !Series_sample_id = GSM3201946 !Series_sample_id = GSM3201947 !Series_sample_id = GSM3201948 !Series_sample_id = GSM3201949 !Series_sample_id = GSM3201950 !Series_sample_id = GSM3201951 !Series_sample_id = GSM3201952 !Series_sample_id = GSM3201953 !Series_sample_id = GSM3201954 !Series_sample_id = GSM3201955 !Series_sample_id = GSM3201956 !Series_sample_id = GSM3201957 !Series_sample_id = GSM3201958 !Series_sample_id = GSM3201959 !Series_sample_id = GSM3201960 !Series_sample_id = GSM3201961 !Series_sample_id = GSM3201962 !Series_sample_id = GSM3201963 !Series_sample_id = GSM3201964 !Series_sample_id = GSM3201965 !Series_sample_id = GSM3201966 !Series_sample_id = GSM3201967 !Series_sample_id = GSM3201968 !Series_sample_id = GSM3201969 !Series_sample_id = GSM3201970 !Series_sample_id = GSM3201971 !Series_sample_id = GSM3201972 !Series_sample_id = GSM3201973 !Series_sample_id = GSM3201974 !Series_sample_id = GSM3201975 !Series_sample_id = GSM3201976 !Series_sample_id = GSM3201977 !Series_sample_id = GSM3201978 !Series_sample_id = GSM3201979 !Series_sample_id = GSM3201980 !Series_sample_id = GSM3201981 !Series_sample_id = GSM3201982 !Series_sample_id = GSM3201983 !Series_sample_id = GSM3201984 !Series_sample_id = GSM3201985 !Series_sample_id = GSM3201986 !Series_sample_id = GSM3201987 !Series_sample_id = GSM3201988 !Series_sample_id = GSM3201989 !Series_sample_id = GSM3201990 !Series_sample_id = GSM3201991 !Series_sample_id = GSM3201992 !Series_sample_id = GSM3201993 !Series_sample_id = GSM3201994 !Series_sample_id = GSM3201995 !Series_sample_id = GSM3201996 !Series_sample_id = GSM3201997 !Series_sample_id = GSM3201998 !Series_sample_id = GSM3201999 !Series_sample_id = GSM3202000 !Series_sample_id = GSM3202001 !Series_sample_id = GSM3202002 !Series_sample_id = GSM3202003 !Series_sample_id = GSM3202004 !Series_sample_id = GSM3202005 !Series_sample_id = GSM3202006 !Series_sample_id = GSM3202007 !Series_sample_id = GSM3202008 !Series_sample_id = GSM3202009 !Series_sample_id = GSM3202010 !Series_sample_id = GSM3202011 !Series_sample_id = GSM3202012 !Series_sample_id = GSM3202013 !Series_sample_id = GSM3202014 !Series_sample_id = GSM3202015 !Series_sample_id = GSM3202016 !Series_sample_id = GSM3202017 !Series_sample_id = GSM3202018 !Series_sample_id = GSM3202019 !Series_sample_id = GSM3202020 !Series_sample_id = GSM3202021 !Series_sample_id = GSM3202022 !Series_sample_id = GSM3202023 !Series_sample_id = GSM3202024 !Series_sample_id = GSM3202025 !Series_sample_id = GSM3202026 !Series_sample_id = GSM3202027 !Series_sample_id = GSM3202028 !Series_sample_id = GSM3202029 !Series_sample_id = GSM3202030 !Series_sample_id = GSM3202031 !Series_sample_id = GSM3202032 !Series_sample_id = GSM3202033 !Series_sample_id = GSM3202034 !Series_sample_id = GSM3202035 !Series_sample_id = GSM3202036 !Series_sample_id = GSM3202037 !Series_sample_id = GSM3202038 !Series_sample_id = GSM3202039 !Series_sample_id = GSM3202040 !Series_sample_id = GSM3202041 !Series_sample_id = GSM3202042 !Series_sample_id = GSM3202043 !Series_sample_id = GSM3202044 !Series_sample_id = GSM3202045 !Series_sample_id = GSM3202046 !Series_sample_id = GSM3202047 !Series_sample_id = GSM3202048 !Series_sample_id = GSM3202049 !Series_sample_id = GSM3202050 !Series_sample_id = GSM3202051 !Series_sample_id = GSM3202052 !Series_sample_id = GSM3202053 !Series_sample_id = GSM3202054 !Series_sample_id = GSM3202055 !Series_sample_id = GSM3202056 !Series_sample_id = GSM3202057 !Series_sample_id = GSM3202058 !Series_sample_id = GSM3202059 !Series_sample_id = GSM3202060 !Series_sample_id = GSM3202061 !Series_sample_id = GSM3202062 !Series_sample_id = GSM3202063 !Series_sample_id = GSM3202064 !Series_sample_id = GSM3202065 !Series_sample_id = GSM3202066 !Series_sample_id = GSM3202067 !Series_sample_id = GSM3202068 !Series_sample_id = GSM3202069 !Series_sample_id = GSM3202070 !Series_sample_id = GSM3202071 !Series_sample_id = GSM3202072 !Series_sample_id = GSM3202073 !Series_sample_id = GSM3202074 !Series_sample_id = GSM3202075 !Series_sample_id = GSM3202076 !Series_sample_id = GSM3202077 !Series_sample_id = GSM3202078 !Series_sample_id = GSM3202079 !Series_sample_id = GSM3202080 !Series_sample_id = GSM3202081 !Series_sample_id = GSM3202082 !Series_sample_id = GSM3202083 !Series_sample_id = GSM3202084 !Series_sample_id = GSM3202085 !Series_sample_id = GSM3202086 !Series_sample_id = GSM3202087 !Series_sample_id = GSM3202088 !Series_sample_id = GSM3202089 !Series_sample_id = GSM3202090 !Series_sample_id = GSM3202091 !Series_sample_id = GSM3202092 !Series_sample_id = GSM3202093 !Series_sample_id = GSM3202094 !Series_sample_id = GSM3202095 !Series_sample_id = GSM3202096 !Series_sample_id = GSM3202097 !Series_sample_id = GSM3202098 !Series_sample_id = GSM3202099 !Series_sample_id = GSM3202100 !Series_sample_id = GSM3202101 !Series_sample_id = GSM3202102 !Series_sample_id = GSM3202103 !Series_sample_id = GSM3202104 !Series_sample_id = GSM3202105 !Series_sample_id = GSM3202106 !Series_sample_id = GSM3202107 !Series_sample_id = GSM3202108 !Series_sample_id = GSM3202109 !Series_sample_id = GSM3202110 !Series_sample_id = GSM3202111 !Series_sample_id = GSM3202112 !Series_sample_id = GSM3202113 !Series_sample_id = GSM3202114 !Series_sample_id = GSM3202115 !Series_sample_id = GSM3202116 !Series_sample_id = GSM3202117 !Series_sample_id = GSM3202118 !Series_sample_id = GSM3202119 !Series_sample_id = GSM3202120 !Series_sample_id = GSM3202121 !Series_sample_id = GSM3202122 !Series_sample_id = GSM3202123 !Series_sample_id = GSM3202124 !Series_sample_id = GSM3202125 !Series_sample_id = GSM3202126 !Series_sample_id = GSM3202127 !Series_sample_id = GSM3202128 !Series_sample_id = GSM3202129 !Series_sample_id = GSM3202130 !Series_sample_id = GSM3202131 !Series_sample_id = GSM3202132 !Series_sample_id = GSM3202133 !Series_sample_id = GSM3202134 !Series_sample_id = GSM3202135 !Series_sample_id = GSM3202136 !Series_sample_id = GSM3202137 !Series_sample_id = GSM3202138 !Series_sample_id = GSM3202139 !Series_sample_id = GSM3202140 !Series_sample_id = GSM3202141 !Series_sample_id = GSM3202142 !Series_sample_id = GSM3202143 !Series_sample_id = GSM3202144 !Series_sample_id = GSM3202145 !Series_sample_id = GSM3202146 !Series_sample_id = GSM3202147 !Series_sample_id = GSM3202148 !Series_sample_id = GSM3202149 !Series_sample_id = GSM3202150 !Series_sample_id = GSM3202151 !Series_sample_id = GSM3202152 !Series_sample_id = GSM3202153 !Series_sample_id = GSM3202154 !Series_sample_id = GSM3202155 !Series_sample_id = GSM3202156 !Series_sample_id = GSM3202157 !Series_sample_id = GSM3202158 !Series_sample_id = GSM3202159 !Series_sample_id = GSM3202160 !Series_sample_id = GSM3202161 !Series_sample_id = GSM3202162 !Series_sample_id = GSM3202163 !Series_sample_id = GSM3202164 !Series_sample_id = GSM3202165 !Series_sample_id = GSM3202166 !Series_sample_id = GSM3202167 !Series_sample_id = GSM3202168 !Series_sample_id = GSM3202169 !Series_sample_id = GSM3202170 !Series_sample_id = GSM3202171 !Series_sample_id = GSM3202172 !Series_sample_id = GSM3202173 !Series_sample_id = GSM3202174 !Series_sample_id = GSM3202175 !Series_sample_id = GSM3202176 !Series_sample_id = GSM3202177 !Series_sample_id = GSM3202178 !Series_sample_id = GSM3202179 !Series_sample_id = GSM3202180 !Series_sample_id = GSM3202181 !Series_sample_id = GSM3202182 !Series_sample_id = GSM3202183 !Series_sample_id = GSM3202184 !Series_sample_id = GSM3202185 !Series_sample_id = GSM3202186 !Series_sample_id = GSM3202187 !Series_sample_id = GSM3202188 !Series_sample_id = GSM3202189 !Series_sample_id = GSM3202190 !Series_sample_id = GSM3202191 !Series_sample_id = GSM3202192 !Series_sample_id = GSM3202193 !Series_sample_id = GSM3202194 !Series_sample_id = GSM3202195 !Series_sample_id = GSM3202196 !Series_sample_id = GSM3202197 !Series_sample_id = GSM3202198 !Series_sample_id = GSM3202199 !Series_sample_id = GSM3202200 !Series_sample_id = GSM3202201 !Series_sample_id = GSM3202202 !Series_sample_id = GSM3202203 !Series_sample_id = GSM3202204 !Series_sample_id = GSM3202205 !Series_sample_id = GSM3202206 !Series_sample_id = GSM3202207 !Series_sample_id = GSM3202208 !Series_sample_id = GSM3202209 !Series_sample_id = GSM3202210 !Series_sample_id = GSM3202211 !Series_sample_id = GSM3202212 !Series_sample_id = GSM3202213 !Series_sample_id = GSM3202214 !Series_sample_id = GSM3202215 !Series_sample_id = GSM3202216 !Series_sample_id = GSM3202217 !Series_sample_id = GSM3202218 !Series_sample_id = GSM3202219 !Series_sample_id = GSM3202220 !Series_sample_id = GSM3202221 !Series_sample_id = GSM3202222 !Series_sample_id = GSM3202223 !Series_sample_id = GSM3202224 !Series_sample_id = GSM3202225 !Series_sample_id = GSM3202226 !Series_sample_id = GSM3202227 !Series_sample_id = GSM3202228 !Series_sample_id = GSM3202229 !Series_sample_id = GSM3202230 !Series_sample_id = GSM3202231 !Series_sample_id = GSM3202232 !Series_sample_id = GSM3202233 !Series_sample_id = GSM3202234 !Series_sample_id = GSM3202235 !Series_sample_id = GSM3202236 !Series_sample_id = GSM3202237 !Series_sample_id = GSM3202238 !Series_sample_id = GSM3202239 !Series_sample_id = GSM3202240 !Series_sample_id = GSM3202241 !Series_sample_id = GSM3202242 !Series_sample_id = GSM3202243 !Series_sample_id = GSM3202244 !Series_sample_id = GSM3202245 !Series_sample_id = GSM3202246 !Series_sample_id = GSM3202247 !Series_sample_id = GSM3202248 !Series_sample_id = GSM3202249 !Series_sample_id = GSM3202250 !Series_sample_id = GSM3202251 !Series_sample_id = GSM3202252 !Series_sample_id = GSM3202253 !Series_sample_id = GSM3202254 !Series_sample_id = GSM3202255 !Series_sample_id = GSM3202256 !Series_sample_id = GSM3202257 !Series_sample_id = GSM3202258 !Series_sample_id = GSM3202259 !Series_sample_id = GSM3202260 !Series_sample_id = GSM3202261 !Series_sample_id = GSM3202262 !Series_sample_id = GSM3202263 !Series_sample_id = GSM3202264 !Series_sample_id = GSM3202265 !Series_sample_id = GSM3202266 !Series_sample_id = GSM3202267 !Series_sample_id = GSM3202268 !Series_sample_id = GSM3202269 !Series_sample_id = GSM3202270 !Series_sample_id = GSM3202271 !Series_sample_id = GSM3202272 !Series_sample_id = GSM3202273 !Series_sample_id = GSM3202274 !Series_sample_id = GSM3202275 !Series_sample_id = GSM3202276 !Series_sample_id = GSM3202277 !Series_sample_id = GSM3202278 !Series_sample_id = GSM3202279 !Series_sample_id = GSM3202280 !Series_sample_id = GSM3202281 !Series_sample_id = GSM3202282 !Series_sample_id = GSM3202283 !Series_sample_id = GSM3202284 !Series_sample_id = GSM3202285 !Series_sample_id = GSM3202286 !Series_sample_id = GSM3202287 !Series_sample_id = GSM3202288 !Series_sample_id = GSM3202289 !Series_sample_id = GSM3202290 !Series_sample_id = GSM3202291 !Series_sample_id = GSM3202292 !Series_sample_id = GSM3202293 !Series_sample_id = GSM3202294 !Series_sample_id = GSM3202295 !Series_sample_id = GSM3202296 !Series_sample_id = GSM3202297 !Series_sample_id = GSM3202298 !Series_sample_id = GSM3202299 !Series_sample_id = GSM3202300 !Series_sample_id = GSM3202301 !Series_sample_id = GSM3202302 !Series_sample_id = GSM3202303 !Series_sample_id = GSM3202304 !Series_sample_id = GSM3202305 !Series_sample_id = GSM3202306 !Series_sample_id = GSM3202307 !Series_sample_id = GSM3202308 !Series_sample_id = GSM3202309 !Series_sample_id = GSM3202310 !Series_sample_id = GSM3202311 !Series_sample_id = GSM3202312 !Series_sample_id = GSM3202313 !Series_sample_id = GSM3202314 !Series_sample_id = GSM3202315 !Series_sample_id = GSM3202316 !Series_sample_id = GSM3202317 !Series_sample_id = GSM3202318 !Series_sample_id = GSM3202319 !Series_sample_id = GSM3202320 !Series_sample_id = GSM3202321 !Series_sample_id = GSM3202322 !Series_sample_id = GSM3202323 !Series_sample_id = GSM3202324 !Series_sample_id = GSM3202325 !Series_sample_id = GSM3202326 !Series_sample_id = GSM3202327 !Series_sample_id = GSM3202328 !Series_sample_id = GSM3202329 !Series_sample_id = GSM3202330 !Series_sample_id = GSM3202331 !Series_sample_id = GSM3202332 !Series_sample_id = GSM3202333 !Series_sample_id = GSM3202334 !Series_sample_id = GSM3202335 !Series_sample_id = GSM3202336 !Series_sample_id = GSM3202337 !Series_sample_id = GSM3202338 !Series_sample_id = GSM3202339 !Series_sample_id = GSM3202340 !Series_sample_id = GSM3202341 !Series_sample_id = GSM3202342 !Series_sample_id = GSM3202343 !Series_sample_id = GSM3202344 !Series_sample_id = GSM3202345 !Series_sample_id = GSM3202346 !Series_sample_id = GSM3202347 !Series_sample_id = GSM3202348 !Series_sample_id = GSM3202349 !Series_sample_id = GSM3202350 !Series_sample_id = GSM3202351 !Series_sample_id = GSM3202352 !Series_sample_id = GSM3202353 !Series_sample_id = GSM3202354 !Series_sample_id = GSM3202355 !Series_sample_id = GSM3202356 !Series_sample_id = GSM3202357 !Series_sample_id = GSM3202358 !Series_sample_id = GSM3202359 !Series_sample_id = GSM3202360 !Series_sample_id = GSM3202361 !Series_sample_id = GSM3202362 !Series_sample_id = GSM3202363 !Series_sample_id = GSM3202364 !Series_sample_id = GSM3202365 !Series_sample_id = GSM3202366 !Series_sample_id = GSM3202367 !Series_sample_id = GSM3202368 !Series_sample_id = GSM3202369 !Series_sample_id = GSM3202370 !Series_sample_id = GSM3202371 !Series_sample_id = GSM3202372 !Series_sample_id = GSM3202373 !Series_sample_id = GSM3202374 !Series_sample_id = GSM3202375 !Series_sample_id = GSM3202376 !Series_sample_id = GSM3202377 !Series_sample_id = GSM3202378 !Series_sample_id = GSM3202379 !Series_sample_id = GSM3202380 !Series_sample_id = GSM3202381 !Series_sample_id = GSM3202382 !Series_sample_id = GSM3202383 !Series_sample_id = GSM3202384 !Series_sample_id = GSM3202385 !Series_sample_id = GSM3202386 !Series_sample_id = GSM3202387 !Series_sample_id = GSM3202388 !Series_sample_id = GSM3202389 !Series_sample_id = GSM3202390 !Series_sample_id = GSM3202391 !Series_sample_id = GSM3202392 !Series_sample_id = GSM3202393 !Series_sample_id = GSM3202394 !Series_sample_id = GSM3202395 !Series_sample_id = GSM3202396 !Series_sample_id = GSM3202397 !Series_sample_id = GSM3202398 !Series_sample_id = GSM3202399 !Series_sample_id = GSM3202400 !Series_sample_id = GSM3202401 !Series_sample_id = GSM3202402 !Series_sample_id = GSM3202403 !Series_sample_id = GSM3202404 !Series_sample_id = GSM3202405 !Series_sample_id = GSM3202406 !Series_sample_id = GSM3202407 !Series_sample_id = GSM3202408 !Series_sample_id = GSM3202409 !Series_sample_id = GSM3202410 !Series_sample_id = GSM3202411 !Series_sample_id = GSM3202412 !Series_sample_id = GSM3202413 !Series_sample_id = GSM3202414 !Series_sample_id = GSM3202415 !Series_sample_id = GSM3202416 !Series_sample_id = GSM3202417 !Series_sample_id = GSM3202418 !Series_sample_id = GSM3202419 !Series_sample_id = GSM3202420 !Series_sample_id = GSM3202421 !Series_sample_id = GSM3202422 !Series_sample_id = GSM3202423 !Series_sample_id = GSM3202424 !Series_sample_id = GSM3202425 !Series_sample_id = GSM3202426 !Series_sample_id = GSM3202427 !Series_sample_id = GSM3202428 !Series_sample_id = GSM3202429 !Series_sample_id = GSM3202430 !Series_sample_id = GSM3202431 !Series_sample_id = GSM3202432 !Series_sample_id = GSM3202433 !Series_sample_id = GSM3202434 !Series_sample_id = GSM3202435 !Series_sample_id = GSM3202436 !Series_sample_id = GSM3202437 !Series_sample_id = GSM3202438 !Series_sample_id = GSM3202439 !Series_sample_id = GSM3202440 !Series_sample_id = GSM3202441 !Series_sample_id = GSM3202442 !Series_sample_id = GSM3202443 !Series_sample_id = GSM3202444 !Series_sample_id = GSM3202445 !Series_sample_id = GSM3202446 !Series_sample_id = GSM3202447 !Series_sample_id = GSM3202448 !Series_sample_id = GSM3202449 !Series_sample_id = GSM3202450 !Series_sample_id = GSM3202451 !Series_sample_id = GSM3202452 !Series_sample_id = GSM3202453 !Series_sample_id = GSM3202454 !Series_sample_id = GSM3202455 !Series_sample_id = GSM3202456 !Series_sample_id = GSM3202457 !Series_sample_id = GSM3202458 !Series_sample_id = GSM3202459 !Series_sample_id = GSM3202460 !Series_sample_id = GSM3202461 !Series_sample_id = GSM3202462 !Series_sample_id = GSM3202463 !Series_sample_id = GSM3202464 !Series_sample_id = GSM3202465 !Series_sample_id = GSM3202466 !Series_sample_id = GSM3202467 !Series_sample_id = GSM3202468 !Series_sample_id = GSM3202469 !Series_sample_id = GSM3202470 !Series_sample_id = GSM3202471 !Series_sample_id = GSM3202472 !Series_sample_id = GSM3202473 !Series_sample_id = GSM3202474 !Series_sample_id = GSM3202475 !Series_sample_id = GSM3202476 !Series_sample_id = GSM3202477 !Series_sample_id = GSM3202478 !Series_sample_id = GSM3202479 !Series_sample_id = GSM3202480 !Series_sample_id = GSM3202481 !Series_sample_id = GSM3202482 !Series_sample_id = GSM3202483 !Series_sample_id = GSM3202484 !Series_sample_id = GSM3202485 !Series_sample_id = GSM3202486 !Series_sample_id = GSM3202487 !Series_sample_id = GSM3202488 !Series_sample_id = GSM3202489 !Series_sample_id = GSM3202490 !Series_sample_id = GSM3202491 !Series_sample_id = GSM3202492 !Series_sample_id = GSM3202493 !Series_sample_id = GSM3202494 !Series_sample_id = GSM3202495 !Series_sample_id = GSM3202496 !Series_sample_id = GSM3202497 !Series_sample_id = GSM3202498 !Series_sample_id = GSM3202499 !Series_sample_id = GSM3202500 !Series_sample_id = GSM3202501 !Series_sample_id = GSM3202502 !Series_sample_id = GSM3202503 !Series_sample_id = GSM3202504 !Series_sample_id = GSM3202505 !Series_sample_id = GSM3202506 !Series_sample_id = GSM3202507 !Series_sample_id = GSM3202508 !Series_sample_id = GSM3202509 !Series_sample_id = GSM3202510 !Series_sample_id = GSM3202511 !Series_sample_id = GSM3202512 !Series_sample_id = GSM3202513 !Series_sample_id = GSM3202514 !Series_sample_id = GSM3202515 !Series_sample_id = GSM3202516 !Series_sample_id = GSM3202517 !Series_sample_id = GSM3202518 !Series_sample_id = GSM3202519 !Series_sample_id = GSM3202520 !Series_sample_id = GSM3202521 !Series_sample_id = GSM3202522 !Series_sample_id = GSM3202523 !Series_sample_id = GSM3202524 !Series_sample_id = GSM3202525 !Series_sample_id = GSM3202526 !Series_sample_id = GSM3202527 !Series_sample_id = GSM3202528 !Series_sample_id = GSM3202529 !Series_sample_id = GSM3202530 !Series_sample_id = GSM3202531 !Series_sample_id = GSM3202532 !Series_sample_id = GSM3202533 !Series_sample_id = GSM3202534 !Series_sample_id = GSM3202535 !Series_sample_id = GSM3202536 !Series_sample_id = GSM3202537 !Series_sample_id = GSM3202538 !Series_sample_id = GSM3202539 !Series_sample_id = GSM3202540 !Series_sample_id = GSM3202541 !Series_sample_id = GSM3202542 !Series_sample_id = GSM3202543 !Series_sample_id = GSM3202544 !Series_sample_id = GSM3202545 !Series_sample_id = GSM3202546 !Series_sample_id = GSM3202547 !Series_sample_id = GSM3202548 !Series_sample_id = GSM3202549 !Series_sample_id = GSM3202550 !Series_sample_id = GSM3202551 !Series_sample_id = GSM3202552 !Series_sample_id = GSM3202553 !Series_sample_id = GSM3202554 !Series_sample_id = GSM3202555 !Series_sample_id = GSM3202556 !Series_sample_id = GSM3202557 !Series_sample_id = GSM3202558 !Series_sample_id = GSM3202559 !Series_sample_id = GSM3202560 !Series_sample_id = GSM3202561 !Series_sample_id = GSM3202562 !Series_sample_id = GSM3202563 !Series_sample_id = GSM3202564 !Series_sample_id = GSM3202565 !Series_sample_id = GSM3202566 !Series_sample_id = GSM3202567 !Series_sample_id = GSM3202568 !Series_sample_id = GSM3202569 !Series_sample_id = GSM3202570 !Series_sample_id = GSM3202571 !Series_sample_id = GSM3202572 !Series_sample_id = GSM3202573 !Series_sample_id = GSM3202574 !Series_sample_id = GSM3202575 !Series_sample_id = GSM3202576 !Series_sample_id = GSM3202577 !Series_sample_id = GSM3202578 !Series_sample_id = GSM3202579 !Series_sample_id = GSM3202580 !Series_sample_id = GSM3202581 !Series_sample_id = GSM3202582 !Series_sample_id = GSM3202583 !Series_sample_id = GSM3202584 !Series_sample_id = GSM3202585 !Series_sample_id = GSM3202586 !Series_sample_id = GSM3202587 !Series_sample_id = GSM3202588 !Series_sample_id = GSM3202589 !Series_sample_id = GSM3202590 !Series_sample_id = GSM3202591 !Series_sample_id = GSM3202592 !Series_sample_id = GSM3202593 !Series_sample_id = GSM3202594 !Series_sample_id = GSM3202595 !Series_sample_id = GSM3202596 !Series_sample_id = GSM3202597 !Series_sample_id = GSM3202598 !Series_sample_id = GSM3202599 !Series_sample_id = GSM3202600 !Series_sample_id = GSM3202601 !Series_sample_id = GSM3202602 !Series_sample_id = GSM3202603 !Series_sample_id = GSM3202604 !Series_sample_id = GSM3202605 !Series_sample_id = GSM3202606 !Series_sample_id = GSM3202607 !Series_sample_id = GSM3202608 !Series_sample_id = GSM3202609 !Series_sample_id = GSM3202610 !Series_sample_id = GSM3202611 !Series_sample_id = GSM3202612 !Series_sample_id = GSM3202613 !Series_sample_id = GSM3202614 !Series_sample_id = GSM3202615 !Series_sample_id = GSM3202616 !Series_sample_id = GSM3202617 !Series_sample_id = GSM3202618 !Series_sample_id = GSM3202619 !Series_sample_id = GSM3202620 !Series_sample_id = GSM3202621 !Series_sample_id = GSM3202622 !Series_sample_id = GSM3202623 !Series_sample_id = GSM3202624 !Series_sample_id = GSM3202625 !Series_sample_id = GSM3202626 !Series_sample_id = GSM3202627 !Series_sample_id = GSM3202628 !Series_sample_id = GSM3202629 !Series_sample_id = GSM3202630 !Series_sample_id = GSM3202631 !Series_sample_id = GSM3202632 !Series_sample_id = GSM3202633 !Series_sample_id = GSM3202634 !Series_sample_id = GSM3202635 !Series_sample_id = GSM3202636 !Series_sample_id = GSM3202637 !Series_sample_id = GSM3202638 !Series_sample_id = GSM3202639 !Series_sample_id = GSM3202640 !Series_sample_id = GSM3202641 !Series_sample_id = GSM3202642 !Series_sample_id = GSM3202643 !Series_sample_id = GSM3202644 !Series_sample_id = GSM3202645 !Series_sample_id = GSM3202646 !Series_sample_id = GSM3202647 !Series_sample_id = GSM3202648 !Series_sample_id = GSM3202649 !Series_sample_id = GSM3202650 !Series_sample_id = GSM3202651 !Series_sample_id = GSM3202652 !Series_sample_id = GSM3202653 !Series_sample_id = GSM3202654 !Series_sample_id = GSM3202655 !Series_sample_id = GSM3202656 !Series_sample_id = GSM3202657 !Series_sample_id = GSM3202658 !Series_sample_id = GSM3202659 !Series_sample_id = GSM3202660 !Series_sample_id = GSM3202661 !Series_sample_id = GSM3202662 !Series_sample_id = GSM3202663 !Series_sample_id = GSM3202664 !Series_sample_id = GSM3202665 !Series_sample_id = GSM3202666 !Series_sample_id = GSM3202667 !Series_sample_id = GSM3202668 !Series_sample_id = GSM3202669 !Series_sample_id = GSM3202670 !Series_sample_id = GSM3202671 !Series_sample_id = GSM3202672 !Series_sample_id = GSM3202673 !Series_sample_id = GSM3202674 !Series_sample_id = GSM3202675 !Series_sample_id = GSM3202676 !Series_sample_id = GSM3202677 !Series_sample_id = GSM3202678 !Series_sample_id = GSM3202679 !Series_sample_id = GSM3202680 !Series_sample_id = GSM3202681 !Series_sample_id = GSM3202682 !Series_sample_id = GSM3202683 !Series_sample_id = GSM3202684 !Series_sample_id = GSM3202685 !Series_sample_id = GSM3202686 !Series_sample_id = GSM3202687 !Series_sample_id = GSM3202688 !Series_sample_id = GSM3202689 !Series_sample_id = GSM3202690 !Series_sample_id = GSM3202691 !Series_sample_id = GSM3202692 !Series_sample_id = GSM3202693 !Series_sample_id = GSM3202694 !Series_sample_id = GSM3202695 !Series_sample_id = GSM3202696 !Series_sample_id = GSM3202697 !Series_sample_id = GSM3202698 !Series_sample_id = GSM3202699 !Series_sample_id = GSM3202700 !Series_sample_id = GSM3202701 !Series_sample_id = GSM3202702 !Series_sample_id = GSM3202703 !Series_sample_id = GSM3202704 !Series_sample_id = GSM3202705 !Series_sample_id = GSM3202706 !Series_sample_id = GSM3202707 !Series_sample_id = GSM3202708 !Series_sample_id = GSM3202709 !Series_sample_id = GSM3202710 !Series_sample_id = GSM3202711 !Series_sample_id = GSM3202712 !Series_sample_id = GSM3202713 !Series_sample_id = GSM3202714 !Series_sample_id = GSM3202715 !Series_sample_id = GSM3202716 !Series_sample_id = GSM3202717 !Series_sample_id = GSM3202718 !Series_sample_id = GSM3202719 !Series_sample_id = GSM3202720 !Series_sample_id = GSM3202721 !Series_sample_id = GSM3202722 !Series_sample_id = GSM3202723 !Series_sample_id = GSM3202724 !Series_sample_id = GSM3202725 !Series_sample_id = GSM3202726 !Series_sample_id = GSM3202727 !Series_sample_id = GSM3202728 !Series_sample_id = GSM3202729 !Series_sample_id = GSM3202730 !Series_sample_id = GSM3202731 !Series_sample_id = GSM3202732 !Series_sample_id = GSM3202733 !Series_sample_id = GSM3202734 !Series_sample_id = GSM3202735 !Series_sample_id = GSM3202736 !Series_sample_id = GSM3202737 !Series_sample_id = GSM3202738 !Series_sample_id = GSM3202739 !Series_sample_id = GSM3202740 !Series_sample_id = GSM3202741 !Series_sample_id = GSM3202742 !Series_sample_id = GSM3202743 !Series_sample_id = GSM3202744 !Series_sample_id = GSM3202745 !Series_sample_id = GSM3202746 !Series_sample_id = GSM3202747 !Series_sample_id = GSM3202748 !Series_sample_id = GSM3202749 !Series_sample_id = GSM3202750 !Series_sample_id = GSM3202751 !Series_sample_id = GSM3202752 !Series_sample_id = GSM3202753 !Series_sample_id = GSM3202754 !Series_sample_id = GSM3202755 !Series_sample_id = GSM3202756 !Series_sample_id = GSM3202757 !Series_sample_id = GSM3202758 !Series_sample_id = GSM3202759 !Series_sample_id = GSM3202760 !Series_sample_id = GSM3202761 !Series_sample_id = GSM3202762 !Series_sample_id = GSM3202763 !Series_sample_id = GSM3202764 !Series_sample_id = GSM3202765 !Series_sample_id = GSM3202766 !Series_sample_id = GSM3202767 !Series_sample_id = GSM3202768 !Series_sample_id = GSM3202769 !Series_sample_id = GSM3202770 !Series_sample_id = GSM3202771 !Series_sample_id = GSM3202772 !Series_sample_id = GSM3202773 !Series_sample_id = GSM3202774 !Series_sample_id = GSM3202775 !Series_sample_id = GSM3202776 !Series_sample_id = GSM3202777 !Series_sample_id = GSM3202778 !Series_sample_id = GSM3202779 !Series_sample_id = GSM3202780 !Series_sample_id = GSM3202781 !Series_sample_id = GSM3202782 !Series_sample_id = GSM3202783 !Series_sample_id = GSM3202784 !Series_sample_id = GSM3202785 !Series_sample_id = GSM3202786 !Series_sample_id = GSM3202787 !Series_sample_id = GSM3202788 !Series_sample_id = GSM3202789 !Series_sample_id = GSM3202790 !Series_sample_id = GSM3202791 !Series_sample_id = GSM3202792 !Series_sample_id = GSM3202793 !Series_sample_id = GSM3202794 !Series_sample_id = GSM3202795 !Series_sample_id = GSM3202796 !Series_sample_id = GSM3202797 !Series_sample_id = GSM3202798 !Series_sample_id = GSM3202799 !Series_sample_id = GSM3202800 !Series_sample_id = GSM3202801 !Series_sample_id = GSM3202802 !Series_sample_id = GSM3202803 !Series_sample_id = GSM3202804 !Series_sample_id = GSM3202805 !Series_sample_id = GSM3202806 !Series_sample_id = GSM3202807 !Series_sample_id = GSM3202808 !Series_sample_id = GSM3202809 !Series_sample_id = GSM3202810 !Series_sample_id = GSM3202811 !Series_sample_id = GSM3202812 !Series_sample_id = GSM3202813 !Series_sample_id = GSM3202814 !Series_sample_id = GSM3202815 !Series_sample_id = GSM3202816 !Series_sample_id = GSM3202817 !Series_sample_id = GSM3202818 !Series_sample_id = GSM3202819 !Series_sample_id = GSM3202820 !Series_sample_id = GSM3202821 !Series_sample_id = GSM3202822 !Series_sample_id = GSM3202823 !Series_sample_id = GSM3202824 !Series_sample_id = GSM3202825 !Series_sample_id = GSM3202826 !Series_sample_id = GSM3202827 !Series_sample_id = GSM3202828 !Series_sample_id = GSM3202829 !Series_sample_id = GSM3202830 !Series_sample_id = GSM3202831 !Series_sample_id = GSM3202832 !Series_sample_id = GSM3202833 !Series_sample_id = GSM3202834 !Series_sample_id = GSM3202835 !Series_sample_id = GSM3202836 !Series_sample_id = GSM3202837 !Series_sample_id = GSM3202838 !Series_sample_id = GSM3202839 !Series_sample_id = GSM3202840 !Series_sample_id = GSM3202841 !Series_sample_id = GSM3202842 !Series_sample_id = GSM3202843 !Series_sample_id = GSM3202844 !Series_sample_id = GSM3202845 !Series_sample_id = GSM3202846 !Series_sample_id = GSM3202847 !Series_sample_id = GSM3202848 !Series_sample_id = GSM3202849 !Series_sample_id = GSM3202850 !Series_sample_id = GSM3202851 !Series_sample_id = GSM3202852 !Series_sample_id = GSM3202853 !Series_sample_id = GSM3202854 !Series_sample_id = GSM3202855 !Series_sample_id = GSM3202856 !Series_sample_id = GSM3202857 !Series_sample_id = GSM3202858 !Series_sample_id = GSM3202859 !Series_sample_id = GSM3202860 !Series_sample_id = GSM3202861 !Series_sample_id = GSM3202862 !Series_sample_id = GSM3202863 !Series_sample_id = GSM3202864 !Series_sample_id = GSM3202865 !Series_sample_id = GSM3202866 !Series_sample_id = GSM3202867 !Series_sample_id = GSM3202868 !Series_sample_id = GSM3202869 !Series_sample_id = GSM3202870 !Series_sample_id = GSM3202871 !Series_sample_id = GSM3202872 !Series_sample_id = GSM3202873 !Series_sample_id = GSM3202874 !Series_sample_id = GSM3202875 !Series_sample_id = GSM3202876 !Series_sample_id = GSM3202877 !Series_sample_id = GSM3202878 !Series_sample_id = GSM3202879 !Series_sample_id = GSM3202880 !Series_sample_id = GSM3202881 !Series_sample_id = GSM3202882 !Series_sample_id = GSM3202883 !Series_sample_id = GSM3202884 !Series_sample_id = GSM3202885 !Series_sample_id = GSM3202886 !Series_sample_id = GSM3202887 !Series_sample_id = GSM3202888 !Series_sample_id = GSM3202889 !Series_sample_id = GSM3202890 !Series_sample_id = GSM3202891 !Series_sample_id = GSM3202892 !Series_sample_id = GSM3202893 !Series_sample_id = GSM3202894 !Series_sample_id = GSM3202895 !Series_sample_id = GSM3202896 !Series_sample_id = GSM3202897 !Series_sample_id = GSM3202898 !Series_sample_id = GSM3202899 !Series_sample_id = GSM3202900 !Series_sample_id = GSM3202901 !Series_sample_id = GSM3202902 !Series_sample_id = GSM3202903 !Series_sample_id = GSM3202904 !Series_sample_id = GSM3202905 !Series_sample_id = GSM3202906 !Series_sample_id = GSM3202907 !Series_sample_id = GSM3202908 !Series_sample_id = GSM3202909 !Series_sample_id = GSM3202910 !Series_sample_id = GSM3202911 !Series_sample_id = GSM3202912 !Series_sample_id = GSM3202913 !Series_sample_id = GSM3202914 !Series_sample_id = GSM3202915 !Series_sample_id = GSM3202916 !Series_sample_id = GSM3202917 !Series_sample_id = GSM3202918 !Series_sample_id = GSM3202919 !Series_sample_id = GSM3202920 !Series_sample_id = GSM3202921 !Series_sample_id = GSM3202922 !Series_sample_id = GSM3202923 !Series_sample_id = GSM3202924 !Series_sample_id = GSM3202925 !Series_sample_id = GSM3202926 !Series_sample_id = GSM3202927 !Series_sample_id = GSM3202928 !Series_sample_id = GSM3202929 !Series_sample_id = GSM3202930 !Series_sample_id = GSM3202931 !Series_sample_id = GSM3202932 !Series_sample_id = GSM3202933 !Series_sample_id = GSM3202934 !Series_sample_id = GSM3202935 !Series_sample_id = GSM3202936 !Series_sample_id = GSM3202937 !Series_sample_id = GSM3202938 !Series_sample_id = GSM3202939 !Series_sample_id = GSM3202940 !Series_sample_id = GSM3202941 !Series_sample_id = GSM3202942 !Series_sample_id = GSM3202943 !Series_sample_id = GSM3202944 !Series_sample_id = GSM3202945 !Series_sample_id = GSM3202946 !Series_sample_id = GSM3202947 !Series_sample_id = GSM3202948 !Series_sample_id = GSM3202949 !Series_sample_id = GSM3202950 !Series_sample_id = GSM3202951 !Series_sample_id = GSM3202952 !Series_sample_id = GSM3202953 !Series_sample_id = GSM3202954 !Series_sample_id = GSM3202955 !Series_sample_id = GSM3202956 !Series_sample_id = GSM3202957 !Series_sample_id = GSM3202958 !Series_sample_id = GSM3202959 !Series_sample_id = GSM3202960 !Series_sample_id = GSM3202961 !Series_sample_id = GSM3202962 !Series_sample_id = GSM3202963 !Series_sample_id = GSM3202964 !Series_sample_id = GSM3202965 !Series_sample_id = GSM3202966 !Series_sample_id = GSM3202967 !Series_sample_id = GSM3202968 !Series_sample_id = GSM3202969 !Series_sample_id = GSM3202970 !Series_sample_id = GSM3202971 !Series_sample_id = GSM3202972 !Series_sample_id = GSM3202973 !Series_sample_id = GSM3202974 !Series_sample_id = GSM3202975 !Series_sample_id = GSM3202976 !Series_sample_id = GSM3202977 !Series_sample_id = GSM3202978 !Series_sample_id = GSM3202979 !Series_sample_id = GSM3202980 !Series_sample_id = GSM3202981 !Series_sample_id = GSM3202982 !Series_sample_id = GSM3202983 !Series_sample_id = GSM3202984 !Series_sample_id = GSM3202985 !Series_sample_id = GSM3202986 !Series_sample_id = GSM3202987 !Series_sample_id = GSM3202988 !Series_sample_id = GSM3202989 !Series_sample_id = GSM3202990 !Series_sample_id = GSM3202991 !Series_sample_id = GSM3202992 !Series_sample_id = GSM3202993 !Series_sample_id = GSM3202994 !Series_sample_id = GSM3202995 !Series_sample_id = GSM3202996 !Series_sample_id = GSM3202997 !Series_sample_id = GSM3202998 !Series_sample_id = GSM3202999 !Series_sample_id = GSM3203000 !Series_sample_id = GSM3203001 !Series_sample_id = GSM3203002 !Series_sample_id = GSM3203003 !Series_sample_id = GSM3203004 !Series_sample_id = GSM3203005 !Series_sample_id = GSM3203006 !Series_sample_id = GSM3203007 !Series_sample_id = GSM3203008 !Series_sample_id = GSM3203009 !Series_sample_id = GSM3203010 !Series_sample_id = GSM3203011 !Series_sample_id = GSM3203012 !Series_sample_id = GSM3203013 !Series_sample_id = GSM3203014 !Series_sample_id = GSM3203015 !Series_sample_id = GSM3203016 !Series_sample_id = GSM3203017 !Series_sample_id = GSM3203018 !Series_sample_id = GSM3203019 !Series_sample_id = GSM3203020 !Series_sample_id = GSM3203021 !Series_sample_id = GSM3203022 !Series_sample_id = GSM3203023 !Series_sample_id = GSM3203024 !Series_sample_id = GSM3203025 !Series_sample_id = GSM3203026 !Series_sample_id = GSM3203027 !Series_sample_id = GSM3203028 !Series_sample_id = GSM3203029 !Series_sample_id = GSM3203030 !Series_sample_id = GSM3203031 !Series_sample_id = GSM3203032 !Series_sample_id = GSM3203033 !Series_sample_id = GSM3203034 !Series_sample_id = GSM3203035 !Series_sample_id = GSM3203036 !Series_sample_id = GSM3203037 !Series_sample_id = GSM3203038 !Series_sample_id = GSM3203039 !Series_sample_id = GSM3203040 !Series_sample_id = GSM3203041 !Series_sample_id = GSM3203042 !Series_sample_id = GSM3203043 !Series_sample_id = GSM3203044 !Series_sample_id = GSM3203045 !Series_sample_id = GSM3203046 !Series_sample_id = GSM3203047 !Series_sample_id = GSM3203048 !Series_sample_id = GSM3203049 !Series_sample_id = GSM3203050 !Series_sample_id = GSM3203051 !Series_sample_id = GSM3203052 !Series_sample_id = GSM3203053 !Series_sample_id = GSM3203054 !Series_sample_id = GSM3203055 !Series_sample_id = GSM3203056 !Series_sample_id = GSM3203057 !Series_sample_id = GSM3203058 !Series_sample_id = GSM3203059 !Series_sample_id = GSM3203060 !Series_sample_id = GSM3203061 !Series_sample_id = GSM3203062 !Series_sample_id = GSM3203063 !Series_sample_id = GSM3203064 !Series_sample_id = GSM3203065 !Series_sample_id = GSM3203066 !Series_sample_id = GSM3203067 !Series_sample_id = GSM3203068 !Series_sample_id = GSM3203069 !Series_sample_id = GSM3203070 !Series_sample_id = GSM3203071 !Series_sample_id = GSM3203072 !Series_sample_id = GSM3203073 !Series_sample_id = GSM3203074 !Series_sample_id = GSM3203075 !Series_sample_id = GSM3203076 !Series_sample_id = GSM3203077 !Series_sample_id = GSM3203078 !Series_sample_id = GSM3203079 !Series_sample_id = GSM3203080 !Series_sample_id = GSM3203081 !Series_sample_id = GSM3203082 !Series_sample_id = GSM3203083 !Series_sample_id = GSM3203084 !Series_sample_id = GSM3203085 !Series_sample_id = GSM3203086 !Series_sample_id = GSM3203087 !Series_sample_id = GSM3203088 !Series_sample_id = GSM3203089 !Series_sample_id = GSM3203090 !Series_sample_id = GSM3203091 !Series_sample_id = GSM3203092 !Series_sample_id = GSM3203093 !Series_sample_id = GSM3203094 !Series_sample_id = GSM3203095 !Series_sample_id = GSM3203096 !Series_sample_id = GSM3203097 !Series_sample_id = GSM3203098 !Series_sample_id = GSM3203099 !Series_sample_id = GSM3203100 !Series_sample_id = GSM3203101 !Series_sample_id = GSM3203102 !Series_sample_id = GSM3203103 !Series_sample_id = GSM3203104 !Series_sample_id = GSM3203105 !Series_sample_id = GSM3203106 !Series_sample_id = GSM3203107 !Series_sample_id = GSM3203108 !Series_sample_id = GSM3203109 !Series_sample_id = GSM3203110 !Series_sample_id = GSM3203111 !Series_sample_id = GSM3203112 !Series_sample_id = GSM3203113 !Series_sample_id = GSM3203114 !Series_sample_id = GSM3203115 !Series_sample_id = GSM3203116 !Series_sample_id = GSM3203117 !Series_sample_id = GSM3203118 !Series_sample_id = GSM3203119 !Series_sample_id = GSM3203120 !Series_sample_id = GSM3203121 !Series_sample_id = GSM3203122 !Series_sample_id = GSM3203123 !Series_sample_id = GSM3203124 !Series_sample_id = GSM3203125 !Series_sample_id = GSM3203126 !Series_sample_id = GSM3203127 !Series_sample_id = GSM3203128 !Series_sample_id = GSM3203129 !Series_sample_id = GSM3203130 !Series_sample_id = GSM3203131 !Series_sample_id = GSM3203132 !Series_sample_id = GSM3203133 !Series_sample_id = GSM3203134 !Series_sample_id = GSM3203135 !Series_sample_id = GSM3203136 !Series_sample_id = GSM3203137 !Series_sample_id = GSM3203138 !Series_sample_id = GSM3203139 !Series_sample_id = GSM3203140 !Series_sample_id = GSM3203141 !Series_sample_id = GSM3203142 !Series_sample_id = GSM3203143 !Series_sample_id = GSM3203144 !Series_sample_id = GSM3203145 !Series_sample_id = GSM3203146 !Series_sample_id = GSM3203147 !Series_sample_id = GSM3203148 !Series_sample_id = GSM3203149 !Series_sample_id = GSM3203150 !Series_sample_id = GSM3203151 !Series_sample_id = GSM3203152 !Series_sample_id = GSM3203153 !Series_sample_id = GSM3203154 !Series_sample_id = GSM3203155 !Series_sample_id = GSM3203156 !Series_sample_id = GSM3203157 !Series_sample_id = GSM3203158 !Series_sample_id = GSM3203159 !Series_sample_id = GSM3203160 !Series_sample_id = GSM3203161 !Series_sample_id = GSM3203162 !Series_sample_id = GSM3203163 !Series_sample_id = GSM3203164 !Series_sample_id = GSM3203165 !Series_sample_id = GSM3203166 !Series_sample_id = GSM3203167 !Series_sample_id = GSM3203168 !Series_sample_id = GSM3203169 !Series_sample_id = GSM3203170 !Series_sample_id = GSM3203171 !Series_sample_id = GSM3203172 !Series_sample_id = GSM3203173 !Series_sample_id = GSM3203174 !Series_sample_id = GSM3203175 !Series_sample_id = GSM3203176 !Series_sample_id = GSM3203177 !Series_sample_id = GSM3203178 !Series_sample_id = GSM3203179 !Series_sample_id = GSM3203180 !Series_sample_id = GSM3203181 !Series_sample_id = GSM3203182 !Series_sample_id = GSM3203183 !Series_sample_id = GSM3203184 !Series_sample_id = GSM3203185 !Series_sample_id = GSM3203186 !Series_sample_id = GSM3203187 !Series_sample_id = GSM3203188 !Series_sample_id = GSM3203189 !Series_sample_id = GSM3203190 !Series_sample_id = GSM3203191 !Series_sample_id = GSM3203192 !Series_sample_id = GSM3203193 !Series_sample_id = GSM3203194 !Series_sample_id = GSM3203195 !Series_sample_id = GSM3203196 !Series_sample_id = GSM3203197 !Series_sample_id = GSM3203198 !Series_sample_id = GSM3203199 !Series_sample_id = GSM3203200 !Series_sample_id = GSM3203201 !Series_sample_id = GSM3203202 !Series_sample_id = GSM3203203 !Series_sample_id = GSM3203204 !Series_sample_id = GSM3203205 !Series_sample_id = GSM3203206 !Series_sample_id = GSM3203207 !Series_sample_id = GSM3203208 !Series_sample_id = GSM3203209 !Series_sample_id = GSM3203210 !Series_sample_id = GSM3203211 !Series_sample_id = GSM3203212 !Series_sample_id = GSM3203213 !Series_sample_id = GSM3203214 !Series_sample_id = GSM3203215 !Series_sample_id = GSM3203216 !Series_sample_id = GSM3203217 !Series_sample_id = GSM3203218 !Series_sample_id = GSM3203219 !Series_sample_id = GSM3203220 !Series_sample_id = GSM3203221 !Series_sample_id = GSM3203222 !Series_sample_id = GSM3203223 !Series_sample_id = GSM3203224 !Series_sample_id = GSM3203225 !Series_sample_id = GSM3203226 !Series_sample_id = GSM3203227 !Series_sample_id = GSM3203228 !Series_sample_id = GSM3203229 !Series_sample_id = GSM3203230 !Series_sample_id = GSM3203231 !Series_sample_id = GSM3203232 !Series_sample_id = GSM3203233 !Series_sample_id = GSM3203234 !Series_sample_id = GSM3203235 !Series_sample_id = GSM3203236 !Series_sample_id = GSM3203237 !Series_sample_id = GSM3203238 !Series_sample_id = GSM3203239 !Series_sample_id = GSM3203240 !Series_sample_id = GSM3203241 !Series_sample_id = GSM3203242 !Series_sample_id = GSM3203243 !Series_sample_id = GSM3203244 !Series_sample_id = GSM3203245 !Series_sample_id = GSM3203246 !Series_sample_id = GSM3203247 !Series_sample_id = GSM3203248 !Series_sample_id = GSM3203249 !Series_sample_id = GSM3203250 !Series_sample_id = GSM3203251 !Series_sample_id = GSM3203252 !Series_sample_id = GSM3203253 !Series_sample_id = GSM3203254 !Series_sample_id = GSM3203255 !Series_sample_id = GSM3203256 !Series_sample_id = GSM3203257 !Series_sample_id = GSM3203258 !Series_sample_id = GSM3203259 !Series_sample_id = GSM3203260 !Series_sample_id = GSM3203261 !Series_sample_id = GSM3203262 !Series_sample_id = GSM3203263 !Series_sample_id = GSM3203264 !Series_sample_id = GSM3203265 !Series_sample_id = GSM3203266 !Series_sample_id = GSM3203267 !Series_sample_id = GSM3203268 !Series_sample_id = GSM3203269 !Series_sample_id = GSM3203270 !Series_sample_id = GSM3203271 !Series_sample_id = GSM3203272 !Series_sample_id = GSM3203273 !Series_sample_id = GSM3203274 !Series_sample_id = GSM3203275 !Series_sample_id = GSM3203276 !Series_sample_id = GSM3203277 !Series_sample_id = GSM3203278 !Series_sample_id = GSM3203279 !Series_sample_id = GSM3203280 !Series_sample_id = GSM3203281 !Series_sample_id = GSM3203282 !Series_sample_id = GSM3203283 !Series_sample_id = GSM3203284 !Series_sample_id = GSM3203285 !Series_sample_id = GSM3203286 !Series_sample_id = GSM3203287 !Series_sample_id = GSM3203288 !Series_sample_id = GSM3203289 !Series_sample_id = GSM3203290 !Series_sample_id = GSM3203291 !Series_sample_id = GSM3203292 !Series_sample_id = GSM3203293 !Series_sample_id = GSM3203294 !Series_sample_id = GSM3203295 !Series_sample_id = GSM3203296 !Series_sample_id = GSM3203297 !Series_sample_id = GSM3203298 !Series_sample_id = GSM3203299 !Series_sample_id = GSM3203300 !Series_sample_id = GSM3203301 !Series_sample_id = GSM3203302 !Series_sample_id = GSM3203303 !Series_sample_id = GSM3203304 !Series_sample_id = GSM3203305 !Series_sample_id = GSM3203306 !Series_sample_id = GSM3203307 !Series_sample_id = GSM3203308 !Series_sample_id = GSM3203309 !Series_sample_id = GSM3203310 !Series_sample_id = GSM3203311 !Series_sample_id = GSM3203312 !Series_sample_id = GSM3203313 !Series_sample_id = GSM3203314 !Series_sample_id = GSM3203315 !Series_sample_id = GSM3203316 !Series_sample_id = GSM3203317 !Series_sample_id = GSM3203318 !Series_sample_id = GSM3203319 !Series_sample_id = GSM3203320 !Series_sample_id = GSM3203321 !Series_sample_id = GSM3203322 !Series_sample_id = GSM3203323 !Series_sample_id = GSM3203324 !Series_sample_id = GSM3203325 !Series_sample_id = GSM3203326 !Series_sample_id = GSM3203327 !Series_sample_id = GSM3203328 !Series_sample_id = GSM3203329 !Series_sample_id = GSM3203330 !Series_sample_id = GSM3203331 !Series_sample_id = GSM3203332 !Series_sample_id = GSM3203333 !Series_sample_id = GSM3203334 !Series_sample_id = GSM3203335 !Series_sample_id = GSM3203336 !Series_sample_id = GSM3203337 !Series_sample_id = GSM3203338 !Series_sample_id = GSM3203339 !Series_sample_id = GSM3203340 !Series_sample_id = GSM3203341 !Series_sample_id = GSM3203342 !Series_sample_id = GSM3203343 !Series_sample_id = GSM3203344 !Series_sample_id = GSM3203345 !Series_sample_id = GSM3203346 !Series_sample_id = GSM3203347 !Series_sample_id = GSM3203348 !Series_sample_id = GSM3203349 !Series_sample_id = GSM3203350 !Series_sample_id = GSM3203351 !Series_sample_id = GSM3203352 !Series_sample_id = GSM3203353 !Series_sample_id = GSM3203354 !Series_sample_id = GSM3203355 !Series_sample_id = GSM3203356 !Series_sample_id = GSM3203357 !Series_sample_id = GSM3203358 !Series_sample_id = GSM3203359 !Series_sample_id = GSM3203360 !Series_sample_id = GSM3203361 !Series_sample_id = GSM3203362 !Series_sample_id = GSM3203363 !Series_sample_id = GSM3203364 !Series_sample_id = GSM3203365 !Series_sample_id = GSM3203366 !Series_sample_id = GSM3203367 !Series_sample_id = GSM3203368 !Series_sample_id = GSM3203369 !Series_sample_id = GSM3203370 !Series_sample_id = GSM3203371 !Series_sample_id = GSM3203372 !Series_sample_id = GSM3203373 !Series_sample_id = GSM3203374 !Series_sample_id = GSM3203375 !Series_sample_id = GSM3203376 !Series_sample_id = GSM3203377 !Series_sample_id = GSM3203378 !Series_sample_id = GSM3203379 !Series_sample_id = GSM3203380 !Series_sample_id = GSM3203381 !Series_sample_id = GSM3203382 !Series_sample_id = GSM3203383 !Series_sample_id = GSM3203384 !Series_sample_id = GSM3203385 !Series_sample_id = GSM3203386 !Series_sample_id = GSM3203387 !Series_sample_id = GSM3203388 !Series_sample_id = GSM3203389 !Series_sample_id = GSM3203390 !Series_sample_id = GSM3203391 !Series_sample_id = GSM3203392 !Series_sample_id = GSM3203393 !Series_sample_id = GSM3203394 !Series_sample_id = GSM3203395 !Series_sample_id = GSM3203396 !Series_sample_id = GSM3203397 !Series_sample_id = GSM3203398 !Series_sample_id = GSM3203399 !Series_sample_id = GSM3203400 !Series_sample_id = GSM3203401 !Series_sample_id = GSM3203402 !Series_sample_id = GSM3203403 !Series_sample_id = GSM3203404 !Series_sample_id = GSM3203405 !Series_sample_id = GSM3203406 !Series_sample_id = GSM3203407 !Series_sample_id = GSM3203408 !Series_sample_id = GSM3203409 !Series_sample_id = GSM3203410 !Series_sample_id = GSM3203411 !Series_sample_id = GSM3203412 !Series_sample_id = GSM3203413 !Series_sample_id = GSM3203414 !Series_sample_id = GSM3203415 !Series_sample_id = GSM3203416 !Series_sample_id = GSM3203417 !Series_sample_id = GSM3203418 !Series_sample_id = GSM3203419 !Series_sample_id = GSM3203420 !Series_sample_id = GSM3203421 !Series_sample_id = GSM3203422 !Series_sample_id = GSM3203423 !Series_sample_id = GSM3203424 !Series_sample_id = GSM3203425 !Series_sample_id = GSM3203426 !Series_sample_id = GSM3203427 !Series_sample_id = GSM3203428 !Series_sample_id = GSM3203429 !Series_sample_id = GSM3203430 !Series_sample_id = GSM3203431 !Series_sample_id = GSM3203432 !Series_sample_id = GSM3203433 !Series_sample_id = GSM3203434 !Series_sample_id = GSM3203435 !Series_sample_id = GSM3203436 !Series_sample_id = GSM3203437 !Series_sample_id = GSM3203438 !Series_sample_id = GSM3203439 !Series_sample_id = GSM3203440 !Series_sample_id = GSM3203441 !Series_sample_id = GSM3203442 !Series_sample_id = GSM3203443 !Series_sample_id = GSM3203444 !Series_sample_id = GSM3203445 !Series_sample_id = GSM3203446 !Series_sample_id = GSM3203447 !Series_sample_id = GSM3203448 !Series_sample_id = GSM3203449 !Series_sample_id = GSM3203450 !Series_sample_id = GSM3203451 !Series_sample_id = GSM3203452 !Series_sample_id = GSM3203453 !Series_sample_id = GSM3203454 !Series_sample_id = GSM3203455 !Series_sample_id = GSM3203456 !Series_sample_id = GSM3203457 !Series_sample_id = GSM3203458 !Series_sample_id = GSM3203459 !Series_sample_id = GSM3203460 !Series_sample_id = GSM3203461 !Series_sample_id = GSM3203462 !Series_sample_id = GSM3203463 !Series_sample_id = GSM3203464 !Series_sample_id = GSM3203465 !Series_sample_id = GSM3203466 !Series_sample_id = GSM3203467 !Series_sample_id = GSM3203468 !Series_sample_id = GSM3203469 !Series_sample_id = GSM3203470 !Series_sample_id = GSM3203471 !Series_sample_id = GSM3203472 !Series_sample_id = GSM3203473 !Series_sample_id = GSM3203474 !Series_sample_id = GSM3203475 !Series_sample_id = GSM3203476 !Series_sample_id = GSM3203477 !Series_sample_id = GSM3203478 !Series_sample_id = GSM3203479 !Series_sample_id = GSM3203480 !Series_sample_id = GSM3203481 !Series_sample_id = GSM3203482 !Series_sample_id = GSM3203483 !Series_sample_id = GSM3203484 !Series_sample_id = GSM3203485 !Series_sample_id = GSM3203486 !Series_sample_id = GSM3203487 !Series_sample_id = GSM3203488 !Series_sample_id = GSM3203489 !Series_sample_id = GSM3203490 !Series_sample_id = GSM3203491 !Series_sample_id = GSM3203492 !Series_sample_id = GSM3203493 !Series_sample_id = GSM3203494 !Series_sample_id = GSM3203495 !Series_sample_id = GSM3203496 !Series_sample_id = GSM3203497 !Series_sample_id = GSM3203498 !Series_sample_id = GSM3203499 !Series_sample_id = GSM3203500 !Series_sample_id = GSM3203501 !Series_sample_id = GSM3203502 !Series_sample_id = GSM3203503 !Series_sample_id = GSM3203504 !Series_sample_id = GSM3203505 !Series_sample_id = GSM3203506 !Series_sample_id = GSM3203507 !Series_sample_id = GSM3203508 !Series_sample_id = GSM3203509 !Series_sample_id = GSM3203510 !Series_sample_id = GSM3203511 !Series_sample_id = GSM3203512 !Series_sample_id = GSM3203513 !Series_sample_id = GSM3203514 !Series_sample_id = GSM3203515 !Series_sample_id = GSM3203516 !Series_sample_id = GSM3203517 !Series_sample_id = GSM3203518 !Series_sample_id = GSM3203519 !Series_sample_id = GSM3203520 !Series_sample_id = GSM3203521 !Series_sample_id = GSM3203522 !Series_sample_id = GSM3203523 !Series_sample_id = GSM3203524 !Series_sample_id = GSM3203525 !Series_sample_id = GSM3203526 !Series_sample_id = GSM3203527 !Series_sample_id = GSM3203528 !Series_sample_id = GSM3203529 !Series_sample_id = GSM3203530 !Series_sample_id = GSM3203531 !Series_sample_id = GSM3203532 !Series_sample_id = GSM3203533 !Series_sample_id = GSM3203534 !Series_sample_id = GSM3203535 !Series_sample_id = GSM3203536 !Series_sample_id = GSM3203537 !Series_sample_id = GSM3203538 !Series_sample_id = GSM3203539 !Series_sample_id = GSM3203540 !Series_sample_id = GSM3203541 !Series_sample_id = GSM3203542 !Series_sample_id = GSM3203543 !Series_sample_id = GSM3203544 !Series_sample_id = GSM3203545 !Series_sample_id = GSM3203546 !Series_sample_id = GSM3203547 !Series_sample_id = GSM3203548 !Series_sample_id = GSM3203549 !Series_sample_id = GSM3203550 !Series_sample_id = GSM3203551 !Series_sample_id = GSM3203552 !Series_sample_id = GSM3203553 !Series_sample_id = GSM3203554 !Series_sample_id = GSM3203555 !Series_sample_id = GSM3203556 !Series_sample_id = GSM3203557 !Series_sample_id = GSM3203558 !Series_sample_id = GSM3203559 !Series_sample_id = GSM3203560 !Series_sample_id = GSM3203561 !Series_sample_id = GSM3203562 !Series_sample_id = GSM3203563 !Series_sample_id = GSM3203564 !Series_sample_id = GSM3203565 !Series_sample_id = GSM3203566 !Series_sample_id = GSM3203567 !Series_sample_id = GSM3203568 !Series_sample_id = GSM3203569 !Series_sample_id = GSM3203570 !Series_sample_id = GSM3203571 !Series_sample_id = GSM3203572 !Series_sample_id = GSM3203573 !Series_sample_id = GSM3203574 !Series_sample_id = GSM3203575 !Series_sample_id = GSM3203576 !Series_sample_id = GSM3203577 !Series_sample_id = GSM3203578 !Series_sample_id = GSM3203579 !Series_sample_id = GSM3203580 !Series_sample_id = GSM3203581 !Series_sample_id = GSM3203582 !Series_sample_id = GSM3203583 !Series_sample_id = GSM3203584 !Series_sample_id = GSM3203585 !Series_sample_id = GSM3203586 !Series_sample_id = GSM3203587 !Series_sample_id = GSM3203588 !Series_sample_id = GSM3203589 !Series_sample_id = GSM3203590 !Series_sample_id = GSM3203591 !Series_sample_id = GSM3203592 !Series_sample_id = GSM3203593 !Series_sample_id = GSM3203594 !Series_sample_id = GSM3203595 !Series_sample_id = GSM3203596 !Series_sample_id = GSM3203597 !Series_sample_id = GSM3203598 !Series_sample_id = GSM3203599 !Series_sample_id = GSM3203600 !Series_sample_id = GSM3203601 !Series_sample_id = GSM3203602 !Series_sample_id = GSM3203603 !Series_sample_id = GSM3203604 !Series_sample_id = GSM3203605 !Series_sample_id = GSM3203606 !Series_sample_id = GSM3203607 !Series_sample_id = GSM3203608 !Series_sample_id = GSM3203609 !Series_sample_id = GSM3203610 !Series_sample_id = GSM3203611 !Series_sample_id = GSM3203612 !Series_sample_id = GSM3203613 !Series_sample_id = GSM3203614 !Series_sample_id = GSM3203615 !Series_sample_id = GSM3203616 !Series_sample_id = GSM3203617 !Series_sample_id = GSM3203618 !Series_sample_id = GSM3203619 !Series_sample_id = GSM3203620 !Series_sample_id = GSM3203621 !Series_sample_id = GSM3203622 !Series_sample_id = GSM3203623 !Series_sample_id = GSM3203624 !Series_sample_id = GSM3203625 !Series_sample_id = GSM3203626 !Series_sample_id = GSM3203627 !Series_sample_id = GSM3203628 !Series_sample_id = GSM3203629 !Series_sample_id = GSM3203630 !Series_sample_id = GSM3203631 !Series_sample_id = GSM3203632 !Series_sample_id = GSM3203633 !Series_sample_id = GSM3203634 !Series_sample_id = GSM3203635 !Series_sample_id = GSM3203636 !Series_sample_id = GSM3203637 !Series_sample_id = GSM3203638 !Series_sample_id = GSM3203639 !Series_sample_id = GSM3203640 !Series_sample_id = GSM3203641 !Series_sample_id = GSM3203642 !Series_sample_id = GSM3203643 !Series_sample_id = GSM3203644 !Series_sample_id = GSM3203645 !Series_sample_id = GSM3203646 !Series_sample_id = GSM3203647 !Series_sample_id = GSM3203648 !Series_sample_id = GSM3203649 !Series_sample_id = GSM3203650 !Series_sample_id = GSM3203651 !Series_sample_id = GSM3203652 !Series_sample_id = GSM3203653 !Series_sample_id = GSM3203654 !Series_sample_id = GSM3203655 !Series_sample_id = GSM3203656 !Series_sample_id = GSM3203657 !Series_sample_id = GSM3203658 !Series_sample_id = GSM3203659 !Series_sample_id = GSM3203660 !Series_sample_id = GSM3203661 !Series_sample_id = GSM3203662 !Series_sample_id = GSM3203663 !Series_sample_id = GSM3203664 !Series_sample_id = GSM3203665 !Series_sample_id = GSM3203666 !Series_sample_id = GSM3203667 !Series_sample_id = GSM3203668 !Series_sample_id = GSM3203669 !Series_sample_id = GSM3203670 !Series_sample_id = GSM3203671 !Series_sample_id = GSM3203672 !Series_sample_id = GSM3203673 !Series_sample_id = GSM3203674 !Series_sample_id = GSM3203675 !Series_sample_id = GSM3203676 !Series_sample_id = GSM3203677 !Series_sample_id = GSM3203678 !Series_sample_id = GSM3203679 !Series_sample_id = GSM3203680 !Series_sample_id = GSM3203681 !Series_sample_id = GSM3203682 !Series_sample_id = GSM3203683 !Series_sample_id = GSM3203684 !Series_sample_id = GSM3203685 !Series_sample_id = GSM3203686 !Series_sample_id = GSM3203687 !Series_sample_id = GSM3203688 !Series_sample_id = GSM3203689 !Series_sample_id = GSM3203690 !Series_sample_id = GSM3203691 !Series_sample_id = GSM3203692 !Series_sample_id = GSM3203693 !Series_sample_id = GSM3203694 !Series_sample_id = GSM3203695 !Series_sample_id = GSM3203696 !Series_sample_id = GSM3203697 !Series_sample_id = GSM3203698 !Series_sample_id = GSM3203699 !Series_sample_id = GSM3203700 !Series_sample_id = GSM3203701 !Series_sample_id = GSM3203702 !Series_sample_id = GSM3203703 !Series_sample_id = GSM3203704 !Series_sample_id = GSM3203705 !Series_sample_id = GSM3203706 !Series_sample_id = GSM3203707 !Series_sample_id = GSM3203708 !Series_sample_id = GSM3203709 !Series_sample_id = GSM3203710 !Series_sample_id = GSM3203711 !Series_sample_id = GSM3203712 !Series_sample_id = GSM3203713 !Series_sample_id = GSM3203714 !Series_sample_id = GSM3203715 !Series_sample_id = GSM3203716 !Series_sample_id = GSM3203717 !Series_sample_id = GSM3203718 !Series_sample_id = GSM3203719 !Series_sample_id = GSM3203720 !Series_sample_id = GSM3203721 !Series_sample_id = GSM3203722 !Series_sample_id = GSM3203723 !Series_sample_id = GSM3203724 !Series_sample_id = GSM3203725 !Series_sample_id = GSM3203726 !Series_sample_id = GSM3203727 !Series_sample_id = GSM3203728 !Series_sample_id = GSM3203729 !Series_sample_id = GSM3203730 !Series_sample_id = GSM3203731 !Series_sample_id = GSM3203732 !Series_sample_id = GSM3203733 !Series_sample_id = GSM3203734 !Series_sample_id = GSM3203735 !Series_sample_id = GSM3203736 !Series_sample_id = GSM3203737 !Series_sample_id = GSM3203738 !Series_sample_id = GSM3203739 !Series_sample_id = GSM3203740 !Series_sample_id = GSM3203741 !Series_sample_id = GSM3203742 !Series_sample_id = GSM3203743 !Series_sample_id = GSM3203744 !Series_sample_id = GSM3203745 !Series_sample_id = GSM3203746 !Series_sample_id = GSM3203747 !Series_sample_id = GSM3203748 !Series_sample_id = GSM3203749 !Series_sample_id = GSM3203750 !Series_sample_id = GSM3203751 !Series_sample_id = GSM3203752 !Series_sample_id = GSM3203753 !Series_sample_id = GSM3203754 !Series_sample_id = GSM3203755 !Series_sample_id = GSM3203756 !Series_sample_id = GSM3203757 !Series_sample_id = GSM3203758 !Series_sample_id = GSM3203759 !Series_sample_id = GSM3203760 !Series_sample_id = GSM3203761 !Series_sample_id = GSM3203762 !Series_sample_id = GSM3203763 !Series_sample_id = GSM3203764 !Series_sample_id = GSM3203765 !Series_sample_id = GSM3203766 !Series_sample_id = GSM3203767 !Series_sample_id = GSM3203768 !Series_sample_id = GSM3203769 !Series_sample_id = GSM3203770 !Series_sample_id = GSM3203771 !Series_sample_id = GSM3203772 !Series_sample_id = GSM3203773 !Series_sample_id = GSM3203774 !Series_sample_id = GSM3203775 !Series_sample_id = GSM3203776 !Series_sample_id = GSM3203777 !Series_sample_id = GSM3203778 !Series_sample_id = GSM3203779 !Series_sample_id = GSM3203780 !Series_sample_id = GSM3203781 !Series_sample_id = GSM3203782 !Series_sample_id = GSM3203783 !Series_sample_id = GSM3203784 !Series_sample_id = GSM3203785 !Series_sample_id = GSM3203786 !Series_sample_id = GSM3203787 !Series_sample_id = GSM3203788 !Series_sample_id = GSM3203789 !Series_sample_id = GSM3203790 !Series_sample_id = GSM3203791 !Series_sample_id = GSM3203792 !Series_sample_id = GSM3203793 !Series_sample_id = GSM3203794 !Series_sample_id = GSM3203795 !Series_sample_id = GSM3203796 !Series_sample_id = GSM3203797 !Series_sample_id = GSM3203798 !Series_sample_id = GSM3203799 !Series_sample_id = GSM3203800 !Series_sample_id = GSM3203801 !Series_sample_id = GSM3203802 !Series_sample_id = GSM3203803 !Series_sample_id = GSM3203804 !Series_sample_id = GSM3203805 !Series_sample_id = GSM3203806 !Series_sample_id = GSM3203807 !Series_sample_id = GSM3203808 !Series_sample_id = GSM3203809 !Series_sample_id = GSM3203810 !Series_sample_id = GSM3203811 !Series_sample_id = GSM3203812 !Series_sample_id = GSM3203813 !Series_sample_id = GSM3203814 !Series_sample_id = GSM3203815 !Series_sample_id = GSM3203816 !Series_sample_id = GSM3203817 !Series_sample_id = GSM3203818 !Series_sample_id = GSM3203819 !Series_sample_id = GSM3203820 !Series_sample_id = GSM3203821 !Series_sample_id = GSM3203822 !Series_sample_id = GSM3203823 !Series_sample_id = GSM3203824 !Series_sample_id = GSM3203825 !Series_sample_id = GSM3203826 !Series_sample_id = GSM3203827 !Series_sample_id = GSM3203828 !Series_sample_id = GSM3203829 !Series_sample_id = GSM3203830 !Series_sample_id = GSM3203831 !Series_sample_id = GSM3203832 !Series_sample_id = GSM3203833 !Series_sample_id = GSM3203834 !Series_sample_id = GSM3203835 !Series_sample_id = GSM3203836 !Series_sample_id = GSM3203837 !Series_sample_id = GSM3203838 !Series_sample_id = GSM3203839 !Series_sample_id = GSM3203840 !Series_sample_id = GSM3203841 !Series_sample_id = GSM3203842 !Series_sample_id = GSM3203843 !Series_sample_id = GSM3203844 !Series_sample_id = GSM3203845 !Series_sample_id = GSM3203846 !Series_sample_id = GSM3203847 !Series_sample_id = GSM3203848 !Series_sample_id = GSM3203849 !Series_sample_id = GSM3203850 !Series_sample_id = GSM3203851 !Series_sample_id = GSM3203852 !Series_sample_id = GSM3203853 !Series_sample_id = GSM3203854 !Series_sample_id = GSM3203855 !Series_sample_id = GSM3203856 !Series_sample_id = GSM3203857 !Series_sample_id = GSM3203858 !Series_sample_id = GSM3203859 !Series_sample_id = GSM3203860 !Series_sample_id = GSM3203861 !Series_sample_id = GSM3203862 !Series_sample_id = GSM3203863 !Series_sample_id = GSM3203864 !Series_sample_id = GSM3203865 !Series_sample_id = GSM3203866 !Series_sample_id = GSM3203867 !Series_sample_id = GSM3203868 !Series_sample_id = GSM3203869 !Series_sample_id = GSM3203870 !Series_sample_id = GSM3203871 !Series_sample_id = GSM3203872 !Series_sample_id = GSM3203873 !Series_sample_id = GSM3203874 !Series_sample_id = GSM3203875 !Series_sample_id = GSM3203876 !Series_sample_id = GSM3203877 !Series_sample_id = GSM3203878 !Series_sample_id = GSM3203879 !Series_sample_id = GSM3203880 !Series_sample_id = GSM3203881 !Series_sample_id = GSM3203882 !Series_sample_id = GSM3203883 !Series_sample_id = GSM3203884 !Series_sample_id = GSM3203885 !Series_sample_id = GSM3203886 !Series_sample_id = GSM3203887 !Series_sample_id = GSM3203888 !Series_sample_id = GSM3203889 !Series_sample_id = GSM3203890 !Series_sample_id = GSM3203891 !Series_sample_id = GSM3203892 !Series_sample_id = GSM3203893 !Series_sample_id = GSM3203894 !Series_sample_id = GSM3203895 !Series_sample_id = GSM3203896 !Series_sample_id = GSM3203897 !Series_sample_id = GSM3203898 !Series_sample_id = GSM3203899 !Series_sample_id = GSM3203900 !Series_sample_id = GSM3203901 !Series_sample_id = GSM3203902 !Series_sample_id = GSM3203903 !Series_sample_id = GSM3203904 !Series_sample_id = GSM3203905 !Series_sample_id = GSM3203906 !Series_sample_id = GSM3203907 !Series_sample_id = GSM3203908 !Series_sample_id = GSM3203909 !Series_sample_id = GSM3203910 !Series_sample_id = GSM3203911 !Series_sample_id = GSM3203912 !Series_sample_id = GSM3203913 !Series_sample_id = GSM3203914 !Series_sample_id = GSM3203915 !Series_sample_id = GSM3203916 !Series_sample_id = GSM3203917 !Series_sample_id = GSM3203918 !Series_sample_id = GSM3203919 !Series_sample_id = GSM3203920 !Series_sample_id = GSM3203921 !Series_sample_id = GSM3203922 !Series_sample_id = GSM3203923 !Series_sample_id = GSM3203924 !Series_sample_id = GSM3203925 !Series_sample_id = GSM3203926 !Series_sample_id = GSM3203927 !Series_sample_id = GSM3203928 !Series_sample_id = GSM3203929 !Series_sample_id = GSM3203930 !Series_sample_id = GSM3203931 !Series_sample_id = GSM3203932 !Series_sample_id = GSM3203933 !Series_sample_id = GSM3203934 !Series_sample_id = GSM3203935 !Series_sample_id = GSM3203936 !Series_sample_id = GSM3203937 !Series_sample_id = GSM3203938 !Series_sample_id = GSM3203939 !Series_sample_id = GSM3203940 !Series_sample_id = GSM3203941 !Series_sample_id = GSM3203942 !Series_sample_id = GSM3203943 !Series_sample_id = GSM3203944 !Series_sample_id = GSM3203945 !Series_sample_id = GSM3203946 !Series_sample_id = GSM3203947 !Series_sample_id = GSM3203948 !Series_sample_id = GSM3203949 !Series_sample_id = GSM3203950 !Series_sample_id = GSM3203951 !Series_sample_id = GSM3203952 !Series_sample_id = GSM3203953 !Series_sample_id = GSM3203954 !Series_sample_id = GSM3203955 !Series_sample_id = GSM3203956 !Series_sample_id = GSM3203957 !Series_sample_id = GSM3203958 !Series_sample_id = GSM3203959 !Series_sample_id = GSM3203960 !Series_sample_id = GSM3203961 !Series_sample_id = GSM3203962 !Series_sample_id = GSM3203963 !Series_sample_id = GSM3203964 !Series_sample_id = GSM3203965 !Series_sample_id = GSM3203966 !Series_sample_id = GSM3203967 !Series_sample_id = GSM3203968 !Series_sample_id = GSM3203969 !Series_sample_id = GSM3203970 !Series_sample_id = GSM3203971 !Series_sample_id = GSM3203972 !Series_sample_id = GSM3203973 !Series_sample_id = GSM3203974 !Series_sample_id = GSM3203975 !Series_sample_id = GSM3203976 !Series_sample_id = GSM3203977 !Series_sample_id = GSM3203978 !Series_sample_id = GSM3203979 !Series_sample_id = GSM3203980 !Series_sample_id = GSM3203981 !Series_sample_id = GSM3203982 !Series_sample_id = GSM3203983 !Series_sample_id = GSM3203984 !Series_sample_id = GSM3203985 !Series_sample_id = GSM3203986 !Series_sample_id = GSM3203987 !Series_sample_id = GSM3203988 !Series_sample_id = GSM3203989 !Series_sample_id = GSM3203990 !Series_sample_id = GSM3203991 !Series_sample_id = GSM3203992 !Series_sample_id = GSM3203993 !Series_sample_id = GSM3203994 !Series_sample_id = GSM3203995 !Series_sample_id = GSM3203996 !Series_sample_id = GSM3203997 !Series_sample_id = GSM3203998 !Series_sample_id = GSM3203999 !Series_sample_id = GSM3204000 !Series_sample_id = GSM3204001 !Series_sample_id = GSM3204002 !Series_sample_id = GSM3204003 !Series_sample_id = GSM3204004 !Series_sample_id = GSM3204005 !Series_sample_id = GSM3204006 !Series_sample_id = GSM3204007 !Series_sample_id = GSM3204008 !Series_sample_id = GSM3204009 !Series_sample_id = GSM3204010 !Series_sample_id = GSM3204011 !Series_sample_id = GSM3204012 !Series_sample_id = GSM3204013 !Series_sample_id = GSM3204014 !Series_sample_id = GSM3204015 !Series_sample_id = GSM3204016 !Series_sample_id = GSM3204017 !Series_sample_id = GSM3204018 !Series_sample_id = GSM3204019 !Series_sample_id = GSM3204020 !Series_sample_id = GSM3204021 !Series_sample_id = GSM3204022 !Series_sample_id = GSM3204023 !Series_sample_id = GSM3204024 !Series_sample_id = GSM3204025 !Series_sample_id = GSM3204026 !Series_sample_id = GSM3204027 !Series_sample_id = GSM3204028 !Series_sample_id = GSM3204029 !Series_sample_id = GSM3204030 !Series_sample_id = GSM3204031 !Series_sample_id = GSM3204032 !Series_sample_id = GSM3204033 !Series_sample_id = GSM3204034 !Series_sample_id = GSM3204035 !Series_sample_id = GSM3204036 !Series_sample_id = GSM3204037 !Series_sample_id = GSM3204038 !Series_sample_id = GSM3204039 !Series_sample_id = GSM3204040 !Series_sample_id = GSM3204041 !Series_sample_id = GSM3204042 !Series_sample_id = GSM3204043 !Series_sample_id = GSM3204044 !Series_sample_id = GSM3204045 !Series_sample_id = GSM3204046 !Series_sample_id = GSM3204047 !Series_sample_id = GSM3204048 !Series_sample_id = GSM3204049 !Series_sample_id = GSM3204050 !Series_sample_id = GSM3204051 !Series_sample_id = GSM3204052 !Series_sample_id = GSM3204053 !Series_sample_id = GSM3204054 !Series_sample_id = GSM3204055 !Series_sample_id = GSM3204056 !Series_sample_id = GSM3204057 !Series_sample_id = GSM3204058 !Series_sample_id = GSM3204059 !Series_sample_id = GSM3204060 !Series_sample_id = GSM3204061 !Series_sample_id = GSM3204062 !Series_sample_id = GSM3204063 !Series_sample_id = GSM3204064 !Series_sample_id = GSM3204065 !Series_sample_id = GSM3204066 !Series_sample_id = GSM3204067 !Series_sample_id = GSM3204068 !Series_sample_id = GSM3204069 !Series_sample_id = GSM3204070 !Series_sample_id = GSM3204071 !Series_sample_id = GSM3204072 !Series_sample_id = GSM3204073 !Series_sample_id = GSM3204074 !Series_sample_id = GSM3204075 !Series_sample_id = GSM3204076 !Series_sample_id = GSM3204077 !Series_sample_id = GSM3204078 !Series_sample_id = GSM3204079 !Series_sample_id = GSM3204080 !Series_sample_id = GSM3204081 !Series_sample_id = GSM3204082 !Series_sample_id = GSM3204083 !Series_sample_id = GSM3204084 !Series_sample_id = GSM3204085 !Series_sample_id = GSM3204086 !Series_sample_id = GSM3204087 !Series_sample_id = GSM3204088 !Series_sample_id = GSM3204089 !Series_sample_id = GSM3204090 !Series_sample_id = GSM3204091 !Series_sample_id = GSM3204092 !Series_sample_id = GSM3204093 !Series_sample_id = GSM3204094 !Series_sample_id = GSM3204095 !Series_sample_id = GSM3204096 !Series_sample_id = GSM3204097 !Series_sample_id = GSM3204098 !Series_sample_id = GSM3204099 !Series_sample_id = GSM3204100 !Series_sample_id = GSM3204101 !Series_sample_id = GSM3204102 !Series_sample_id = GSM3204103 !Series_sample_id = GSM3204104 !Series_sample_id = GSM3204105 !Series_sample_id = GSM3204106 !Series_sample_id = GSM3204107 !Series_sample_id = GSM3204108 !Series_sample_id = GSM3204109 !Series_sample_id = GSM3204110 !Series_sample_id = GSM3204111 !Series_sample_id = GSM3204112 !Series_sample_id = GSM3204113 !Series_sample_id = GSM3204114 !Series_sample_id = GSM3204115 !Series_sample_id = GSM3204116 !Series_sample_id = GSM3204117 !Series_sample_id = GSM3204118 !Series_sample_id = GSM3204119 !Series_sample_id = GSM3204120 !Series_sample_id = GSM3204121 !Series_sample_id = GSM3204122 !Series_sample_id = GSM3204123 !Series_sample_id = GSM3204124 !Series_sample_id = GSM3204125 !Series_sample_id = GSM3204126 !Series_sample_id = GSM3204127 !Series_sample_id = GSM3204128 !Series_sample_id = GSM3204129 !Series_sample_id = GSM3204130 !Series_sample_id = GSM3204131 !Series_sample_id = GSM3204132 !Series_sample_id = GSM3204133 !Series_sample_id = GSM3204134 !Series_sample_id = GSM3204135 !Series_sample_id = GSM3204136 !Series_sample_id = GSM3204137 !Series_sample_id = GSM3204138 !Series_sample_id = GSM3204139 !Series_sample_id = GSM3204140 !Series_sample_id = GSM3204141 !Series_sample_id = GSM3204142 !Series_sample_id = GSM3204143 !Series_sample_id = GSM3204144 !Series_sample_id = GSM3204145 !Series_sample_id = GSM3204146 !Series_sample_id = GSM3204147 !Series_sample_id = GSM3204148 !Series_sample_id = GSM3204149 !Series_sample_id = GSM3204150 !Series_sample_id = GSM3204151 !Series_sample_id = GSM3204152 !Series_sample_id = GSM3204153 !Series_sample_id = GSM3204154 !Series_sample_id = GSM3204155 !Series_sample_id = GSM3204156 !Series_sample_id = GSM3204157 !Series_sample_id = GSM3204158 !Series_sample_id = GSM3204159 !Series_sample_id = GSM3204160 !Series_sample_id = GSM3204161 !Series_sample_id = GSM3204162 !Series_sample_id = GSM3204163 !Series_sample_id = GSM3204164 !Series_sample_id = GSM3204165 !Series_sample_id = GSM3204166 !Series_sample_id = GSM3204167 !Series_sample_id = GSM3204168 !Series_sample_id = GSM3204169 !Series_sample_id = GSM3204170 !Series_sample_id = GSM3204171 !Series_sample_id = GSM3204172 !Series_sample_id = GSM3204173 !Series_sample_id = GSM3204174 !Series_sample_id = GSM3204175 !Series_sample_id = GSM3204176 !Series_sample_id = GSM3204177 !Series_sample_id = GSM3204178 !Series_sample_id = GSM3204179 !Series_sample_id = GSM3204180 !Series_sample_id = GSM3204181 !Series_sample_id = GSM3204182 !Series_sample_id = GSM3204183 !Series_sample_id = GSM3204184 !Series_sample_id = GSM3204185 !Series_sample_id = GSM3204186 !Series_sample_id = GSM3204187 !Series_sample_id = GSM3204188 !Series_sample_id = GSM3204189 !Series_sample_id = GSM3204190 !Series_sample_id = GSM3204191 !Series_sample_id = GSM3204192 !Series_sample_id = GSM3204193 !Series_sample_id = GSM3204194 !Series_sample_id = GSM3204195 !Series_sample_id = GSM3204196 !Series_sample_id = GSM3204197 !Series_sample_id = GSM3204198 !Series_sample_id = GSM3204199 !Series_sample_id = GSM3204200 !Series_sample_id = GSM3204201 !Series_sample_id = GSM3204202 !Series_sample_id = GSM3204203 !Series_sample_id = GSM3204204 !Series_sample_id = GSM3204205 !Series_sample_id = GSM3204206 !Series_sample_id = GSM3204207 !Series_sample_id = GSM3204208 !Series_sample_id = GSM3204209 !Series_sample_id = GSM3204210 !Series_sample_id = GSM3204211 !Series_sample_id = GSM3204212 !Series_sample_id = GSM3204213 !Series_sample_id = GSM3204214 !Series_sample_id = GSM3204215 !Series_sample_id = GSM3204216 !Series_sample_id = GSM3204217 !Series_sample_id = GSM3204218 !Series_sample_id = GSM3204219 !Series_sample_id = GSM3204220 !Series_sample_id = GSM3204221 !Series_sample_id = GSM3204222 !Series_sample_id = GSM3204223 !Series_sample_id = GSM3204224 !Series_sample_id = GSM3204225 !Series_sample_id = GSM3204226 !Series_sample_id = GSM3204227 !Series_sample_id = GSM3204228 !Series_sample_id = GSM3204229 !Series_sample_id = GSM3204230 !Series_sample_id = GSM3204231 !Series_sample_id = GSM3204232 !Series_sample_id = GSM3204233 !Series_sample_id = GSM3204234 !Series_sample_id = GSM3204235 !Series_sample_id = GSM3204236 !Series_sample_id = GSM3204237 !Series_sample_id = GSM3204238 !Series_sample_id = GSM3204239 !Series_sample_id = GSM3204240 !Series_sample_id = GSM3204241 !Series_sample_id = GSM3204242 !Series_sample_id = GSM3204243 !Series_sample_id = GSM3204244 !Series_sample_id = GSM3204245 !Series_sample_id = GSM3204246 !Series_sample_id = GSM3204247 !Series_sample_id = GSM3204248 !Series_sample_id = GSM3204249 !Series_sample_id = GSM3204250 !Series_sample_id = GSM3204251 !Series_sample_id = GSM3204252 !Series_sample_id = GSM3204253 !Series_sample_id = GSM3204254 !Series_sample_id = GSM3204255 !Series_sample_id = GSM3204256 !Series_sample_id = GSM3204257 !Series_sample_id = GSM3204258 !Series_sample_id = GSM3204259 !Series_sample_id = GSM3204260 !Series_sample_id = GSM3204261 !Series_sample_id = GSM3204262 !Series_sample_id = GSM3204263 !Series_sample_id = GSM3204264 !Series_sample_id = GSM3204265 !Series_sample_id = GSM3204266 !Series_sample_id = GSM3204267 !Series_sample_id = GSM3204268 !Series_sample_id = GSM3204269 !Series_sample_id = GSM3204270 !Series_sample_id = GSM3204271 !Series_sample_id = GSM3204272 !Series_sample_id = GSM3204273 !Series_sample_id = GSM3204274 !Series_sample_id = GSM3204275 !Series_sample_id = GSM3204276 !Series_sample_id = GSM3204277 !Series_sample_id = GSM3204278 !Series_sample_id = GSM3204279 !Series_sample_id = GSM3204280 !Series_sample_id = GSM3204281 !Series_sample_id = GSM3204282 !Series_sample_id = GSM3204283 !Series_sample_id = GSM3204284 !Series_sample_id = GSM3204285 !Series_sample_id = GSM3204286 !Series_sample_id = GSM3204287 !Series_sample_id = GSM3204288 !Series_sample_id = GSM3204289 !Series_sample_id = GSM3204290 !Series_sample_id = GSM3204291 !Series_sample_id = GSM3204292 !Series_sample_id = GSM3204293 !Series_sample_id = GSM3204294 !Series_sample_id = GSM3204295 !Series_sample_id = GSM3204296 !Series_sample_id = GSM3204297 !Series_sample_id = GSM3204298 !Series_sample_id = GSM3204299 !Series_sample_id = GSM3204300 !Series_contact_name = Orr,,Ashenberg !Series_contact_laboratory = Aviv Regev !Series_contact_department = Klarman Cell Observatory !Series_contact_institute = Broad Institute !Series_contact_address = 415 Main Street !Series_contact_city = Cambridge !Series_contact_state = MA !Series_contact_zip/postal_code = 02142 !Series_contact_country = USA !Series_supplementary_file = ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE115nnn/GSE115978/suppl/GSE115978_cell.annotations.csv.gz !Series_supplementary_file = ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE115nnn/GSE115978/suppl/GSE115978_counts.csv.gz !Series_supplementary_file = ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE115nnn/GSE115978/suppl/GSE115978_tpm.csv.gz !Series_platform_id = GPL18573 !Series_platform_taxid = 9606 !Series_sample_taxid = 9606 !Series_relation = BioProject: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA476644 ^PLATFORM = GPL18573 !Platform_title = Illumina NextSeq 500 (Homo sapiens) !Platform_geo_accession = GPL18573 !Platform_status = Public on Apr 15 2014 !Platform_submission_date = Apr 15 2014 !Platform_last_update_date = Mar 26 2019 !Platform_technology = high-throughput sequencing !Platform_distribution = virtual !Platform_organism = Homo sapiens !Platform_taxid = 9606 !Platform_contact_name = ,,GEO !Platform_contact_country = USA !Platform_data_row_count = 0 ^SAMPLE = GSM3197115 !Sample_title = cy78_CD45_neg_1_B04_S496_comb !Sample_geo_accession = GSM3197115 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445767 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197116 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_E11_S1115_comb !Sample_geo_accession = GSM3197116 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445766 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197117 !Sample_title = CY88_5_B10_S694_comb !Sample_geo_accession = GSM3197117 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445765 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197118 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_F07_S67_comb !Sample_geo_accession = GSM3197118 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445764 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197119 !Sample_title = cy78_CD45_neg_3_H06_S762_comb !Sample_geo_accession = GSM3197119 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445763 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197120 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_G01_S73_comb !Sample_geo_accession = GSM3197120 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445762 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197121 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_D09_S141_comb !Sample_geo_accession = GSM3197121 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445761 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197122 !Sample_title = CY88_3_D02_S614_comb !Sample_geo_accession = GSM3197122 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445760 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197123 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_D06_S138_comb !Sample_geo_accession = GSM3197123 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445759 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197124 !Sample_title = cy53_1_CD45_neg_C06_S318_comb !Sample_geo_accession = GSM3197124 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445758 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197125 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_C04_S28_comb !Sample_geo_accession = GSM3197125 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445757 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197126 !Sample_title = Cy71_CD45_D08_S524_comb !Sample_geo_accession = GSM3197126 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445756 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197127 !Sample_title = Cy81_FNA_CD45_B01_S301_comb !Sample_geo_accession = GSM3197127 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445755 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197128 !Sample_title = CY88_5_B06_S690_comb !Sample_geo_accession = GSM3197128 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445754 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197129 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_E04_S244_comb !Sample_geo_accession = GSM3197129 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445753 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197130 !Sample_title = Cy80_II_CD45_B07_S883_comb !Sample_geo_accession = GSM3197130 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445752 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197131 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_H11_S1151_comb !Sample_geo_accession = GSM3197131 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445751 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197132 !Sample_title = Cy81_Bulk_CD45_B10_S118_comb !Sample_geo_accession = GSM3197132 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445750 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197133 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_F10_S838_comb !Sample_geo_accession = GSM3197133 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445749 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197134 !Sample_title = CY88_3_F01_S637_comb !Sample_geo_accession = GSM3197134 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445748 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197135 !Sample_title = CY88_3_C01_S601_comb !Sample_geo_accession = GSM3197135 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445747 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197136 !Sample_title = CY89NEG_E07_S55_comb !Sample_geo_accession = GSM3197136 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445746 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197137 !Sample_title = CY89CORE11_G02_S170_comb !Sample_geo_accession = GSM3197137 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445823 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197138 !Sample_title = cy78_CD45_neg_1_D07_S523_comb !Sample_geo_accession = GSM3197138 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445822 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197139 !Sample_title = cy78_CD45_neg_3_D01_S709_comb !Sample_geo_accession = GSM3197139 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445821 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197140 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_A04_S676_comb !Sample_geo_accession = GSM3197140 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445820 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197141 !Sample_title = Cy71_CD45_B05_S497_comb !Sample_geo_accession = GSM3197141 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445819 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197142 !Sample_title = Cy80_II_CD45_C09_S897_comb !Sample_geo_accession = GSM3197142 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445818 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197143 !Sample_title = cy81_FNA_CD45_neg_D04_S1000_comb !Sample_geo_accession = GSM3197143 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445817 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197144 !Sample_title = cy82_CD45_pos_2_H12_S576_comb !Sample_geo_accession = GSM3197144 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445816 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197145 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_F04_S256_comb !Sample_geo_accession = GSM3197145 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445638 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197146 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_C01_S121_comb !Sample_geo_accession = GSM3197146 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445637 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197147 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_D02_S134_comb !Sample_geo_accession = GSM3197147 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445636 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197148 !Sample_title = cy80_CD45_neg_G01_S937_comb !Sample_geo_accession = GSM3197148 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445635 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197149 !Sample_title = cy78_CD45_neg_2_B08_S596_comb !Sample_geo_accession = GSM3197149 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445634 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197150 !Sample_title = cy78_CD45_neg_1_E02_S530_comb !Sample_geo_accession = GSM3197150 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445631 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197151 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_F07_S163_comb !Sample_geo_accession = GSM3197151 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445672 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197152 !Sample_title = CY89CORE11_A11_S107_comb !Sample_geo_accession = GSM3197152 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445671 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197153 !Sample_title = cy78_CD45_neg_3_B02_S686_comb !Sample_geo_accession = GSM3197153 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445670 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197154 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_A02_S770_comb !Sample_geo_accession = GSM3197154 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445669 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197155 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_D01_S1093_comb !Sample_geo_accession = GSM3197155 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445668 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197156 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_C01_S313_comb !Sample_geo_accession = GSM3197156 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445667 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197157 !Sample_title = cy81_Bulk_CD45_neg_A10_S106_comb !Sample_geo_accession = GSM3197157 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445666 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197158 !Sample_title = cy53_1_CD45_neg_E04_S340_comb !Sample_geo_accession = GSM3197158 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445665 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197159 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_C03_S795_comb !Sample_geo_accession = GSM3197159 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445664 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197160 !Sample_title = cy78_CD45_neg_3_D04_S712_comb !Sample_geo_accession = GSM3197160 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445663 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197161 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_A10_S10_comb !Sample_geo_accession = GSM3197161 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445662 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197162 !Sample_title = cy81_FNA_CD45_neg_B01_S973_comb !Sample_geo_accession = GSM3197162 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445661 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197163 !Sample_title = Cy81_FNA_CD45_E05_S341_comb !Sample_geo_accession = GSM3197163 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445660 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197164 !Sample_title = cy78_CD45_neg_3_E02_S722_comb !Sample_geo_accession = GSM3197164 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445659 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197165 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_H12_S288_comb !Sample_geo_accession = GSM3197165 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445658 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197166 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_D05_S713_comb !Sample_geo_accession = GSM3197166 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445657 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197167 !Sample_title = cy78_CD45_neg_3_D10_S718_comb !Sample_geo_accession = GSM3197167 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445656 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197168 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_G10_S82_comb !Sample_geo_accession = GSM3197168 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445655 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197169 !Sample_title = cy78_CD45_neg_1_G05_S557_comb !Sample_geo_accession = GSM3197169 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445654 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197170 !Sample_title = CY88_5_C12_S708_comb !Sample_geo_accession = GSM3197170 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445653 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197171 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_B02_S686_comb !Sample_geo_accession = GSM3197171 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445649 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197172 !Sample_title = Cy81_Bulk_CD45_E10_S154_comb !Sample_geo_accession = GSM3197172 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445648 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197173 !Sample_title = CY88CD45_150813_G04_S364_comb !Sample_geo_accession = GSM3197173 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445647 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197174 !Sample_title = Cy80_II_CD45_H07_S955_comb !Sample_geo_accession = GSM3197174 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445646 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197175 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_B08_S1076_comb !Sample_geo_accession = GSM3197175 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445652 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197176 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_B07_S19_comb !Sample_geo_accession = GSM3197176 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445651 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197177 !Sample_title = cy82_CD45_pos_3_A03_S3_comb !Sample_geo_accession = GSM3197177 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445650 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197178 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_C12_S1092_comb !Sample_geo_accession = GSM3197178 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445645 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197179 !Sample_title = cy78_CD45_neg_3_G11_S755_comb !Sample_geo_accession = GSM3197179 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445644 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197180 !Sample_title = Cy81_FNA_CD45_D09_S333_comb !Sample_geo_accession = GSM3197180 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445643 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197181 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_A08_S200_comb !Sample_geo_accession = GSM3197181 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445642 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197182 !Sample_title = Cy71_CD45_E12_S540_comb !Sample_geo_accession = GSM3197182 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445641 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197183 !Sample_title = cy94_cd45neg_cd90pos_G07_S367_comb !Sample_geo_accession = GSM3197183 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445640 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197184 !Sample_title = cy78_CD45_neg_1_H01_S565_comb !Sample_geo_accession = GSM3197184 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445639 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197185 !Sample_title = CY89CORE11_F05_S161_comb !Sample_geo_accession = GSM3197185 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445708 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197186 !Sample_title = CY89NEG_C03_S27_comb !Sample_geo_accession = GSM3197186 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445707 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197187 !Sample_title = cy81_Bulk_CD45_neg_G01_S169_comb !Sample_geo_accession = GSM3197187 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445706 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197188 !Sample_title = CY89CORE11_B10_S118_comb !Sample_geo_accession = GSM3197188 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445705 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197189 !Sample_title = cy78_CD45_neg_1_C02_S506_comb !Sample_geo_accession = GSM3197189 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445704 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197190 !Sample_title = CY88_5_G04_S748_comb !Sample_geo_accession = GSM3197190 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445703 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197191 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_H04_S280_comb !Sample_geo_accession = GSM3197191 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445702 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197192 !Sample_title = CY88_3_F05_S641_comb !Sample_geo_accession = GSM3197192 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445701 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197193 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_B10_S118_comb !Sample_geo_accession = GSM3197193 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445700 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197194 !Sample_title = Cy71_CD45_G02_S554_comb !Sample_geo_accession = GSM3197194 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445699 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197195 !Sample_title = cy78_CD45_neg_1_A05_S485_comb !Sample_geo_accession = GSM3197195 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445698 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197196 !Sample_title = cy81_Bulk_CD45_neg_C03_S123_comb !Sample_geo_accession = GSM3197196 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445697 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197197 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_C03_S1083_comb !Sample_geo_accession = GSM3197197 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445696 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197198 !Sample_title = cy94_cd45neg_cd90pos_E08_S344_comb !Sample_geo_accession = GSM3197198 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445695 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197199 !Sample_title = cy81_FNA_CD45_neg_C09_S993_comb !Sample_geo_accession = GSM3197199 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445694 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197200 !Sample_title = cy80_CD45_neg_G04_S940_comb !Sample_geo_accession = GSM3197200 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445693 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197201 !Sample_title = Cy80_II_CD45_H09_S957_comb !Sample_geo_accession = GSM3197201 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445692 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197202 !Sample_title = cy80_CD45_neg_A09_S873_comb !Sample_geo_accession = GSM3197202 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445691 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197203 !Sample_title = CY88_3_H11_S671_comb !Sample_geo_accession = GSM3197203 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445690 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197204 !Sample_title = Cy80_II_CD45_B11_S887_comb !Sample_geo_accession = GSM3197204 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445689 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197205 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_D12_S144_comb !Sample_geo_accession = GSM3197205 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445943 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197206 !Sample_title = CY89NEG_B03_S15_comb !Sample_geo_accession = GSM3197206 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445942 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197207 !Sample_title = Cy81_Bulk_CD45_E04_S148_comb !Sample_geo_accession = GSM3197207 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445941 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197208 !Sample_title = Cy80_II_CD45_D11_S911_comb !Sample_geo_accession = GSM3197208 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445940 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197209 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_D05_S137_comb !Sample_geo_accession = GSM3197209 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445939 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197210 !Sample_title = cy78_CD45_neg_1_E03_S531_comb !Sample_geo_accession = GSM3197210 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445938 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197211 !Sample_title = CY88_3_C10_S610_comb !Sample_geo_accession = GSM3197211 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445937 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197212 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_B08_S308_comb !Sample_geo_accession = GSM3197212 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445936 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197213 !Sample_title = cy78_CD45_neg_1_E07_S535_comb !Sample_geo_accession = GSM3197213 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445935 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197214 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_B12_S24_comb !Sample_geo_accession = GSM3197214 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446007 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197215 !Sample_title = CY89NEG_G01_S73_comb !Sample_geo_accession = GSM3197215 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446006 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197216 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_B02_S14_comb !Sample_geo_accession = GSM3197216 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446005 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197217 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_G08_S464_comb !Sample_geo_accession = GSM3197217 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446004 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197218 !Sample_title = cy81_FNA_CD45_neg_F09_S1029_comb !Sample_geo_accession = GSM3197218 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446003 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197219 !Sample_title = cy78_CD45_neg_2_C06_S606_comb !Sample_geo_accession = GSM3197219 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446002 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197220 !Sample_title = Cy81_FNA_CD45_G10_S370_comb !Sample_geo_accession = GSM3197220 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446001 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197221 !Sample_title = Cy71_CD45_E11_S539_comb !Sample_geo_accession = GSM3197221 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446000 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197222 !Sample_title = CY88_5_D11_S719_comb !Sample_geo_accession = GSM3197222 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445999 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197223 !Sample_title = cy82_CD45_pos_2_D11_S527_comb !Sample_geo_accession = GSM3197223 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445998 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197224 !Sample_title = CY89FNAQ2_F06_S450_comb !Sample_geo_accession = GSM3197224 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445997 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197225 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_C08_S32_comb !Sample_geo_accession = GSM3197225 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445996 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197226 !Sample_title = cy78_CD45_neg_3_G02_S746_comb !Sample_geo_accession = GSM3197226 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445995 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197227 !Sample_title = Cy81_FNA_CD45_C09_S321_comb !Sample_geo_accession = GSM3197227 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445994 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197228 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_F06_S66_comb !Sample_geo_accession = GSM3197228 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445993 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197229 !Sample_title = cy78_CD45_neg_1_B02_S494_comb !Sample_geo_accession = GSM3197229 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445992 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197230 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_E01_S49_comb !Sample_geo_accession = GSM3197230 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445991 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197231 !Sample_title = cy80_CD45_neg_D04_S904_comb !Sample_geo_accession = GSM3197231 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445990 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197232 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_F01_S157_comb !Sample_geo_accession = GSM3197232 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445989 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197233 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_A02_S386_comb !Sample_geo_accession = GSM3197233 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445988 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197234 !Sample_title = cy80_CD45_neg_E02_S914_comb !Sample_geo_accession = GSM3197234 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445987 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197235 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_D09_S45_comb !Sample_geo_accession = GSM3197235 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445913 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197236 !Sample_title = cy78_CD45_neg_1_H07_S571_comb !Sample_geo_accession = GSM3197236 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445912 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197237 !Sample_title = Cy80_II_CD45_G07_S943_comb !Sample_geo_accession = GSM3197237 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445911 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197238 !Sample_title = cy78_CD45_neg_1_F06_S546_comb !Sample_geo_accession = GSM3197238 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445910 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197239 !Sample_title = cy81_FNA_CD45_neg_E04_S1012_comb !Sample_geo_accession = GSM3197239 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445909 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197240 !Sample_title = cy81_Bulk_CD45_neg_E04_S148_comb !Sample_geo_accession = GSM3197240 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445908 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197241 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_H01_S181_comb !Sample_geo_accession = GSM3197241 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445907 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197242 !Sample_title = cy81_Bulk_CD45_neg_C11_S131_comb !Sample_geo_accession = GSM3197242 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445906 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197243 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_A04_S4_comb !Sample_geo_accession = GSM3197243 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445905 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197244 !Sample_title = cy81_Bulk_CD45_neg_D09_S141_comb !Sample_geo_accession = GSM3197244 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445904 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197245 !Sample_title = Cy71_CD45_G08_S560_comb !Sample_geo_accession = GSM3197245 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445903 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197246 !Sample_title = CY89CORE11_A04_S100_comb !Sample_geo_accession = GSM3197246 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445902 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197247 !Sample_title = cy80_CD45_neg_G02_S938_comb !Sample_geo_accession = GSM3197247 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445901 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197248 !Sample_title = CY88_5_H03_S759_comb !Sample_geo_accession = GSM3197248 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445900 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197249 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_B08_S20_comb !Sample_geo_accession = GSM3197249 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445899 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197250 !Sample_title = CY88_5_E04_S724_comb !Sample_geo_accession = GSM3197250 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445898 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197251 !Sample_title = CY89NEG_H11_S95_comb !Sample_geo_accession = GSM3197251 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445897 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197252 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_D11_S335_comb !Sample_geo_accession = GSM3197252 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445986 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197253 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_E02_S818_comb !Sample_geo_accession = GSM3197253 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445985 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197254 !Sample_title = cy82_CD45_pos_3_B11_S23_comb !Sample_geo_accession = GSM3197254 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445984 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197255 !Sample_title = cy81_FNA_CD45_neg_F08_S1028_comb !Sample_geo_accession = GSM3197255 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445983 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197256 !Sample_title = CY88_3_D07_S619_comb !Sample_geo_accession = GSM3197256 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445982 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197257 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_F09_S261_comb !Sample_geo_accession = GSM3197257 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445981 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197258 !Sample_title = CY88_3_G09_S657_comb !Sample_geo_accession = GSM3197258 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445980 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197259 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_D03_S807_comb !Sample_geo_accession = GSM3197259 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445979 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197260 !Sample_title = CY88_5_H08_S764_comb !Sample_geo_accession = GSM3197260 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445978 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197261 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_F08_S1124_comb !Sample_geo_accession = GSM3197261 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445977 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197262 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_D10_S334_comb !Sample_geo_accession = GSM3197262 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445976 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197263 !Sample_title = cy81_FNA_CD45_neg_G12_S1044_comb !Sample_geo_accession = GSM3197263 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445970 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197264 !Sample_title = CY88_3_B04_S592_comb !Sample_geo_accession = GSM3197264 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445969 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197265 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_A11_S683_comb !Sample_geo_accession = GSM3197265 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445975 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197266 !Sample_title = Cy71_CD45_F06_S546_comb !Sample_geo_accession = GSM3197266 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445974 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197267 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_G11_S755_comb !Sample_geo_accession = GSM3197267 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445973 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197268 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_C10_S706_comb !Sample_geo_accession = GSM3197268 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445972 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197269 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_F08_S260_comb !Sample_geo_accession = GSM3197269 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445971 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197270 !Sample_title = cy53_1_CD45_neg_B02_S302_comb !Sample_geo_accession = GSM3197270 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445968 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197271 !Sample_title = CY89NEG_F05_S65_comb !Sample_geo_accession = GSM3197271 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445967 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197272 !Sample_title = cy80_CD45_neg_H08_S956_comb !Sample_geo_accession = GSM3197272 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445966 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197273 !Sample_title = Cy81_Bulk_CD45_D01_S133_comb !Sample_geo_accession = GSM3197273 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445965 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197274 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_G03_S267_comb !Sample_geo_accession = GSM3197274 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445964 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197275 !Sample_title = Cy81_Bulk_CD45_D10_S142_comb !Sample_geo_accession = GSM3197275 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445963 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197276 !Sample_title = CY89NEG_F07_S67_comb !Sample_geo_accession = GSM3197276 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445962 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197277 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_H06_S762_comb !Sample_geo_accession = GSM3197277 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445961 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197278 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_F12_S1128_comb !Sample_geo_accession = GSM3197278 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445960 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197279 !Sample_title = cy82_CD45_pos_2_B03_S495_comb !Sample_geo_accession = GSM3197279 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445959 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197280 !Sample_title = cy78_CD45_neg_1_A04_S484_comb !Sample_geo_accession = GSM3197280 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445958 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197281 !Sample_title = Cy81_FNA_CD45_C05_S317_comb !Sample_geo_accession = GSM3197281 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445957 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197282 !Sample_title = Cy80_II_CD45_B01_S877_comb !Sample_geo_accession = GSM3197282 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445956 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197283 !Sample_title = CY89CORE11_E10_S154_comb !Sample_geo_accession = GSM3197283 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445955 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197284 !Sample_title = cy81_Bulk_CD45_neg_E02_S146_comb !Sample_geo_accession = GSM3197284 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445954 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197285 !Sample_title = cy80_CD45_neg_A05_S869_comb !Sample_geo_accession = GSM3197285 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445953 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197286 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_D03_S423_comb !Sample_geo_accession = GSM3197286 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445952 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197287 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_A04_S388_comb !Sample_geo_accession = GSM3197287 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445951 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197288 !Sample_title = CY89NEG_E06_S54_comb !Sample_geo_accession = GSM3197288 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445950 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197289 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_C02_S794_comb !Sample_geo_accession = GSM3197289 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445949 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197290 !Sample_title = CY88_3_A10_S586_comb !Sample_geo_accession = GSM3197290 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445948 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197291 !Sample_title = CY88CD45POS_7_C07_S223_comb !Sample_geo_accession = GSM3197291 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445947 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197292 !Sample_title = CY89FNAQ2_G07_S463_comb !Sample_geo_accession = GSM3197292 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445946 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197293 !Sample_title = cy80_CD45_neg_F05_S929_comb !Sample_geo_accession = GSM3197293 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445945 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197294 !Sample_title = Cy81_Bulk_CD45_G12_S180_comb !Sample_geo_accession = GSM3197294 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445944 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197295 !Sample_title = cy81_FNA_CD45_neg_F06_S1026_comb !Sample_geo_accession = GSM3197295 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445855 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197296 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_C02_S410_comb !Sample_geo_accession = GSM3197296 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445854 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197297 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_C11_S707_comb !Sample_geo_accession = GSM3197297 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445853 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197298 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_F09_S741_comb !Sample_geo_accession = GSM3197298 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445852 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197299 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_E10_S442_comb !Sample_geo_accession = GSM3197299 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445851 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197300 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_H01_S373_comb !Sample_geo_accession = GSM3197300 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445850 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197301 !Sample_title = cy78_CD45_neg_1_H08_S572_comb !Sample_geo_accession = GSM3197301 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445849 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197302 !Sample_title = CY89CORE11_B04_S112_comb !Sample_geo_accession = GSM3197302 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445848 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197303 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_F03_S159_comb !Sample_geo_accession = GSM3197303 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445847 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197304 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_H10_S286_comb !Sample_geo_accession = GSM3197304 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445846 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197305 !Sample_title = cy78_CD45_neg_1_F02_S542_comb !Sample_geo_accession = GSM3197305 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445845 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197306 !Sample_title = CY89CORE11_A05_S101_comb !Sample_geo_accession = GSM3197306 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445844 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197307 !Sample_title = CY89CORE11_B06_S114_comb !Sample_geo_accession = GSM3197307 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445843 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197308 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_E08_S56_comb !Sample_geo_accession = GSM3197308 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445842 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197309 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_C07_S223_comb !Sample_geo_accession = GSM3197309 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445841 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197310 !Sample_title = cy78_CD45_neg_3_G07_S751_comb !Sample_geo_accession = GSM3197310 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445840 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197311 !Sample_title = CY89NEG_A06_S6_comb !Sample_geo_accession = GSM3197311 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445839 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197312 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_B04_S1072_comb !Sample_geo_accession = GSM3197312 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445838 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197313 !Sample_title = Cy81_FNA_CD45_F02_S350_comb !Sample_geo_accession = GSM3197313 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445837 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197314 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_H01_S1141_comb !Sample_geo_accession = GSM3197314 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445836 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197315 !Sample_title = CY89CORE11_D09_S141_comb !Sample_geo_accession = GSM3197315 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445835 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197316 !Sample_title = cy78_CD45_neg_1_F10_S550_comb !Sample_geo_accession = GSM3197316 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445834 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197317 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_H11_S479_comb !Sample_geo_accession = GSM3197317 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445833 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197318 !Sample_title = cy81_FNA_CD45_neg_H09_S1053_comb !Sample_geo_accession = GSM3197318 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445832 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197319 !Sample_title = cy78_CD45_neg_3_E05_S725_comb !Sample_geo_accession = GSM3197319 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445831 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197320 !Sample_title = cy78_CD45_neg_1_H04_S568_comb !Sample_geo_accession = GSM3197320 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445830 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197321 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_A07_S7_comb !Sample_geo_accession = GSM3197321 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445829 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197322 !Sample_title = CY89FNAQ2_B02_S398_comb !Sample_geo_accession = GSM3197322 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445828 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197323 !Sample_title = Cy71_CD45_H06_S570_comb !Sample_geo_accession = GSM3197323 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445812 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197324 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_D09_S237_comb !Sample_geo_accession = GSM3197324 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445811 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197325 !Sample_title = CY88_5_B12_S696_comb !Sample_geo_accession = GSM3197325 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445827 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197326 !Sample_title = CY88_3_H10_S670_comb !Sample_geo_accession = GSM3197326 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445826 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197327 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_B11_S119_comb !Sample_geo_accession = GSM3197327 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445815 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197328 !Sample_title = CY89FNAQ2_C04_S412_comb !Sample_geo_accession = GSM3197328 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445814 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197329 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_E10_S730_comb !Sample_geo_accession = GSM3197329 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445813 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197330 !Sample_title = Cy80_II_CD45_B09_S885_comb !Sample_geo_accession = GSM3197330 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445810 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197331 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_G02_S746_comb !Sample_geo_accession = GSM3197331 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445809 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197332 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_G02_S170_comb !Sample_geo_accession = GSM3197332 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445808 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197333 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_E05_S245_comb !Sample_geo_accession = GSM3197333 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445807 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197334 !Sample_title = CY88_5_H05_S761_comb !Sample_geo_accession = GSM3197334 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445806 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197335 !Sample_title = Cy81_FNA_CD45_H12_S384_comb !Sample_geo_accession = GSM3197335 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445805 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197336 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_G04_S844_comb !Sample_geo_accession = GSM3197336 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445804 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197337 !Sample_title = CY88_5_D08_S716_comb !Sample_geo_accession = GSM3197337 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445803 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197338 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_B11_S407_comb !Sample_geo_accession = GSM3197338 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445802 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197339 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_B11_S215_comb !Sample_geo_accession = GSM3197339 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445801 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197340 !Sample_title = CY88_3_B12_S600_comb !Sample_geo_accession = GSM3197340 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445800 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197341 !Sample_title = cy81_FNA_CD45_neg_F02_S1022_comb !Sample_geo_accession = GSM3197341 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445799 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197342 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_D05_S425_comb !Sample_geo_accession = GSM3197342 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445798 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197343 !Sample_title = cy94_cd45neg_cd90pos_A11_S299_comb !Sample_geo_accession = GSM3197343 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445797 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197344 !Sample_title = cy60_1_cd_45_neg_3_F06_S546_comb !Sample_geo_accession = GSM3197344 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445796 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197345 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_D01_S421_comb !Sample_geo_accession = GSM3197345 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445795 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197346 !Sample_title = CY88_5_D10_S718_comb !Sample_geo_accession = GSM3197346 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445794 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197347 !Sample_title = CY89NEG_H06_S90_comb !Sample_geo_accession = GSM3197347 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445793 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197348 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_H03_S375_comb !Sample_geo_accession = GSM3197348 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445792 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197349 !Sample_title = Cy81_Bulk_CD45_E08_S152_comb !Sample_geo_accession = GSM3197349 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445791 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197350 !Sample_title = CY89FNAQ2_H03_S471_comb !Sample_geo_accession = GSM3197350 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445790 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197351 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_H09_S1149_comb !Sample_geo_accession = GSM3197351 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445859 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197352 !Sample_title = cy81_FNA_CD45_neg_B04_S976_comb !Sample_geo_accession = GSM3197352 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445858 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197353 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_F11_S167_comb !Sample_geo_accession = GSM3197353 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445857 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197354 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_F03_S1119_comb !Sample_geo_accession = GSM3197354 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445856 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197355 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_C04_S412_comb !Sample_geo_accession = GSM3197355 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445825 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197356 !Sample_title = Cy80_II_CD45_G09_S945_comb !Sample_geo_accession = GSM3197356 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445824 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197357 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_G05_S749_comb !Sample_geo_accession = GSM3197357 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445896 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197358 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_F06_S450_comb !Sample_geo_accession = GSM3197358 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445895 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197359 !Sample_title = cy82_CD45_pos_3_C11_S35_comb !Sample_geo_accession = GSM3197359 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445894 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197360 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_C04_S316_comb !Sample_geo_accession = GSM3197360 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445893 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197361 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_F06_S162_comb !Sample_geo_accession = GSM3197361 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445892 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197362 !Sample_title = CY88_3_C12_S612_comb !Sample_geo_accession = GSM3197362 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445891 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197363 !Sample_title = Cy80_II_CD45_D09_S909_comb !Sample_geo_accession = GSM3197363 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445890 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197364 !Sample_title = cy81_FNA_CD45_neg_E12_S1020_comb !Sample_geo_accession = GSM3197364 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445889 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197365 !Sample_title = cy78_CD45_neg_3_G09_S753_comb !Sample_geo_accession = GSM3197365 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445888 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197366 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_A12_S1068_comb !Sample_geo_accession = GSM3197366 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445887 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197367 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_B11_S1079_comb !Sample_geo_accession = GSM3197367 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445886 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197368 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_A05_S773_comb !Sample_geo_accession = GSM3197368 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445885 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197369 !Sample_title = Cy71_CD45_D11_S527_comb !Sample_geo_accession = GSM3197369 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445884 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197370 !Sample_title = cy80_CD45_neg_D03_S903_comb !Sample_geo_accession = GSM3197370 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445883 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197371 !Sample_title = cy78_CD45_neg_1_B08_S500_comb !Sample_geo_accession = GSM3197371 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445882 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197372 !Sample_title = cy88_cd45pos_5_C01_S217_comb !Sample_geo_accession = GSM3197372 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445881 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197373 !Sample_title = cy81_Bulk_CD45_neg_E09_S153_comb !Sample_geo_accession = GSM3197373 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445880 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197374 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_B06_S210_comb !Sample_geo_accession = GSM3197374 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445879 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197375 !Sample_title = cy78_CD45_neg_3_D07_S715_comb !Sample_geo_accession = GSM3197375 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445878 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197376 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_F05_S833_comb !Sample_geo_accession = GSM3197376 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445877 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197377 !Sample_title = cy80_CD45_neg_B01_S877_comb !Sample_geo_accession = GSM3197377 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445876 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197378 !Sample_title = cy82_CD45_pos_1_E08_S536_comb !Sample_geo_accession = GSM3197378 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445875 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197379 !Sample_title = Cy81_Bulk_CD45_B09_S117_comb !Sample_geo_accession = GSM3197379 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445874 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197380 !Sample_title = cy78_CD45_neg_1_A03_S483_comb !Sample_geo_accession = GSM3197380 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445873 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197381 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_H06_S858_comb !Sample_geo_accession = GSM3197381 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445872 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197382 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_A03_S1059_comb !Sample_geo_accession = GSM3197382 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445871 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197383 !Sample_title = cy78_CD45_neg_1_F08_S548_comb !Sample_geo_accession = GSM3197383 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445865 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197384 !Sample_title = cy60_1_cd_45_neg_3_C10_S514_comb !Sample_geo_accession = GSM3197384 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445864 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197385 !Sample_title = CY88_3_C08_S608_comb !Sample_geo_accession = GSM3197385 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445870 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197386 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_A12_S108_comb !Sample_geo_accession = GSM3197386 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445869 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197387 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_H10_S862_comb !Sample_geo_accession = GSM3197387 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445868 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197388 !Sample_title = Cy71_CD45_G09_S561_comb !Sample_geo_accession = GSM3197388 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445867 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197389 !Sample_title = cy80_CD45_neg_C09_S897_comb !Sample_geo_accession = GSM3197389 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445866 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197390 !Sample_title = Cy71_CD45_F11_S551_comb !Sample_geo_accession = GSM3197390 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445863 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197391 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_B04_S304_comb !Sample_geo_accession = GSM3197391 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445862 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197392 !Sample_title = cy78_CD45_neg_3_D08_S716_comb !Sample_geo_accession = GSM3197392 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445861 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197393 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_E03_S723_comb !Sample_geo_accession = GSM3197393 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445860 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197394 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_F04_S160_comb !Sample_geo_accession = GSM3197394 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445934 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197395 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_C12_S420_comb !Sample_geo_accession = GSM3197395 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445933 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197396 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_C08_S128_comb !Sample_geo_accession = GSM3197396 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445932 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197397 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_E08_S728_comb !Sample_geo_accession = GSM3197397 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445931 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197398 !Sample_title = cy80_CD45_neg_D07_S907_comb !Sample_geo_accession = GSM3197398 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445930 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197399 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_F03_S63_comb !Sample_geo_accession = GSM3197399 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445929 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197400 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_H07_S859_comb !Sample_geo_accession = GSM3197400 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445928 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197401 !Sample_title = cy79_p5_CD45_pos_PD1_pos_E02_S626_comb !Sample_geo_accession = GSM3197401 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445927 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197402 !Sample_title = cy80_CD45_neg_A03_S867_comb !Sample_geo_accession = GSM3197402 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445926 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197403 !Sample_title = Cy81_FNA_CD45_B10_S310_comb !Sample_geo_accession = GSM3197403 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445925 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197404 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_H07_S187_comb !Sample_geo_accession = GSM3197404 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445924 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197405 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_B07_S115_comb !Sample_geo_accession = GSM3197405 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445923 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197406 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_G09_S849_comb !Sample_geo_accession = GSM3197406 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445922 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197407 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_C09_S801_comb !Sample_geo_accession = GSM3197407 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445921 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197408 !Sample_title = CY88_5_E02_S722_comb !Sample_geo_accession = GSM3197408 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445920 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197409 !Sample_title = cy80_CD45_neg_H09_S957_comb !Sample_geo_accession = GSM3197409 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445919 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197410 !Sample_title = cy53_1_CD45_neg_E12_S348_comb !Sample_geo_accession = GSM3197410 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445918 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197411 !Sample_title = cy80_CD45_neg_H02_S950_comb !Sample_geo_accession = GSM3197411 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445917 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197412 !Sample_title = cy78_CD45_neg_2_C05_S605_comb !Sample_geo_accession = GSM3197412 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445916 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197413 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_G04_S172_comb !Sample_geo_accession = GSM3197413 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445915 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197414 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_G03_S843_comb !Sample_geo_accession = GSM3197414 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445914 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197415 !Sample_title = cy78_CD45_neg_1_G04_S556_comb !Sample_geo_accession = GSM3197415 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445731 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197416 !Sample_title = cy53_1_CD45_neg_H12_S384_comb !Sample_geo_accession = GSM3197416 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445730 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197417 !Sample_title = cy78_CD45_neg_1_D05_S521_comb !Sample_geo_accession = GSM3197417 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445729 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197418 !Sample_title = Cy71_CD45_H10_S574_comb !Sample_geo_accession = GSM3197418 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445728 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197419 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_D12_S432_comb !Sample_geo_accession = GSM3197419 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445727 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197420 !Sample_title = cy81_Bulk_CD45_neg_C08_S128_comb !Sample_geo_accession = GSM3197420 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445726 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197421 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_G07_S79_comb !Sample_geo_accession = GSM3197421 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445725 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197422 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_A08_S392_comb !Sample_geo_accession = GSM3197422 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445724 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197423 !Sample_title = cy78_CD45_neg_1_C10_S514_comb !Sample_geo_accession = GSM3197423 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445723 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197424 !Sample_title = cy78_CD45_neg_3_D09_S717_comb !Sample_geo_accession = GSM3197424 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445722 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197425 !Sample_title = Cy81_Bulk_CD45_C06_S126_comb !Sample_geo_accession = GSM3197425 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445721 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197426 !Sample_title = cy82_CD45_pos_2_E07_S535_comb !Sample_geo_accession = GSM3197426 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445720 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197427 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_G12_S180_comb !Sample_geo_accession = GSM3197427 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445719 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197428 !Sample_title = Cy81_FNA_CD45_H10_S382_comb !Sample_geo_accession = GSM3197428 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445718 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197429 !Sample_title = Cy80_II_CD45_B02_S878_comb !Sample_geo_accession = GSM3197429 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445717 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197430 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_B10_S310_comb !Sample_geo_accession = GSM3197430 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445716 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197431 !Sample_title = cy80_CD45_neg_E01_S913_comb !Sample_geo_accession = GSM3197431 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445715 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197432 !Sample_title = Cy81_FNA_CD45_B11_S311_comb !Sample_geo_accession = GSM3197432 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445714 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197433 !Sample_title = cy81_Bulk_CD45_neg_C04_S124_comb !Sample_geo_accession = GSM3197433 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445713 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197434 !Sample_title = CY88_3_G07_S655_comb !Sample_geo_accession = GSM3197434 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445712 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197435 !Sample_title = Cy81_Bulk_CD45_B12_S120_comb !Sample_geo_accession = GSM3197435 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445711 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197436 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_G03_S75_comb !Sample_geo_accession = GSM3197436 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445710 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197437 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_F11_S455_comb !Sample_geo_accession = GSM3197437 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445709 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197438 !Sample_title = cy78_CD45_neg_3_B08_S692_comb !Sample_geo_accession = GSM3197438 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445789 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197439 !Sample_title = cy78_CD45_neg_3_C09_S705_comb !Sample_geo_accession = GSM3197439 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445788 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197440 !Sample_title = cy82_CD45_pos_1_H05_S569_comb !Sample_geo_accession = GSM3197440 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445787 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197441 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_G09_S81_comb !Sample_geo_accession = GSM3197441 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445786 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197442 !Sample_title = CY88_5_F11_S743_comb !Sample_geo_accession = GSM3197442 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445687 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197443 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_B04_S112_comb !Sample_geo_accession = GSM3197443 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445686 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197444 !Sample_title = cy80_CD45_neg_H03_S951_comb !Sample_geo_accession = GSM3197444 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445685 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197445 !Sample_title = cy78_CD45_neg_1_H02_S566_comb !Sample_geo_accession = GSM3197445 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445785 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197446 !Sample_title = CY88_5_C09_S705_comb !Sample_geo_accession = GSM3197446 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445784 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197447 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_F07_S259_comb !Sample_geo_accession = GSM3197447 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445783 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197448 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_C10_S34_comb !Sample_geo_accession = GSM3197448 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445688 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197449 !Sample_title = cy81_Bulk_CD45_neg_B03_S111_comb !Sample_geo_accession = GSM3197449 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445684 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197450 !Sample_title = cy81_Bulk_CD45_neg_E08_S152_comb !Sample_geo_accession = GSM3197450 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445683 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197451 !Sample_title = cy84_Primary_CD45_neg_G07_S1039_comb !Sample_geo_accession = GSM3197451 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445682 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197452 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_D06_S1098_comb !Sample_geo_accession = GSM3197452 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445681 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197453 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_B09_S1077_comb !Sample_geo_accession = GSM3197453 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445680 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197454 !Sample_title = cy53_1_CD45_neg_D06_S330_comb !Sample_geo_accession = GSM3197454 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445679 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197455 !Sample_title = Cy71_CD45_A10_S490_comb !Sample_geo_accession = GSM3197455 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445678 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197456 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_B10_S406_comb !Sample_geo_accession = GSM3197456 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445677 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197457 !Sample_title = Cy80_II_CD45_F12_S936_comb !Sample_geo_accession = GSM3197457 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445676 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197458 !Sample_title = cy80_CD45_neg_D10_S910_comb !Sample_geo_accession = GSM3197458 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445675 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197459 !Sample_title = Cy81_Bulk_CD45_B01_S109_comb !Sample_geo_accession = GSM3197459 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445674 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197460 !Sample_title = Cy80_II_CD45_B06_S882_comb !Sample_geo_accession = GSM3197460 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445673 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197461 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_F02_S734_comb !Sample_geo_accession = GSM3197461 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445745 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197462 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_G07_S847_comb !Sample_geo_accession = GSM3197462 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445744 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197463 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_E11_S59_comb !Sample_geo_accession = GSM3197463 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445743 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197464 !Sample_title = Cy71_CD45_F10_S550_comb !Sample_geo_accession = GSM3197464 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445742 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197465 !Sample_title = cy84_Primary_CD45_neg_A03_S963_comb !Sample_geo_accession = GSM3197465 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445741 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197466 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_C07_S127_comb !Sample_geo_accession = GSM3197466 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445740 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197467 !Sample_title = Cy71_CD45_D09_S525_comb !Sample_geo_accession = GSM3197467 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445739 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197468 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_H03_S1143_comb !Sample_geo_accession = GSM3197468 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445738 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197469 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_H10_S1150_comb !Sample_geo_accession = GSM3197469 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445737 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197470 !Sample_title = CY89CORE11_F03_S159_comb !Sample_geo_accession = GSM3197470 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445736 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197471 !Sample_title = CY89FNAQ2_B10_S406_comb !Sample_geo_accession = GSM3197471 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445735 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197472 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_D02_S422_comb !Sample_geo_accession = GSM3197472 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445734 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197473 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_D10_S46_comb !Sample_geo_accession = GSM3197473 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445733 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197474 !Sample_title = Cy80_II_CD45_F07_S931_comb !Sample_geo_accession = GSM3197474 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445732 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197475 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_A11_S395_comb !Sample_geo_accession = GSM3197475 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445782 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197476 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_G06_S78_comb !Sample_geo_accession = GSM3197476 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445781 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197477 !Sample_title = CY88_5_B03_S687_comb !Sample_geo_accession = GSM3197477 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445780 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197478 !Sample_title = Cy71_CD45_E10_S538_comb !Sample_geo_accession = GSM3197478 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445779 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197479 !Sample_title = cy84_Primary_CD45_neg_E12_S1020_comb !Sample_geo_accession = GSM3197479 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445778 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197480 !Sample_title = CY89NEG_A03_S3_comb !Sample_geo_accession = GSM3197480 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445777 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197481 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_G07_S1135_comb !Sample_geo_accession = GSM3197481 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445776 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197482 !Sample_title = cy78_CD45_neg_1_F05_S545_comb !Sample_geo_accession = GSM3197482 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445775 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197483 !Sample_title = CY88_5_B07_S691_comb !Sample_geo_accession = GSM3197483 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445774 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197484 !Sample_title = cy82_CD45_pos_2_F11_S551_comb !Sample_geo_accession = GSM3197484 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445773 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197485 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_C10_S418_comb !Sample_geo_accession = GSM3197485 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445772 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197486 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_E02_S434_comb !Sample_geo_accession = GSM3197486 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445771 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197487 !Sample_title = CY88_5_B01_S685_comb !Sample_geo_accession = GSM3197487 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445770 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197488 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_H02_S374_comb !Sample_geo_accession = GSM3197488 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445769 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197489 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_F09_S453_comb !Sample_geo_accession = GSM3197489 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09445768 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197490 !Sample_title = CY89FNAQ2_C01_S409_comb !Sample_geo_accession = GSM3197490 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446299 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197491 !Sample_title = Cy80_II_CD45_B12_S888_comb !Sample_geo_accession = GSM3197491 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446298 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197492 !Sample_title = cy80_CD45_neg_D09_S909_comb !Sample_geo_accession = GSM3197492 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446297 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197493 !Sample_title = CY89CORE11_G08_S176_comb !Sample_geo_accession = GSM3197493 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446296 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197494 !Sample_title = CY88_3_E10_S634_comb !Sample_geo_accession = GSM3197494 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446295 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197495 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_C11_S1091_comb !Sample_geo_accession = GSM3197495 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446294 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197496 !Sample_title = Cy80_II_CD45_A09_S873_comb !Sample_geo_accession = GSM3197496 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446293 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197497 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_B04_S688_comb !Sample_geo_accession = GSM3197497 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446292 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197498 !Sample_title = cy81_Bulk_CD45_neg_F05_S161_comb !Sample_geo_accession = GSM3197498 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446291 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197499 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_E06_S726_comb !Sample_geo_accession = GSM3197499 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446290 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197500 !Sample_title = cy81_FNA_CD45_neg_G03_S1035_comb !Sample_geo_accession = GSM3197500 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446289 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197501 !Sample_title = cy82_CD45_neg_1_D05_S233_comb !Sample_geo_accession = GSM3197501 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446288 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197502 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_F11_S839_comb !Sample_geo_accession = GSM3197502 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446287 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197503 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_F02_S830_comb !Sample_geo_accession = GSM3197503 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446286 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197504 !Sample_title = cy81_FNA_CD45_neg_G09_S1041_comb !Sample_geo_accession = GSM3197504 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446328 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197505 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_B09_S117_comb !Sample_geo_accession = GSM3197505 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446327 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197506 !Sample_title = CY89NEG_A02_S2_comb !Sample_geo_accession = GSM3197506 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446326 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197507 !Sample_title = cy78_CD45_neg_3_F12_S744_comb !Sample_geo_accession = GSM3197507 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446325 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197508 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_G03_S459_comb !Sample_geo_accession = GSM3197508 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446324 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197509 !Sample_title = Cy80_II_CD45_F11_S935_comb !Sample_geo_accession = GSM3197509 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446323 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197510 !Sample_title = CY89NEG_E02_S50_comb !Sample_geo_accession = GSM3197510 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446322 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197511 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_A11_S203_comb !Sample_geo_accession = GSM3197511 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446321 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197512 !Sample_title = CY89CORE11_F07_S163_comb !Sample_geo_accession = GSM3197512 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446320 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197513 !Sample_title = cy78_CD45_neg_3_A05_S677_comb !Sample_geo_accession = GSM3197513 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446319 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197514 !Sample_title = CY89CORE11_A09_S105_comb !Sample_geo_accession = GSM3197514 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446318 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197515 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_H09_S189_comb !Sample_geo_accession = GSM3197515 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446317 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197516 !Sample_title = cy78_CD45_neg_1_D09_S525_comb !Sample_geo_accession = GSM3197516 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446316 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197517 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_A12_S780_comb !Sample_geo_accession = GSM3197517 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446315 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197518 !Sample_title = cy78_CD45_neg_1_D12_S528_comb !Sample_geo_accession = GSM3197518 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446314 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197519 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_A08_S1064_comb !Sample_geo_accession = GSM3197519 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446313 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197520 !Sample_title = cy80_CD45_neg_D11_S911_comb !Sample_geo_accession = GSM3197520 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446312 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197521 !Sample_title = Cy81_Bulk_CD45_E03_S147_comb !Sample_geo_accession = GSM3197521 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446311 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197522 !Sample_title = CY89NEG_D12_S48_comb !Sample_geo_accession = GSM3197522 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446310 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197523 !Sample_title = cy82_CD45_neg_2_H12_S288_comb !Sample_geo_accession = GSM3197523 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446309 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197524 !Sample_title = CY89A_CD45_POS_10_A07_S199_comb !Sample_geo_accession = GSM3197524 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446308 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197525 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_E01_S1105_comb !Sample_geo_accession = GSM3197525 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446383 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197526 !Sample_title = cy82_CD45_neg_3_D09_S813_comb !Sample_geo_accession = GSM3197526 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446382 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197527 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_B03_S1071_comb !Sample_geo_accession = GSM3197527 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446381 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197528 !Sample_title = CY88_5_C08_S704_comb !Sample_geo_accession = GSM3197528 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446380 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197529 !Sample_title = cy78_CD45_neg_3_C01_S697_comb !Sample_geo_accession = GSM3197529 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446379 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197530 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_A11_S779_comb !Sample_geo_accession = GSM3197530 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446378 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197531 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_B06_S306_comb !Sample_geo_accession = GSM3197531 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446376 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197532 !Sample_title = CY89CORE11_C08_S128_comb !Sample_geo_accession = GSM3197532 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446375 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197533 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_D04_S1096_comb !Sample_geo_accession = GSM3197533 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446374 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197534 !Sample_title = cy80_CD45_neg_E06_S918_comb !Sample_geo_accession = GSM3197534 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446043 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197535 !Sample_title = Cy80_II_CD45_D04_S904_comb !Sample_geo_accession = GSM3197535 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446042 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197536 !Sample_title = CY89CORE11_D11_S143_comb !Sample_geo_accession = GSM3197536 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446041 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197537 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_H03_S759_comb !Sample_geo_accession = GSM3197537 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446040 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197538 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_C05_S317_comb !Sample_geo_accession = GSM3197538 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446039 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197539 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_A07_S775_comb !Sample_geo_accession = GSM3197539 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446038 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197540 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_C05_S413_comb !Sample_geo_accession = GSM3197540 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446037 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197541 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_D08_S428_comb !Sample_geo_accession = GSM3197541 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446036 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197542 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_D07_S331_comb !Sample_geo_accession = GSM3197542 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446035 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197543 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_H11_S767_comb !Sample_geo_accession = GSM3197543 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446034 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197544 !Sample_title = cy80_CD45_neg_B04_S880_comb !Sample_geo_accession = GSM3197544 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446033 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197545 !Sample_title = Cy71_CD45_B12_S504_comb !Sample_geo_accession = GSM3197545 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446032 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197546 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_A10_S106_comb !Sample_geo_accession = GSM3197546 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446031 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197547 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_B10_S1078_comb !Sample_geo_accession = GSM3197547 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446030 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197548 !Sample_title = cy78_CD45_neg_3_A12_S684_comb !Sample_geo_accession = GSM3197548 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446029 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197549 !Sample_title = CY88_5_F05_S737_comb !Sample_geo_accession = GSM3197549 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446028 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197550 !Sample_title = CY88_5_C05_S701_comb !Sample_geo_accession = GSM3197550 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446027 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197551 !Sample_title = cy53_1_CD45_neg_B07_S307_comb !Sample_geo_accession = GSM3197551 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446026 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197552 !Sample_title = CY88_3_G11_S659_comb !Sample_geo_accession = GSM3197552 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446025 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197553 !Sample_title = Cy81_Bulk_CD45_A11_S107_comb !Sample_geo_accession = GSM3197553 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446024 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197554 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_B03_S207_comb !Sample_geo_accession = GSM3197554 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446023 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197555 !Sample_title = CY89CORE11_H06_S186_comb !Sample_geo_accession = GSM3197555 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446022 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197556 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_G08_S80_comb !Sample_geo_accession = GSM3197556 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446021 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197557 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_E04_S52_comb !Sample_geo_accession = GSM3197557 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446020 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197558 !Sample_title = CY89FNAQ2_F12_S456_comb !Sample_geo_accession = GSM3197558 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446019 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197559 !Sample_title = cy81_FNA_CD45_neg_F12_S1032_comb !Sample_geo_accession = GSM3197559 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446018 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197560 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_F02_S1118_comb !Sample_geo_accession = GSM3197560 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446017 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197561 !Sample_title = cy78_CD45_neg_2_A12_S588_comb !Sample_geo_accession = GSM3197561 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446016 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197562 !Sample_title = Cy71_CD45_E02_S530_comb !Sample_geo_accession = GSM3197562 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446015 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197563 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_E11_S347_comb !Sample_geo_accession = GSM3197563 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446014 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197564 !Sample_title = Cy71_CD45_B03_S495_comb !Sample_geo_accession = GSM3197564 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446013 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197565 !Sample_title = Cy81_FNA_CD45_C08_S320_comb !Sample_geo_accession = GSM3197565 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446012 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197566 !Sample_title = CY88_3_D03_S615_comb !Sample_geo_accession = GSM3197566 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446011 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197567 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_G03_S747_comb !Sample_geo_accession = GSM3197567 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446010 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197568 !Sample_title = CY89NEG_F04_S64_comb !Sample_geo_accession = GSM3197568 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446009 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197569 !Sample_title = Cy81_Bulk_CD45_C05_S125_comb !Sample_geo_accession = GSM3197569 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446008 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197570 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_A12_S396_comb !Sample_geo_accession = GSM3197570 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446078 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197571 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_H10_S766_comb !Sample_geo_accession = GSM3197571 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446077 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197572 !Sample_title = cy80_CD45_neg_D01_S901_comb !Sample_geo_accession = GSM3197572 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446076 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197573 !Sample_title = CY89NEG_D05_S41_comb !Sample_geo_accession = GSM3197573 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446075 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197574 !Sample_title = cy80_CD45_neg_C02_S890_comb !Sample_geo_accession = GSM3197574 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446074 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197575 !Sample_title = CY88_3_D09_S621_comb !Sample_geo_accession = GSM3197575 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446073 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197576 !Sample_title = cy78_CD45_neg_1_D01_S517_comb !Sample_geo_accession = GSM3197576 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446072 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197577 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_B04_S16_comb !Sample_geo_accession = GSM3197577 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446071 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197578 !Sample_title = cy78_CD45_neg_3_B01_S685_comb !Sample_geo_accession = GSM3197578 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446070 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197579 !Sample_title = cy80_CD45_neg_E10_S922_comb !Sample_geo_accession = GSM3197579 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446069 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197580 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_H04_S1144_comb !Sample_geo_accession = GSM3197580 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446068 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197581 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_C02_S1082_comb !Sample_geo_accession = GSM3197581 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446067 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197582 !Sample_title = cy78_CD45_neg_1_B11_S503_comb !Sample_geo_accession = GSM3197582 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446066 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197583 !Sample_title = CY88_3_A07_S583_comb !Sample_geo_accession = GSM3197583 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446065 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197584 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_C11_S419_comb !Sample_geo_accession = GSM3197584 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446064 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197585 !Sample_title = CY88_5_C02_S698_comb !Sample_geo_accession = GSM3197585 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446063 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197586 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_G03_S1131_comb !Sample_geo_accession = GSM3197586 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446062 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197587 !Sample_title = cy78_CD45_neg_2_B10_S598_comb !Sample_geo_accession = GSM3197587 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446061 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197588 !Sample_title = Cy71_CD45_F01_S541_comb !Sample_geo_accession = GSM3197588 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446060 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197589 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_F07_S1123_comb !Sample_geo_accession = GSM3197589 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446058 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197590 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_C09_S1089_comb !Sample_geo_accession = GSM3197590 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446057 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197591 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_D10_S814_comb !Sample_geo_accession = GSM3197591 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446056 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197592 !Sample_title = CY89NEG_F08_S68_comb !Sample_geo_accession = GSM3197592 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446055 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197593 !Sample_title = CY89CORE11_D05_S137_comb !Sample_geo_accession = GSM3197593 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446251 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197594 !Sample_title = cy78_CD45_neg_1_B06_S498_comb !Sample_geo_accession = GSM3197594 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446250 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197595 !Sample_title = CY88_3_A02_S578_comb !Sample_geo_accession = GSM3197595 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446059 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197596 !Sample_title = cy81_Bulk_CD45_neg_G04_S172_comb !Sample_geo_accession = GSM3197596 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446249 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197597 !Sample_title = Cy81_Bulk_CD45_B04_S112_comb !Sample_geo_accession = GSM3197597 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446248 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197598 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_F08_S836_comb !Sample_geo_accession = GSM3197598 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446247 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197599 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_E01_S241_comb !Sample_geo_accession = GSM3197599 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446246 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197600 !Sample_title = CY89CORE11_A03_S99_comb !Sample_geo_accession = GSM3197600 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446245 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197601 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_D08_S332_comb !Sample_geo_accession = GSM3197601 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446244 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197602 !Sample_title = cy78_CD45_neg_1_H03_S567_comb !Sample_geo_accession = GSM3197602 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446243 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197603 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_D11_S1103_comb !Sample_geo_accession = GSM3197603 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446242 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197604 !Sample_title = Cy71_CD45_G05_S557_comb !Sample_geo_accession = GSM3197604 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446241 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197605 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_F03_S351_comb !Sample_geo_accession = GSM3197605 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446240 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197606 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_C10_S226_comb !Sample_geo_accession = GSM3197606 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446239 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197607 !Sample_title = CY89NEG_A07_S7_comb !Sample_geo_accession = GSM3197607 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446238 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197608 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_F09_S1125_comb !Sample_geo_accession = GSM3197608 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446237 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197609 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_C05_S125_comb !Sample_geo_accession = GSM3197609 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446236 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197610 !Sample_title = cy78_CD45_neg_3_E07_S727_comb !Sample_geo_accession = GSM3197610 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446235 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197611 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_G07_S751_comb !Sample_geo_accession = GSM3197611 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446234 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197612 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_C09_S225_comb !Sample_geo_accession = GSM3197612 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446233 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197613 !Sample_title = cy78_CD45_neg_1_G12_S564_comb !Sample_geo_accession = GSM3197613 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446232 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197614 !Sample_title = cy84_Primary_CD45_neg_E05_S1013_comb !Sample_geo_accession = GSM3197614 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446231 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197615 !Sample_title = cy81_Bulk_CD45_neg_G07_S175_comb !Sample_geo_accession = GSM3197615 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446230 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197616 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_B09_S213_comb !Sample_geo_accession = GSM3197616 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446307 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197617 !Sample_title = cy78_CD45_neg_3_H02_S758_comb !Sample_geo_accession = GSM3197617 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446306 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197618 !Sample_title = cy78_CD45_neg_3_F09_S741_comb !Sample_geo_accession = GSM3197618 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446305 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197619 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_H02_S278_comb !Sample_geo_accession = GSM3197619 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446304 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197620 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_G04_S748_comb !Sample_geo_accession = GSM3197620 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446303 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197621 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_B06_S402_comb !Sample_geo_accession = GSM3197621 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446302 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197622 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_C02_S218_comb !Sample_geo_accession = GSM3197622 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446301 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197623 !Sample_title = Cy81_FNA_CD45_F03_S351_comb !Sample_geo_accession = GSM3197623 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446300 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197624 !Sample_title = CY89FNAQ2_G05_S461_comb !Sample_geo_accession = GSM3197624 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446206 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197625 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_F05_S737_comb !Sample_geo_accession = GSM3197625 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446205 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197626 !Sample_title = cy60_1_cd_45_neg_3_F09_S549_comb !Sample_geo_accession = GSM3197626 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446204 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197627 !Sample_title = CY88_3_B08_S596_comb !Sample_geo_accession = GSM3197627 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446203 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197628 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_A11_S1067_comb !Sample_geo_accession = GSM3197628 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446202 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197629 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_E05_S53_comb !Sample_geo_accession = GSM3197629 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446201 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197630 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_E01_S145_comb !Sample_geo_accession = GSM3197630 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446200 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197631 !Sample_title = CY89NEG_B01_S13_comb !Sample_geo_accession = GSM3197631 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446199 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197632 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_G06_S1134_comb !Sample_geo_accession = GSM3197632 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446198 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197633 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_A11_S11_comb !Sample_geo_accession = GSM3197633 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446197 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197634 !Sample_title = CY88_3_G03_S651_comb !Sample_geo_accession = GSM3197634 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446271 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197635 !Sample_title = CY89FNAQ2_C06_S414_comb !Sample_geo_accession = GSM3197635 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446270 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197636 !Sample_title = Cy81_Bulk_CD45_B05_S113_comb !Sample_geo_accession = GSM3197636 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446269 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197637 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_G05_S77_comb !Sample_geo_accession = GSM3197637 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446268 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197638 !Sample_title = CY88_3_H08_S668_comb !Sample_geo_accession = GSM3197638 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446267 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197639 !Sample_title = Cy71_CD45_F07_S547_comb !Sample_geo_accession = GSM3197639 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446266 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197640 !Sample_title = cy80_CD45_neg_C03_S891_comb !Sample_geo_accession = GSM3197640 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446265 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197641 !Sample_title = CY89CORE11_B08_S116_comb !Sample_geo_accession = GSM3197641 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446264 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197642 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_G11_S83_comb !Sample_geo_accession = GSM3197642 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446263 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197643 !Sample_title = cy78_CD45_neg_1_A11_S491_comb !Sample_geo_accession = GSM3197643 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446262 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197644 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_B09_S693_comb !Sample_geo_accession = GSM3197644 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446261 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197645 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_G06_S366_comb !Sample_geo_accession = GSM3197645 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446260 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197646 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_G01_S361_comb !Sample_geo_accession = GSM3197646 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446259 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197647 !Sample_title = CY88_5_A05_S677_comb !Sample_geo_accession = GSM3197647 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446258 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197648 !Sample_title = CY89CORE11_E12_S156_comb !Sample_geo_accession = GSM3197648 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446257 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197649 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_D05_S809_comb !Sample_geo_accession = GSM3197649 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446255 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197650 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_C04_S796_comb !Sample_geo_accession = GSM3197650 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446254 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197651 !Sample_title = cy82_CD45_pos_2_E02_S530_comb !Sample_geo_accession = GSM3197651 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446253 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197652 !Sample_title = cy82_CD45_pos_2_G05_S557_comb !Sample_geo_accession = GSM3197652 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446252 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197653 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_F10_S70_comb !Sample_geo_accession = GSM3197653 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446429 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197654 !Sample_title = Cy80_II_CD45_C02_S890_comb !Sample_geo_accession = GSM3197654 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446428 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197655 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_F02_S62_comb !Sample_geo_accession = GSM3197655 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446256 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197656 !Sample_title = Cy80_II_CD45_F01_S925_comb !Sample_geo_accession = GSM3197656 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446427 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197657 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_G12_S1140_comb !Sample_geo_accession = GSM3197657 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446426 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197658 !Sample_title = CY88_3_B09_S597_comb !Sample_geo_accession = GSM3197658 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446384 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197659 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_E02_S50_comb !Sample_geo_accession = GSM3197659 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446458 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197660 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_C12_S132_comb !Sample_geo_accession = GSM3197660 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446457 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197661 !Sample_title = CY88_5_C01_S697_comb !Sample_geo_accession = GSM3197661 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446456 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197662 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_A06_S6_comb !Sample_geo_accession = GSM3197662 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446455 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197663 !Sample_title = CY89FNAQ2_D06_S426_comb !Sample_geo_accession = GSM3197663 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446454 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197664 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_B05_S113_comb !Sample_geo_accession = GSM3197664 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446453 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197665 !Sample_title = Cy80_II_CD45_E01_S913_comb !Sample_geo_accession = GSM3197665 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446452 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197666 !Sample_title = CY89NEG_G08_S80_comb !Sample_geo_accession = GSM3197666 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446451 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197667 !Sample_title = cy53_1_CD45_neg_A12_S300_comb !Sample_geo_accession = GSM3197667 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446450 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197668 !Sample_title = Cy81_FNA_CD45_B02_S302_comb !Sample_geo_accession = GSM3197668 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446449 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197669 !Sample_title = cy78_CD45_neg_2_C07_S607_comb !Sample_geo_accession = GSM3197669 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446448 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197670 !Sample_title = cy60_1_cd_45_neg_1_D11_S239_comb !Sample_geo_accession = GSM3197670 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446447 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197671 !Sample_title = CY88_3_E02_S626_comb !Sample_geo_accession = GSM3197671 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446446 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197672 !Sample_title = CY88_3_A12_S588_comb !Sample_geo_accession = GSM3197672 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446445 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197673 !Sample_title = cy80_CD45_neg_F01_S925_comb !Sample_geo_accession = GSM3197673 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446444 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197674 !Sample_title = cy81_Bulk_CD45_neg_G06_S174_comb !Sample_geo_accession = GSM3197674 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446443 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197675 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_G04_S460_comb !Sample_geo_accession = GSM3197675 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446442 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197676 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_B07_S211_comb !Sample_geo_accession = GSM3197676 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446441 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197677 !Sample_title = CY88_5_G02_S746_comb !Sample_geo_accession = GSM3197677 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446440 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197678 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_F10_S742_comb !Sample_geo_accession = GSM3197678 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446439 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197679 !Sample_title = cy80_CD45_neg_D02_S902_comb !Sample_geo_accession = GSM3197679 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446438 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197680 !Sample_title = CY89CORE11_F10_S166_comb !Sample_geo_accession = GSM3197680 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446435 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197681 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_B03_S399_comb !Sample_geo_accession = GSM3197681 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446434 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197682 !Sample_title = cy78_CD45_neg_1_C01_S505_comb !Sample_geo_accession = GSM3197682 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446433 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197683 !Sample_title = cy80_CD45_neg_B08_S884_comb !Sample_geo_accession = GSM3197683 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446432 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197684 !Sample_title = cy78_CD45_neg_2_A05_S581_comb !Sample_geo_accession = GSM3197684 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446431 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197685 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_D09_S717_comb !Sample_geo_accession = GSM3197685 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446437 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197686 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_F03_S255_comb !Sample_geo_accession = GSM3197686 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446436 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197687 !Sample_title = CY89FNAQ2_G04_S460_comb !Sample_geo_accession = GSM3197687 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446430 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197688 !Sample_title = cy80_CD45_neg_F08_S932_comb !Sample_geo_accession = GSM3197688 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446419 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197689 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_A02_S98_comb !Sample_geo_accession = GSM3197689 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446418 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197690 !Sample_title = Cy81_FNA_CD45_F07_S355_comb !Sample_geo_accession = GSM3197690 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446417 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197691 !Sample_title = CY88_3_G05_S653_comb !Sample_geo_accession = GSM3197691 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446416 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197692 !Sample_title = cy81_FNA_CD45_neg_E09_S1017_comb !Sample_geo_accession = GSM3197692 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446415 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197693 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_G09_S177_comb !Sample_geo_accession = GSM3197693 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446414 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197694 !Sample_title = Cy80_II_CD45_H11_S959_comb !Sample_geo_accession = GSM3197694 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446413 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197695 !Sample_title = cy78_CD45_neg_1_H05_S569_comb !Sample_geo_accession = GSM3197695 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446412 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197696 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_D02_S326_comb !Sample_geo_accession = GSM3197696 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446411 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197697 !Sample_title = Cy81_FNA_CD45_E04_S340_comb !Sample_geo_accession = GSM3197697 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446410 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197698 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_C04_S124_comb !Sample_geo_accession = GSM3197698 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446409 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197699 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_C04_S700_comb !Sample_geo_accession = GSM3197699 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446408 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197700 !Sample_title = cy82_CD45_pos_1_B11_S503_comb !Sample_geo_accession = GSM3197700 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446407 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197701 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_D09_S429_comb !Sample_geo_accession = GSM3197701 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446406 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197702 !Sample_title = cy78_CD45_neg_3_E06_S726_comb !Sample_geo_accession = GSM3197702 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446405 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197703 !Sample_title = cy82_CD45_pos_1_D01_S517_comb !Sample_geo_accession = GSM3197703 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446404 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197704 !Sample_title = cy82_CD45_neg_3_A11_S779_comb !Sample_geo_accession = GSM3197704 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446403 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197705 !Sample_title = CY89NEG_A10_S10_comb !Sample_geo_accession = GSM3197705 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446402 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197706 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_H05_S473_comb !Sample_geo_accession = GSM3197706 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446401 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197707 !Sample_title = Cy81_FNA_CD45_H09_S381_comb !Sample_geo_accession = GSM3197707 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446400 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197708 !Sample_title = CY88_3_E07_S631_comb !Sample_geo_accession = GSM3197708 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446399 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197709 !Sample_title = cy80_CD45_neg_E05_S917_comb !Sample_geo_accession = GSM3197709 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446398 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197710 !Sample_title = Cy80_II_CD45_A01_S865_comb !Sample_geo_accession = GSM3197710 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446397 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197711 !Sample_title = CY89NEG_A12_S12_comb !Sample_geo_accession = GSM3197711 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446396 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197712 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_B03_S111_comb !Sample_geo_accession = GSM3197712 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446395 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197713 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_G12_S276_comb !Sample_geo_accession = GSM3197713 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446394 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197714 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_C06_S414_comb !Sample_geo_accession = GSM3197714 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446393 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197715 !Sample_title = Cy81_Bulk_CD45_F12_S168_comb !Sample_geo_accession = GSM3197715 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446392 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197716 !Sample_title = cy81_Bulk_CD45_neg_H12_S192_comb !Sample_geo_accession = GSM3197716 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446391 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197717 !Sample_title = CY88_3_A08_S584_comb !Sample_geo_accession = GSM3197717 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446390 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197718 !Sample_title = cy81_FNA_CD45_neg_D01_S997_comb !Sample_geo_accession = GSM3197718 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446389 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197719 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_H09_S765_comb !Sample_geo_accession = GSM3197719 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446388 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197720 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_E03_S339_comb !Sample_geo_accession = GSM3197720 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446387 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197721 !Sample_title = Cy71_CD45_E06_S534_comb !Sample_geo_accession = GSM3197721 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446386 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197722 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_C08_S1088_comb !Sample_geo_accession = GSM3197722 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446385 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197723 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_A09_S777_comb !Sample_geo_accession = GSM3197723 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446425 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197724 !Sample_title = CY88_3_C06_S606_comb !Sample_geo_accession = GSM3197724 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446424 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197725 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_C03_S219_comb !Sample_geo_accession = GSM3197725 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446422 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197726 !Sample_title = cy81_FNA_CD45_neg_D03_S999_comb !Sample_geo_accession = GSM3197726 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446421 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197727 !Sample_title = cy81_FNA_CD45_neg_E06_S1014_comb !Sample_geo_accession = GSM3197727 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446484 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197728 !Sample_title = CY88_3_F11_S647_comb !Sample_geo_accession = GSM3197728 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446483 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197729 !Sample_title = CY89NEG_C10_S34_comb !Sample_geo_accession = GSM3197729 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446482 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197730 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_B07_S403_comb !Sample_geo_accession = GSM3197730 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446481 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197731 !Sample_title = cy81_FNA_CD45_neg_C11_S995_comb !Sample_geo_accession = GSM3197731 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446480 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197732 !Sample_title = CY88_5_A06_S678_comb !Sample_geo_accession = GSM3197732 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446479 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197733 !Sample_title = cy80_CD45_neg_B03_S879_comb !Sample_geo_accession = GSM3197733 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446478 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197734 !Sample_title = cy78_CD45_neg_3_C04_S700_comb !Sample_geo_accession = GSM3197734 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446477 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197735 !Sample_title = CY84_PRIM_POS_ALL_6_D06_S426_comb !Sample_geo_accession = GSM3197735 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446476 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197736 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_A06_S390_comb !Sample_geo_accession = GSM3197736 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446475 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197737 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_H05_S281_comb !Sample_geo_accession = GSM3197737 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446474 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197738 !Sample_title = Cy71_CD45_C12_S516_comb !Sample_geo_accession = GSM3197738 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446473 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197739 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_H05_S1145_comb !Sample_geo_accession = GSM3197739 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446472 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197740 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_C06_S126_comb !Sample_geo_accession = GSM3197740 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446471 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197741 !Sample_title = CY88_3_A11_S587_comb !Sample_geo_accession = GSM3197741 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446470 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197742 !Sample_title = CY88_3_F10_S646_comb !Sample_geo_accession = GSM3197742 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446469 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197743 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_C09_S129_comb !Sample_geo_accession = GSM3197743 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446463 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197744 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_E09_S1113_comb !Sample_geo_accession = GSM3197744 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446462 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197745 !Sample_title = CY88_3_G12_S660_comb !Sample_geo_accession = GSM3197745 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446468 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197746 !Sample_title = CY88_5_H04_S760_comb !Sample_geo_accession = GSM3197746 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446467 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197747 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_H01_S277_comb !Sample_geo_accession = GSM3197747 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446466 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197748 !Sample_title = CY89NEG_E03_S51_comb !Sample_geo_accession = GSM3197748 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446465 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197749 !Sample_title = cy82_CD45_pos_2_F02_S542_comb !Sample_geo_accession = GSM3197749 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446464 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197750 !Sample_title = CY88_3_G01_S649_comb !Sample_geo_accession = GSM3197750 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446461 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197751 !Sample_title = cy81_Bulk_CD45_neg_D01_S133_comb !Sample_geo_accession = GSM3197751 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446460 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197752 !Sample_title = cy78_CD45_neg_1_C06_S510_comb !Sample_geo_accession = GSM3197752 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446459 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197753 !Sample_title = Cy71_CD45_A06_S486_comb !Sample_geo_accession = GSM3197753 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446080 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197754 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_G08_S848_comb !Sample_geo_accession = GSM3197754 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446079 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197755 !Sample_title = Cy80_II_CD45_E11_S923_comb !Sample_geo_accession = GSM3197755 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446155 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197756 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_F04_S448_comb !Sample_geo_accession = GSM3197756 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446154 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197757 !Sample_title = CY88_5_F03_S735_comb !Sample_geo_accession = GSM3197757 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446153 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197758 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_C12_S324_comb !Sample_geo_accession = GSM3197758 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446152 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197759 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_A08_S104_comb !Sample_geo_accession = GSM3197759 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446151 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197760 !Sample_title = Cy71_CD45_C09_S513_comb !Sample_geo_accession = GSM3197760 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446150 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197761 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_G02_S74_comb !Sample_geo_accession = GSM3197761 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446149 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197762 !Sample_title = cy82_CD45_pos_2_D12_S528_comb !Sample_geo_accession = GSM3197762 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446148 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197763 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_D08_S1100_comb !Sample_geo_accession = GSM3197763 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446147 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197764 !Sample_title = Cy71_CD45_E05_S533_comb !Sample_geo_accession = GSM3197764 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446146 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197765 !Sample_title = cy84_Primary_CD45_neg_A10_S970_comb !Sample_geo_accession = GSM3197765 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446145 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197766 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_D03_S327_comb !Sample_geo_accession = GSM3197766 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446144 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197767 !Sample_title = CY84_PRIM_POS_ALL_6_G01_S457_comb !Sample_geo_accession = GSM3197767 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446143 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197768 !Sample_title = cy82_CD45_pos_3_A12_S12_comb !Sample_geo_accession = GSM3197768 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446142 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197769 !Sample_title = CY88_5_A03_S675_comb !Sample_geo_accession = GSM3197769 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446141 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197770 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_G07_S175_comb !Sample_geo_accession = GSM3197770 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446191 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197771 !Sample_title = Cy81_Bulk_CD45_A08_S104_comb !Sample_geo_accession = GSM3197771 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446190 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197772 !Sample_title = cy81_Bulk_CD45_neg_B02_S110_comb !Sample_geo_accession = GSM3197772 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446189 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197773 !Sample_title = cy80_CD45_neg_A06_S870_comb !Sample_geo_accession = GSM3197773 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446188 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197774 !Sample_title = cy78_CD45_neg_3_G10_S754_comb !Sample_geo_accession = GSM3197774 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446187 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197775 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_B12_S408_comb !Sample_geo_accession = GSM3197775 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446192 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197776 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_G09_S465_comb !Sample_geo_accession = GSM3197776 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446186 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197777 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_C01_S217_comb !Sample_geo_accession = GSM3197777 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446185 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197778 !Sample_title = Cy71_CD45_H12_S576_comb !Sample_geo_accession = GSM3197778 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446184 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197779 !Sample_title = cy81_Bulk_CD45_neg_B04_S112_comb !Sample_geo_accession = GSM3197779 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446597 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197780 !Sample_title = cy82_CD45_pos_3_H12_S96_comb !Sample_geo_accession = GSM3197780 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446183 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197781 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_H11_S863_comb !Sample_geo_accession = GSM3197781 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446182 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197782 !Sample_title = cy80_CD45_pos_PD1_pos_D09_S141_comb !Sample_geo_accession = GSM3197782 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446181 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197783 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_B12_S312_comb !Sample_geo_accession = GSM3197783 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446180 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197784 !Sample_title = cy78_CD45_neg_3_H04_S760_comb !Sample_geo_accession = GSM3197784 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446179 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197785 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_G11_S371_comb !Sample_geo_accession = GSM3197785 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446178 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197786 !Sample_title = CY88_5_F06_S738_comb !Sample_geo_accession = GSM3197786 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446177 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197787 !Sample_title = CY88_5_H06_S762_comb !Sample_geo_accession = GSM3197787 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446176 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197788 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_D02_S806_comb !Sample_geo_accession = GSM3197788 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446175 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197789 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_B07_S787_comb !Sample_geo_accession = GSM3197789 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446174 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197790 !Sample_title = Cy80_II_CD45_E08_S920_comb !Sample_geo_accession = GSM3197790 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446173 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197791 !Sample_title = cy78_CD45_neg_1_D04_S520_comb !Sample_geo_accession = GSM3197791 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446172 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197792 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_F01_S445_comb !Sample_geo_accession = GSM3197792 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446171 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197793 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_F08_S356_comb !Sample_geo_accession = GSM3197793 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446170 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197794 !Sample_title = Cy81_FNA_CD45_H05_S377_comb !Sample_geo_accession = GSM3197794 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446169 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197795 !Sample_title = cy82_CD45_pos_1_H12_S576_comb !Sample_geo_accession = GSM3197795 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446168 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197796 !Sample_title = cy81_FNA_CD45_neg_D12_S1008_comb !Sample_geo_accession = GSM3197796 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446167 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197797 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_A08_S776_comb !Sample_geo_accession = GSM3197797 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446166 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197798 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_B12_S1080_comb !Sample_geo_accession = GSM3197798 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446165 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197799 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_G02_S1130_comb !Sample_geo_accession = GSM3197799 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446164 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197800 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_C05_S701_comb !Sample_geo_accession = GSM3197800 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446163 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197801 !Sample_title = Cy81_FNA_CD45_D12_S336_comb !Sample_geo_accession = GSM3197801 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446140 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197802 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_G03_S363_comb !Sample_geo_accession = GSM3197802 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446139 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197803 !Sample_title = cy82_CD45_pos_3_A08_S8_comb !Sample_geo_accession = GSM3197803 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446138 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197804 !Sample_title = cy81_FNA_CD45_neg_E08_S1016_comb !Sample_geo_accession = GSM3197804 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446137 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197805 !Sample_title = cy81_Bulk_CD45_neg_A07_S103_comb !Sample_geo_accession = GSM3197805 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446136 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197806 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_E01_S721_comb !Sample_geo_accession = GSM3197806 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446135 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197807 !Sample_title = CY88_3_F09_S645_comb !Sample_geo_accession = GSM3197807 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446134 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197808 !Sample_title = cy78_CD45_neg_3_A11_S683_comb !Sample_geo_accession = GSM3197808 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446133 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197809 !Sample_title = cy94_cd45neg_cd90pos_D01_S325_comb !Sample_geo_accession = GSM3197809 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446132 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197810 !Sample_title = cy81_Bulk_CD45_neg_H07_S187_comb !Sample_geo_accession = GSM3197810 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446131 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197811 !Sample_title = CY88_5_A12_S684_comb !Sample_geo_accession = GSM3197811 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446130 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197812 !Sample_title = cy82_CD45_pos_2_D03_S519_comb !Sample_geo_accession = GSM3197812 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446129 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197813 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_A10_S1066_comb !Sample_geo_accession = GSM3197813 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446128 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197814 !Sample_title = cy78_CD45_neg_2_B05_S593_comb !Sample_geo_accession = GSM3197814 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446127 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197815 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_F08_S164_comb !Sample_geo_accession = GSM3197815 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446126 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197816 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_H04_S184_comb !Sample_geo_accession = GSM3197816 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446125 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197817 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_G10_S466_comb !Sample_geo_accession = GSM3197817 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446124 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197818 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_C09_S321_comb !Sample_geo_accession = GSM3197818 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446123 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197819 !Sample_title = Cy71_CD45_A07_S487_comb !Sample_geo_accession = GSM3197819 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446122 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197820 !Sample_title = Cy71_CD45_G11_S563_comb !Sample_geo_accession = GSM3197820 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446121 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197821 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_F10_S454_comb !Sample_geo_accession = GSM3197821 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446120 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197822 !Sample_title = CY88_3_G10_S658_comb !Sample_geo_accession = GSM3197822 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446119 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197823 !Sample_title = cy81_FNA_CD45_neg_E01_S1009_comb !Sample_geo_accession = GSM3197823 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446118 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197824 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_C03_S123_comb !Sample_geo_accession = GSM3197824 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446117 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197825 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_G06_S750_comb !Sample_geo_accession = GSM3197825 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446116 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197826 !Sample_title = cy81_FNA_CD45_neg_F01_S1021_comb !Sample_geo_accession = GSM3197826 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446115 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197827 !Sample_title = CY88_3_D11_S623_comb !Sample_geo_accession = GSM3197827 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446196 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197828 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_F12_S168_comb !Sample_geo_accession = GSM3197828 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446195 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197829 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_C02_S698_comb !Sample_geo_accession = GSM3197829 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446194 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197830 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_A02_S1058_comb !Sample_geo_accession = GSM3197830 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446193 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197831 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_D07_S715_comb !Sample_geo_accession = GSM3197831 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446285 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197832 !Sample_title = cy81_FNA_CD45_neg_H02_S1046_comb !Sample_geo_accession = GSM3197832 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446280 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197833 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_B01_S109_comb !Sample_geo_accession = GSM3197833 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446279 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197834 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_D08_S140_comb !Sample_geo_accession = GSM3197834 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446278 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197835 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_D10_S142_comb !Sample_geo_accession = GSM3197835 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446284 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197836 !Sample_title = cy81_FNA_CD45_neg_B03_S975_comb !Sample_geo_accession = GSM3197836 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446283 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197837 !Sample_title = cy81_Bulk_CD45_neg_H02_S182_comb !Sample_geo_accession = GSM3197837 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446282 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197838 !Sample_title = cy80_CD45_neg_A10_S874_comb !Sample_geo_accession = GSM3197838 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446281 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197839 !Sample_title = CY88_3_H04_S664_comb !Sample_geo_accession = GSM3197839 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446277 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197840 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_B06_S114_comb !Sample_geo_accession = GSM3197840 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446276 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197841 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_E04_S820_comb !Sample_geo_accession = GSM3197841 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446275 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197842 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_G10_S370_comb !Sample_geo_accession = GSM3197842 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446274 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197843 !Sample_title = cy78_CD45_neg_3_B10_S694_comb !Sample_geo_accession = GSM3197843 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446273 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197844 !Sample_title = cy53_1_CD45_neg_F07_S355_comb !Sample_geo_accession = GSM3197844 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446272 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197845 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_B06_S18_comb !Sample_geo_accession = GSM3197845 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446344 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197846 !Sample_title = Cy81_Bulk_CD45_F05_S161_comb !Sample_geo_accession = GSM3197846 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446343 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197847 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_C08_S320_comb !Sample_geo_accession = GSM3197847 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446342 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197848 !Sample_title = CY88_5_B02_S686_comb !Sample_geo_accession = GSM3197848 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446341 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197849 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_F09_S357_comb !Sample_geo_accession = GSM3197849 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446340 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197850 !Sample_title = cy60_1_cd_45_neg_1_C08_S224_comb !Sample_geo_accession = GSM3197850 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446339 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197851 !Sample_title = cy78_CD45_neg_3_G08_S752_comb !Sample_geo_accession = GSM3197851 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446338 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197852 !Sample_title = cy81_FNA_CD45_neg_E10_S1018_comb !Sample_geo_accession = GSM3197852 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446337 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197853 !Sample_title = cy78_CD45_neg_1_C05_S509_comb !Sample_geo_accession = GSM3197853 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446336 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197854 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_G02_S458_comb !Sample_geo_accession = GSM3197854 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446335 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197855 !Sample_title = Cy81_Bulk_CD45_D08_S140_comb !Sample_geo_accession = GSM3197855 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446334 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197856 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_G12_S84_comb !Sample_geo_accession = GSM3197856 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446333 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197857 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_E12_S348_comb !Sample_geo_accession = GSM3197857 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446332 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197858 !Sample_title = cy78_CD45_neg_3_D11_S719_comb !Sample_geo_accession = GSM3197858 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446331 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197859 !Sample_title = CY88_3_F06_S642_comb !Sample_geo_accession = GSM3197859 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446330 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197860 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_B08_S692_comb !Sample_geo_accession = GSM3197860 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446329 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197861 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_H07_S283_comb !Sample_geo_accession = GSM3197861 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446373 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197862 !Sample_title = Cy81_FNA_CD45_B04_S304_comb !Sample_geo_accession = GSM3197862 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446372 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197863 !Sample_title = cy78_CD45_neg_3_B05_S689_comb !Sample_geo_accession = GSM3197863 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446371 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197864 !Sample_title = CY88_3_D05_S617_comb !Sample_geo_accession = GSM3197864 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446364 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197865 !Sample_title = Cy80_II_CD45_H12_S960_comb !Sample_geo_accession = GSM3197865 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446370 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197866 !Sample_title = CY88_5_A07_S679_comb !Sample_geo_accession = GSM3197866 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446369 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197867 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_D07_S43_comb !Sample_geo_accession = GSM3197867 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446368 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197868 !Sample_title = cy78_CD45_neg_3_G03_S747_comb !Sample_geo_accession = GSM3197868 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446367 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197869 !Sample_title = cy81_FNA_CD45_neg_H10_S1054_comb !Sample_geo_accession = GSM3197869 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446366 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197870 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_H07_S1147_comb !Sample_geo_accession = GSM3197870 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446365 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197871 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_C10_S322_comb !Sample_geo_accession = GSM3197871 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446363 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197872 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_F03_S735_comb !Sample_geo_accession = GSM3197872 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446362 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197873 !Sample_title = CY88_5_A09_S681_comb !Sample_geo_accession = GSM3197873 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446361 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197874 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_B12_S696_comb !Sample_geo_accession = GSM3197874 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446360 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197875 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_D11_S47_comb !Sample_geo_accession = GSM3197875 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446359 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197876 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_H06_S378_comb !Sample_geo_accession = GSM3197876 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446358 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197877 !Sample_title = Cy81_FNA_CD45_H01_S373_comb !Sample_geo_accession = GSM3197877 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446357 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197878 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_D08_S812_comb !Sample_geo_accession = GSM3197878 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446356 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197879 !Sample_title = cy81_FNA_CD45_neg_H11_S1055_comb !Sample_geo_accession = GSM3197879 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446355 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197880 !Sample_title = cy84_Primary_CD45_neg_H02_S1046_comb !Sample_geo_accession = GSM3197880 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446354 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197881 !Sample_title = Cy71_CD45_C07_S511_comb !Sample_geo_accession = GSM3197881 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446353 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197882 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_G01_S169_comb !Sample_geo_accession = GSM3197882 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446352 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197883 !Sample_title = Cy81_Bulk_CD45_A12_S108_comb !Sample_geo_accession = GSM3197883 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446351 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197884 !Sample_title = CY88_3_A05_S581_comb !Sample_geo_accession = GSM3197884 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446350 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197885 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_A09_S105_comb !Sample_geo_accession = GSM3197885 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446349 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197886 !Sample_title = Cy80_II_CD45_A06_S870_comb !Sample_geo_accession = GSM3197886 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446348 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197887 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_F04_S1120_comb !Sample_geo_accession = GSM3197887 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446347 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197888 !Sample_title = CY88_3_E08_S632_comb !Sample_geo_accession = GSM3197888 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446346 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197889 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_D08_S236_comb !Sample_geo_accession = GSM3197889 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446345 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197890 !Sample_title = cy78_CD45_neg_1_B12_S504_comb !Sample_geo_accession = GSM3197890 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446420 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197891 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_C11_S131_comb !Sample_geo_accession = GSM3197891 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446162 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197892 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_E06_S54_comb !Sample_geo_accession = GSM3197892 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446161 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197893 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_D10_S718_comb !Sample_geo_accession = GSM3197893 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446160 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197894 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_E06_S342_comb !Sample_geo_accession = GSM3197894 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446159 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197895 !Sample_title = Cy81_Bulk_CD45_E06_S150_comb !Sample_geo_accession = GSM3197895 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446158 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197896 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_H11_S287_comb !Sample_geo_accession = GSM3197896 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446157 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197897 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_A08_S8_comb !Sample_geo_accession = GSM3197897 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446156 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197898 !Sample_title = Cy81_Bulk_CD45_A02_S98_comb !Sample_geo_accession = GSM3197898 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446229 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197899 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_A03_S3_comb !Sample_geo_accession = GSM3197899 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446228 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197900 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_F02_S254_comb !Sample_geo_accession = GSM3197900 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446227 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197901 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_H02_S1142_comb !Sample_geo_accession = GSM3197901 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446226 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197902 !Sample_title = cy78_CD45_neg_2_A10_S586_comb !Sample_geo_accession = GSM3197902 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446225 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197903 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_B09_S405_comb !Sample_geo_accession = GSM3197903 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446224 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197904 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_B11_S695_comb !Sample_geo_accession = GSM3197904 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446223 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197905 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_G08_S752_comb !Sample_geo_accession = GSM3197905 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446222 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197906 !Sample_title = Cy71_CD45_C10_S514_comb !Sample_geo_accession = GSM3197906 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446221 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197907 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_H12_S192_comb !Sample_geo_accession = GSM3197907 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446220 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197908 !Sample_title = Cy81_Bulk_CD45_D06_S138_comb !Sample_geo_accession = GSM3197908 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446219 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197909 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_H08_S860_comb !Sample_geo_accession = GSM3197909 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446218 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197910 !Sample_title = cy78_CD45_neg_3_F10_S742_comb !Sample_geo_accession = GSM3197910 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446217 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197911 !Sample_title = cy53_1_CD45_neg_C09_S321_comb !Sample_geo_accession = GSM3197911 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446216 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197912 !Sample_title = cy53_1_CD45_neg_F08_S356_comb !Sample_geo_accession = GSM3197912 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446215 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197913 !Sample_title = Cy71_CD45_C02_S506_comb !Sample_geo_accession = GSM3197913 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446214 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197914 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_H06_S186_comb !Sample_geo_accession = GSM3197914 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446213 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197915 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_D07_S235_comb !Sample_geo_accession = GSM3197915 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446212 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197916 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_A03_S291_comb !Sample_geo_accession = GSM3197916 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446211 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197917 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_C09_S33_comb !Sample_geo_accession = GSM3197917 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446210 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197918 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_D05_S233_comb !Sample_geo_accession = GSM3197918 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446209 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197919 !Sample_title = cy78_CD45_neg_3_A09_S681_comb !Sample_geo_accession = GSM3197919 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446208 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197920 !Sample_title = cy78_CD45_neg_1_A06_S486_comb !Sample_geo_accession = GSM3197920 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446207 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197921 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_E07_S439_comb !Sample_geo_accession = GSM3197921 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446110 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197922 !Sample_title = Cy71_CD45_F08_S548_comb !Sample_geo_accession = GSM3197922 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446109 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197923 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_A05_S389_comb !Sample_geo_accession = GSM3197923 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446108 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197924 !Sample_title = cy78_CD45_neg_1_F11_S551_comb !Sample_geo_accession = GSM3197924 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446107 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197925 !Sample_title = cy80_CD45_neg_C11_S899_comb !Sample_geo_accession = GSM3197925 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446106 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197926 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_E02_S338_comb !Sample_geo_accession = GSM3197926 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446105 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197927 !Sample_title = Cy71_CD45_C04_S508_comb !Sample_geo_accession = GSM3197927 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446104 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197928 !Sample_title = CY88_3_F08_S644_comb !Sample_geo_accession = GSM3197928 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446103 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197929 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_E12_S252_comb !Sample_geo_accession = GSM3197929 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446102 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197930 !Sample_title = Cy81_FNA_CD45_D11_S335_comb !Sample_geo_accession = GSM3197930 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446101 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197931 !Sample_title = cy80_CD45_neg_C07_S895_comb !Sample_geo_accession = GSM3197931 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446100 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197932 !Sample_title = CY88_5_D12_S720_comb !Sample_geo_accession = GSM3197932 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446099 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197933 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_A08_S680_comb !Sample_geo_accession = GSM3197933 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446098 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197934 !Sample_title = Cy71_CD45_H04_S568_comb !Sample_geo_accession = GSM3197934 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446097 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197935 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_H08_S188_comb !Sample_geo_accession = GSM3197935 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446096 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197936 !Sample_title = Cy81_FNA_CD45_C10_S322_comb !Sample_geo_accession = GSM3197936 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446095 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197937 !Sample_title = cy84_Primary_CD45_neg_H07_S1051_comb !Sample_geo_accession = GSM3197937 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446094 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197938 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_B01_S397_comb !Sample_geo_accession = GSM3197938 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446093 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197939 !Sample_title = Cy71_CD45_D01_S517_comb !Sample_geo_accession = GSM3197939 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446092 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197940 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_G01_S457_comb !Sample_geo_accession = GSM3197940 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446091 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197941 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_F07_S739_comb !Sample_geo_accession = GSM3197941 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446090 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197942 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_F08_S452_comb !Sample_geo_accession = GSM3197942 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446089 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197943 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_F09_S837_comb !Sample_geo_accession = GSM3197943 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446088 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197944 !Sample_title = CY89NEG_C02_S26_comb !Sample_geo_accession = GSM3197944 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446087 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197945 !Sample_title = cy81_Bulk_CD45_neg_E11_S155_comb !Sample_geo_accession = GSM3197945 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446086 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197946 !Sample_title = cy80_CD45_neg_B02_S878_comb !Sample_geo_accession = GSM3197946 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446085 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197947 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_B10_S214_comb !Sample_geo_accession = GSM3197947 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446084 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197948 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_B08_S212_comb !Sample_geo_accession = GSM3197948 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446083 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197949 !Sample_title = cy94_cd45neg_cd90pos_C09_S321_comb !Sample_geo_accession = GSM3197949 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446082 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197950 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_E04_S436_comb !Sample_geo_accession = GSM3197950 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446053 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197951 !Sample_title = Cy71_CD45_B09_S501_comb !Sample_geo_accession = GSM3197951 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446052 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197952 !Sample_title = CY88_5_B08_S692_comb !Sample_geo_accession = GSM3197952 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446051 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197953 !Sample_title = CY89NEG_H01_S85_comb !Sample_geo_accession = GSM3197953 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446050 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197954 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_E12_S732_comb !Sample_geo_accession = GSM3197954 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446049 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197955 !Sample_title = CY88_5_E11_S731_comb !Sample_geo_accession = GSM3197955 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446081 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197956 !Sample_title = cy80_CD45_neg_B12_S888_comb !Sample_geo_accession = GSM3197956 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446054 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197957 !Sample_title = CY75_1FNA_F07_S547_comb_1 !Sample_geo_accession = GSM3197957 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446048 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197958 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_A04_S100_comb !Sample_geo_accession = GSM3197958 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446047 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197959 !Sample_title = CY89CORE11_A07_S103_comb !Sample_geo_accession = GSM3197959 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446046 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197960 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_D10_S1102_comb !Sample_geo_accession = GSM3197960 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446045 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197961 !Sample_title = cy81_FNA_CD45_neg_C07_S991_comb !Sample_geo_accession = GSM3197961 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446044 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197962 !Sample_title = cy78_CD45_neg_3_A03_S675_comb !Sample_geo_accession = GSM3197962 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446114 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197963 !Sample_title = Cy81_Bulk_CD45_G06_S174_comb !Sample_geo_accession = GSM3197963 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446113 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197964 !Sample_title = Cy71_CD45_H11_S575_comb !Sample_geo_accession = GSM3197964 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446112 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197965 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_D01_S37_comb !Sample_geo_accession = GSM3197965 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446111 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197966 !Sample_title = cy94_cd45neg_cd90pos_D05_S329_comb !Sample_geo_accession = GSM3197966 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446596 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197967 !Sample_title = cy53_1_CD45_neg_B06_S306_comb !Sample_geo_accession = GSM3197967 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446595 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197968 !Sample_title = CY75_1FNA_A05_S485_comb_2 !Sample_geo_accession = GSM3197968 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446594 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197969 !Sample_title = CY88_3_A04_S580_comb !Sample_geo_accession = GSM3197969 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446593 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197970 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_C07_S31_comb !Sample_geo_accession = GSM3197970 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446592 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197971 !Sample_title = Cy71_CD45_D06_S522_comb !Sample_geo_accession = GSM3197971 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446591 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197972 !Sample_title = CY89NEG_B08_S20_comb !Sample_geo_accession = GSM3197972 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446590 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197973 !Sample_title = CY88_5_G08_S752_comb !Sample_geo_accession = GSM3197973 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446589 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197974 !Sample_title = cy53_1_CD45_neg_G03_S363_comb !Sample_geo_accession = GSM3197974 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446588 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197975 !Sample_title = CY88_5_C11_S707_comb !Sample_geo_accession = GSM3197975 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446587 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197976 !Sample_title = CY88_5_A10_S682_comb !Sample_geo_accession = GSM3197976 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446586 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197977 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_G11_S1139_comb !Sample_geo_accession = GSM3197977 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446585 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197978 !Sample_title = cy78_CD45_neg_1_C07_S511_comb !Sample_geo_accession = GSM3197978 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446584 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197979 !Sample_title = CY89CORE11_E04_S148_comb !Sample_geo_accession = GSM3197979 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446583 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197980 !Sample_title = cy80_CD45_neg_B10_S886_comb !Sample_geo_accession = GSM3197980 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446603 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197981 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_C02_S314_comb !Sample_geo_accession = GSM3197981 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446602 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197982 !Sample_title = CY88_5_D04_S712_comb !Sample_geo_accession = GSM3197982 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446601 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197983 !Sample_title = Cy80_II_CD45_C08_S896_comb !Sample_geo_accession = GSM3197983 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446600 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197984 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_G12_S372_comb !Sample_geo_accession = GSM3197984 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446599 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197985 !Sample_title = cy80_CD45_neg_A07_S871_comb !Sample_geo_accession = GSM3197985 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446598 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197986 !Sample_title = Cy71_CD45_H01_S565_comb !Sample_geo_accession = GSM3197986 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446574 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197987 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_B06_S1074_comb !Sample_geo_accession = GSM3197987 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446573 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197988 !Sample_title = cy78_CD45_neg_3_D06_S714_comb !Sample_geo_accession = GSM3197988 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446572 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197989 !Sample_title = cy78_CD45_neg_3_C10_S706_comb !Sample_geo_accession = GSM3197989 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446571 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197990 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_F12_S360_comb !Sample_geo_accession = GSM3197990 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446570 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197991 !Sample_title = cy80_CD45_neg_E04_S916_comb !Sample_geo_accession = GSM3197991 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446569 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197992 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_E10_S346_comb !Sample_geo_accession = GSM3197992 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446568 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197993 !Sample_title = Cy81_FNA_CD45_E01_S337_comb !Sample_geo_accession = GSM3197993 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446567 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197994 !Sample_title = CY89CORE11_H03_S183_comb !Sample_geo_accession = GSM3197994 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446566 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197995 !Sample_title = CY88_5_B04_S688_comb !Sample_geo_accession = GSM3197995 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446565 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197996 !Sample_title = Cy71_CD45_B10_S502_comb !Sample_geo_accession = GSM3197996 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446564 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197997 !Sample_title = CY88_3_H03_S663_comb !Sample_geo_accession = GSM3197997 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446563 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197998 !Sample_title = CY89NEG_H10_S94_comb !Sample_geo_accession = GSM3197998 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446562 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3197999 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_H10_S190_comb !Sample_geo_accession = GSM3197999 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446561 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198000 !Sample_title = Cy81_Bulk_CD45_D12_S144_comb !Sample_geo_accession = GSM3198000 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446560 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198001 !Sample_title = cy78_CD45_neg_3_C03_S699_comb !Sample_geo_accession = GSM3198001 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446559 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198002 !Sample_title = cy78_CD45_neg_3_H08_S764_comb !Sample_geo_accession = GSM3198002 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446558 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198003 !Sample_title = cy53_1_CD45_neg_A07_S295_comb !Sample_geo_accession = GSM3198003 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446616 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198004 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_B04_S400_comb !Sample_geo_accession = GSM3198004 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446615 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198005 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_G10_S178_comb !Sample_geo_accession = GSM3198005 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446614 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198006 !Sample_title = CY89NEG_C08_S32_comb !Sample_geo_accession = GSM3198006 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446613 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198007 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_A02_S290_comb !Sample_geo_accession = GSM3198007 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446612 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198008 !Sample_title = Cy71_CD45_F05_S545_comb !Sample_geo_accession = GSM3198008 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446611 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198009 !Sample_title = cy81_Bulk_CD45_neg_D05_S137_comb !Sample_geo_accession = GSM3198009 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446610 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198010 !Sample_title = cy82_CD45_pos_3_G06_S78_comb !Sample_geo_accession = GSM3198010 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446609 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198011 !Sample_title = CY88_5_G12_S756_comb !Sample_geo_accession = GSM3198011 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446605 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198012 !Sample_title = CY88_5_C07_S703_comb !Sample_geo_accession = GSM3198012 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446604 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198013 !Sample_title = cy80_CD45_neg_C01_S889_comb !Sample_geo_accession = GSM3198013 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447303 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198014 !Sample_title = cy81_Bulk_CD45_neg_E10_S154_comb !Sample_geo_accession = GSM3198014 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447302 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198015 !Sample_title = cy81_Bulk_CD45_neg_H11_S191_comb !Sample_geo_accession = GSM3198015 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446608 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198016 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_H12_S768_comb !Sample_geo_accession = GSM3198016 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446607 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198017 !Sample_title = CY89CORE11_G10_S178_comb !Sample_geo_accession = GSM3198017 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446606 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198018 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_G03_S171_comb !Sample_geo_accession = GSM3198018 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447301 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198019 !Sample_title = CY89FNAQ2_G02_S458_comb !Sample_geo_accession = GSM3198019 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447300 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198020 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_E03_S51_comb !Sample_geo_accession = GSM3198020 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447299 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198021 !Sample_title = Cy81_Bulk_CD45_G03_S171_comb !Sample_geo_accession = GSM3198021 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447298 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198022 !Sample_title = CY88_5_H01_S757_comb !Sample_geo_accession = GSM3198022 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447297 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198023 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_A04_S1060_comb !Sample_geo_accession = GSM3198023 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447296 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198024 !Sample_title = cy60_1_cd_45_neg_3_H06_S570_comb !Sample_geo_accession = GSM3198024 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447295 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198025 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_G04_S76_comb !Sample_geo_accession = GSM3198025 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447294 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198026 !Sample_title = cy78_CD45_neg_3_B04_S688_comb !Sample_geo_accession = GSM3198026 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447293 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198027 !Sample_title = cy84_Primary_CD45_neg_A07_S967_comb !Sample_geo_accession = GSM3198027 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447292 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198028 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_C05_S1085_comb !Sample_geo_accession = GSM3198028 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447291 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198029 !Sample_title = cy81_Bulk_CD45_neg_D10_S142_comb !Sample_geo_accession = GSM3198029 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447290 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198030 !Sample_title = cy53_1_CD45_neg_D05_S329_comb !Sample_geo_accession = GSM3198030 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447289 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198031 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_B05_S1073_comb !Sample_geo_accession = GSM3198031 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447209 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198032 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_D02_S710_comb !Sample_geo_accession = GSM3198032 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447208 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198033 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_G01_S265_comb !Sample_geo_accession = GSM3198033 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447207 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198034 !Sample_title = Cy81_Bulk_CD45_D11_S143_comb !Sample_geo_accession = GSM3198034 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447284 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198035 !Sample_title = CY89FNAQ2_H05_S473_comb !Sample_geo_accession = GSM3198035 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447283 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198036 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_G09_S1137_comb !Sample_geo_accession = GSM3198036 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447282 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198037 !Sample_title = CY88_5_E08_S728_comb !Sample_geo_accession = GSM3198037 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447281 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198038 !Sample_title = cy84_Primary_CD45_neg_F05_S1025_comb !Sample_geo_accession = GSM3198038 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447280 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198039 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_B01_S13_comb !Sample_geo_accession = GSM3198039 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447279 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198040 !Sample_title = cy78_CD45_neg_3_A08_S680_comb !Sample_geo_accession = GSM3198040 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447278 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198041 !Sample_title = cy81_Bulk_CD45_neg_F02_S158_comb !Sample_geo_accession = GSM3198041 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447277 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198042 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_D09_S333_comb !Sample_geo_accession = GSM3198042 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447276 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198043 !Sample_title = cy78_CD45_neg_3_F08_S740_comb !Sample_geo_accession = GSM3198043 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447275 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198044 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_C12_S804_comb !Sample_geo_accession = GSM3198044 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447274 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198045 !Sample_title = Cy80_II_CD45_G03_S939_comb !Sample_geo_accession = GSM3198045 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447273 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198046 !Sample_title = CY88_5_C04_S700_comb !Sample_geo_accession = GSM3198046 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447272 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198047 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_G05_S1133_comb !Sample_geo_accession = GSM3198047 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447271 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198048 !Sample_title = cy84_Primary_CD45_neg_H12_S1056_comb !Sample_geo_accession = GSM3198048 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447270 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198049 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_E03_S1107_comb !Sample_geo_accession = GSM3198049 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447269 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198050 !Sample_title = cy78_CD45_neg_3_G06_S750_comb !Sample_geo_accession = GSM3198050 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447268 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198051 !Sample_title = cy81_FNA_CD45_neg_G07_S1039_comb !Sample_geo_accession = GSM3198051 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447267 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198052 !Sample_title = cy80_CD45_neg_A12_S876_comb !Sample_geo_accession = GSM3198052 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447266 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198053 !Sample_title = cy78_CD45_neg_3_E10_S730_comb !Sample_geo_accession = GSM3198053 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447265 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198054 !Sample_title = cy81_FNA_CD45_neg_G05_S1037_comb !Sample_geo_accession = GSM3198054 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447264 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198055 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_G02_S842_comb !Sample_geo_accession = GSM3198055 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447263 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198056 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_C04_S220_comb !Sample_geo_accession = GSM3198056 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447262 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198057 !Sample_title = cy53_1_CD45_neg_H11_S383_comb !Sample_geo_accession = GSM3198057 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447261 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198058 !Sample_title = cy81_Bulk_CD45_neg_H03_S183_comb !Sample_geo_accession = GSM3198058 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447260 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198059 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_A10_S778_comb !Sample_geo_accession = GSM3198059 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447259 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198060 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_F11_S263_comb !Sample_geo_accession = GSM3198060 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447258 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198061 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_E06_S438_comb !Sample_geo_accession = GSM3198061 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447257 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198062 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_H12_S864_comb !Sample_geo_accession = GSM3198062 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447256 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198063 !Sample_title = Cy71_CD45_C03_S507_comb !Sample_geo_accession = GSM3198063 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447255 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198064 !Sample_title = cy60_1_cd_45_neg_3_G10_S562_comb !Sample_geo_accession = GSM3198064 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447254 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198065 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_F05_S449_comb !Sample_geo_accession = GSM3198065 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447253 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198066 !Sample_title = Cy71_CD45_H08_S572_comb !Sample_geo_accession = GSM3198066 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447252 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198067 !Sample_title = cy78_CD45_neg_1_G01_S553_comb !Sample_geo_accession = GSM3198067 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447251 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198068 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_H02_S758_comb !Sample_geo_accession = GSM3198068 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447250 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198069 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_B05_S17_comb !Sample_geo_accession = GSM3198069 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447249 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198070 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_G10_S754_comb !Sample_geo_accession = GSM3198070 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447248 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198071 !Sample_title = CY89NEG_G05_S77_comb !Sample_geo_accession = GSM3198071 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447321 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198072 !Sample_title = Cy81_FNA_CD45_B12_S312_comb !Sample_geo_accession = GSM3198072 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447320 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198073 !Sample_title = Cy81_FNA_CD45_H04_S376_comb !Sample_geo_accession = GSM3198073 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447319 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198074 !Sample_title = CY89CORE11_G11_S179_comb !Sample_geo_accession = GSM3198074 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447318 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198075 !Sample_title = CY88CD45_150813_B05_S305_comb_3 !Sample_geo_accession = GSM3198075 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447247 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198076 !Sample_title = CY89CORE11_B07_S115_comb !Sample_geo_accession = GSM3198076 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447246 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198077 !Sample_title = cy60_1_cd_45_neg_1_D07_S235_comb !Sample_geo_accession = GSM3198077 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447317 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198078 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_D01_S229_comb !Sample_geo_accession = GSM3198078 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447316 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198079 !Sample_title = cy78_CD45_neg_3_B11_S695_comb !Sample_geo_accession = GSM3198079 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447315 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198080 !Sample_title = Cy81_Bulk_CD45_D04_S136_comb !Sample_geo_accession = GSM3198080 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447314 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198081 !Sample_title = Cy81_FNA_CD45_H08_S380_comb !Sample_geo_accession = GSM3198081 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447313 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198082 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_D12_S48_comb !Sample_geo_accession = GSM3198082 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447312 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198083 !Sample_title = Cy71_CD45_B11_S503_comb !Sample_geo_accession = GSM3198083 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447311 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198084 !Sample_title = CY89CORE11_D03_S135_comb !Sample_geo_accession = GSM3198084 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447310 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198085 !Sample_title = Cy71_CD45_H02_S566_comb !Sample_geo_accession = GSM3198085 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447309 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198086 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_E02_S242_comb !Sample_geo_accession = GSM3198086 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447308 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198087 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_D08_S44_comb !Sample_geo_accession = GSM3198087 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447307 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198088 !Sample_title = CY89CORE11_D04_S136_comb !Sample_geo_accession = GSM3198088 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447306 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198089 !Sample_title = CY88_5_C10_S706_comb !Sample_geo_accession = GSM3198089 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447305 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198090 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_E09_S441_comb !Sample_geo_accession = GSM3198090 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447304 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198091 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_D06_S426_comb !Sample_geo_accession = GSM3198091 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447467 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198092 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_C07_S415_comb !Sample_geo_accession = GSM3198092 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447466 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198093 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_E03_S819_comb !Sample_geo_accession = GSM3198093 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447465 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198094 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_F06_S738_comb !Sample_geo_accession = GSM3198094 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447464 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198095 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_D11_S239_comb !Sample_geo_accession = GSM3198095 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447463 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198096 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_B02_S110_comb !Sample_geo_accession = GSM3198096 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447462 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198097 !Sample_title = Cy80_II_CD45_C01_S889_comb !Sample_geo_accession = GSM3198097 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447461 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198098 !Sample_title = cy78_CD45_neg_1_F07_S547_comb !Sample_geo_accession = GSM3198098 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447460 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198099 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_C09_S417_comb !Sample_geo_accession = GSM3198099 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447459 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198100 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_F10_S358_comb !Sample_geo_accession = GSM3198100 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447458 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198101 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_H05_S857_comb !Sample_geo_accession = GSM3198101 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447457 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198102 !Sample_title = CY88_5_B05_S689_comb !Sample_geo_accession = GSM3198102 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447456 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198103 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_H02_S182_comb !Sample_geo_accession = GSM3198103 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447455 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198104 !Sample_title = CY89FNAQ2_F08_S452_comb !Sample_geo_accession = GSM3198104 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447454 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198105 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_A06_S294_comb !Sample_geo_accession = GSM3198105 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447453 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198106 !Sample_title = Cy71_CD45_G03_S555_comb !Sample_geo_accession = GSM3198106 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447452 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198107 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_E11_S443_comb !Sample_geo_accession = GSM3198107 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447451 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198108 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_A12_S300_comb !Sample_geo_accession = GSM3198108 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447450 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198109 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_F09_S69_comb !Sample_geo_accession = GSM3198109 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447449 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198110 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_G04_S364_comb !Sample_geo_accession = GSM3198110 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447448 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198111 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_A03_S771_comb !Sample_geo_accession = GSM3198111 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447447 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198112 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_G05_S365_comb !Sample_geo_accession = GSM3198112 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447446 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198113 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_F05_S257_comb !Sample_geo_accession = GSM3198113 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447445 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198114 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_B09_S309_comb !Sample_geo_accession = GSM3198114 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447444 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198115 !Sample_title = Cy80_II_CD45_E12_S924_comb !Sample_geo_accession = GSM3198115 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447443 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198116 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_G06_S174_comb !Sample_geo_accession = GSM3198116 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447442 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198117 !Sample_title = cy80_CD45_neg_G06_S942_comb !Sample_geo_accession = GSM3198117 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447441 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198118 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_F09_S165_comb !Sample_geo_accession = GSM3198118 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447440 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198119 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_A05_S101_comb !Sample_geo_accession = GSM3198119 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447439 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198120 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_E06_S822_comb !Sample_geo_accession = GSM3198120 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447438 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198121 !Sample_title = Cy80_II_CD45_B08_S884_comb !Sample_geo_accession = GSM3198121 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447429 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198122 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_E02_S722_comb !Sample_geo_accession = GSM3198122 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447428 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198123 !Sample_title = Cy80_II_CD45_E06_S918_comb !Sample_geo_accession = GSM3198123 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447427 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198124 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_C06_S318_comb !Sample_geo_accession = GSM3198124 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447426 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198125 !Sample_title = CY88_3_E06_S630_comb !Sample_geo_accession = GSM3198125 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447425 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198126 !Sample_title = Cy71_CD45_E08_S536_comb !Sample_geo_accession = GSM3198126 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447424 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198127 !Sample_title = cy82_CD45_pos_3_E02_S50_comb !Sample_geo_accession = GSM3198127 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447423 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198128 !Sample_title = cy78_CD45_neg_1_C03_S507_comb !Sample_geo_accession = GSM3198128 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447422 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198129 !Sample_title = cy80_CD45_neg_G09_S945_comb !Sample_geo_accession = GSM3198129 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447421 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198130 !Sample_title = CY89NEG_E04_S52_comb !Sample_geo_accession = GSM3198130 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447420 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198131 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_C11_S35_comb !Sample_geo_accession = GSM3198131 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447419 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198132 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_F11_S1127_comb !Sample_geo_accession = GSM3198132 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447418 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198133 !Sample_title = CY88CD45POS_2_B11_S407_comb !Sample_geo_accession = GSM3198133 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447413 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198134 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_B07_S307_comb !Sample_geo_accession = GSM3198134 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447412 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198135 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_D12_S240_comb !Sample_geo_accession = GSM3198135 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447417 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198136 !Sample_title = Cy71_CD45_B06_S498_comb !Sample_geo_accession = GSM3198136 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447416 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198137 !Sample_title = CY88_3_A01_S577_comb !Sample_geo_accession = GSM3198137 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447415 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198138 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_D07_S811_comb !Sample_geo_accession = GSM3198138 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447414 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198139 !Sample_title = cy82_CD45_pos_3_G12_S84_comb !Sample_geo_accession = GSM3198139 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447411 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198140 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_D03_S711_comb !Sample_geo_accession = GSM3198140 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447410 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198141 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_B11_S791_comb !Sample_geo_accession = GSM3198141 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447409 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198142 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_E07_S727_comb !Sample_geo_accession = GSM3198142 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447408 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198143 !Sample_title = cy78_CD45_neg_1_D06_S522_comb !Sample_geo_accession = GSM3198143 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447407 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198144 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_D12_S336_comb !Sample_geo_accession = GSM3198144 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447406 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198145 !Sample_title = cy82_CD45_pos_1_G08_S560_comb !Sample_geo_accession = GSM3198145 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447405 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198146 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_B01_S205_comb !Sample_geo_accession = GSM3198146 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447404 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198147 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_C10_S130_comb !Sample_geo_accession = GSM3198147 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447471 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198148 !Sample_title = CY84_PRIM_POS_ALL_6_E04_S436_comb !Sample_geo_accession = GSM3198148 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447470 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198149 !Sample_title = cy81_FNA_CD45_neg_B07_S979_comb !Sample_geo_accession = GSM3198149 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447469 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198150 !Sample_title = cy78_CD45_neg_3_F11_S743_comb !Sample_geo_accession = GSM3198150 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447468 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198151 !Sample_title = CY89NEG_D08_S44_comb !Sample_geo_accession = GSM3198151 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447332 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198152 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_F08_S68_comb !Sample_geo_accession = GSM3198152 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447331 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198153 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_C12_S708_comb !Sample_geo_accession = GSM3198153 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447330 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198154 !Sample_title = cy81_Bulk_CD45_neg_H08_S188_comb !Sample_geo_accession = GSM3198154 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447329 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198155 !Sample_title = CY88_5_H11_S767_comb !Sample_geo_accession = GSM3198155 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447328 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198156 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_B01_S1069_comb !Sample_geo_accession = GSM3198156 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447327 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198157 !Sample_title = CY89FNAQ2_C08_S416_comb !Sample_geo_accession = GSM3198157 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447326 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198158 !Sample_title = CY89CORE11_E08_S152_comb !Sample_geo_accession = GSM3198158 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447325 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198159 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_D06_S42_comb !Sample_geo_accession = GSM3198159 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447324 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198160 !Sample_title = CY89NEG_F12_S72_comb !Sample_geo_accession = GSM3198160 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447323 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198161 !Sample_title = CY89NEG_A08_S8_comb !Sample_geo_accession = GSM3198161 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447322 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198162 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_H07_S763_comb !Sample_geo_accession = GSM3198162 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447403 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198163 !Sample_title = cy78_CD45_neg_3_A07_S679_comb !Sample_geo_accession = GSM3198163 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447402 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198164 !Sample_title = Cy80_II_CD45_A02_S866_comb !Sample_geo_accession = GSM3198164 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447401 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198165 !Sample_title = cy80_CD45_neg_F11_S935_comb !Sample_geo_accession = GSM3198165 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447400 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198166 !Sample_title = Cy80_II_CD45_D07_S907_comb !Sample_geo_accession = GSM3198166 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447399 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198167 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_E07_S55_comb !Sample_geo_accession = GSM3198167 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447397 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198168 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_B02_S398_comb !Sample_geo_accession = GSM3198168 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447396 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198169 !Sample_title = CY89CORE11_G05_S173_comb !Sample_geo_accession = GSM3198169 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447395 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198170 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_E03_S435_comb !Sample_geo_accession = GSM3198170 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447394 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198171 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_G11_S179_comb !Sample_geo_accession = GSM3198171 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447393 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198172 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_E07_S343_comb !Sample_geo_accession = GSM3198172 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447392 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198173 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_C12_S228_comb !Sample_geo_accession = GSM3198173 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447391 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198174 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_F02_S158_comb !Sample_geo_accession = GSM3198174 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447390 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198175 !Sample_title = CY89NEG_G07_S79_comb !Sample_geo_accession = GSM3198175 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447389 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198176 !Sample_title = cy78_CD45_neg_1_H10_S574_comb !Sample_geo_accession = GSM3198176 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447388 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198177 !Sample_title = CY89CORE11_F06_S162_comb !Sample_geo_accession = GSM3198177 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447387 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198178 !Sample_title = CY88_5_E10_S730_comb !Sample_geo_accession = GSM3198178 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447386 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198179 !Sample_title = cy78_CD45_neg_1_G02_S554_comb !Sample_geo_accession = GSM3198179 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447385 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198180 !Sample_title = CY89NEG_F06_S66_comb !Sample_geo_accession = GSM3198180 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447384 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198181 !Sample_title = cy81_Bulk_CD45_neg_C01_S121_comb !Sample_geo_accession = GSM3198181 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447383 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198182 !Sample_title = Cy81_FNA_CD45_F10_S358_comb !Sample_geo_accession = GSM3198182 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447382 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198183 !Sample_title = cy80_CD45_neg_E09_S921_comb !Sample_geo_accession = GSM3198183 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447381 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198184 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_G05_S269_comb !Sample_geo_accession = GSM3198184 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447380 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198185 !Sample_title = CY88_5_G11_S755_comb !Sample_geo_accession = GSM3198185 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447379 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198186 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_E05_S341_comb !Sample_geo_accession = GSM3198186 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447378 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198187 !Sample_title = CY89NEG_E10_S58_comb !Sample_geo_accession = GSM3198187 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447377 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198188 !Sample_title = cy82_CD45_pos_3_A10_S10_comb !Sample_geo_accession = GSM3198188 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447376 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198189 !Sample_title = CY89NEG_H12_S96_comb !Sample_geo_accession = GSM3198189 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447375 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198190 !Sample_title = cy80_CD45_neg_G08_S944_comb !Sample_geo_accession = GSM3198190 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447374 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198191 !Sample_title = Cy80_II_CD45_F10_S934_comb !Sample_geo_accession = GSM3198191 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447373 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198192 !Sample_title = cy82_CD45_pos_2_E03_S531_comb !Sample_geo_accession = GSM3198192 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447372 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198193 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_G10_S274_comb !Sample_geo_accession = GSM3198193 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447371 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198194 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_D02_S230_comb !Sample_geo_accession = GSM3198194 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447370 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198195 !Sample_title = CY88_5_D01_S709_comb !Sample_geo_accession = GSM3198195 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447369 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198196 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_E05_S1109_comb !Sample_geo_accession = GSM3198196 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447368 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198197 !Sample_title = Cy81_Bulk_CD45_G02_S170_comb !Sample_geo_accession = GSM3198197 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447367 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198198 !Sample_title = Cy80_II_CD45_D12_S912_comb !Sample_geo_accession = GSM3198198 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447366 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198199 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_F10_S166_comb !Sample_geo_accession = GSM3198199 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447365 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198200 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_B12_S216_comb !Sample_geo_accession = GSM3198200 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447364 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198201 !Sample_title = cy81_Bulk_CD45_neg_A02_S98_comb !Sample_geo_accession = GSM3198201 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447363 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198202 !Sample_title = Cy71_CD45_A03_S483_comb !Sample_geo_accession = GSM3198202 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447362 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198203 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_C01_S25_comb !Sample_geo_accession = GSM3198203 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447361 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198204 !Sample_title = cy60_1_cd_45_neg_1_B11_S215_comb !Sample_geo_accession = GSM3198204 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447360 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198205 !Sample_title = CY89CORE11_C02_S122_comb !Sample_geo_accession = GSM3198205 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447435 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198206 !Sample_title = cy81_Bulk_CD45_neg_E12_S156_comb !Sample_geo_accession = GSM3198206 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447434 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198207 !Sample_title = Cy81_FNA_CD45_C02_S314_comb !Sample_geo_accession = GSM3198207 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447433 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198208 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_A09_S201_comb !Sample_geo_accession = GSM3198208 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447432 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198209 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_H11_S383_comb !Sample_geo_accession = GSM3198209 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447431 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198210 !Sample_title = CY88_3_G04_S652_comb !Sample_geo_accession = GSM3198210 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447430 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198211 !Sample_title = CY89NEG_C04_S28_comb !Sample_geo_accession = GSM3198211 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447484 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198212 !Sample_title = Cy81_Bulk_CD45_E01_S145_comb !Sample_geo_accession = GSM3198212 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447483 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198213 !Sample_title = CY89CORE11_A10_S106_comb !Sample_geo_accession = GSM3198213 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447482 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198214 !Sample_title = CY88_5_H07_S763_comb !Sample_geo_accession = GSM3198214 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447481 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198215 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_A09_S393_comb !Sample_geo_accession = GSM3198215 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447479 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198216 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_B11_S23_comb !Sample_geo_accession = GSM3198216 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447478 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198217 !Sample_title = CY88_3_F04_S640_comb !Sample_geo_accession = GSM3198217 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447477 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198218 !Sample_title = CY89NEG_G11_S83_comb !Sample_geo_accession = GSM3198218 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447476 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198219 !Sample_title = CY88_5_G10_S754_comb !Sample_geo_accession = GSM3198219 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447475 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198220 !Sample_title = CY88_5_C03_S699_comb !Sample_geo_accession = GSM3198220 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447474 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198221 !Sample_title = cy78_CD45_neg_3_A10_S682_comb !Sample_geo_accession = GSM3198221 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447473 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198222 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_D04_S40_comb !Sample_geo_accession = GSM3198222 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447557 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198223 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_F06_S1122_comb !Sample_geo_accession = GSM3198223 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447552 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198224 !Sample_title = cy78_CD45_neg_1_E09_S537_comb !Sample_geo_accession = GSM3198224 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447551 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198225 !Sample_title = CY88_3_E11_S635_comb !Sample_geo_accession = GSM3198225 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447556 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198226 !Sample_title = Cy80_II_CD45_C04_S892_comb !Sample_geo_accession = GSM3198226 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447555 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198227 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_D02_S1094_comb !Sample_geo_accession = GSM3198227 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447554 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198228 !Sample_title = Cy81_Bulk_CD45_C11_S131_comb !Sample_geo_accession = GSM3198228 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447553 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198229 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_C02_S26_comb !Sample_geo_accession = GSM3198229 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447550 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198230 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_D01_S133_comb !Sample_geo_accession = GSM3198230 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447549 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198231 !Sample_title = Cy71_CD45_B01_S493_comb !Sample_geo_accession = GSM3198231 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447548 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198232 !Sample_title = CY89COREQ1_A09_S297_comb !Sample_geo_accession = GSM3198232 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447547 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198233 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_A09_S297_comb !Sample_geo_accession = GSM3198233 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447546 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198234 !Sample_title = Cy71_CD45_E09_S537_comb !Sample_geo_accession = GSM3198234 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447545 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198235 !Sample_title = cy82_CD45_pos_3_C01_S25_comb !Sample_geo_accession = GSM3198235 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447544 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198236 !Sample_title = cy81_FNA_CD45_neg_C06_S990_comb !Sample_geo_accession = GSM3198236 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447543 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198237 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_H03_S183_comb !Sample_geo_accession = GSM3198237 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447542 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198238 !Sample_title = cy81_FNA_CD45_neg_H03_S1047_comb !Sample_geo_accession = GSM3198238 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447541 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198239 !Sample_title = Cy81_FNA_CD45_C07_S319_comb !Sample_geo_accession = GSM3198239 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447540 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198240 !Sample_title = cy78_CD45_neg_1_C04_S508_comb !Sample_geo_accession = GSM3198240 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447539 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198241 !Sample_title = Cy80_II_CD45_B10_S886_comb !Sample_geo_accession = GSM3198241 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447437 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198242 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_E08_S344_comb !Sample_geo_accession = GSM3198242 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447436 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198243 !Sample_title = cy78_CD45_neg_3_A04_S676_comb !Sample_geo_accession = GSM3198243 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447512 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198244 !Sample_title = Cy71_CD45_G12_S564_comb !Sample_geo_accession = GSM3198244 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447511 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198245 !Sample_title = cy78_CD45_neg_3_G12_S756_comb !Sample_geo_accession = GSM3198245 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447510 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198246 !Sample_title = CY75_1FNA_G06_S558_comb_4 !Sample_geo_accession = GSM3198246 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447509 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198247 !Sample_title = CY88_5_F10_S742_comb !Sample_geo_accession = GSM3198247 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447508 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198248 !Sample_title = cy84_Primary_CD45_neg_D05_S1001_comb !Sample_geo_accession = GSM3198248 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447507 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198249 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_G01_S841_comb !Sample_geo_accession = GSM3198249 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447506 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198250 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_H05_S185_comb !Sample_geo_accession = GSM3198250 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447505 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198251 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_B05_S689_comb !Sample_geo_accession = GSM3198251 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447504 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198252 !Sample_title = CY88_5_B09_S693_comb !Sample_geo_accession = GSM3198252 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447503 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198253 !Sample_title = cy78_CD45_neg_3_E04_S724_comb !Sample_geo_accession = GSM3198253 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447502 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198254 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_F01_S61_comb !Sample_geo_accession = GSM3198254 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447501 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198255 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_C07_S703_comb !Sample_geo_accession = GSM3198255 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447500 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198256 !Sample_title = Cy81_FNA_CD45_F05_S353_comb !Sample_geo_accession = GSM3198256 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447499 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198257 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_C01_S409_comb !Sample_geo_accession = GSM3198257 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447498 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198258 !Sample_title = CY89FNAQ2_A09_S393_comb !Sample_geo_accession = GSM3198258 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447497 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198259 !Sample_title = CY88_3_G06_S654_comb !Sample_geo_accession = GSM3198259 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447496 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198260 !Sample_title = cy78_CD45_neg_3_H09_S765_comb !Sample_geo_accession = GSM3198260 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447495 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198261 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_D10_S430_comb !Sample_geo_accession = GSM3198261 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447494 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198262 !Sample_title = cy81_Bulk_CD45_neg_E07_S151_comb !Sample_geo_accession = GSM3198262 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447493 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198263 !Sample_title = CY89COREQ1_C11_S323_comb !Sample_geo_accession = GSM3198263 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447492 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198264 !Sample_title = cy79_p4_CD45_pos_PD1_pos_G06_S366_comb !Sample_geo_accession = GSM3198264 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447491 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198265 !Sample_title = cy80_Cd45_pos_PD1_pos_B09_S45_comb !Sample_geo_accession = GSM3198265 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447490 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198266 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_G10_S466_comb !Sample_geo_accession = GSM3198266 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447489 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198267 !Sample_title = cy74_CD45_pos_A12_S684_comb !Sample_geo_accession = GSM3198267 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447488 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198268 !Sample_title = CY89A_CD45_POS_10_C12_S228_comb !Sample_geo_accession = GSM3198268 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447487 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198269 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_G11_S467_comb !Sample_geo_accession = GSM3198269 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447486 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198270 !Sample_title = cy79_p4_CD45_pos_PD1_pos_C04_S316_comb !Sample_geo_accession = GSM3198270 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447485 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198271 !Sample_title = CY58_1_CD45_B02_S974_comb !Sample_geo_accession = GSM3198271 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447538 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198272 !Sample_title = cy79_p5_CD45_pos_PD1_pos_G10_S658_comb !Sample_geo_accession = GSM3198272 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447537 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198273 !Sample_title = cy80_CD45_neg_F02_S446_comb !Sample_geo_accession = GSM3198273 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447536 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198274 !Sample_title = cy80_CD45_pos_PD1_pos_E05_S149_comb !Sample_geo_accession = GSM3198274 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447535 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198275 !Sample_title = cy72_CD45_pos_H11_S959_comb !Sample_geo_accession = GSM3198275 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447534 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198276 !Sample_title = cy58_1_CD45_pos_D08_S620_comb !Sample_geo_accession = GSM3198276 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447533 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198277 !Sample_title = cy60_1_cd_45_pos_4_B04_S16_comb !Sample_geo_accession = GSM3198277 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447532 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198278 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_D09_S1101_comb !Sample_geo_accession = GSM3198278 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447531 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198279 !Sample_title = cy72_CD45_pos_C08_S896_comb !Sample_geo_accession = GSM3198279 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447530 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198280 !Sample_title = CY89A_Core_15_E01_S49_comb !Sample_geo_accession = GSM3198280 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447529 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198281 !Sample_title = CY88CD45POS_2_C11_S419_comb !Sample_geo_accession = GSM3198281 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447528 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198282 !Sample_title = cy82_CD45_pos_2_B09_S501_comb !Sample_geo_accession = GSM3198282 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447527 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198283 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_D05_S233_comb !Sample_geo_accession = GSM3198283 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447526 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198284 !Sample_title = cy60_1_cd_45_pos_4_G06_S78_comb !Sample_geo_accession = GSM3198284 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447525 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198285 !Sample_title = CY89COREQ1_A02_S290_comb !Sample_geo_accession = GSM3198285 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447524 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198286 !Sample_title = cy60_1_cd_45_pos_3_C06_S318_comb !Sample_geo_accession = GSM3198286 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447523 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198287 !Sample_title = CY94CD45POS_1_G06_S174_comb !Sample_geo_accession = GSM3198287 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447522 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198288 !Sample_title = cy80_CD45_pos_PD1_pos_F11_S167_comb !Sample_geo_accession = GSM3198288 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447521 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198289 !Sample_title = Cy72_CD45_D09_S717_comb !Sample_geo_accession = GSM3198289 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447520 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198290 !Sample_title = cy58_1_CD45_pos_F12_S648_comb !Sample_geo_accession = GSM3198290 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447519 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198291 !Sample_title = cy79_p5_CD45_pos_PD1_pos_C10_S610_comb !Sample_geo_accession = GSM3198291 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447518 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198292 !Sample_title = cy74_CD45_pos_E12_S732_comb !Sample_geo_accession = GSM3198292 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447517 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198293 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_G01_S265_comb !Sample_geo_accession = GSM3198293 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447516 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198294 !Sample_title = cy60_1_cd_45_pos_4_B10_S22_comb !Sample_geo_accession = GSM3198294 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447515 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198295 !Sample_title = CY88CD45_150813_E07_S343_comb !Sample_geo_accession = GSM3198295 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447514 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198296 !Sample_title = cy72_CD45_pos_B07_S883_comb !Sample_geo_accession = GSM3198296 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447513 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198297 !Sample_title = CY88CD45POS_2_E03_S435_comb !Sample_geo_accession = GSM3198297 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447561 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198298 !Sample_title = Cy74_CD45_A03_S387_comb !Sample_geo_accession = GSM3198298 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447560 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198299 !Sample_title = CY89A_CD45_POS_10_B02_S206_comb !Sample_geo_accession = GSM3198299 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447559 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198300 !Sample_title = CY89A_CD45_POS_10_H05_S281_comb !Sample_geo_accession = GSM3198300 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447558 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198301 !Sample_title = Cy74_CD45_F09_S453_comb !Sample_geo_accession = GSM3198301 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447193 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198302 !Sample_title = cy53_1_CD45_pos_2_F05_S1025_comb !Sample_geo_accession = GSM3198302 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447192 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198303 !Sample_title = cy80_CD45_neg_A09_S393_comb !Sample_geo_accession = GSM3198303 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447191 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198304 !Sample_title = CY58_1_CD45_D03_S999_comb !Sample_geo_accession = GSM3198304 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447190 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198305 !Sample_title = cy84_Primary_CD45_pos_C05_S413_comb !Sample_geo_accession = GSM3198305 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447189 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198306 !Sample_title = cy60_1_cd_45_pos_4_A06_S6_comb !Sample_geo_accession = GSM3198306 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447188 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198307 !Sample_title = CY89COREQ1_A04_S292_comb !Sample_geo_accession = GSM3198307 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447187 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198308 !Sample_title = cy79_p5_CD45_pos_PD1_pos_C01_S601_comb !Sample_geo_accession = GSM3198308 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447186 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198309 !Sample_title = CY89A_CD45_POS_6_B03_S111_comb !Sample_geo_accession = GSM3198309 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447185 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198310 !Sample_title = cy60_1_cd_45_pos_4_G12_S84_comb !Sample_geo_accession = GSM3198310 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447184 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198311 !Sample_title = CY89A_CD45_POS_10_G12_S276_comb !Sample_geo_accession = GSM3198311 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447183 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198312 !Sample_title = cy94_cd45pos_4_D05_S41_comb !Sample_geo_accession = GSM3198312 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447182 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198313 !Sample_title = cy79_p4_CD45_pos_PD1_pos_A09_S297_comb !Sample_geo_accession = GSM3198313 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447181 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198314 !Sample_title = Cy72_CD45_C01_S697_comb !Sample_geo_accession = GSM3198314 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447180 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198315 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_G09_S561_comb !Sample_geo_accession = GSM3198315 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447179 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198316 !Sample_title = cy60_1_cd_45_pos_3_H11_S383_comb !Sample_geo_accession = GSM3198316 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447178 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198317 !Sample_title = Cy71_CD45_D07_S523_comb !Sample_geo_accession = GSM3198317 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447177 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198318 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_C08_S416_comb !Sample_geo_accession = GSM3198318 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447176 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198319 !Sample_title = CY94CD45POS_1_D11_S143_comb !Sample_geo_accession = GSM3198319 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447175 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198320 !Sample_title = cy60_1_cd_45_pos_3_A05_S293_comb !Sample_geo_accession = GSM3198320 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447174 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198321 !Sample_title = cy74_CD45_pos_D09_S717_comb !Sample_geo_accession = GSM3198321 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447173 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198322 !Sample_title = Cy74_CD45_D04_S424_comb !Sample_geo_accession = GSM3198322 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447172 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198323 !Sample_title = cy88_cd_45_pos_G03_S459_comb !Sample_geo_accession = GSM3198323 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447171 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198324 !Sample_title = CY89COREQ1_E09_S345_comb !Sample_geo_accession = GSM3198324 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447170 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198325 !Sample_title = CY89COREQ1_B07_S307_comb !Sample_geo_accession = GSM3198325 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447169 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198326 !Sample_title = cy80_CD45_neg_H12_S480_comb !Sample_geo_accession = GSM3198326 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447245 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198327 !Sample_title = CY75_1FNA_E07_S535_comb !Sample_geo_accession = GSM3198327 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447244 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198328 !Sample_title = CY89A_CD45_POS_6_A07_S103_comb !Sample_geo_accession = GSM3198328 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447243 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198329 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_H01_S277_comb !Sample_geo_accession = GSM3198329 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447242 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198330 !Sample_title = cy72_CD45_pos_D03_S903_comb !Sample_geo_accession = GSM3198330 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447241 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198331 !Sample_title = Cy74_CD45_C11_S419_comb !Sample_geo_accession = GSM3198331 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446516 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198332 !Sample_title = cy79_p4_CD45_pos_PD1_pos_B03_S303_comb !Sample_geo_accession = GSM3198332 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446515 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198333 !Sample_title = CY88CD45_150813_D04_S328_comb !Sample_geo_accession = GSM3198333 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446514 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198334 !Sample_title = CY89A_CD45_POS_10_A04_S196_comb !Sample_geo_accession = GSM3198334 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446513 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198335 !Sample_title = cy60_1_cd_45_pos_4_D03_S39_comb !Sample_geo_accession = GSM3198335 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446512 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198336 !Sample_title = cy58_1_CD45_pos_D10_S622_comb !Sample_geo_accession = GSM3198336 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446511 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198337 !Sample_title = cy84_Primary_CD45_pos_A09_S393_comb !Sample_geo_accession = GSM3198337 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446510 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198338 !Sample_title = cy84_Met_CD45_pos_C08_S896_comb !Sample_geo_accession = GSM3198338 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446509 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198339 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_B10_S406_comb !Sample_geo_accession = GSM3198339 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446508 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198340 !Sample_title = Cy81_FNA_CD45_D07_S235_comb !Sample_geo_accession = GSM3198340 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446507 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198341 !Sample_title = Cy72_CD45_D07_S715_comb !Sample_geo_accession = GSM3198341 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446506 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198342 !Sample_title = cy79_p4_CD45_pos_PD1_pos_G07_S367_comb !Sample_geo_accession = GSM3198342 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446505 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198343 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_H08_S572_comb !Sample_geo_accession = GSM3198343 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446504 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198344 !Sample_title = cy80_CD45_neg_C11_S419_comb !Sample_geo_accession = GSM3198344 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446503 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198345 !Sample_title = Cy72_CD45_E08_S728_comb !Sample_geo_accession = GSM3198345 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446502 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198346 !Sample_title = cy72_CD45_pos_B03_S879_comb !Sample_geo_accession = GSM3198346 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446501 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198347 !Sample_title = cy84_Primary_CD45_pos_E05_S437_comb !Sample_geo_accession = GSM3198347 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446500 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198348 !Sample_title = cy80_CD45_pos_PD1_pos_G05_S173_comb !Sample_geo_accession = GSM3198348 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446499 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198349 !Sample_title = Cy71_CD45_B07_S499_comb !Sample_geo_accession = GSM3198349 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446498 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198350 !Sample_title = cy94_cd45pos_A05_S101_comb !Sample_geo_accession = GSM3198350 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446497 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198351 !Sample_title = cy53_1_CD45_pos_1_H03_S87_comb !Sample_geo_accession = GSM3198351 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446496 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198352 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_C02_S218_comb !Sample_geo_accession = GSM3198352 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446495 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198353 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_C08_S224_comb !Sample_geo_accession = GSM3198353 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446494 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198354 !Sample_title = cy72_CD45_pos_G10_S946_comb !Sample_geo_accession = GSM3198354 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446493 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198355 !Sample_title = cy84_Primary_CD45_pos_H09_S477_comb !Sample_geo_accession = GSM3198355 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446492 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198356 !Sample_title = cy72_CD45_pos_F07_S931_comb !Sample_geo_accession = GSM3198356 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446491 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198357 !Sample_title = cy53_1_CD45_pos_1_E06_S54_comb !Sample_geo_accession = GSM3198357 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446490 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198358 !Sample_title = cy79_p5_CD45_pos_PD1_pos_E08_S632_comb !Sample_geo_accession = GSM3198358 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446489 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198359 !Sample_title = cy58_1_CD45_pos_D06_S618_comb !Sample_geo_accession = GSM3198359 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446488 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198360 !Sample_title = cy80_CD45_pos_PD1_pos_E04_S148_comb !Sample_geo_accession = GSM3198360 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446487 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198361 !Sample_title = cy60_1_cd_45_pos_3_F04_S352_comb !Sample_geo_accession = GSM3198361 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447287 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198362 !Sample_title = cy72_CD45_pos_A06_S870_comb !Sample_geo_accession = GSM3198362 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447286 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198363 !Sample_title = CY89FNA_C01_S217_comb !Sample_geo_accession = GSM3198363 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447285 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198364 !Sample_title = cy74_CD45_pos_G03_S747_comb !Sample_geo_accession = GSM3198364 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447359 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198365 !Sample_title = CY89COREQ1_H09_S381_comb !Sample_geo_accession = GSM3198365 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447358 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198366 !Sample_title = Cy71_CD45_E03_S531_comb !Sample_geo_accession = GSM3198366 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447357 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198367 !Sample_title = Cy74_CD45_H03_S471_comb !Sample_geo_accession = GSM3198367 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447356 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198368 !Sample_title = CY89A_CD45_POS_6_C07_S127_comb !Sample_geo_accession = GSM3198368 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447355 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198369 !Sample_title = cy80_Cd45_pos_PD1_pos_B11_S47_comb !Sample_geo_accession = GSM3198369 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447354 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198370 !Sample_title = cy72_CD45_pos_C02_S890_comb !Sample_geo_accession = GSM3198370 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447353 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198371 !Sample_title = cy80_Cd45_pos_PD1_pos_S189_H09_S189_comb !Sample_geo_accession = GSM3198371 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447352 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198372 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_H10_S286_comb !Sample_geo_accession = GSM3198372 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447351 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198373 !Sample_title = cy80_CD45_neg_E11_S443_comb !Sample_geo_accession = GSM3198373 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447350 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198374 !Sample_title = cy88_cd_45_pos_F11_S455_comb !Sample_geo_accession = GSM3198374 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447349 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198375 !Sample_title = CY58_1_CD45_A06_S966_comb !Sample_geo_accession = GSM3198375 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447348 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198376 !Sample_title = cy94_cd45pos_4_D11_S47_comb !Sample_geo_accession = GSM3198376 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447347 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198377 !Sample_title = cy53_1_CD45_pos_1_B11_S23_comb !Sample_geo_accession = GSM3198377 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447346 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198378 !Sample_title = CY89COREQ1_F11_S359_comb !Sample_geo_accession = GSM3198378 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447345 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198379 !Sample_title = cy79_p4_CD45_pos_PD1_pos_A04_S292_comb !Sample_geo_accession = GSM3198379 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447344 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198380 !Sample_title = cy74_CD45_pos_B04_S688_comb !Sample_geo_accession = GSM3198380 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447343 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198381 !Sample_title = Cy81_FNA_CD45_A09_S201_comb !Sample_geo_accession = GSM3198381 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447342 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198382 !Sample_title = CY89A_CD45_POS_6_B11_S119_comb !Sample_geo_accession = GSM3198382 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447341 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198383 !Sample_title = CY94CD45POS_1_B11_S119_comb !Sample_geo_accession = GSM3198383 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447340 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198384 !Sample_title = Cy81_FNA_CD45_B12_S216_comb !Sample_geo_accession = GSM3198384 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447339 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198385 !Sample_title = Cy71_CD45_B08_S500_comb !Sample_geo_accession = GSM3198385 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447338 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198386 !Sample_title = cy72_CD45_pos_E07_S919_comb !Sample_geo_accession = GSM3198386 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447337 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198387 !Sample_title = cy84_Primary_CD45_pos_A06_S390_comb !Sample_geo_accession = GSM3198387 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447336 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198388 !Sample_title = cy72_CD45_pos_H10_S958_comb !Sample_geo_accession = GSM3198388 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447335 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198389 !Sample_title = cy80_Cd45_pos_PD1_pos_A05_S17_comb !Sample_geo_accession = GSM3198389 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447334 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198390 !Sample_title = cy94_cd45pos_F04_S160_comb !Sample_geo_accession = GSM3198390 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447333 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198391 !Sample_title = cy88_cd_45_pos_3_C01_S601_comb !Sample_geo_accession = GSM3198391 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446538 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198392 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_F08_S452_comb !Sample_geo_accession = GSM3198392 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446537 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198393 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_C10_S994_comb !Sample_geo_accession = GSM3198393 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446536 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198394 !Sample_title = cy80_Cd45_pos_PD1_pos_C02_S62_comb !Sample_geo_accession = GSM3198394 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446535 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198395 !Sample_title = cy58_1_CD45_pos_B02_S590_comb !Sample_geo_accession = GSM3198395 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446534 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198396 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_C01_S409_comb !Sample_geo_accession = GSM3198396 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446533 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198397 !Sample_title = cy72_CD45_pos_A08_S872_comb !Sample_geo_accession = GSM3198397 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446532 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198398 !Sample_title = cy79_p5_CD45_pos_PD1_pos_A04_S580_comb !Sample_geo_accession = GSM3198398 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446531 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198399 !Sample_title = cy80_Cd45_pos_PD1_pos_S165_G09_S165_comb !Sample_geo_accession = GSM3198399 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446530 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198400 !Sample_title = cy80_Cd45_pos_PD1_pos_S115_E07_S115_comb !Sample_geo_accession = GSM3198400 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446529 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198401 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_A08_S392_comb !Sample_geo_accession = GSM3198401 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446528 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198402 !Sample_title = cy74_CD45_pos_C05_S701_comb !Sample_geo_accession = GSM3198402 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446527 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198403 !Sample_title = cy80_Cd45_pos_PD1_pos_C07_S67_comb !Sample_geo_accession = GSM3198403 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446526 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198404 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_D12_S432_comb !Sample_geo_accession = GSM3198404 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446520 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198405 !Sample_title = CY58_1_CD45_D11_S1007_comb !Sample_geo_accession = GSM3198405 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446525 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198406 !Sample_title = CY94CD45POS_1_H08_S188_comb !Sample_geo_accession = GSM3198406 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446524 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198407 !Sample_title = cy58_1_CD45_pos_A07_S583_comb !Sample_geo_accession = GSM3198407 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446523 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198408 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_H03_S279_comb !Sample_geo_accession = GSM3198408 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446522 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198409 !Sample_title = Cy72_CD45_F12_S744_comb !Sample_geo_accession = GSM3198409 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446521 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198410 !Sample_title = cy74_CD45_pos_A09_S681_comb !Sample_geo_accession = GSM3198410 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446519 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198411 !Sample_title = cy74_CD45_pos_D02_S710_comb !Sample_geo_accession = GSM3198411 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446518 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198412 !Sample_title = cy94_cd45neg_cd90pos_G06_S366_comb !Sample_geo_accession = GSM3198412 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446517 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198413 !Sample_title = cy79_p5_CD45_pos_PD1_pos_F06_S642_comb !Sample_geo_accession = GSM3198413 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446582 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198414 !Sample_title = cy72_CD45_pos_E08_S920_comb !Sample_geo_accession = GSM3198414 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446581 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198415 !Sample_title = cy80_CD45_pos_PD1_pos_F05_S161_comb !Sample_geo_accession = GSM3198415 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446580 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198416 !Sample_title = cy60_1_cd_45_pos_4_C03_S27_comb !Sample_geo_accession = GSM3198416 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446579 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198417 !Sample_title = cy84_Primary_CD45_pos_G06_S462_comb !Sample_geo_accession = GSM3198417 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446578 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198418 !Sample_title = cy84_Primary_CD45_pos_C01_S409_comb !Sample_geo_accession = GSM3198418 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446577 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198419 !Sample_title = Cy72_CD45_C07_S703_comb !Sample_geo_accession = GSM3198419 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446576 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198420 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_C04_S412_comb !Sample_geo_accession = GSM3198420 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446575 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198421 !Sample_title = cy94_cd45pos_G03_S171_comb !Sample_geo_accession = GSM3198421 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447240 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198422 !Sample_title = cy72_CD45_pos_H01_S949_comb !Sample_geo_accession = GSM3198422 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447239 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198423 !Sample_title = cy94_cd45pos_D12_S144_comb !Sample_geo_accession = GSM3198423 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447238 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198424 !Sample_title = cy80_CD45_pos_PD1_pos_G01_S169_comb !Sample_geo_accession = GSM3198424 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447237 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198425 !Sample_title = cy80_CD45_pos_PD1_pos_B11_S119_comb !Sample_geo_accession = GSM3198425 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447236 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198426 !Sample_title = CY89A_CD45_POS_6_G11_S179_comb !Sample_geo_accession = GSM3198426 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447235 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198427 !Sample_title = cy84_Met_CD45_pos_G05_S941_comb !Sample_geo_accession = GSM3198427 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447234 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198428 !Sample_title = cy74_CD45_pos_F12_S744_comb !Sample_geo_accession = GSM3198428 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447233 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198429 !Sample_title = cy80_CD45_pos_PD1_pos_D12_S144_comb !Sample_geo_accession = GSM3198429 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447232 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198430 !Sample_title = cy79_p4_CD45_pos_PD1_pos_C11_S323_comb !Sample_geo_accession = GSM3198430 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447231 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198431 !Sample_title = cy88_cd_45_pos_3_F07_S643_comb !Sample_geo_accession = GSM3198431 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447230 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198432 !Sample_title = cy84_Met_CD45_pos_F09_S933_comb !Sample_geo_accession = GSM3198432 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447229 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198433 !Sample_title = cy80_CD45_neg_B11_S407_comb !Sample_geo_accession = GSM3198433 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447228 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198434 !Sample_title = CY89A_CD45_POS_6_F02_S158_comb !Sample_geo_accession = GSM3198434 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447227 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198435 !Sample_title = cy58_1_CD45_pos_H02_S662_comb !Sample_geo_accession = GSM3198435 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447226 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198436 !Sample_title = cy58_1_CD45_pos_A04_S580_comb !Sample_geo_accession = GSM3198436 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447225 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198437 !Sample_title = cy80_Cd45_pos_Pd1_neg_S196_A04_S196_comb !Sample_geo_accession = GSM3198437 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447223 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198438 !Sample_title = cy80_Cd45_pos_PD1_pos_S120_E12_S120_comb !Sample_geo_accession = GSM3198438 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447222 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198439 !Sample_title = Cy74_CD45_F07_S451_comb !Sample_geo_accession = GSM3198439 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447221 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198440 !Sample_title = cy74_CD45_pos_B06_S690_comb !Sample_geo_accession = GSM3198440 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447220 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198441 !Sample_title = CY89A_CD45_POS_6_H11_S191_comb !Sample_geo_accession = GSM3198441 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447219 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198442 !Sample_title = cy79_p5_CD45_pos_PD1_pos_B04_S592_comb !Sample_geo_accession = GSM3198442 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447218 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198443 !Sample_title = cy79_p5_CD45_pos_PD1_pos_F10_S646_comb !Sample_geo_accession = GSM3198443 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447217 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198444 !Sample_title = CY89A_Core_15_F02_S62_comb !Sample_geo_accession = GSM3198444 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447216 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198445 !Sample_title = cy80_CD45_pos_PD1_pos_H06_S186_comb !Sample_geo_accession = GSM3198445 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447215 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198446 !Sample_title = Cy81_FNA_CD45_B04_S208_comb !Sample_geo_accession = GSM3198446 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447214 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198447 !Sample_title = cy79_p3_CD45_pos_PD1_neg_E04_S148_comb !Sample_geo_accession = GSM3198447 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447213 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198448 !Sample_title = cy80_Cd45_pos_Pd1_neg_S272_D08_S272_comb !Sample_geo_accession = GSM3198448 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447212 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198449 !Sample_title = cy58_1_CD45_pos_C12_S612_comb !Sample_geo_accession = GSM3198449 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447211 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198450 !Sample_title = CY89COREQ1_D09_S333_comb !Sample_geo_accession = GSM3198450 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447210 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198451 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_D01_S997_comb !Sample_geo_accession = GSM3198451 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447090 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198452 !Sample_title = cy80_CD45_pos_PD1_pos_F03_S159_comb !Sample_geo_accession = GSM3198452 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447089 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198453 !Sample_title = Cy74_CD45_C02_S410_comb !Sample_geo_accession = GSM3198453 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447088 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198454 !Sample_title = CY89A_Core_15_F11_S71_comb !Sample_geo_accession = GSM3198454 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447087 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198455 !Sample_title = Cy81_FNA_CD45_G01_S265_comb !Sample_geo_accession = GSM3198455 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447086 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198456 !Sample_title = Cy81_FNA_CD45_D02_S230_comb !Sample_geo_accession = GSM3198456 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447085 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198457 !Sample_title = cy79_p4_CD45_pos_PD1_pos_A03_S291_comb !Sample_geo_accession = GSM3198457 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447084 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198458 !Sample_title = cy60_1_cd_45_pos_4_C05_S29_comb !Sample_geo_accession = GSM3198458 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447083 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198459 !Sample_title = cy72_CD45_pos_F03_S927_comb !Sample_geo_accession = GSM3198459 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447082 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198460 !Sample_title = cy80_Cd45_pos_PD1_pos_S159_G03_S159_comb !Sample_geo_accession = GSM3198460 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447081 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198461 !Sample_title = cy74_CD45_pos_C09_S705_comb !Sample_geo_accession = GSM3198461 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447080 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198462 !Sample_title = CY58_1_CD45_A10_S970_comb !Sample_geo_accession = GSM3198462 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447079 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198463 !Sample_title = cy82_CD45_pos_2_G03_S555_comb !Sample_geo_accession = GSM3198463 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447078 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198464 !Sample_title = Cy72_CD45_D12_S720_comb !Sample_geo_accession = GSM3198464 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447077 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198465 !Sample_title = CY89A_Core_15_H06_S90_comb !Sample_geo_accession = GSM3198465 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447076 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198466 !Sample_title = cy79_p4_CD45_pos_PD1_pos_D08_S332_comb !Sample_geo_accession = GSM3198466 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447075 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198467 !Sample_title = cy94_cd45pos_4_E02_S50_comb !Sample_geo_accession = GSM3198467 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447074 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198468 !Sample_title = cy80_CD45_pos_PD1_pos_B07_S115_comb !Sample_geo_accession = GSM3198468 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447073 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198469 !Sample_title = CY58_1_CD45_F11_S1031_comb !Sample_geo_accession = GSM3198469 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447072 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198470 !Sample_title = cy53_1_CD45_pos_2_A05_S965_comb !Sample_geo_accession = GSM3198470 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447071 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198471 !Sample_title = cy72_CD45_pos_B09_S885_comb !Sample_geo_accession = GSM3198471 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447070 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198472 !Sample_title = cy72_CD45_pos_A07_S871_comb !Sample_geo_accession = GSM3198472 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447069 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198473 !Sample_title = cy80_CD45_pos_PD1_pos_G10_S178_comb !Sample_geo_accession = GSM3198473 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447068 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198474 !Sample_title = cy94_cd45neg_cd90pos_B11_S311_comb !Sample_geo_accession = GSM3198474 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447067 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198475 !Sample_title = cy79_p5_CD45_pos_PD1_pos_D02_S614_comb !Sample_geo_accession = GSM3198475 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447066 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198476 !Sample_title = CY89FNA_G11_S275_comb !Sample_geo_accession = GSM3198476 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447065 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198477 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_C11_S419_comb !Sample_geo_accession = GSM3198477 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447064 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198478 !Sample_title = cy84_Primary_CD45_pos_D07_S427_comb !Sample_geo_accession = GSM3198478 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447063 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198479 !Sample_title = cy80_CD45_pos_PD1_pos_G07_S175_comb !Sample_geo_accession = GSM3198479 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447061 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198480 !Sample_title = cy88_cd_45_pos_C03_S411_comb !Sample_geo_accession = GSM3198480 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447060 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198481 !Sample_title = CY89A_Core_15_C07_S31_comb !Sample_geo_accession = GSM3198481 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447045 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198482 !Sample_title = cy84_Primary_CD45_pos_B04_S400_comb !Sample_geo_accession = GSM3198482 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447044 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198483 !Sample_title = cy72_CD45_pos_A12_S876_comb !Sample_geo_accession = GSM3198483 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447043 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198484 !Sample_title = Cy81_FNA_CD45_F01_S253_comb !Sample_geo_accession = GSM3198484 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447042 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198485 !Sample_title = cy80_Cd45_pos_PD1_pos_S138_F06_S138_comb !Sample_geo_accession = GSM3198485 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447041 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198486 !Sample_title = Cy74_CD45_A08_S392_comb !Sample_geo_accession = GSM3198486 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447040 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198487 !Sample_title = Cy71_CD45_G06_S558_comb !Sample_geo_accession = GSM3198487 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447039 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198488 !Sample_title = Cy74_CD45_A04_S388_comb !Sample_geo_accession = GSM3198488 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447038 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198489 !Sample_title = CY58_1_CD45_B01_S973_comb !Sample_geo_accession = GSM3198489 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447037 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198490 !Sample_title = cy94_cd45pos_4_E12_S60_comb !Sample_geo_accession = GSM3198490 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447036 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198491 !Sample_title = CY89A_CD45_POS_6_B12_S120_comb !Sample_geo_accession = GSM3198491 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447033 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198492 !Sample_title = CY89COREQ1_F09_S357_comb !Sample_geo_accession = GSM3198492 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447032 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198493 !Sample_title = cy84_Primary_CD45_pos_E02_S434_comb !Sample_geo_accession = GSM3198493 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447031 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198494 !Sample_title = CY94CD45POS_1_B10_S118_comb !Sample_geo_accession = GSM3198494 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447030 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198495 !Sample_title = CY94CD45POS_1_E01_S145_comb !Sample_geo_accession = GSM3198495 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447035 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198496 !Sample_title = CY75_1FNA_G09_S561_comb !Sample_geo_accession = GSM3198496 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447034 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198497 !Sample_title = CY58_1_CD45_H12_S1056_comb !Sample_geo_accession = GSM3198497 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447029 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198498 !Sample_title = cy94_cd45pos_A11_S107_comb !Sample_geo_accession = GSM3198498 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447028 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198499 !Sample_title = cy84_Met_CD45_pos_D03_S903_comb !Sample_geo_accession = GSM3198499 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447027 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198500 !Sample_title = cy58_1_CD45_pos_H01_S661_comb !Sample_geo_accession = GSM3198500 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447026 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198501 !Sample_title = cy79_p5_CD45_pos_PD1_pos_A07_S583_comb !Sample_geo_accession = GSM3198501 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447025 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198502 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_G08_S272_comb !Sample_geo_accession = GSM3198502 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447024 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198503 !Sample_title = cy53_1_CD45_pos_1_D11_S47_comb !Sample_geo_accession = GSM3198503 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447023 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198504 !Sample_title = CY58_1_CD45_F05_S1025_comb !Sample_geo_accession = GSM3198504 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447022 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198505 !Sample_title = cy72_CD45_pos_A04_S868_comb !Sample_geo_accession = GSM3198505 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447021 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198506 !Sample_title = cy88_cd_45_pos_D11_S431_comb !Sample_geo_accession = GSM3198506 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447020 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198507 !Sample_title = cy74_CD45_pos_B05_S689_comb !Sample_geo_accession = GSM3198507 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447094 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198508 !Sample_title = CY58_1_CD45_H02_S1046_comb !Sample_geo_accession = GSM3198508 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447093 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198509 !Sample_title = cy94_cd45pos_4_B03_S15_comb !Sample_geo_accession = GSM3198509 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447092 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198510 !Sample_title = cy80_CD45_pos_PD1_pos_F07_S163_comb !Sample_geo_accession = GSM3198510 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447091 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198511 !Sample_title = cy80_Cd45_pos_PD1_pos_S191_H11_S191_comb !Sample_geo_accession = GSM3198511 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447005 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198512 !Sample_title = CY75_1FNA_G07_S559_comb !Sample_geo_accession = GSM3198512 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447004 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198513 !Sample_title = cy74_CD45_pos_C04_S700_comb !Sample_geo_accession = GSM3198513 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447003 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198514 !Sample_title = cy80_Cd45_pos_PD1_pos_S192_H12_S192_comb !Sample_geo_accession = GSM3198514 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447002 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198515 !Sample_title = cy60_1_cd_45_pos_3_A11_S299_comb !Sample_geo_accession = GSM3198515 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447001 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198516 !Sample_title = cy58_1_CD45_pos_B08_S596_comb !Sample_geo_accession = GSM3198516 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447000 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198517 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_E11_S539_comb !Sample_geo_accession = GSM3198517 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446999 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198518 !Sample_title = CY89A_CD45_POS_6_D03_S135_comb !Sample_geo_accession = GSM3198518 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446998 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198519 !Sample_title = cy94_cd45pos_F03_S159_comb !Sample_geo_accession = GSM3198519 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446997 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198520 !Sample_title = cy79_p5_CD45_pos_PD1_pos_G04_S652_comb !Sample_geo_accession = GSM3198520 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446996 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198521 !Sample_title = CY94CD45POS_1_H10_S190_comb !Sample_geo_accession = GSM3198521 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446993 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198522 !Sample_title = Cy74_CD45_G02_S458_comb !Sample_geo_accession = GSM3198522 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446992 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198523 !Sample_title = cy82_CD45_pos_3_G08_S80_comb !Sample_geo_accession = GSM3198523 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446991 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198524 !Sample_title = CY89FNA_D02_S230_comb !Sample_geo_accession = GSM3198524 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446990 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198525 !Sample_title = cy80_CD45_neg_F06_S450_comb !Sample_geo_accession = GSM3198525 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446995 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198526 !Sample_title = cy94_cd45pos_4_C09_S33_comb !Sample_geo_accession = GSM3198526 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446994 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198527 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_B02_S398_comb !Sample_geo_accession = GSM3198527 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446989 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198528 !Sample_title = CY94CD45POS_1_G11_S179_comb !Sample_geo_accession = GSM3198528 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446988 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198529 !Sample_title = Cy72_CD45_G12_S756_comb !Sample_geo_accession = GSM3198529 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446987 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198530 !Sample_title = cy80_CD45_pos_PD1_pos_E02_S146_comb !Sample_geo_accession = GSM3198530 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447056 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198531 !Sample_title = cy88_cd_45_pos_3_B07_S595_comb !Sample_geo_accession = GSM3198531 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447055 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198532 !Sample_title = cy88_cd_45_pos_3_B05_S593_comb !Sample_geo_accession = GSM3198532 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447054 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198533 !Sample_title = CY88CD45_150813_F01_S349_comb !Sample_geo_accession = GSM3198533 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447053 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198534 !Sample_title = cy80_Cd45_pos_Pd1_neg_S251_C11_S251_comb !Sample_geo_accession = GSM3198534 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447052 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198535 !Sample_title = cy79_p5_CD45_pos_PD1_pos_C11_S611_comb !Sample_geo_accession = GSM3198535 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447051 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198536 !Sample_title = cy72_CD45_pos_H09_S957_comb !Sample_geo_accession = GSM3198536 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447050 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198537 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_F04_S448_comb !Sample_geo_accession = GSM3198537 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447049 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198538 !Sample_title = cy80_Cd45_pos_PD1_pos_S162_G06_S162_comb !Sample_geo_accession = GSM3198538 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447048 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198539 !Sample_title = CY89A_Core_15_C10_S34_comb !Sample_geo_accession = GSM3198539 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447047 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198540 !Sample_title = cy79_p4_CD45_pos_PD1_pos_D02_S326_comb !Sample_geo_accession = GSM3198540 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447046 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198541 !Sample_title = cy79_p4_CD45_pos_PD1_pos_G08_S368_comb !Sample_geo_accession = GSM3198541 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447103 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198542 !Sample_title = cy94_cd45pos_4_D02_S38_comb !Sample_geo_accession = GSM3198542 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447102 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198543 !Sample_title = CY89COREQ1_H07_S379_comb !Sample_geo_accession = GSM3198543 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447101 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198544 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_C10_S226_comb !Sample_geo_accession = GSM3198544 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447100 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198545 !Sample_title = CY89A_CD45_POS_10_A06_S198_comb !Sample_geo_accession = GSM3198545 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447099 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198546 !Sample_title = cy74_CD45_pos_G02_S746_comb !Sample_geo_accession = GSM3198546 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447098 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198547 !Sample_title = cy94_cd45pos_4_G07_S79_comb !Sample_geo_accession = GSM3198547 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447097 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198548 !Sample_title = cy94_cd45pos_4_G11_S83_comb !Sample_geo_accession = GSM3198548 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447096 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198549 !Sample_title = cy79_p5_CD45_pos_PD1_pos_F08_S644_comb !Sample_geo_accession = GSM3198549 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447095 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198550 !Sample_title = CY84_PRIM_POS_ALL_6_A07_S391_comb !Sample_geo_accession = GSM3198550 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447168 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198551 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_H12_S1056_comb !Sample_geo_accession = GSM3198551 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447167 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198552 !Sample_title = CY75_1FNA_H02_S566_comb !Sample_geo_accession = GSM3198552 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447166 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198553 !Sample_title = CY75_1FNA_G12_S564_comb !Sample_geo_accession = GSM3198553 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447165 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198554 !Sample_title = cy79_p5_CD45_pos_PD1_pos_F12_S648_comb !Sample_geo_accession = GSM3198554 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447164 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198555 !Sample_title = Cy72_CD45_B02_S686_comb !Sample_geo_accession = GSM3198555 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447163 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198556 !Sample_title = CY58_1_CD45_B09_S981_comb !Sample_geo_accession = GSM3198556 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447162 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198557 !Sample_title = cy80_Cd45_pos_PD1_pos_D02_S86_comb !Sample_geo_accession = GSM3198557 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447161 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198558 !Sample_title = CY89A_CD45_POS_6_F10_S166_comb !Sample_geo_accession = GSM3198558 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447160 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198559 !Sample_title = cy60_1_cd_45_pos_3_B11_S311_comb !Sample_geo_accession = GSM3198559 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447159 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198560 !Sample_title = CY89A_Core_15_B06_S18_comb !Sample_geo_accession = GSM3198560 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447158 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198561 !Sample_title = cy84_Primary_CD45_pos_C06_S414_comb !Sample_geo_accession = GSM3198561 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447157 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198562 !Sample_title = CY89A_CD45_POS_6_G10_S178_comb !Sample_geo_accession = GSM3198562 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447156 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198563 !Sample_title = cy80_Cd45_pos_PD1_pos_S157_G01_S157_comb !Sample_geo_accession = GSM3198563 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447155 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198564 !Sample_title = cy60_1_cd_45_pos_3_C05_S317_comb !Sample_geo_accession = GSM3198564 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447154 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198565 !Sample_title = cy84_Met_CD45_pos_B05_S881_comb !Sample_geo_accession = GSM3198565 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447153 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198566 !Sample_title = Cy74_CD45_E04_S436_comb !Sample_geo_accession = GSM3198566 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447152 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198567 !Sample_title = CY88CD45_150813_B03_S303_comb !Sample_geo_accession = GSM3198567 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447151 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198568 !Sample_title = cy80_CD45_neg_B06_S402_comb !Sample_geo_accession = GSM3198568 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447150 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198569 !Sample_title = CY94CD45POS_1_C07_S127_comb !Sample_geo_accession = GSM3198569 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447149 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198570 !Sample_title = cy79_p4_CD45_pos_PD1_pos_A10_S298_comb !Sample_geo_accession = GSM3198570 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447148 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198571 !Sample_title = Cy81_FNA_CD45_A02_S194_comb !Sample_geo_accession = GSM3198571 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447059 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198572 !Sample_title = cy80_CD45_neg_G12_S468_comb !Sample_geo_accession = GSM3198572 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447058 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198573 !Sample_title = cy53_1_CD45_pos_1_D05_S41_comb !Sample_geo_accession = GSM3198573 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447057 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198574 !Sample_title = cy80_CD45_neg_A07_S391_comb !Sample_geo_accession = GSM3198574 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447130 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198575 !Sample_title = Cy81_FNA_CD45_H12_S288_comb !Sample_geo_accession = GSM3198575 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447129 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198576 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_G09_S273_comb !Sample_geo_accession = GSM3198576 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447128 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198577 !Sample_title = cy79_p5_CD45_pos_PD1_pos_H02_S662_comb !Sample_geo_accession = GSM3198577 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447127 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198578 !Sample_title = Cy81_FNA_CD45_A06_S198_comb !Sample_geo_accession = GSM3198578 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447126 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198579 !Sample_title = Cy74_CD45_F01_S445_comb !Sample_geo_accession = GSM3198579 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447125 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198580 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_H05_S281_comb !Sample_geo_accession = GSM3198580 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447124 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198581 !Sample_title = cy94_cd45pos_F07_S163_comb !Sample_geo_accession = GSM3198581 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447123 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198582 !Sample_title = cy79_p4_CD45_pos_PD1_pos_F06_S354_comb !Sample_geo_accession = GSM3198582 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447122 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198583 !Sample_title = Cy74_CD45_D01_S421_comb !Sample_geo_accession = GSM3198583 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447121 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198584 !Sample_title = Cy72_CD45_B07_S691_comb !Sample_geo_accession = GSM3198584 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447115 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198585 !Sample_title = Cy81_FNA_CD45_A03_S195_comb !Sample_geo_accession = GSM3198585 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447120 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198586 !Sample_title = Cy81_FNA_CD45_G02_S266_comb !Sample_geo_accession = GSM3198586 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447119 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198587 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_B06_S210_comb !Sample_geo_accession = GSM3198587 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447118 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198588 !Sample_title = cy80_Cd45_pos_Pd1_neg_S194_A02_S194_comb !Sample_geo_accession = GSM3198588 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447117 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198589 !Sample_title = Cy81_FNA_CD45_D11_S239_comb !Sample_geo_accession = GSM3198589 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447116 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198590 !Sample_title = cy80_Cd45_pos_PD1_pos_S161_G05_S161_comb !Sample_geo_accession = GSM3198590 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447114 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198591 !Sample_title = cy72_CD45_pos_E03_S915_comb !Sample_geo_accession = GSM3198591 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447113 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198592 !Sample_title = cy80_Cd45_pos_PD1_pos_B06_S42_comb !Sample_geo_accession = GSM3198592 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447112 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198593 !Sample_title = cy79_p5_CD45_pos_PD1_pos_E09_S633_comb !Sample_geo_accession = GSM3198593 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447111 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198594 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_C03_S507_comb !Sample_geo_accession = GSM3198594 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447110 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198595 !Sample_title = cy80_CD45_pos_PD1_pos_B12_S120_comb !Sample_geo_accession = GSM3198595 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447109 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198596 !Sample_title = cy80_Cd45_pos_PD1_pos_S182_H02_S182_comb !Sample_geo_accession = GSM3198596 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447108 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198597 !Sample_title = CY89A_Core_15_G05_S77_comb !Sample_geo_accession = GSM3198597 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447107 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198598 !Sample_title = CY89A_CD45_POS_6_C08_S128_comb !Sample_geo_accession = GSM3198598 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447106 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198599 !Sample_title = CY58_1_CD45_F10_S1030_comb !Sample_geo_accession = GSM3198599 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447105 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198600 !Sample_title = Cy81_FNA_CD45_C06_S222_comb !Sample_geo_accession = GSM3198600 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447104 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198601 !Sample_title = Cy72_CD45_B06_S690_comb !Sample_geo_accession = GSM3198601 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447147 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198602 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_D07_S235_comb !Sample_geo_accession = GSM3198602 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447146 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198603 !Sample_title = cy94_cd45pos_A06_S102_comb !Sample_geo_accession = GSM3198603 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447145 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198604 !Sample_title = cy53_1_CD45_pos_2_A09_S969_comb !Sample_geo_accession = GSM3198604 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447144 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198605 !Sample_title = cy80_CD45_neg_A05_S389_comb !Sample_geo_accession = GSM3198605 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447143 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198606 !Sample_title = cy80_CD45_neg_D03_S423_comb !Sample_geo_accession = GSM3198606 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447142 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198607 !Sample_title = cy94_cd45pos_4_D01_S37_comb !Sample_geo_accession = GSM3198607 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447141 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198608 !Sample_title = cy60_1_cd_45_pos_3_D01_S325_comb !Sample_geo_accession = GSM3198608 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447140 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198609 !Sample_title = cy82_CD45_pos_2_A07_S487_comb !Sample_geo_accession = GSM3198609 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447139 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198610 !Sample_title = CY89A_CD45_POS_6_G02_S170_comb !Sample_geo_accession = GSM3198610 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447138 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198611 !Sample_title = cy53_1_CD45_pos_1_F06_S66_comb !Sample_geo_accession = GSM3198611 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447137 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198612 !Sample_title = cy53_1_CD45_pos_1_G06_S78_comb !Sample_geo_accession = GSM3198612 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447136 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198613 !Sample_title = CY88CD45_150813_E01_S337_comb !Sample_geo_accession = GSM3198613 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447135 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198614 !Sample_title = cy53_1_CD45_pos_2_G05_S1037_comb !Sample_geo_accession = GSM3198614 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447134 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198615 !Sample_title = cy74_CD45_pos_E02_S722_comb !Sample_geo_accession = GSM3198615 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447133 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198616 !Sample_title = CY94CD45POS_1_D09_S141_comb !Sample_geo_accession = GSM3198616 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447132 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198617 !Sample_title = cy58_1_CD45_pos_F03_S639_comb !Sample_geo_accession = GSM3198617 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447131 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198618 !Sample_title = cy88_cd_45_pos_B07_S403_comb !Sample_geo_accession = GSM3198618 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447206 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198619 !Sample_title = cy88_cd45pos_5_F05_S257_comb !Sample_geo_accession = GSM3198619 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447205 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198620 !Sample_title = CY89A_Core_15_G06_S78_comb !Sample_geo_accession = GSM3198620 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447204 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198621 !Sample_title = CY88CD45POS_7_D05_S233_comb !Sample_geo_accession = GSM3198621 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447203 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198622 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_F09_S453_comb !Sample_geo_accession = GSM3198622 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447202 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198623 !Sample_title = cy94_cd45pos_4_H02_S86_comb !Sample_geo_accession = GSM3198623 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447201 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198624 !Sample_title = cy53_1_CD45_neg_D02_S326_comb !Sample_geo_accession = GSM3198624 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447200 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198625 !Sample_title = cy80_CD45_pos_PD1_pos_A06_S102_comb !Sample_geo_accession = GSM3198625 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447199 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198626 !Sample_title = cy84_Primary_CD45_pos_H02_S470_comb !Sample_geo_accession = GSM3198626 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447198 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198627 !Sample_title = CY88CD45_150813_E03_S339_comb !Sample_geo_accession = GSM3198627 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447197 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198628 !Sample_title = CY88CD45_150813_E06_S342_comb !Sample_geo_accession = GSM3198628 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447196 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198629 !Sample_title = cy58_1_CD45_pos_C09_S609_comb !Sample_geo_accession = GSM3198629 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447195 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198630 !Sample_title = cy60_1_cd_45_pos_3_A02_S290_comb !Sample_geo_accession = GSM3198630 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447194 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198631 !Sample_title = CY89A_CD45_POS_10_H07_S283_comb !Sample_geo_accession = GSM3198631 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446918 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198632 !Sample_title = cy80_Cd45_pos_PD1_pos_S137_F05_S137_comb !Sample_geo_accession = GSM3198632 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446917 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198633 !Sample_title = cy79_p4_CD45_pos_PD1_pos_E09_S345_comb !Sample_geo_accession = GSM3198633 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446916 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198634 !Sample_title = cy84_Primary_CD45_pos_B11_S407_comb !Sample_geo_accession = GSM3198634 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446915 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198635 !Sample_title = cy80_CD45_pos_PD1_pos_H03_S183_comb !Sample_geo_accession = GSM3198635 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446913 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198636 !Sample_title = cy80_Cd45_pos_Pd1_neg_S340_G04_S340_comb !Sample_geo_accession = GSM3198636 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446912 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198637 !Sample_title = cy79_p5_CD45_pos_PD1_pos_G05_S653_comb !Sample_geo_accession = GSM3198637 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446911 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198638 !Sample_title = cy88_cd_45_pos_C09_S417_comb !Sample_geo_accession = GSM3198638 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446986 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198639 !Sample_title = CY88CD45_150813_G02_S362_comb !Sample_geo_accession = GSM3198639 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446985 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198640 !Sample_title = Cy74_CD45_B03_S399_comb !Sample_geo_accession = GSM3198640 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446984 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198641 !Sample_title = Cy74_CD45_F08_S452_comb !Sample_geo_accession = GSM3198641 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446983 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198642 !Sample_title = cy80_Cd45_pos_Pd1_neg_S224_B08_S224_comb !Sample_geo_accession = GSM3198642 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446982 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198643 !Sample_title = cy79_p5_CD45_pos_PD1_pos_H07_S667_comb !Sample_geo_accession = GSM3198643 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446981 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198644 !Sample_title = CY89A_CD45_POS_6_B08_S116_comb !Sample_geo_accession = GSM3198644 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446980 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198645 !Sample_title = CY89A_CD45_POS_6_B02_S110_comb !Sample_geo_accession = GSM3198645 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446979 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198646 !Sample_title = Cy72_CD45_D01_S709_comb !Sample_geo_accession = GSM3198646 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446978 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198647 !Sample_title = CY89A_CD45_POS_6_G03_S171_comb !Sample_geo_accession = GSM3198647 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446977 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198648 !Sample_title = Cy74_CD45_G01_S457_comb !Sample_geo_accession = GSM3198648 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446976 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198649 !Sample_title = cy88_cd_45_pos_C07_S415_comb !Sample_geo_accession = GSM3198649 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446975 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198650 !Sample_title = Cy74_CD45_F02_S446_comb !Sample_geo_accession = GSM3198650 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446974 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198651 !Sample_title = Cy81_FNA_CD45_B08_S212_comb !Sample_geo_accession = GSM3198651 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446973 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198652 !Sample_title = Cy71_CD45_D12_S528_comb !Sample_geo_accession = GSM3198652 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446972 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198653 !Sample_title = cy79_p3_CD45_pos_PD1_neg_F09_S165_comb !Sample_geo_accession = GSM3198653 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446971 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198654 !Sample_title = cy74_CD45_pos_G11_S755_comb !Sample_geo_accession = GSM3198654 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446970 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198655 !Sample_title = cy72_CD45_pos_C07_S895_comb !Sample_geo_accession = GSM3198655 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446969 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198656 !Sample_title = cy80_Cd45_pos_PD1_pos_A09_S21_comb !Sample_geo_accession = GSM3198656 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446968 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198657 !Sample_title = cy88_cd_45_pos_E01_S433_comb !Sample_geo_accession = GSM3198657 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446967 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198658 !Sample_title = cy58_1_CD45_pos_G09_S657_comb !Sample_geo_accession = GSM3198658 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446966 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198659 !Sample_title = cy60_1_cd_45_pos_4_E09_S57_comb !Sample_geo_accession = GSM3198659 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446965 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198660 !Sample_title = CY94CD45POS_1_E12_S156_comb !Sample_geo_accession = GSM3198660 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446964 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198661 !Sample_title = cy79_p5_CD45_pos_PD1_pos_D03_S615_comb !Sample_geo_accession = GSM3198661 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447288 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198662 !Sample_title = cy58_1_CD45_pos_B07_S595_comb !Sample_geo_accession = GSM3198662 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446874 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198663 !Sample_title = cy58_1_CD45_pos_D03_S615_comb !Sample_geo_accession = GSM3198663 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446947 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198664 !Sample_title = cy72_CD45_pos_D10_S910_comb !Sample_geo_accession = GSM3198664 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446946 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198665 !Sample_title = cy82_CD45_pos_2_A05_S485_comb !Sample_geo_accession = GSM3198665 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446945 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198666 !Sample_title = cy60_1_cd_45_pos_3_G04_S364_comb !Sample_geo_accession = GSM3198666 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446944 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198667 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_G11_S275_comb !Sample_geo_accession = GSM3198667 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446943 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198668 !Sample_title = cy80_Cd45_pos_PD1_pos_S135_F03_S135_comb !Sample_geo_accession = GSM3198668 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446942 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198669 !Sample_title = cy60_1_cd_45_pos_4_C10_S34_comb !Sample_geo_accession = GSM3198669 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446941 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198670 !Sample_title = cy74_CD45_pos_C03_S699_comb !Sample_geo_accession = GSM3198670 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446940 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198671 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_A10_S202_comb !Sample_geo_accession = GSM3198671 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446939 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198672 !Sample_title = cy88_cd_45_pos_G02_S458_comb !Sample_geo_accession = GSM3198672 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446938 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198673 !Sample_title = cy58_1_CD45_pos_C11_S611_comb !Sample_geo_accession = GSM3198673 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446937 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198674 !Sample_title = cy60_1_cd_45_pos_4_E02_S50_comb !Sample_geo_accession = GSM3198674 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446936 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198675 !Sample_title = cy58_1_CD45_pos_G04_S652_comb !Sample_geo_accession = GSM3198675 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446935 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198676 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_C07_S223_comb !Sample_geo_accession = GSM3198676 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446934 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198677 !Sample_title = cy94_cd45pos_A10_S106_comb !Sample_geo_accession = GSM3198677 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446933 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198678 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_F10_S262_comb !Sample_geo_accession = GSM3198678 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446932 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198679 !Sample_title = cy88_cd_45_pos_H10_S478_comb !Sample_geo_accession = GSM3198679 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446931 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198680 !Sample_title = cy88_cd45pos_5_C03_S219_comb !Sample_geo_accession = GSM3198680 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446930 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198681 !Sample_title = CY89A_Core_15_C08_S32_comb !Sample_geo_accession = GSM3198681 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446929 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198682 !Sample_title = Cy74_CD45_H06_S474_comb !Sample_geo_accession = GSM3198682 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446928 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198683 !Sample_title = CY89A_Core_15_C09_S33_comb !Sample_geo_accession = GSM3198683 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446927 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198684 !Sample_title = cy80_Cd45_pos_PD1_pos_B01_S37_comb !Sample_geo_accession = GSM3198684 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446926 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198685 !Sample_title = Cy72_CD45_H06_S762_comb !Sample_geo_accession = GSM3198685 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446925 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198686 !Sample_title = cy80_CD45_neg_E04_S436_comb !Sample_geo_accession = GSM3198686 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446924 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198687 !Sample_title = cy58_1_CD45_pos_H08_S668_comb !Sample_geo_accession = GSM3198687 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446923 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198688 !Sample_title = cy94_cd45neg_cd90pos_D02_S326_comb !Sample_geo_accession = GSM3198688 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446922 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198689 !Sample_title = CY89A_CD45_POS_6_D02_S134_comb !Sample_geo_accession = GSM3198689 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446921 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198690 !Sample_title = CY88CD45POS_2_D03_S423_comb !Sample_geo_accession = GSM3198690 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446920 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198691 !Sample_title = cy72_CD45_pos_D07_S907_comb !Sample_geo_accession = GSM3198691 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446919 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198692 !Sample_title = CY88CD45_150813_G07_S367_comb !Sample_geo_accession = GSM3198692 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446904 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198693 !Sample_title = cy79_p5_CD45_pos_PD1_pos_C07_S607_comb !Sample_geo_accession = GSM3198693 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446903 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198694 !Sample_title = cy84_Primary_CD45_pos_E04_S436_comb !Sample_geo_accession = GSM3198694 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446902 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198695 !Sample_title = CY84_PRIM_POS_ALL_7_B11_S215_comb !Sample_geo_accession = GSM3198695 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446901 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198696 !Sample_title = cy74_CD45_pos_C07_S703_comb !Sample_geo_accession = GSM3198696 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446900 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198697 !Sample_title = cy80_Cd45_pos_PD1_pos_S140_F08_S140_comb !Sample_geo_accession = GSM3198697 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446899 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198698 !Sample_title = cy60_1_cd_45_pos_3_H04_S376_comb !Sample_geo_accession = GSM3198698 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446898 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198699 !Sample_title = cy79_p4_CD45_pos_PD1_pos_E07_S343_comb !Sample_geo_accession = GSM3198699 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446897 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198700 !Sample_title = cy82_CD45_pos_1_F08_S548_comb !Sample_geo_accession = GSM3198700 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446896 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198701 !Sample_title = CY75_1FNA_F01_S541_comb !Sample_geo_accession = GSM3198701 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446895 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198702 !Sample_title = cy80_CD45_neg_H03_S471_comb !Sample_geo_accession = GSM3198702 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446892 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198703 !Sample_title = cy80_CD45_neg_G01_S457_comb !Sample_geo_accession = GSM3198703 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446891 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198704 !Sample_title = CY89A_Core_15_H12_S96_comb !Sample_geo_accession = GSM3198704 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446890 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198705 !Sample_title = cy88_cd_45_pos_F07_S451_comb !Sample_geo_accession = GSM3198705 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446894 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198706 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_B08_S404_comb !Sample_geo_accession = GSM3198706 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446893 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198707 !Sample_title = CY94CD45POS_1_B09_S117_comb !Sample_geo_accession = GSM3198707 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446889 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198708 !Sample_title = CY89A_CD45_POS_6_D10_S142_comb !Sample_geo_accession = GSM3198708 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446888 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198709 !Sample_title = cy88_cd_45_pos_H07_S475_comb !Sample_geo_accession = GSM3198709 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446887 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198710 !Sample_title = Cy72_CD45_G01_S745_comb !Sample_geo_accession = GSM3198710 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446886 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198711 !Sample_title = cy79_p4_CD45_pos_PD1_pos_F01_S349_comb !Sample_geo_accession = GSM3198711 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446885 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198712 !Sample_title = cy84_Primary_CD45_pos_D10_S430_comb !Sample_geo_accession = GSM3198712 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446884 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198713 !Sample_title = cy53_1_CD45_pos_2_H08_S1052_comb !Sample_geo_accession = GSM3198713 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446883 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198714 !Sample_title = cy80_CD45_neg_C12_S420_comb !Sample_geo_accession = GSM3198714 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446882 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198715 !Sample_title = cy80_Cd45_pos_PD1_pos_B07_S43_comb !Sample_geo_accession = GSM3198715 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446881 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198716 !Sample_title = Cy74_CD45_H12_S480_comb !Sample_geo_accession = GSM3198716 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446880 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198717 !Sample_title = cy79_p4_CD45_pos_PD1_pos_C10_S322_comb !Sample_geo_accession = GSM3198717 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446879 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198718 !Sample_title = CY58_1_CD45_G11_S1043_comb !Sample_geo_accession = GSM3198718 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446878 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198719 !Sample_title = cy94_cd45neg_cd90pos_C03_S315_comb !Sample_geo_accession = GSM3198719 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446877 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198720 !Sample_title = cy58_1_CD45_pos_E08_S632_comb !Sample_geo_accession = GSM3198720 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446876 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198721 !Sample_title = CY89COREQ1_B04_S304_comb !Sample_geo_accession = GSM3198721 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446875 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198722 !Sample_title = cy80_CD45_neg_A12_S396_comb !Sample_geo_accession = GSM3198722 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446862 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198723 !Sample_title = CY89FNA_A02_S194_comb !Sample_geo_accession = GSM3198723 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446861 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198724 !Sample_title = CY58_1_CD45_D04_S1000_comb !Sample_geo_accession = GSM3198724 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446860 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198725 !Sample_title = Cy72_CD45_A11_S683_comb !Sample_geo_accession = GSM3198725 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446859 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198726 !Sample_title = CY89A_Core_15_B05_S17_comb !Sample_geo_accession = GSM3198726 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446858 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198727 !Sample_title = CY94CD45POS_1_H01_S181_comb !Sample_geo_accession = GSM3198727 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446857 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198728 !Sample_title = Cy81_FNA_CD45_C11_S227_comb !Sample_geo_accession = GSM3198728 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446856 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198729 !Sample_title = cy80_Cd45_pos_Pd1_neg_S265_D01_S265_comb !Sample_geo_accession = GSM3198729 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446855 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198730 !Sample_title = cy94_cd45pos_D05_S137_comb !Sample_geo_accession = GSM3198730 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446854 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198731 !Sample_title = Cy71_CD45_F03_S543_comb !Sample_geo_accession = GSM3198731 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446853 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198732 !Sample_title = CY89COREQ1_C03_S315_comb !Sample_geo_accession = GSM3198732 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446852 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198733 !Sample_title = cy72_CD45_pos_A05_S869_comb !Sample_geo_accession = GSM3198733 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446851 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198734 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_F12_S456_comb !Sample_geo_accession = GSM3198734 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446850 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198735 !Sample_title = CY88CD45POS_2_D11_S431_comb !Sample_geo_accession = GSM3198735 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446849 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198736 !Sample_title = Cy72_CD45_E10_S730_comb !Sample_geo_accession = GSM3198736 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446848 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198737 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_B09_S213_comb !Sample_geo_accession = GSM3198737 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446847 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198738 !Sample_title = cy60_1_cd_45_pos_4_B11_S23_comb !Sample_geo_accession = GSM3198738 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446846 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198739 !Sample_title = CY58_1_CD45_F09_S1029_comb !Sample_geo_accession = GSM3198739 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446845 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198740 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_B12_S408_comb !Sample_geo_accession = GSM3198740 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446844 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198741 !Sample_title = cy60_1_cd_45_pos_3_C07_S319_comb !Sample_geo_accession = GSM3198741 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446843 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198742 !Sample_title = cy79_p5_CD45_pos_PD1_pos_G12_S660_comb !Sample_geo_accession = GSM3198742 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446842 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198743 !Sample_title = CY94CD45POS_1_A09_S105_comb !Sample_geo_accession = GSM3198743 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446841 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198744 !Sample_title = cy79_p5_CD45_pos_PD1_pos_E04_S628_comb !Sample_geo_accession = GSM3198744 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446840 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198745 !Sample_title = cy72_CD45_pos_E04_S916_comb !Sample_geo_accession = GSM3198745 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446839 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198746 !Sample_title = cy60_1_cd_45_pos_3_H12_S384_comb !Sample_geo_accession = GSM3198746 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446910 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198747 !Sample_title = cy79_p5_CD45_pos_PD1_pos_B08_S596_comb !Sample_geo_accession = GSM3198747 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446909 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198748 !Sample_title = CY89COREQ1_D01_S325_comb !Sample_geo_accession = GSM3198748 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446908 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198749 !Sample_title = cy80_CD45_neg_F04_S448_comb !Sample_geo_accession = GSM3198749 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446907 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198750 !Sample_title = CY58_1_CD45_E05_S1013_comb !Sample_geo_accession = GSM3198750 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446906 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198751 !Sample_title = Cy71_CD45_C01_S505_comb !Sample_geo_accession = GSM3198751 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446905 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198752 !Sample_title = cy80_Cd45_pos_Pd1_neg_S364_H04_S364_comb !Sample_geo_accession = GSM3198752 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446963 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198753 !Sample_title = CY58_1_CD45_F04_S1024_comb !Sample_geo_accession = GSM3198753 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446962 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198754 !Sample_title = cy94_cd45pos_B11_S119_comb !Sample_geo_accession = GSM3198754 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446961 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198755 !Sample_title = cy79_p4_CD45_pos_PD1_pos_F04_S352_comb !Sample_geo_accession = GSM3198755 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446960 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198756 !Sample_title = cy74_CD45_pos_E03_S723_comb !Sample_geo_accession = GSM3198756 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446959 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198757 !Sample_title = cy53_1_CD45_pos_2_G02_S1034_comb !Sample_geo_accession = GSM3198757 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446958 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198758 !Sample_title = CY58_1_CD45_F12_S1032_comb !Sample_geo_accession = GSM3198758 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446957 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198759 !Sample_title = CY89COREQ1_D03_S327_comb !Sample_geo_accession = GSM3198759 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446956 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198760 !Sample_title = cy80_Cd45_pos_PD1_pos_S190_H10_S190_comb !Sample_geo_accession = GSM3198760 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446955 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198761 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_H12_S288_comb !Sample_geo_accession = GSM3198761 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446954 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198762 !Sample_title = cy79_p5_CD45_pos_PD1_pos_H06_S666_comb !Sample_geo_accession = GSM3198762 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446953 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198763 !Sample_title = cy74_CD45_pos_A05_S677_comb !Sample_geo_accession = GSM3198763 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446948 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198764 !Sample_title = cy79_p5_CD45_pos_PD1_pos_F02_S638_comb !Sample_geo_accession = GSM3198764 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447019 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198765 !Sample_title = CY58_1_CD45_G09_S1041_comb !Sample_geo_accession = GSM3198765 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446952 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198766 !Sample_title = cy84_Primary_CD45_pos_B07_S403_comb !Sample_geo_accession = GSM3198766 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446951 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198767 !Sample_title = cy94_cd45pos_4_A02_S2_comb !Sample_geo_accession = GSM3198767 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446950 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198768 !Sample_title = cy58_1_CD45_pos_F10_S646_comb !Sample_geo_accession = GSM3198768 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446949 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198769 !Sample_title = Cy81_FNA_CD45_F04_S256_comb !Sample_geo_accession = GSM3198769 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447018 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198770 !Sample_title = cy94_cd45pos_D08_S140_comb !Sample_geo_accession = GSM3198770 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447017 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198771 !Sample_title = cy79_p4_CD45_pos_PD1_pos_G05_S365_comb !Sample_geo_accession = GSM3198771 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447016 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198772 !Sample_title = cy84_Primary_CD45_pos_D11_S431_comb !Sample_geo_accession = GSM3198772 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447015 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198773 !Sample_title = CY94CD45POS_1_G04_S172_comb !Sample_geo_accession = GSM3198773 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447014 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198774 !Sample_title = cy72_CD45_pos_C06_S894_comb !Sample_geo_accession = GSM3198774 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447013 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198775 !Sample_title = cy58_1_CD45_pos_H09_S669_comb !Sample_geo_accession = GSM3198775 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447012 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198776 !Sample_title = cy60_1_cd_45_pos_4_D10_S46_comb !Sample_geo_accession = GSM3198776 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447011 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198777 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_G04_S460_comb !Sample_geo_accession = GSM3198777 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447010 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198778 !Sample_title = Cy74_CD45_G04_S460_comb !Sample_geo_accession = GSM3198778 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447009 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198779 !Sample_title = cy72_CD45_pos_D08_S908_comb !Sample_geo_accession = GSM3198779 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447008 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198780 !Sample_title = cy94_cd45pos_4_C11_S35_comb !Sample_geo_accession = GSM3198780 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447007 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198781 !Sample_title = CY89A_Core_15_A01_S1_comb !Sample_geo_accession = GSM3198781 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447006 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198782 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_B01_S397_comb !Sample_geo_accession = GSM3198782 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446732 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198783 !Sample_title = cy88_cd_45_pos_H11_S479_comb !Sample_geo_accession = GSM3198783 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446731 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198784 !Sample_title = CY89COREQ1_E02_S338_comb !Sample_geo_accession = GSM3198784 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446730 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198785 !Sample_title = CY58_1_CD45_C03_S987_comb !Sample_geo_accession = GSM3198785 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446729 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198786 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_E01_S433_comb !Sample_geo_accession = GSM3198786 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446728 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198787 !Sample_title = CY89A_Core_15_G07_S79_comb !Sample_geo_accession = GSM3198787 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446727 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198788 !Sample_title = CY94CD45POS_1_H02_S182_comb !Sample_geo_accession = GSM3198788 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446726 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198789 !Sample_title = cy80_Cd45_pos_Pd1_neg_S337_G01_S337_comb !Sample_geo_accession = GSM3198789 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446725 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198790 !Sample_title = CY88CD45POS_7_F01_S253_comb !Sample_geo_accession = GSM3198790 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446724 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198791 !Sample_title = cy60_1_cd_45_pos_3_B06_S306_comb !Sample_geo_accession = GSM3198791 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446723 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198792 !Sample_title = cy60_1_cd_45_pos_3_F08_S356_comb !Sample_geo_accession = GSM3198792 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446722 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198793 !Sample_title = cy79_p4_CD45_pos_PD1_pos_G02_S362_comb !Sample_geo_accession = GSM3198793 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446721 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198794 !Sample_title = CY89FNA_G10_S274_comb !Sample_geo_accession = GSM3198794 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446720 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198795 !Sample_title = CY88CD45POS_7_H05_S281_comb !Sample_geo_accession = GSM3198795 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446719 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198796 !Sample_title = Cy72_CD45_F05_S737_comb !Sample_geo_accession = GSM3198796 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446718 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198797 !Sample_title = cy84_Primary_CD45_pos_B09_S405_comb !Sample_geo_accession = GSM3198797 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446717 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198798 !Sample_title = CY89A_CD45_POS_6_D04_S136_comb !Sample_geo_accession = GSM3198798 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446716 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198799 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_B03_S399_comb !Sample_geo_accession = GSM3198799 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446715 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198800 !Sample_title = CY88CD45POS_7_G05_S269_comb !Sample_geo_accession = GSM3198800 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446714 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198801 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_B08_S212_comb !Sample_geo_accession = GSM3198801 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446713 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198802 !Sample_title = cy60_1_cd_45_pos_3_C03_S315_comb !Sample_geo_accession = GSM3198802 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446712 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198803 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_B02_S206_comb !Sample_geo_accession = GSM3198803 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446779 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198804 !Sample_title = Cy74_CD45_D03_S423_comb !Sample_geo_accession = GSM3198804 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446778 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198805 !Sample_title = cy72_CD45_pos_B02_S878_comb !Sample_geo_accession = GSM3198805 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446776 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198806 !Sample_title = cy53_1_CD45_pos_2_B01_S973_comb !Sample_geo_accession = GSM3198806 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446775 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198807 !Sample_title = cy94_cd45pos_4_C01_S25_comb !Sample_geo_accession = GSM3198807 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446774 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198808 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_C10_S418_comb !Sample_geo_accession = GSM3198808 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446773 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198809 !Sample_title = cy53_1_CD45_pos_2_B05_S977_comb !Sample_geo_accession = GSM3198809 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446772 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198810 !Sample_title = cy80_Cd45_pos_PD1_pos_D11_S95_comb !Sample_geo_accession = GSM3198810 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446771 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198811 !Sample_title = cy53_1_CD45_pos_1_D10_S46_comb !Sample_geo_accession = GSM3198811 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446770 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198812 !Sample_title = Cy74_CD45_G03_S459_comb !Sample_geo_accession = GSM3198812 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446683 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198813 !Sample_title = CY89COREQ1_D10_S334_comb !Sample_geo_accession = GSM3198813 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446749 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198814 !Sample_title = cy80_Cd45_pos_PD1_pos_A06_S18_comb !Sample_geo_accession = GSM3198814 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446748 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198815 !Sample_title = Cy81_FNA_CD45_A07_S199_comb !Sample_geo_accession = GSM3198815 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446747 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198816 !Sample_title = CY89FNA_C08_S224_comb !Sample_geo_accession = GSM3198816 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446746 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198817 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_D02_S422_comb !Sample_geo_accession = GSM3198817 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446745 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198818 !Sample_title = CY88CD45_150813_G05_S365_comb !Sample_geo_accession = GSM3198818 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446744 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198819 !Sample_title = cy53_1_CD45_pos_2_F07_S1027_comb !Sample_geo_accession = GSM3198819 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446743 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198820 !Sample_title = cy94_cd45pos_4_H09_S93_comb !Sample_geo_accession = GSM3198820 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446742 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198821 !Sample_title = cy53_1_CD45_pos_2_D04_S1000_comb !Sample_geo_accession = GSM3198821 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446741 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198822 !Sample_title = CY89A_CD45_POS_10_G06_S270_comb !Sample_geo_accession = GSM3198822 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446740 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198823 !Sample_title = cy79_p4_CD45_pos_PD1_pos_F02_S350_comb !Sample_geo_accession = GSM3198823 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446739 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198824 !Sample_title = cy80_Cd45_pos_Pd1_neg_S243_C03_S243_comb !Sample_geo_accession = GSM3198824 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446738 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198825 !Sample_title = cy74_CD45_pos_G07_S751_comb !Sample_geo_accession = GSM3198825 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446737 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198826 !Sample_title = cy82_CD45_pos_1_H09_S573_comb !Sample_geo_accession = GSM3198826 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446736 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198827 !Sample_title = cy53_1_CD45_pos_1_C09_S33_comb !Sample_geo_accession = GSM3198827 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446735 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198828 !Sample_title = cy80_Cd45_pos_PD1_pos_A07_S19_comb !Sample_geo_accession = GSM3198828 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446734 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198829 !Sample_title = Cy72_CD45_B05_S689_comb !Sample_geo_accession = GSM3198829 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446733 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198830 !Sample_title = cy88_cd_45_pos_H06_S474_comb !Sample_geo_accession = GSM3198830 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446769 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198831 !Sample_title = cy53_1_CD45_pos_2_A02_S962_comb !Sample_geo_accession = GSM3198831 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446768 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198832 !Sample_title = cy74_CD45_pos_F03_S735_comb !Sample_geo_accession = GSM3198832 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446767 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198833 !Sample_title = cy84_Met_CD45_pos_B02_S878_comb !Sample_geo_accession = GSM3198833 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446766 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198834 !Sample_title = CY58_1_CD45_A07_S967_comb !Sample_geo_accession = GSM3198834 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446765 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198835 !Sample_title = cy80_CD45_pos_PD1_pos_C03_S123_comb !Sample_geo_accession = GSM3198835 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446764 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198836 !Sample_title = CY89A_Core_15_G01_S73_comb !Sample_geo_accession = GSM3198836 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446763 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198837 !Sample_title = CY88CD45POS_7_G07_S271_comb !Sample_geo_accession = GSM3198837 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446762 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198838 !Sample_title = cy78_CD45_neg_2_A06_S582_comb !Sample_geo_accession = GSM3198838 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446761 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198839 !Sample_title = cy80_Cd45_pos_Pd1_neg_S226_B10_S226_comb !Sample_geo_accession = GSM3198839 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446760 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198840 !Sample_title = cy80_CD45_pos_PD1_pos_F04_S160_comb !Sample_geo_accession = GSM3198840 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446759 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198841 !Sample_title = cy80_Cd45_pos_PD1_pos_S166_G10_S166_comb !Sample_geo_accession = GSM3198841 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446758 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198842 !Sample_title = cy80_CD45_neg_A11_S395_comb !Sample_geo_accession = GSM3198842 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446757 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198843 !Sample_title = CY58_1_CD45_F03_S1023_comb !Sample_geo_accession = GSM3198843 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446756 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198844 !Sample_title = CY94CD45POS_1_A02_S98_comb !Sample_geo_accession = GSM3198844 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446755 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198845 !Sample_title = cy53_1_CD45_pos_1_D04_S40_comb !Sample_geo_accession = GSM3198845 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446754 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198846 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_F09_S261_comb !Sample_geo_accession = GSM3198846 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446753 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198847 !Sample_title = CY89FNA_D07_S235_comb !Sample_geo_accession = GSM3198847 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446752 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198848 !Sample_title = cy80_CD45_pos_PD1_pos_D02_S134_comb !Sample_geo_accession = GSM3198848 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446751 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198849 !Sample_title = CY89COREQ1_D11_S335_comb !Sample_geo_accession = GSM3198849 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446750 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198850 !Sample_title = cy80_Cd45_pos_Pd1_neg_S225_B09_S225_comb !Sample_geo_accession = GSM3198850 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446807 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198851 !Sample_title = cy74_CD45_pos_F01_S733_comb !Sample_geo_accession = GSM3198851 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446806 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198852 !Sample_title = cy79_p5_CD45_pos_PD1_pos_D04_S616_comb !Sample_geo_accession = GSM3198852 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446805 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198853 !Sample_title = CY89A_CD45_POS_10_A10_S202_comb !Sample_geo_accession = GSM3198853 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446804 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198854 !Sample_title = cy80_CD45_pos_PD1_pos_C01_S121_comb !Sample_geo_accession = GSM3198854 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446826 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198855 !Sample_title = cy82_CD45_pos_2_A12_S492_comb !Sample_geo_accession = GSM3198855 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446803 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198856 !Sample_title = cy79_p5_CD45_pos_PD1_pos_G08_S656_comb !Sample_geo_accession = GSM3198856 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446802 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198857 !Sample_title = CY89FNA_G02_S266_comb !Sample_geo_accession = GSM3198857 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446801 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198858 !Sample_title = cy80_Cd45_pos_PD1_pos_S144_F12_S144_comb !Sample_geo_accession = GSM3198858 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446800 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198859 !Sample_title = cy60_1_cd_45_pos_3_E02_S338_comb !Sample_geo_accession = GSM3198859 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446799 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198860 !Sample_title = Cy74_CD45_G08_S464_comb !Sample_geo_accession = GSM3198860 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446798 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198861 !Sample_title = cy79_p4_CD45_pos_PD1_pos_D06_S330_comb !Sample_geo_accession = GSM3198861 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446825 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198862 !Sample_title = cy72_CD45_pos_B04_S880_comb !Sample_geo_accession = GSM3198862 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446824 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198863 !Sample_title = cy79_p5_CD45_pos_PD1_pos_E10_S634_comb !Sample_geo_accession = GSM3198863 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446823 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198864 !Sample_title = cy88_cd45pos_5_G02_S266_comb !Sample_geo_accession = GSM3198864 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446822 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198865 !Sample_title = cy74_CD45_pos_C11_S707_comb !Sample_geo_accession = GSM3198865 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446821 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198866 !Sample_title = cy84_Met_CD45_pos_B07_S883_comb !Sample_geo_accession = GSM3198866 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446820 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198867 !Sample_title = CY89A_Core_15_F12_S72_comb !Sample_geo_accession = GSM3198867 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446819 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198868 !Sample_title = cy94_cd45pos_D06_S138_comb !Sample_geo_accession = GSM3198868 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446818 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198869 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_D08_S236_comb !Sample_geo_accession = GSM3198869 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446817 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198870 !Sample_title = Cy72_CD45_F02_S734_comb !Sample_geo_accession = GSM3198870 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446816 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198871 !Sample_title = Cy74_CD45_E08_S440_comb !Sample_geo_accession = GSM3198871 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446815 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198872 !Sample_title = Cy81_FNA_CD45_C02_S218_comb !Sample_geo_accession = GSM3198872 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446814 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198873 !Sample_title = CY58_1_CD45_A04_S964_comb !Sample_geo_accession = GSM3198873 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446813 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198874 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_F05_S257_comb !Sample_geo_accession = GSM3198874 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446812 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198875 !Sample_title = cy82_CD45_pos_2_B02_S494_comb !Sample_geo_accession = GSM3198875 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446811 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198876 !Sample_title = cy53_1_CD45_pos_1_B07_S19_comb !Sample_geo_accession = GSM3198876 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446810 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198877 !Sample_title = cy94_cd45pos_G10_S178_comb !Sample_geo_accession = GSM3198877 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446809 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198878 !Sample_title = cy94_cd45pos_A01_S97_comb !Sample_geo_accession = GSM3198878 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446808 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198879 !Sample_title = cy74_CD45_pos_E09_S729_comb !Sample_geo_accession = GSM3198879 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446873 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198880 !Sample_title = cy60_1_cd_45_pos_4_D06_S42_comb !Sample_geo_accession = GSM3198880 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446872 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198881 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_F03_S447_comb !Sample_geo_accession = GSM3198881 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446871 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198882 !Sample_title = cy84_Primary_CD45_pos_B10_S406_comb !Sample_geo_accession = GSM3198882 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446870 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198883 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_H01_S1045_comb !Sample_geo_accession = GSM3198883 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446869 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198884 !Sample_title = CY88CD45POS_2_C05_S413_comb !Sample_geo_accession = GSM3198884 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446863 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198885 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_F10_S454_comb !Sample_geo_accession = GSM3198885 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446868 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198886 !Sample_title = CY75_1FNA_E11_S539_comb !Sample_geo_accession = GSM3198886 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446867 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198887 !Sample_title = CY88CD45_150813_G09_S369_comb !Sample_geo_accession = GSM3198887 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446866 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198888 !Sample_title = CY88CD45_150813_E11_S347_comb !Sample_geo_accession = GSM3198888 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446865 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198889 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_H07_S283_comb !Sample_geo_accession = GSM3198889 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446864 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198890 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_F03_S255_comb !Sample_geo_accession = GSM3198890 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446797 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198891 !Sample_title = cy72_CD45_pos_F09_S933_comb !Sample_geo_accession = GSM3198891 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446796 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198892 !Sample_title = cy53_1_CD45_pos_2_G01_S1033_comb !Sample_geo_accession = GSM3198892 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446795 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198893 !Sample_title = Cy74_CD45_G07_S463_comb !Sample_geo_accession = GSM3198893 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446794 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198894 !Sample_title = cy79_p5_CD45_pos_PD1_pos_E01_S625_comb !Sample_geo_accession = GSM3198894 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446793 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198895 !Sample_title = cy60_1_cd_45_pos_4_D07_S43_comb !Sample_geo_accession = GSM3198895 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446792 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198896 !Sample_title = cy58_1_CD45_pos_B05_S593_comb !Sample_geo_accession = GSM3198896 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446791 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198897 !Sample_title = cy58_1_CD45_pos_A10_S586_comb !Sample_geo_accession = GSM3198897 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446790 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198898 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_H10_S574_comb !Sample_geo_accession = GSM3198898 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446789 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198899 !Sample_title = CY94CD45POS_1_F02_S158_comb !Sample_geo_accession = GSM3198899 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446788 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198900 !Sample_title = CY89FNA_A01_S193_comb !Sample_geo_accession = GSM3198900 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446787 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198901 !Sample_title = CY89A_Core_15_G11_S83_comb !Sample_geo_accession = GSM3198901 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446786 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198902 !Sample_title = CY75_1FNA_H08_S572_comb !Sample_geo_accession = GSM3198902 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446785 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198903 !Sample_title = cy94_cd45pos_4_H04_S88_comb !Sample_geo_accession = GSM3198903 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446784 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198904 !Sample_title = cy80_CD45_neg_H07_S475_comb !Sample_geo_accession = GSM3198904 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446783 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198905 !Sample_title = Cy81_FNA_CD45_A05_S197_comb !Sample_geo_accession = GSM3198905 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446782 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198906 !Sample_title = cy80_Cd45_pos_PD1_pos_S142_F10_S142_comb !Sample_geo_accession = GSM3198906 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446781 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198907 !Sample_title = cy74_CD45_pos_A07_S679_comb !Sample_geo_accession = GSM3198907 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446780 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198908 !Sample_title = cy80_CD45_pos_PD1_pos_F01_S157_comb !Sample_geo_accession = GSM3198908 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446838 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198909 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_E07_S1015_comb !Sample_geo_accession = GSM3198909 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446837 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198910 !Sample_title = Cy81_FNA_CD45_A08_S200_comb !Sample_geo_accession = GSM3198910 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446836 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198911 !Sample_title = CY88CD45POS_7_F07_S259_comb !Sample_geo_accession = GSM3198911 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446835 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198912 !Sample_title = cy80_CD45_pos_PD1_pos_H07_S187_comb !Sample_geo_accession = GSM3198912 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446831 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198913 !Sample_title = cy79_p4_CD45_pos_PD1_pos_G03_S363_comb !Sample_geo_accession = GSM3198913 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446830 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198914 !Sample_title = cy94_cd45pos_G12_S180_comb !Sample_geo_accession = GSM3198914 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446829 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198915 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_E05_S437_comb !Sample_geo_accession = GSM3198915 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446834 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198916 !Sample_title = cy80_CD45_pos_PD1_pos_C05_S125_comb !Sample_geo_accession = GSM3198916 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446833 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198917 !Sample_title = cy94_cd45pos_G08_S176_comb !Sample_geo_accession = GSM3198917 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446832 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198918 !Sample_title = CY94CD45POS_1_C12_S132_comb !Sample_geo_accession = GSM3198918 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446828 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198919 !Sample_title = cy53_1_CD45_pos_1_A11_S11_comb !Sample_geo_accession = GSM3198919 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446827 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198920 !Sample_title = Cy74_CD45_B06_S402_comb !Sample_geo_accession = GSM3198920 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446647 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198921 !Sample_title = cy79_p5_CD45_pos_PD1_pos_F03_S639_comb !Sample_geo_accession = GSM3198921 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446646 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198922 !Sample_title = cy60_1_cd_45_pos_4_G07_S79_comb !Sample_geo_accession = GSM3198922 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446645 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198923 !Sample_title = Cy74_CD45_H02_S470_comb !Sample_geo_accession = GSM3198923 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446644 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198924 !Sample_title = cy79_p4_CD45_pos_PD1_pos_E01_S337_comb !Sample_geo_accession = GSM3198924 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446643 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198925 !Sample_title = CY58_1_CD45_B08_S980_comb !Sample_geo_accession = GSM3198925 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446642 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198926 !Sample_title = cy58_1_CD45_pos_C06_S606_comb !Sample_geo_accession = GSM3198926 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446641 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198927 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_E08_S440_comb !Sample_geo_accession = GSM3198927 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446640 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198928 !Sample_title = CY89COREQ1_A08_S296_comb !Sample_geo_accession = GSM3198928 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446639 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198929 !Sample_title = cy79_p4_CD45_pos_PD1_pos_F03_S351_comb !Sample_geo_accession = GSM3198929 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446638 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198930 !Sample_title = Cy72_CD45_F07_S739_comb !Sample_geo_accession = GSM3198930 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446637 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198931 !Sample_title = cy88_cd_45_pos_3_F09_S645_comb !Sample_geo_accession = GSM3198931 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446636 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198932 !Sample_title = cy72_CD45_pos_F04_S928_comb !Sample_geo_accession = GSM3198932 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446635 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198933 !Sample_title = Cy71_CD45_C08_S512_comb !Sample_geo_accession = GSM3198933 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446634 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198934 !Sample_title = cy80_CD45_pos_PD1_pos_C12_S132_comb !Sample_geo_accession = GSM3198934 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446633 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198935 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_E07_S439_comb !Sample_geo_accession = GSM3198935 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446632 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198936 !Sample_title = cy58_1_CD45_pos_D01_S613_comb !Sample_geo_accession = GSM3198936 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446631 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198937 !Sample_title = CY75_1FNA_E12_S540_comb !Sample_geo_accession = GSM3198937 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446630 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198938 !Sample_title = cy53_1_CD45_pos_2_F04_S1024_comb !Sample_geo_accession = GSM3198938 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446629 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198939 !Sample_title = CY94CD45POS_1_G07_S175_comb !Sample_geo_accession = GSM3198939 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446628 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198940 !Sample_title = cy79_p4_CD45_pos_PD1_pos_G12_S372_comb !Sample_geo_accession = GSM3198940 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446627 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198941 !Sample_title = cy58_1_CD45_pos_A02_S578_comb !Sample_geo_accession = GSM3198941 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446626 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198942 !Sample_title = CY88CD45POS_7_E01_S241_comb !Sample_geo_accession = GSM3198942 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446625 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198943 !Sample_title = cy80_Cd45_pos_PD1_pos_S110_E02_S110_comb !Sample_geo_accession = GSM3198943 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446624 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198944 !Sample_title = cy94_cd45pos_G02_S170_comb !Sample_geo_accession = GSM3198944 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446623 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198945 !Sample_title = cy53_1_CD45_pos_2_E07_S1015_comb !Sample_geo_accession = GSM3198945 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446622 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198946 !Sample_title = Cy74_CD45_C06_S414_comb !Sample_geo_accession = GSM3198946 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446621 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198947 !Sample_title = cy80_CD45_neg_C05_S413_comb !Sample_geo_accession = GSM3198947 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446620 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198948 !Sample_title = cy58_1_CD45_pos_E02_S626_comb !Sample_geo_accession = GSM3198948 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446619 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198949 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_D02_S518_comb !Sample_geo_accession = GSM3198949 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446618 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198950 !Sample_title = Cy81_FNA_CD45_F10_S262_comb !Sample_geo_accession = GSM3198950 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446649 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198951 !Sample_title = Cy74_CD45_E02_S434_comb !Sample_geo_accession = GSM3198951 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446648 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198952 !Sample_title = cy80_CD45_pos_PD1_pos_B09_S117_comb !Sample_geo_accession = GSM3198952 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446711 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198953 !Sample_title = CY75_1FNA_A03_S483_comb !Sample_geo_accession = GSM3198953 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446710 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198954 !Sample_title = cy80_Cd45_pos_PD1_pos_C11_S71_comb !Sample_geo_accession = GSM3198954 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446709 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198955 !Sample_title = cy79_p4_CD45_pos_PD1_pos_H03_S375_comb !Sample_geo_accession = GSM3198955 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446708 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198956 !Sample_title = Cy81_FNA_CD45_B01_S205_comb !Sample_geo_accession = GSM3198956 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446707 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198957 !Sample_title = cy79_p5_CD45_pos_PD1_pos_B01_S589_comb !Sample_geo_accession = GSM3198957 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446706 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198958 !Sample_title = CY58_1_CD45_D06_S1002_comb !Sample_geo_accession = GSM3198958 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446705 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198959 !Sample_title = Cy72_CD45_D04_S712_comb !Sample_geo_accession = GSM3198959 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446704 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198960 !Sample_title = cy80_Cd45_pos_PD1_pos_D09_S93_comb !Sample_geo_accession = GSM3198960 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446703 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198961 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_C01_S217_comb !Sample_geo_accession = GSM3198961 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446702 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198962 !Sample_title = Cy72_CD45_C12_S708_comb !Sample_geo_accession = GSM3198962 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446701 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198963 !Sample_title = cy80_Cd45_pos_PD1_pos_B08_S44_comb !Sample_geo_accession = GSM3198963 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446700 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198964 !Sample_title = Cy72_CD45_E04_S724_comb !Sample_geo_accession = GSM3198964 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446699 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198965 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_B05_S401_comb !Sample_geo_accession = GSM3198965 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446698 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198966 !Sample_title = cy58_1_CD45_pos_F01_S637_comb !Sample_geo_accession = GSM3198966 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446697 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198967 !Sample_title = CY89A_CD45_POS_10_H04_S280_comb !Sample_geo_accession = GSM3198967 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446696 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198968 !Sample_title = cy53_1_CD45_pos_1_E07_S55_comb !Sample_geo_accession = GSM3198968 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446695 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198969 !Sample_title = cy72_CD45_pos_B10_S886_comb !Sample_geo_accession = GSM3198969 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446694 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198970 !Sample_title = cy53_1_CD45_pos_1_C06_S30_comb !Sample_geo_accession = GSM3198970 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446693 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198971 !Sample_title = cy79_p3_CD45_pos_PD1_neg_C09_S129_comb !Sample_geo_accession = GSM3198971 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446692 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198972 !Sample_title = CY89COREQ1_D06_S330_comb !Sample_geo_accession = GSM3198972 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446691 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198973 !Sample_title = Cy81_FNA_CD45_B02_S206_comb !Sample_geo_accession = GSM3198973 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446690 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198974 !Sample_title = cy80_CD45_pos_PD1_pos_H09_S189_comb !Sample_geo_accession = GSM3198974 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446689 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198975 !Sample_title = cy88_cd_45_pos_3_C09_S609_comb !Sample_geo_accession = GSM3198975 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446688 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198976 !Sample_title = CY89A_Core_15_G10_S82_comb !Sample_geo_accession = GSM3198976 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446687 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198977 !Sample_title = cy80_Cd45_pos_PD1_pos_S184_H04_S184_comb !Sample_geo_accession = GSM3198977 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446686 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198978 !Sample_title = cy80_Cd45_pos_Pd1_neg_S290_E02_S290_comb !Sample_geo_accession = GSM3198978 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446685 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198979 !Sample_title = Cy74_CD45_D10_S430_comb !Sample_geo_accession = GSM3198979 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446684 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198980 !Sample_title = cy72_CD45_pos_F06_S930_comb !Sample_geo_accession = GSM3198980 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446653 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198981 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_E10_S1018_comb !Sample_geo_accession = GSM3198981 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446652 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198982 !Sample_title = Cy72_CD45_D11_S719_comb !Sample_geo_accession = GSM3198982 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446651 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198983 !Sample_title = cy84_Primary_CD45_pos_G05_S461_comb !Sample_geo_accession = GSM3198983 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446650 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198984 !Sample_title = cy80_Cd45_pos_PD1_pos_B12_S48_comb !Sample_geo_accession = GSM3198984 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446617 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198985 !Sample_title = cy88_cd_45_pos_3_B09_S597_comb !Sample_geo_accession = GSM3198985 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446682 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198986 !Sample_title = Cy72_CD45_H03_S759_comb !Sample_geo_accession = GSM3198986 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446681 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198987 !Sample_title = cy58_1_CD45_pos_D02_S614_comb !Sample_geo_accession = GSM3198987 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446680 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198988 !Sample_title = cy72_CD45_pos_E09_S921_comb !Sample_geo_accession = GSM3198988 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446679 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198989 !Sample_title = Cy81_FNA_CD45_H02_S278_comb !Sample_geo_accession = GSM3198989 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446678 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198990 !Sample_title = cy79_p5_CD45_pos_PD1_pos_D06_S618_comb !Sample_geo_accession = GSM3198990 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446677 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198991 !Sample_title = CY89A_CD45_POS_6_C02_S122_comb !Sample_geo_accession = GSM3198991 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446676 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198992 !Sample_title = cy60_1_cd_45_pos_4_H03_S87_comb !Sample_geo_accession = GSM3198992 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446675 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198993 !Sample_title = cy80_CD45_pos_PD1_pos_B03_S111_comb !Sample_geo_accession = GSM3198993 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446674 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198994 !Sample_title = cy94_cd45pos_4_A09_S9_comb !Sample_geo_accession = GSM3198994 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446673 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198995 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_A10_S394_comb !Sample_geo_accession = GSM3198995 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446672 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198996 !Sample_title = CY88CD45POS_7_B03_S207_comb !Sample_geo_accession = GSM3198996 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446671 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198997 !Sample_title = cy80_CD45_pos_PD1_pos_E10_S154_comb !Sample_geo_accession = GSM3198997 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446670 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198998 !Sample_title = cy79_p5_CD45_pos_PD1_pos_C05_S605_comb !Sample_geo_accession = GSM3198998 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446669 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3198999 !Sample_title = CY89A_CD45_POS_6_B10_S118_comb !Sample_geo_accession = GSM3198999 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446668 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199000 !Sample_title = CY89COREQ1_C04_S316_comb !Sample_geo_accession = GSM3199000 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446667 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199001 !Sample_title = CY89A_Core_15_F01_S61_comb !Sample_geo_accession = GSM3199001 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446666 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199002 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_D11_S239_comb !Sample_geo_accession = GSM3199002 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446665 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199003 !Sample_title = cy84_Primary_CD45_pos_E09_S441_comb !Sample_geo_accession = GSM3199003 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446664 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199004 !Sample_title = cy88_cd_45_pos_E07_S439_comb !Sample_geo_accession = GSM3199004 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446663 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199005 !Sample_title = cy80_CD45_pos_PD1_pos_B05_S113_comb !Sample_geo_accession = GSM3199005 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446662 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199006 !Sample_title = cy84_Primary_CD45_pos_H10_S478_comb !Sample_geo_accession = GSM3199006 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446661 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199007 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_G12_S468_comb !Sample_geo_accession = GSM3199007 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446660 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199008 !Sample_title = cy74_CD45_pos_E04_S724_comb !Sample_geo_accession = GSM3199008 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446659 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199009 !Sample_title = cy79_p5_CD45_pos_PD1_pos_D01_S613_comb !Sample_geo_accession = GSM3199009 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446658 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199010 !Sample_title = CY94CD45POS_1_F01_S157_comb !Sample_geo_accession = GSM3199010 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446657 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199011 !Sample_title = cy60_1_cd_45_pos_4_C01_S25_comb !Sample_geo_accession = GSM3199011 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446656 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199012 !Sample_title = cy80_Cd45_pos_PD1_pos_S160_G04_S160_comb !Sample_geo_accession = GSM3199012 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446655 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199013 !Sample_title = cy79_p4_CD45_pos_PD1_pos_F12_S360_comb !Sample_geo_accession = GSM3199013 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446654 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199014 !Sample_title = CY89A_CD45_POS_6_E10_S154_comb !Sample_geo_accession = GSM3199014 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446486 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199015 !Sample_title = cy80_CD45_pos_PD1_pos_C11_S131_comb !Sample_geo_accession = GSM3199015 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446485 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199016 !Sample_title = cy60_1_cd_45_pos_3_B07_S307_comb !Sample_geo_accession = GSM3199016 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446557 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199017 !Sample_title = CY58_1_CD45_H11_S1055_comb !Sample_geo_accession = GSM3199017 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446556 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199018 !Sample_title = cy88_cd_45_pos_3_D03_S615_comb !Sample_geo_accession = GSM3199018 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446555 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199019 !Sample_title = cy80_CD45_pos_PD1_pos_C06_S126_comb !Sample_geo_accession = GSM3199019 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446554 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199020 !Sample_title = Cy71_CD45_D05_S521_comb !Sample_geo_accession = GSM3199020 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446553 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199021 !Sample_title = CY89FNA_B10_S214_comb !Sample_geo_accession = GSM3199021 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446552 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199022 !Sample_title = cy88_cd_45_pos_F09_S453_comb !Sample_geo_accession = GSM3199022 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446551 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199023 !Sample_title = cy82_CD45_pos_2_F09_S549_comb !Sample_geo_accession = GSM3199023 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446550 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199024 !Sample_title = cy58_1_CD45_pos_H06_S666_comb !Sample_geo_accession = GSM3199024 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446549 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199025 !Sample_title = CY58_1_CD45_A03_S963_comb !Sample_geo_accession = GSM3199025 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446548 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199026 !Sample_title = cy58_1_CD45_pos_F08_S644_comb !Sample_geo_accession = GSM3199026 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446547 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199027 !Sample_title = cy82_CD45_pos_3_H10_S94_comb !Sample_geo_accession = GSM3199027 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446546 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199028 !Sample_title = cy80_CD45_neg_D04_S424_comb !Sample_geo_accession = GSM3199028 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446545 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199029 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_B06_S498_comb !Sample_geo_accession = GSM3199029 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446544 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199030 !Sample_title = CY89A_CD45_POS_6_B06_S114_comb !Sample_geo_accession = GSM3199030 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446543 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199031 !Sample_title = Cy74_CD45_E07_S439_comb !Sample_geo_accession = GSM3199031 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446542 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199032 !Sample_title = cy60_1_cd_45_pos_3_B05_S305_comb !Sample_geo_accession = GSM3199032 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446541 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199033 !Sample_title = Cy72_CD45_C02_S698_comb !Sample_geo_accession = GSM3199033 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447596 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199034 !Sample_title = CY75_1FNA_G11_S563_comb !Sample_geo_accession = GSM3199034 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447595 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199035 !Sample_title = cy53_1_CD45_pos_1_D02_S38_comb !Sample_geo_accession = GSM3199035 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446540 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199036 !Sample_title = CY89A_Core_15_C02_S26_comb !Sample_geo_accession = GSM3199036 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09446539 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199037 !Sample_title = CY89A_CD45_POS_10_G02_S266_comb !Sample_geo_accession = GSM3199037 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447599 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199038 !Sample_title = CY89A_CD45_POS_6_H08_S188_comb !Sample_geo_accession = GSM3199038 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447598 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199039 !Sample_title = cy72_CD45_pos_H05_S953_comb !Sample_geo_accession = GSM3199039 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447597 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199040 !Sample_title = cy53_1_CD45_pos_2_D06_S1002_comb !Sample_geo_accession = GSM3199040 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447594 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199041 !Sample_title = cy79_p4_CD45_pos_PD1_pos_H07_S379_comb !Sample_geo_accession = GSM3199041 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447593 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199042 !Sample_title = CY88CD45POS_2_C07_S415_comb !Sample_geo_accession = GSM3199042 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447592 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199043 !Sample_title = cy60_1_cd_45_pos_4_B12_S24_comb !Sample_geo_accession = GSM3199043 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447591 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199044 !Sample_title = CY94CD45POS_1_H11_S191_comb !Sample_geo_accession = GSM3199044 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447742 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199045 !Sample_title = cy74_CD45_pos_G08_S752_comb !Sample_geo_accession = GSM3199045 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447741 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199046 !Sample_title = CY75_1FNA_H09_S573_comb !Sample_geo_accession = GSM3199046 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447740 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199047 !Sample_title = cy79_p4_CD45_pos_PD1_pos_C09_S321_comb !Sample_geo_accession = GSM3199047 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447739 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199048 !Sample_title = cy94_cd45pos_4_A04_S4_comb !Sample_geo_accession = GSM3199048 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447738 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199049 !Sample_title = cy80_Cd45_pos_PD1_pos_S183_H03_S183_comb !Sample_geo_accession = GSM3199049 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447737 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199050 !Sample_title = CY84_PRIM_POS_ALL_6_C09_S417_comb !Sample_geo_accession = GSM3199050 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447736 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199051 !Sample_title = cy53_1_CD45_pos_1_E08_S56_comb !Sample_geo_accession = GSM3199051 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447735 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199052 !Sample_title = cy88_cd_45_pos_C11_S419_comb !Sample_geo_accession = GSM3199052 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447734 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199053 !Sample_title = Cy81_FNA_CD45_F03_S255_comb !Sample_geo_accession = GSM3199053 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447733 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199054 !Sample_title = cy72_CD45_pos_E06_S918_comb !Sample_geo_accession = GSM3199054 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447732 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199055 !Sample_title = cy80_CD45_pos_PD1_pos_H12_S192_comb !Sample_geo_accession = GSM3199055 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447731 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199056 !Sample_title = CY89COREQ1_A12_S300_comb !Sample_geo_accession = GSM3199056 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447730 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199057 !Sample_title = cy94_cd45pos_4_A01_S1_comb !Sample_geo_accession = GSM3199057 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447729 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199058 !Sample_title = cy82_CD45_pos_1_G10_S562_comb !Sample_geo_accession = GSM3199058 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447728 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199059 !Sample_title = CY89A_Core_15_H07_S91_comb !Sample_geo_accession = GSM3199059 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447727 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199060 !Sample_title = cy74_CD45_pos_F07_S739_comb !Sample_geo_accession = GSM3199060 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447726 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199061 !Sample_title = CY58_1_CD45_C02_S986_comb !Sample_geo_accession = GSM3199061 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447725 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199062 !Sample_title = Cy74_CD45_F04_S448_comb !Sample_geo_accession = GSM3199062 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447724 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199063 !Sample_title = CY89A_CD45_POS_6_C06_S126_comb !Sample_geo_accession = GSM3199063 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447723 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199064 !Sample_title = CY89FNA_F05_S257_comb !Sample_geo_accession = GSM3199064 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447722 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199065 !Sample_title = CY89COREQ1_B12_S312_comb !Sample_geo_accession = GSM3199065 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447721 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199066 !Sample_title = CY89A_CD45_POS_10_B12_S216_comb !Sample_geo_accession = GSM3199066 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447720 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199067 !Sample_title = Cy74_CD45_G10_S466_comb !Sample_geo_accession = GSM3199067 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447719 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199068 !Sample_title = cy84_Primary_CD45_pos_H11_S479_comb !Sample_geo_accession = GSM3199068 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447718 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199069 !Sample_title = cy74_CD45_pos_E11_S731_comb !Sample_geo_accession = GSM3199069 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447717 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199070 !Sample_title = cy80_CD45_neg_E10_S442_comb !Sample_geo_accession = GSM3199070 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447716 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199071 !Sample_title = CY94CD45POS_1_D12_S144_comb !Sample_geo_accession = GSM3199071 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447715 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199072 !Sample_title = cy84_Met_CD45_pos_G01_S937_comb !Sample_geo_accession = GSM3199072 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447788 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199073 !Sample_title = cy58_1_CD45_pos_A05_S581_comb !Sample_geo_accession = GSM3199073 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447787 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199074 !Sample_title = cy58_1_CD45_pos_E07_S631_comb !Sample_geo_accession = GSM3199074 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447786 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199075 !Sample_title = cy94_cd45neg_cd90pos_C04_S316_comb !Sample_geo_accession = GSM3199075 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447785 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199076 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_F07_S547_comb !Sample_geo_accession = GSM3199076 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447784 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199077 !Sample_title = Cy81_FNA_CD45_C03_S219_comb !Sample_geo_accession = GSM3199077 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447783 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199078 !Sample_title = CY89A_Core_15_A11_S11_comb !Sample_geo_accession = GSM3199078 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447782 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199079 !Sample_title = cy60_1_cd_45_pos_4_D11_S47_comb !Sample_geo_accession = GSM3199079 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447781 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199080 !Sample_title = Cy72_CD45_F03_S735_comb !Sample_geo_accession = GSM3199080 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447780 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199081 !Sample_title = CY75_1FNA_A10_S490_comb !Sample_geo_accession = GSM3199081 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447779 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199082 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_D02_S230_comb !Sample_geo_accession = GSM3199082 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447778 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199083 !Sample_title = cy94_cd45pos_D02_S134_comb !Sample_geo_accession = GSM3199083 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447777 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199084 !Sample_title = cy58_1_CD45_pos_B09_S597_comb !Sample_geo_accession = GSM3199084 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447776 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199085 !Sample_title = cy79_p5_CD45_pos_PD1_pos_E06_S630_comb !Sample_geo_accession = GSM3199085 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447775 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199086 !Sample_title = cy79_p5_CD45_pos_PD1_pos_B10_S598_comb !Sample_geo_accession = GSM3199086 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447774 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199087 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_D07_S1003_comb !Sample_geo_accession = GSM3199087 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447773 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199088 !Sample_title = CY88CD45POS_2_D07_S427_comb !Sample_geo_accession = GSM3199088 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447772 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199089 !Sample_title = CY58_1_CD45_B11_S983_comb !Sample_geo_accession = GSM3199089 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447771 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199090 !Sample_title = Cy81_FNA_CD45_E05_S245_comb !Sample_geo_accession = GSM3199090 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447770 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199091 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_G01_S457_comb !Sample_geo_accession = GSM3199091 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447769 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199092 !Sample_title = cy53_1_CD45_pos_1_C10_S34_comb !Sample_geo_accession = GSM3199092 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447768 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199093 !Sample_title = cy94_cd45pos_4_A06_S6_comb !Sample_geo_accession = GSM3199093 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447767 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199094 !Sample_title = CY88CD45_150813_C05_S317_comb !Sample_geo_accession = GSM3199094 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447766 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199095 !Sample_title = Cy72_CD45_G06_S750_comb !Sample_geo_accession = GSM3199095 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447765 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199096 !Sample_title = cy53_1_CD45_pos_2_H11_S1055_comb !Sample_geo_accession = GSM3199096 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447764 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199097 !Sample_title = cy80_CD45_pos_PD1_pos_G08_S176_comb !Sample_geo_accession = GSM3199097 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447763 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199098 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_G06_S270_comb !Sample_geo_accession = GSM3199098 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447762 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199099 !Sample_title = CY89COREQ1_B11_S311_comb !Sample_geo_accession = GSM3199099 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447761 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199100 !Sample_title = cy60_1_cd_45_pos_3_H01_S373_comb !Sample_geo_accession = GSM3199100 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447760 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199101 !Sample_title = cy72_CD45_pos_G02_S938_comb !Sample_geo_accession = GSM3199101 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447759 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199102 !Sample_title = cy60_1_cd_45_pos_4_E04_S52_comb !Sample_geo_accession = GSM3199102 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447758 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199103 !Sample_title = cy94_cd45pos_4_G01_S73_comb !Sample_geo_accession = GSM3199103 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447757 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199104 !Sample_title = CY88CD45POS_7_E07_S247_comb !Sample_geo_accession = GSM3199104 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447756 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199105 !Sample_title = cy88_cd_45_pos_E11_S443_comb !Sample_geo_accession = GSM3199105 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447755 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199106 !Sample_title = cy94_cd45pos_4_G09_S81_comb !Sample_geo_accession = GSM3199106 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447754 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199107 !Sample_title = CY89A_CD45_POS_6_F08_S164_comb !Sample_geo_accession = GSM3199107 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447753 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199108 !Sample_title = cy79_p4_CD45_pos_PD1_pos_E11_S347_comb !Sample_geo_accession = GSM3199108 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447752 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199109 !Sample_title = cy94_cd45pos_4_E08_S56_comb !Sample_geo_accession = GSM3199109 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447751 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199110 !Sample_title = cy79_p5_CD45_pos_PD1_pos_A03_S579_comb !Sample_geo_accession = GSM3199110 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447750 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199111 !Sample_title = Cy71_CD45_F04_S544_comb !Sample_geo_accession = GSM3199111 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447823 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199112 !Sample_title = cy80_CD45_pos_PD1_pos_E11_S155_comb !Sample_geo_accession = GSM3199112 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447822 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199113 !Sample_title = cy80_CD45_neg_C04_S412_comb !Sample_geo_accession = GSM3199113 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447821 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199114 !Sample_title = CY88CD45POS_7_G02_S266_comb !Sample_geo_accession = GSM3199114 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447820 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199115 !Sample_title = CY58_1_CD45_D07_S1003_comb !Sample_geo_accession = GSM3199115 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447819 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199116 !Sample_title = cy80_Cd45_pos_PD1_pos_S187_H07_S187_comb !Sample_geo_accession = GSM3199116 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447818 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199117 !Sample_title = Cy71_CD45_E07_S535_comb !Sample_geo_accession = GSM3199117 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447817 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199118 !Sample_title = cy60_1_cd_45_pos_3_F01_S349_comb !Sample_geo_accession = GSM3199118 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447816 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199119 !Sample_title = CY58_1_CD45_F06_S1026_comb !Sample_geo_accession = GSM3199119 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447815 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199120 !Sample_title = cy79_p4_CD45_pos_PD1_pos_F10_S358_comb !Sample_geo_accession = GSM3199120 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447814 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199121 !Sample_title = cy82_CD45_pos_3_C07_S31_comb !Sample_geo_accession = GSM3199121 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447813 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199122 !Sample_title = cy88_cd_45_pos_B09_S405_comb !Sample_geo_accession = GSM3199122 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447812 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199123 !Sample_title = cy74_CD45_pos_G09_S753_comb !Sample_geo_accession = GSM3199123 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447811 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199124 !Sample_title = cy79_p4_CD45_pos_PD1_pos_F05_S353_comb !Sample_geo_accession = GSM3199124 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447810 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199125 !Sample_title = Cy81_FNA_CD45_B06_S210_comb !Sample_geo_accession = GSM3199125 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447809 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199126 !Sample_title = cy74_CD45_pos_E05_S725_comb !Sample_geo_accession = GSM3199126 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447808 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199127 !Sample_title = cy80_CD45_pos_PD1_pos_D10_S142_comb !Sample_geo_accession = GSM3199127 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447807 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199128 !Sample_title = CY88CD45POS_7_B09_S213_comb !Sample_geo_accession = GSM3199128 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447806 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199129 !Sample_title = cy88_cd_45_pos_3_D09_S621_comb !Sample_geo_accession = GSM3199129 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447805 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199130 !Sample_title = cy94_cd45pos_B07_S115_comb !Sample_geo_accession = GSM3199130 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447804 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199131 !Sample_title = cy58_1_CD45_pos_H04_S664_comb !Sample_geo_accession = GSM3199131 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447803 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199132 !Sample_title = cy88_cd_45_pos_F03_S447_comb !Sample_geo_accession = GSM3199132 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447802 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199133 !Sample_title = Cy81_FNA_CD45_A10_S202_comb !Sample_geo_accession = GSM3199133 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447801 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199134 !Sample_title = CY89A_CD45_POS_10_B07_S211_comb !Sample_geo_accession = GSM3199134 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447800 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199135 !Sample_title = CY89FNA_A08_S200_comb !Sample_geo_accession = GSM3199135 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447799 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199136 !Sample_title = cy58_1_CD45_pos_B11_S599_comb !Sample_geo_accession = GSM3199136 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447798 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199137 !Sample_title = cy79_p4_CD45_pos_PD1_pos_F11_S359_comb !Sample_geo_accession = GSM3199137 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447797 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199138 !Sample_title = cy53_1_CD45_pos_1_E12_S60_comb !Sample_geo_accession = GSM3199138 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447796 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199139 !Sample_title = Cy72_CD45_A10_S682_comb !Sample_geo_accession = GSM3199139 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447795 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199140 !Sample_title = cy79_p5_CD45_pos_PD1_pos_C02_S602_comb !Sample_geo_accession = GSM3199140 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447794 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199141 !Sample_title = cy74_CD45_pos_E08_S728_comb !Sample_geo_accession = GSM3199141 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447793 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199142 !Sample_title = Cy72_CD45_A12_S684_comb !Sample_geo_accession = GSM3199142 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447792 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199143 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_G04_S268_comb !Sample_geo_accession = GSM3199143 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447791 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199144 !Sample_title = cy60_1_cd_45_pos_4_H02_S86_comb !Sample_geo_accession = GSM3199144 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447790 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199145 !Sample_title = CY58_1_CD45_G02_S1034_comb !Sample_geo_accession = GSM3199145 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447789 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199146 !Sample_title = cy53_1_CD45_pos_2_A03_S963_comb !Sample_geo_accession = GSM3199146 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450321 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199147 !Sample_title = CY58_1_CD45_G03_S1035_comb !Sample_geo_accession = GSM3199147 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450320 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199148 !Sample_title = Cy72_CD45_D10_S718_comb !Sample_geo_accession = GSM3199148 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450319 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199149 !Sample_title = CY94CD45POS_1_A03_S99_comb !Sample_geo_accession = GSM3199149 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450318 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199150 !Sample_title = cy79_p4_CD45_pos_PD1_pos_A08_S296_comb !Sample_geo_accession = GSM3199150 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450316 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199151 !Sample_title = cy58_1_CD45_pos_B04_S592_comb !Sample_geo_accession = GSM3199151 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450315 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199152 !Sample_title = cy79_p4_CD45_pos_PD1_pos_F09_S357_comb !Sample_geo_accession = GSM3199152 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450375 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199153 !Sample_title = CY89A_Core_15_B12_S24_comb !Sample_geo_accession = GSM3199153 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450374 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199154 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_D01_S229_comb !Sample_geo_accession = GSM3199154 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450373 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199155 !Sample_title = cy53_1_CD45_pos_2_H01_S1045_comb !Sample_geo_accession = GSM3199155 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450317 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199156 !Sample_title = cy58_1_CD45_pos_H07_S667_comb !Sample_geo_accession = GSM3199156 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450372 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199157 !Sample_title = cy79_p5_CD45_pos_PD1_pos_B03_S591_comb !Sample_geo_accession = GSM3199157 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450371 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199158 !Sample_title = CY89A_Core_15_D12_S48_comb !Sample_geo_accession = GSM3199158 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450370 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199159 !Sample_title = cy53_1_CD45_pos_1_F07_S67_comb !Sample_geo_accession = GSM3199159 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450369 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199160 !Sample_title = cy72_CD45_pos_H06_S954_comb !Sample_geo_accession = GSM3199160 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450368 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199161 !Sample_title = cy79_p5_CD45_pos_PD1_pos_G07_S655_comb !Sample_geo_accession = GSM3199161 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450367 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199162 !Sample_title = cy79_p5_CD45_pos_PD1_pos_D05_S617_comb !Sample_geo_accession = GSM3199162 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450366 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199163 !Sample_title = cy79_p5_CD45_pos_PD1_pos_A08_S584_comb !Sample_geo_accession = GSM3199163 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450365 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199164 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_A11_S395_comb !Sample_geo_accession = GSM3199164 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450364 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199165 !Sample_title = cy88_cd45pos_5_E01_S241_comb !Sample_geo_accession = GSM3199165 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450363 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199166 !Sample_title = cy80_Cd45_pos_Pd1_neg_S295_E07_S295_comb !Sample_geo_accession = GSM3199166 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450362 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199167 !Sample_title = cy94_cd45neg_cd90pos_D06_S330_comb !Sample_geo_accession = GSM3199167 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450361 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199168 !Sample_title = cy80_CD45_pos_PD1_pos_G11_S179_comb !Sample_geo_accession = GSM3199168 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450360 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199169 !Sample_title = cy74_CD45_pos_D10_S718_comb !Sample_geo_accession = GSM3199169 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450359 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199170 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_H02_S278_comb !Sample_geo_accession = GSM3199170 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450358 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199171 !Sample_title = Cy72_CD45_E01_S721_comb !Sample_geo_accession = GSM3199171 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450357 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199172 !Sample_title = CY88CD45POS_7_H03_S279_comb !Sample_geo_accession = GSM3199172 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450356 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199173 !Sample_title = cy79_p4_CD45_pos_PD1_pos_G11_S371_comb !Sample_geo_accession = GSM3199173 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450355 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199174 !Sample_title = cy58_1_CD45_pos_G06_S654_comb !Sample_geo_accession = GSM3199174 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450354 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199175 !Sample_title = cy94_cd45pos_4_E07_S55_comb !Sample_geo_accession = GSM3199175 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450353 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199176 !Sample_title = CY89A_Core_15_G08_S80_comb !Sample_geo_accession = GSM3199176 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450347 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199177 !Sample_title = cy94_cd45pos_4_B08_S20_comb !Sample_geo_accession = GSM3199177 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450346 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199178 !Sample_title = Cy81_FNA_CD45_F07_S259_comb !Sample_geo_accession = GSM3199178 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450345 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199179 !Sample_title = Cy72_CD45_B12_S696_comb !Sample_geo_accession = GSM3199179 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450344 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199180 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_G03_S267_comb !Sample_geo_accession = GSM3199180 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450343 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199181 !Sample_title = cy79_p4_CD45_pos_PD1_pos_A02_S290_comb !Sample_geo_accession = GSM3199181 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450342 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199182 !Sample_title = cy94_cd45pos_4_E11_S59_comb !Sample_geo_accession = GSM3199182 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450341 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199183 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_G02_S266_comb !Sample_geo_accession = GSM3199183 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450340 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199184 !Sample_title = cy80_Cd45_pos_Pd1_neg_S299_E11_S299_comb !Sample_geo_accession = GSM3199184 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450338 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199185 !Sample_title = cy60_1_cd_45_pos_3_E09_S345_comb !Sample_geo_accession = GSM3199185 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450337 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199186 !Sample_title = cy53_1_CD45_pos_1_D09_S45_comb !Sample_geo_accession = GSM3199186 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450336 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199187 !Sample_title = cy80_CD45_pos_PD1_pos_D01_S133_comb !Sample_geo_accession = GSM3199187 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450335 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199188 !Sample_title = cy79_p4_CD45_pos_PD1_pos_E08_S344_comb !Sample_geo_accession = GSM3199188 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450334 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199189 !Sample_title = cy88_cd_45_pos_F04_S448_comb !Sample_geo_accession = GSM3199189 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450333 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199190 !Sample_title = cy79_p4_CD45_pos_PD1_pos_G04_S364_comb !Sample_geo_accession = GSM3199190 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450332 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199191 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_F05_S449_comb !Sample_geo_accession = GSM3199191 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450331 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199192 !Sample_title = CY88CD45_150813_D01_S325_comb !Sample_geo_accession = GSM3199192 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450330 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199193 !Sample_title = cy80_CD45_neg_G08_S464_comb !Sample_geo_accession = GSM3199193 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450329 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199194 !Sample_title = cy80_Cd45_pos_PD1_pos_D05_S89_comb !Sample_geo_accession = GSM3199194 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450328 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199195 !Sample_title = CY58_1_CD45_A08_S968_comb !Sample_geo_accession = GSM3199195 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450327 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199196 !Sample_title = CY94CD45POS_1_D01_S133_comb !Sample_geo_accession = GSM3199196 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450326 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199197 !Sample_title = Cy72_CD45_H08_S764_comb !Sample_geo_accession = GSM3199197 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450325 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199198 !Sample_title = CY89A_Core_15_H04_S88_comb !Sample_geo_accession = GSM3199198 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450324 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199199 !Sample_title = cy79_p5_CD45_pos_PD1_pos_H01_S661_comb !Sample_geo_accession = GSM3199199 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450323 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199200 !Sample_title = cy79_p4_CD45_pos_PD1_pos_D09_S333_comb !Sample_geo_accession = GSM3199200 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450322 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199201 !Sample_title = cy79_p3_CD45_pos_PD1_neg_F12_S168_comb !Sample_geo_accession = GSM3199201 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450186 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199202 !Sample_title = cy79_p5_CD45_pos_PD1_pos_H04_S664_comb !Sample_geo_accession = GSM3199202 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450185 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199203 !Sample_title = cy53_1_CD45_pos_1_C12_S36_comb !Sample_geo_accession = GSM3199203 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450184 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199204 !Sample_title = cy74_CD45_pos_D06_S714_comb !Sample_geo_accession = GSM3199204 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450183 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199205 !Sample_title = CY84_PRIM_POS_ALL_7_G05_S269_comb !Sample_geo_accession = GSM3199205 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450182 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199206 !Sample_title = cy80_CD45_pos_PD1_pos_A12_S108_comb !Sample_geo_accession = GSM3199206 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450240 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199207 !Sample_title = cy80_Cd45_pos_PD1_pos_S118_E10_S118_comb !Sample_geo_accession = GSM3199207 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450239 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199208 !Sample_title = cy60_1_cd_45_pos_3_G03_S363_comb !Sample_geo_accession = GSM3199208 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450238 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199209 !Sample_title = CY58_1_CD45_C07_S991_comb !Sample_geo_accession = GSM3199209 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450237 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199210 !Sample_title = cy74_CD45_pos_H05_S761_comb !Sample_geo_accession = GSM3199210 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450236 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199211 !Sample_title = CY58_1_CD45_G01_S1033_comb !Sample_geo_accession = GSM3199211 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450232 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199212 !Sample_title = cy80_Cd45_pos_PD1_pos_S167_G11_S167_comb !Sample_geo_accession = GSM3199212 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450231 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199213 !Sample_title = Cy81_FNA_CD45_G06_S270_comb !Sample_geo_accession = GSM3199213 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450230 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199214 !Sample_title = Cy81_FNA_CD45_A11_S203_comb !Sample_geo_accession = GSM3199214 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450229 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199215 !Sample_title = Cy71_CD45_H09_S573_comb !Sample_geo_accession = GSM3199215 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450235 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199216 !Sample_title = Cy72_CD45_G10_S754_comb !Sample_geo_accession = GSM3199216 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450234 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199217 !Sample_title = CY88CD45_150813_C09_S321_comb !Sample_geo_accession = GSM3199217 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450233 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199218 !Sample_title = cy79_p4_CD45_pos_PD1_pos_A07_S295_comb !Sample_geo_accession = GSM3199218 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450228 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199219 !Sample_title = cy58_1_CD45_pos_E01_S625_comb !Sample_geo_accession = GSM3199219 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450227 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199220 !Sample_title = Cy74_CD45_G09_S465_comb !Sample_geo_accession = GSM3199220 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450226 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199221 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_F04_S256_comb !Sample_geo_accession = GSM3199221 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450225 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199222 !Sample_title = cy79_p4_CD45_pos_PD1_pos_H12_S384_comb !Sample_geo_accession = GSM3199222 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450224 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199223 !Sample_title = cy94_cd45pos_4_H07_S91_comb !Sample_geo_accession = GSM3199223 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450223 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199224 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_D12_S528_comb !Sample_geo_accession = GSM3199224 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450222 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199225 !Sample_title = cy60_1_cd_45_pos_4_A01_S1_comb !Sample_geo_accession = GSM3199225 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450221 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199226 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_D03_S231_comb !Sample_geo_accession = GSM3199226 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450220 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199227 !Sample_title = cy79_p4_CD45_pos_PD1_pos_H08_S380_comb !Sample_geo_accession = GSM3199227 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450219 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199228 !Sample_title = CY94CD45POS_1_A01_S97_comb !Sample_geo_accession = GSM3199228 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450218 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199229 !Sample_title = CY89A_CD45_POS_6_B01_S109_comb !Sample_geo_accession = GSM3199229 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450217 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199230 !Sample_title = cy74_CD45_pos_B08_S692_comb !Sample_geo_accession = GSM3199230 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450216 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199231 !Sample_title = Cy74_CD45_F11_S455_comb !Sample_geo_accession = GSM3199231 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450255 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199232 !Sample_title = cy72_CD45_pos_H02_S950_comb !Sample_geo_accession = GSM3199232 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450254 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199233 !Sample_title = cy79_p4_CD45_pos_PD1_pos_E03_S339_comb !Sample_geo_accession = GSM3199233 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450253 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199234 !Sample_title = cy79_p5_CD45_pos_PD1_pos_A11_S587_comb !Sample_geo_accession = GSM3199234 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450252 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199235 !Sample_title = cy60_1_cd_45_pos_4_H07_S91_comb !Sample_geo_accession = GSM3199235 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450251 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199236 !Sample_title = cy84_Primary_CD45_pos_C03_S411_comb !Sample_geo_accession = GSM3199236 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450250 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199237 !Sample_title = cy79_p5_CD45_pos_PD1_pos_A02_S578_comb !Sample_geo_accession = GSM3199237 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450249 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199238 !Sample_title = CY88CD45POS_7_D02_S230_comb !Sample_geo_accession = GSM3199238 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450248 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199239 !Sample_title = cy72_CD45_pos_F12_S936_comb !Sample_geo_accession = GSM3199239 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450247 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199240 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_B04_S208_comb !Sample_geo_accession = GSM3199240 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450246 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199241 !Sample_title = CY58_1_CD45_H10_S1054_comb !Sample_geo_accession = GSM3199241 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450245 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199242 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_C02_S506_comb !Sample_geo_accession = GSM3199242 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450244 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199243 !Sample_title = cy80_CD45_neg_B12_S408_comb !Sample_geo_accession = GSM3199243 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450243 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199244 !Sample_title = cy79_p4_CD45_pos_PD1_pos_H02_S374_comb !Sample_geo_accession = GSM3199244 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450242 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199245 !Sample_title = cy53_1_CD45_pos_1_G04_S76_comb !Sample_geo_accession = GSM3199245 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450241 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199246 !Sample_title = cy58_1_CD45_pos_C02_S602_comb !Sample_geo_accession = GSM3199246 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450314 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199247 !Sample_title = cy80_Cd45_pos_PD1_pos_D06_S90_comb !Sample_geo_accession = GSM3199247 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450313 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199248 !Sample_title = cy72_CD45_pos_E11_S923_comb !Sample_geo_accession = GSM3199248 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450312 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199249 !Sample_title = cy74_CD45_pos_C01_S697_comb !Sample_geo_accession = GSM3199249 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450311 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199250 !Sample_title = cy82_CD45_pos_1_C04_S508_comb !Sample_geo_accession = GSM3199250 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450310 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199251 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_C06_S414_comb !Sample_geo_accession = GSM3199251 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450309 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199252 !Sample_title = cy94_cd45pos_A12_S108_comb !Sample_geo_accession = GSM3199252 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450308 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199253 !Sample_title = Cy72_CD45_E03_S723_comb !Sample_geo_accession = GSM3199253 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450307 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199254 !Sample_title = cy84_Primary_CD45_pos_A01_S385_comb !Sample_geo_accession = GSM3199254 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450306 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199255 !Sample_title = cy58_1_CD45_pos_G07_S655_comb !Sample_geo_accession = GSM3199255 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450305 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199256 !Sample_title = Cy72_CD45_B11_S695_comb !Sample_geo_accession = GSM3199256 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450304 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199257 !Sample_title = Cy74_CD45_D09_S429_comb !Sample_geo_accession = GSM3199257 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450303 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199258 !Sample_title = cy88_cd45pos_5_B03_S207_comb !Sample_geo_accession = GSM3199258 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450302 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199259 !Sample_title = Cy72_CD45_H09_S765_comb !Sample_geo_accession = GSM3199259 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450301 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199260 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_E08_S536_comb !Sample_geo_accession = GSM3199260 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450300 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199261 !Sample_title = cy58_1_CD45_pos_F07_S643_comb !Sample_geo_accession = GSM3199261 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450299 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199262 !Sample_title = cy74_CD45_pos_F09_S741_comb !Sample_geo_accession = GSM3199262 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450298 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199263 !Sample_title = cy80_Cd45_pos_PD1_pos_A08_S20_comb !Sample_geo_accession = GSM3199263 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450297 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199264 !Sample_title = cy82_CD45_pos_2_D04_S520_comb !Sample_geo_accession = GSM3199264 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450296 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199265 !Sample_title = cy79_p5_CD45_pos_PD1_pos_B02_S590_comb !Sample_geo_accession = GSM3199265 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450295 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199266 !Sample_title = Cy72_CD45_G07_S751_comb !Sample_geo_accession = GSM3199266 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450294 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199267 !Sample_title = cy88_cd_45_pos_3_C07_S607_comb !Sample_geo_accession = GSM3199267 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450293 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199268 !Sample_title = Cy72_CD45_G05_S749_comb !Sample_geo_accession = GSM3199268 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450292 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199269 !Sample_title = cy80_CD45_neg_C07_S415_comb !Sample_geo_accession = GSM3199269 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450291 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199270 !Sample_title = Cy71_CD45_D03_S519_comb !Sample_geo_accession = GSM3199270 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450290 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199271 !Sample_title = cy79_p3_CD45_pos_PD1_neg_E07_S151_comb !Sample_geo_accession = GSM3199271 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450289 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199272 !Sample_title = cy88_cd_45_pos_E03_S435_comb !Sample_geo_accession = GSM3199272 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450288 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199273 !Sample_title = Cy81_FNA_CD45_F05_S257_comb !Sample_geo_accession = GSM3199273 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450283 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199274 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_D10_S238_comb !Sample_geo_accession = GSM3199274 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450282 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199275 !Sample_title = cy58_1_CD45_pos_C10_S610_comb !Sample_geo_accession = GSM3199275 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450287 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199276 !Sample_title = cy79_p4_CD45_pos_PD1_pos_B06_S306_comb !Sample_geo_accession = GSM3199276 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450286 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199277 !Sample_title = cy80_CD45_pos_PD1_pos_G04_S172_comb !Sample_geo_accession = GSM3199277 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450285 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199278 !Sample_title = Cy72_CD45_C11_S707_comb !Sample_geo_accession = GSM3199278 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450284 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199279 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_B07_S403_comb !Sample_geo_accession = GSM3199279 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450281 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199280 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_H11_S287_comb !Sample_geo_accession = GSM3199280 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450352 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199281 !Sample_title = cy88_cd45pos_5_E05_S245_comb !Sample_geo_accession = GSM3199281 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450351 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199282 !Sample_title = cy58_1_CD45_pos_H10_S670_comb !Sample_geo_accession = GSM3199282 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450350 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199283 !Sample_title = cy80_CD45_pos_PD1_pos_E06_S150_comb !Sample_geo_accession = GSM3199283 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450349 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199284 !Sample_title = cy80_Cd45_pos_PD1_pos_B10_S46_comb !Sample_geo_accession = GSM3199284 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450348 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199285 !Sample_title = CY88CD45POS_2_E05_S437_comb !Sample_geo_accession = GSM3199285 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450059 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199286 !Sample_title = cy94_cd45pos_H10_S190_comb !Sample_geo_accession = GSM3199286 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450058 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199287 !Sample_title = cy53_1_CD45_pos_1_G03_S75_comb !Sample_geo_accession = GSM3199287 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450057 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199288 !Sample_title = cy79_p3_CD45_pos_PD1_neg_E02_S146_comb !Sample_geo_accession = GSM3199288 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450056 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199289 !Sample_title = CY89A_CD45_POS_10_F02_S254_comb !Sample_geo_accession = GSM3199289 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450055 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199290 !Sample_title = cy72_CD45_pos_F05_S929_comb !Sample_geo_accession = GSM3199290 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450054 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199291 !Sample_title = cy53_1_CD45_pos_2_B04_S976_comb !Sample_geo_accession = GSM3199291 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450053 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199292 !Sample_title = cy53_1_CD45_pos_1_B12_S24_comb !Sample_geo_accession = GSM3199292 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450052 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199293 !Sample_title = cy79_p5_CD45_pos_PD1_pos_D10_S622_comb !Sample_geo_accession = GSM3199293 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450051 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199294 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_B06_S402_comb !Sample_geo_accession = GSM3199294 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450050 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199295 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_G08_S464_comb !Sample_geo_accession = GSM3199295 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450049 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199296 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_F06_S450_comb !Sample_geo_accession = GSM3199296 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450048 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199297 !Sample_title = cy79_p5_CD45_pos_PD1_pos_B05_S593_comb !Sample_geo_accession = GSM3199297 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450047 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199298 !Sample_title = CY58_1_CD45_G06_S1038_comb !Sample_geo_accession = GSM3199298 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450046 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199299 !Sample_title = cy88_cd_45_pos_3_D11_S623_comb !Sample_geo_accession = GSM3199299 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450045 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199300 !Sample_title = cy80_Cd45_pos_PD1_pos_S158_G02_S158_comb !Sample_geo_accession = GSM3199300 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450044 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199301 !Sample_title = cy53_1_CD45_pos_2_D08_S1004_comb !Sample_geo_accession = GSM3199301 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450043 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199302 !Sample_title = cy79_p4_CD45_pos_PD1_pos_C02_S314_comb !Sample_geo_accession = GSM3199302 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450042 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199303 !Sample_title = CY94CD45POS_1_D10_S142_comb !Sample_geo_accession = GSM3199303 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450041 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199304 !Sample_title = cy80_CD45_pos_PD1_pos_B01_S109_comb !Sample_geo_accession = GSM3199304 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450114 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199305 !Sample_title = cy60_1_cd_45_pos_4_A09_S9_comb !Sample_geo_accession = GSM3199305 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450113 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199306 !Sample_title = cy80_Cd45_pos_PD1_pos_S114_E06_S114_comb !Sample_geo_accession = GSM3199306 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450112 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199307 !Sample_title = cy80_CD45_neg_F05_S449_comb !Sample_geo_accession = GSM3199307 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450111 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199308 !Sample_title = cy60_1_cd_45_pos_4_D09_S45_comb !Sample_geo_accession = GSM3199308 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450110 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199309 !Sample_title = cy82_CD45_pos_2_B10_S502_comb !Sample_geo_accession = GSM3199309 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450109 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199310 !Sample_title = cy88_cd_45_pos_E09_S441_comb !Sample_geo_accession = GSM3199310 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450108 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199311 !Sample_title = cy79_p4_CD45_pos_PD1_pos_H01_S373_comb !Sample_geo_accession = GSM3199311 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450107 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199312 !Sample_title = cy84_Primary_CD45_pos_C04_S412_comb !Sample_geo_accession = GSM3199312 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450106 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199313 !Sample_title = cy74_CD45_pos_D07_S715_comb !Sample_geo_accession = GSM3199313 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450105 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199314 !Sample_title = cy94_cd45pos_G06_S174_comb !Sample_geo_accession = GSM3199314 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450104 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199315 !Sample_title = cy72_CD45_pos_G06_S942_comb !Sample_geo_accession = GSM3199315 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450017 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199316 !Sample_title = cy58_1_CD45_pos_F06_S642_comb !Sample_geo_accession = GSM3199316 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450016 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199317 !Sample_title = CY58_1_CD45_G07_S1039_comb !Sample_geo_accession = GSM3199317 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450015 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199318 !Sample_title = cy80_Cd45_pos_PD1_pos_B03_S39_comb !Sample_geo_accession = GSM3199318 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450014 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199319 !Sample_title = cy94_cd45neg_cd90pos_G12_S372_comb !Sample_geo_accession = GSM3199319 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450013 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199320 !Sample_title = cy72_CD45_pos_C04_S892_comb !Sample_geo_accession = GSM3199320 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450012 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199321 !Sample_title = CY89A_Core_15_G03_S75_comb !Sample_geo_accession = GSM3199321 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450011 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199322 !Sample_title = Cy71_CD45_H03_S567_comb !Sample_geo_accession = GSM3199322 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450010 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199323 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_E12_S444_comb !Sample_geo_accession = GSM3199323 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450009 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199324 !Sample_title = cy94_cd45neg_cd90pos_F07_S355_comb !Sample_geo_accession = GSM3199324 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450008 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199325 !Sample_title = CY88CD45POS_7_E05_S245_comb !Sample_geo_accession = GSM3199325 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450007 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199326 !Sample_title = cy72_CD45_pos_E05_S917_comb !Sample_geo_accession = GSM3199326 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450006 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199327 !Sample_title = CY58_1_CD45_A02_S962_comb !Sample_geo_accession = GSM3199327 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450005 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199328 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_B10_S214_comb !Sample_geo_accession = GSM3199328 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450004 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199329 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_D08_S428_comb !Sample_geo_accession = GSM3199329 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450076 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199330 !Sample_title = CY89A_CD45_POS_10_E12_S252_comb !Sample_geo_accession = GSM3199330 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450075 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199331 !Sample_title = Cy71_CD45_F12_S552_comb !Sample_geo_accession = GSM3199331 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450074 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199332 !Sample_title = CY84_PRIM_POS_ALL_6_A08_S392_comb !Sample_geo_accession = GSM3199332 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450073 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199333 !Sample_title = Cy74_CD45_A11_S395_comb !Sample_geo_accession = GSM3199333 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450067 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199334 !Sample_title = cy80_Cd45_pos_PD1_pos_B05_S41_comb !Sample_geo_accession = GSM3199334 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450066 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199335 !Sample_title = cy94_cd45pos_4_H08_S92_comb !Sample_geo_accession = GSM3199335 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450071 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199336 !Sample_title = cy94_cd45pos_C10_S130_comb !Sample_geo_accession = GSM3199336 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450070 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199337 !Sample_title = Cy81_FNA_CD45_D06_S234_comb !Sample_geo_accession = GSM3199337 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450069 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199338 !Sample_title = cy79_p5_CD45_pos_PD1_pos_D08_S620_comb !Sample_geo_accession = GSM3199338 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450068 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199339 !Sample_title = cy84_Primary_CD45_pos_G12_S468_comb !Sample_geo_accession = GSM3199339 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450065 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199340 !Sample_title = cy72_CD45_pos_D01_S901_comb !Sample_geo_accession = GSM3199340 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450064 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199341 !Sample_title = CY88CD45_150813_B01_S301_comb !Sample_geo_accession = GSM3199341 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450063 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199342 !Sample_title = cy79_p3_CD45_pos_PD1_neg_E08_S152_comb !Sample_geo_accession = GSM3199342 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450062 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199343 !Sample_title = cy79_p4_CD45_pos_PD1_pos_D07_S331_comb !Sample_geo_accession = GSM3199343 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450061 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199344 !Sample_title = CY89A_Core_15_D10_S46_comb !Sample_geo_accession = GSM3199344 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450060 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199345 !Sample_title = cy79_p5_CD45_pos_PD1_pos_E07_S631_comb !Sample_geo_accession = GSM3199345 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450160 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199346 !Sample_title = cy80_Cd45_pos_PD1_pos_S117_E09_S117_comb !Sample_geo_accession = GSM3199346 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450159 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199347 !Sample_title = cy58_1_CD45_pos_A12_S588_comb !Sample_geo_accession = GSM3199347 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450158 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199348 !Sample_title = Cy71_CD45_D10_S526_comb !Sample_geo_accession = GSM3199348 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450157 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199349 !Sample_title = cy53_1_CD45_pos_1_C11_S35_comb !Sample_geo_accession = GSM3199349 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450156 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199350 !Sample_title = cy79_p4_CD45_pos_PD1_pos_E02_S338_comb !Sample_geo_accession = GSM3199350 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450155 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199351 !Sample_title = cy60_1_cd_45_pos_3_F06_S354_comb !Sample_geo_accession = GSM3199351 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450154 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199352 !Sample_title = cy94_cd45pos_B08_S116_comb !Sample_geo_accession = GSM3199352 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450153 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199353 !Sample_title = cy79_p5_CD45_pos_PD1_pos_D11_S623_comb !Sample_geo_accession = GSM3199353 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450152 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199354 !Sample_title = cy79_p4_CD45_pos_PD1_pos_D05_S329_comb !Sample_geo_accession = GSM3199354 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449973 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199355 !Sample_title = cy94_cd45pos_4_H03_S87_comb !Sample_geo_accession = GSM3199355 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449972 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199356 !Sample_title = cy84_Met_CD45_pos_F04_S928_comb !Sample_geo_accession = GSM3199356 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449971 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199357 !Sample_title = cy79_p5_CD45_pos_PD1_pos_G06_S654_comb !Sample_geo_accession = GSM3199357 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449970 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199358 !Sample_title = CY94CD45POS_1_D04_S136_comb !Sample_geo_accession = GSM3199358 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449969 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199359 !Sample_title = Cy72_CD45_A09_S681_comb !Sample_geo_accession = GSM3199359 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449968 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199360 !Sample_title = cy80_Cd45_pos_PD1_pos_C04_S64_comb !Sample_geo_accession = GSM3199360 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449967 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199361 !Sample_title = cy80_CD45_pos_PD1_pos_D05_S137_comb !Sample_geo_accession = GSM3199361 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450040 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199362 !Sample_title = cy60_1_cd_45_pos_3_H02_S374_comb !Sample_geo_accession = GSM3199362 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450039 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199363 !Sample_title = cy80_Cd45_pos_PD1_pos_C01_S61_comb !Sample_geo_accession = GSM3199363 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450038 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199364 !Sample_title = cy53_1_CD45_pos_1_F03_S63_comb !Sample_geo_accession = GSM3199364 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450037 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199365 !Sample_title = cy80_CD45_neg_C01_S409_comb !Sample_geo_accession = GSM3199365 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450036 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199366 !Sample_title = cy74_CD45_pos_E10_S730_comb !Sample_geo_accession = GSM3199366 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450035 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199367 !Sample_title = cy79_p5_CD45_pos_PD1_pos_G11_S659_comb !Sample_geo_accession = GSM3199367 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450034 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199368 !Sample_title = CY89A_Core_15_H08_S92_comb !Sample_geo_accession = GSM3199368 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450033 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199369 !Sample_title = cy80_Cd45_pos_PD1_pos_B02_S38_comb !Sample_geo_accession = GSM3199369 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450032 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199370 !Sample_title = cy53_1_CD45_pos_1_F09_S69_comb !Sample_geo_accession = GSM3199370 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450031 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199371 !Sample_title = cy80_CD45_pos_PD1_pos_E12_S156_comb !Sample_geo_accession = GSM3199371 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450030 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199372 !Sample_title = cy79_p5_CD45_pos_PD1_pos_H11_S671_comb !Sample_geo_accession = GSM3199372 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450029 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199373 !Sample_title = cy80_Cd45_pos_PD1_pos_C05_S65_comb !Sample_geo_accession = GSM3199373 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450028 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199374 !Sample_title = Cy74_CD45_H09_S477_comb !Sample_geo_accession = GSM3199374 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450027 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199375 !Sample_title = cy60_1_cd_45_pos_3_C11_S323_comb !Sample_geo_accession = GSM3199375 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450026 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199376 !Sample_title = cy60_1_cd_45_pos_3_F02_S350_comb !Sample_geo_accession = GSM3199376 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450025 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199377 !Sample_title = CY58_1_CD45_C10_S994_comb !Sample_geo_accession = GSM3199377 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450024 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199378 !Sample_title = CY58_1_CD45_G04_S1036_comb !Sample_geo_accession = GSM3199378 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450023 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199379 !Sample_title = cy72_CD45_pos_C09_S897_comb !Sample_geo_accession = GSM3199379 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450022 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199380 !Sample_title = cy58_1_CD45_pos_A08_S584_comb !Sample_geo_accession = GSM3199380 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450021 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199381 !Sample_title = cy80_CD45_neg_F07_S451_comb !Sample_geo_accession = GSM3199381 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450020 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199382 !Sample_title = CY88CD45POS_2_G04_S460_comb !Sample_geo_accession = GSM3199382 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450019 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199383 !Sample_title = cy58_1_CD45_pos_B01_S589_comb !Sample_geo_accession = GSM3199383 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450018 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199384 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_F02_S446_comb !Sample_geo_accession = GSM3199384 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449916 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199385 !Sample_title = cy94_cd45pos_4_E05_S53_comb !Sample_geo_accession = GSM3199385 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449915 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199386 !Sample_title = cy74_CD45_pos_G01_S745_comb !Sample_geo_accession = GSM3199386 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449914 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199387 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_G07_S463_comb !Sample_geo_accession = GSM3199387 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449913 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199388 !Sample_title = cy58_1_CD45_pos_E06_S630_comb !Sample_geo_accession = GSM3199388 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449912 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199389 !Sample_title = cy80_CD45_pos_PD1_pos_C10_S130_comb !Sample_geo_accession = GSM3199389 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449911 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199390 !Sample_title = CY88CD45POS_2_B05_S401_comb !Sample_geo_accession = GSM3199390 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449910 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199391 !Sample_title = CY89COREQ1_D12_S336_comb !Sample_geo_accession = GSM3199391 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449909 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199392 !Sample_title = Cy71_CD45_E01_S529_comb !Sample_geo_accession = GSM3199392 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449908 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199393 !Sample_title = cy60_1_cd_45_pos_4_G08_S80_comb !Sample_geo_accession = GSM3199393 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449907 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199394 !Sample_title = cy84_Primary_CD45_pos_C10_S418_comb !Sample_geo_accession = GSM3199394 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449906 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199395 !Sample_title = cy94_cd45pos_4_A05_S5_comb !Sample_geo_accession = GSM3199395 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449905 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199396 !Sample_title = cy94_cd45pos_A08_S104_comb !Sample_geo_accession = GSM3199396 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449904 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199397 !Sample_title = cy88_cd_45_pos_3_F04_S640_comb !Sample_geo_accession = GSM3199397 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449903 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199398 !Sample_title = CY89A_Core_15_D09_S45_comb !Sample_geo_accession = GSM3199398 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449902 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199399 !Sample_title = CY89COREQ1_D02_S326_comb !Sample_geo_accession = GSM3199399 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449901 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199400 !Sample_title = Cy71_CD45_C06_S510_comb !Sample_geo_accession = GSM3199400 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449900 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199401 !Sample_title = CY75_1FNA_H11_S575_comb !Sample_geo_accession = GSM3199401 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449899 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199402 !Sample_title = CY89FNA_B08_S212_comb !Sample_geo_accession = GSM3199402 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449898 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199403 !Sample_title = cy72_CD45_pos_A10_S874_comb !Sample_geo_accession = GSM3199403 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449897 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199404 !Sample_title = Cy81_FNA_CD45_D04_S232_comb !Sample_geo_accession = GSM3199404 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449896 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199405 !Sample_title = cy80_CD45_pos_PD1_pos_C02_S122_comb !Sample_geo_accession = GSM3199405 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449895 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199406 !Sample_title = cy53_1_CD45_pos_2_C06_S990_comb !Sample_geo_accession = GSM3199406 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449894 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199407 !Sample_title = CY89A_Core_15_D08_S44_comb !Sample_geo_accession = GSM3199407 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449893 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199408 !Sample_title = cy80_CD45_neg_D11_S431_comb !Sample_geo_accession = GSM3199408 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449966 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199409 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_B09_S405_comb !Sample_geo_accession = GSM3199409 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449965 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199410 !Sample_title = Cy81_FNA_CD45_G12_S276_comb !Sample_geo_accession = GSM3199410 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449964 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199411 !Sample_title = cy94_cd45pos_A02_S98_comb !Sample_geo_accession = GSM3199411 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449963 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199412 !Sample_title = CY94CD45POS_1_C10_S130_comb !Sample_geo_accession = GSM3199412 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449962 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199413 !Sample_title = cy80_Cd45_pos_PD1_pos_S181_H01_S181_comb !Sample_geo_accession = GSM3199413 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449961 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199414 !Sample_title = cy84_Met_CD45_pos_C07_S895_comb !Sample_geo_accession = GSM3199414 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449960 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199415 !Sample_title = cy94_cd45pos_B03_S111_comb !Sample_geo_accession = GSM3199415 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449813 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199416 !Sample_title = CY75_1FNA_F07_S547_comb_5 !Sample_geo_accession = GSM3199416 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449812 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199417 !Sample_title = cy79_p4_CD45_pos_PD1_pos_C03_S315_comb !Sample_geo_accession = GSM3199417 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449811 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199418 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_D01_S421_comb !Sample_geo_accession = GSM3199418 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449810 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199419 !Sample_title = CY89A_CD45_POS_10_H01_S277_comb !Sample_geo_accession = GSM3199419 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449809 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199420 !Sample_title = cy79_p5_CD45_pos_PD1_pos_C12_S612_comb !Sample_geo_accession = GSM3199420 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449807 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199421 !Sample_title = cy74_CD45_pos_C08_S704_comb !Sample_geo_accession = GSM3199421 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449806 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199422 !Sample_title = Cy74_CD45_H11_S479_comb !Sample_geo_accession = GSM3199422 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449805 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199423 !Sample_title = cy94_cd45pos_4_E06_S54_comb !Sample_geo_accession = GSM3199423 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449804 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199424 !Sample_title = CY88CD45POS_2_F03_S447_comb !Sample_geo_accession = GSM3199424 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449803 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199425 !Sample_title = Cy74_CD45_D02_S422_comb !Sample_geo_accession = GSM3199425 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449808 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199426 !Sample_title = cy84_Primary_CD45_pos_B12_S408_comb !Sample_geo_accession = GSM3199426 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449802 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199427 !Sample_title = Cy81_FNA_CD45_A04_S196_comb !Sample_geo_accession = GSM3199427 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449801 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199428 !Sample_title = cy79_p4_CD45_pos_PD1_pos_H05_S377_comb !Sample_geo_accession = GSM3199428 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449800 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199429 !Sample_title = cy80_Cd45_pos_PD1_pos_S113_E05_S113_comb !Sample_geo_accession = GSM3199429 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449799 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199430 !Sample_title = cy79_p5_CD45_pos_PD1_pos_H12_S672_comb !Sample_geo_accession = GSM3199430 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449798 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199431 !Sample_title = cy74_CD45_pos_F08_S740_comb !Sample_geo_accession = GSM3199431 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449797 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199432 !Sample_title = cy94_cd45neg_cd90pos_B02_S302_comb !Sample_geo_accession = GSM3199432 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449929 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199433 !Sample_title = cy94_cd45pos_D09_S141_comb !Sample_geo_accession = GSM3199433 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449928 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199434 !Sample_title = cy53_1_CD45_pos_2_H02_S1046_comb !Sample_geo_accession = GSM3199434 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449927 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199435 !Sample_title = cy94_cd45pos_4_H10_S94_comb !Sample_geo_accession = GSM3199435 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449926 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199436 !Sample_title = CY75_1FNA_A05_S485_comb_6 !Sample_geo_accession = GSM3199436 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449925 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199437 !Sample_title = Cy81_FNA_CD45_B05_S209_comb !Sample_geo_accession = GSM3199437 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449924 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199438 !Sample_title = cy74_CD45_pos_C06_S702_comb !Sample_geo_accession = GSM3199438 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449923 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199439 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_C03_S219_comb !Sample_geo_accession = GSM3199439 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449922 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199440 !Sample_title = CY88CD45POS_2_C09_S417_comb !Sample_geo_accession = GSM3199440 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449921 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199441 !Sample_title = cy72_CD45_pos_B12_S888_comb !Sample_geo_accession = GSM3199441 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449920 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199442 !Sample_title = cy79_p4_CD45_pos_PD1_pos_B12_S312_comb !Sample_geo_accession = GSM3199442 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449919 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199443 !Sample_title = cy74_CD45_pos_B07_S691_comb !Sample_geo_accession = GSM3199443 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449918 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199444 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_C09_S225_comb !Sample_geo_accession = GSM3199444 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449917 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199445 !Sample_title = cy58_1_CD45_pos_F05_S641_comb !Sample_geo_accession = GSM3199445 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450003 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199446 !Sample_title = cy80_Cd45_pos_PD1_pos_B04_S40_comb !Sample_geo_accession = GSM3199446 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450002 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199447 !Sample_title = Cy72_CD45_G02_S746_comb !Sample_geo_accession = GSM3199447 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450001 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199448 !Sample_title = CY88CD45POS_7_C09_S225_comb !Sample_geo_accession = GSM3199448 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450000 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199449 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_E10_S538_comb !Sample_geo_accession = GSM3199449 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449999 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199450 !Sample_title = CY88CD45POS_7_D07_S235_comb !Sample_geo_accession = GSM3199450 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449998 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199451 !Sample_title = cy60_1_cd_45_pos_4_H09_S93_comb !Sample_geo_accession = GSM3199451 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449997 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199452 !Sample_title = cy79_p4_CD45_pos_PD1_pos_A11_S299_comb !Sample_geo_accession = GSM3199452 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449996 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199453 !Sample_title = cy94_cd45neg_cd90pos_G08_S368_comb !Sample_geo_accession = GSM3199453 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449995 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199454 !Sample_title = CY58_1_CD45_H01_S1045_comb !Sample_geo_accession = GSM3199454 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449988 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199455 !Sample_title = cy79_p5_CD45_pos_PD1_pos_G09_S657_comb !Sample_geo_accession = GSM3199455 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449994 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199456 !Sample_title = CY75_1FNA_E03_S531_comb !Sample_geo_accession = GSM3199456 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449993 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199457 !Sample_title = cy72_CD45_pos_H03_S951_comb !Sample_geo_accession = GSM3199457 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449992 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199458 !Sample_title = CY75_1FNA_F10_S550_comb !Sample_geo_accession = GSM3199458 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449991 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199459 !Sample_title = CY89FNA_F07_S259_comb !Sample_geo_accession = GSM3199459 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449990 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199460 !Sample_title = cy79_p5_CD45_pos_PD1_pos_C06_S606_comb !Sample_geo_accession = GSM3199460 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449989 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199461 !Sample_title = CY88CD45POS_2_G09_S465_comb !Sample_geo_accession = GSM3199461 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449987 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199462 !Sample_title = cy80_Cd45_pos_PD1_pos_S139_F07_S139_comb !Sample_geo_accession = GSM3199462 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449986 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199463 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_G05_S269_comb !Sample_geo_accession = GSM3199463 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449985 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199464 !Sample_title = cy80_Cd45_pos_PD1_pos_D08_S92_comb !Sample_geo_accession = GSM3199464 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449984 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199465 !Sample_title = cy80_CD45_neg_D09_S429_comb !Sample_geo_accession = GSM3199465 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449983 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199466 !Sample_title = cy80_Cd45_pos_PD1_pos_D07_S91_comb !Sample_geo_accession = GSM3199466 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449982 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199467 !Sample_title = cy79_p4_CD45_pos_PD1_pos_B08_S308_comb !Sample_geo_accession = GSM3199467 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449981 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199468 !Sample_title = CY89A_CD45_POS_10_G11_S275_comb !Sample_geo_accession = GSM3199468 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449980 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199469 !Sample_title = cy53_1_CD45_pos_2_E05_S1013_comb !Sample_geo_accession = GSM3199469 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449979 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199470 !Sample_title = cy82_CD45_pos_2_A10_S490_comb !Sample_geo_accession = GSM3199470 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449978 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199471 !Sample_title = cy94_cd45pos_4_A10_S10_comb !Sample_geo_accession = GSM3199471 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449977 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199472 !Sample_title = cy94_cd45pos_4_D10_S46_comb !Sample_geo_accession = GSM3199472 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449976 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199473 !Sample_title = cy94_cd45pos_4_D09_S45_comb !Sample_geo_accession = GSM3199473 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449975 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199474 !Sample_title = cy72_CD45_pos_G12_S948_comb !Sample_geo_accession = GSM3199474 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449974 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199475 !Sample_title = cy80_Cd45_pos_PD1_pos_S111_E03_S111_comb !Sample_geo_accession = GSM3199475 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449667 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199476 !Sample_title = cy60_1_cd_45_pos_3_D11_S335_comb !Sample_geo_accession = GSM3199476 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449666 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199477 !Sample_title = cy60_1_cd_45_pos_3_C08_S320_comb !Sample_geo_accession = GSM3199477 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449665 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199478 !Sample_title = CY58_1_CD45_B12_S984_comb !Sample_geo_accession = GSM3199478 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449616 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199479 !Sample_title = CY75_1FNA_F04_S544_comb !Sample_geo_accession = GSM3199479 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449614 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199480 !Sample_title = CY89A_CD45_POS_10_G09_S273_comb !Sample_geo_accession = GSM3199480 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449613 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199481 !Sample_title = cy80_CD45_pos_PD1_pos_G12_S180_comb !Sample_geo_accession = GSM3199481 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449612 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199482 !Sample_title = cy84_Met_CD45_pos_G07_S943_comb !Sample_geo_accession = GSM3199482 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449611 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199483 !Sample_title = CY89A_Core_15_B11_S23_comb !Sample_geo_accession = GSM3199483 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449610 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199484 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_B05_S209_comb !Sample_geo_accession = GSM3199484 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449609 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199485 !Sample_title = CY75_1FNA_F06_S546_comb !Sample_geo_accession = GSM3199485 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449615 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199486 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_B05_S401_comb !Sample_geo_accession = GSM3199486 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449608 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199487 !Sample_title = cy72_CD45_pos_D06_S906_comb !Sample_geo_accession = GSM3199487 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449607 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199488 !Sample_title = cy53_1_CD45_pos_2_B02_S974_comb !Sample_geo_accession = GSM3199488 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449606 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199489 !Sample_title = cy79_p5_CD45_pos_PD1_pos_E05_S629_comb !Sample_geo_accession = GSM3199489 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449605 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199490 !Sample_title = cy94_cd45pos_F10_S166_comb !Sample_geo_accession = GSM3199490 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449604 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199491 !Sample_title = Cy74_CD45_C01_S409_comb !Sample_geo_accession = GSM3199491 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449603 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199492 !Sample_title = CY58_1_CD45_B03_S975_comb !Sample_geo_accession = GSM3199492 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449602 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199493 !Sample_title = cy84_Primary_CD45_pos_G10_S466_comb !Sample_geo_accession = GSM3199493 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449601 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199494 !Sample_title = CY84_PRIM_POS_ALL_6_C04_S412_comb !Sample_geo_accession = GSM3199494 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449600 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199495 !Sample_title = cy79_p3_CD45_pos_PD1_neg_A07_S103_comb !Sample_geo_accession = GSM3199495 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449721 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199496 !Sample_title = cy53_1_CD45_pos_1_A04_S4_comb !Sample_geo_accession = GSM3199496 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449720 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199497 !Sample_title = cy74_CD45_pos_C10_S706_comb !Sample_geo_accession = GSM3199497 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449719 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199498 !Sample_title = cy79_p4_CD45_pos_PD1_pos_B01_S301_comb !Sample_geo_accession = GSM3199498 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449718 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199499 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_F11_S455_comb !Sample_geo_accession = GSM3199499 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449717 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199500 !Sample_title = cy94_cd45pos_4_B11_S23_comb !Sample_geo_accession = GSM3199500 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449716 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199501 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_C06_S222_comb !Sample_geo_accession = GSM3199501 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449715 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199502 !Sample_title = cy79_p5_CD45_pos_PD1_pos_F07_S643_comb !Sample_geo_accession = GSM3199502 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449714 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199503 !Sample_title = cy79_p5_CD45_pos_PD1_pos_F01_S637_comb !Sample_geo_accession = GSM3199503 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449713 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199504 !Sample_title = cy53_1_CD45_pos_1_F12_S72_comb !Sample_geo_accession = GSM3199504 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449712 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199505 !Sample_title = cy72_CD45_pos_B08_S884_comb !Sample_geo_accession = GSM3199505 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449763 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199506 !Sample_title = cy88_cd_45_pos_C02_S410_comb !Sample_geo_accession = GSM3199506 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449762 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199507 !Sample_title = cy53_1_CD45_pos_1_C02_S26_comb !Sample_geo_accession = GSM3199507 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449761 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199508 !Sample_title = CY75_1FNA_F12_S552_comb !Sample_geo_accession = GSM3199508 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449760 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199509 !Sample_title = cy53_1_CD45_pos_1_H10_S94_comb !Sample_geo_accession = GSM3199509 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449759 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199510 !Sample_title = cy74_CD45_pos_F10_S742_comb !Sample_geo_accession = GSM3199510 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449758 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199511 !Sample_title = Cy74_CD45_D06_S426_comb !Sample_geo_accession = GSM3199511 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449755 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199512 !Sample_title = CY94CD45POS_1_B04_S112_comb !Sample_geo_accession = GSM3199512 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449754 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199513 !Sample_title = cy79_p3_CD45_pos_PD1_neg_E06_S150_comb !Sample_geo_accession = GSM3199513 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449753 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199514 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_E09_S441_comb !Sample_geo_accession = GSM3199514 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449752 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199515 !Sample_title = Cy72_CD45_C05_S701_comb !Sample_geo_accession = GSM3199515 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449757 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199516 !Sample_title = cy80_CD45_pos_PD1_pos_D04_S136_comb !Sample_geo_accession = GSM3199516 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449756 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199517 !Sample_title = CY89A_CD45_POS_6_E04_S148_comb !Sample_geo_accession = GSM3199517 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449751 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199518 !Sample_title = cy74_CD45_pos_G05_S749_comb !Sample_geo_accession = GSM3199518 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449750 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199519 !Sample_title = cy58_1_CD45_pos_A06_S582_comb !Sample_geo_accession = GSM3199519 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449749 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199520 !Sample_title = cy79_p5_CD45_pos_PD1_pos_B06_S594_comb !Sample_geo_accession = GSM3199520 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449748 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199521 !Sample_title = cy79_p4_CD45_pos_PD1_pos_E10_S346_comb !Sample_geo_accession = GSM3199521 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449747 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199522 !Sample_title = CY75_1FNA_A12_S492_comb !Sample_geo_accession = GSM3199522 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449746 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199523 !Sample_title = cy84_Primary_CD45_pos_H03_S471_comb !Sample_geo_accession = GSM3199523 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449745 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199524 !Sample_title = Cy74_CD45_C04_S412_comb !Sample_geo_accession = GSM3199524 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449744 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199525 !Sample_title = cy94_cd45pos_H06_S186_comb !Sample_geo_accession = GSM3199525 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449743 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199526 !Sample_title = cy60_1_cd_45_pos_3_G11_S371_comb !Sample_geo_accession = GSM3199526 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449742 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199527 !Sample_title = CY89A_CD45_POS_6_G05_S173_comb !Sample_geo_accession = GSM3199527 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449741 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199528 !Sample_title = cy82_CD45_pos_2_A02_S482_comb !Sample_geo_accession = GSM3199528 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449740 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199529 !Sample_title = CY88CD45_150813_H11_S383_comb !Sample_geo_accession = GSM3199529 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449739 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199530 !Sample_title = Cy74_CD45_G06_S462_comb !Sample_geo_accession = GSM3199530 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449738 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199531 !Sample_title = CY58_1_CD45_B04_S976_comb !Sample_geo_accession = GSM3199531 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449737 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199532 !Sample_title = CY58_1_CD45_F07_S1027_comb !Sample_geo_accession = GSM3199532 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449736 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199533 !Sample_title = cy79_p5_CD45_pos_PD1_pos_F11_S647_comb !Sample_geo_accession = GSM3199533 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449735 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199534 !Sample_title = cy58_1_CD45_pos_C03_S603_comb !Sample_geo_accession = GSM3199534 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449734 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199535 !Sample_title = cy79_p4_CD45_pos_PD1_pos_B09_S309_comb !Sample_geo_accession = GSM3199535 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449711 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199536 !Sample_title = cy80_Cd45_pos_PD1_pos_S164_G08_S164_comb !Sample_geo_accession = GSM3199536 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449710 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199537 !Sample_title = cy80_Cd45_pos_Pd1_neg_S347_G11_S347_comb !Sample_geo_accession = GSM3199537 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449709 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199538 !Sample_title = Cy74_CD45_E09_S441_comb !Sample_geo_accession = GSM3199538 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449708 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199539 !Sample_title = CY89A_CD45_POS_6_A12_S108_comb !Sample_geo_accession = GSM3199539 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449707 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199540 !Sample_title = cy53_1_CD45_pos_1_A10_S10_comb !Sample_geo_accession = GSM3199540 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449706 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199541 !Sample_title = cy80_Cd45_pos_Pd1_neg_S249_C09_S249_comb !Sample_geo_accession = GSM3199541 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449705 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199542 !Sample_title = cy80_Cd45_pos_PD1_pos_A11_S23_comb !Sample_geo_accession = GSM3199542 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449704 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199543 !Sample_title = cy60_1_cd_45_pos_3_F11_S359_comb !Sample_geo_accession = GSM3199543 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449703 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199544 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_A12_S396_comb !Sample_geo_accession = GSM3199544 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449702 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199545 !Sample_title = cy80_CD45_neg_A03_S387_comb !Sample_geo_accession = GSM3199545 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449701 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199546 !Sample_title = cy84_Met_CD45_pos_G09_S945_comb !Sample_geo_accession = GSM3199546 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449700 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199547 !Sample_title = cy79_p5_CD45_pos_PD1_pos_E11_S635_comb !Sample_geo_accession = GSM3199547 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449699 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199548 !Sample_title = cy80_CD45_pos_PD1_pos_F08_S164_comb !Sample_geo_accession = GSM3199548 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449698 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199549 !Sample_title = cy72_CD45_pos_C03_S891_comb !Sample_geo_accession = GSM3199549 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449697 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199550 !Sample_title = cy79_p4_CD45_pos_PD1_pos_C07_S319_comb !Sample_geo_accession = GSM3199550 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449696 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199551 !Sample_title = cy79_p4_CD45_pos_PD1_pos_B04_S304_comb !Sample_geo_accession = GSM3199551 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449695 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199552 !Sample_title = CY89COREQ1_E12_S348_comb !Sample_geo_accession = GSM3199552 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449694 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199553 !Sample_title = cy53_1_CD45_pos_1_G05_S77_comb !Sample_geo_accession = GSM3199553 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449693 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199554 !Sample_title = cy79_p4_CD45_pos_PD1_pos_G09_S369_comb !Sample_geo_accession = GSM3199554 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449692 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199555 !Sample_title = cy72_CD45_pos_B05_S881_comb !Sample_geo_accession = GSM3199555 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449691 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199556 !Sample_title = CY94CD45POS_1_B03_S111_comb !Sample_geo_accession = GSM3199556 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449690 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199557 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_D06_S234_comb !Sample_geo_accession = GSM3199557 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449689 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199558 !Sample_title = cy80_Cd45_pos_Pd1_neg_S217_B01_S217_comb !Sample_geo_accession = GSM3199558 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449688 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199559 !Sample_title = cy79_p4_CD45_pos_PD1_pos_B02_S302_comb !Sample_geo_accession = GSM3199559 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449687 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199560 !Sample_title = cy74_CD45_pos_D04_S712_comb !Sample_geo_accession = GSM3199560 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449686 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199561 !Sample_title = cy58_1_CD45_pos_G08_S656_comb !Sample_geo_accession = GSM3199561 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449685 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199562 !Sample_title = cy58_1_CD45_pos_G12_S660_comb !Sample_geo_accession = GSM3199562 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449684 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199563 !Sample_title = CY89COREQ1_G05_S365_comb !Sample_geo_accession = GSM3199563 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449765 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199564 !Sample_title = cy74_CD45_pos_A03_S675_comb !Sample_geo_accession = GSM3199564 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449764 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199565 !Sample_title = cy72_CD45_pos_C01_S889_comb !Sample_geo_accession = GSM3199565 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449733 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199566 !Sample_title = cy88_cd_45_pos_3_B11_S599_comb !Sample_geo_accession = GSM3199566 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449732 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199567 !Sample_title = cy88_cd_45_pos_G11_S467_comb !Sample_geo_accession = GSM3199567 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449731 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199568 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_F07_S259_comb !Sample_geo_accession = GSM3199568 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449730 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199569 !Sample_title = CY75_1FNA_H03_S567_comb !Sample_geo_accession = GSM3199569 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449729 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199570 !Sample_title = cy80_CD45_pos_PD1_pos_D03_S135_comb !Sample_geo_accession = GSM3199570 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449727 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199571 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_G12_S276_comb !Sample_geo_accession = GSM3199571 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449726 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199572 !Sample_title = cy60_1_cd_45_pos_4_B09_S21_comb !Sample_geo_accession = GSM3199572 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449577 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199573 !Sample_title = cy60_1_cd_45_pos_3_D06_S330_comb !Sample_geo_accession = GSM3199573 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449576 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199574 !Sample_title = CY84_PRIM_POS_ALL_8_B08_S116_comb !Sample_geo_accession = GSM3199574 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449575 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199575 !Sample_title = cy84_Met_CD45_pos_A04_S868_comb !Sample_geo_accession = GSM3199575 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449574 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199576 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_C09_S417_comb !Sample_geo_accession = GSM3199576 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449573 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199577 !Sample_title = CY89A_CD45_POS_10_E11_S251_comb !Sample_geo_accession = GSM3199577 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449572 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199578 !Sample_title = cy80_Cd45_pos_Pd1_neg_S344_G08_S344_comb !Sample_geo_accession = GSM3199578 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449571 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199579 !Sample_title = CY88CD45_150813_B05_S305_comb_7 !Sample_geo_accession = GSM3199579 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449570 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199580 !Sample_title = cy79_p4_CD45_pos_PD1_pos_G10_S370_comb !Sample_geo_accession = GSM3199580 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449569 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199581 !Sample_title = cy84_Primary_CD45_pos_D09_S429_comb !Sample_geo_accession = GSM3199581 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449568 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199582 !Sample_title = CY94CD45POS_1_G01_S169_comb !Sample_geo_accession = GSM3199582 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449567 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199583 !Sample_title = cy80_CD45_pos_PD1_pos_H02_S182_comb !Sample_geo_accession = GSM3199583 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449566 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199584 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_D12_S1008_comb !Sample_geo_accession = GSM3199584 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449565 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199585 !Sample_title = cy88_cd_45_pos_3_H08_S668_comb !Sample_geo_accession = GSM3199585 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449564 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199586 !Sample_title = Cy81_FNA_CD45_C04_S220_comb !Sample_geo_accession = GSM3199586 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449683 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199587 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_A02_S194_comb !Sample_geo_accession = GSM3199587 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449682 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199588 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_C12_S420_comb !Sample_geo_accession = GSM3199588 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449681 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199589 !Sample_title = cy79_p5_CD45_pos_PD1_pos_H03_S663_comb !Sample_geo_accession = GSM3199589 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449680 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199590 !Sample_title = cy58_1_CD45_pos_H03_S663_comb !Sample_geo_accession = GSM3199590 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449679 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199591 !Sample_title = cy84_Met_CD45_pos_A11_S875_comb !Sample_geo_accession = GSM3199591 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449678 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199592 !Sample_title = cy82_CD45_pos_1_E06_S534_comb !Sample_geo_accession = GSM3199592 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449677 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199593 !Sample_title = CY89A_Core_15_B07_S19_comb !Sample_geo_accession = GSM3199593 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449676 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199594 !Sample_title = cy80_CD45_pos_PD1_pos_G09_S177_comb !Sample_geo_accession = GSM3199594 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449675 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199595 !Sample_title = cy94_cd45pos_C05_S125_comb !Sample_geo_accession = GSM3199595 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449674 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199596 !Sample_title = CY75_1FNA_G03_S555_comb !Sample_geo_accession = GSM3199596 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449673 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199597 !Sample_title = CY89A_Core_15_A06_S6_comb !Sample_geo_accession = GSM3199597 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449672 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199598 !Sample_title = cy82_CD45_pos_1_B09_S501_comb !Sample_geo_accession = GSM3199598 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449671 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199599 !Sample_title = cy88_cd_45_pos_D02_S422_comb !Sample_geo_accession = GSM3199599 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449670 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199600 !Sample_title = cy80_CD45_pos_PD1_pos_D06_S138_comb !Sample_geo_accession = GSM3199600 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449669 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199601 !Sample_title = cy79_p4_CD45_pos_PD1_pos_H09_S381_comb !Sample_geo_accession = GSM3199601 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449668 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199602 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_F07_S451_comb !Sample_geo_accession = GSM3199602 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449725 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199603 !Sample_title = cy84_Primary_CD45_pos_G07_S463_comb !Sample_geo_accession = GSM3199603 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449724 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199604 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_F11_S263_comb !Sample_geo_accession = GSM3199604 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449792 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199605 !Sample_title = cy80_Cd45_pos_PD1_pos_C09_S69_comb !Sample_geo_accession = GSM3199605 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449723 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199606 !Sample_title = CY89COREQ1_D07_S331_comb !Sample_geo_accession = GSM3199606 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449796 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199607 !Sample_title = cy80_Cd45_pos_PD1_pos_A12_S24_comb !Sample_geo_accession = GSM3199607 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449795 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199608 !Sample_title = Cy74_CD45_F06_S450_comb !Sample_geo_accession = GSM3199608 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449794 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199609 !Sample_title = CY88CD45POS_2_F11_S455_comb !Sample_geo_accession = GSM3199609 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449793 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199610 !Sample_title = cy58_1_CD45_pos_A03_S579_comb !Sample_geo_accession = GSM3199610 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449791 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199611 !Sample_title = cy58_1_CD45_pos_D09_S621_comb !Sample_geo_accession = GSM3199611 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449790 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199612 !Sample_title = Cy71_CD45_G07_S559_comb !Sample_geo_accession = GSM3199612 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449789 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199613 !Sample_title = cy80_Cd45_pos_Pd1_neg_S242_C02_S242_comb !Sample_geo_accession = GSM3199613 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449788 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199614 !Sample_title = cy88_cd_45_pos_3_B03_S591_comb !Sample_geo_accession = GSM3199614 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449787 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199615 !Sample_title = cy58_1_CD45_pos_B06_S594_comb !Sample_geo_accession = GSM3199615 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449786 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199616 !Sample_title = cy94_cd45pos_F08_S164_comb !Sample_geo_accession = GSM3199616 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449785 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199617 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_E11_S443_comb !Sample_geo_accession = GSM3199617 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449784 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199618 !Sample_title = cy82_CD45_pos_2_F01_S541_comb !Sample_geo_accession = GSM3199618 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449783 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199619 !Sample_title = cy94_cd45pos_4_B07_S19_comb !Sample_geo_accession = GSM3199619 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449782 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199620 !Sample_title = cy84_Primary_CD45_pos_D03_S423_comb !Sample_geo_accession = GSM3199620 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449781 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199621 !Sample_title = cy94_cd45pos_4_A03_S3_comb !Sample_geo_accession = GSM3199621 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449780 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199622 !Sample_title = cy60_1_cd_45_pos_4_C04_S28_comb !Sample_geo_accession = GSM3199622 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449779 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199623 !Sample_title = CY88CD45_150813_F09_S357_comb !Sample_geo_accession = GSM3199623 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449778 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199624 !Sample_title = CY89COREQ1_C10_S322_comb !Sample_geo_accession = GSM3199624 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449233 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199625 !Sample_title = cy80_Cd45_pos_PD1_pos_S109_E01_S109_comb !Sample_geo_accession = GSM3199625 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449232 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199626 !Sample_title = cy58_1_CD45_pos_C07_S607_comb !Sample_geo_accession = GSM3199626 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449231 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199627 !Sample_title = cy80_Cd45_pos_Pd1_neg_S268_D04_S268_comb !Sample_geo_accession = GSM3199627 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449230 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199628 !Sample_title = CY75_1FNA_E09_S537_comb !Sample_geo_accession = GSM3199628 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449229 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199629 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_F08_S260_comb !Sample_geo_accession = GSM3199629 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449228 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199630 !Sample_title = cy94_cd45pos_C02_S122_comb !Sample_geo_accession = GSM3199630 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449227 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199631 !Sample_title = cy72_CD45_pos_F11_S935_comb !Sample_geo_accession = GSM3199631 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449226 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199632 !Sample_title = CY89A_CD45_POS_6_H02_S182_comb !Sample_geo_accession = GSM3199632 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449225 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199633 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_C01_S505_comb !Sample_geo_accession = GSM3199633 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449224 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199634 !Sample_title = cy79_p4_CD45_pos_PD1_pos_C05_S317_comb !Sample_geo_accession = GSM3199634 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449219 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199635 !Sample_title = CY94CD45POS_1_C08_S128_comb !Sample_geo_accession = GSM3199635 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449223 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199636 !Sample_title = cy74_CD45_pos_F04_S736_comb !Sample_geo_accession = GSM3199636 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449222 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199637 !Sample_title = cy60_1_cd_45_pos_3_H05_S377_comb !Sample_geo_accession = GSM3199637 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449221 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199638 !Sample_title = cy79_p5_CD45_pos_PD1_pos_C08_S608_comb !Sample_geo_accession = GSM3199638 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449220 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199639 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_D11_S527_comb !Sample_geo_accession = GSM3199639 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449218 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199640 !Sample_title = CY88CD45_150813_B09_S309_comb !Sample_geo_accession = GSM3199640 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449217 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199641 !Sample_title = cy82_CD45_pos_1_A07_S487_comb !Sample_geo_accession = GSM3199641 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449216 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199642 !Sample_title = CY89A_CD45_POS_6_H01_S181_comb !Sample_geo_accession = GSM3199642 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449215 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199643 !Sample_title = CY89A_Core_15_F07_S67_comb !Sample_geo_accession = GSM3199643 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449214 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199644 !Sample_title = CY89A_CD45_POS_6_F09_S165_comb !Sample_geo_accession = GSM3199644 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449213 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199645 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_B07_S211_comb !Sample_geo_accession = GSM3199645 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449212 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199646 !Sample_title = CY88CD45POS_2_D09_S429_comb !Sample_geo_accession = GSM3199646 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449211 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199647 !Sample_title = CY94CD45POS_1_C09_S129_comb !Sample_geo_accession = GSM3199647 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449210 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199648 !Sample_title = cy80_CD45_pos_PD1_pos_B06_S114_comb !Sample_geo_accession = GSM3199648 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449281 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199649 !Sample_title = cy53_1_CD45_pos_1_H01_S85_comb !Sample_geo_accession = GSM3199649 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449280 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199650 !Sample_title = cy74_CD45_pos_D08_S716_comb !Sample_geo_accession = GSM3199650 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449279 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199651 !Sample_title = CY88CD45POS_7_B05_S209_comb !Sample_geo_accession = GSM3199651 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449278 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199652 !Sample_title = CY88CD45_150813_H10_S382_comb !Sample_geo_accession = GSM3199652 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449277 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199653 !Sample_title = cy74_CD45_pos_A02_S674_comb !Sample_geo_accession = GSM3199653 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449276 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199654 !Sample_title = Cy74_CD45_C09_S417_comb !Sample_geo_accession = GSM3199654 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449275 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199655 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_G10_S274_comb !Sample_geo_accession = GSM3199655 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449274 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199656 !Sample_title = CY88CD45POS_2_F05_S449_comb !Sample_geo_accession = GSM3199656 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449273 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199657 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_C12_S228_comb !Sample_geo_accession = GSM3199657 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449272 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199658 !Sample_title = CY89A_CD45_POS_6_C09_S129_comb !Sample_geo_accession = GSM3199658 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449271 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199659 !Sample_title = cy72_CD45_pos_E12_S924_comb !Sample_geo_accession = GSM3199659 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449270 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199660 !Sample_title = CY75_1FNA_G01_S553_comb !Sample_geo_accession = GSM3199660 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449269 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199661 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_E06_S438_comb !Sample_geo_accession = GSM3199661 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449268 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199662 !Sample_title = cy80_Cd45_pos_PD1_pos_C03_S63_comb !Sample_geo_accession = GSM3199662 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449265 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199663 !Sample_title = cy58_1_CD45_pos_G05_S653_comb !Sample_geo_accession = GSM3199663 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449264 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199664 !Sample_title = cy74_CD45_pos_A04_S676_comb !Sample_geo_accession = GSM3199664 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449263 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199665 !Sample_title = cy60_1_cd_45_pos_3_G05_S365_comb !Sample_geo_accession = GSM3199665 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449267 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199666 !Sample_title = CY88CD45POS_2_B01_S397_comb !Sample_geo_accession = GSM3199666 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449266 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199667 !Sample_title = Cy74_CD45_C08_S416_comb !Sample_geo_accession = GSM3199667 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449262 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199668 !Sample_title = cy58_1_CD45_pos_E09_S633_comb !Sample_geo_accession = GSM3199668 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449261 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199669 !Sample_title = cy72_CD45_pos_G08_S944_comb !Sample_geo_accession = GSM3199669 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449260 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199670 !Sample_title = cy80_Cd45_pos_PD1_pos_S143_F11_S143_comb !Sample_geo_accession = GSM3199670 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449259 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199671 !Sample_title = cy74_CD45_pos_F11_S743_comb !Sample_geo_accession = GSM3199671 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449258 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199672 !Sample_title = CY89A_Core_15_H09_S93_comb !Sample_geo_accession = GSM3199672 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449257 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199673 !Sample_title = Cy72_CD45_E12_S732_comb !Sample_geo_accession = GSM3199673 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449256 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199674 !Sample_title = cy80_CD45_pos_PD1_pos_H04_S184_comb !Sample_geo_accession = GSM3199674 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449255 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199675 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_E10_S442_comb !Sample_geo_accession = GSM3199675 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449254 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199676 !Sample_title = cy80_CD45_neg_G07_S463_comb !Sample_geo_accession = GSM3199676 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449253 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199677 !Sample_title = cy80_CD45_pos_PD1_pos_B04_S112_comb !Sample_geo_accession = GSM3199677 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449252 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199678 !Sample_title = cy80_CD45_pos_PD1_pos_A02_S98_comb !Sample_geo_accession = GSM3199678 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449251 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199679 !Sample_title = cy60_1_cd_45_pos_3_B10_S310_comb !Sample_geo_accession = GSM3199679 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449250 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199680 !Sample_title = CY89A_Core_15_A12_S12_comb !Sample_geo_accession = GSM3199680 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449249 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199681 !Sample_title = cy94_cd45pos_F02_S158_comb !Sample_geo_accession = GSM3199681 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449248 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199682 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_C11_S227_comb !Sample_geo_accession = GSM3199682 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449247 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199683 !Sample_title = cy88_cd_45_pos_C05_S413_comb !Sample_geo_accession = GSM3199683 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449193 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199684 !Sample_title = cy79_p5_CD45_pos_PD1_pos_C04_S604_comb !Sample_geo_accession = GSM3199684 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449192 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199685 !Sample_title = cy79_p3_CD45_pos_PD1_neg_A10_S106_comb !Sample_geo_accession = GSM3199685 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449191 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199686 !Sample_title = CY89COREQ1_G01_S361_comb !Sample_geo_accession = GSM3199686 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449190 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199687 !Sample_title = cy82_CD45_pos_1_E09_S537_comb !Sample_geo_accession = GSM3199687 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449189 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199688 !Sample_title = cy60_1_cd_45_pos_3_A01_S289_comb !Sample_geo_accession = GSM3199688 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449188 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199689 !Sample_title = cy74_CD45_pos_B03_S687_comb !Sample_geo_accession = GSM3199689 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449187 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199690 !Sample_title = cy74_CD45_pos_D05_S713_comb !Sample_geo_accession = GSM3199690 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449186 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199691 !Sample_title = cy94_cd45pos_H03_S183_comb !Sample_geo_accession = GSM3199691 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449184 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199692 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_F10_S1030_comb !Sample_geo_accession = GSM3199692 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449183 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199693 !Sample_title = CY58_1_CD45_C08_S992_comb !Sample_geo_accession = GSM3199693 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449182 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199694 !Sample_title = Cy74_CD45_E01_S433_comb !Sample_geo_accession = GSM3199694 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449181 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199695 !Sample_title = cy84_Primary_CD45_pos_G08_S464_comb !Sample_geo_accession = GSM3199695 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449185 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199696 !Sample_title = cy80_CD45_neg_H11_S479_comb !Sample_geo_accession = GSM3199696 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449180 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199697 !Sample_title = cy79_p4_CD45_pos_PD1_pos_D03_S327_comb !Sample_geo_accession = GSM3199697 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449179 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199698 !Sample_title = cy80_Cd45_pos_PD1_pos_C10_S70_comb !Sample_geo_accession = GSM3199698 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449178 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199699 !Sample_title = cy79_p5_CD45_pos_PD1_pos_C09_S609_comb !Sample_geo_accession = GSM3199699 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449177 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199700 !Sample_title = CY89A_CD45_POS_10_H06_S282_comb !Sample_geo_accession = GSM3199700 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449246 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199701 !Sample_title = cy79_p4_CD45_pos_PD1_pos_D11_S335_comb !Sample_geo_accession = GSM3199701 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449245 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199702 !Sample_title = CY89COREQ1_F04_S352_comb !Sample_geo_accession = GSM3199702 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449244 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199703 !Sample_title = cy53_1_CD45_pos_2_B10_S982_comb !Sample_geo_accession = GSM3199703 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449243 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199704 !Sample_title = cy58_1_CD45_pos_E11_S635_comb !Sample_geo_accession = GSM3199704 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449242 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199705 !Sample_title = cy80_Cd45_pos_Pd1_neg_S361_H01_S361_comb !Sample_geo_accession = GSM3199705 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449241 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199706 !Sample_title = cy80_Cd45_pos_PD1_pos_S116_E08_S116_comb !Sample_geo_accession = GSM3199706 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449240 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199707 !Sample_title = cy88_cd_45_pos_B01_S397_comb !Sample_geo_accession = GSM3199707 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449239 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199708 !Sample_title = cy79_p4_CD45_pos_PD1_pos_B05_S305_comb !Sample_geo_accession = GSM3199708 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449238 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199709 !Sample_title = cy80_CD45_pos_PD1_pos_H05_S185_comb !Sample_geo_accession = GSM3199709 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449237 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199710 !Sample_title = CY88CD45POS_7_B07_S211_comb !Sample_geo_accession = GSM3199710 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449236 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199711 !Sample_title = cy80_CD45_pos_PD1_pos_F09_S165_comb !Sample_geo_accession = GSM3199711 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449235 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199712 !Sample_title = Cy72_CD45_E05_S725_comb !Sample_geo_accession = GSM3199712 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449234 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199713 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_B11_S407_comb !Sample_geo_accession = GSM3199713 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449071 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199714 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_C05_S221_comb !Sample_geo_accession = GSM3199714 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449070 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199715 !Sample_title = cy53_1_CD45_pos_1_G01_S73_comb !Sample_geo_accession = GSM3199715 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449069 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199716 !Sample_title = cy72_CD45_pos_A09_S873_comb !Sample_geo_accession = GSM3199716 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449145 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199717 !Sample_title = cy80_Cd45_pos_PD1_pos_D10_S94_comb !Sample_geo_accession = GSM3199717 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449144 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199718 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_D09_S237_comb !Sample_geo_accession = GSM3199718 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449143 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199719 !Sample_title = CY89FNA_E01_S241_comb !Sample_geo_accession = GSM3199719 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449142 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199720 !Sample_title = cy80_CD45_pos_PD1_pos_A07_S103_comb !Sample_geo_accession = GSM3199720 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449141 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199721 !Sample_title = cy80_Cd45_pos_PD1_pos_S141_F09_S141_comb !Sample_geo_accession = GSM3199721 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449140 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199722 !Sample_title = cy53_1_CD45_pos_2_G04_S1036_comb !Sample_geo_accession = GSM3199722 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449139 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199723 !Sample_title = cy74_CD45_pos_C02_S698_comb !Sample_geo_accession = GSM3199723 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449138 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199724 !Sample_title = cy84_Primary_CD45_pos_D06_S426_comb !Sample_geo_accession = GSM3199724 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449132 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199725 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_C03_S411_comb !Sample_geo_accession = GSM3199725 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449137 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199726 !Sample_title = CY88CD45_150813_E08_S344_comb !Sample_geo_accession = GSM3199726 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449136 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199727 !Sample_title = cy94_cd45pos_H11_S191_comb !Sample_geo_accession = GSM3199727 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449135 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199728 !Sample_title = cy80_CD45_pos_PD1_pos_F10_S166_comb !Sample_geo_accession = GSM3199728 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449134 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199729 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_F11_S551_comb !Sample_geo_accession = GSM3199729 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449133 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199730 !Sample_title = CY89A_CD45_POS_10_E04_S244_comb !Sample_geo_accession = GSM3199730 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449131 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199731 !Sample_title = CY75_1FNA_G04_S556_comb !Sample_geo_accession = GSM3199731 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449130 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199732 !Sample_title = Cy74_CD45_B10_S406_comb !Sample_geo_accession = GSM3199732 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449129 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199733 !Sample_title = cy72_CD45_pos_D05_S905_comb !Sample_geo_accession = GSM3199733 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449128 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199734 !Sample_title = cy79_p4_CD45_pos_PD1_pos_F07_S355_comb !Sample_geo_accession = GSM3199734 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449127 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199735 !Sample_title = cy79_p5_CD45_pos_PD1_pos_H08_S668_comb !Sample_geo_accession = GSM3199735 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449126 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199736 !Sample_title = cy80_CD45_neg_B08_S404_comb !Sample_geo_accession = GSM3199736 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449125 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199737 !Sample_title = cy79_p5_CD45_pos_PD1_pos_H09_S669_comb !Sample_geo_accession = GSM3199737 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449124 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199738 !Sample_title = Cy72_CD45_F11_S743_comb !Sample_geo_accession = GSM3199738 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449123 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199739 !Sample_title = CY89COREQ1_A06_S294_comb !Sample_geo_accession = GSM3199739 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449122 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199740 !Sample_title = cy80_Cd45_pos_PD1_pos_C06_S66_comb !Sample_geo_accession = GSM3199740 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449121 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199741 !Sample_title = Cy72_CD45_C09_S705_comb !Sample_geo_accession = GSM3199741 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449120 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199742 !Sample_title = CY88CD45POS_7_D09_S237_comb !Sample_geo_accession = GSM3199742 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449119 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199743 !Sample_title = cy72_CD45_pos_D11_S911_comb !Sample_geo_accession = GSM3199743 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449118 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199744 !Sample_title = CY88CD45_150813_B07_S307_comb !Sample_geo_accession = GSM3199744 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449117 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199745 !Sample_title = cy53_1_CD45_pos_2_A12_S972_comb !Sample_geo_accession = GSM3199745 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449116 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199746 !Sample_title = cy60_1_cd_45_pos_3_H08_S380_comb !Sample_geo_accession = GSM3199746 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449115 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199747 !Sample_title = CY58_1_CD45_B07_S979_comb !Sample_geo_accession = GSM3199747 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449114 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199748 !Sample_title = cy79_p4_CD45_pos_PD1_pos_H11_S383_comb !Sample_geo_accession = GSM3199748 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449113 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199749 !Sample_title = cy80_Cd45_pos_PD1_pos_S112_E04_S112_comb !Sample_geo_accession = GSM3199749 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449112 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199750 !Sample_title = CY88CD45POS_2_C03_S411_comb !Sample_geo_accession = GSM3199750 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449111 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199751 !Sample_title = CY94CD45POS_1_A11_S107_comb !Sample_geo_accession = GSM3199751 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449110 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199752 !Sample_title = cy80_Cd45_pos_Pd1_neg_S368_H08_S368_comb !Sample_geo_accession = GSM3199752 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449109 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199753 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_B12_S216_comb !Sample_geo_accession = GSM3199753 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449108 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199754 !Sample_title = CY84_PRIM_POS_ALL_7_B12_S216_comb !Sample_geo_accession = GSM3199754 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449173 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199755 !Sample_title = cy94_cd45neg_cd90pos_E06_S342_comb !Sample_geo_accession = GSM3199755 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449107 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199756 !Sample_title = CY89A_Core_15_E12_S60_comb !Sample_geo_accession = GSM3199756 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449106 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199757 !Sample_title = cy53_1_CD45_pos_1_A07_S7_comb !Sample_geo_accession = GSM3199757 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449176 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199758 !Sample_title = cy88_cd_45_pos_G01_S457_comb !Sample_geo_accession = GSM3199758 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449175 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199759 !Sample_title = cy60_1_cd_45_pos_3_C01_S313_comb !Sample_geo_accession = GSM3199759 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449174 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199760 !Sample_title = Cy71_CD45_E04_S532_comb !Sample_geo_accession = GSM3199760 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449172 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199761 !Sample_title = cy94_cd45pos_H12_S192_comb !Sample_geo_accession = GSM3199761 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449171 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199762 !Sample_title = cy80_CD45_pos_PD1_pos_A05_S101_comb !Sample_geo_accession = GSM3199762 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449170 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199763 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_C09_S513_comb !Sample_geo_accession = GSM3199763 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449169 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199764 !Sample_title = cy74_CD45_pos_B01_S685_comb !Sample_geo_accession = GSM3199764 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449168 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199765 !Sample_title = cy84_Met_CD45_pos_B08_S884_comb !Sample_geo_accession = GSM3199765 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449167 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199766 !Sample_title = Cy72_CD45_F09_S741_comb !Sample_geo_accession = GSM3199766 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449166 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199767 !Sample_title = CY89A_CD45_POS_10_E07_S247_comb !Sample_geo_accession = GSM3199767 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449165 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199768 !Sample_title = cy94_cd45pos_H02_S182_comb !Sample_geo_accession = GSM3199768 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449164 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199769 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_G03_S459_comb !Sample_geo_accession = GSM3199769 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449163 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199770 !Sample_title = cy80_Cd45_pos_PD1_pos_S133_F01_S133_comb !Sample_geo_accession = GSM3199770 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449162 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199771 !Sample_title = Cy71_CD45_G04_S556_comb !Sample_geo_accession = GSM3199771 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449161 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199772 !Sample_title = cy74_CD45_pos_D01_S709_comb !Sample_geo_accession = GSM3199772 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449160 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199773 !Sample_title = cy80_CD45_pos_PD1_pos_E03_S147_comb !Sample_geo_accession = GSM3199773 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447954 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199774 !Sample_title = cy80_Cd45_pos_Pd1_neg_S362_H02_S362_comb !Sample_geo_accession = GSM3199774 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447953 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199775 !Sample_title = cy74_CD45_pos_G06_S750_comb !Sample_geo_accession = GSM3199775 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447952 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199776 !Sample_title = CY89FNA_C04_S220_comb !Sample_geo_accession = GSM3199776 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447951 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199777 !Sample_title = CY89A_CD45_POS_6_G04_S172_comb !Sample_geo_accession = GSM3199777 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447950 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199778 !Sample_title = CY89A_CD45_POS_6_A01_S97_comb !Sample_geo_accession = GSM3199778 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447949 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199779 !Sample_title = Cy81_FNA_CD45_G05_S269_comb !Sample_geo_accession = GSM3199779 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447948 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199780 !Sample_title = Cy81_FNA_CD45_H07_S283_comb !Sample_geo_accession = GSM3199780 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447947 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199781 !Sample_title = cy82_CD45_pos_3_B09_S21_comb !Sample_geo_accession = GSM3199781 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447946 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199782 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_D10_S430_comb !Sample_geo_accession = GSM3199782 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447944 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199783 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_F12_S264_comb !Sample_geo_accession = GSM3199783 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447943 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199784 !Sample_title = cy60_1_cd_45_pos_3_B04_S304_comb !Sample_geo_accession = GSM3199784 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447937 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199785 !Sample_title = CY89A_Core_15_H03_S87_comb !Sample_geo_accession = GSM3199785 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447942 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199786 !Sample_title = Cy71_CD45_G10_S562_comb !Sample_geo_accession = GSM3199786 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447941 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199787 !Sample_title = cy58_1_CD45_pos_F09_S645_comb !Sample_geo_accession = GSM3199787 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447940 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199788 !Sample_title = CY89A_Core_15_E03_S51_comb !Sample_geo_accession = GSM3199788 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447939 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199789 !Sample_title = cy74_CD45_pos_F06_S738_comb !Sample_geo_accession = GSM3199789 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447938 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199790 !Sample_title = cy80_CD45_pos_PD1_pos_D07_S139_comb !Sample_geo_accession = GSM3199790 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447936 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199791 !Sample_title = cy72_CD45_pos_G11_S947_comb !Sample_geo_accession = GSM3199791 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447935 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199792 !Sample_title = CY58_1_CD45_B05_S977_comb !Sample_geo_accession = GSM3199792 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447934 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199793 !Sample_title = cy79_p4_CD45_pos_PD1_pos_G01_S361_comb !Sample_geo_accession = GSM3199793 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447933 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199794 !Sample_title = cy80_CD45_neg_E06_S438_comb !Sample_geo_accession = GSM3199794 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447932 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199795 !Sample_title = cy60_1_cd_45_pos_4_C06_S30_comb !Sample_geo_accession = GSM3199795 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447931 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199796 !Sample_title = CY89COREQ1_A07_S295_comb !Sample_geo_accession = GSM3199796 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448001 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199797 !Sample_title = cy79_p5_CD45_pos_PD1_pos_F04_S640_comb !Sample_geo_accession = GSM3199797 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448000 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199798 !Sample_title = cy80_CD45_pos_PD1_pos_C08_S128_comb !Sample_geo_accession = GSM3199798 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447999 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199799 !Sample_title = cy79_p4_CD45_pos_PD1_pos_D10_S334_comb !Sample_geo_accession = GSM3199799 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447998 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199800 !Sample_title = Cy81_FNA_CD45_B07_S211_comb !Sample_geo_accession = GSM3199800 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447997 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199801 !Sample_title = CY58_1_CD45_C06_S990_comb !Sample_geo_accession = GSM3199801 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447996 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199802 !Sample_title = cy80_CD45_neg_D05_S425_comb !Sample_geo_accession = GSM3199802 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447995 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199803 !Sample_title = CY89A_Core_15_F08_S68_comb !Sample_geo_accession = GSM3199803 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449159 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199804 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_E02_S434_comb !Sample_geo_accession = GSM3199804 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449158 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199805 !Sample_title = cy84_Met_CD45_pos_F11_S935_comb !Sample_geo_accession = GSM3199805 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449157 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199806 !Sample_title = cy60_1_cd_45_pos_3_B08_S308_comb !Sample_geo_accession = GSM3199806 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449156 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199807 !Sample_title = Cy72_CD45_A02_S674_comb !Sample_geo_accession = GSM3199807 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449155 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199808 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_A02_S386_comb !Sample_geo_accession = GSM3199808 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449154 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199809 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_F06_S258_comb !Sample_geo_accession = GSM3199809 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449153 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199810 !Sample_title = cy53_1_CD45_pos_2_G11_S1043_comb !Sample_geo_accession = GSM3199810 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449152 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199811 !Sample_title = cy72_CD45_pos_G05_S941_comb !Sample_geo_accession = GSM3199811 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449151 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199812 !Sample_title = CY89A_CD45_POS_10_F11_S263_comb !Sample_geo_accession = GSM3199812 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449150 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199813 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_F01_S1117_comb !Sample_geo_accession = GSM3199813 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449149 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199814 !Sample_title = cy53_1_CD45_pos_1_E11_S59_comb !Sample_geo_accession = GSM3199814 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449208 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199815 !Sample_title = cy58_1_CD45_pos_B12_S600_comb !Sample_geo_accession = GSM3199815 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449148 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199816 !Sample_title = cy53_1_CD45_pos_1_F01_S61_comb !Sample_geo_accession = GSM3199816 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449147 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199817 !Sample_title = cy53_1_CD45_pos_2_G03_S1035_comb !Sample_geo_accession = GSM3199817 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449146 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199818 !Sample_title = cy80_CD45_neg_D08_S428_comb !Sample_geo_accession = GSM3199818 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449209 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199819 !Sample_title = Cy74_CD45_B04_S400_comb !Sample_geo_accession = GSM3199819 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449207 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199820 !Sample_title = cy94_cd45pos_B05_S113_comb !Sample_geo_accession = GSM3199820 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449206 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199821 !Sample_title = cy80_CD45_neg_E07_S439_comb !Sample_geo_accession = GSM3199821 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449205 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199822 !Sample_title = Cy72_CD45_H11_S767_comb !Sample_geo_accession = GSM3199822 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449204 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199823 !Sample_title = cy80_Cd45_pos_PD1_pos_S188_H08_S188_comb !Sample_geo_accession = GSM3199823 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449203 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199824 !Sample_title = cy60_1_cd_45_pos_4_B07_S19_comb !Sample_geo_accession = GSM3199824 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449202 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199825 !Sample_title = CY89A_CD45_POS_10_F12_S264_comb !Sample_geo_accession = GSM3199825 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449201 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199826 !Sample_title = cy79_p4_CD45_pos_PD1_pos_E12_S348_comb !Sample_geo_accession = GSM3199826 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449200 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199827 !Sample_title = cy80_CD45_pos_PD1_pos_A11_S107_comb !Sample_geo_accession = GSM3199827 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449199 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199828 !Sample_title = cy80_Cd45_pos_PD1_pos_A04_S16_comb !Sample_geo_accession = GSM3199828 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449198 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199829 !Sample_title = CY89FNA_G07_S271_comb !Sample_geo_accession = GSM3199829 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449197 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199830 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_H04_S280_comb !Sample_geo_accession = GSM3199830 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449196 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199831 !Sample_title = cy53_1_CD45_pos_2_A08_S968_comb !Sample_geo_accession = GSM3199831 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449195 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199832 !Sample_title = cy80_Cd45_pos_PD1_pos_S168_G12_S168_comb !Sample_geo_accession = GSM3199832 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449194 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199833 !Sample_title = Cy74_CD45_E11_S443_comb !Sample_geo_accession = GSM3199833 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448892 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199834 !Sample_title = Cy74_CD45_H04_S472_comb !Sample_geo_accession = GSM3199834 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448891 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199835 !Sample_title = cy60_1_cd_45_pos_4_G10_S82_comb !Sample_geo_accession = GSM3199835 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448890 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199836 !Sample_title = cy53_1_CD45_pos_2_C08_S992_comb !Sample_geo_accession = GSM3199836 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448889 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199837 !Sample_title = cy53_1_CD45_pos_2_H04_S1048_comb !Sample_geo_accession = GSM3199837 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448888 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199838 !Sample_title = CY88CD45_150813_H01_S373_comb !Sample_geo_accession = GSM3199838 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448887 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199839 !Sample_title = cy53_1_CD45_pos_2_E04_S1012_comb !Sample_geo_accession = GSM3199839 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448886 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199840 !Sample_title = CY75_1FNA_G06_S558_comb_8 !Sample_geo_accession = GSM3199840 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448885 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199841 !Sample_title = Cy72_CD45_E07_S727_comb !Sample_geo_accession = GSM3199841 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448884 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199842 !Sample_title = CY94CD45POS_1_H07_S187_comb !Sample_geo_accession = GSM3199842 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448883 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199843 !Sample_title = CY89A_CD45_POS_6_C05_S125_comb !Sample_geo_accession = GSM3199843 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448878 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199844 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_H10_S478_comb !Sample_geo_accession = GSM3199844 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448877 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199845 !Sample_title = CY89A_CD45_POS_10_A05_S197_comb !Sample_geo_accession = GSM3199845 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448882 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199846 !Sample_title = cy79_p4_CD45_pos_PD1_pos_D01_S325_comb !Sample_geo_accession = GSM3199846 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448881 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199847 !Sample_title = CY88CD45_150813_F11_S359_comb !Sample_geo_accession = GSM3199847 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448880 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199848 !Sample_title = cy79_p1_CD45_pos_PD1_pos_AS_C5_R1_B03_S207_comb !Sample_geo_accession = GSM3199848 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448879 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199849 !Sample_title = cy58_1_CD45_pos_H12_S672_comb !Sample_geo_accession = GSM3199849 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448876 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199850 !Sample_title = Cy81_FNA_CD45_H09_S285_comb !Sample_geo_accession = GSM3199850 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448875 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199851 !Sample_title = cy84_Primary_CD45_pos_H07_S475_comb !Sample_geo_accession = GSM3199851 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448873 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199852 !Sample_title = cy80_Cd45_pos_PD1_pos_D03_S87_comb !Sample_geo_accession = GSM3199852 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448872 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199853 !Sample_title = cy79_p4_CD45_pos_PD1_pos_H10_S382_comb !Sample_geo_accession = GSM3199853 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448871 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199854 !Sample_title = CY89COREQ1_B03_S303_comb !Sample_geo_accession = GSM3199854 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448870 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199855 !Sample_title = cy79_p4_CD45_pos_PD1_pos_B07_S307_comb !Sample_geo_accession = GSM3199855 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448869 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199856 !Sample_title = CY75_1FNA_B01_S493_comb !Sample_geo_accession = GSM3199856 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448868 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199857 !Sample_title = cy79_p4_CD45_pos_PD1_pos_C01_S313_comb !Sample_geo_accession = GSM3199857 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448867 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199858 !Sample_title = cy74_CD45_pos_B09_S693_comb !Sample_geo_accession = GSM3199858 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448866 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199859 !Sample_title = Cy74_CD45_D07_S427_comb !Sample_geo_accession = GSM3199859 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448865 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199860 !Sample_title = cy60_1_cd_45_pos_3_E10_S346_comb !Sample_geo_accession = GSM3199860 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448864 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199861 !Sample_title = CY89A_CD45_POS_6_H05_S185_comb !Sample_geo_accession = GSM3199861 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448863 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199862 !Sample_title = CY89A_CD45_POS_10_C11_S227_comb !Sample_geo_accession = GSM3199862 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448862 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199863 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_C11_S515_comb !Sample_geo_accession = GSM3199863 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448898 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199864 !Sample_title = CY89COREQ1_G02_S362_comb !Sample_geo_accession = GSM3199864 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448897 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199865 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_E09_S249_comb !Sample_geo_accession = GSM3199865 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448896 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199866 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_B08_S500_comb !Sample_geo_accession = GSM3199866 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448895 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199867 !Sample_title = CY89COREQ1_A01_S289_comb !Sample_geo_accession = GSM3199867 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448894 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199868 !Sample_title = Cy72_CD45_H02_S758_comb !Sample_geo_accession = GSM3199868 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448893 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199869 !Sample_title = CY88CD45POS_2_D12_S432_comb !Sample_geo_accession = GSM3199869 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448971 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199870 !Sample_title = cy94_cd45pos_4_E01_S49_comb !Sample_geo_accession = GSM3199870 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448970 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199871 !Sample_title = CY89NEG_C11_S35_comb !Sample_geo_accession = GSM3199871 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448969 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199872 !Sample_title = cy84_Primary_CD45_pos_E03_S435_comb !Sample_geo_accession = GSM3199872 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448968 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199873 !Sample_title = cy84_Met_CD45_pos_B03_S879_comb !Sample_geo_accession = GSM3199873 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448967 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199874 !Sample_title = CY84_PRIM_POS_ALL_7_C02_S218_comb !Sample_geo_accession = GSM3199874 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448966 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199875 !Sample_title = cy80_Cd45_pos_Pd1_neg_S366_H06_S366_comb !Sample_geo_accession = GSM3199875 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448965 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199876 !Sample_title = CY88CD45POS_2_G05_S461_comb !Sample_geo_accession = GSM3199876 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448964 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199877 !Sample_title = cy84_Met_CD45_pos_A09_S873_comb !Sample_geo_accession = GSM3199877 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448963 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199878 !Sample_title = CY88CD45_150813_C03_S315_comb !Sample_geo_accession = GSM3199878 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448962 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199879 !Sample_title = cy53_1_CD45_pos_2_H06_S1050_comb !Sample_geo_accession = GSM3199879 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448961 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199880 !Sample_title = cy94_cd45neg_cd90pos_E11_S347_comb !Sample_geo_accession = GSM3199880 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448960 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199881 !Sample_title = cy80_CD_90_pos_A04_S868_comb !Sample_geo_accession = GSM3199881 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448959 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199882 !Sample_title = cy80_CD_90_pos_F08_S932_comb !Sample_geo_accession = GSM3199882 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448958 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199883 !Sample_title = cy79_p3_CD45_pos_PD1_neg_A08_S104_comb !Sample_geo_accession = GSM3199883 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448957 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199884 !Sample_title = cy72_CD45_pos_H12_S960_comb !Sample_geo_accession = GSM3199884 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448956 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199885 !Sample_title = cy80_CD45_neg_G11_S947_comb !Sample_geo_accession = GSM3199885 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448955 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199886 !Sample_title = CY89A_Core_15_G04_S76_comb !Sample_geo_accession = GSM3199886 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448954 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199887 !Sample_title = CY89A_CD45_POS_10_E10_S250_comb !Sample_geo_accession = GSM3199887 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448953 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199888 !Sample_title = CY89A_CD45_POS_6_G07_S175_comb !Sample_geo_accession = GSM3199888 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448952 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199889 !Sample_title = cy88_cd45pos_5_F03_S255_comb !Sample_geo_accession = GSM3199889 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448951 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199890 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_F04_S736_comb !Sample_geo_accession = GSM3199890 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448950 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199891 !Sample_title = cy94_cd45neg_cd90pos_D08_S332_comb !Sample_geo_accession = GSM3199891 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448949 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199892 !Sample_title = CY94CD45POS_1_D02_S134_comb !Sample_geo_accession = GSM3199892 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448948 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199893 !Sample_title = cy80_Cd45_pos_Pd1_neg_S202_A10_S202_comb !Sample_geo_accession = GSM3199893 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448947 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199894 !Sample_title = Cy81_FNA_CD45_C12_S228_comb !Sample_geo_accession = GSM3199894 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448946 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199895 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_A10_S682_comb !Sample_geo_accession = GSM3199895 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448945 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199896 !Sample_title = CY89A_Core_15_B02_S14_comb !Sample_geo_accession = GSM3199896 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448944 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199897 !Sample_title = cy80_Cd45_pos_Pd1_neg_S201_A09_S201_comb !Sample_geo_accession = GSM3199897 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448943 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199898 !Sample_title = cy84_Primary_CD45_pos_H12_S480_comb !Sample_geo_accession = GSM3199898 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448942 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199899 !Sample_title = CY84_PRIM_POS_ALL_6_B03_S399_comb !Sample_geo_accession = GSM3199899 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448941 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199900 !Sample_title = cy84_Primary_CD45_pos_E08_S440_comb !Sample_geo_accession = GSM3199900 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448940 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199901 !Sample_title = cy88_cd45pos_5_F04_S256_comb !Sample_geo_accession = GSM3199901 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448939 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199902 !Sample_title = CY88CD45POS_2_F04_S448_comb !Sample_geo_accession = GSM3199902 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448938 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199903 !Sample_title = CY88CD45POS_2_D05_S425_comb !Sample_geo_accession = GSM3199903 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448937 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199904 !Sample_title = CY89COREQ1_H02_S374_comb !Sample_geo_accession = GSM3199904 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448936 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199905 !Sample_title = cy53_1_CD45_pos_2_A04_S964_comb !Sample_geo_accession = GSM3199905 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448935 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199906 !Sample_title = cy94_cd45neg_cd90pos_E02_S338_comb !Sample_geo_accession = GSM3199906 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448934 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199907 !Sample_title = cy94_cd45neg_cd90pos_H06_S378_comb !Sample_geo_accession = GSM3199907 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448933 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199908 !Sample_title = cy88_cd45pos_5_E03_S243_comb !Sample_geo_accession = GSM3199908 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448932 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199909 !Sample_title = CY58_1_CD45_F01_S1021_comb !Sample_geo_accession = GSM3199909 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448931 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199910 !Sample_title = cy80_Cd45_pos_Pd1_neg_S221_B05_S221_comb !Sample_geo_accession = GSM3199910 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448930 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199911 !Sample_title = cy79_p3_CD45_pos_PD1_neg_E01_S145_comb !Sample_geo_accession = GSM3199911 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448929 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199912 !Sample_title = cy94_cd45pos_G11_S179_comb !Sample_geo_accession = GSM3199912 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448928 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199913 !Sample_title = CY88_3_B02_S590_comb !Sample_geo_accession = GSM3199913 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449000 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199914 !Sample_title = CY88CD45POS_2_G03_S459_comb !Sample_geo_accession = GSM3199914 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448999 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199915 !Sample_title = cy84_Primary_CD45_pos_B03_S399_comb !Sample_geo_accession = GSM3199915 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448998 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199916 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_A02_S962_comb !Sample_geo_accession = GSM3199916 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448997 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199917 !Sample_title = cy79_p3_CD45_pos_PD1_neg_F07_S163_comb !Sample_geo_accession = GSM3199917 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448996 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199918 !Sample_title = cy88_cd_45_pos_G09_S465_comb !Sample_geo_accession = GSM3199918 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448995 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199919 !Sample_title = CY89FNAQ2_B12_S408_comb !Sample_geo_accession = GSM3199919 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448994 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199920 !Sample_title = CY89A_Core_15_D04_S40_comb !Sample_geo_accession = GSM3199920 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448993 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199921 !Sample_title = cy60_1_cd_45_pos_3_C12_S324_comb !Sample_geo_accession = GSM3199921 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448992 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199922 !Sample_title = cy94_cd45pos_C03_S123_comb !Sample_geo_accession = GSM3199922 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448991 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199923 !Sample_title = CY88CD45POS_2_D06_S426_comb !Sample_geo_accession = GSM3199923 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448990 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199924 !Sample_title = CY89A_CD45_POS_6_C10_S130_comb !Sample_geo_accession = GSM3199924 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448989 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199925 !Sample_title = cy80_Cd45_pos_PD1_pos_A03_S15_comb !Sample_geo_accession = GSM3199925 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448988 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199926 !Sample_title = cy94_cd45pos_D07_S139_comb !Sample_geo_accession = GSM3199926 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448987 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199927 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_D04_S808_comb !Sample_geo_accession = GSM3199927 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448986 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199928 !Sample_title = cy53_1_CD45_neg_D12_S336_comb !Sample_geo_accession = GSM3199928 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448985 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199929 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_C07_S991_comb !Sample_geo_accession = GSM3199929 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448984 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199930 !Sample_title = CY88CD45POS_2_E09_S441_comb !Sample_geo_accession = GSM3199930 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448983 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199931 !Sample_title = cy94_cd45pos_C07_S127_comb !Sample_geo_accession = GSM3199931 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448982 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199932 !Sample_title = cy53_1_CD45_pos_2_B03_S975_comb !Sample_geo_accession = GSM3199932 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448981 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199933 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_E05_S1013_comb !Sample_geo_accession = GSM3199933 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448980 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199934 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_B04_S496_comb !Sample_geo_accession = GSM3199934 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448979 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199935 !Sample_title = CY84_PRIM_POS_ALL_6_G06_S462_comb !Sample_geo_accession = GSM3199935 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448978 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199936 !Sample_title = Cy81_Bulk_CD45_B11_S119_comb !Sample_geo_accession = GSM3199936 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448977 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199937 !Sample_title = Cy72_CD45_E02_S722_comb !Sample_geo_accession = GSM3199937 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448976 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199938 !Sample_title = Cy72_CD45_D03_S711_comb !Sample_geo_accession = GSM3199938 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448975 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199939 !Sample_title = Cy81_Bulk_CD45_B07_S115_comb !Sample_geo_accession = GSM3199939 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448974 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199940 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_H05_S569_comb !Sample_geo_accession = GSM3199940 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448973 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199941 !Sample_title = cy72_CD45_pos_E01_S913_comb !Sample_geo_accession = GSM3199941 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448972 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199942 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_A09_S969_comb !Sample_geo_accession = GSM3199942 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449032 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199943 !Sample_title = CY89A_Core_15_B01_S13_comb !Sample_geo_accession = GSM3199943 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449031 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199944 !Sample_title = cy60_1_cd_45_pos_3_E01_S337_comb !Sample_geo_accession = GSM3199944 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449030 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199945 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_C11_S995_comb !Sample_geo_accession = GSM3199945 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449029 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199946 !Sample_title = CY89FNA_E08_S248_comb !Sample_geo_accession = GSM3199946 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449028 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199947 !Sample_title = cy88_cd45pos_5_C05_S221_comb !Sample_geo_accession = GSM3199947 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449027 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199948 !Sample_title = CY94CD45POS_1_F10_S166_comb !Sample_geo_accession = GSM3199948 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449026 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199949 !Sample_title = cy60_1_cd_45_pos_3_E12_S348_comb !Sample_geo_accession = GSM3199949 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449025 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199950 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_C01_S985_comb !Sample_geo_accession = GSM3199950 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449024 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199951 !Sample_title = cy88_cd_45_pos_3_F01_S637_comb !Sample_geo_accession = GSM3199951 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449023 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199952 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_F05_S545_comb !Sample_geo_accession = GSM3199952 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449022 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199953 !Sample_title = cy80_Cd45_pos_PD1_pos_D12_S96_comb !Sample_geo_accession = GSM3199953 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449021 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199954 !Sample_title = cy60_1_cd_45_pos_3_A04_S292_comb !Sample_geo_accession = GSM3199954 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449020 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199955 !Sample_title = cy82_CD45_neg_2_B02_S206_comb !Sample_geo_accession = GSM3199955 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449019 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199956 !Sample_title = cy80_CD45_neg_B11_S887_comb !Sample_geo_accession = GSM3199956 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449018 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199957 !Sample_title = cy80_Cd45_pos_Pd1_neg_S294_E06_S294_comb !Sample_geo_accession = GSM3199957 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449017 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199958 !Sample_title = cy72_CD45_pos_G03_S939_comb !Sample_geo_accession = GSM3199958 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449016 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199959 !Sample_title = cy72_CD45_pos_F08_S932_comb !Sample_geo_accession = GSM3199959 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449015 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199960 !Sample_title = CY94CD45POS_1_H09_S189_comb !Sample_geo_accession = GSM3199960 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449013 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199961 !Sample_title = cy53_1_CD45_neg_A03_S291_comb !Sample_geo_accession = GSM3199961 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449012 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199962 !Sample_title = CY89A_Core_15_A09_S9_comb !Sample_geo_accession = GSM3199962 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449011 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199963 !Sample_title = cy78_CD45_neg_2_C08_S608_comb !Sample_geo_accession = GSM3199963 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449010 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199964 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_F09_S1029_comb !Sample_geo_accession = GSM3199964 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449009 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199965 !Sample_title = CY88_5_F07_S739_comb !Sample_geo_accession = GSM3199965 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449014 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199966 !Sample_title = cy94_cd45neg_cd90pos_C10_S322_comb !Sample_geo_accession = GSM3199966 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449008 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199967 !Sample_title = cy94_cd45pos_C12_S132_comb !Sample_geo_accession = GSM3199967 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449007 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199968 !Sample_title = cy80_Cd45_pos_Pd1_neg_S228_B12_S228_comb !Sample_geo_accession = GSM3199968 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449006 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199969 !Sample_title = CY88CD45POS_2_A06_S390_comb !Sample_geo_accession = GSM3199969 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449005 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199970 !Sample_title = cy88_cd_45_pos_C01_S409_comb !Sample_geo_accession = GSM3199970 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449004 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199971 !Sample_title = cy53_1_CD45_neg_E02_S338_comb !Sample_geo_accession = GSM3199971 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449003 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199972 !Sample_title = cy94_cd45neg_cd90pos_G04_S364_comb !Sample_geo_accession = GSM3199972 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449002 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199973 !Sample_title = Cy80_II_CD45_G05_S941_comb !Sample_geo_accession = GSM3199973 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449001 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199974 !Sample_title = cy94_cd45pos_4_C10_S34_comb !Sample_geo_accession = GSM3199974 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449068 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199975 !Sample_title = CY84_PRIM_POS_ALL_7_D02_S230_comb !Sample_geo_accession = GSM3199975 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449067 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199976 !Sample_title = Cy72_CD45_H07_S763_comb !Sample_geo_accession = GSM3199976 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449066 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199977 !Sample_title = cy80_CD_90_pos_E04_S916_comb !Sample_geo_accession = GSM3199977 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449065 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199978 !Sample_title = cy80_Cd45_pos_Pd1_neg_S370_H10_S370_comb !Sample_geo_accession = GSM3199978 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449064 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199979 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_G08_S1136_comb !Sample_geo_accession = GSM3199979 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449063 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199980 !Sample_title = CY89A_Core_15_F03_S63_comb !Sample_geo_accession = GSM3199980 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449062 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199981 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_C06_S990_comb !Sample_geo_accession = GSM3199981 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449061 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199982 !Sample_title = CY88CD45POS_7_F09_S261_comb !Sample_geo_accession = GSM3199982 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449060 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199983 !Sample_title = CY88CD45_150813_D11_S335_comb !Sample_geo_accession = GSM3199983 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449455 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199984 !Sample_title = cy60_1_cd_45_pos_4_D01_S37_comb !Sample_geo_accession = GSM3199984 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449454 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199985 !Sample_title = cy94_cd45pos_F05_S161_comb !Sample_geo_accession = GSM3199985 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449453 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199986 !Sample_title = cy60_1_cd_45_pos_3_A03_S291_comb !Sample_geo_accession = GSM3199986 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449452 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199987 !Sample_title = cy82_CD45_neg_1_B09_S213_comb !Sample_geo_accession = GSM3199987 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449451 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199988 !Sample_title = Cy81_Bulk_CD45_D09_S141_comb !Sample_geo_accession = GSM3199988 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449450 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199989 !Sample_title = CY88CD45_150813_H07_S379_comb !Sample_geo_accession = GSM3199989 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449449 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199990 !Sample_title = cy80_CD45_neg_G07_S943_comb !Sample_geo_accession = GSM3199990 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449448 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199991 !Sample_title = cy78_CD45_neg_3_D02_S710_comb !Sample_geo_accession = GSM3199991 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449447 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199992 !Sample_title = cy60_1_cd_45_neg_3_H07_S571_comb !Sample_geo_accession = GSM3199992 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449446 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199993 !Sample_title = cy60_1_cd_45_pos_3_B01_S301_comb !Sample_geo_accession = GSM3199993 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449445 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199994 !Sample_title = cy72_CD45_pos_A11_S875_comb !Sample_geo_accession = GSM3199994 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449444 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199995 !Sample_title = CY89COREQ1_H03_S375_comb !Sample_geo_accession = GSM3199995 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449443 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199996 !Sample_title = cy80_CD45_pos_PD1_pos_B10_S118_comb !Sample_geo_accession = GSM3199996 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449442 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199997 !Sample_title = cy80_CD45_neg_D12_S912_comb !Sample_geo_accession = GSM3199997 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449441 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199998 !Sample_title = CY94CD45POS_1_D05_S137_comb !Sample_geo_accession = GSM3199998 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449440 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3199999 !Sample_title = cy60_1_cd_45_pos_3_A09_S297_comb !Sample_geo_accession = GSM3199999 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449439 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200000 !Sample_title = cy74_CD45_pos_D03_S711_comb !Sample_geo_accession = GSM3200000 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449438 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200001 !Sample_title = CY88CD45POS_7_C03_S219_comb !Sample_geo_accession = GSM3200001 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449437 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200002 !Sample_title = CY94CD45POS_1_F11_S167_comb !Sample_geo_accession = GSM3200002 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449436 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200003 !Sample_title = cy94_cd45neg_cd90pos_H04_S376_comb !Sample_geo_accession = GSM3200003 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449435 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200004 !Sample_title = cy79_p3_CD45_pos_PD1_neg_G01_S169_comb !Sample_geo_accession = GSM3200004 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449434 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200005 !Sample_title = CY94CD45POS_1_A12_S108_comb !Sample_geo_accession = GSM3200005 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449433 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200006 !Sample_title = CY84_PRIM_POS_ALL_7_H09_S285_comb !Sample_geo_accession = GSM3200006 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449432 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200007 !Sample_title = cy94_cd45neg_cd90pos_H12_S384_comb !Sample_geo_accession = GSM3200007 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449431 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200008 !Sample_title = cy53_1_CD45_pos_1_D01_S37_comb !Sample_geo_accession = GSM3200008 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449430 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200009 !Sample_title = cy82_CD45_pos_1_A04_S484_comb !Sample_geo_accession = GSM3200009 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449429 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200010 !Sample_title = cy60_1_cd_45_pos_4_E10_S58_comb !Sample_geo_accession = GSM3200010 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449497 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200011 !Sample_title = CY89A_CD45_POS_6_H04_S184_comb !Sample_geo_accession = GSM3200011 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449496 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200012 !Sample_title = CY84_PRIM_POS_ALL_7_E07_S247_comb !Sample_geo_accession = GSM3200012 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449495 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200013 !Sample_title = cy79_p3_CD45_pos_PD1_neg_C02_S122_comb !Sample_geo_accession = GSM3200013 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449291 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200014 !Sample_title = cy82_CD45_pos_1_G02_S554_comb !Sample_geo_accession = GSM3200014 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449290 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200015 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_H09_S1053_comb !Sample_geo_accession = GSM3200015 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449289 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200016 !Sample_title = cy94_cd45pos_4_B10_S22_comb !Sample_geo_accession = GSM3200016 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449288 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200017 !Sample_title = cy60_1_cd_45_pos_3_G01_S361_comb !Sample_geo_accession = GSM3200017 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449287 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200018 !Sample_title = cy53_1_CD45_pos_2_B08_S980_comb !Sample_geo_accession = GSM3200018 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449286 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200019 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_B12_S504_comb !Sample_geo_accession = GSM3200019 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449285 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200020 !Sample_title = cy80_CD_90_pos_E09_S921_comb !Sample_geo_accession = GSM3200020 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449284 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200021 !Sample_title = cy88_cd_45_pos_G07_S463_comb !Sample_geo_accession = GSM3200021 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449355 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200022 !Sample_title = cy80_CD45_pos_PD1_pos_H10_S190_comb !Sample_geo_accession = GSM3200022 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449354 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200023 !Sample_title = cy79_p3_CD45_pos_PD1_neg_B10_S118_comb !Sample_geo_accession = GSM3200023 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449353 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200024 !Sample_title = cy53_1_CD45_pos_1_B01_S13_comb !Sample_geo_accession = GSM3200024 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449352 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200025 !Sample_title = CY75_1FNA_A08_S488_comb !Sample_geo_accession = GSM3200025 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449283 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200026 !Sample_title = cy53_1_CD45_pos_1_A05_S5_comb !Sample_geo_accession = GSM3200026 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449282 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200027 !Sample_title = Cy74_CD45_E06_S438_comb !Sample_geo_accession = GSM3200027 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449356 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200028 !Sample_title = cy94_cd45neg_cd90pos_E05_S341_comb !Sample_geo_accession = GSM3200028 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449351 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200029 !Sample_title = cy94_cd45pos_4_E04_S52_comb !Sample_geo_accession = GSM3200029 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449350 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200030 !Sample_title = CY89FNAQ2_C11_S419_comb !Sample_geo_accession = GSM3200030 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449349 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200031 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_D04_S328_comb !Sample_geo_accession = GSM3200031 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449348 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200032 !Sample_title = Cy72_CD45_A07_S679_comb !Sample_geo_accession = GSM3200032 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449347 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200033 !Sample_title = CY88_5_F08_S740_comb !Sample_geo_accession = GSM3200033 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449346 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200034 !Sample_title = cy80_CD_90_pos_D10_S910_comb !Sample_geo_accession = GSM3200034 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449345 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200035 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_E06_S534_comb !Sample_geo_accession = GSM3200035 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449344 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200036 !Sample_title = CY89FNA_E05_S245_comb !Sample_geo_accession = GSM3200036 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449343 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200037 !Sample_title = CY89A_Core_15_F04_S64_comb !Sample_geo_accession = GSM3200037 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449342 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200038 !Sample_title = CY89COREQ1_G08_S368_comb !Sample_geo_accession = GSM3200038 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449341 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200039 !Sample_title = CY89COREQ1_E05_S341_comb !Sample_geo_accession = GSM3200039 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449340 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200040 !Sample_title = CY88CD45POS_2_D01_S421_comb !Sample_geo_accession = GSM3200040 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449339 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200041 !Sample_title = CY88CD45_150813_H12_S384_comb !Sample_geo_accession = GSM3200041 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449338 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200042 !Sample_title = Cy71_CD45_H05_S569_comb !Sample_geo_accession = GSM3200042 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449337 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200043 !Sample_title = cy84_Primary_CD45_pos_H08_S476_comb !Sample_geo_accession = GSM3200043 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449336 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200044 !Sample_title = cy84_Met_CD45_pos_H10_S958_comb !Sample_geo_accession = GSM3200044 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449335 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200045 !Sample_title = cy84_Primary_CD45_pos_B08_S404_comb !Sample_geo_accession = GSM3200045 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449334 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200046 !Sample_title = CY94CD45POS_1_D08_S140_comb !Sample_geo_accession = GSM3200046 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449333 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200047 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_G02_S554_comb !Sample_geo_accession = GSM3200047 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449332 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200048 !Sample_title = cy80_CD45_neg_C06_S894_comb !Sample_geo_accession = GSM3200048 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449331 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200049 !Sample_title = cy82_CD45_pos_2_D02_S518_comb !Sample_geo_accession = GSM3200049 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449330 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200050 !Sample_title = Cy71_CD45_H07_S571_comb !Sample_geo_accession = GSM3200050 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449329 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200051 !Sample_title = cy79_p3_CD45_pos_PD1_neg_E09_S153_comb !Sample_geo_accession = GSM3200051 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449328 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200052 !Sample_title = CY89A_CD45_POS_6_D06_S138_comb !Sample_geo_accession = GSM3200052 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449327 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200053 !Sample_title = cy53_1_CD45_neg_C01_S313_comb !Sample_geo_accession = GSM3200053 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449326 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200054 !Sample_title = cy53_1_CD45_neg_A10_S298_comb !Sample_geo_accession = GSM3200054 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449325 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200055 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_A07_S199_comb !Sample_geo_accession = GSM3200055 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449324 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200056 !Sample_title = CY89A_CD45_POS_6_F05_S161_comb !Sample_geo_accession = GSM3200056 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449323 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200057 !Sample_title = cy80_CD_90_pos_C11_S899_comb !Sample_geo_accession = GSM3200057 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449322 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200058 !Sample_title = CY88_3_H12_S672_comb !Sample_geo_accession = GSM3200058 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449393 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200059 !Sample_title = cy88_cd_45_pos_D09_S429_comb !Sample_geo_accession = GSM3200059 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449392 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200060 !Sample_title = cy79_p3_CD45_pos_PD1_neg_C06_S126_comb !Sample_geo_accession = GSM3200060 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449391 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200061 !Sample_title = cy80_CD_90_pos_C04_S892_comb !Sample_geo_accession = GSM3200061 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449390 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200062 !Sample_title = Cy72_CD45_E06_S726_comb !Sample_geo_accession = GSM3200062 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449389 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200063 !Sample_title = cy94_cd45neg_cd90pos_A10_S298_comb !Sample_geo_accession = GSM3200063 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449387 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200064 !Sample_title = Cy72_CD45_E11_S731_comb !Sample_geo_accession = GSM3200064 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449386 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200065 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_F04_S1024_comb !Sample_geo_accession = GSM3200065 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449385 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200066 !Sample_title = CY89A_Core_15_F05_S65_comb !Sample_geo_accession = GSM3200066 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449384 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200067 !Sample_title = cy74_CD45_pos_F02_S734_comb !Sample_geo_accession = GSM3200067 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449383 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200068 !Sample_title = CY94CD45POS_1_F08_S164_comb !Sample_geo_accession = GSM3200068 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449382 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200069 !Sample_title = Cy74_CD45_B08_S404_comb !Sample_geo_accession = GSM3200069 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449381 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200070 !Sample_title = cy60_1_cd_45_pos_3_B12_S312_comb !Sample_geo_accession = GSM3200070 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449380 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200071 !Sample_title = cy94_cd45neg_cd90pos_F04_S352_comb !Sample_geo_accession = GSM3200071 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449379 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200072 !Sample_title = cy94_cd45pos_C11_S131_comb !Sample_geo_accession = GSM3200072 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449378 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200073 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_E04_S1012_comb !Sample_geo_accession = GSM3200073 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449377 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200074 !Sample_title = cy80_Cd45_pos_Pd1_neg_S248_C08_S248_comb !Sample_geo_accession = GSM3200074 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449376 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200075 !Sample_title = cy84_Primary_CD45_pos_E06_S438_comb !Sample_geo_accession = GSM3200075 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449375 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200076 !Sample_title = CY88CD45POS_7_D01_S229_comb !Sample_geo_accession = GSM3200076 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449374 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200077 !Sample_title = cy80_CD_90_pos_B04_S880_comb !Sample_geo_accession = GSM3200077 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449373 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200078 !Sample_title = cy80_CD45_neg_E12_S924_comb !Sample_geo_accession = GSM3200078 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449372 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200079 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_B10_S982_comb !Sample_geo_accession = GSM3200079 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449371 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200080 !Sample_title = cy80_Cd45_pos_PD1_pos_A02_S14_comb !Sample_geo_accession = GSM3200080 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449370 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200081 !Sample_title = CY89A_CD45_POS_10_A11_S203_comb !Sample_geo_accession = GSM3200081 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449369 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200082 !Sample_title = cy60_1_cd_45_pos_4_G02_S74_comb !Sample_geo_accession = GSM3200082 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449368 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200083 !Sample_title = CY88_3_D06_S618_comb !Sample_geo_accession = GSM3200083 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449367 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200084 !Sample_title = cy94_cd45neg_cd90pos_B12_S312_comb !Sample_geo_accession = GSM3200084 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449366 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200085 !Sample_title = cy84_Primary_CD45_pos_G04_S460_comb !Sample_geo_accession = GSM3200085 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449365 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200086 !Sample_title = Cy72_CD45_G09_S753_comb !Sample_geo_accession = GSM3200086 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449364 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200087 !Sample_title = cy60_1_cd_45_pos_4_D12_S48_comb !Sample_geo_accession = GSM3200087 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449363 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200088 !Sample_title = cy80_CD45_neg_F12_S456_comb !Sample_geo_accession = GSM3200088 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449362 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200089 !Sample_title = CY94CD45POS_1_B05_S113_comb !Sample_geo_accession = GSM3200089 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449361 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200090 !Sample_title = cy80_CD45_neg_D02_S422_comb !Sample_geo_accession = GSM3200090 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449360 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200091 !Sample_title = CY94CD45POS_1_C04_S124_comb !Sample_geo_accession = GSM3200091 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449359 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200092 !Sample_title = CY88CD45POS_7_F05_S257_comb !Sample_geo_accession = GSM3200092 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449358 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200093 !Sample_title = cy84_Primary_CD45_neg_G03_S1035_comb !Sample_geo_accession = GSM3200093 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449357 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200094 !Sample_title = cy94_cd45neg_cd90pos_E12_S348_comb !Sample_geo_accession = GSM3200094 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449428 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200095 !Sample_title = cy82_CD45_pos_1_B12_S504_comb !Sample_geo_accession = GSM3200095 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449427 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200096 !Sample_title = cy94_cd45pos_C08_S128_comb !Sample_geo_accession = GSM3200096 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449426 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200097 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_B04_S784_comb !Sample_geo_accession = GSM3200097 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449425 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200098 !Sample_title = cy80_Cd45_pos_Pd1_neg_S222_B06_S222_comb !Sample_geo_accession = GSM3200098 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449424 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200099 !Sample_title = CY88CD45POS_7_D03_S231_comb !Sample_geo_accession = GSM3200099 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449423 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200100 !Sample_title = cy88_cd_45_pos_E05_S437_comb !Sample_geo_accession = GSM3200100 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449422 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200101 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_D06_S1002_comb !Sample_geo_accession = GSM3200101 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449421 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200102 !Sample_title = cy88_cd_45_pos_G05_S461_comb !Sample_geo_accession = GSM3200102 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449420 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200103 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_F09_S549_comb !Sample_geo_accession = GSM3200103 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449419 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200104 !Sample_title = cy53_1_CD45_pos_2_B12_S984_comb !Sample_geo_accession = GSM3200104 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449418 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200105 !Sample_title = CY94CD45POS_1_F05_S161_comb !Sample_geo_accession = GSM3200105 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449417 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200106 !Sample_title = cy58_1_CD45_pos_H05_S665_comb !Sample_geo_accession = GSM3200106 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449416 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200107 !Sample_title = cy94_cd45pos_4_D06_S42_comb !Sample_geo_accession = GSM3200107 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449415 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200108 !Sample_title = Cy74_CD45_H08_S476_comb !Sample_geo_accession = GSM3200108 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449414 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200109 !Sample_title = CY94CD45POS_1_B02_S110_comb !Sample_geo_accession = GSM3200109 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449413 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200110 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_A05_S965_comb !Sample_geo_accession = GSM3200110 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449412 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200111 !Sample_title = cy94_cd45pos_4_G08_S80_comb !Sample_geo_accession = GSM3200111 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449411 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200112 !Sample_title = cy84_Primary_CD45_pos_E01_S433_comb !Sample_geo_accession = GSM3200112 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449410 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200113 !Sample_title = cy80_Cd45_pos_Pd1_neg_S204_A12_S204_comb !Sample_geo_accession = GSM3200113 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449409 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200114 !Sample_title = cy58_1_CD45_pos_B10_S598_comb !Sample_geo_accession = GSM3200114 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449403 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200115 !Sample_title = CY89A_Core_15_E05_S53_comb !Sample_geo_accession = GSM3200115 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449408 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200116 !Sample_title = cy94_cd45pos_D04_S136_comb !Sample_geo_accession = GSM3200116 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449407 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200117 !Sample_title = CY94CD45POS_1_B06_S114_comb !Sample_geo_accession = GSM3200117 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449406 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200118 !Sample_title = Cy74_CD45_C03_S411_comb !Sample_geo_accession = GSM3200118 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449405 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200119 !Sample_title = Cy71_CD45_A04_S484_comb !Sample_geo_accession = GSM3200119 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449404 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200120 !Sample_title = cy80_Cd45_pos_Pd1_neg_S197_A05_S197_comb !Sample_geo_accession = GSM3200120 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449402 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200121 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_E07_S535_comb !Sample_geo_accession = GSM3200121 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449401 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200122 !Sample_title = cy74_CD45_pos_G12_S756_comb !Sample_geo_accession = GSM3200122 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449400 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200123 !Sample_title = CY88CD45_150813_F03_S351_comb !Sample_geo_accession = GSM3200123 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449399 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200124 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_C12_S996_comb !Sample_geo_accession = GSM3200124 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449397 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200125 !Sample_title = CY89A_CD45_POS_6_G06_S174_comb !Sample_geo_accession = GSM3200125 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449396 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200126 !Sample_title = cy80_CD45_neg_C08_S416_comb !Sample_geo_accession = GSM3200126 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449395 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200127 !Sample_title = cy94_cd45neg_cd90pos_H11_S383_comb !Sample_geo_accession = GSM3200127 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449394 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200128 !Sample_title = CY89A_Core_15_C12_S36_comb !Sample_geo_accession = GSM3200128 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449460 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200129 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_H04_S568_comb !Sample_geo_accession = GSM3200129 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449459 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200130 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_B11_S311_comb !Sample_geo_accession = GSM3200130 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449458 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200131 !Sample_title = cy60_1_cd_45_pos_4_E06_S54_comb !Sample_geo_accession = GSM3200131 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449457 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200132 !Sample_title = cy60_1_cd_45_pos_4_A10_S10_comb !Sample_geo_accession = GSM3200132 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449456 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200133 !Sample_title = CY94CD45POS_1_H05_S185_comb !Sample_geo_accession = GSM3200133 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448642 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200134 !Sample_title = cy79_p5_CD45_pos_PD1_pos_D09_S621_comb !Sample_geo_accession = GSM3200134 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448641 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200135 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_B03_S495_comb !Sample_geo_accession = GSM3200135 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448640 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200136 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_E04_S532_comb !Sample_geo_accession = GSM3200136 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448639 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200137 !Sample_title = CY94CD45POS_1_E02_S146_comb !Sample_geo_accession = GSM3200137 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448638 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200138 !Sample_title = cy80_Cd45_pos_Pd1_neg_S203_A11_S203_comb !Sample_geo_accession = GSM3200138 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448637 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200139 !Sample_title = cy94_cd45pos_4_D07_S43_comb !Sample_geo_accession = GSM3200139 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448636 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200140 !Sample_title = cy94_cd45pos_C04_S124_comb !Sample_geo_accession = GSM3200140 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448635 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200141 !Sample_title = Cy81_Bulk_CD45_D05_S137_comb !Sample_geo_accession = GSM3200141 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448634 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200142 !Sample_title = cy53_1_CD45_neg_C04_S316_comb !Sample_geo_accession = GSM3200142 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448633 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200143 !Sample_title = CY94CD45POS_1_C01_S121_comb !Sample_geo_accession = GSM3200143 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448632 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200144 !Sample_title = cy79_p3_CD45_pos_PD1_neg_C11_S131_comb !Sample_geo_accession = GSM3200144 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448631 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200145 !Sample_title = cy74_CD45_pos_D12_S720_comb !Sample_geo_accession = GSM3200145 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448630 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200146 !Sample_title = cy80_Cd45_pos_Pd1_neg_S276_D12_S276_comb !Sample_geo_accession = GSM3200146 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448629 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200147 !Sample_title = cy80_CD45_pos_PD1_pos_E09_S153_comb !Sample_geo_accession = GSM3200147 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448628 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200148 !Sample_title = Cy72_CD45_A05_S677_comb !Sample_geo_accession = GSM3200148 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448627 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200149 !Sample_title = cy80_CD45_neg_D07_S427_comb !Sample_geo_accession = GSM3200149 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448626 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200150 !Sample_title = CY88_5_F12_S744_comb !Sample_geo_accession = GSM3200150 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448625 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200151 !Sample_title = cy60_1_cd_45_pos_3_G02_S362_comb !Sample_geo_accession = GSM3200151 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448624 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200152 !Sample_title = CY89A_CD45_POS_6_F06_S162_comb !Sample_geo_accession = GSM3200152 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448623 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200153 !Sample_title = cy94_cd45pos_4_E10_S58_comb !Sample_geo_accession = GSM3200153 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448622 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200154 !Sample_title = cy80_CD45_neg_B05_S401_comb !Sample_geo_accession = GSM3200154 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448621 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200155 !Sample_title = CY89A_CD45_POS_10_B11_S215_comb !Sample_geo_accession = GSM3200155 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448620 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200156 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_G01_S1129_comb !Sample_geo_accession = GSM3200156 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448619 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200157 !Sample_title = CY88CD45POS_2_F06_S450_comb !Sample_geo_accession = GSM3200157 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448618 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200158 !Sample_title = cy94_cd45pos_H04_S184_comb !Sample_geo_accession = GSM3200158 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448617 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200159 !Sample_title = CY89A_Core_15_H02_S86_comb !Sample_geo_accession = GSM3200159 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448616 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200160 !Sample_title = cy80_Cd45_pos_Pd1_neg_S275_D11_S275_comb !Sample_geo_accession = GSM3200160 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448615 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200161 !Sample_title = cy94_cd45neg_cd90pos_G05_S365_comb !Sample_geo_accession = GSM3200161 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448614 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200162 !Sample_title = Cy71_CD45_D04_S520_comb !Sample_geo_accession = GSM3200162 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448613 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200163 !Sample_title = cy94_cd45neg_cd90pos_B04_S304_comb !Sample_geo_accession = GSM3200163 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447868 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200164 !Sample_title = cy60_1_cd_45_pos_4_E07_S55_comb !Sample_geo_accession = GSM3200164 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447867 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200165 !Sample_title = cy53_1_CD45_pos_1_F04_S64_comb !Sample_geo_accession = GSM3200165 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447866 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200166 !Sample_title = CY89A_Core_15_A07_S7_comb !Sample_geo_accession = GSM3200166 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447865 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200167 !Sample_title = cy80_CD45_neg_F12_S936_comb !Sample_geo_accession = GSM3200167 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447864 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200168 !Sample_title = cy80_CD45_neg_F10_S934_comb !Sample_geo_accession = GSM3200168 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447863 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200169 !Sample_title = CY89FNA_D05_S233_comb !Sample_geo_accession = GSM3200169 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447862 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200170 !Sample_title = CY94CD45POS_1_A06_S102_comb !Sample_geo_accession = GSM3200170 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447860 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200171 !Sample_title = cy80_Cd45_pos_Pd1_neg_S241_C01_S241_comb !Sample_geo_accession = GSM3200171 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447859 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200172 !Sample_title = cy82_CD45_pos_2_G12_S564_comb !Sample_geo_accession = GSM3200172 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447930 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200173 !Sample_title = CY84_PRIM_POS_ALL_8_E07_S151_comb !Sample_geo_accession = GSM3200173 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447929 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200174 !Sample_title = CY89A_Core_15_B03_S15_comb !Sample_geo_accession = GSM3200174 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447928 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200175 !Sample_title = cy80_CD45_neg_A10_S394_comb !Sample_geo_accession = GSM3200175 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447861 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200176 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_G06_S558_comb !Sample_geo_accession = GSM3200176 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447927 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200177 !Sample_title = cy80_CD_90_pos_F09_S933_comb !Sample_geo_accession = GSM3200177 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447926 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200178 !Sample_title = CY94CD45POS_1_D07_S139_comb !Sample_geo_accession = GSM3200178 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447925 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200179 !Sample_title = cy94_cd45pos_B06_S114_comb !Sample_geo_accession = GSM3200179 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447924 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200180 !Sample_title = CY84_PRIM_POS_ALL_7_B06_S210_comb !Sample_geo_accession = GSM3200180 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447923 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200181 !Sample_title = cy80_CD_90_pos_F03_S927_comb !Sample_geo_accession = GSM3200181 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447922 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200182 !Sample_title = CY58_1_CD45_B06_S978_comb !Sample_geo_accession = GSM3200182 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447921 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200183 !Sample_title = cy88_cd_45_pos_E06_S438_comb !Sample_geo_accession = GSM3200183 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447920 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200184 !Sample_title = cy84_Primary_CD45_pos_G02_S458_comb !Sample_geo_accession = GSM3200184 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447919 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200185 !Sample_title = cy79_p4_CD45_pos_PD1_pos_B10_S310_comb !Sample_geo_accession = GSM3200185 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447918 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200186 !Sample_title = CY84_PRIM_POS_ALL_8_B11_S119_comb !Sample_geo_accession = GSM3200186 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447917 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200187 !Sample_title = cy94_cd45pos_4_B06_S18_comb !Sample_geo_accession = GSM3200187 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447916 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200188 !Sample_title = cy94_cd45pos_4_D08_S44_comb !Sample_geo_accession = GSM3200188 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447915 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200189 !Sample_title = cy60_1_cd_45_pos_3_F03_S351_comb !Sample_geo_accession = GSM3200189 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447914 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200190 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_D02_S998_comb !Sample_geo_accession = GSM3200190 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447913 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200191 !Sample_title = CY89FNA_A03_S195_comb !Sample_geo_accession = GSM3200191 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447912 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200192 !Sample_title = cy74_CD45_pos_E07_S727_comb !Sample_geo_accession = GSM3200192 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447911 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200193 !Sample_title = CY88CD45_150813_F06_S354_comb !Sample_geo_accession = GSM3200193 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448201 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200194 !Sample_title = cy88_cd_45_pos_B11_S407_comb !Sample_geo_accession = GSM3200194 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448200 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200195 !Sample_title = Cy74_CD45_D08_S428_comb !Sample_geo_accession = GSM3200195 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448199 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200196 !Sample_title = cy53_1_CD45_neg_H05_S377_comb !Sample_geo_accession = GSM3200196 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448198 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200197 !Sample_title = Cy72_CD45_A01_S673_comb !Sample_geo_accession = GSM3200197 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448197 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200198 !Sample_title = CY89A_Core_15_G12_S84_comb !Sample_geo_accession = GSM3200198 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448196 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200199 !Sample_title = cy53_1_CD45_pos_2_D05_S1001_comb !Sample_geo_accession = GSM3200199 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448195 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200200 !Sample_title = CY89A_CD45_POS_6_E01_S145_comb !Sample_geo_accession = GSM3200200 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448194 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200201 !Sample_title = cy80_Cd45_pos_Pd1_neg_S339_G03_S339_comb !Sample_geo_accession = GSM3200201 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448259 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200202 !Sample_title = cy72_CD45_pos_B06_S882_comb !Sample_geo_accession = GSM3200202 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448258 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200203 !Sample_title = cy79_p3_CD45_pos_PD1_neg_E11_S155_comb !Sample_geo_accession = GSM3200203 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448257 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200204 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_C12_S516_comb !Sample_geo_accession = GSM3200204 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448256 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200205 !Sample_title = cy94_cd45pos_A09_S105_comb !Sample_geo_accession = GSM3200205 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448255 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200206 !Sample_title = Cy74_CD45_C10_S418_comb !Sample_geo_accession = GSM3200206 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448254 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200207 !Sample_title = CY89FNA_C06_S222_comb !Sample_geo_accession = GSM3200207 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448253 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200208 !Sample_title = CY84_PRIM_POS_ALL_8_E10_S154_comb !Sample_geo_accession = GSM3200208 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448252 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200209 !Sample_title = cy60_1_cd_45_pos_3_H10_S382_comb !Sample_geo_accession = GSM3200209 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448251 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200210 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_H06_S1050_comb !Sample_geo_accession = GSM3200210 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448250 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200211 !Sample_title = cy94_cd45pos_F01_S157_comb !Sample_geo_accession = GSM3200211 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448249 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200212 !Sample_title = cy53_1_CD45_pos_1_D06_S42_comb !Sample_geo_accession = GSM3200212 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448248 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200213 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_B01_S973_comb !Sample_geo_accession = GSM3200213 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448247 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200214 !Sample_title = cy53_1_CD45_neg_H03_S375_comb !Sample_geo_accession = GSM3200214 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448246 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200215 !Sample_title = CY88CD45POS_2_F02_S446_comb !Sample_geo_accession = GSM3200215 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448245 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200216 !Sample_title = cy80_CD45_neg_B05_S881_comb !Sample_geo_accession = GSM3200216 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448244 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200217 !Sample_title = cy60_1_cd_45_pos_4_A12_S12_comb !Sample_geo_accession = GSM3200217 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448243 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200218 !Sample_title = Cy81_Bulk_CD45_F04_S160_comb !Sample_geo_accession = GSM3200218 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448242 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200219 !Sample_title = cy60_1_cd_45_pos_3_F05_S353_comb !Sample_geo_accession = GSM3200219 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448241 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200220 !Sample_title = CY89FNAQ2_D04_S424_comb !Sample_geo_accession = GSM3200220 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448240 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200221 !Sample_title = cy58_1_CD45_pos_H11_S671_comb !Sample_geo_accession = GSM3200221 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448239 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200222 !Sample_title = CY89COREQ1_E11_S347_comb !Sample_geo_accession = GSM3200222 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448238 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200223 !Sample_title = cy84_Primary_CD45_pos_A04_S388_comb !Sample_geo_accession = GSM3200223 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448237 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200224 !Sample_title = cy53_1_CD45_neg_G10_S370_comb !Sample_geo_accession = GSM3200224 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448236 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200225 !Sample_title = cy60_1_cd_45_pos_3_D05_S329_comb !Sample_geo_accession = GSM3200225 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448235 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200226 !Sample_title = CY89COREQ1_C02_S314_comb !Sample_geo_accession = GSM3200226 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448234 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200227 !Sample_title = cy80_Cd45_pos_PD1_pos_C12_S72_comb !Sample_geo_accession = GSM3200227 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448233 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200228 !Sample_title = cy94_cd45pos_4_C06_S30_comb !Sample_geo_accession = GSM3200228 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448232 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200229 !Sample_title = cy80_CD45_neg_H05_S953_comb !Sample_geo_accession = GSM3200229 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448231 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200230 !Sample_title = cy60_1_cd_45_pos_3_E08_S344_comb !Sample_geo_accession = GSM3200230 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448230 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200231 !Sample_title = cy94_cd45neg_cd90pos_F01_S349_comb !Sample_geo_accession = GSM3200231 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448229 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200232 !Sample_title = Cy72_CD45_G03_S747_comb !Sample_geo_accession = GSM3200232 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448294 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200233 !Sample_title = CY89A_CD45_POS_6_B07_S115_comb !Sample_geo_accession = GSM3200233 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448293 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200234 !Sample_title = cy84_Primary_CD45_pos_A12_S396_comb !Sample_geo_accession = GSM3200234 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448292 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200235 !Sample_title = CY94CD45POS_1_G02_S170_comb !Sample_geo_accession = GSM3200235 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448291 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200236 !Sample_title = cy78_CD45_neg_3_B03_S687_comb !Sample_geo_accession = GSM3200236 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448290 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200237 !Sample_title = CY89A_CD45_POS_6_C11_S131_comb !Sample_geo_accession = GSM3200237 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448288 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200238 !Sample_title = cy80_CD45_pos_PD1_pos_E01_S145_comb !Sample_geo_accession = GSM3200238 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448287 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200239 !Sample_title = CY89FNA_G05_S269_comb !Sample_geo_accession = GSM3200239 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448286 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200240 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_A12_S972_comb !Sample_geo_accession = GSM3200240 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448285 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200241 !Sample_title = cy82_CD45_pos_2_D09_S525_comb !Sample_geo_accession = GSM3200241 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448284 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200242 !Sample_title = CY89FNA_E07_S247_comb !Sample_geo_accession = GSM3200242 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448283 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200243 !Sample_title = CY89FNA_B07_S211_comb !Sample_geo_accession = GSM3200243 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448282 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200244 !Sample_title = cy80_CD45_neg_G02_S458_comb !Sample_geo_accession = GSM3200244 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448281 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200245 !Sample_title = cy53_1_CD45_pos_1_H12_S96_comb !Sample_geo_accession = GSM3200245 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448280 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200246 !Sample_title = CY89FNA_G03_S267_comb !Sample_geo_accession = GSM3200246 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448279 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200247 !Sample_title = cy94_cd45pos_H09_S189_comb !Sample_geo_accession = GSM3200247 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448278 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200248 !Sample_title = cy82_CD45_pos_1_C12_S516_comb !Sample_geo_accession = GSM3200248 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448277 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200249 !Sample_title = CY88CD45_150813_C02_S314_comb !Sample_geo_accession = GSM3200249 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448276 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200250 !Sample_title = cy60_1_cd_45_pos_3_C10_S322_comb !Sample_geo_accession = GSM3200250 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448275 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200251 !Sample_title = cy79_p3_CD45_pos_PD1_neg_F03_S159_comb !Sample_geo_accession = GSM3200251 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448274 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200252 !Sample_title = cy82_CD45_pos_3_C08_S32_comb !Sample_geo_accession = GSM3200252 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448273 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200253 !Sample_title = cy80_Cd45_pos_PD1_pos_S136_F04_S136_comb !Sample_geo_accession = GSM3200253 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448097 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200254 !Sample_title = Cy81_Bulk_CD45_C09_S129_comb !Sample_geo_accession = GSM3200254 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448096 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200255 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_G03_S555_comb !Sample_geo_accession = GSM3200255 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448095 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200256 !Sample_title = cy53_1_CD45_pos_2_B09_S981_comb !Sample_geo_accession = GSM3200256 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448094 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200257 !Sample_title = cy80_Cd45_pos_Pd1_neg_S245_C05_S245_comb !Sample_geo_accession = GSM3200257 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448093 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200258 !Sample_title = Cy72_CD45_B09_S693_comb !Sample_geo_accession = GSM3200258 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448092 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200259 !Sample_title = cy94_cd45pos_4_C02_S26_comb !Sample_geo_accession = GSM3200259 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448091 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200260 !Sample_title = cy94_cd45neg_cd90pos_A01_S289_comb !Sample_geo_accession = GSM3200260 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448090 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200261 !Sample_title = cy80_CD45_neg_F10_S454_comb !Sample_geo_accession = GSM3200261 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448089 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200262 !Sample_title = cy84_Primary_CD45_pos_D12_S432_comb !Sample_geo_accession = GSM3200262 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448088 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200263 !Sample_title = cy80_Cd45_pos_Pd1_neg_S297_E09_S297_comb !Sample_geo_accession = GSM3200263 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448087 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200264 !Sample_title = CY89A_CD45_POS_6_A11_S107_comb !Sample_geo_accession = GSM3200264 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448081 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200265 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_D08_S524_comb !Sample_geo_accession = GSM3200265 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448086 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200266 !Sample_title = CY88CD45_150813_H06_S378_comb !Sample_geo_accession = GSM3200266 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448085 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200267 !Sample_title = CY89COREQ1_F05_S353_comb !Sample_geo_accession = GSM3200267 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448084 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200268 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_A10_S970_comb !Sample_geo_accession = GSM3200268 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448083 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200269 !Sample_title = cy60_1_cd_45_pos_3_E05_S341_comb !Sample_geo_accession = GSM3200269 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448082 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200270 !Sample_title = cy72_CD45_pos_H04_S952_comb !Sample_geo_accession = GSM3200270 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448080 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200271 !Sample_title = cy80_CD_90_pos_F07_S931_comb !Sample_geo_accession = GSM3200271 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448079 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200272 !Sample_title = cy60_1_cd_45_pos_3_D03_S327_comb !Sample_geo_accession = GSM3200272 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448078 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200273 !Sample_title = Cy81_Bulk_CD45_G05_S173_comb !Sample_geo_accession = GSM3200273 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448077 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200274 !Sample_title = cy94_cd45pos_B04_S112_comb !Sample_geo_accession = GSM3200274 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448076 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200275 !Sample_title = cy80_CD45_pos_PD1_pos_H01_S181_comb !Sample_geo_accession = GSM3200275 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448075 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200276 !Sample_title = CY88CD45POS_2_H11_S479_comb !Sample_geo_accession = GSM3200276 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448074 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200277 !Sample_title = CY89A_Core_15_C04_S28_comb !Sample_geo_accession = GSM3200277 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448073 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200278 !Sample_title = CY84_PRIM_POS_ALL_8_B06_S114_comb !Sample_geo_accession = GSM3200278 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448072 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200279 !Sample_title = cy80_Cd45_pos_Pd1_neg_S291_E03_S291_comb !Sample_geo_accession = GSM3200279 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448071 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200280 !Sample_title = cy80_Cd45_pos_Pd1_neg_S266_D02_S266_comb !Sample_geo_accession = GSM3200280 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448070 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200281 !Sample_title = cy53_1_CD45_pos_1_A09_S9_comb !Sample_geo_accession = GSM3200281 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448069 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200282 !Sample_title = CY89FNA_F08_S260_comb !Sample_geo_accession = GSM3200282 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448068 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200283 !Sample_title = CY89A_CD45_POS_10_A02_S194_comb !Sample_geo_accession = GSM3200283 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448167 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200284 !Sample_title = Cy81_Bulk_CD45_G08_S176_comb !Sample_geo_accession = GSM3200284 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448166 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200285 !Sample_title = cy80_CD45_neg_E09_S441_comb !Sample_geo_accession = GSM3200285 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448165 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200286 !Sample_title = cy80_CD_90_pos_C05_S893_comb !Sample_geo_accession = GSM3200286 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448228 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200287 !Sample_title = cy60_1_cd_45_pos_4_E08_S56_comb !Sample_geo_accession = GSM3200287 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448227 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200288 !Sample_title = CY94CD45POS_1_C03_S123_comb !Sample_geo_accession = GSM3200288 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448226 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200289 !Sample_title = cy80_Cd45_pos_Pd1_neg_S342_G06_S342_comb !Sample_geo_accession = GSM3200289 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448225 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200290 !Sample_title = CY88CD45_150813_D09_S333_comb !Sample_geo_accession = GSM3200290 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448224 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200291 !Sample_title = CY89A_CD45_POS_6_F01_S157_comb !Sample_geo_accession = GSM3200291 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448223 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200292 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_A12_S12_comb !Sample_geo_accession = GSM3200292 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448222 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200293 !Sample_title = cy80_Cd45_pos_Pd1_neg_S369_H09_S369_comb !Sample_geo_accession = GSM3200293 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448221 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200294 !Sample_title = CY84_PRIM_POS_ALL_7_F12_S264_comb !Sample_geo_accession = GSM3200294 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448220 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200295 !Sample_title = CY84_PRIM_POS_ALL_7_F02_S254_comb !Sample_geo_accession = GSM3200295 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448219 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200296 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_B05_S977_comb !Sample_geo_accession = GSM3200296 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448218 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200297 !Sample_title = cy94_cd45neg_cd90pos_F11_S359_comb !Sample_geo_accession = GSM3200297 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448217 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200298 !Sample_title = cy79_p3_CD45_pos_PD1_neg_F01_S157_comb !Sample_geo_accession = GSM3200298 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448216 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200299 !Sample_title = cy80_CD45_neg_D01_S421_comb !Sample_geo_accession = GSM3200299 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448215 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200300 !Sample_title = CY89A_Core_15_G02_S74_comb !Sample_geo_accession = GSM3200300 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448214 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200301 !Sample_title = Cy72_CD45_C10_S706_comb !Sample_geo_accession = GSM3200301 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448213 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200302 !Sample_title = CY88CD45_150813_C11_S323_comb !Sample_geo_accession = GSM3200302 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448212 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200303 !Sample_title = cy53_1_CD45_neg_B11_S311_comb !Sample_geo_accession = GSM3200303 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448211 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200304 !Sample_title = cy79_p3_CD45_pos_PD1_neg_B12_S120_comb !Sample_geo_accession = GSM3200304 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448210 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200305 !Sample_title = CY94CD45POS_1_G10_S178_comb !Sample_geo_accession = GSM3200305 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448209 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200306 !Sample_title = cy80_CD45_pos_PD1_pos_B08_S116_comb !Sample_geo_accession = GSM3200306 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448208 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200307 !Sample_title = CY94CD45POS_1_A05_S101_comb !Sample_geo_accession = GSM3200307 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448207 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200308 !Sample_title = cy53_1_CD45_neg_D07_S331_comb !Sample_geo_accession = GSM3200308 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448206 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200309 !Sample_title = cy88_cd_45_pos_F06_S450_comb !Sample_geo_accession = GSM3200309 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448205 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200310 !Sample_title = cy82_CD45_pos_2_H04_S568_comb !Sample_geo_accession = GSM3200310 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448204 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200311 !Sample_title = cy88_cd45pos_5_E04_S244_comb !Sample_geo_accession = GSM3200311 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448203 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200312 !Sample_title = CY89COREQ1_E06_S342_comb !Sample_geo_accession = GSM3200312 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448202 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200313 !Sample_title = cy88_cd45pos_5_D01_S229_comb !Sample_geo_accession = GSM3200313 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448272 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200314 !Sample_title = cy88_cd45pos_5_D02_S230_comb !Sample_geo_accession = GSM3200314 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448271 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200315 !Sample_title = cy79_p3_CD45_pos_PD1_neg_C07_S127_comb !Sample_geo_accession = GSM3200315 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448270 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200316 !Sample_title = CY89A_Core_15_A05_S5_comb !Sample_geo_accession = GSM3200316 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448269 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200317 !Sample_title = CY89COREQ1_C12_S324_comb !Sample_geo_accession = GSM3200317 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448268 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200318 !Sample_title = Cy81_Bulk_CD45_B08_S116_comb !Sample_geo_accession = GSM3200318 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448267 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200319 !Sample_title = CY89COREQ1_E10_S346_comb !Sample_geo_accession = GSM3200319 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448266 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200320 !Sample_title = cy80_CD45_neg_C10_S898_comb !Sample_geo_accession = GSM3200320 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448264 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200321 !Sample_title = cy53_1_CD45_pos_1_A02_S2_comb !Sample_geo_accession = GSM3200321 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448263 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200322 !Sample_title = cy58_1_CD45_pos_C04_S604_comb !Sample_geo_accession = GSM3200322 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448262 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200323 !Sample_title = cy53_1_CD45_neg_H07_S379_comb !Sample_geo_accession = GSM3200323 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448261 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200324 !Sample_title = Cy81_FNA_CD45_H05_S281_comb !Sample_geo_accession = GSM3200324 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448260 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200325 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_B11_S503_comb !Sample_geo_accession = GSM3200325 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448265 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200326 !Sample_title = cy82_CD45_pos_2_C04_S508_comb !Sample_geo_accession = GSM3200326 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448330 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200327 !Sample_title = cy82_CD45_pos_1_F01_S541_comb !Sample_geo_accession = GSM3200327 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448329 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200328 !Sample_title = cy53_1_CD45_pos_2_H12_S1056_comb !Sample_geo_accession = GSM3200328 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448328 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200329 !Sample_title = Cy72_CD45_A06_S678_comb !Sample_geo_accession = GSM3200329 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448327 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200330 !Sample_title = Cy72_CD45_F10_S742_comb !Sample_geo_accession = GSM3200330 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448326 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200331 !Sample_title = CY94CD45POS_1_E07_S151_comb !Sample_geo_accession = GSM3200331 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448325 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200332 !Sample_title = cy60_1_cd_45_pos_4_C09_S33_comb !Sample_geo_accession = GSM3200332 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448324 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200333 !Sample_title = cy80_CD_90_pos_B02_S878_comb !Sample_geo_accession = GSM3200333 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448323 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200334 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_G08_S560_comb !Sample_geo_accession = GSM3200334 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448322 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200335 !Sample_title = CY88CD45_150813_G11_S371_comb !Sample_geo_accession = GSM3200335 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448321 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200336 !Sample_title = CY89COREQ1_B10_S310_comb !Sample_geo_accession = GSM3200336 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448320 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200337 !Sample_title = cy60_1_cd_45_pos_4_H06_S90_comb !Sample_geo_accession = GSM3200337 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448319 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200338 !Sample_title = CY84_PRIM_POS_ALL_8_D04_S136_comb !Sample_geo_accession = GSM3200338 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448318 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200339 !Sample_title = CY88CD45POS_2_F01_S445_comb !Sample_geo_accession = GSM3200339 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448317 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200340 !Sample_title = Cy80_II_CD45_C12_S900_comb !Sample_geo_accession = GSM3200340 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448316 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200341 !Sample_title = Cy74_CD45_A02_S386_comb !Sample_geo_accession = GSM3200341 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448315 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200342 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_H08_S1052_comb !Sample_geo_accession = GSM3200342 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448314 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200343 !Sample_title = cy80_Cd45_pos_PD1_pos_D01_S85_comb !Sample_geo_accession = GSM3200343 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449500 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200344 !Sample_title = cy58_1_CD45_pos_B03_S591_comb !Sample_geo_accession = GSM3200344 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449499 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200345 !Sample_title = cy82_CD45_pos_1_D06_S522_comb !Sample_geo_accession = GSM3200345 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449498 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200346 !Sample_title = cy81_Bulk_CD45_neg_G02_S170_comb !Sample_geo_accession = GSM3200346 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449563 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200347 !Sample_title = cy80_CD45_neg_A04_S868_comb !Sample_geo_accession = GSM3200347 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449562 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200348 !Sample_title = CY89A_CD45_POS_6_C04_S124_comb !Sample_geo_accession = GSM3200348 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449561 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200349 !Sample_title = cy58_1_CD45_pos_A09_S585_comb !Sample_geo_accession = GSM3200349 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449560 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200350 !Sample_title = CY89A_Core_15_E11_S59_comb !Sample_geo_accession = GSM3200350 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449558 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200351 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_C05_S989_comb !Sample_geo_accession = GSM3200351 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449557 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200352 !Sample_title = CY88CD45_150813_A01_S289_comb !Sample_geo_accession = GSM3200352 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449556 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200353 !Sample_title = cy60_1_cd_45_pos_3_E11_S347_comb !Sample_geo_accession = GSM3200353 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449555 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200354 !Sample_title = cy60_1_cd_45_pos_4_C11_S35_comb !Sample_geo_accession = GSM3200354 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449554 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200355 !Sample_title = CY58_1_CD45_H08_S1052_comb !Sample_geo_accession = GSM3200355 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449553 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200356 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_H03_S1047_comb !Sample_geo_accession = GSM3200356 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449552 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200357 !Sample_title = cy60_1_cd_45_pos_4_E03_S51_comb !Sample_geo_accession = GSM3200357 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449551 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200358 !Sample_title = CY58_1_CD45_F08_S1028_comb !Sample_geo_accession = GSM3200358 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449550 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200359 !Sample_title = cy80_CD45_neg_E02_S434_comb !Sample_geo_accession = GSM3200359 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449549 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200360 !Sample_title = CY89FNAQ2_A03_S387_comb !Sample_geo_accession = GSM3200360 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449548 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200361 !Sample_title = cy58_1_CD45_pos_G11_S659_comb !Sample_geo_accession = GSM3200361 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449547 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200362 !Sample_title = CY89A_CD45_POS_6_D09_S141_comb !Sample_geo_accession = GSM3200362 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449546 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200363 !Sample_title = cy79_p3_CD45_pos_PD1_neg_A05_S101_comb !Sample_geo_accession = GSM3200363 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449545 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200364 !Sample_title = CY88_3_E09_S633_comb !Sample_geo_accession = GSM3200364 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449544 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200365 !Sample_title = Cy72_CD45_A03_S675_comb !Sample_geo_accession = GSM3200365 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449543 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200366 !Sample_title = cy80_Cd45_pos_Pd1_neg_S267_D03_S267_comb !Sample_geo_accession = GSM3200366 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449542 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200367 !Sample_title = CY89COREQ1_G11_S371_comb !Sample_geo_accession = GSM3200367 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449541 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200368 !Sample_title = Cy72_CD45_C03_S699_comb !Sample_geo_accession = GSM3200368 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449540 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200369 !Sample_title = cy80_CD45_neg_C08_S896_comb !Sample_geo_accession = GSM3200369 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449539 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200370 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_D10_S526_comb !Sample_geo_accession = GSM3200370 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449538 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200371 !Sample_title = Cy81_FNA_CD45_G11_S275_comb !Sample_geo_accession = GSM3200371 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449537 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200372 !Sample_title = cy94_cd45neg_cd90pos_E10_S346_comb !Sample_geo_accession = GSM3200372 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449536 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200373 !Sample_title = cy94_cd45pos_4_G06_S78_comb !Sample_geo_accession = GSM3200373 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448861 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200374 !Sample_title = cy94_cd45neg_cd90pos_F08_S356_comb !Sample_geo_accession = GSM3200374 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448860 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200375 !Sample_title = cy84_Met_CD45_pos_H09_S957_comb !Sample_geo_accession = GSM3200375 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448927 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200376 !Sample_title = cy78_CD45_neg_2_B11_S599_comb !Sample_geo_accession = GSM3200376 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448925 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200377 !Sample_title = Cy72_CD45_C06_S702_comb !Sample_geo_accession = GSM3200377 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448924 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200378 !Sample_title = cy53_1_CD45_neg_G06_S366_comb !Sample_geo_accession = GSM3200378 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448923 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200379 !Sample_title = cy82_CD45_pos_3_H01_S85_comb !Sample_geo_accession = GSM3200379 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448922 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200380 !Sample_title = Cy71_CD45_B04_S496_comb !Sample_geo_accession = GSM3200380 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448921 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200381 !Sample_title = Cy74_CD45_F10_S454_comb !Sample_geo_accession = GSM3200381 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448920 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200382 !Sample_title = cy88_cd45pos_5_B01_S205_comb !Sample_geo_accession = GSM3200382 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448919 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200383 !Sample_title = cy84_Primary_CD45_pos_A10_S394_comb !Sample_geo_accession = GSM3200383 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448918 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200384 !Sample_title = Cy81_Bulk_CD45_E11_S155_comb !Sample_geo_accession = GSM3200384 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448917 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200385 !Sample_title = cy60_1_cd_45_pos_4_A03_S3_comb !Sample_geo_accession = GSM3200385 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448916 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200386 !Sample_title = cy78_CD45_neg_3_A06_S678_comb !Sample_geo_accession = GSM3200386 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448915 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200387 !Sample_title = CY94CD45POS_1_C05_S125_comb !Sample_geo_accession = GSM3200387 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448914 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200388 !Sample_title = CY89A_Core_15_B09_S21_comb !Sample_geo_accession = GSM3200388 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448913 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200389 !Sample_title = CY89COREQ1_B08_S308_comb !Sample_geo_accession = GSM3200389 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448912 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200390 !Sample_title = cy82_CD45_pos_3_A07_S7_comb !Sample_geo_accession = GSM3200390 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448911 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200391 !Sample_title = CY89CORE11_E07_S151_comb !Sample_geo_accession = GSM3200391 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448910 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200392 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_A05_S677_comb !Sample_geo_accession = GSM3200392 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448909 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200393 !Sample_title = Cy80_II_CD45_G10_S946_comb !Sample_geo_accession = GSM3200393 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448908 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200394 !Sample_title = CY89NEG_D04_S40_comb !Sample_geo_accession = GSM3200394 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448907 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200395 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_G02_S266_comb !Sample_geo_accession = GSM3200395 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448906 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200396 !Sample_title = Cy80_II_CD45_F08_S932_comb !Sample_geo_accession = GSM3200396 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448905 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200397 !Sample_title = CY89A_Core_15_D11_S47_comb !Sample_geo_accession = GSM3200397 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448904 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200398 !Sample_title = cy53_1_CD45_pos_1_A08_S8_comb !Sample_geo_accession = GSM3200398 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448903 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200399 !Sample_title = CY89COREQ1_G07_S367_comb !Sample_geo_accession = GSM3200399 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448902 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200400 !Sample_title = cy80_Cd45_pos_Pd1_neg_S289_E01_S289_comb !Sample_geo_accession = GSM3200400 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448901 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200401 !Sample_title = CY88CD45_150813_E09_S345_comb !Sample_geo_accession = GSM3200401 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448900 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200402 !Sample_title = CY89FNA_A10_S202_comb !Sample_geo_accession = GSM3200402 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448899 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200403 !Sample_title = cy72_CD45_pos_G09_S945_comb !Sample_geo_accession = GSM3200403 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448799 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200404 !Sample_title = cy80_CD_90_pos_G06_S942_comb !Sample_geo_accession = GSM3200404 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448798 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200405 !Sample_title = Cy72_CD45_F04_S736_comb !Sample_geo_accession = GSM3200405 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448797 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200406 !Sample_title = cy79_p3_CD45_pos_PD1_neg_G10_S178_comb !Sample_geo_accession = GSM3200406 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448796 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200407 !Sample_title = cy94_cd45pos_G04_S172_comb !Sample_geo_accession = GSM3200407 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448795 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200408 !Sample_title = cy53_1_CD45_pos_2_C12_S996_comb !Sample_geo_accession = GSM3200408 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448794 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200409 !Sample_title = cy79_p3_CD45_pos_PD1_neg_E05_S149_comb !Sample_geo_accession = GSM3200409 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448793 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200410 !Sample_title = cy60_1_cd_45_pos_3_F07_S355_comb !Sample_geo_accession = GSM3200410 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448791 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200411 !Sample_title = cy53_1_CD45_pos_2_C10_S994_comb !Sample_geo_accession = GSM3200411 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448790 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200412 !Sample_title = CY89FNA_E03_S243_comb !Sample_geo_accession = GSM3200412 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448789 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200413 !Sample_title = cy60_1_cd_45_pos_4_D08_S44_comb !Sample_geo_accession = GSM3200413 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448788 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200414 !Sample_title = cy94_cd45pos_G05_S173_comb !Sample_geo_accession = GSM3200414 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448787 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200415 !Sample_title = cy94_cd45neg_cd90pos_G02_S362_comb !Sample_geo_accession = GSM3200415 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448792 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200416 !Sample_title = cy60_1_cd_45_pos_4_G04_S76_comb !Sample_geo_accession = GSM3200416 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448786 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200417 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_B07_S1075_comb !Sample_geo_accession = GSM3200417 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448785 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200418 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_G06_S270_comb !Sample_geo_accession = GSM3200418 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448784 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200419 !Sample_title = cy94_cd45neg_cd90pos_H01_S373_comb !Sample_geo_accession = GSM3200419 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448783 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200420 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_H06_S282_comb !Sample_geo_accession = GSM3200420 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448782 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200421 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_E09_S537_comb !Sample_geo_accession = GSM3200421 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448859 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200422 !Sample_title = cy80_cd_90_neg_C03_S123_comb !Sample_geo_accession = GSM3200422 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448858 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200423 !Sample_title = cy72_CD45_pos_D02_S902_comb !Sample_geo_accession = GSM3200423 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448857 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200424 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_A09_S9_comb !Sample_geo_accession = GSM3200424 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448856 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200425 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_G05_S1037_comb !Sample_geo_accession = GSM3200425 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448855 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200426 !Sample_title = CY88CD45_150813_G06_S366_comb !Sample_geo_accession = GSM3200426 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448854 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200427 !Sample_title = cy60_1_cd_45_pos_3_A06_S294_comb !Sample_geo_accession = GSM3200427 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448853 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200428 !Sample_title = CY88CD45POS_2_B09_S405_comb !Sample_geo_accession = GSM3200428 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448852 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200429 !Sample_title = CY89A_CD45_POS_10_B06_S210_comb !Sample_geo_accession = GSM3200429 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448851 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200430 !Sample_title = CY89FNA_C09_S225_comb !Sample_geo_accession = GSM3200430 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448850 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200431 !Sample_title = cy84_Primary_CD45_pos_C07_S415_comb !Sample_geo_accession = GSM3200431 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448849 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200432 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_C06_S510_comb !Sample_geo_accession = GSM3200432 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448848 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200433 !Sample_title = CY84_PRIM_POS_ALL_6_C05_S413_comb !Sample_geo_accession = GSM3200433 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447910 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200434 !Sample_title = cy60_1_cd_45_pos_3_G07_S367_comb !Sample_geo_accession = GSM3200434 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447909 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200435 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_H02_S1046_comb !Sample_geo_accession = GSM3200435 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447908 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200436 !Sample_title = cy78_CD45_neg_3_G01_S745_comb !Sample_geo_accession = GSM3200436 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447907 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200437 !Sample_title = cy82_CD45_pos_3_B12_S24_comb !Sample_geo_accession = GSM3200437 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447906 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200438 !Sample_title = cy94_cd45neg_cd90pos_D03_S327_comb !Sample_geo_accession = GSM3200438 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447905 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200439 !Sample_title = cy80_Cd45_pos_Pd1_neg_S343_G07_S343_comb !Sample_geo_accession = GSM3200439 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447904 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200440 !Sample_title = CY88CD45POS_2_D02_S422_comb !Sample_geo_accession = GSM3200440 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447902 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200441 !Sample_title = cy79_p4_CD45_neg_PDL1_pos_B08_S404_comb !Sample_geo_accession = GSM3200441 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447901 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200442 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_C04_S508_comb !Sample_geo_accession = GSM3200442 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447900 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200443 !Sample_title = CY84_PRIM_POS_ALL_6_C10_S418_comb !Sample_geo_accession = GSM3200443 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447899 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200444 !Sample_title = cy60_1_cd_45_pos_3_H06_S378_comb !Sample_geo_accession = GSM3200444 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447898 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200445 !Sample_title = cy80_CD_90_pos_E03_S915_comb !Sample_geo_accession = GSM3200445 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447903 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200446 !Sample_title = Cy71_CD45_F02_S542_comb !Sample_geo_accession = GSM3200446 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447897 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200447 !Sample_title = cy79_p3_CD45_pos_PD1_neg_B11_S119_comb !Sample_geo_accession = GSM3200447 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447896 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200448 !Sample_title = cy79_p3_CD45_pos_PD1_neg_A09_S105_comb !Sample_geo_accession = GSM3200448 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447895 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200449 !Sample_title = CY88CD45POS_7_B01_S205_comb !Sample_geo_accession = GSM3200449 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447894 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200450 !Sample_title = CY89A_Core_15_D01_S37_comb !Sample_geo_accession = GSM3200450 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447967 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200451 !Sample_title = CY89COREQ1_D04_S328_comb !Sample_geo_accession = GSM3200451 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447966 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200452 !Sample_title = CY94CD45POS_1_E09_S153_comb !Sample_geo_accession = GSM3200452 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447965 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200453 !Sample_title = CY58_1_CD45_C05_S989_comb !Sample_geo_accession = GSM3200453 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447964 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200454 !Sample_title = cy80_CD45_pos_PD1_pos_A04_S100_comb !Sample_geo_accession = GSM3200454 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447963 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200455 !Sample_title = cy80_Cd45_pos_Pd1_neg_S271_D07_S271_comb !Sample_geo_accession = GSM3200455 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447962 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200456 !Sample_title = cy80_Cd45_pos_Pd1_neg_S273_D09_S273_comb !Sample_geo_accession = GSM3200456 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447961 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200457 !Sample_title = cy72_CD45_pos_H08_S956_comb !Sample_geo_accession = GSM3200457 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447960 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200458 !Sample_title = CY88_5_H12_S768_comb !Sample_geo_accession = GSM3200458 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447959 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200459 !Sample_title = Cy72_CD45_G11_S755_comb !Sample_geo_accession = GSM3200459 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447958 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200460 !Sample_title = cy94_cd45pos_G01_S169_comb !Sample_geo_accession = GSM3200460 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447957 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200461 !Sample_title = cy94_cd45pos_4_G10_S82_comb !Sample_geo_accession = GSM3200461 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447956 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200462 !Sample_title = cy94_cd45pos_H01_S181_comb !Sample_geo_accession = GSM3200462 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447955 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200463 !Sample_title = cy60_1_cd_45_pos_4_B06_S18_comb !Sample_geo_accession = GSM3200463 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449535 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200464 !Sample_title = cy53_1_CD45_neg_F09_S357_comb !Sample_geo_accession = GSM3200464 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449534 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200465 !Sample_title = CY88CD45_150813_E05_S341_comb !Sample_geo_accession = GSM3200465 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449533 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200466 !Sample_title = cy80_Cd45_pos_Pd1_neg_S269_D05_S269_comb !Sample_geo_accession = GSM3200466 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449532 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200467 !Sample_title = cy60_1_cd_45_pos_4_H08_S92_comb !Sample_geo_accession = GSM3200467 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449531 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200468 !Sample_title = cy79_p3_CD45_pos_PD1_neg_G02_S170_comb !Sample_geo_accession = GSM3200468 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449530 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200469 !Sample_title = cy94_cd45pos_A03_S99_comb !Sample_geo_accession = GSM3200469 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449529 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200470 !Sample_title = cy84_Primary_CD45_pos_G09_S465_comb !Sample_geo_accession = GSM3200470 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449528 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200471 !Sample_title = CY89COREQ1_E07_S343_comb !Sample_geo_accession = GSM3200471 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449599 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200472 !Sample_title = cy80_Cd45_pos_Pd1_neg_S365_H05_S365_comb !Sample_geo_accession = GSM3200472 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449598 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200473 !Sample_title = CY84_PRIM_POS_ALL_8_H07_S187_comb !Sample_geo_accession = GSM3200473 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449597 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200474 !Sample_title = CY88CD45_150813_A12_S300_comb !Sample_geo_accession = GSM3200474 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449596 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200475 !Sample_title = cy84_Primary_CD45_pos_H01_S469_comb !Sample_geo_accession = GSM3200475 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449595 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200476 !Sample_title = CY94CD45POS_1_C02_S122_comb !Sample_geo_accession = GSM3200476 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449594 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200477 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_G10_S562_comb !Sample_geo_accession = GSM3200477 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449593 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200478 !Sample_title = cy80_CD_90_pos_D03_S903_comb !Sample_geo_accession = GSM3200478 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449592 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200479 !Sample_title = cy53_1_CD45_pos_2_G09_S1041_comb !Sample_geo_accession = GSM3200479 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449591 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200480 !Sample_title = CY88CD45POS_2_D04_S424_comb !Sample_geo_accession = GSM3200480 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449590 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200481 !Sample_title = cy94_cd45neg_cd90pos_D07_S331_comb !Sample_geo_accession = GSM3200481 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449589 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200482 !Sample_title = cy53_1_CD45_pos_1_E01_S49_comb !Sample_geo_accession = GSM3200482 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449588 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200483 !Sample_title = CY89CORE11_G06_S174_comb !Sample_geo_accession = GSM3200483 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449587 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200484 !Sample_title = CY89A_CD45_POS_6_A02_S98_comb !Sample_geo_accession = GSM3200484 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449586 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200485 !Sample_title = cy94_cd45neg_cd90pos_C11_S323_comb !Sample_geo_accession = GSM3200485 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449585 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200486 !Sample_title = CY89COREQ1_B06_S306_comb !Sample_geo_accession = GSM3200486 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449584 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200487 !Sample_title = cy84_Met_CD45_pos_B04_S880_comb !Sample_geo_accession = GSM3200487 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449583 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200488 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_E11_S251_comb !Sample_geo_accession = GSM3200488 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449582 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200489 !Sample_title = CY58_1_CD45_B10_S982_comb !Sample_geo_accession = GSM3200489 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449581 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200490 !Sample_title = CY84_PRIM_POS_ALL_6_G08_S464_comb !Sample_geo_accession = GSM3200490 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449580 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200491 !Sample_title = cy94_cd45pos_4_D04_S40_comb !Sample_geo_accession = GSM3200491 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449579 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200492 !Sample_title = cy60_1_cd_45_pos_3_A10_S298_comb !Sample_geo_accession = GSM3200492 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449578 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200493 !Sample_title = CY89FNA_H07_S283_comb !Sample_geo_accession = GSM3200493 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449102 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200494 !Sample_title = CY88_5_E01_S721_comb !Sample_geo_accession = GSM3200494 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449101 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200495 !Sample_title = cy79_p3_CD45_pos_PD1_neg_G03_S171_comb !Sample_geo_accession = GSM3200495 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449100 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200496 !Sample_title = cy72_CD45_pos_D09_S909_comb !Sample_geo_accession = GSM3200496 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449099 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200497 !Sample_title = cy72_CD45_pos_B01_S877_comb !Sample_geo_accession = GSM3200497 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449098 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200498 !Sample_title = cy80_Cd45_pos_Pd1_neg_S341_G05_S341_comb !Sample_geo_accession = GSM3200498 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449097 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200499 !Sample_title = cy74_CD45_pos_A08_S680_comb !Sample_geo_accession = GSM3200499 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449094 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200500 !Sample_title = cy60_1_cd_45_pos_3_D04_S328_comb !Sample_geo_accession = GSM3200500 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449093 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200501 !Sample_title = cy79_p3_CD45_pos_PD1_neg_G09_S177_comb !Sample_geo_accession = GSM3200501 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449092 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200502 !Sample_title = CY88CD45_150813_C01_S313_comb !Sample_geo_accession = GSM3200502 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449091 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200503 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_C09_S993_comb !Sample_geo_accession = GSM3200503 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449090 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200504 !Sample_title = CY89COREQ1_H01_S373_comb !Sample_geo_accession = GSM3200504 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449089 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200505 !Sample_title = cy72_CD45_pos_E10_S922_comb !Sample_geo_accession = GSM3200505 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449095 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200506 !Sample_title = CY88CD45POS_7_F02_S254_comb !Sample_geo_accession = GSM3200506 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449088 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200507 !Sample_title = CY89NEG_B06_S18_comb !Sample_geo_accession = GSM3200507 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449087 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200508 !Sample_title = cy80_Cd45_pos_Pd1_neg_S200_A08_S200_comb !Sample_geo_accession = GSM3200508 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449086 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200509 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_H11_S1055_comb !Sample_geo_accession = GSM3200509 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449085 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200510 !Sample_title = CY58_1_CD45_A05_S965_comb !Sample_geo_accession = GSM3200510 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449084 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200511 !Sample_title = CY84_PRIM_POS_ALL_7_H06_S282_comb !Sample_geo_accession = GSM3200511 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449083 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200512 !Sample_title = cy84_Primary_CD45_pos_A11_S395_comb !Sample_geo_accession = GSM3200512 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449082 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200513 !Sample_title = cy60_1_cd_45_pos_3_D09_S333_comb !Sample_geo_accession = GSM3200513 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449081 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200514 !Sample_title = cy80_cd_90_neg_G12_S180_comb !Sample_geo_accession = GSM3200514 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449080 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200515 !Sample_title = cy81_Bulk_CD45_neg_E03_S147_comb !Sample_geo_accession = GSM3200515 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449079 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200516 !Sample_title = cy53_1_CD45_neg_B05_S305_comb !Sample_geo_accession = GSM3200516 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449078 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200517 !Sample_title = Cy81_FNA_CD45_C01_S217_comb !Sample_geo_accession = GSM3200517 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449077 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200518 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_B03_S687_comb !Sample_geo_accession = GSM3200518 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449076 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200519 !Sample_title = CY89A_CD45_POS_6_H09_S189_comb !Sample_geo_accession = GSM3200519 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449075 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200520 !Sample_title = cy74_CD45_pos_G10_S754_comb !Sample_geo_accession = GSM3200520 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449074 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200521 !Sample_title = cy72_CD45_pos_F02_S926_comb !Sample_geo_accession = GSM3200521 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449073 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200522 !Sample_title = Cy81_FNA_CD45_D01_S229_comb !Sample_geo_accession = GSM3200522 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449072 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200523 !Sample_title = cy94_cd45neg_cd90pos_B03_S303_comb !Sample_geo_accession = GSM3200523 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449494 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200524 !Sample_title = CY94CD45POS_1_E06_S150_comb !Sample_geo_accession = GSM3200524 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449493 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200525 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_F08_S1028_comb !Sample_geo_accession = GSM3200525 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449492 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200526 !Sample_title = cy53_1_CD45_pos_1_A03_S3_comb !Sample_geo_accession = GSM3200526 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449491 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200527 !Sample_title = cy79_p3_CD45_pos_PD1_neg_G06_S174_comb !Sample_geo_accession = GSM3200527 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449490 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200528 !Sample_title = cy80_CD_90_pos_B06_S882_comb !Sample_geo_accession = GSM3200528 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449489 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200529 !Sample_title = cy94_cd45pos_4_H01_S85_comb !Sample_geo_accession = GSM3200529 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449488 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200530 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_B03_S975_comb !Sample_geo_accession = GSM3200530 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449487 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200531 !Sample_title = cy94_cd45neg_cd90pos_H08_S380_comb !Sample_geo_accession = GSM3200531 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449484 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200532 !Sample_title = cy79_p1_CD45_neg_PDL1_pos_AS_C1_R1_E09_S57_comb !Sample_geo_accession = GSM3200532 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449483 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200533 !Sample_title = cy80_CD45_neg_A06_S390_comb !Sample_geo_accession = GSM3200533 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449482 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200534 !Sample_title = cy53_1_CD45_neg_B04_S304_comb !Sample_geo_accession = GSM3200534 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449481 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200535 !Sample_title = CY84_PRIM_POS_ALL_8_G07_S175_comb !Sample_geo_accession = GSM3200535 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449486 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200536 !Sample_title = cy94_cd45pos_4_B12_S24_comb !Sample_geo_accession = GSM3200536 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449485 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200537 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_F04_S832_comb !Sample_geo_accession = GSM3200537 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449480 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200538 !Sample_title = cy94_cd45neg_cd90pos_F02_S350_comb !Sample_geo_accession = GSM3200538 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449479 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200539 !Sample_title = Cy74_CD45_A06_S390_comb !Sample_geo_accession = GSM3200539 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449478 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200540 !Sample_title = cy84_Primary_CD45_pos_D01_S421_comb !Sample_geo_accession = GSM3200540 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449477 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200541 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_C04_S988_comb !Sample_geo_accession = GSM3200541 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449476 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200542 !Sample_title = cy88_cd_45_pos_F02_S446_comb !Sample_geo_accession = GSM3200542 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449475 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200543 !Sample_title = cy82_CD45_neg_1_D01_S229_comb !Sample_geo_accession = GSM3200543 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449474 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200544 !Sample_title = cy80_Cd45_pos_Pd1_neg_S346_G10_S346_comb !Sample_geo_accession = GSM3200544 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449473 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200545 !Sample_title = Cy71_CD45_H12_S672_comb !Sample_geo_accession = GSM3200545 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449472 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200546 !Sample_title = cy94_cd45pos_D10_S142_comb !Sample_geo_accession = GSM3200546 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449471 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200547 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_B02_S974_comb !Sample_geo_accession = GSM3200547 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449470 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200548 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_G08_S1040_comb !Sample_geo_accession = GSM3200548 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449469 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200549 !Sample_title = cy80_Cd45_pos_Pd1_neg_S252_C12_S252_comb !Sample_geo_accession = GSM3200549 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449468 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200550 !Sample_title = CY89A_CD45_POS_6_A05_S101_comb !Sample_geo_accession = GSM3200550 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449467 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200551 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_F05_S1025_comb !Sample_geo_accession = GSM3200551 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449466 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200552 !Sample_title = cy94_cd45pos_4_E09_S57_comb !Sample_geo_accession = GSM3200552 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449465 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200553 !Sample_title = cy60_1_cd_45_pos_3_G08_S368_comb !Sample_geo_accession = GSM3200553 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449464 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200554 !Sample_title = Cy81_Bulk_CD45_E07_S151_comb !Sample_geo_accession = GSM3200554 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449463 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200555 !Sample_title = cy60_1_cd_45_pos_4_C08_S32_comb !Sample_geo_accession = GSM3200555 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449462 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200556 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_H07_S571_comb !Sample_geo_accession = GSM3200556 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449461 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200557 !Sample_title = cy74_CD45_pos_G04_S748_comb !Sample_geo_accession = GSM3200557 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449527 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200558 !Sample_title = cy74_CD45_pos_B10_S694_comb !Sample_geo_accession = GSM3200558 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449526 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200559 !Sample_title = Cy81_Bulk_CD45_F07_S163_comb !Sample_geo_accession = GSM3200559 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449525 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200560 !Sample_title = cy72_CD45_pos_D04_S904_comb !Sample_geo_accession = GSM3200560 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449524 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200561 !Sample_title = cy94_cd45pos_H07_S187_comb !Sample_geo_accession = GSM3200561 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449522 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200562 !Sample_title = Cy72_CD45_D06_S714_comb !Sample_geo_accession = GSM3200562 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449521 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200563 !Sample_title = cy82_CD45_pos_3_B08_S20_comb !Sample_geo_accession = GSM3200563 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449520 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200564 !Sample_title = CY58_1_CD45_G12_S1044_comb !Sample_geo_accession = GSM3200564 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449519 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200565 !Sample_title = CY89A_CD45_POS_6_A06_S102_comb !Sample_geo_accession = GSM3200565 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449518 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200566 !Sample_title = Cy74_CD45_C07_S415_comb !Sample_geo_accession = GSM3200566 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449517 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200567 !Sample_title = cy94_cd45pos_B10_S118_comb !Sample_geo_accession = GSM3200567 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449516 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200568 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_B04_S976_comb !Sample_geo_accession = GSM3200568 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449515 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200569 !Sample_title = cy60_1_cd_45_pos_4_G11_S83_comb !Sample_geo_accession = GSM3200569 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449514 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200570 !Sample_title = cy94_cd45pos_4_C07_S31_comb !Sample_geo_accession = GSM3200570 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449513 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200571 !Sample_title = cy60_1_cd_45_pos_3_A07_S295_comb !Sample_geo_accession = GSM3200571 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449512 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200572 !Sample_title = cy72_CD45_pos_G01_S937_comb !Sample_geo_accession = GSM3200572 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449511 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200573 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_D03_S519_comb !Sample_geo_accession = GSM3200573 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449510 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200574 !Sample_title = CY89A_CD45_POS_10_E06_S246_comb !Sample_geo_accession = GSM3200574 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449509 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200575 !Sample_title = Cy72_CD45_G08_S752_comb !Sample_geo_accession = GSM3200575 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449508 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200576 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_D07_S523_comb !Sample_geo_accession = GSM3200576 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449507 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200577 !Sample_title = cy80_Cd45_pos_Pd1_neg_S292_E04_S292_comb !Sample_geo_accession = GSM3200577 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449506 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200578 !Sample_title = cy84_Met_CD45_pos_A02_S866_comb !Sample_geo_accession = GSM3200578 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449505 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200579 !Sample_title = cy80_Cd45_pos_Pd1_neg_S199_A07_S199_comb !Sample_geo_accession = GSM3200579 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449504 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200580 !Sample_title = Cy81_Bulk_CD45_C07_S127_comb !Sample_geo_accession = GSM3200580 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449503 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200581 !Sample_title = Cy72_CD45_D02_S710_comb !Sample_geo_accession = GSM3200581 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449502 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200582 !Sample_title = cy94_cd45pos_D03_S135_comb !Sample_geo_accession = GSM3200582 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449501 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200583 !Sample_title = cy60_1_cd_45_pos_4_C07_S31_comb !Sample_geo_accession = GSM3200583 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448474 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200584 !Sample_title = CY89A_Core_15_E06_S54_comb !Sample_geo_accession = GSM3200584 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448473 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200585 !Sample_title = cy53_1_CD45_pos_2_A11_S971_comb !Sample_geo_accession = GSM3200585 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448543 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200586 !Sample_title = CY89A_Core_15_B08_S20_comb !Sample_geo_accession = GSM3200586 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448542 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200587 !Sample_title = cy94_cd45neg_cd90pos_F10_S358_comb !Sample_geo_accession = GSM3200587 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448541 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200588 !Sample_title = CY89A_CD45_POS_6_H10_S190_comb !Sample_geo_accession = GSM3200588 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448540 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200589 !Sample_title = CY88CD45_150813_C07_S319_comb !Sample_geo_accession = GSM3200589 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448539 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200590 !Sample_title = cy94_cd45neg_cd90pos_A08_S296_comb !Sample_geo_accession = GSM3200590 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448538 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200591 !Sample_title = cy72_CD45_pos_D12_S912_comb !Sample_geo_accession = GSM3200591 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448537 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200592 !Sample_title = CY89A_CD45_POS_6_G12_S180_comb !Sample_geo_accession = GSM3200592 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448536 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200593 !Sample_title = cy60_1_cd_45_pos_4_G09_S81_comb !Sample_geo_accession = GSM3200593 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448535 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200594 !Sample_title = cy94_cd45pos_G07_S175_comb !Sample_geo_accession = GSM3200594 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448534 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200595 !Sample_title = cy58_1_CD45_pos_C05_S605_comb !Sample_geo_accession = GSM3200595 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448533 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200596 !Sample_title = CY89A_Core_15_G09_S81_comb !Sample_geo_accession = GSM3200596 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448532 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200597 !Sample_title = CY58_1_CD45_A11_S971_comb !Sample_geo_accession = GSM3200597 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448531 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200598 !Sample_title = cy94_cd45pos_4_C08_S32_comb !Sample_geo_accession = GSM3200598 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448530 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200599 !Sample_title = CY88CD45POS_2_H08_S476_comb !Sample_geo_accession = GSM3200599 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448529 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200600 !Sample_title = Cy81_Bulk_CD45_F08_S164_comb !Sample_geo_accession = GSM3200600 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448528 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200601 !Sample_title = Cy80_II_CD45_C03_S891_comb !Sample_geo_accession = GSM3200601 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448527 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200602 !Sample_title = cy82_CD45_neg_3_D03_S807_comb !Sample_geo_accession = GSM3200602 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448526 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200603 !Sample_title = cy60_1_cd_45_neg_1_D12_S240_comb !Sample_geo_accession = GSM3200603 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448525 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200604 !Sample_title = cy94_cd45pos_4_E03_S51_comb !Sample_geo_accession = GSM3200604 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448524 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200605 !Sample_title = CY94CD45POS_1_H12_S192_comb !Sample_geo_accession = GSM3200605 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448714 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200606 !Sample_title = CY84_PRIM_POS_ALL_7_A12_S204_comb !Sample_geo_accession = GSM3200606 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448713 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200607 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_C10_S514_comb !Sample_geo_accession = GSM3200607 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448712 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200608 !Sample_title = cy80_CD45_neg_E01_S433_comb !Sample_geo_accession = GSM3200608 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448711 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200609 !Sample_title = cy80_CD_90_pos_E05_S917_comb !Sample_geo_accession = GSM3200609 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448710 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200610 !Sample_title = cy94_cd45pos_D11_S143_comb !Sample_geo_accession = GSM3200610 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448781 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200611 !Sample_title = CY84_PRIM_POS_ALL_6_A11_S395_comb !Sample_geo_accession = GSM3200611 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448780 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200612 !Sample_title = CY89A_Core_15_F06_S66_comb !Sample_geo_accession = GSM3200612 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448779 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200613 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_B11_S983_comb !Sample_geo_accession = GSM3200613 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448778 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200614 !Sample_title = Cy72_CD45_E09_S729_comb !Sample_geo_accession = GSM3200614 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448777 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200615 !Sample_title = cy72_CD45_pos_G04_S940_comb !Sample_geo_accession = GSM3200615 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448776 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200616 !Sample_title = CY88CD45POS_2_G11_S467_comb !Sample_geo_accession = GSM3200616 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448775 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200617 !Sample_title = cy72_CD45_pos_E02_S914_comb !Sample_geo_accession = GSM3200617 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448774 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200618 !Sample_title = cy78_CD45_neg_3_G05_S749_comb !Sample_geo_accession = GSM3200618 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448773 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200619 !Sample_title = cy79_p3_CD45_pos_PD1_neg_C10_S130_comb !Sample_geo_accession = GSM3200619 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448772 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200620 !Sample_title = Cy72_CD45_B03_S687_comb !Sample_geo_accession = GSM3200620 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448771 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200621 !Sample_title = CY88CD45_150813_H04_S376_comb !Sample_geo_accession = GSM3200621 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448770 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200622 !Sample_title = cy84_Met_CD45_pos_F10_S934_comb !Sample_geo_accession = GSM3200622 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448769 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200623 !Sample_title = cy60_1_cd_45_pos_3_D02_S326_comb !Sample_geo_accession = GSM3200623 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448768 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200624 !Sample_title = cy79_p3_CD45_pos_PD1_neg_F08_S164_comb !Sample_geo_accession = GSM3200624 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448767 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200625 !Sample_title = CY94CD45POS_1_C06_S126_comb !Sample_geo_accession = GSM3200625 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448766 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200626 !Sample_title = CY88CD45POS_2_H10_S478_comb !Sample_geo_accession = GSM3200626 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448765 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200627 !Sample_title = cy88_cd_45_pos_F05_S449_comb !Sample_geo_accession = GSM3200627 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448764 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200628 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_B10_S502_comb !Sample_geo_accession = GSM3200628 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448763 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200629 !Sample_title = Cy81_Bulk_CD45_E09_S153_comb !Sample_geo_accession = GSM3200629 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448762 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200630 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_G02_S1034_comb !Sample_geo_accession = GSM3200630 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448761 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200631 !Sample_title = Cy81_FNA_CD45_F12_S264_comb !Sample_geo_accession = GSM3200631 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448760 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200632 !Sample_title = cy88_cd_45_pos_3_F05_S641_comb !Sample_geo_accession = GSM3200632 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448759 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200633 !Sample_title = Cy80_II_CD45_A04_S868_comb !Sample_geo_accession = GSM3200633 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448758 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200634 !Sample_title = cy94_cd45pos_F11_S167_comb !Sample_geo_accession = GSM3200634 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448757 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200635 !Sample_title = cy53_1_CD45_pos_2_D09_S1005_comb !Sample_geo_accession = GSM3200635 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448756 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200636 !Sample_title = CY84_PRIM_POS_ALL_7_F11_S263_comb !Sample_geo_accession = GSM3200636 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448755 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200637 !Sample_title = CY89A_CD45_POS_6_F07_S163_comb !Sample_geo_accession = GSM3200637 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448754 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200638 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_C08_S512_comb !Sample_geo_accession = GSM3200638 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448753 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200639 !Sample_title = CY94CD45POS_1_A10_S106_comb !Sample_geo_accession = GSM3200639 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448752 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200640 !Sample_title = CY89A_CD45_POS_6_D01_S133_comb !Sample_geo_accession = GSM3200640 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448751 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200641 !Sample_title = cy84_Primary_CD45_pos_D02_S422_comb !Sample_geo_accession = GSM3200641 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448750 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200642 !Sample_title = CY88CD45_150813_C06_S318_comb !Sample_geo_accession = GSM3200642 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448749 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200643 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_E05_S533_comb !Sample_geo_accession = GSM3200643 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448748 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200644 !Sample_title = CY89A_CD45_POS_6_C12_S132_comb !Sample_geo_accession = GSM3200644 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448747 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200645 !Sample_title = cy84_Primary_CD45_pos_A03_S387_comb !Sample_geo_accession = GSM3200645 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448746 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200646 !Sample_title = CY89A_CD45_POS_6_D08_S140_comb !Sample_geo_accession = GSM3200646 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448745 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200647 !Sample_title = cy84_Primary_CD45_pos_C09_S417_comb !Sample_geo_accession = GSM3200647 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448817 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200648 !Sample_title = CY94CD45POS_1_E05_S149_comb !Sample_geo_accession = GSM3200648 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448816 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200649 !Sample_title = CY88CD45POS_7_F03_S255_comb !Sample_geo_accession = GSM3200649 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448815 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200650 !Sample_title = cy80_CD45_neg_H01_S469_comb !Sample_geo_accession = GSM3200650 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448814 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200651 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_G11_S563_comb !Sample_geo_accession = GSM3200651 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448813 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200652 !Sample_title = cy82_CD45_pos_3_G11_S83_comb !Sample_geo_accession = GSM3200652 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448812 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200653 !Sample_title = cy88_cd_45_pos_D01_S421_comb !Sample_geo_accession = GSM3200653 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448811 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200654 !Sample_title = Cy72_CD45_F01_S733_comb !Sample_geo_accession = GSM3200654 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448810 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200655 !Sample_title = CY94CD45POS_1_F12_S168_comb !Sample_geo_accession = GSM3200655 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448809 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200656 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_E02_S1010_comb !Sample_geo_accession = GSM3200656 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448808 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200657 !Sample_title = Cy74_CD45_B02_S398_comb !Sample_geo_accession = GSM3200657 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448807 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200658 !Sample_title = cy94_cd45pos_H08_S188_comb !Sample_geo_accession = GSM3200658 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448806 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200659 !Sample_title = CY84_PRIM_POS_ALL_6_H12_S480_comb !Sample_geo_accession = GSM3200659 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448805 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200660 !Sample_title = cy53_1_CD45_pos_2_H09_S1053_comb !Sample_geo_accession = GSM3200660 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448804 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200661 !Sample_title = CY89A_Core_15_A04_S4_comb !Sample_geo_accession = GSM3200661 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448803 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200662 !Sample_title = cy94_cd45pos_4_G12_S84_comb !Sample_geo_accession = GSM3200662 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448802 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200663 !Sample_title = cy60_1_cd_45_pos_3_B02_S302_comb !Sample_geo_accession = GSM3200663 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448801 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200664 !Sample_title = CY88CD45_150813_G08_S368_comb !Sample_geo_accession = GSM3200664 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448800 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200665 !Sample_title = cy84_Primary_CD45_pos_B05_S401_comb !Sample_geo_accession = GSM3200665 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447858 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200666 !Sample_title = CY94CD45POS_1_F09_S165_comb !Sample_geo_accession = GSM3200666 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447857 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200667 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_G08_S272_comb !Sample_geo_accession = GSM3200667 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447856 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200668 !Sample_title = cy84_Primary_CD45_pos_E10_S442_comb !Sample_geo_accession = GSM3200668 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447855 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200669 !Sample_title = cy80_Cd45_pos_Pd1_neg_S372_H12_S372_comb !Sample_geo_accession = GSM3200669 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447854 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200670 !Sample_title = CY94CD45POS_1_H04_S184_comb !Sample_geo_accession = GSM3200670 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447853 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200671 !Sample_title = CY88CD45POS_2_H09_S477_comb !Sample_geo_accession = GSM3200671 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447852 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200672 !Sample_title = cy94_cd45neg_cd90pos_E04_S340_comb !Sample_geo_accession = GSM3200672 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447851 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200673 !Sample_title = CY94CD45POS_1_H06_S186_comb !Sample_geo_accession = GSM3200673 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447850 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200674 !Sample_title = CY89A_CD45_POS_10_A03_S195_comb !Sample_geo_accession = GSM3200674 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447849 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200675 !Sample_title = CY58_1_CD45_C12_S996_comb !Sample_geo_accession = GSM3200675 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447848 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200676 !Sample_title = cy53_1_CD45_neg_E03_S339_comb !Sample_geo_accession = GSM3200676 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447847 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200677 !Sample_title = cy94_cd45pos_4_G03_S75_comb !Sample_geo_accession = GSM3200677 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447846 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200678 !Sample_title = CY94CD45POS_1_G05_S173_comb !Sample_geo_accession = GSM3200678 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447845 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200679 !Sample_title = CY89A_Core_15_C03_S27_comb !Sample_geo_accession = GSM3200679 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447844 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200680 !Sample_title = cy82_CD45_neg_3_C08_S800_comb !Sample_geo_accession = GSM3200680 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447843 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200681 !Sample_title = cy53_1_CD45_pos_2_D10_S1006_comb !Sample_geo_accession = GSM3200681 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447842 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200682 !Sample_title = cy79_p3_CD45_pos_PD1_neg_E10_S154_comb !Sample_geo_accession = GSM3200682 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447841 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200683 !Sample_title = cy53_1_CD45_pos_1_B02_S14_comb !Sample_geo_accession = GSM3200683 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447840 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200684 !Sample_title = CY94CD45POS_1_E10_S154_comb !Sample_geo_accession = GSM3200684 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447839 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200685 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_A08_S968_comb !Sample_geo_accession = GSM3200685 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447838 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200686 !Sample_title = CY94CD45POS_1_A04_S100_comb !Sample_geo_accession = GSM3200686 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447837 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200687 !Sample_title = cy78_CD45_neg_1_H12_S576_comb !Sample_geo_accession = GSM3200687 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447836 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200688 !Sample_title = CY88CD45_150813_B11_S311_comb !Sample_geo_accession = GSM3200688 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447835 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200689 !Sample_title = CY94CD45POS_1_D06_S138_comb !Sample_geo_accession = GSM3200689 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447834 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200690 !Sample_title = CY88CD45_150813_F05_S353_comb !Sample_geo_accession = GSM3200690 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447833 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200691 !Sample_title = Cy72_CD45_H01_S757_comb !Sample_geo_accession = GSM3200691 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447832 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200692 !Sample_title = cy60_1_cd_45_pos_4_H12_S96_comb !Sample_geo_accession = GSM3200692 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447831 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200693 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_F03_S543_comb !Sample_geo_accession = GSM3200693 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447830 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200694 !Sample_title = cy94_cd45neg_cd90pos_A07_S295_comb !Sample_geo_accession = GSM3200694 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447829 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200695 !Sample_title = cy60_1_cd_45_pos_4_A04_S4_comb !Sample_geo_accession = GSM3200695 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449321 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200696 !Sample_title = cy94_cd45neg_cd90pos_F09_S357_comb !Sample_geo_accession = GSM3200696 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449320 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200697 !Sample_title = CY89A_CD45_POS_10_H11_S287_comb !Sample_geo_accession = GSM3200697 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449319 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200698 !Sample_title = cy58_1_CD45_pos_D11_S623_comb !Sample_geo_accession = GSM3200698 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449318 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200699 !Sample_title = cy60_1_cd_45_pos_3_G10_S370_comb !Sample_geo_accession = GSM3200699 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449317 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200700 !Sample_title = cy84_Met_CD45_pos_A01_S865_comb !Sample_geo_accession = GSM3200700 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449316 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200701 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_D05_S1001_comb !Sample_geo_accession = GSM3200701 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449315 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200702 !Sample_title = cy80_CD45_neg_B04_S400_comb !Sample_geo_accession = GSM3200702 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449314 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200703 !Sample_title = cy94_cd45neg_cd90pos_D12_S336_comb !Sample_geo_accession = GSM3200703 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449313 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200704 !Sample_title = CY94CD45POS_1_G08_S176_comb !Sample_geo_accession = GSM3200704 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449312 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200705 !Sample_title = CY89A_CD45_POS_6_C03_S123_comb !Sample_geo_accession = GSM3200705 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449311 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200706 !Sample_title = Cy71_CD45_F09_S549_comb !Sample_geo_accession = GSM3200706 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449310 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200707 !Sample_title = cy80_CD45_neg_F07_S931_comb !Sample_geo_accession = GSM3200707 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449309 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200708 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_G07_S1039_comb !Sample_geo_accession = GSM3200708 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449308 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200709 !Sample_title = cy94_cd45neg_cd90pos_D11_S335_comb !Sample_geo_accession = GSM3200709 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449307 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200710 !Sample_title = cy79_p5_CD45_neg_PDL1_neg_B12_S792_comb !Sample_geo_accession = GSM3200710 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449306 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200711 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_E12_S540_comb !Sample_geo_accession = GSM3200711 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449305 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200712 !Sample_title = Cy81_FNA_CD45_H10_S286_comb !Sample_geo_accession = GSM3200712 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449304 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200713 !Sample_title = cy79_p4_CD45_pos_PD1_pos_C08_S320_comb !Sample_geo_accession = GSM3200713 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449303 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200714 !Sample_title = CY88CD45_150813_G12_S372_comb !Sample_geo_accession = GSM3200714 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449297 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200715 !Sample_title = CY88CD45POS_2_F07_S451_comb !Sample_geo_accession = GSM3200715 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449302 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200716 !Sample_title = cy94_cd45neg_cd90pos_H09_S381_comb !Sample_geo_accession = GSM3200716 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449301 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200717 !Sample_title = CY94CD45POS_1_B01_S109_comb !Sample_geo_accession = GSM3200717 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449300 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200718 !Sample_title = Cy71_CD45_A09_S489_comb !Sample_geo_accession = GSM3200718 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449299 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200719 !Sample_title = cy53_1_CD45_pos_2_D03_S999_comb !Sample_geo_accession = GSM3200719 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449298 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200720 !Sample_title = cy94_cd45neg_cd90pos_A06_S294_comb !Sample_geo_accession = GSM3200720 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449296 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200721 !Sample_title = cy53_1_CD45_pos_2_F03_S1023_comb !Sample_geo_accession = GSM3200721 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449295 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200722 !Sample_title = cy84_Primary_CD45_neg_A12_S972_comb !Sample_geo_accession = GSM3200722 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449294 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200723 !Sample_title = cy94_cd45neg_cd90pos_D04_S328_comb !Sample_geo_accession = GSM3200723 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449293 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200724 !Sample_title = cy94_cd45pos_4_H06_S90_comb !Sample_geo_accession = GSM3200724 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449292 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200725 !Sample_title = cy80_cd_90_neg_H12_S192_comb !Sample_geo_accession = GSM3200725 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447828 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200726 !Sample_title = cy94_cd45pos_4_B09_S21_comb !Sample_geo_accession = GSM3200726 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447827 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200727 !Sample_title = Cy71_CD45_H03_S663_comb !Sample_geo_accession = GSM3200727 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447826 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200728 !Sample_title = cy88_cd_45_pos_H12_S480_comb !Sample_geo_accession = GSM3200728 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447825 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200729 !Sample_title = CY89A_CD45_POS_6_A10_S106_comb !Sample_geo_accession = GSM3200729 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447824 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200730 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_E06_S246_comb !Sample_geo_accession = GSM3200730 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447893 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200731 !Sample_title = cy82_CD45_pos_3_D04_S40_comb !Sample_geo_accession = GSM3200731 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447892 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200732 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_E03_S531_comb !Sample_geo_accession = GSM3200732 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447891 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200733 !Sample_title = cy94_cd45neg_cd90pos_H03_S375_comb !Sample_geo_accession = GSM3200733 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447890 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200734 !Sample_title = cy94_cd45pos_F12_S168_comb !Sample_geo_accession = GSM3200734 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447889 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200735 !Sample_title = cy72_CD45_pos_C11_S899_comb !Sample_geo_accession = GSM3200735 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447888 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200736 !Sample_title = cy94_cd45pos_C01_S121_comb !Sample_geo_accession = GSM3200736 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447887 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200737 !Sample_title = cy94_cd45neg_cd90pos_F05_S353_comb !Sample_geo_accession = GSM3200737 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447886 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200738 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_H10_S1054_comb !Sample_geo_accession = GSM3200738 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447885 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200739 !Sample_title = cy94_cd45neg_cd90pos_D09_S333_comb !Sample_geo_accession = GSM3200739 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447884 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200740 !Sample_title = CY89COREQ1_G06_S366_comb !Sample_geo_accession = GSM3200740 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447883 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200741 !Sample_title = cy94_cd45neg_cd90pos_F12_S360_comb !Sample_geo_accession = GSM3200741 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447882 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200742 !Sample_title = CY89A_CD45_POS_6_F03_S159_comb !Sample_geo_accession = GSM3200742 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447881 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200743 !Sample_title = cy53_1_CD45_pos_2_A06_S966_comb !Sample_geo_accession = GSM3200743 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447880 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200744 !Sample_title = cy84_Primary_CD45_pos_G03_S459_comb !Sample_geo_accession = GSM3200744 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447879 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200745 !Sample_title = cy79_p3_CD45_pos_PD1_neg_A04_S100_comb !Sample_geo_accession = GSM3200745 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447878 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200746 !Sample_title = Cy74_CD45_H10_S478_comb !Sample_geo_accession = GSM3200746 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447877 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200747 !Sample_title = CY89A_Core_15_D06_S42_comb !Sample_geo_accession = GSM3200747 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447876 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200748 !Sample_title = cy60_1_cd_45_pos_4_B02_S14_comb !Sample_geo_accession = GSM3200748 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447875 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200749 !Sample_title = cy88_cd45pos_5_D05_S233_comb !Sample_geo_accession = GSM3200749 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447874 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200750 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_C05_S509_comb !Sample_geo_accession = GSM3200750 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447873 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200751 !Sample_title = cy94_cd45pos_4_H11_S95_comb !Sample_geo_accession = GSM3200751 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447872 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200752 !Sample_title = cy78_CD45_neg_2_A08_S584_comb !Sample_geo_accession = GSM3200752 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447871 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200753 !Sample_title = cy72_CD45_pos_C10_S898_comb !Sample_geo_accession = GSM3200753 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447870 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200754 !Sample_title = CY94CD45POS_1_B07_S115_comb !Sample_geo_accession = GSM3200754 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447869 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200755 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_C07_S319_comb !Sample_geo_accession = GSM3200755 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448523 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200756 !Sample_title = cy84_Primary_CD45_pos_D08_S428_comb !Sample_geo_accession = GSM3200756 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448522 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200757 !Sample_title = CY89A_CD45_POS_6_A04_S100_comb !Sample_geo_accession = GSM3200757 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448521 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200758 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_H07_S1051_comb !Sample_geo_accession = GSM3200758 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448520 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200759 !Sample_title = cy94_cd45neg_cd90pos_H02_S374_comb !Sample_geo_accession = GSM3200759 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448519 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200760 !Sample_title = CY88CD45_150813_F08_S356_comb !Sample_geo_accession = GSM3200760 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448518 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200761 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_C07_S511_comb !Sample_geo_accession = GSM3200761 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448517 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200762 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_C04_S1084_comb !Sample_geo_accession = GSM3200762 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448516 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200763 !Sample_title = CY88CD45POS_2_G07_S463_comb !Sample_geo_accession = GSM3200763 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448515 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200764 !Sample_title = CY84_PRIM_POS_ALL_8_A02_S98_comb !Sample_geo_accession = GSM3200764 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448514 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200765 !Sample_title = CY88_3_C09_S609_comb !Sample_geo_accession = GSM3200765 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448513 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200766 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_H09_S573_comb !Sample_geo_accession = GSM3200766 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448512 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200767 !Sample_title = cy82_CD45_pos_1_D05_S521_comb !Sample_geo_accession = GSM3200767 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448511 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200768 !Sample_title = cy94_cd45neg_cd90pos_B08_S308_comb !Sample_geo_accession = GSM3200768 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448510 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200769 !Sample_title = cy60_1_cd_45_pos_3_E06_S342_comb !Sample_geo_accession = GSM3200769 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448509 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200770 !Sample_title = cy78_CD45_neg_2_B07_S595_comb !Sample_geo_accession = GSM3200770 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel78 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448576 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200771 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_B12_S984_comb !Sample_geo_accession = GSM3200771 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448575 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200772 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_F10_S550_comb !Sample_geo_accession = GSM3200772 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448574 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200773 !Sample_title = cy82_CD45_pos_2_B04_S496_comb !Sample_geo_accession = GSM3200773 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448572 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200774 !Sample_title = Cy72_CD45_D08_S716_comb !Sample_geo_accession = GSM3200774 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448571 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200775 !Sample_title = cy94_cd45pos_A07_S103_comb !Sample_geo_accession = GSM3200775 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448570 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200776 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_E03_S1011_comb !Sample_geo_accession = GSM3200776 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448569 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200777 !Sample_title = cy88_cd45pos_5_G04_S268_comb !Sample_geo_accession = GSM3200777 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448568 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200778 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_G12_S564_comb !Sample_geo_accession = GSM3200778 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448567 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200779 !Sample_title = cy72_CD45_pos_C05_S893_comb !Sample_geo_accession = GSM3200779 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448566 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200780 !Sample_title = CY84_PRIM_POS_ALL_6_D12_S432_comb !Sample_geo_accession = GSM3200780 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448565 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200781 !Sample_title = CY89COREQ1_F06_S354_comb !Sample_geo_accession = GSM3200781 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448564 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200782 !Sample_title = Cy72_CD45_A04_S676_comb !Sample_geo_accession = GSM3200782 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448563 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200783 !Sample_title = CY89COREQ1_G10_S370_comb !Sample_geo_accession = GSM3200783 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448562 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200784 !Sample_title = cy72_CD45_pos_A02_S866_comb !Sample_geo_accession = GSM3200784 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448561 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200785 !Sample_title = cy79_p3_CD45_pos_PD1_neg_G11_S179_comb !Sample_geo_accession = GSM3200785 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448107 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200786 !Sample_title = cy94_cd45neg_cd90pos_G11_S371_comb !Sample_geo_accession = GSM3200786 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448106 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200787 !Sample_title = cy80_Cd45_pos_Pd1_neg_S293_E05_S293_comb !Sample_geo_accession = GSM3200787 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448105 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200788 !Sample_title = cy58_1_CD45_pos_C08_S608_comb !Sample_geo_accession = GSM3200788 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448104 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200789 !Sample_title = cy53_1_CD45_neg_G02_S362_comb !Sample_geo_accession = GSM3200789 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448103 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200790 !Sample_title = cy60_1_cd_45_pos_3_E07_S343_comb !Sample_geo_accession = GSM3200790 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448102 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200791 !Sample_title = CY84_PRIM_POS_ALL_6_E07_S439_comb !Sample_geo_accession = GSM3200791 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448101 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200792 !Sample_title = CY88CD45POS_7_E02_S242_comb !Sample_geo_accession = GSM3200792 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448164 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200793 !Sample_title = cy94_cd45pos_4_C03_S27_comb !Sample_geo_accession = GSM3200793 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448162 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200794 !Sample_title = cy60_1_cd_45_pos_3_A08_S296_comb !Sample_geo_accession = GSM3200794 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448161 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200795 !Sample_title = cy72_CD45_pos_F01_S925_comb !Sample_geo_accession = GSM3200795 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448160 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200796 !Sample_title = CY89COREQ1_H10_S382_comb !Sample_geo_accession = GSM3200796 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448159 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200797 !Sample_title = cy82_CD45_neg_2_E02_S242_comb !Sample_geo_accession = GSM3200797 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448158 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200798 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_B07_S499_comb !Sample_geo_accession = GSM3200798 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448157 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200799 !Sample_title = cy88_cd45pos_5_G03_S267_comb !Sample_geo_accession = GSM3200799 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448156 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200800 !Sample_title = cy94_cd45pos_4_C04_S28_comb !Sample_geo_accession = GSM3200800 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448154 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200801 !Sample_title = Cy80_II_CD45_A08_S872_comb !Sample_geo_accession = GSM3200801 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448153 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200802 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_E02_S530_comb !Sample_geo_accession = GSM3200802 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448152 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200803 !Sample_title = cy94_cd45neg_cd90pos_A12_S300_comb !Sample_geo_accession = GSM3200803 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448151 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200804 !Sample_title = cy60_1_cd_45_pos_4_E01_S49_comb !Sample_geo_accession = GSM3200804 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448150 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200805 !Sample_title = cy94_cd45pos_B12_S120_comb !Sample_geo_accession = GSM3200805 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448155 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200806 !Sample_title = cy84_Met_CD45_pos_H04_S952_comb !Sample_geo_accession = GSM3200806 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448149 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200807 !Sample_title = cy80_CD45_neg_F08_S452_comb !Sample_geo_accession = GSM3200807 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448148 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200808 !Sample_title = CY88CD45POS_7_C01_S217_comb !Sample_geo_accession = GSM3200808 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448147 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200809 !Sample_title = CY89FNAQ2_A11_S395_comb !Sample_geo_accession = GSM3200809 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448146 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200810 !Sample_title = cy58_1_CD45_pos_C01_S601_comb !Sample_geo_accession = GSM3200810 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448145 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200811 !Sample_title = CY89FNAQ2_C12_S420_comb !Sample_geo_accession = GSM3200811 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448144 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200812 !Sample_title = CY94CD45POS_1_F07_S163_comb !Sample_geo_accession = GSM3200812 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448143 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200813 !Sample_title = CY94CD45POS_1_F04_S160_comb !Sample_geo_accession = GSM3200813 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448142 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200814 !Sample_title = cy80_CD45_neg_F11_S455_comb !Sample_geo_accession = GSM3200814 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448141 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200815 !Sample_title = Cy71_CD45_G01_S553_comb !Sample_geo_accession = GSM3200815 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448689 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200816 !Sample_title = CY88CD45_150813_D07_S331_comb !Sample_geo_accession = GSM3200816 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448688 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200817 !Sample_title = CY88CD45POS_7_E03_S243_comb !Sample_geo_accession = GSM3200817 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448687 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200818 !Sample_title = cy79_p5_CD45_neg_PDL1_pos_G09_S753_comb !Sample_geo_accession = GSM3200818 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448686 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200819 !Sample_title = CY88CD45POS_2_G08_S464_comb !Sample_geo_accession = GSM3200819 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448685 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200820 !Sample_title = cy84_Met_CD45_pos_D06_S906_comb !Sample_geo_accession = GSM3200820 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448684 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200821 !Sample_title = cy88_cd45pos_5_B02_S206_comb !Sample_geo_accession = GSM3200821 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448683 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200822 !Sample_title = cy80_CD45_neg_B06_S882_comb !Sample_geo_accession = GSM3200822 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448682 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200823 !Sample_title = Cy72_CD45_F08_S740_comb !Sample_geo_accession = GSM3200823 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448681 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200824 !Sample_title = CY89FNAQ2_A06_S390_comb !Sample_geo_accession = GSM3200824 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448680 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200825 !Sample_title = cy88_cd_45_pos_3_F11_S647_comb !Sample_geo_accession = GSM3200825 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448679 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200826 !Sample_title = CY88CD45_150813_D05_S329_comb !Sample_geo_accession = GSM3200826 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448678 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200827 !Sample_title = cy94_cd45pos_4_H05_S89_comb !Sample_geo_accession = GSM3200827 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448677 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200828 !Sample_title = cy84_Primary_CD45_pos_B06_S402_comb !Sample_geo_accession = GSM3200828 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448676 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200829 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_C06_S1086_comb !Sample_geo_accession = GSM3200829 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448675 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200830 !Sample_title = cy94_cd45pos_B02_S110_comb !Sample_geo_accession = GSM3200830 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448674 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200831 !Sample_title = Cy74_CD45_H07_S475_comb !Sample_geo_accession = GSM3200831 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448140 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200832 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_F10_S262_comb !Sample_geo_accession = GSM3200832 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448139 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200833 !Sample_title = cy72_CD45_pos_A03_S867_comb !Sample_geo_accession = GSM3200833 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448138 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200834 !Sample_title = cy60_1_cd_45_pos_3_B03_S303_comb !Sample_geo_accession = GSM3200834 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448137 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200835 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_G04_S1036_comb !Sample_geo_accession = GSM3200835 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448193 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200836 !Sample_title = Cy72_CD45_D05_S713_comb !Sample_geo_accession = GSM3200836 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448192 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200837 !Sample_title = cy80_Cd45_pos_PD1_pos_S119_E11_S119_comb !Sample_geo_accession = GSM3200837 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448191 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200838 !Sample_title = CY88CD45POS_7_B11_S215_comb !Sample_geo_accession = GSM3200838 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448190 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200839 !Sample_title = CY88CD45_150813_F07_S355_comb !Sample_geo_accession = GSM3200839 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448189 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200840 !Sample_title = CY88_5_H02_S758_comb !Sample_geo_accession = GSM3200840 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448188 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200841 !Sample_title = CY94CD45POS_1_B12_S120_comb !Sample_geo_accession = GSM3200841 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448187 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200842 !Sample_title = cy84_Primary_CD45_pos_C11_S419_comb !Sample_geo_accession = GSM3200842 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448186 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200843 !Sample_title = Cy71_CD45_D02_S518_comb !Sample_geo_accession = GSM3200843 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel71 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448185 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200844 !Sample_title = cy94_cd45neg_cd90pos_C05_S317_comb !Sample_geo_accession = GSM3200844 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448184 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200845 !Sample_title = Cy72_CD45_F06_S738_comb !Sample_geo_accession = GSM3200845 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448183 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200846 !Sample_title = cy84_Primary_CD45_pos_H06_S474_comb !Sample_geo_accession = GSM3200846 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448182 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200847 !Sample_title = cy94_cd45pos_B01_S109_comb !Sample_geo_accession = GSM3200847 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448181 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200848 !Sample_title = cy94_cd45pos_4_D12_S48_comb !Sample_geo_accession = GSM3200848 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448180 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200849 !Sample_title = CY84_PRIM_POS_ALL_7_B04_S208_comb !Sample_geo_accession = GSM3200849 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448179 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200850 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_E07_S247_comb !Sample_geo_accession = GSM3200850 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448178 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200851 !Sample_title = cy60_1_cd_45_neg_3_H01_S565_comb !Sample_geo_accession = GSM3200851 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448177 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200852 !Sample_title = cy74_CD45_pos_A11_S683_comb !Sample_geo_accession = GSM3200852 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448176 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200853 !Sample_title = CY84_PRIM_POS_ALL_7_F10_S262_comb !Sample_geo_accession = GSM3200853 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448175 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200854 !Sample_title = cy94_cd45pos_F09_S165_comb !Sample_geo_accession = GSM3200854 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448174 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200855 !Sample_title = cy53_1_CD45_neg_D08_S332_comb !Sample_geo_accession = GSM3200855 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448173 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200856 !Sample_title = CY88CD45POS_2_G06_S462_comb !Sample_geo_accession = GSM3200856 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448172 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200857 !Sample_title = cy79_p3_CD45_neg_PD1_pos_AS_C3_R1_H12_S384_comb !Sample_geo_accession = GSM3200857 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448171 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200858 !Sample_title = CY89COREQ1_E03_S339_comb !Sample_geo_accession = GSM3200858 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448170 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200859 !Sample_title = cy74_CD45_pos_C12_S708_comb !Sample_geo_accession = GSM3200859 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448169 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200860 !Sample_title = cy79_p3_CD45_pos_PD1_neg_G07_S175_comb !Sample_geo_accession = GSM3200860 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448168 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200861 !Sample_title = cy80_Cd45_pos_Pd1_neg_S223_B07_S223_comb !Sample_geo_accession = GSM3200861 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448467 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200862 !Sample_title = Cy74_CD45_E10_S442_comb !Sample_geo_accession = GSM3200862 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448466 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200863 !Sample_title = cy94_cd45neg_cd90pos_C02_S314_comb !Sample_geo_accession = GSM3200863 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448465 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200864 !Sample_title = cy82_CD45_pos_3_C06_S30_comb !Sample_geo_accession = GSM3200864 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448464 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200865 !Sample_title = Cy81_Bulk_CD45_C12_S132_comb !Sample_geo_accession = GSM3200865 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448469 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200866 !Sample_title = Cy72_CD45_H04_S760_comb !Sample_geo_accession = GSM3200866 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448468 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200867 !Sample_title = cy60_1_cd_45_pos_4_B03_S15_comb !Sample_geo_accession = GSM3200867 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448463 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200868 !Sample_title = CY89A_CD45_POS_6_A08_S104_comb !Sample_geo_accession = GSM3200868 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448462 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200869 !Sample_title = Cy81_FNA_CD45_D05_S233_comb !Sample_geo_accession = GSM3200869 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448461 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200870 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_G01_S553_comb !Sample_geo_accession = GSM3200870 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448460 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200871 !Sample_title = cy79_p3_CD45_pos_PD1_neg_G04_S172_comb !Sample_geo_accession = GSM3200871 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448459 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200872 !Sample_title = cy94_cd45pos_4_B04_S16_comb !Sample_geo_accession = GSM3200872 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448458 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200873 !Sample_title = cy80_CD_90_pos_C02_S890_comb !Sample_geo_accession = GSM3200873 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448457 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200874 !Sample_title = CY89COREQ1_C08_S320_comb !Sample_geo_accession = GSM3200874 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448456 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200875 !Sample_title = cy60_1_cd_45_pos_4_D02_S38_comb !Sample_geo_accession = GSM3200875 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448455 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200876 !Sample_title = CY89A_CD45_POS_6_G01_S169_comb !Sample_geo_accession = GSM3200876 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448454 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200877 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_H06_S570_comb !Sample_geo_accession = GSM3200877 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448453 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200878 !Sample_title = CY88CD45POS_2_C01_S409_comb !Sample_geo_accession = GSM3200878 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448452 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200879 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_F04_S544_comb !Sample_geo_accession = GSM3200879 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448451 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200880 !Sample_title = CY89A_CD45_POS_6_B09_S117_comb !Sample_geo_accession = GSM3200880 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448450 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200881 !Sample_title = CY89A_Core_15_B10_S22_comb !Sample_geo_accession = GSM3200881 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448449 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200882 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_E11_S1019_comb !Sample_geo_accession = GSM3200882 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448448 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200883 !Sample_title = cy80_Cd45_pos_Pd1_neg_S219_B03_S219_comb !Sample_geo_accession = GSM3200883 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448447 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200884 !Sample_title = cy80_Cd45_pos_Pd1_neg_S227_B11_S227_comb !Sample_geo_accession = GSM3200884 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448446 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200885 !Sample_title = CY84_PRIM_POS_ALL_6_A01_S385_comb !Sample_geo_accession = GSM3200885 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448445 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200886 !Sample_title = cy60_1_cd_45_pos_4_G03_S75_comb !Sample_geo_accession = GSM3200886 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448444 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200887 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_G04_S556_comb !Sample_geo_accession = GSM3200887 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448443 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200888 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_F03_S1023_comb !Sample_geo_accession = GSM3200888 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448442 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200889 !Sample_title = CY84_PRIM_POS_ALL_7_E03_S243_comb !Sample_geo_accession = GSM3200889 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448441 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200890 !Sample_title = CY89A_Core_15_E04_S52_comb !Sample_geo_accession = GSM3200890 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448440 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200891 !Sample_title = cy79_p3_CD45_neg_PDL1_neg_A12_S204_comb !Sample_geo_accession = GSM3200891 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448480 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200892 !Sample_title = CY89A_Core_15_H11_S95_comb !Sample_geo_accession = GSM3200892 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448479 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200893 !Sample_title = cy79_p3_CD45_pos_PD1_pos_AS_C2_R1_F01_S445_comb !Sample_geo_accession = GSM3200893 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448478 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200894 !Sample_title = cy74_CD45_pos_B12_S696_comb !Sample_geo_accession = GSM3200894 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448477 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200895 !Sample_title = CY88_3_C02_S602_comb !Sample_geo_accession = GSM3200895 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448476 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200896 !Sample_title = cy72_CD45_pos_G07_S943_comb !Sample_geo_accession = GSM3200896 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448475 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200897 !Sample_title = CY88CD45POS_2_H12_S480_comb !Sample_geo_accession = GSM3200897 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448439 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200898 !Sample_title = cy79_p3_CD45_pos_PD1_neg_C05_S125_comb !Sample_geo_accession = GSM3200898 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448438 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200899 !Sample_title = cy88_cd_45_pos_3_F02_S638_comb !Sample_geo_accession = GSM3200899 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448508 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200900 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_B01_S493_comb !Sample_geo_accession = GSM3200900 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448507 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200901 !Sample_title = CY89FNA_F03_S255_comb !Sample_geo_accession = GSM3200901 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448506 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200902 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_F02_S1022_comb !Sample_geo_accession = GSM3200902 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448505 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200903 !Sample_title = cy60_1_cd_45_pos_4_B08_S20_comb !Sample_geo_accession = GSM3200903 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448504 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200904 !Sample_title = cy74_CD45_pos_B02_S686_comb !Sample_geo_accession = GSM3200904 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448503 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200905 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_D10_S1006_comb !Sample_geo_accession = GSM3200905 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448502 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200906 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_C10_S1090_comb !Sample_geo_accession = GSM3200906 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448501 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200907 !Sample_title = cy94_cd45pos_4_G02_S74_comb !Sample_geo_accession = GSM3200907 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448500 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200908 !Sample_title = cy84_Primary_CD45_pos_B02_S398_comb !Sample_geo_accession = GSM3200908 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448499 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200909 !Sample_title = cy53_1_CD45_pos_1_G08_S80_comb !Sample_geo_accession = GSM3200909 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448498 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200910 !Sample_title = CY58_1_CD45_D01_S997_comb !Sample_geo_accession = GSM3200910 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448497 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200911 !Sample_title = cy94_cd45pos_4_G04_S76_comb !Sample_geo_accession = GSM3200911 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448496 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200912 !Sample_title = cy60_1_cd_45_pos_3_G12_S372_comb !Sample_geo_accession = GSM3200912 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448495 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200913 !Sample_title = cy53_1_CD45_neg_G07_S367_comb !Sample_geo_accession = GSM3200913 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448494 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200914 !Sample_title = cy94_cd45neg_cd90pos_B07_S307_comb !Sample_geo_accession = GSM3200914 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448493 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200915 !Sample_title = cy84_Primary_CD45_pos_E07_S439_comb !Sample_geo_accession = GSM3200915 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448492 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200916 !Sample_title = CY94CD45POS_1_E04_S148_comb !Sample_geo_accession = GSM3200916 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448491 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200917 !Sample_title = cy79_p3_CD45_pos_PD1_neg_G05_S173_comb !Sample_geo_accession = GSM3200917 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448490 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200918 !Sample_title = Cy72_CD45_A08_S680_comb !Sample_geo_accession = GSM3200918 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448489 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200919 !Sample_title = cy80_Cd45_pos_Pd1_neg_S338_G02_S338_comb !Sample_geo_accession = GSM3200919 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448488 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200920 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_H05_S1049_comb !Sample_geo_accession = GSM3200920 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448487 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200921 !Sample_title = cy80_CD45_neg_C05_S893_comb !Sample_geo_accession = GSM3200921 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448486 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200922 !Sample_title = CY89A_Core_15_A03_S3_comb !Sample_geo_accession = GSM3200922 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448485 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200923 !Sample_title = CY94CD45POS_1_G09_S177_comb !Sample_geo_accession = GSM3200923 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448484 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200924 !Sample_title = CY89A_CD45_POS_6_D11_S143_comb !Sample_geo_accession = GSM3200924 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448483 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200925 !Sample_title = CY94CD45POS_1_E08_S152_comb !Sample_geo_accession = GSM3200925 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448482 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200926 !Sample_title = Cy80_II_CD45_H03_S951_comb !Sample_geo_accession = GSM3200926 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448481 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200927 !Sample_title = Cy72_CD45_H05_S761_comb !Sample_geo_accession = GSM3200927 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448067 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200928 !Sample_title = cy80_CD45_neg_D05_S905_comb !Sample_geo_accession = GSM3200928 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448136 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200929 !Sample_title = cy84_Primary_CD45_pos_G11_S467_comb !Sample_geo_accession = GSM3200929 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448135 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200930 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_E08_S1016_comb !Sample_geo_accession = GSM3200930 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448134 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200931 !Sample_title = cy80_Cd45_pos_PD1_pos_C08_S68_comb !Sample_geo_accession = GSM3200931 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448133 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200932 !Sample_title = cy60_1_cd_45_pos_3_F10_S358_comb !Sample_geo_accession = GSM3200932 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448132 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200933 !Sample_title = CY89A_CD45_POS_6_D07_S139_comb !Sample_geo_accession = GSM3200933 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448131 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200934 !Sample_title = cy80_Cd45_pos_Pd1_neg_S247_C07_S247_comb !Sample_geo_accession = GSM3200934 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448130 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200935 !Sample_title = cy80_CD45_pos_PD1_pos_G03_S171_comb !Sample_geo_accession = GSM3200935 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448129 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200936 !Sample_title = Cy72_CD45_G04_S748_comb !Sample_geo_accession = GSM3200936 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448128 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200937 !Sample_title = cy80_CD_90_pos_A02_S866_comb !Sample_geo_accession = GSM3200937 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448127 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200938 !Sample_title = CY88CD45POS_7_F04_S256_comb !Sample_geo_accession = GSM3200938 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448126 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200939 !Sample_title = cy88_cd45pos_5_E02_S242_comb !Sample_geo_accession = GSM3200939 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448125 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200940 !Sample_title = cy94_cd45pos_B09_S117_comb !Sample_geo_accession = GSM3200940 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448124 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200941 !Sample_title = cy88_cd_45_pos_3_H12_S672_comb !Sample_geo_accession = GSM3200941 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448123 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200942 !Sample_title = Cy81_Bulk_CD45_C08_S128_comb !Sample_geo_accession = GSM3200942 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448122 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200943 !Sample_title = cy60_1_cd_45_pos_4_H01_S85_comb !Sample_geo_accession = GSM3200943 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448121 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200944 !Sample_title = CY84_PRIM_POS_ALL_8_A01_S97_comb !Sample_geo_accession = GSM3200944 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448120 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200945 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_G12_S1044_comb !Sample_geo_accession = GSM3200945 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448119 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200946 !Sample_title = cy84_Met_CD45_pos_H05_S953_comb !Sample_geo_accession = GSM3200946 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448118 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200947 !Sample_title = CY94CD45POS_1_G12_S180_comb !Sample_geo_accession = GSM3200947 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448117 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200948 !Sample_title = CY88CD45POS_2_G01_S457_comb !Sample_geo_accession = GSM3200948 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448116 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200949 !Sample_title = cy53_1_CD45_pos_2_A10_S970_comb !Sample_geo_accession = GSM3200949 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448115 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200950 !Sample_title = CY89A_Core_15_A10_S10_comb !Sample_geo_accession = GSM3200950 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448112 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200951 !Sample_title = cy58_1_CD45_pos_F11_S647_comb !Sample_geo_accession = GSM3200951 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448111 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200952 !Sample_title = cy79_p4_CD45_neg_PDL1_neg_F05_S1121_comb !Sample_geo_accession = GSM3200952 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448110 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200953 !Sample_title = CY89A_Core_15_D02_S38_comb !Sample_geo_accession = GSM3200953 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448109 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200954 !Sample_title = cy79_p3_CD45_pos_PD1_neg_G12_S180_comb !Sample_geo_accession = GSM3200954 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448108 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200955 !Sample_title = CY88CD45POS_7_G04_S268_comb !Sample_geo_accession = GSM3200955 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448114 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200956 !Sample_title = Cy72_CD45_C08_S704_comb !Sample_geo_accession = GSM3200956 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448113 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200957 !Sample_title = CY88CD45POS_7_E09_S249_comb !Sample_geo_accession = GSM3200957 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447994 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200958 !Sample_title = CY88CD45POS_7_G03_S267_comb !Sample_geo_accession = GSM3200958 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447993 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200959 !Sample_title = cy94_cd45pos_A04_S100_comb !Sample_geo_accession = GSM3200959 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447992 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200960 !Sample_title = cy53_1_CD45_pos_2_E03_S1011_comb !Sample_geo_accession = GSM3200960 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447991 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200961 !Sample_title = cy80_cd_90_neg_H04_S184_comb !Sample_geo_accession = GSM3200961 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447990 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200962 !Sample_title = cy60_1_cd_45_pos_3_G06_S366_comb !Sample_geo_accession = GSM3200962 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447989 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200963 !Sample_title = CY88CD45POS_2_B07_S403_comb !Sample_geo_accession = GSM3200963 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447988 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200964 !Sample_title = CY88CD45_150813_D03_S327_comb !Sample_geo_accession = GSM3200964 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447987 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200965 !Sample_title = CY89A_Core_15_C11_S35_comb !Sample_geo_accession = GSM3200965 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447986 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200966 !Sample_title = CY94CD45POS_1_F06_S162_comb !Sample_geo_accession = GSM3200966 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447985 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200967 !Sample_title = CY88CD45_150813_E02_S338_comb !Sample_geo_accession = GSM3200967 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447984 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200968 !Sample_title = CY94CD45POS_1_H03_S183_comb !Sample_geo_accession = GSM3200968 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447983 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200969 !Sample_title = CY88CD45POS_7_C05_S221_comb !Sample_geo_accession = GSM3200969 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447982 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200970 !Sample_title = cy88_cd45pos_5_B05_S209_comb !Sample_geo_accession = GSM3200970 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447981 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200971 !Sample_title = CY89A_Core_15_F10_S70_comb !Sample_geo_accession = GSM3200971 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447980 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200972 !Sample_title = cy88_cd_45_pos_H05_S473_comb !Sample_geo_accession = GSM3200972 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447979 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200973 !Sample_title = CY84_PRIM_POS_ALL_8_E11_S155_comb !Sample_geo_accession = GSM3200973 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447978 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200974 !Sample_title = cy60_1_cd_45_pos_3_C04_S316_comb !Sample_geo_accession = GSM3200974 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447977 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200975 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_B06_S978_comb !Sample_geo_accession = GSM3200975 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447976 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200976 !Sample_title = cy60_1_cd_45_pos_3_H07_S379_comb !Sample_geo_accession = GSM3200976 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447975 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200977 !Sample_title = cy94_cd45pos_G09_S177_comb !Sample_geo_accession = GSM3200977 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447974 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200978 !Sample_title = cy82_CD45_neg_3_H02_S854_comb !Sample_geo_accession = GSM3200978 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel82 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447973 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200979 !Sample_title = CY84_PRIM_POS_ALL_7_E12_S252_comb !Sample_geo_accession = GSM3200979 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447972 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200980 !Sample_title = cy88_cd45pos_5_C02_S218_comb !Sample_geo_accession = GSM3200980 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447971 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200981 !Sample_title = CY89A_CD45_POS_6_B05_S113_comb !Sample_geo_accession = GSM3200981 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448032 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200982 !Sample_title = cy58_1_CD45_pos_E10_S634_comb !Sample_geo_accession = GSM3200982 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel58 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448031 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200983 !Sample_title = CY84_PRIM_POS_ALL_8_C07_S127_comb !Sample_geo_accession = GSM3200983 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448030 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200984 !Sample_title = cy60_1_cd_45_pos_3_E04_S340_comb !Sample_geo_accession = GSM3200984 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448059 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200985 !Sample_title = cy74_CD45_pos_A10_S682_comb !Sample_geo_accession = GSM3200985 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447970 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200986 !Sample_title = cy84_Primary_CD45_pos_C08_S416_comb !Sample_geo_accession = GSM3200986 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447969 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200987 !Sample_title = CY89COREQ1_G09_S369_comb !Sample_geo_accession = GSM3200987 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447968 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200988 !Sample_title = CY89CORE11_E02_S146_comb !Sample_geo_accession = GSM3200988 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448058 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200989 !Sample_title = CY89COREQ1_F08_S356_comb !Sample_geo_accession = GSM3200989 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448057 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200990 !Sample_title = cy94_cd45neg_cd90pos_H07_S379_comb !Sample_geo_accession = GSM3200990 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448056 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200991 !Sample_title = CY89A_Core_15_D07_S43_comb !Sample_geo_accession = GSM3200991 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448055 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200992 !Sample_title = CY89A_CD45_POS_6_G08_S176_comb !Sample_geo_accession = GSM3200992 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448054 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200993 !Sample_title = CY94CD45POS_1_D03_S135_comb !Sample_geo_accession = GSM3200993 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448053 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200994 !Sample_title = cy94_cd45pos_C09_S129_comb !Sample_geo_accession = GSM3200994 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448052 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200995 !Sample_title = CY84_PRIM_POS_ALL_7_A07_S199_comb !Sample_geo_accession = GSM3200995 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448051 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200996 !Sample_title = Cy81_Bulk_CD45_F10_S166_comb !Sample_geo_accession = GSM3200996 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel81 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448050 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200997 !Sample_title = cy94_cd45pos_4_H12_S96_comb !Sample_geo_accession = GSM3200997 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448049 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200998 !Sample_title = Cy72_CD45_H12_S864_comb !Sample_geo_accession = GSM3200998 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel72 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448048 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3200999 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_G07_S559_comb !Sample_geo_accession = GSM3200999 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448047 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201000 !Sample_title = Cy80_II_CD45_B04_S880_comb !Sample_geo_accession = GSM3201000 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448046 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201001 !Sample_title = cy88_cd45pos_5_D03_S231_comb !Sample_geo_accession = GSM3201001 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448045 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201002 !Sample_title = cy53_1_CD45_pos_2_H05_S1049_comb !Sample_geo_accession = GSM3201002 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448044 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201003 !Sample_title = CY84_PRIM_POS_ALL_7_D01_S229_comb !Sample_geo_accession = GSM3201003 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448043 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201004 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_B07_S979_comb !Sample_geo_accession = GSM3201004 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448042 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201005 !Sample_title = cy53_1_CD45_pos_1_B10_S22_comb !Sample_geo_accession = GSM3201005 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel53 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448041 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201006 !Sample_title = CY89A_CD45_POS_6_B04_S112_comb !Sample_geo_accession = GSM3201006 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448040 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201007 !Sample_title = cy94_cd45neg_cd90pos_A09_S297_comb !Sample_geo_accession = GSM3201007 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448039 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201008 !Sample_title = CY89A_CD45_POS_6_A09_S105_comb !Sample_geo_accession = GSM3201008 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448038 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201009 !Sample_title = cy80_CD45_pos_PD1_pos_D11_S143_comb !Sample_geo_accession = GSM3201009 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448037 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201010 !Sample_title = cy79_p1_CD45_neg_PDL1_neg_AS_C4_R1_D03_S135_comb !Sample_geo_accession = GSM3201010 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448036 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201011 !Sample_title = cy80_CD_90_pos_C07_S895_comb !Sample_geo_accession = GSM3201011 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448035 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201012 !Sample_title = cy80_CD_90_pos_H08_S956_comb !Sample_geo_accession = GSM3201012 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448034 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201013 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_H12_S576_comb !Sample_geo_accession = GSM3201013 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448099 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201014 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_F07_S1027_comb !Sample_geo_accession = GSM3201014 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448098 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201015 !Sample_title = cy79_p1_CD45_pos_PD1_neg_AS_C1_R2_F08_S548_comb !Sample_geo_accession = GSM3201015 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448033 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201016 !Sample_title = CY89COREQ1_C01_S313_comb !Sample_geo_accession = GSM3201016 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448100 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201017 !Sample_title = CY88CD45POS_2_F09_S453_comb !Sample_geo_accession = GSM3201017 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel88 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448599 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201018 !Sample_title = Cy74_CD45_G11_S467_comb !Sample_geo_accession = GSM3201018 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel74 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448598 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201019 !Sample_title = CY84_PRIM_POS_ALL_7_A01_S193_comb !Sample_geo_accession = GSM3201019 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel84 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448597 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201020 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_C03_S987_comb !Sample_geo_accession = GSM3201020 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448596 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201021 !Sample_title = cy60_1_cd_45_pos_HC_pos_HLDAR_pos_A04_S964_comb !Sample_geo_accession = GSM3201021 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448595 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201022 !Sample_title = CY89COREQ1_C07_S319_comb !Sample_geo_accession = GSM3201022 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel89 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448594 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201023 !Sample_title = cy79_p3_CD45_pos_PD1_neg_F05_S161_comb !Sample_geo_accession = GSM3201023 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel79 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448593 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201024 !Sample_title = cy60_1_cd_45_pos_4_A11_S11_comb !Sample_geo_accession = GSM3201024 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel60 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448592 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201025 !Sample_title = cy80_Cd45_pos_Pd1_neg_S296_E08_S296_comb !Sample_geo_accession = GSM3201025 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel80 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448591 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201026 !Sample_title = cy94_cd45pos_D01_S133_comb !Sample_geo_accession = GSM3201026 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel94 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448590 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201027 !Sample_title = merck_cd45pos_PL3_S292 !Sample_geo_accession = GSM3201027 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448589 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201028 !Sample_title = merck_cd45pos_PL3_S337 !Sample_geo_accession = GSM3201028 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448588 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201029 !Sample_title = merck_cd45negcd90neg_PL3_S247 !Sample_geo_accession = GSM3201029 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448587 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201030 !Sample_title = merck_cd45pos_PL3_S360 !Sample_geo_accession = GSM3201030 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448586 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201031 !Sample_title = merck_cd45pos_PL3_S365 !Sample_geo_accession = GSM3201031 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448585 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201032 !Sample_title = merck_cd45pos_PL3_S340 !Sample_geo_accession = GSM3201032 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448584 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201033 !Sample_title = merck_cd45negcd90neg_PL3_S270 !Sample_geo_accession = GSM3201033 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448583 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201034 !Sample_title = merck_cd45pos_PL3_S328 !Sample_geo_accession = GSM3201034 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448582 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201035 !Sample_title = merck_cd45negcd90neg_PL3_S268 !Sample_geo_accession = GSM3201035 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448581 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201036 !Sample_title = merck_cd45negcd90neg_PL3_S241 !Sample_geo_accession = GSM3201036 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448580 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201037 !Sample_title = merck_cd45negcd90neg_PL3_S195 !Sample_geo_accession = GSM3201037 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448579 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201038 !Sample_title = merck_cd45pos_PL3_S357 !Sample_geo_accession = GSM3201038 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448578 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201039 !Sample_title = merck_cd45negcd90neg_PL3_S223 !Sample_geo_accession = GSM3201039 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448577 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201040 !Sample_title = merck_cd45negcd90neg_PL3_S245 !Sample_geo_accession = GSM3201040 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448649 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201041 !Sample_title = merck_cd45pos_PL3_S336 !Sample_geo_accession = GSM3201041 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448648 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201042 !Sample_title = merck_cd45pos_PL3_S344 !Sample_geo_accession = GSM3201042 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448646 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201043 !Sample_title = merck_cd45pos_PL3_S324 !Sample_geo_accession = GSM3201043 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448645 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201044 !Sample_title = merck_cd45pos_PL3_S325 !Sample_geo_accession = GSM3201044 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448644 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201045 !Sample_title = merck_cd45pos_PL3_S347 !Sample_geo_accession = GSM3201045 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448643 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201046 !Sample_title = merck_cd45pos_PL3_S348 !Sample_geo_accession = GSM3201046 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448432 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201047 !Sample_title = merck_cd45pos_PL3_S352 !Sample_geo_accession = GSM3201047 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448647 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201048 !Sample_title = merck_cd45pos_PL3_S377 !Sample_geo_accession = GSM3201048 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448431 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201049 !Sample_title = merck_cd45negcd90neg_PL3_S227 !Sample_geo_accession = GSM3201049 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448430 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201050 !Sample_title = merck_cd45pos_PL3_S312 !Sample_geo_accession = GSM3201050 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448429 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201051 !Sample_title = merck_cd45pos_PL3_S299 !Sample_geo_accession = GSM3201051 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448428 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201052 !Sample_title = merck_cd45negcd90neg_PL3_S221 !Sample_geo_accession = GSM3201052 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448427 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201053 !Sample_title = merck_cd45negcd90neg_PL3_S275 !Sample_geo_accession = GSM3201053 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448426 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201054 !Sample_title = merck_cd45negcd90neg_PL3_S232 !Sample_geo_accession = GSM3201054 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448425 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201055 !Sample_title = merck_cd45pos_PL3_S333 !Sample_geo_accession = GSM3201055 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448424 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201056 !Sample_title = merck_cd45negcd90neg_PL3_S250 !Sample_geo_accession = GSM3201056 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448423 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201057 !Sample_title = merck_cd45negcd90neg_PL3_S278 !Sample_geo_accession = GSM3201057 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448422 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201058 !Sample_title = merck_cd45pos_PL3_S375 !Sample_geo_accession = GSM3201058 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448421 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201059 !Sample_title = merck_cd45pos_PL3_S327 !Sample_geo_accession = GSM3201059 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448420 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201060 !Sample_title = merck_cd45negcd90neg_PL3_S261 !Sample_geo_accession = GSM3201060 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448419 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201061 !Sample_title = merck_cd45negcd90neg_PL3_S238 !Sample_geo_accession = GSM3201061 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448418 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201062 !Sample_title = merck_cd45pos_PL3_S374 !Sample_geo_accession = GSM3201062 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448417 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201063 !Sample_title = merck_cd45pos_PL3_S351 !Sample_geo_accession = GSM3201063 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448416 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201064 !Sample_title = merck_cd45negcd90neg_PL3_S236 !Sample_geo_accession = GSM3201064 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448415 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201065 !Sample_title = merck_cd45negcd90neg_PL3_S226 !Sample_geo_accession = GSM3201065 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448414 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201066 !Sample_title = merck_cd45pos_PL3_S335 !Sample_geo_accession = GSM3201066 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448413 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201067 !Sample_title = merck_cd45pos_PL3_S326 !Sample_geo_accession = GSM3201067 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448412 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201068 !Sample_title = merck_cd45pos_PL3_S346 !Sample_geo_accession = GSM3201068 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448411 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201069 !Sample_title = merck_cd45negcd90neg_PL3_S231 !Sample_geo_accession = GSM3201069 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448410 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201070 !Sample_title = merck_cd45pos_PL3_S294 !Sample_geo_accession = GSM3201070 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448409 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201071 !Sample_title = merck_cd45negcd90neg_PL3_S197 !Sample_geo_accession = GSM3201071 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448408 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201072 !Sample_title = merck_cd45pos_PL3_S305 !Sample_geo_accession = GSM3201072 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448407 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201073 !Sample_title = merck_cd45negcd90neg_PL3_S277 !Sample_geo_accession = GSM3201073 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448406 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201074 !Sample_title = merck_cd45pos_PL3_S308 !Sample_geo_accession = GSM3201074 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448472 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201075 !Sample_title = merck_cd45pos_PL3_S378 !Sample_geo_accession = GSM3201075 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448471 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201076 !Sample_title = merck_cd45pos_PL3_S356 !Sample_geo_accession = GSM3201076 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448470 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201077 !Sample_title = merck_cd45negcd90neg_PL3_S202 !Sample_geo_accession = GSM3201077 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448434 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201078 !Sample_title = merck_cd45pos_PL3_S359 !Sample_geo_accession = GSM3201078 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448433 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201079 !Sample_title = merck_cd45pos_PL3_S316 !Sample_geo_accession = GSM3201079 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448312 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201080 !Sample_title = merck_cd45pos_PL3_S289 !Sample_geo_accession = GSM3201080 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448311 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201081 !Sample_title = merck_cd45pos_PL3_S368 !Sample_geo_accession = GSM3201081 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448310 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201082 !Sample_title = merck_cd45pos_PL3_S290 !Sample_geo_accession = GSM3201082 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448309 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201083 !Sample_title = merck_cd45pos_PL3_S293 !Sample_geo_accession = GSM3201083 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448308 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201084 !Sample_title = merck_cd45pos_PL3_S382 !Sample_geo_accession = GSM3201084 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448307 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201085 !Sample_title = merck_cd45pos_PL3_S311 !Sample_geo_accession = GSM3201085 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448306 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201086 !Sample_title = merck_cd45negcd90neg_PL3_S204 !Sample_geo_accession = GSM3201086 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448305 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201087 !Sample_title = merck_cd45negcd90neg_PL3_S213 !Sample_geo_accession = GSM3201087 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448304 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201088 !Sample_title = merck_cd45pos_PL3_S319 !Sample_geo_accession = GSM3201088 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448303 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201089 !Sample_title = merck_cd45pos_PL3_S315 !Sample_geo_accession = GSM3201089 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448302 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201090 !Sample_title = merck_cd45pos_PL3_S363 !Sample_geo_accession = GSM3201090 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448301 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201091 !Sample_title = merck_cd45negcd90neg_PL3_S217 !Sample_geo_accession = GSM3201091 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448300 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201092 !Sample_title = merck_cd45negcd90neg_PL3_S287 !Sample_geo_accession = GSM3201092 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448299 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201093 !Sample_title = merck_cd45pos_PL3_S361 !Sample_geo_accession = GSM3201093 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448298 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201094 !Sample_title = merck_cd45pos_PL3_S300 !Sample_geo_accession = GSM3201094 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448297 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201095 !Sample_title = merck_cd45negcd90neg_PL3_S219 !Sample_geo_accession = GSM3201095 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448296 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201096 !Sample_title = merck_cd45pos_PL3_S381 !Sample_geo_accession = GSM3201096 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448295 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201097 !Sample_title = merck_cd45negcd90neg_PL3_S243 !Sample_geo_accession = GSM3201097 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448365 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201098 !Sample_title = merck_cd45negcd90neg_PL3_S214 !Sample_geo_accession = GSM3201098 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448364 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201099 !Sample_title = merck_cd45pos_PL3_S349 !Sample_geo_accession = GSM3201099 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448363 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201100 !Sample_title = merck_cd45negcd90neg_PL3_S260 !Sample_geo_accession = GSM3201100 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448362 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201101 !Sample_title = merck_cd45pos_PL3_S323 !Sample_geo_accession = GSM3201101 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448361 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201102 !Sample_title = merck_cd45negcd90neg_PL3_S211 !Sample_geo_accession = GSM3201102 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448360 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201103 !Sample_title = merck_cd45pos_PL3_S362 !Sample_geo_accession = GSM3201103 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448356 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201104 !Sample_title = merck_cd45negcd90neg_PL3_S276 !Sample_geo_accession = GSM3201104 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448355 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201105 !Sample_title = merck_cd45negcd90neg_PL3_S205 !Sample_geo_accession = GSM3201105 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448354 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201106 !Sample_title = merck_cd45negcd90neg_PL3_S240 !Sample_geo_accession = GSM3201106 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448353 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201107 !Sample_title = merck_cd45pos_PL3_S383 !Sample_geo_accession = GSM3201107 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448359 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201108 !Sample_title = merck_cd45pos_PL3_S379 !Sample_geo_accession = GSM3201108 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448358 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201109 !Sample_title = merck_cd45negcd90neg_PL3_S248 !Sample_geo_accession = GSM3201109 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448357 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201110 !Sample_title = merck_cd45pos_PL3_S314 !Sample_geo_accession = GSM3201110 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448352 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201111 !Sample_title = merck_cd45negcd90neg_PL3_S209 !Sample_geo_accession = GSM3201111 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448351 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201112 !Sample_title = merck_cd45pos_PL3_S355 !Sample_geo_accession = GSM3201112 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448350 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201113 !Sample_title = merck_cd45negcd90neg_PL3_S252 !Sample_geo_accession = GSM3201113 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448349 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201114 !Sample_title = merck_cd45negcd90neg_PL3_S198 !Sample_geo_accession = GSM3201114 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448348 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201115 !Sample_title = merck_cd45negcd90neg_PL3_S196 !Sample_geo_accession = GSM3201115 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448313 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201116 !Sample_title = merck_cd45negcd90neg_PL3_S208 !Sample_geo_accession = GSM3201116 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448560 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201117 !Sample_title = merck_cd45pos_PL3_S329 !Sample_geo_accession = GSM3201117 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448559 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201118 !Sample_title = merck_cd45negcd90neg_PL3_S230 !Sample_geo_accession = GSM3201118 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448558 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201119 !Sample_title = merck_cd45negcd90neg_PL3_S274 !Sample_geo_accession = GSM3201119 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448557 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201120 !Sample_title = merck_cd45pos_PL3_S370 !Sample_geo_accession = GSM3201120 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448556 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201121 !Sample_title = merck_cd45negcd90neg_PL3_S271 !Sample_geo_accession = GSM3201121 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448555 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201122 !Sample_title = merck_cd45pos_PL3_S342 !Sample_geo_accession = GSM3201122 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448554 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201123 !Sample_title = merck_cd45pos_PL3_S291 !Sample_geo_accession = GSM3201123 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448553 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201124 !Sample_title = merck_cd45negcd90neg_PL3_S255 !Sample_geo_accession = GSM3201124 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448552 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201125 !Sample_title = merck_cd45pos_PL3_S366 !Sample_geo_accession = GSM3201125 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448551 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201126 !Sample_title = merck_cd45negcd90neg_PL3_S272 !Sample_geo_accession = GSM3201126 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448550 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201127 !Sample_title = merck_cd45negcd90neg_PL3_S256 !Sample_geo_accession = GSM3201127 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448549 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201128 !Sample_title = merck_cd45negcd90neg_PL3_S253 !Sample_geo_accession = GSM3201128 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448548 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201129 !Sample_title = merck_cd45negcd90neg_PL3_S235 !Sample_geo_accession = GSM3201129 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448547 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201130 !Sample_title = merck_cd45negcd90neg_PL3_S218 !Sample_geo_accession = GSM3201130 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448546 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201131 !Sample_title = merck_cd45pos_PL3_S341 !Sample_geo_accession = GSM3201131 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448545 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201132 !Sample_title = merck_cd45negcd90neg_PL3_S193 !Sample_geo_accession = GSM3201132 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448544 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201133 !Sample_title = merck_cd45pos_PL3_S350 !Sample_geo_accession = GSM3201133 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448612 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201134 !Sample_title = merck_cd45pos_PL3_S331 !Sample_geo_accession = GSM3201134 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448611 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201135 !Sample_title = merck_cd45pos_PL3_S358 !Sample_geo_accession = GSM3201135 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448606 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201136 !Sample_title = merck_cd45pos_PL3_S297 !Sample_geo_accession = GSM3201136 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448605 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201137 !Sample_title = merck_cd45negcd90neg_PL3_S207 !Sample_geo_accession = GSM3201137 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448610 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201138 !Sample_title = merck_cd45pos_PL3_S298 !Sample_geo_accession = GSM3201138 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448609 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201139 !Sample_title = merck_cd45pos_PL3_S364 !Sample_geo_accession = GSM3201139 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448608 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201140 !Sample_title = merck_cd45pos_PL3_S321 !Sample_geo_accession = GSM3201140 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448607 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201141 !Sample_title = merck_cd45pos_PL3_S313 !Sample_geo_accession = GSM3201141 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448604 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201142 !Sample_title = merck_cd45negcd90neg_PL3_S239 !Sample_geo_accession = GSM3201142 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448603 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201143 !Sample_title = merck_cd45negcd90neg_PL3_S242 !Sample_geo_accession = GSM3201143 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448602 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201144 !Sample_title = Merck_CD45pos_pl4_S55 !Sample_geo_accession = GSM3201144 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448601 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201145 !Sample_title = Merck_CD45pos_pl4_S62 !Sample_geo_accession = GSM3201145 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448600 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201146 !Sample_title = Merck_CD45pos_pl4_S28 !Sample_geo_accession = GSM3201146 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448347 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201147 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S108 !Sample_geo_accession = GSM3201147 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448346 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201148 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S164 !Sample_geo_accession = GSM3201148 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448345 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201149 !Sample_title = Merck_CD45pos_pl4_S81 !Sample_geo_accession = GSM3201149 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448344 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201150 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S131 !Sample_geo_accession = GSM3201150 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448343 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201151 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S168 !Sample_geo_accession = GSM3201151 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448342 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201152 !Sample_title = Merck_CD45pos_pl4_S51 !Sample_geo_accession = GSM3201152 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448341 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201153 !Sample_title = Merck_CD45pos_pl4_S14 !Sample_geo_accession = GSM3201153 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448340 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201154 !Sample_title = Merck_CD45pos_pl4_S68 !Sample_geo_accession = GSM3201154 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448339 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201155 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S127 !Sample_geo_accession = GSM3201155 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448338 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201156 !Sample_title = Merck_CD45pos_pl4_S39 !Sample_geo_accession = GSM3201156 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448337 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201157 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S166 !Sample_geo_accession = GSM3201157 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448336 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201158 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S137 !Sample_geo_accession = GSM3201158 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448335 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201159 !Sample_title = Merck_CD45pos_pl4_S5 !Sample_geo_accession = GSM3201159 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448334 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201160 !Sample_title = Merck_CD45pos_pl4_S31 !Sample_geo_accession = GSM3201160 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448333 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201161 !Sample_title = Merck_CD45pos_pl4_S37 !Sample_geo_accession = GSM3201161 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448332 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201162 !Sample_title = Merck_CD45pos_pl4_S58 !Sample_geo_accession = GSM3201162 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448331 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201163 !Sample_title = Merck_CD45pos_pl4_S1 !Sample_geo_accession = GSM3201163 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448405 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201164 !Sample_title = Merck_CD45pos_pl4_S9 !Sample_geo_accession = GSM3201164 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448404 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201165 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S157 !Sample_geo_accession = GSM3201165 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448403 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201166 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S175 !Sample_geo_accession = GSM3201166 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448402 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201167 !Sample_title = Merck_CD45pos_pl4_S63 !Sample_geo_accession = GSM3201167 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448401 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201168 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S124 !Sample_geo_accession = GSM3201168 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448400 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201169 !Sample_title = Merck_CD45pos_pl4_S33 !Sample_geo_accession = GSM3201169 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448399 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201170 !Sample_title = Merck_CD45pos_pl4_S2 !Sample_geo_accession = GSM3201170 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448398 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201171 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S107 !Sample_geo_accession = GSM3201171 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448397 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201172 !Sample_title = Merck_CD45pos_pl4_S80 !Sample_geo_accession = GSM3201172 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448396 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201173 !Sample_title = Merck_CD45pos_pl4_S17 !Sample_geo_accession = GSM3201173 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448395 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201174 !Sample_title = Merck_CD45pos_pl4_S38 !Sample_geo_accession = GSM3201174 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448394 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201175 !Sample_title = Merck_CD45pos_pl4_S89 !Sample_geo_accession = GSM3201175 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448393 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201176 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S177 !Sample_geo_accession = GSM3201176 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449059 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201177 !Sample_title = Merck_CD45pos_pl4_S56 !Sample_geo_accession = GSM3201177 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449058 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201178 !Sample_title = Merck_CD45pos_pl4_S21 !Sample_geo_accession = GSM3201178 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449057 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201179 !Sample_title = Merck_CD45pos_pl4_S48 !Sample_geo_accession = GSM3201179 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449056 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201180 !Sample_title = Merck_CD45pos_pl4_S84 !Sample_geo_accession = GSM3201180 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449055 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201181 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S171 !Sample_geo_accession = GSM3201181 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449054 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201182 !Sample_title = Merck_CD45pos_pl4_S30 !Sample_geo_accession = GSM3201182 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449053 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201183 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S133 !Sample_geo_accession = GSM3201183 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449052 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201184 !Sample_title = Merck_CD45pos_pl4_S75 !Sample_geo_accession = GSM3201184 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449051 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201185 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S185 !Sample_geo_accession = GSM3201185 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449050 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201186 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S128 !Sample_geo_accession = GSM3201186 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449049 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201187 !Sample_title = Merck_CD45pos_pl4_S50 !Sample_geo_accession = GSM3201187 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449048 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201188 !Sample_title = Merck_CD45pos_pl4_S95 !Sample_geo_accession = GSM3201188 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449047 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201189 !Sample_title = Merck_CD45pos_pl4_S44 !Sample_geo_accession = GSM3201189 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449046 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201190 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S140 !Sample_geo_accession = GSM3201190 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449045 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201191 !Sample_title = Merck_CD45pos_pl4_S65 !Sample_geo_accession = GSM3201191 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449044 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201192 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S145 !Sample_geo_accession = GSM3201192 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449043 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201193 !Sample_title = Merck_CD45pos_pl4_S77 !Sample_geo_accession = GSM3201193 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449042 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201194 !Sample_title = Merck_CD45pos_pl4_S22 !Sample_geo_accession = GSM3201194 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449041 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201195 !Sample_title = Merck_CD45pos_pl4_S83 !Sample_geo_accession = GSM3201195 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449040 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201196 !Sample_title = Merck_CD45pos_pl4_S32 !Sample_geo_accession = GSM3201196 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449039 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201197 !Sample_title = Merck_CD45pos_pl4_S34 !Sample_geo_accession = GSM3201197 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449038 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201198 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S123 !Sample_geo_accession = GSM3201198 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449037 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201199 !Sample_title = Merck_CD45pos_pl4_S46 !Sample_geo_accession = GSM3201199 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449036 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201200 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S190 !Sample_geo_accession = GSM3201200 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449035 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201201 !Sample_title = Merck_CD45pos_pl4_S18 !Sample_geo_accession = GSM3201201 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449034 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201202 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S97 !Sample_geo_accession = GSM3201202 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449033 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201203 !Sample_title = Merck_CD45pos_pl4_S54 !Sample_geo_accession = GSM3201203 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449105 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201204 !Sample_title = Merck_CD45pos_pl4_S24 !Sample_geo_accession = GSM3201204 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449104 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201205 !Sample_title = Merck_CD45pos_pl4_S90 !Sample_geo_accession = GSM3201205 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449103 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201206 !Sample_title = Merck_CD45pos_pl4_S13 !Sample_geo_accession = GSM3201206 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449959 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201207 !Sample_title = Merck_CD45pos_pl4_S85 !Sample_geo_accession = GSM3201207 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449958 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201208 !Sample_title = Merck_CD45pos_pl4_S26 !Sample_geo_accession = GSM3201208 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449957 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201209 !Sample_title = Merck_CD45pos_pl4_S43 !Sample_geo_accession = GSM3201209 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449956 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201210 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S176 !Sample_geo_accession = GSM3201210 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449955 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201211 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S188 !Sample_geo_accession = GSM3201211 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449954 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201212 !Sample_title = Merck_CD45pos_pl4_S78 !Sample_geo_accession = GSM3201212 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449953 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201213 !Sample_title = Merck_CD45pos_pl4_S73 !Sample_geo_accession = GSM3201213 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449952 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201214 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S138 !Sample_geo_accession = GSM3201214 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449951 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201215 !Sample_title = Merck_CD45pos_pl4_S74 !Sample_geo_accession = GSM3201215 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449950 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201216 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S170 !Sample_geo_accession = GSM3201216 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449949 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201217 !Sample_title = Merck_CD45pos_pl4_S41 !Sample_geo_accession = GSM3201217 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449948 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201218 !Sample_title = Merck_CD45pos_pl4_S49 !Sample_geo_accession = GSM3201218 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449947 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201219 !Sample_title = Merck_CD45pos_pl4_S8 !Sample_geo_accession = GSM3201219 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449946 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201220 !Sample_title = Merck_CD45pos_pl4_S94 !Sample_geo_accession = GSM3201220 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449945 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201221 !Sample_title = Merck_CD45pos_pl4_S93 !Sample_geo_accession = GSM3201221 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449944 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201222 !Sample_title = Merck_CD45pos_pl4_S57 !Sample_geo_accession = GSM3201222 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449943 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201223 !Sample_title = Merck_CD45pos_pl4_S67 !Sample_geo_accession = GSM3201223 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449942 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201224 !Sample_title = Merck_CD45pos_pl4_S29 !Sample_geo_accession = GSM3201224 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449941 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201225 !Sample_title = Merck_CD45pos_pl4_S11 !Sample_geo_accession = GSM3201225 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449940 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201226 !Sample_title = Merck_CD45pos_pl4_S71 !Sample_geo_accession = GSM3201226 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449939 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201227 !Sample_title = Merck_CD45pos_pl4_S66 !Sample_geo_accession = GSM3201227 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449938 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201228 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S132 !Sample_geo_accession = GSM3201228 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449937 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201229 !Sample_title = Merck_CD45pos_pl4_S12 !Sample_geo_accession = GSM3201229 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449936 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201230 !Sample_title = Merck_CD45pos_pl4_S25 !Sample_geo_accession = GSM3201230 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449935 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201231 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S119 !Sample_geo_accession = GSM3201231 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449934 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201232 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S162 !Sample_geo_accession = GSM3201232 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449933 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201233 !Sample_title = Merck_CD45pos_pl4_S15 !Sample_geo_accession = GSM3201233 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449932 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201234 !Sample_title = Merck_CD45pos_pl4_S76 !Sample_geo_accession = GSM3201234 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449931 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201235 !Sample_title = Merck_CD45pos_pl4_S23 !Sample_geo_accession = GSM3201235 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449930 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201236 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S142 !Sample_geo_accession = GSM3201236 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449777 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201237 !Sample_title = Merck_CD45pos_pl4_S53 !Sample_geo_accession = GSM3201237 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449776 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201238 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S187 !Sample_geo_accession = GSM3201238 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449775 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201239 !Sample_title = Merck_CD45pos_pl4_S3 !Sample_geo_accession = GSM3201239 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449774 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201240 !Sample_title = Merck_CD45pos_pl4_S10 !Sample_geo_accession = GSM3201240 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449773 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201241 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S143 !Sample_geo_accession = GSM3201241 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449772 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201242 !Sample_title = Merck_CD45pos_pl4_S70 !Sample_geo_accession = GSM3201242 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449771 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201243 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S172 !Sample_geo_accession = GSM3201243 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449770 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201244 !Sample_title = Merck_CD45pos_pl4_S61 !Sample_geo_accession = GSM3201244 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449769 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201245 !Sample_title = Merck_CD45pos_pl4_S4 !Sample_geo_accession = GSM3201245 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449768 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201246 !Sample_title = Merck_CD45pos_pl4_S82 !Sample_geo_accession = GSM3201246 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449767 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201247 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S181 !Sample_geo_accession = GSM3201247 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449766 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201248 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S101 !Sample_geo_accession = GSM3201248 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449892 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201249 !Sample_title = Merck_CD45pos_pl4_S35 !Sample_geo_accession = GSM3201249 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449891 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201250 !Sample_title = Merck_CD45pos_pl4_S79 !Sample_geo_accession = GSM3201250 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449890 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201251 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S103 !Sample_geo_accession = GSM3201251 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449889 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201252 !Sample_title = Merck_CD45pos_pl4_S59 !Sample_geo_accession = GSM3201252 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449888 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201253 !Sample_title = Merck_CD45pos_pl4_S42 !Sample_geo_accession = GSM3201253 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449887 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201254 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S149 !Sample_geo_accession = GSM3201254 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449886 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201255 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S139 !Sample_geo_accession = GSM3201255 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449820 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201256 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S165 !Sample_geo_accession = GSM3201256 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449819 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201257 !Sample_title = Merck_CD45pos_pl4_S91 !Sample_geo_accession = GSM3201257 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449885 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201258 !Sample_title = Merck_CD45pos_pl4_S64 !Sample_geo_accession = GSM3201258 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449884 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201259 !Sample_title = Merck_CD45pos_pl4_S52 !Sample_geo_accession = GSM3201259 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449823 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201260 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S184 !Sample_geo_accession = GSM3201260 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449822 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201261 !Sample_title = Merck_CD45pos_pl4_S16 !Sample_geo_accession = GSM3201261 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449821 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201262 !Sample_title = Merck_CD45pos_pl4_S20 !Sample_geo_accession = GSM3201262 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449817 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201263 !Sample_title = Merck_CD45pos_pl4_S86 !Sample_geo_accession = GSM3201263 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449816 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201264 !Sample_title = Merck_CD45pos_pl4_S72 !Sample_geo_accession = GSM3201264 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449815 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201265 !Sample_title = Merck_CD45pos_pl4_S47 !Sample_geo_accession = GSM3201265 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09449814 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201266 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S106 !Sample_geo_accession = GSM3201266 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448672 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201267 !Sample_title = Merck_CD45pos_pl4_S60 !Sample_geo_accession = GSM3201267 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448671 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201268 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S158 !Sample_geo_accession = GSM3201268 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448670 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201269 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S144 !Sample_geo_accession = GSM3201269 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448669 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201270 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S121 !Sample_geo_accession = GSM3201270 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448668 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201271 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S173 !Sample_geo_accession = GSM3201271 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448667 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201272 !Sample_title = Merck_CD45pos_pl4_S88 !Sample_geo_accession = GSM3201272 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448666 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201273 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S178 !Sample_geo_accession = GSM3201273 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448665 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201274 !Sample_title = Merck_CD45pos_pl4_S6 !Sample_geo_accession = GSM3201274 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448664 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201275 !Sample_title = Merck_CD45neg_EPCAMpos_pl4_S161 !Sample_geo_accession = GSM3201275 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel194 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448663 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201276 !Sample_title = cy102_CD45pos_S131 !Sample_geo_accession = GSM3201276 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448662 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201277 !Sample_title = cy112_CD45pos_S322 !Sample_geo_accession = GSM3201277 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448661 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201278 !Sample_title = cy98_CD45pos_CD8pos_S87 !Sample_geo_accession = GSM3201278 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448660 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201279 !Sample_title = cy110_CD45pos_S275 !Sample_geo_accession = GSM3201279 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448659 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201280 !Sample_title = cy110_CD45pos_S208 !Sample_geo_accession = GSM3201280 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448658 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201281 !Sample_title = cy98_CD45pos_CD8pos_S8 !Sample_geo_accession = GSM3201281 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448657 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201282 !Sample_title = cy110_CD45pos_S264 !Sample_geo_accession = GSM3201282 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448656 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201283 !Sample_title = cy102_CD45pos_S101 !Sample_geo_accession = GSM3201283 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448655 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201284 !Sample_title = cy110_CD45pos_S278 !Sample_geo_accession = GSM3201284 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448654 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201285 !Sample_title = cy98_CD45pos_CD8pos_S4 !Sample_geo_accession = GSM3201285 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448653 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201286 !Sample_title = cy98_CD45pos_CD8pos_S77 !Sample_geo_accession = GSM3201286 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448652 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201287 !Sample_title = cy110_CD45pos_S267 !Sample_geo_accession = GSM3201287 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448651 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201288 !Sample_title = cy98_CD45pos_CD8pos_S90 !Sample_geo_accession = GSM3201288 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448650 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201289 !Sample_title = cy98_CD45pos_CD8pos_S62 !Sample_geo_accession = GSM3201289 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448709 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201290 !Sample_title = cy102_CD45pos_S166 !Sample_geo_accession = GSM3201290 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448708 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201291 !Sample_title = cy110_CD45pos_S226 !Sample_geo_accession = GSM3201291 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448707 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201292 !Sample_title = cy112_CD45pos_S333 !Sample_geo_accession = GSM3201292 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448706 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201293 !Sample_title = cy102_CD45pos_S117 !Sample_geo_accession = GSM3201293 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448705 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201294 !Sample_title = cy102_CD45pos_S148 !Sample_geo_accession = GSM3201294 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448704 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201295 !Sample_title = cy98_CD45pos_CD8pos_S34 !Sample_geo_accession = GSM3201295 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448703 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201296 !Sample_title = cy102_CD45pos_S178 !Sample_geo_accession = GSM3201296 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448673 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201297 !Sample_title = cy98_CD45pos_CD8pos_S26 !Sample_geo_accession = GSM3201297 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448744 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201298 !Sample_title = cy110_CD45pos_S241 !Sample_geo_accession = GSM3201298 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448743 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201299 !Sample_title = cy110_CD45pos_S204 !Sample_geo_accession = GSM3201299 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448742 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201300 !Sample_title = cy110_CD45pos_S282 !Sample_geo_accession = GSM3201300 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448741 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201301 !Sample_title = cy98_CD45pos_CD8pos_S54 !Sample_geo_accession = GSM3201301 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448740 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201302 !Sample_title = cy102_CD45pos_S108 !Sample_geo_accession = GSM3201302 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448739 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201303 !Sample_title = cy102_CD45pos_S105 !Sample_geo_accession = GSM3201303 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448738 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201304 !Sample_title = cy110_CD45pos_S256 !Sample_geo_accession = GSM3201304 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448737 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201305 !Sample_title = cy98_CD45pos_CD8pos_S21 !Sample_geo_accession = GSM3201305 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448736 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201306 !Sample_title = cy112_CD45pos_S346 !Sample_geo_accession = GSM3201306 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448735 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201307 !Sample_title = cy110_CD45pos_S230 !Sample_geo_accession = GSM3201307 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448734 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201308 !Sample_title = cy110_CD45pos_S266 !Sample_geo_accession = GSM3201308 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448733 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201309 !Sample_title = cy112_CD45pos_S317 !Sample_geo_accession = GSM3201309 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448732 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201310 !Sample_title = cy102_CD45pos_S159 !Sample_geo_accession = GSM3201310 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448731 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201311 !Sample_title = cy110_CD45pos_S227 !Sample_geo_accession = GSM3201311 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448730 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201312 !Sample_title = cy98_CD45pos_CD8pos_S67 !Sample_geo_accession = GSM3201312 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448729 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201313 !Sample_title = cy110_CD45pos_S195 !Sample_geo_accession = GSM3201313 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448728 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201314 !Sample_title = cy98_CD45pos_CD8pos_S18 !Sample_geo_accession = GSM3201314 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448727 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201315 !Sample_title = cy98_CD45pos_CD8pos_S52 !Sample_geo_accession = GSM3201315 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448726 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201316 !Sample_title = cy98_CD45pos_CD8pos_S43 !Sample_geo_accession = GSM3201316 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448725 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201317 !Sample_title = cy98_CD45pos_CD8pos_S83 !Sample_geo_accession = GSM3201317 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448724 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201318 !Sample_title = cy102_CD45pos_S164 !Sample_geo_accession = GSM3201318 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448722 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201319 !Sample_title = cy112_CD45pos_S375 !Sample_geo_accession = GSM3201319 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448721 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201320 !Sample_title = cy110_CD45pos_S283 !Sample_geo_accession = GSM3201320 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448720 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201321 !Sample_title = cy110_CD45pos_S239 !Sample_geo_accession = GSM3201321 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448719 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201322 !Sample_title = cy110_CD45pos_S221 !Sample_geo_accession = GSM3201322 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448718 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201323 !Sample_title = cy98_CD45pos_CD8pos_S82 !Sample_geo_accession = GSM3201323 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448717 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201324 !Sample_title = cy98_CD45pos_CD8pos_S76 !Sample_geo_accession = GSM3201324 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448716 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201325 !Sample_title = cy98_CD45pos_CD8pos_S55 !Sample_geo_accession = GSM3201325 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448715 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201326 !Sample_title = cy112_CD45pos_S299 !Sample_geo_accession = GSM3201326 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448847 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201327 !Sample_title = cy112_CD45pos_S327 !Sample_geo_accession = GSM3201327 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448846 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201328 !Sample_title = cy98_CD45pos_CD8pos_S2 !Sample_geo_accession = GSM3201328 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448845 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201329 !Sample_title = cy110_CD45pos_S271 !Sample_geo_accession = GSM3201329 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448844 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201330 !Sample_title = cy112_CD45pos_S324 !Sample_geo_accession = GSM3201330 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448843 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201331 !Sample_title = cy98_CD45pos_CD8pos_S31 !Sample_geo_accession = GSM3201331 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448842 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201332 !Sample_title = cy112_CD45pos_S381 !Sample_geo_accession = GSM3201332 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448841 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201333 !Sample_title = cy98_CD45pos_CD8pos_S36 !Sample_geo_accession = GSM3201333 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448840 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201334 !Sample_title = cy102_CD45pos_S98 !Sample_geo_accession = GSM3201334 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448839 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201335 !Sample_title = cy102_CD45pos_S147 !Sample_geo_accession = GSM3201335 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448838 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201336 !Sample_title = cy98_CD45pos_CD8pos_S91 !Sample_geo_accession = GSM3201336 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448837 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201337 !Sample_title = cy102_CD45pos_S123 !Sample_geo_accession = GSM3201337 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448836 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201338 !Sample_title = cy98_CD45pos_CD8pos_S63 !Sample_geo_accession = GSM3201338 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448835 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201339 !Sample_title = cy98_CD45pos_CD8pos_S17 !Sample_geo_accession = GSM3201339 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448834 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201340 !Sample_title = cy102_CD45pos_S158 !Sample_geo_accession = GSM3201340 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448833 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201341 !Sample_title = cy102_CD45pos_S184 !Sample_geo_accession = GSM3201341 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448832 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201342 !Sample_title = cy98_CD45pos_CD8pos_S59 !Sample_geo_accession = GSM3201342 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448830 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201343 !Sample_title = cy110_CD45pos_S252 !Sample_geo_accession = GSM3201343 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448829 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201344 !Sample_title = cy98_CD45pos_CD8pos_S48 !Sample_geo_accession = GSM3201344 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448828 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201345 !Sample_title = cy110_CD45pos_S262 !Sample_geo_accession = GSM3201345 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448827 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201346 !Sample_title = cy102_CD45pos_S103 !Sample_geo_accession = GSM3201346 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448826 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201347 !Sample_title = cy102_CD45pos_S124 !Sample_geo_accession = GSM3201347 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448831 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201348 !Sample_title = cy98_CD45pos_CD8pos_S15 !Sample_geo_accession = GSM3201348 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448825 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201349 !Sample_title = cy110_CD45pos_S218 !Sample_geo_accession = GSM3201349 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448824 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201350 !Sample_title = cy102_CD45pos_S130 !Sample_geo_accession = GSM3201350 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448823 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201351 !Sample_title = cy98_CD45pos_CD8pos_S37 !Sample_geo_accession = GSM3201351 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448822 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201352 !Sample_title = cy98_CD45pos_CD8pos_S69 !Sample_geo_accession = GSM3201352 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448821 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201353 !Sample_title = cy98_CD45pos_CD8pos_S94 !Sample_geo_accession = GSM3201353 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448820 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201354 !Sample_title = cy102_CD45pos_S106 !Sample_geo_accession = GSM3201354 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448819 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201355 !Sample_title = cy110_CD45pos_S231 !Sample_geo_accession = GSM3201355 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448818 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201356 !Sample_title = cy98_CD45pos_CD8pos_S47 !Sample_geo_accession = GSM3201356 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448702 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201357 !Sample_title = cy110_CD45pos_S197 !Sample_geo_accession = GSM3201357 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448701 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201358 !Sample_title = cy112_CD45pos_S355 !Sample_geo_accession = GSM3201358 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448700 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201359 !Sample_title = cy102_CD45pos_S171 !Sample_geo_accession = GSM3201359 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448699 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201360 !Sample_title = cy98_CD45pos_CD8pos_S27 !Sample_geo_accession = GSM3201360 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448698 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201361 !Sample_title = cy98_CD45pos_CD8pos_S6 !Sample_geo_accession = GSM3201361 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448697 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201362 !Sample_title = cy110_CD45pos_S240 !Sample_geo_accession = GSM3201362 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448696 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201363 !Sample_title = cy102_CD45pos_S188 !Sample_geo_accession = GSM3201363 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448695 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201364 !Sample_title = cy110_CD45pos_S285 !Sample_geo_accession = GSM3201364 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448694 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201365 !Sample_title = cy102_CD45pos_S141 !Sample_geo_accession = GSM3201365 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448693 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201366 !Sample_title = cy102_CD45pos_S169 !Sample_geo_accession = GSM3201366 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448692 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201367 !Sample_title = cy98_CD45pos_CD8pos_S12 !Sample_geo_accession = GSM3201367 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448691 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201368 !Sample_title = cy102_CD45pos_S153 !Sample_geo_accession = GSM3201368 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448690 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201369 !Sample_title = cy102_CD45pos_S129 !Sample_geo_accession = GSM3201369 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450151 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201370 !Sample_title = cy98_CD45pos_CD8pos_S33 !Sample_geo_accession = GSM3201370 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450150 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201371 !Sample_title = cy110_CD45pos_S254 !Sample_geo_accession = GSM3201371 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450149 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201372 !Sample_title = cy110_CD45pos_S205 !Sample_geo_accession = GSM3201372 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450148 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201373 !Sample_title = cy98_CD45pos_CD8pos_S65 !Sample_geo_accession = GSM3201373 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450147 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201374 !Sample_title = cy98_CD45pos_CD8pos_S7 !Sample_geo_accession = GSM3201374 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450146 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201375 !Sample_title = cy102_CD45pos_S177 !Sample_geo_accession = GSM3201375 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450209 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201376 !Sample_title = cy102_CD45pos_S187 !Sample_geo_accession = GSM3201376 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450208 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201377 !Sample_title = cy110_CD45pos_S251 !Sample_geo_accession = GSM3201377 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450207 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201378 !Sample_title = cy110_CD45pos_S214 !Sample_geo_accession = GSM3201378 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450206 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201379 !Sample_title = cy102_CD45pos_S99 !Sample_geo_accession = GSM3201379 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450205 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201380 !Sample_title = cy110_CD45pos_S242 !Sample_geo_accession = GSM3201380 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450204 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201381 !Sample_title = cy110_CD45pos_S238 !Sample_geo_accession = GSM3201381 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450203 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201382 !Sample_title = cy110_CD45pos_S202 !Sample_geo_accession = GSM3201382 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450202 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201383 !Sample_title = cy110_CD45pos_S259 !Sample_geo_accession = GSM3201383 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450201 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201384 !Sample_title = cy112_CD45pos_S344 !Sample_geo_accession = GSM3201384 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450200 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201385 !Sample_title = cy110_CD45pos_S250 !Sample_geo_accession = GSM3201385 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450199 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201386 !Sample_title = cy98_CD45pos_CD8pos_S22 !Sample_geo_accession = GSM3201386 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450198 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201387 !Sample_title = cy98_CD45pos_CD8pos_S58 !Sample_geo_accession = GSM3201387 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450197 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201388 !Sample_title = cy112_CD45pos_S306 !Sample_geo_accession = GSM3201388 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450196 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201389 !Sample_title = cy98_CD45pos_CD8pos_S95 !Sample_geo_accession = GSM3201389 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450195 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201390 !Sample_title = cy102_CD45pos_S112 !Sample_geo_accession = GSM3201390 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450194 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201391 !Sample_title = cy102_CD45pos_S114 !Sample_geo_accession = GSM3201391 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450193 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201392 !Sample_title = cy102_CD45pos_S97 !Sample_geo_accession = GSM3201392 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450192 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201393 !Sample_title = cy98_CD45pos_CD8pos_S73 !Sample_geo_accession = GSM3201393 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450191 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201394 !Sample_title = cy98_CD45pos_CD8pos_S53 !Sample_geo_accession = GSM3201394 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450190 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201395 !Sample_title = cy110_CD45pos_S220 !Sample_geo_accession = GSM3201395 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450189 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201396 !Sample_title = cy102_CD45pos_S125 !Sample_geo_accession = GSM3201396 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450188 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201397 !Sample_title = cy102_CD45pos_S190 !Sample_geo_accession = GSM3201397 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450187 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201398 !Sample_title = cy112_CD45pos_S359 !Sample_geo_accession = GSM3201398 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450142 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201399 !Sample_title = cy102_CD45pos_S120 !Sample_geo_accession = GSM3201399 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450141 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201400 !Sample_title = cy110_CD45pos_S199 !Sample_geo_accession = GSM3201400 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450140 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201401 !Sample_title = cy102_CD45pos_S132 !Sample_geo_accession = GSM3201401 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450139 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201402 !Sample_title = cy102_CD45pos_S121 !Sample_geo_accession = GSM3201402 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450138 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201403 !Sample_title = cy102_CD45pos_S176 !Sample_geo_accession = GSM3201403 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450137 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201404 !Sample_title = cy98_CD45pos_CD8pos_S66 !Sample_geo_accession = GSM3201404 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450136 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201405 !Sample_title = cy98_CD45pos_CD8pos_S11 !Sample_geo_accession = GSM3201405 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450135 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201406 !Sample_title = cy110_CD45pos_S219 !Sample_geo_accession = GSM3201406 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450129 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201407 !Sample_title = cy110_CD45pos_S228 !Sample_geo_accession = GSM3201407 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450134 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201408 !Sample_title = cy102_CD45pos_S155 !Sample_geo_accession = GSM3201408 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450133 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201409 !Sample_title = cy112_CD45pos_S330 !Sample_geo_accession = GSM3201409 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450132 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201410 !Sample_title = cy102_CD45pos_S126 !Sample_geo_accession = GSM3201410 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450131 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201411 !Sample_title = cy98_CD45pos_CD8pos_S30 !Sample_geo_accession = GSM3201411 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450130 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201412 !Sample_title = cy110_CD45pos_S248 !Sample_geo_accession = GSM3201412 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450128 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201413 !Sample_title = cy98_CD45pos_CD8pos_S39 !Sample_geo_accession = GSM3201413 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450127 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201414 !Sample_title = cy98_CD45pos_CD8pos_S56 !Sample_geo_accession = GSM3201414 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450126 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201415 !Sample_title = cy102_CD45pos_S151 !Sample_geo_accession = GSM3201415 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450125 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201416 !Sample_title = cy110_CD45pos_S253 !Sample_geo_accession = GSM3201416 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450124 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201417 !Sample_title = cy112_CD45pos_S289 !Sample_geo_accession = GSM3201417 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450123 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201418 !Sample_title = cy110_CD45pos_S225 !Sample_geo_accession = GSM3201418 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450122 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201419 !Sample_title = cy98_CD45pos_CD8pos_S25 !Sample_geo_accession = GSM3201419 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450121 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201420 !Sample_title = cy98_CD45pos_CD8pos_S24 !Sample_geo_accession = GSM3201420 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450120 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201421 !Sample_title = cy102_CD45pos_S175 !Sample_geo_accession = GSM3201421 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450119 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201422 !Sample_title = cy98_CD45pos_CD8pos_S51 !Sample_geo_accession = GSM3201422 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450118 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201423 !Sample_title = cy110_CD45pos_S246 !Sample_geo_accession = GSM3201423 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450117 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201424 !Sample_title = cy112_CD45pos_S295 !Sample_geo_accession = GSM3201424 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450116 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201425 !Sample_title = cy102_CD45pos_S167 !Sample_geo_accession = GSM3201425 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450115 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201426 !Sample_title = cy102_CD45pos_S181 !Sample_geo_accession = GSM3201426 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450181 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201427 !Sample_title = cy102_CD45pos_S165 !Sample_geo_accession = GSM3201427 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450180 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201428 !Sample_title = cy98_CD45pos_CD8pos_S29 !Sample_geo_accession = GSM3201428 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450179 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201429 !Sample_title = cy102_CD45pos_S185 !Sample_geo_accession = GSM3201429 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450178 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201430 !Sample_title = cy112_CD45pos_S345 !Sample_geo_accession = GSM3201430 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450177 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201431 !Sample_title = cy110_CD45pos_S233 !Sample_geo_accession = GSM3201431 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450176 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201432 !Sample_title = cy112_CD45pos_S297 !Sample_geo_accession = GSM3201432 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450175 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201433 !Sample_title = cy98_CD45pos_CD8pos_S79 !Sample_geo_accession = GSM3201433 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450172 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201434 !Sample_title = cy102_CD45pos_S146 !Sample_geo_accession = GSM3201434 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450171 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201435 !Sample_title = cy110_CD45pos_S263 !Sample_geo_accession = GSM3201435 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450170 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201436 !Sample_title = cy110_CD45pos_S274 !Sample_geo_accession = GSM3201436 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450169 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201437 !Sample_title = cy98_CD45pos_CD8pos_S5 !Sample_geo_accession = GSM3201437 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450174 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201438 !Sample_title = cy110_CD45pos_S234 !Sample_geo_accession = GSM3201438 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450173 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201439 !Sample_title = cy110_CD45pos_S247 !Sample_geo_accession = GSM3201439 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450168 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201440 !Sample_title = cy102_CD45pos_S186 !Sample_geo_accession = GSM3201440 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450167 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201441 !Sample_title = cy110_CD45pos_S277 !Sample_geo_accession = GSM3201441 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450166 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201442 !Sample_title = cy98_CD45pos_CD8pos_S35 !Sample_geo_accession = GSM3201442 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450165 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201443 !Sample_title = cy98_CD45pos_CD8pos_S92 !Sample_geo_accession = GSM3201443 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450164 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201444 !Sample_title = cy98_CD45pos_CD8pos_S57 !Sample_geo_accession = GSM3201444 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450163 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201445 !Sample_title = cy102_CD45pos_S149 !Sample_geo_accession = GSM3201445 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450162 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201446 !Sample_title = cy102_CD45pos_S128 !Sample_geo_accession = GSM3201446 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450161 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201447 !Sample_title = cy102_CD45pos_S113 !Sample_geo_accession = GSM3201447 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450103 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201448 !Sample_title = cy98_CD45pos_CD8pos_S40 !Sample_geo_accession = GSM3201448 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450102 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201449 !Sample_title = cy110_CD45pos_S212 !Sample_geo_accession = GSM3201449 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450101 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201450 !Sample_title = cy112_CD45pos_S341 !Sample_geo_accession = GSM3201450 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450100 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201451 !Sample_title = cy102_CD45pos_S170 !Sample_geo_accession = GSM3201451 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450099 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201452 !Sample_title = cy98_CD45pos_CD8pos_S89 !Sample_geo_accession = GSM3201452 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450098 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201453 !Sample_title = cy112_CD45pos_S351 !Sample_geo_accession = GSM3201453 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450097 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201454 !Sample_title = cy98_CD45pos_CD8pos_S80 !Sample_geo_accession = GSM3201454 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450096 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201455 !Sample_title = cy110_CD45pos_S287 !Sample_geo_accession = GSM3201455 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450095 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201456 !Sample_title = cy98_CD45pos_CD8pos_S74 !Sample_geo_accession = GSM3201456 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450094 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201457 !Sample_title = cy98_CD45pos_CD8pos_S38 !Sample_geo_accession = GSM3201457 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450093 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201458 !Sample_title = cy112_CD45pos_S293 !Sample_geo_accession = GSM3201458 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450092 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201459 !Sample_title = cy112_CD45pos_S291 !Sample_geo_accession = GSM3201459 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450091 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201460 !Sample_title = cy102_CD45pos_S183 !Sample_geo_accession = GSM3201460 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450090 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201461 !Sample_title = cy102_CD45pos_S162 !Sample_geo_accession = GSM3201461 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450089 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201462 !Sample_title = cy110_CD45pos_S279 !Sample_geo_accession = GSM3201462 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450088 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201463 !Sample_title = cy110_CD45pos_S245 !Sample_geo_accession = GSM3201463 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450087 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201464 !Sample_title = cy98_CD45pos_CD8pos_S3 !Sample_geo_accession = GSM3201464 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450086 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201465 !Sample_title = cy110_CD45pos_S232 !Sample_geo_accession = GSM3201465 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450085 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201466 !Sample_title = cy98_CD45pos_CD8pos_S68 !Sample_geo_accession = GSM3201466 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450084 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201467 !Sample_title = cy102_CD45pos_S102 !Sample_geo_accession = GSM3201467 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450083 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201468 !Sample_title = cy98_CD45pos_CD8pos_S49 !Sample_geo_accession = GSM3201468 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450082 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201469 !Sample_title = cy112_CD45pos_S357 !Sample_geo_accession = GSM3201469 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450081 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201470 !Sample_title = cy98_CD45pos_CD8pos_S20 !Sample_geo_accession = GSM3201470 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450080 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201471 !Sample_title = cy112_CD45pos_S382 !Sample_geo_accession = GSM3201471 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450079 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201472 !Sample_title = cy112_CD45pos_S348 !Sample_geo_accession = GSM3201472 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450078 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201473 !Sample_title = cy102_CD45pos_S160 !Sample_geo_accession = GSM3201473 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450077 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201474 !Sample_title = cy112_CD45pos_S379 !Sample_geo_accession = GSM3201474 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450145 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201475 !Sample_title = cy98_CD45pos_CD8pos_S84 !Sample_geo_accession = GSM3201475 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450144 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201476 !Sample_title = cy102_CD45pos_S189 !Sample_geo_accession = GSM3201476 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450143 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201477 !Sample_title = cy110_CD45pos_S269 !Sample_geo_accession = GSM3201477 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450215 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201478 !Sample_title = cy98_CD45pos_CD8pos_S1 !Sample_geo_accession = GSM3201478 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450214 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201479 !Sample_title = cy102_CD45pos_S127 !Sample_geo_accession = GSM3201479 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450213 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201480 !Sample_title = cy110_CD45pos_S213 !Sample_geo_accession = GSM3201480 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450212 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201481 !Sample_title = cy112_CD45pos_S328 !Sample_geo_accession = GSM3201481 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450211 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201482 !Sample_title = cy98_CD45pos_CD8pos_S10 !Sample_geo_accession = GSM3201482 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450210 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201483 !Sample_title = cy102_CD45pos_S104 !Sample_geo_accession = GSM3201483 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450280 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201484 !Sample_title = cy112_CD45pos_S335 !Sample_geo_accession = GSM3201484 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450279 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201485 !Sample_title = cy98_CD45pos_CD8pos_S46 !Sample_geo_accession = GSM3201485 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450278 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201486 !Sample_title = cy112_CD45pos_S378 !Sample_geo_accession = GSM3201486 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450277 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201487 !Sample_title = cy98_CD45pos_CD8pos_S93 !Sample_geo_accession = GSM3201487 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450276 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201488 !Sample_title = cy110_CD45pos_S244 !Sample_geo_accession = GSM3201488 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450275 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201489 !Sample_title = cy98_CD45pos_CD8pos_S45 !Sample_geo_accession = GSM3201489 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450274 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201490 !Sample_title = cy110_CD45pos_S276 !Sample_geo_accession = GSM3201490 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450273 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201491 !Sample_title = cy102_CD45pos_S163 !Sample_geo_accession = GSM3201491 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450272 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201492 !Sample_title = cy98_CD45pos_CD8pos_S70 !Sample_geo_accession = GSM3201492 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450271 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201493 !Sample_title = cy102_CD45pos_S152 !Sample_geo_accession = GSM3201493 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450268 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201494 !Sample_title = cy110_CD45pos_S260 !Sample_geo_accession = GSM3201494 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450266 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201495 !Sample_title = cy110_CD45pos_S224 !Sample_geo_accession = GSM3201495 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450265 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201496 !Sample_title = cy110_CD45pos_S216 !Sample_geo_accession = GSM3201496 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450264 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201497 !Sample_title = cy102_CD45pos_S122 !Sample_geo_accession = GSM3201497 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450270 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201498 !Sample_title = cy112_CD45pos_S315 !Sample_geo_accession = GSM3201498 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450269 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201499 !Sample_title = cy98_CD45pos_CD8pos_S41 !Sample_geo_accession = GSM3201499 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450263 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201500 !Sample_title = cy112_CD45pos_S347 !Sample_geo_accession = GSM3201500 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450262 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201501 !Sample_title = cy98_CD45pos_CD8pos_S61 !Sample_geo_accession = GSM3201501 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450261 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201502 !Sample_title = cy102_CD45pos_S180 !Sample_geo_accession = GSM3201502 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450260 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201503 !Sample_title = cy102_CD45pos_S145 !Sample_geo_accession = GSM3201503 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450259 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201504 !Sample_title = cy112_CD45pos_S339 !Sample_geo_accession = GSM3201504 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450258 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201505 !Sample_title = cy98_CD45pos_CD8pos_S71 !Sample_geo_accession = GSM3201505 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450257 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201506 !Sample_title = cy98_CD45pos_CD8pos_S42 !Sample_geo_accession = GSM3201506 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450256 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201507 !Sample_title = cy110_CD45pos_S209 !Sample_geo_accession = GSM3201507 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448392 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201508 !Sample_title = cy102_CD45pos_S116 !Sample_geo_accession = GSM3201508 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448391 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201509 !Sample_title = cy110_CD45pos_S243 !Sample_geo_accession = GSM3201509 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448390 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201510 !Sample_title = cy102_CD45pos_S168 !Sample_geo_accession = GSM3201510 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448389 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201511 !Sample_title = cy102_CD45pos_S154 !Sample_geo_accession = GSM3201511 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448388 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201512 !Sample_title = cy98_CD45pos_CD8pos_S78 !Sample_geo_accession = GSM3201512 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448387 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201513 !Sample_title = cy103_CD45pos_S158 !Sample_geo_accession = GSM3201513 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448386 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201514 !Sample_title = cy103_CD45pos_S175 !Sample_geo_accession = GSM3201514 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448385 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201515 !Sample_title = cy103_CD45pos_S169 !Sample_geo_accession = GSM3201515 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448384 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201516 !Sample_title = cy75_1CORE_CD45pos_S56 !Sample_geo_accession = GSM3201516 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448383 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201517 !Sample_title = cy75_1CORE_CD45pos_S76 !Sample_geo_accession = GSM3201517 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448382 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201518 !Sample_title = cy105_CD45pos_S285 !Sample_geo_accession = GSM3201518 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448381 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201519 !Sample_title = cy106_CD45pos_S289 !Sample_geo_accession = GSM3201519 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448380 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201520 !Sample_title = cy103_CD45pos_S107 !Sample_geo_accession = GSM3201520 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448379 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201521 !Sample_title = cy75_1CORE_CD45pos_S81 !Sample_geo_accession = GSM3201521 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448378 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201522 !Sample_title = cy75_1CORE_CD45pos_S25 !Sample_geo_accession = GSM3201522 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448377 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201523 !Sample_title = cy103_CD45pos_S113 !Sample_geo_accession = GSM3201523 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448376 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201524 !Sample_title = cy75_1CORE_CD45pos_S35 !Sample_geo_accession = GSM3201524 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448375 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201525 !Sample_title = cy105_CD45pos_S272 !Sample_geo_accession = GSM3201525 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448374 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201526 !Sample_title = cy75_1CORE_CD45pos_S88 !Sample_geo_accession = GSM3201526 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448373 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201527 !Sample_title = cy106_CD45pos_S318 !Sample_geo_accession = GSM3201527 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448372 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201528 !Sample_title = cy106_CD45pos_S301 !Sample_geo_accession = GSM3201528 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448371 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201529 !Sample_title = cy106_CD45pos_S365 !Sample_geo_accession = GSM3201529 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448370 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201530 !Sample_title = cy105_CD45pos_S283 !Sample_geo_accession = GSM3201530 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448369 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201531 !Sample_title = cy75_1CORE_CD45pos_S17 !Sample_geo_accession = GSM3201531 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448368 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201532 !Sample_title = cy103_CD45pos_S187 !Sample_geo_accession = GSM3201532 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448367 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201533 !Sample_title = cy75_1CORE_CD45pos_S23 !Sample_geo_accession = GSM3201533 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448366 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201534 !Sample_title = cy75_1CORE_CD45pos_S16 !Sample_geo_accession = GSM3201534 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448437 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201535 !Sample_title = cy103_CD45pos_S138 !Sample_geo_accession = GSM3201535 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448436 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201536 !Sample_title = cy103_CD45pos_S99 !Sample_geo_accession = GSM3201536 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448435 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201537 !Sample_title = cy75_1CORE_CD45pos_S33 !Sample_geo_accession = GSM3201537 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447590 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201538 !Sample_title = cy105_CD45pos_S215 !Sample_geo_accession = GSM3201538 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447589 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201539 !Sample_title = cy75_1CORE_CD45pos_S41 !Sample_geo_accession = GSM3201539 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447588 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201540 !Sample_title = cy103_CD45pos_S116 !Sample_geo_accession = GSM3201540 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447587 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201541 !Sample_title = cy106_CD45pos_S296 !Sample_geo_accession = GSM3201541 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447586 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201542 !Sample_title = cy75_1CORE_CD45pos_S95 !Sample_geo_accession = GSM3201542 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447585 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201543 !Sample_title = cy75_1CORE_CD45pos_S50 !Sample_geo_accession = GSM3201543 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447584 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201544 !Sample_title = cy75_1CORE_CD45pos_S38 !Sample_geo_accession = GSM3201544 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447583 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201545 !Sample_title = cy75_1CORE_CD45pos_S24 !Sample_geo_accession = GSM3201545 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447582 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201546 !Sample_title = cy105_CD45pos_S212 !Sample_geo_accession = GSM3201546 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447581 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201547 !Sample_title = cy105_CD45pos_S235 !Sample_geo_accession = GSM3201547 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447580 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201548 !Sample_title = cy75_1CORE_CD45pos_S52 !Sample_geo_accession = GSM3201548 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447579 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201549 !Sample_title = cy103_CD45pos_S118 !Sample_geo_accession = GSM3201549 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447578 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201550 !Sample_title = cy105_CD45pos_S267 !Sample_geo_accession = GSM3201550 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447577 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201551 !Sample_title = cy103_CD45pos_S188 !Sample_geo_accession = GSM3201551 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447576 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201552 !Sample_title = cy105_CD45pos_S196 !Sample_geo_accession = GSM3201552 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447575 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201553 !Sample_title = cy105_CD45pos_S204 !Sample_geo_accession = GSM3201553 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447574 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201554 !Sample_title = cy103_CD45pos_S106 !Sample_geo_accession = GSM3201554 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447573 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201555 !Sample_title = cy103_CD45pos_S152 !Sample_geo_accession = GSM3201555 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447572 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201556 !Sample_title = cy75_1CORE_CD45pos_S42 !Sample_geo_accession = GSM3201556 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447571 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201557 !Sample_title = cy105_CD45pos_S200 !Sample_geo_accession = GSM3201557 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447570 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201558 !Sample_title = cy106_CD45pos_S333 !Sample_geo_accession = GSM3201558 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447569 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201559 !Sample_title = cy75_1CORE_CD45pos_S64 !Sample_geo_accession = GSM3201559 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447568 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201560 !Sample_title = cy105_CD45pos_S252 !Sample_geo_accession = GSM3201560 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447567 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201561 !Sample_title = cy105_CD45pos_S206 !Sample_geo_accession = GSM3201561 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447566 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201562 !Sample_title = cy106_CD45pos_S309 !Sample_geo_accession = GSM3201562 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447565 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201563 !Sample_title = cy105_CD45pos_S234 !Sample_geo_accession = GSM3201563 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447564 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201564 !Sample_title = cy105_CD45pos_S263 !Sample_geo_accession = GSM3201564 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447563 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201565 !Sample_title = cy103_CD45pos_S105 !Sample_geo_accession = GSM3201565 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447637 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201566 !Sample_title = cy106_CD45pos_S371 !Sample_geo_accession = GSM3201566 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447636 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201567 !Sample_title = cy103_CD45pos_S159 !Sample_geo_accession = GSM3201567 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447635 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201568 !Sample_title = cy106_CD45pos_S381 !Sample_geo_accession = GSM3201568 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447634 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201569 !Sample_title = cy105_CD45pos_S226 !Sample_geo_accession = GSM3201569 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447633 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201570 !Sample_title = cy106_CD45pos_S291 !Sample_geo_accession = GSM3201570 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447632 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201571 !Sample_title = cy75_1CORE_CD45pos_S18 !Sample_geo_accession = GSM3201571 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447631 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201572 !Sample_title = cy103_CD45pos_S150 !Sample_geo_accession = GSM3201572 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447630 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201573 !Sample_title = cy105_CD45pos_S273 !Sample_geo_accession = GSM3201573 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447629 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201574 !Sample_title = cy105_CD45pos_S284 !Sample_geo_accession = GSM3201574 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447628 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201575 !Sample_title = cy105_CD45pos_S256 !Sample_geo_accession = GSM3201575 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447627 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201576 !Sample_title = cy75_1CORE_CD45pos_S2 !Sample_geo_accession = GSM3201576 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447626 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201577 !Sample_title = cy103_CD45pos_S122 !Sample_geo_accession = GSM3201577 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447625 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201578 !Sample_title = cy75_1CORE_CD45pos_S45 !Sample_geo_accession = GSM3201578 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447624 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201579 !Sample_title = cy75_1CORE_CD45pos_S74 !Sample_geo_accession = GSM3201579 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447623 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201580 !Sample_title = cy75_1CORE_CD45pos_S93 !Sample_geo_accession = GSM3201580 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447622 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201581 !Sample_title = cy105_CD45pos_S255 !Sample_geo_accession = GSM3201581 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447621 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201582 !Sample_title = cy103_CD45pos_S156 !Sample_geo_accession = GSM3201582 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447620 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201583 !Sample_title = cy105_CD45pos_S277 !Sample_geo_accession = GSM3201583 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447619 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201584 !Sample_title = cy75_1CORE_CD45pos_S75 !Sample_geo_accession = GSM3201584 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447618 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201585 !Sample_title = cy105_CD45pos_S209 !Sample_geo_accession = GSM3201585 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447617 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201586 !Sample_title = cy105_CD45pos_S254 !Sample_geo_accession = GSM3201586 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447616 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201587 !Sample_title = cy103_CD45pos_S139 !Sample_geo_accession = GSM3201587 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447615 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201588 !Sample_title = cy105_CD45pos_S268 !Sample_geo_accession = GSM3201588 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447614 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201589 !Sample_title = cy106_CD45pos_S335 !Sample_geo_accession = GSM3201589 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447613 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201590 !Sample_title = cy103_CD45pos_S149 !Sample_geo_accession = GSM3201590 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447612 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201591 !Sample_title = cy105_CD45pos_S214 !Sample_geo_accession = GSM3201591 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447611 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201592 !Sample_title = cy75_1CORE_CD45pos_S49 !Sample_geo_accession = GSM3201592 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447610 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201593 !Sample_title = cy105_CD45pos_S199 !Sample_geo_accession = GSM3201593 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447609 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201594 !Sample_title = cy105_CD45pos_S261 !Sample_geo_accession = GSM3201594 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447608 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201595 !Sample_title = cy75_1CORE_CD45pos_S58 !Sample_geo_accession = GSM3201595 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447607 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201596 !Sample_title = cy103_CD45pos_S173 !Sample_geo_accession = GSM3201596 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447606 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201597 !Sample_title = cy105_CD45pos_S207 !Sample_geo_accession = GSM3201597 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447605 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201598 !Sample_title = cy105_CD45pos_S220 !Sample_geo_accession = GSM3201598 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447604 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201599 !Sample_title = cy103_CD45pos_S142 !Sample_geo_accession = GSM3201599 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447603 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201600 !Sample_title = cy75_1CORE_CD45pos_S87 !Sample_geo_accession = GSM3201600 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447601 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201601 !Sample_title = cy105_CD45pos_S224 !Sample_geo_accession = GSM3201601 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447600 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201602 !Sample_title = cy75_1CORE_CD45pos_S63 !Sample_geo_accession = GSM3201602 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447673 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201603 !Sample_title = cy75_1CORE_CD45pos_S73 !Sample_geo_accession = GSM3201603 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447672 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201604 !Sample_title = cy103_CD45pos_S179 !Sample_geo_accession = GSM3201604 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447671 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201605 !Sample_title = cy103_CD45pos_S144 !Sample_geo_accession = GSM3201605 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447670 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201606 !Sample_title = cy105_CD45pos_S194 !Sample_geo_accession = GSM3201606 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447669 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201607 !Sample_title = cy103_CD45pos_S115 !Sample_geo_accession = GSM3201607 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447668 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201608 !Sample_title = cy106_CD45pos_S317 !Sample_geo_accession = GSM3201608 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447667 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201609 !Sample_title = cy103_CD45pos_S170 !Sample_geo_accession = GSM3201609 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447666 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201610 !Sample_title = cy75_1CORE_CD45pos_S14 !Sample_geo_accession = GSM3201610 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447665 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201611 !Sample_title = cy106_CD45pos_S358 !Sample_geo_accession = GSM3201611 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447664 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201612 !Sample_title = cy105_CD45pos_S244 !Sample_geo_accession = GSM3201612 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447663 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201613 !Sample_title = cy103_CD45pos_S167 !Sample_geo_accession = GSM3201613 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447662 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201614 !Sample_title = cy103_CD45pos_S133 !Sample_geo_accession = GSM3201614 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447661 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201615 !Sample_title = cy106_CD45pos_S352 !Sample_geo_accession = GSM3201615 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447660 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201616 !Sample_title = cy75_1CORE_CD45pos_S91 !Sample_geo_accession = GSM3201616 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447659 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201617 !Sample_title = cy75_1CORE_CD45pos_S85 !Sample_geo_accession = GSM3201617 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447658 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201618 !Sample_title = cy75_1CORE_CD45pos_S60 !Sample_geo_accession = GSM3201618 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447657 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201619 !Sample_title = cy105_CD45pos_S258 !Sample_geo_accession = GSM3201619 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447656 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201620 !Sample_title = cy103_CD45pos_S153 !Sample_geo_accession = GSM3201620 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447655 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201621 !Sample_title = cy103_CD45pos_S172 !Sample_geo_accession = GSM3201621 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447654 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201622 !Sample_title = cy75_1CORE_CD45pos_S68 !Sample_geo_accession = GSM3201622 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447653 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201623 !Sample_title = cy105_CD45pos_S228 !Sample_geo_accession = GSM3201623 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447652 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201624 !Sample_title = cy105_CD45pos_S286 !Sample_geo_accession = GSM3201624 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447651 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201625 !Sample_title = cy75_1CORE_CD45pos_S78 !Sample_geo_accession = GSM3201625 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447650 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201626 !Sample_title = cy105_CD45pos_S278 !Sample_geo_accession = GSM3201626 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447649 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201627 !Sample_title = cy75_1CORE_CD45pos_S13 !Sample_geo_accession = GSM3201627 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447648 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201628 !Sample_title = cy103_CD45pos_S190 !Sample_geo_accession = GSM3201628 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447647 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201629 !Sample_title = cy75_1CORE_CD45pos_S40 !Sample_geo_accession = GSM3201629 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447646 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201630 !Sample_title = cy105_CD45pos_S225 !Sample_geo_accession = GSM3201630 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447645 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201631 !Sample_title = cy103_CD45pos_S108 !Sample_geo_accession = GSM3201631 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447644 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201632 !Sample_title = cy75_1CORE_CD45pos_S70 !Sample_geo_accession = GSM3201632 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447643 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201633 !Sample_title = cy103_CD45pos_S128 !Sample_geo_accession = GSM3201633 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447642 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201634 !Sample_title = cy75_1CORE_CD45pos_S66 !Sample_geo_accession = GSM3201634 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447641 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201635 !Sample_title = cy103_CD45pos_S184 !Sample_geo_accession = GSM3201635 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447640 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201636 !Sample_title = cy103_CD45pos_S176 !Sample_geo_accession = GSM3201636 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447639 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201637 !Sample_title = cy105_CD45pos_S245 !Sample_geo_accession = GSM3201637 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447638 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201638 !Sample_title = cy105_CD45pos_S262 !Sample_geo_accession = GSM3201638 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447714 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201639 !Sample_title = cy106_CD45pos_S357 !Sample_geo_accession = GSM3201639 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447713 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201640 !Sample_title = cy103_CD45pos_S140 !Sample_geo_accession = GSM3201640 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447712 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201641 !Sample_title = cy103_CD45pos_S182 !Sample_geo_accession = GSM3201641 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447711 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201642 !Sample_title = cy105_CD45pos_S193 !Sample_geo_accession = GSM3201642 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447710 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201643 !Sample_title = cy105_CD45pos_S231 !Sample_geo_accession = GSM3201643 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447709 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201644 !Sample_title = cy75_1CORE_CD45pos_S44 !Sample_geo_accession = GSM3201644 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447708 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201645 !Sample_title = cy103_CD45pos_S186 !Sample_geo_accession = GSM3201645 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447707 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201646 !Sample_title = cy106_CD45pos_S355 !Sample_geo_accession = GSM3201646 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447706 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201647 !Sample_title = cy105_CD45pos_S270 !Sample_geo_accession = GSM3201647 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447705 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201648 !Sample_title = cy103_CD45pos_S110 !Sample_geo_accession = GSM3201648 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447704 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201649 !Sample_title = cy75_1CORE_CD45pos_S6 !Sample_geo_accession = GSM3201649 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447703 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201650 !Sample_title = cy75_1CORE_CD45pos_S71 !Sample_geo_accession = GSM3201650 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447702 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201651 !Sample_title = cy106_CD45pos_S379 !Sample_geo_accession = GSM3201651 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447701 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201652 !Sample_title = cy75_1CORE_CD45pos_S47 !Sample_geo_accession = GSM3201652 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447700 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201653 !Sample_title = cy105_CD45pos_S265 !Sample_geo_accession = GSM3201653 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447699 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201654 !Sample_title = cy75_1CORE_CD45pos_S34 !Sample_geo_accession = GSM3201654 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447698 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201655 !Sample_title = cy103_CD45pos_S145 !Sample_geo_accession = GSM3201655 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447697 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201656 !Sample_title = cy103_CD45pos_S155 !Sample_geo_accession = GSM3201656 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447696 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201657 !Sample_title = cy75_1CORE_CD45pos_S62 !Sample_geo_accession = GSM3201657 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447695 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201658 !Sample_title = cy105_CD45pos_S218 !Sample_geo_accession = GSM3201658 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447694 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201659 !Sample_title = cy75_1CORE_CD45pos_S48 !Sample_geo_accession = GSM3201659 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447693 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201660 !Sample_title = cy105_CD45pos_S242 !Sample_geo_accession = GSM3201660 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447692 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201661 !Sample_title = cy75_1CORE_CD45pos_S61 !Sample_geo_accession = GSM3201661 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447691 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201662 !Sample_title = cy103_CD45pos_S119 !Sample_geo_accession = GSM3201662 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447690 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201663 !Sample_title = cy103_CD45pos_S123 !Sample_geo_accession = GSM3201663 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447689 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201664 !Sample_title = cy103_CD45pos_S146 !Sample_geo_accession = GSM3201664 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447688 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201665 !Sample_title = cy103_CD45pos_S129 !Sample_geo_accession = GSM3201665 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447687 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201666 !Sample_title = cy105_CD45pos_S241 !Sample_geo_accession = GSM3201666 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447686 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201667 !Sample_title = cy75_1CORE_CD45pos_S7 !Sample_geo_accession = GSM3201667 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447685 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201668 !Sample_title = cy105_CD45pos_S246 !Sample_geo_accession = GSM3201668 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447684 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201669 !Sample_title = cy105_CD45pos_S247 !Sample_geo_accession = GSM3201669 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447683 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201670 !Sample_title = cy103_CD45pos_S151 !Sample_geo_accession = GSM3201670 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447682 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201671 !Sample_title = cy105_CD45pos_S275 !Sample_geo_accession = GSM3201671 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447681 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201672 !Sample_title = cy103_CD45pos_S147 !Sample_geo_accession = GSM3201672 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447680 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201673 !Sample_title = cy103_CD45pos_S178 !Sample_geo_accession = GSM3201673 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447677 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201674 !Sample_title = cy75_1CORE_CD45pos_S22 !Sample_geo_accession = GSM3201674 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447676 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201675 !Sample_title = cy105_CD45pos_S197 !Sample_geo_accession = GSM3201675 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447675 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201676 !Sample_title = cy105_CD45pos_S219 !Sample_geo_accession = GSM3201676 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447674 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201677 !Sample_title = cy75_1CORE_CD45pos_S43 !Sample_geo_accession = GSM3201677 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447679 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201678 !Sample_title = cy105_CD45pos_S205 !Sample_geo_accession = GSM3201678 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447678 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201679 !Sample_title = cy106_CD45pos_S330 !Sample_geo_accession = GSM3201679 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447749 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201680 !Sample_title = cy103_CD45pos_S100 !Sample_geo_accession = GSM3201680 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447748 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201681 !Sample_title = cy103_CD45pos_S143 !Sample_geo_accession = GSM3201681 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447747 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201682 !Sample_title = cy103_CD45pos_S164 !Sample_geo_accession = GSM3201682 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447746 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201683 !Sample_title = cy75_1CORE_CD45pos_S12 !Sample_geo_accession = GSM3201683 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447745 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201684 !Sample_title = cy103_CD45pos_S160 !Sample_geo_accession = GSM3201684 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447744 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201685 !Sample_title = cy75_1CORE_CD45pos_S31 !Sample_geo_accession = GSM3201685 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09447743 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201686 !Sample_title = cy103_CD45pos_S103 !Sample_geo_accession = GSM3201686 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448029 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201687 !Sample_title = cy75_1CORE_CD45pos_S92 !Sample_geo_accession = GSM3201687 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448028 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201688 !Sample_title = cy105_CD45pos_S253 !Sample_geo_accession = GSM3201688 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448027 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201689 !Sample_title = cy105_CD45pos_S276 !Sample_geo_accession = GSM3201689 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448026 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201690 !Sample_title = cy75_1CORE_CD45pos_S67 !Sample_geo_accession = GSM3201690 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448025 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201691 !Sample_title = cy75_1CORE_CD45pos_S10 !Sample_geo_accession = GSM3201691 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448024 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201692 !Sample_title = cy106_CD45pos_S303 !Sample_geo_accession = GSM3201692 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448023 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201693 !Sample_title = cy106_CD45pos_S348 !Sample_geo_accession = GSM3201693 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448022 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201694 !Sample_title = cy105_CD45pos_S198 !Sample_geo_accession = GSM3201694 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448021 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201695 !Sample_title = cy75_1CORE_CD45pos_S80 !Sample_geo_accession = GSM3201695 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448020 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201696 !Sample_title = cy75_1CORE_CD45pos_S36 !Sample_geo_accession = GSM3201696 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448019 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201697 !Sample_title = cy105_CD45pos_S203 !Sample_geo_accession = GSM3201697 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448018 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201698 !Sample_title = cy75_1CORE_CD45pos_S72 !Sample_geo_accession = GSM3201698 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448017 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201699 !Sample_title = cy106_CD45pos_S297 !Sample_geo_accession = GSM3201699 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448016 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201700 !Sample_title = cy105_CD45pos_S243 !Sample_geo_accession = GSM3201700 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448015 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201701 !Sample_title = cy105_CD45pos_S248 !Sample_geo_accession = GSM3201701 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448014 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201702 !Sample_title = cy75_1CORE_CD45pos_S59 !Sample_geo_accession = GSM3201702 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448013 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201703 !Sample_title = cy106_CD45pos_S380 !Sample_geo_accession = GSM3201703 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448009 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201704 !Sample_title = cy106_CD45pos_S323 !Sample_geo_accession = GSM3201704 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448008 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201705 !Sample_title = cy106_CD45pos_S343 !Sample_geo_accession = GSM3201705 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448007 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201706 !Sample_title = cy75_1CORE_CD45pos_S57 !Sample_geo_accession = GSM3201706 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448006 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201707 !Sample_title = cy75_1CORE_CD45pos_S86 !Sample_geo_accession = GSM3201707 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448012 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201708 !Sample_title = cy75_1CORE_CD45pos_S82 !Sample_geo_accession = GSM3201708 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448011 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201709 !Sample_title = cy75_1CORE_CD45pos_S19 !Sample_geo_accession = GSM3201709 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448010 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201710 !Sample_title = cy106_CD45pos_S360 !Sample_geo_accession = GSM3201710 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448005 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201711 !Sample_title = cy75_1CORE_CD45pos_S54 !Sample_geo_accession = GSM3201711 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448004 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201712 !Sample_title = cy105_CD45pos_S217 !Sample_geo_accession = GSM3201712 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448003 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201713 !Sample_title = cy75_1CORE_CD45pos_S26 !Sample_geo_accession = GSM3201713 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448002 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201714 !Sample_title = cy105_CD45pos_S281 !Sample_geo_accession = GSM3201714 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448066 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201715 !Sample_title = cy105_CD45pos_S195 !Sample_geo_accession = GSM3201715 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448065 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201716 !Sample_title = cy106_CD45pos_S312 !Sample_geo_accession = GSM3201716 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448064 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201717 !Sample_title = cy75_1CORE_CD45pos_S3 !Sample_geo_accession = GSM3201717 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448063 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201718 !Sample_title = cy75_1CORE_CD45pos_S29 !Sample_geo_accession = GSM3201718 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448062 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201719 !Sample_title = cy106_CD45pos_S345 !Sample_geo_accession = GSM3201719 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448061 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201720 !Sample_title = cy75_1CORE_CD45pos_S37 !Sample_geo_accession = GSM3201720 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09448060 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201721 !Sample_title = cy105_CD45pos_S216 !Sample_geo_accession = GSM3201721 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450968 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201722 !Sample_title = cy105_CD45pos_S233 !Sample_geo_accession = GSM3201722 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450967 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201723 !Sample_title = cy103_CD45pos_S166 !Sample_geo_accession = GSM3201723 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450966 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201724 !Sample_title = cy103_CD45pos_S126 !Sample_geo_accession = GSM3201724 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450965 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201725 !Sample_title = cy75_1CORE_CD45pos_S15 !Sample_geo_accession = GSM3201725 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451036 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201726 !Sample_title = cy75_1CORE_CD45pos_S46 !Sample_geo_accession = GSM3201726 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451035 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201727 !Sample_title = cy75_1CORE_CD45pos_S77 !Sample_geo_accession = GSM3201727 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451034 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201728 !Sample_title = cy105_CD45pos_S202 !Sample_geo_accession = GSM3201728 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451033 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201729 !Sample_title = cy106_CD45pos_S302 !Sample_geo_accession = GSM3201729 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451032 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201730 !Sample_title = cy103_CD45pos_S127 !Sample_geo_accession = GSM3201730 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451031 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201731 !Sample_title = cy103_CD45pos_S183 !Sample_geo_accession = GSM3201731 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451030 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201732 !Sample_title = cy75_1CORE_CD45pos_S89 !Sample_geo_accession = GSM3201732 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451029 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201733 !Sample_title = cy105_CD45pos_S208 !Sample_geo_accession = GSM3201733 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451028 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201734 !Sample_title = cy103_CD45pos_S121 !Sample_geo_accession = GSM3201734 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451027 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201735 !Sample_title = cy75_1CORE_CD45pos_S11 !Sample_geo_accession = GSM3201735 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451026 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201736 !Sample_title = cy103_CD45pos_S98 !Sample_geo_accession = GSM3201736 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451025 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201737 !Sample_title = cy103_CD45pos_S137 !Sample_geo_accession = GSM3201737 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451024 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201738 !Sample_title = cy75_1CORE_CD45pos_S96 !Sample_geo_accession = GSM3201738 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451023 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201739 !Sample_title = cy106_CD45pos_S342 !Sample_geo_accession = GSM3201739 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451022 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201740 !Sample_title = cy103_CD45pos_S135 !Sample_geo_accession = GSM3201740 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451021 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201741 !Sample_title = cy75_1CORE_CD45pos_S90 !Sample_geo_accession = GSM3201741 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451020 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201742 !Sample_title = cy106_CD45pos_S322 !Sample_geo_accession = GSM3201742 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451019 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201743 !Sample_title = cy105_CD45pos_S221 !Sample_geo_accession = GSM3201743 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451018 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201744 !Sample_title = cy105_CD45pos_S271 !Sample_geo_accession = GSM3201744 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451017 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201745 !Sample_title = cy103_CD45pos_S141 !Sample_geo_accession = GSM3201745 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451016 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201746 !Sample_title = cy103_CD45pos_S168 !Sample_geo_accession = GSM3201746 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451442 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201747 !Sample_title = cy103_CD45pos_S97 !Sample_geo_accession = GSM3201747 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451441 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201748 !Sample_title = cy75_1CORE_CD45pos_S39 !Sample_geo_accession = GSM3201748 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451440 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201749 !Sample_title = cy75_1CORE_CD45pos_S55 !Sample_geo_accession = GSM3201749 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451439 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201750 !Sample_title = cy106_CD45pos_S363 !Sample_geo_accession = GSM3201750 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451438 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201751 !Sample_title = cy103_CD45pos_S111 !Sample_geo_accession = GSM3201751 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451437 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201752 !Sample_title = cy103_CD45pos_S101 !Sample_geo_accession = GSM3201752 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451436 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201753 !Sample_title = cy75_1CORE_CD45pos_S32 !Sample_geo_accession = GSM3201753 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451435 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201754 !Sample_title = cy106_CD45pos_S321 !Sample_geo_accession = GSM3201754 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451509 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201755 !Sample_title = cy103_CD45pos_S132 !Sample_geo_accession = GSM3201755 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451508 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201756 !Sample_title = cy106_CD45pos_S307 !Sample_geo_accession = GSM3201756 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451507 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201757 !Sample_title = cy105_CD45pos_S251 !Sample_geo_accession = GSM3201757 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451506 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201758 !Sample_title = cy105_CD45pos_S280 !Sample_geo_accession = GSM3201758 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451505 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201759 !Sample_title = cy75_1CORE_CD45pos_S94 !Sample_geo_accession = GSM3201759 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451504 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201760 !Sample_title = cy103_CD45pos_S163 !Sample_geo_accession = GSM3201760 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451503 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201761 !Sample_title = cy106_CD45pos_S347 !Sample_geo_accession = GSM3201761 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451502 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201762 !Sample_title = cy75_1CORE_CD45pos_S30 !Sample_geo_accession = GSM3201762 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451501 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201763 !Sample_title = cy106_CD45pos_S316 !Sample_geo_accession = GSM3201763 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451498 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201764 !Sample_title = cy75_1CORE_CD45pos_S69 !Sample_geo_accession = GSM3201764 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451497 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201765 !Sample_title = cy105_CD45pos_S237 !Sample_geo_accession = GSM3201765 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451496 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201766 !Sample_title = cy103_CD45pos_S148 !Sample_geo_accession = GSM3201766 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451495 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201767 !Sample_title = cy75_1CORE_CD45pos_S20 !Sample_geo_accession = GSM3201767 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451500 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201768 !Sample_title = cy105_CD45pos_S213 !Sample_geo_accession = GSM3201768 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451499 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201769 !Sample_title = cy103_CD45pos_S185 !Sample_geo_accession = GSM3201769 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451494 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201770 !Sample_title = cy103_CD45pos_S109 !Sample_geo_accession = GSM3201770 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451493 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201771 !Sample_title = cy105_CD45pos_S287 !Sample_geo_accession = GSM3201771 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451492 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201772 !Sample_title = cy103_CD45pos_S177 !Sample_geo_accession = GSM3201772 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451491 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201773 !Sample_title = cy103_CD45pos_S189 !Sample_geo_accession = GSM3201773 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451490 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201774 !Sample_title = cy105_CD45pos_S264 !Sample_geo_accession = GSM3201774 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451489 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201775 !Sample_title = cy75_1CORE_CD45pos_S79 !Sample_geo_accession = GSM3201775 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451488 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201776 !Sample_title = cy103_CD45pos_S191 !Sample_geo_accession = GSM3201776 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451117 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201777 !Sample_title = cy105_CD45pos_S229 !Sample_geo_accession = GSM3201777 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451116 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201778 !Sample_title = cy105_CD45pos_S259 !Sample_geo_accession = GSM3201778 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451115 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201779 !Sample_title = cy75_1CORE_CD45pos_S83 !Sample_geo_accession = GSM3201779 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451114 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201780 !Sample_title = cy103_CD45pos_S157 !Sample_geo_accession = GSM3201780 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451181 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201781 !Sample_title = cy75_1CORE_CD45pos_S27 !Sample_geo_accession = GSM3201781 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451180 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201782 !Sample_title = cy103_CD45pos_S102 !Sample_geo_accession = GSM3201782 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451179 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201783 !Sample_title = cy103_CD45pos_S117 !Sample_geo_accession = GSM3201783 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451178 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201784 !Sample_title = cy105_CD45pos_S223 !Sample_geo_accession = GSM3201784 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451177 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201785 !Sample_title = cy106_CD45pos_S349 !Sample_geo_accession = GSM3201785 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451176 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201786 !Sample_title = cy103_CD45pos_S165 !Sample_geo_accession = GSM3201786 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451175 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201787 !Sample_title = cy105_CD45pos_S274 !Sample_geo_accession = GSM3201787 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451174 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201788 !Sample_title = cy75_1CORE_CD45pos_S1 !Sample_geo_accession = GSM3201788 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451173 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201789 !Sample_title = cy102_CD45neg_CD90neg_S94 !Sample_geo_accession = GSM3201789 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451172 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201790 !Sample_title = cy102_CD45neg_CD90neg_S2 !Sample_geo_accession = GSM3201790 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451171 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201791 !Sample_title = cy102_CD45neg_CD90neg_S6 !Sample_geo_accession = GSM3201791 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451170 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201792 !Sample_title = cy110_CD45neg_CD90neg_S221 !Sample_geo_accession = GSM3201792 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451169 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201793 !Sample_title = cy110_CD45neg_CD90neg_S251 !Sample_geo_accession = GSM3201793 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451168 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201794 !Sample_title = cy103_CD45neg_CD90neg_S104 !Sample_geo_accession = GSM3201794 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451164 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201795 !Sample_title = cy102_CD45neg_CD90neg_S27 !Sample_geo_accession = GSM3201795 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451163 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201796 !Sample_title = cy110_CD45neg_CD90neg_S201 !Sample_geo_accession = GSM3201796 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451162 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201797 !Sample_title = cy103_CD45neg_CD90neg_S106 !Sample_geo_accession = GSM3201797 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451167 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201798 !Sample_title = cy110_CD45neg_CD90neg_S206 !Sample_geo_accession = GSM3201798 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451166 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201799 !Sample_title = cy103_CD45neg_CD90neg_S149 !Sample_geo_accession = GSM3201799 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451165 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201800 !Sample_title = cy103_CD45neg_CD90neg_S131 !Sample_geo_accession = GSM3201800 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451161 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201801 !Sample_title = cy102_CD45neg_CD90neg_S82 !Sample_geo_accession = GSM3201801 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451160 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201802 !Sample_title = cy103_CD45neg_CD90neg_S116 !Sample_geo_accession = GSM3201802 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451159 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201803 !Sample_title = cy103_CD45neg_CD90neg_S126 !Sample_geo_accession = GSM3201803 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451158 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201804 !Sample_title = cy102_CD45neg_CD90neg_S33 !Sample_geo_accession = GSM3201804 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451157 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201805 !Sample_title = cy103_CD45neg_CD90neg_S174 !Sample_geo_accession = GSM3201805 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451156 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201806 !Sample_title = cy102_CD45neg_CD90neg_S51 !Sample_geo_accession = GSM3201806 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450433 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201807 !Sample_title = cy102_CD45neg_CD90neg_S22 !Sample_geo_accession = GSM3201807 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450432 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201808 !Sample_title = cy103_CD45neg_CD90neg_S169 !Sample_geo_accession = GSM3201808 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450431 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201809 !Sample_title = cy102_CD45neg_CD90neg_S91 !Sample_geo_accession = GSM3201809 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450430 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201810 !Sample_title = cy112_CD45neg_CD90neg_S323 !Sample_geo_accession = GSM3201810 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450429 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201811 !Sample_title = cy103_CD45neg_CD90neg_S108 !Sample_geo_accession = GSM3201811 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450428 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201812 !Sample_title = cy102_CD45neg_CD90neg_S7 !Sample_geo_accession = GSM3201812 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450427 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201813 !Sample_title = cy112_CD45neg_CD90neg_S337 !Sample_geo_accession = GSM3201813 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450426 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201814 !Sample_title = cy103_CD45neg_CD90neg_S166 !Sample_geo_accession = GSM3201814 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450425 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201815 !Sample_title = cy103_CD45neg_CD90neg_S164 !Sample_geo_accession = GSM3201815 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450424 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201816 !Sample_title = cy110_CD45neg_CD90neg_S286 !Sample_geo_accession = GSM3201816 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450423 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201817 !Sample_title = cy102_CD45neg_CD90neg_S62 !Sample_geo_accession = GSM3201817 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450422 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201818 !Sample_title = cy110_CD45neg_CD90neg_S208 !Sample_geo_accession = GSM3201818 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450421 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201819 !Sample_title = cy110_CD45neg_CD90neg_S256 !Sample_geo_accession = GSM3201819 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450420 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201820 !Sample_title = cy103_CD45neg_CD90neg_S185 !Sample_geo_accession = GSM3201820 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450419 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201821 !Sample_title = cy102_CD45neg_CD90neg_S93 !Sample_geo_accession = GSM3201821 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450418 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201822 !Sample_title = cy110_CD45neg_CD90neg_S266 !Sample_geo_accession = GSM3201822 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450417 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201823 !Sample_title = cy102_CD45neg_CD90neg_S80 !Sample_geo_accession = GSM3201823 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450416 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201824 !Sample_title = cy112_CD45neg_CD90neg_S340 !Sample_geo_accession = GSM3201824 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450415 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201825 !Sample_title = cy103_CD45neg_CD90neg_S160 !Sample_geo_accession = GSM3201825 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450414 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201826 !Sample_title = cy103_CD45neg_CD90neg_S154 !Sample_geo_accession = GSM3201826 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450488 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201827 !Sample_title = cy102_CD45neg_CD90neg_S75 !Sample_geo_accession = GSM3201827 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450487 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201828 !Sample_title = cy103_CD45neg_CD90neg_S139 !Sample_geo_accession = GSM3201828 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450486 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201829 !Sample_title = cy103_CD45neg_CD90neg_S153 !Sample_geo_accession = GSM3201829 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450485 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201830 !Sample_title = cy110_CD45neg_CD90neg_S249 !Sample_geo_accession = GSM3201830 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450482 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201831 !Sample_title = cy110_CD45neg_CD90neg_S248 !Sample_geo_accession = GSM3201831 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450481 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201832 !Sample_title = cy110_CD45neg_CD90neg_S200 !Sample_geo_accession = GSM3201832 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450480 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201833 !Sample_title = cy102_CD45neg_CD90neg_S55 !Sample_geo_accession = GSM3201833 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450479 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201834 !Sample_title = cy103_CD45neg_CD90neg_S182 !Sample_geo_accession = GSM3201834 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450484 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201835 !Sample_title = cy102_CD45neg_CD90neg_S11 !Sample_geo_accession = GSM3201835 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450483 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201836 !Sample_title = cy110_CD45neg_CD90neg_S241 !Sample_geo_accession = GSM3201836 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450478 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201837 !Sample_title = cy102_CD45neg_CD90neg_S4 !Sample_geo_accession = GSM3201837 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450477 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201838 !Sample_title = cy103_CD45neg_CD90neg_S133 !Sample_geo_accession = GSM3201838 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450476 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201839 !Sample_title = cy110_CD45neg_CD90neg_S229 !Sample_geo_accession = GSM3201839 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450475 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201840 !Sample_title = cy103_CD45neg_CD90neg_S138 !Sample_geo_accession = GSM3201840 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450474 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201841 !Sample_title = cy102_CD45neg_CD90neg_S74 !Sample_geo_accession = GSM3201841 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450473 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201842 !Sample_title = cy102_CD45neg_CD90neg_S73 !Sample_geo_accession = GSM3201842 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450472 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201843 !Sample_title = cy102_CD45neg_CD90neg_S34 !Sample_geo_accession = GSM3201843 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450471 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201844 !Sample_title = cy102_CD45neg_CD90neg_S24 !Sample_geo_accession = GSM3201844 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450470 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201845 !Sample_title = cy103_CD45neg_CD90neg_S161 !Sample_geo_accession = GSM3201845 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450469 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201846 !Sample_title = cy103_CD45neg_CD90neg_S125 !Sample_geo_accession = GSM3201846 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450468 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201847 !Sample_title = cy103_CD45neg_CD90neg_S141 !Sample_geo_accession = GSM3201847 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450467 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201848 !Sample_title = cy110_CD45neg_CD90neg_S238 !Sample_geo_accession = GSM3201848 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450466 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201849 !Sample_title = cy110_CD45neg_CD90neg_S209 !Sample_geo_accession = GSM3201849 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450465 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201850 !Sample_title = cy103_CD45neg_CD90neg_S150 !Sample_geo_accession = GSM3201850 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450464 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201851 !Sample_title = cy102_CD45neg_CD90neg_S20 !Sample_geo_accession = GSM3201851 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450463 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201852 !Sample_title = cy102_CD45neg_CD90neg_S29 !Sample_geo_accession = GSM3201852 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450462 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201853 !Sample_title = cy110_CD45neg_CD90neg_S193 !Sample_geo_accession = GSM3201853 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450461 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201854 !Sample_title = cy110_CD45neg_CD90neg_S261 !Sample_geo_accession = GSM3201854 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450460 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201855 !Sample_title = cy102_CD45neg_CD90neg_S35 !Sample_geo_accession = GSM3201855 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450459 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201856 !Sample_title = cy103_CD45neg_CD90neg_S123 !Sample_geo_accession = GSM3201856 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450458 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201857 !Sample_title = cy102_CD45neg_CD90neg_S9 !Sample_geo_accession = GSM3201857 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450457 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201858 !Sample_title = cy103_CD45neg_CD90neg_S173 !Sample_geo_accession = GSM3201858 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450456 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201859 !Sample_title = cy112_CD45neg_CD90neg_S310 !Sample_geo_accession = GSM3201859 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450455 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201860 !Sample_title = cy102_CD45neg_CD90neg_S81 !Sample_geo_accession = GSM3201860 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450529 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201861 !Sample_title = cy110_CD45neg_CD90neg_S198 !Sample_geo_accession = GSM3201861 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450528 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201862 !Sample_title = cy102_CD45neg_CD90neg_S66 !Sample_geo_accession = GSM3201862 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450527 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201863 !Sample_title = cy102_CD45neg_CD90neg_S68 !Sample_geo_accession = GSM3201863 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450526 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201864 !Sample_title = cy102_CD45neg_CD90neg_S43 !Sample_geo_accession = GSM3201864 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450454 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201865 !Sample_title = cy102_CD45neg_CD90neg_S23 !Sample_geo_accession = GSM3201865 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450530 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201866 !Sample_title = cy102_CD45neg_CD90neg_S57 !Sample_geo_accession = GSM3201866 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450796 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201867 !Sample_title = cy103_CD45neg_CD90neg_S172 !Sample_geo_accession = GSM3201867 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450795 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201868 !Sample_title = cy110_CD45neg_CD90neg_S232 !Sample_geo_accession = GSM3201868 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450793 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201869 !Sample_title = cy103_CD45neg_CD90neg_S181 !Sample_geo_accession = GSM3201869 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450792 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201870 !Sample_title = cy110_CD45neg_CD90neg_S250 !Sample_geo_accession = GSM3201870 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450791 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201871 !Sample_title = cy103_CD45neg_CD90neg_S135 !Sample_geo_accession = GSM3201871 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450790 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201872 !Sample_title = cy110_CD45neg_CD90neg_S246 !Sample_geo_accession = GSM3201872 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450789 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201873 !Sample_title = cy110_CD45neg_CD90neg_S216 !Sample_geo_accession = GSM3201873 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450788 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201874 !Sample_title = cy103_CD45neg_CD90neg_S180 !Sample_geo_accession = GSM3201874 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450787 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201875 !Sample_title = cy110_CD45neg_CD90neg_S278 !Sample_geo_accession = GSM3201875 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450786 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201876 !Sample_title = cy103_CD45neg_CD90neg_S122 !Sample_geo_accession = GSM3201876 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450785 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201877 !Sample_title = cy110_CD45neg_CD90neg_S287 !Sample_geo_accession = GSM3201877 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450784 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201878 !Sample_title = cy103_CD45neg_CD90neg_S140 !Sample_geo_accession = GSM3201878 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450783 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201879 !Sample_title = cy102_CD45neg_CD90neg_S30 !Sample_geo_accession = GSM3201879 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450782 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201880 !Sample_title = cy102_CD45neg_CD90neg_S15 !Sample_geo_accession = GSM3201880 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450781 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201881 !Sample_title = cy103_CD45neg_CD90neg_S119 !Sample_geo_accession = GSM3201881 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450780 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201882 !Sample_title = cy110_CD45neg_CD90neg_S218 !Sample_geo_accession = GSM3201882 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450779 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201883 !Sample_title = cy102_CD45neg_CD90neg_S1 !Sample_geo_accession = GSM3201883 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450857 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201884 !Sample_title = cy103_CD45neg_CD90neg_S128 !Sample_geo_accession = GSM3201884 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450856 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201885 !Sample_title = cy102_CD45neg_CD90neg_S64 !Sample_geo_accession = GSM3201885 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450855 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201886 !Sample_title = cy103_CD45neg_CD90neg_S99 !Sample_geo_accession = GSM3201886 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450854 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201887 !Sample_title = cy102_CD45neg_CD90neg_S83 !Sample_geo_accession = GSM3201887 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450853 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201888 !Sample_title = cy110_CD45neg_CD90neg_S194 !Sample_geo_accession = GSM3201888 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450852 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201889 !Sample_title = cy110_CD45neg_CD90neg_S237 !Sample_geo_accession = GSM3201889 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450851 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201890 !Sample_title = cy102_CD45neg_CD90neg_S84 !Sample_geo_accession = GSM3201890 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450850 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201891 !Sample_title = cy103_CD45neg_CD90neg_S112 !Sample_geo_accession = GSM3201891 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450849 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201892 !Sample_title = cy102_CD45neg_CD90neg_S40 !Sample_geo_accession = GSM3201892 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450848 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201893 !Sample_title = cy103_CD45neg_CD90neg_S163 !Sample_geo_accession = GSM3201893 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450847 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201894 !Sample_title = cy103_CD45neg_CD90neg_S178 !Sample_geo_accession = GSM3201894 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450846 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201895 !Sample_title = cy103_CD45neg_CD90neg_S130 !Sample_geo_accession = GSM3201895 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450845 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201896 !Sample_title = cy103_CD45neg_CD90neg_S168 !Sample_geo_accession = GSM3201896 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450810 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201897 !Sample_title = cy103_CD45neg_CD90neg_S184 !Sample_geo_accession = GSM3201897 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450809 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201898 !Sample_title = cy103_CD45neg_CD90neg_S100 !Sample_geo_accession = GSM3201898 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450808 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201899 !Sample_title = cy102_CD45neg_CD90neg_S95 !Sample_geo_accession = GSM3201899 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450807 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201900 !Sample_title = cy103_CD45neg_CD90neg_S121 !Sample_geo_accession = GSM3201900 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450806 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201901 !Sample_title = cy110_CD45neg_CD90neg_S223 !Sample_geo_accession = GSM3201901 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450805 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201902 !Sample_title = cy103_CD45neg_CD90neg_S189 !Sample_geo_accession = GSM3201902 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450804 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201903 !Sample_title = cy103_CD45neg_CD90neg_S137 !Sample_geo_accession = GSM3201903 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450803 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201904 !Sample_title = cy102_CD45neg_CD90neg_S92 !Sample_geo_accession = GSM3201904 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450802 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201905 !Sample_title = cy112_CD45neg_CD90neg_S341 !Sample_geo_accession = GSM3201905 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450801 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201906 !Sample_title = cy110_CD45neg_CD90neg_S276 !Sample_geo_accession = GSM3201906 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450800 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201907 !Sample_title = cy103_CD45neg_CD90neg_S136 !Sample_geo_accession = GSM3201907 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450799 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201908 !Sample_title = cy112_CD45neg_CD90neg_S309 !Sample_geo_accession = GSM3201908 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450798 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201909 !Sample_title = cy102_CD45neg_CD90neg_S53 !Sample_geo_accession = GSM3201909 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450797 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201910 !Sample_title = cy102_CD45neg_CD90neg_S48 !Sample_geo_accession = GSM3201910 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450757 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201911 !Sample_title = cy110_CD45neg_CD90neg_S267 !Sample_geo_accession = GSM3201911 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450756 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201912 !Sample_title = cy102_CD45neg_CD90neg_S49 !Sample_geo_accession = GSM3201912 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450755 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201913 !Sample_title = cy110_CD45neg_CD90neg_S255 !Sample_geo_accession = GSM3201913 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450754 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201914 !Sample_title = cy103_CD45neg_CD90neg_S162 !Sample_geo_accession = GSM3201914 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450753 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201915 !Sample_title = cy102_CD45neg_CD90neg_S54 !Sample_geo_accession = GSM3201915 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450752 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201916 !Sample_title = cy103_CD45neg_CD90neg_S109 !Sample_geo_accession = GSM3201916 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450751 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201917 !Sample_title = cy103_CD45neg_CD90neg_S188 !Sample_geo_accession = GSM3201917 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450750 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201918 !Sample_title = cy103_CD45neg_CD90neg_S145 !Sample_geo_accession = GSM3201918 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450749 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201919 !Sample_title = cy102_CD45neg_CD90neg_S69 !Sample_geo_accession = GSM3201919 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450748 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201920 !Sample_title = cy102_CD45neg_CD90neg_S60 !Sample_geo_accession = GSM3201920 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450827 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201921 !Sample_title = cy110_CD45neg_CD90neg_S254 !Sample_geo_accession = GSM3201921 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450826 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201922 !Sample_title = cy102_CD45neg_CD90neg_S17 !Sample_geo_accession = GSM3201922 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450825 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201923 !Sample_title = cy103_CD45neg_CD90neg_S190 !Sample_geo_accession = GSM3201923 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450824 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201924 !Sample_title = cy102_CD45neg_CD90neg_S58 !Sample_geo_accession = GSM3201924 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450747 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201925 !Sample_title = cy110_CD45neg_CD90neg_S252 !Sample_geo_accession = GSM3201925 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450746 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201926 !Sample_title = cy102_CD45neg_CD90neg_S65 !Sample_geo_accession = GSM3201926 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450745 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201927 !Sample_title = cy102_CD45neg_CD90neg_S13 !Sample_geo_accession = GSM3201927 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450823 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201928 !Sample_title = cy102_CD45neg_CD90neg_S90 !Sample_geo_accession = GSM3201928 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450822 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201929 !Sample_title = cy102_CD45neg_CD90neg_S32 !Sample_geo_accession = GSM3201929 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450821 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201930 !Sample_title = cy110_CD45neg_CD90neg_S247 !Sample_geo_accession = GSM3201930 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450820 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201931 !Sample_title = cy102_CD45neg_CD90neg_S19 !Sample_geo_accession = GSM3201931 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450819 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201932 !Sample_title = cy110_CD45neg_CD90neg_S205 !Sample_geo_accession = GSM3201932 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450818 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201933 !Sample_title = cy102_CD45neg_CD90neg_S46 !Sample_geo_accession = GSM3201933 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450817 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201934 !Sample_title = cy102_CD45neg_CD90neg_S61 !Sample_geo_accession = GSM3201934 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450816 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201935 !Sample_title = cy102_CD45neg_CD90neg_S31 !Sample_geo_accession = GSM3201935 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450815 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201936 !Sample_title = cy103_CD45neg_CD90neg_S152 !Sample_geo_accession = GSM3201936 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450814 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201937 !Sample_title = cy103_CD45neg_CD90neg_S118 !Sample_geo_accession = GSM3201937 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450813 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201938 !Sample_title = cy112_CD45neg_CD90neg_S330 !Sample_geo_accession = GSM3201938 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450812 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201939 !Sample_title = cy103_CD45neg_CD90neg_S165 !Sample_geo_accession = GSM3201939 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450811 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201940 !Sample_title = cy110_CD45neg_CD90neg_S213 !Sample_geo_accession = GSM3201940 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450879 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201941 !Sample_title = cy110_CD45neg_CD90neg_S285 !Sample_geo_accession = GSM3201941 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450878 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201942 !Sample_title = cy103_CD45neg_CD90neg_S157 !Sample_geo_accession = GSM3201942 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450877 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201943 !Sample_title = cy102_CD45neg_CD90neg_S36 !Sample_geo_accession = GSM3201943 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450876 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201944 !Sample_title = cy103_CD45neg_CD90neg_S114 !Sample_geo_accession = GSM3201944 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450875 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201945 !Sample_title = cy102_CD45neg_CD90neg_S78 !Sample_geo_accession = GSM3201945 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450874 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201946 !Sample_title = cy112_CD45neg_CD90neg_S291 !Sample_geo_accession = GSM3201946 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450873 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201947 !Sample_title = cy103_CD45neg_CD90neg_S120 !Sample_geo_accession = GSM3201947 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450872 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201948 !Sample_title = cy102_CD45neg_CD90neg_S76 !Sample_geo_accession = GSM3201948 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450871 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201949 !Sample_title = cy103_CD45neg_CD90neg_S159 !Sample_geo_accession = GSM3201949 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450870 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201950 !Sample_title = cy110_CD45neg_CD90neg_S282 !Sample_geo_accession = GSM3201950 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450869 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201951 !Sample_title = cy110_CD45neg_CD90neg_S235 !Sample_geo_accession = GSM3201951 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450863 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201952 !Sample_title = cy110_CD45neg_CD90neg_S203 !Sample_geo_accession = GSM3201952 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450862 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201953 !Sample_title = cy103_CD45neg_CD90neg_S134 !Sample_geo_accession = GSM3201953 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450861 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201954 !Sample_title = cy103_CD45neg_CD90neg_S115 !Sample_geo_accession = GSM3201954 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450868 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201955 !Sample_title = cy102_CD45neg_CD90neg_S88 !Sample_geo_accession = GSM3201955 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450867 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201956 !Sample_title = cy103_CD45neg_CD90neg_S97 !Sample_geo_accession = GSM3201956 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450866 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201957 !Sample_title = cy110_CD45neg_CD90neg_S202 !Sample_geo_accession = GSM3201957 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450864 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201958 !Sample_title = cy110_CD45neg_CD90neg_S197 !Sample_geo_accession = GSM3201958 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450860 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201959 !Sample_title = cy102_CD45neg_CD90neg_S5 !Sample_geo_accession = GSM3201959 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450859 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201960 !Sample_title = cy102_CD45neg_CD90neg_S59 !Sample_geo_accession = GSM3201960 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450858 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201961 !Sample_title = cy102_CD45neg_CD90neg_S77 !Sample_geo_accession = GSM3201961 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450927 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201962 !Sample_title = cy102_CD45neg_CD90neg_S37 !Sample_geo_accession = GSM3201962 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450926 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201963 !Sample_title = cy103_CD45neg_CD90neg_S105 !Sample_geo_accession = GSM3201963 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450925 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201964 !Sample_title = cy103_CD45neg_CD90neg_S151 !Sample_geo_accession = GSM3201964 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450924 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201965 !Sample_title = cy102_CD45neg_CD90neg_S79 !Sample_geo_accession = GSM3201965 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450923 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201966 !Sample_title = cy110_CD45neg_CD90neg_S288 !Sample_geo_accession = GSM3201966 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450922 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201967 !Sample_title = cy103_CD45neg_CD90neg_S143 !Sample_geo_accession = GSM3201967 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450921 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201968 !Sample_title = cy102_CD45neg_CD90neg_S85 !Sample_geo_accession = GSM3201968 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450920 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201969 !Sample_title = cy103_CD45neg_CD90neg_S179 !Sample_geo_accession = GSM3201969 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450919 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201970 !Sample_title = cy102_CD45neg_CD90neg_S86 !Sample_geo_accession = GSM3201970 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450930 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201971 !Sample_title = cy102_CD45neg_CD90neg_S47 !Sample_geo_accession = GSM3201971 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450929 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201972 !Sample_title = cy102_CD45neg_CD90neg_S18 !Sample_geo_accession = GSM3201972 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450928 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201973 !Sample_title = cy110_CD45neg_CD90neg_S271 !Sample_geo_accession = GSM3201973 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451000 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201974 !Sample_title = cy103_CD45neg_CD90neg_S186 !Sample_geo_accession = GSM3201974 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450999 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201975 !Sample_title = cy112_CD45neg_CD90neg_S297 !Sample_geo_accession = GSM3201975 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450998 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201976 !Sample_title = cy112_CD45neg_CD90neg_S348 !Sample_geo_accession = GSM3201976 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450997 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201977 !Sample_title = cy102_CD45neg_CD90neg_S45 !Sample_geo_accession = GSM3201977 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450996 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201978 !Sample_title = cy112_CD45neg_CD90neg_S320 !Sample_geo_accession = GSM3201978 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450995 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201979 !Sample_title = cy102_CD45neg_CD90neg_S42 !Sample_geo_accession = GSM3201979 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450994 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201980 !Sample_title = cy110_CD45neg_CD90neg_S243 !Sample_geo_accession = GSM3201980 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450993 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201981 !Sample_title = cy112_CD45neg_CD90neg_S314 !Sample_geo_accession = GSM3201981 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450992 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201982 !Sample_title = cy102_CD45neg_CD90neg_S89 !Sample_geo_accession = GSM3201982 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450991 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201983 !Sample_title = cy102_CD45neg_CD90neg_S16 !Sample_geo_accession = GSM3201983 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450990 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201984 !Sample_title = cy102_CD45neg_CD90neg_S71 !Sample_geo_accession = GSM3201984 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450989 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201985 !Sample_title = cy110_CD45neg_CD90neg_S226 !Sample_geo_accession = GSM3201985 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450988 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201986 !Sample_title = cy103_CD45neg_CD90neg_S110 !Sample_geo_accession = GSM3201986 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450987 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201987 !Sample_title = cy110_CD45neg_CD90neg_S280 !Sample_geo_accession = GSM3201987 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450986 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201988 !Sample_title = cy103_CD45neg_CD90neg_S155 !Sample_geo_accession = GSM3201988 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450985 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201989 !Sample_title = cy103_CD45neg_CD90neg_S187 !Sample_geo_accession = GSM3201989 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450984 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201990 !Sample_title = cy110_CD45neg_CD90neg_S225 !Sample_geo_accession = GSM3201990 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450983 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201991 !Sample_title = cy102_CD45neg_CD90neg_S72 !Sample_geo_accession = GSM3201991 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450982 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201992 !Sample_title = cy102_CD45neg_CD90neg_S26 !Sample_geo_accession = GSM3201992 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450981 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201993 !Sample_title = cy102_CD45neg_CD90neg_S50 !Sample_geo_accession = GSM3201993 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450980 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201994 !Sample_title = cy110_CD45neg_CD90neg_S212 !Sample_geo_accession = GSM3201994 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450979 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201995 !Sample_title = cy103_CD45neg_CD90neg_S127 !Sample_geo_accession = GSM3201995 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450978 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201996 !Sample_title = cy102_CD45neg_CD90neg_S14 !Sample_geo_accession = GSM3201996 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450977 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201997 !Sample_title = cy103_CD45neg_CD90neg_S107 !Sample_geo_accession = GSM3201997 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450976 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201998 !Sample_title = cy103_CD45neg_CD90neg_S101 !Sample_geo_accession = GSM3201998 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450975 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3201999 !Sample_title = cy103_CD45neg_CD90neg_S183 !Sample_geo_accession = GSM3201999 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450974 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202000 !Sample_title = cy103_CD45neg_CD90neg_S111 !Sample_geo_accession = GSM3202000 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452942 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202001 !Sample_title = cy103_CD45neg_CD90neg_S129 !Sample_geo_accession = GSM3202001 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452941 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202002 !Sample_title = cy112_CD45neg_CD90neg_S299 !Sample_geo_accession = GSM3202002 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452940 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202003 !Sample_title = cy110_CD45neg_CD90neg_S273 !Sample_geo_accession = GSM3202003 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452939 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202004 !Sample_title = cy102_CD45neg_CD90neg_S21 !Sample_geo_accession = GSM3202004 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452938 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202005 !Sample_title = cy103_CD45neg_CD90neg_S170 !Sample_geo_accession = GSM3202005 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452937 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202006 !Sample_title = cy110_CD45neg_CD90neg_S264 !Sample_geo_accession = GSM3202006 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452936 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202007 !Sample_title = cy103_CD45neg_CD90neg_S156 !Sample_geo_accession = GSM3202007 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452935 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202008 !Sample_title = cy110_CD45neg_CD90neg_S275 !Sample_geo_accession = GSM3202008 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452934 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202009 !Sample_title = cy110_CD45neg_CD90neg_S277 !Sample_geo_accession = GSM3202009 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452933 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202010 !Sample_title = cy102_CD45neg_CD90neg_S67 !Sample_geo_accession = GSM3202010 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452932 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202011 !Sample_title = cy110_CD45neg_CD90neg_S242 !Sample_geo_accession = GSM3202011 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452931 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202012 !Sample_title = cy102_CD45neg_CD90neg_S63 !Sample_geo_accession = GSM3202012 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452925 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202013 !Sample_title = cy110_CD45neg_CD90neg_S268 !Sample_geo_accession = GSM3202013 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452924 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202014 !Sample_title = cy102_CD45neg_CD90neg_S28 !Sample_geo_accession = GSM3202014 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452930 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202015 !Sample_title = cy112_CD45neg_CD90neg_S358 !Sample_geo_accession = GSM3202015 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452929 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202016 !Sample_title = cy110_CD45neg_CD90neg_S234 !Sample_geo_accession = GSM3202016 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452928 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202017 !Sample_title = cy75_1B_CD45pos_S32 !Sample_geo_accession = GSM3202017 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452927 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202018 !Sample_title = cy75_1B_CORE_CD45neg_S146 !Sample_geo_accession = GSM3202018 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452926 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202019 !Sample_title = cy106_CD45neg_S330 !Sample_geo_accession = GSM3202019 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452923 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202020 !Sample_title = cy75_1B_CD45pos_S37 !Sample_geo_accession = GSM3202020 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452922 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202021 !Sample_title = cy106_CD45neg_S293 !Sample_geo_accession = GSM3202021 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452965 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202022 !Sample_title = cy75_1B_CD45pos_S95 !Sample_geo_accession = GSM3202022 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452964 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202023 !Sample_title = cy105_CD45neg_S220 !Sample_geo_accession = GSM3202023 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452963 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202024 !Sample_title = cy75_1B_CD45pos_S22 !Sample_geo_accession = GSM3202024 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452962 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202025 !Sample_title = cy75_1B_CD45pos_S46 !Sample_geo_accession = GSM3202025 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452961 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202026 !Sample_title = cy75_1B_CD45pos_S36 !Sample_geo_accession = GSM3202026 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452960 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202027 !Sample_title = cy75_1B_CD45pos_S71 !Sample_geo_accession = GSM3202027 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452959 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202028 !Sample_title = cy75_1B_CORE_CD45neg_S190 !Sample_geo_accession = GSM3202028 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452958 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202029 !Sample_title = cy105_CD45neg_S233 !Sample_geo_accession = GSM3202029 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452595 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202030 !Sample_title = cy106_CD45neg_S376 !Sample_geo_accession = GSM3202030 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452594 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202031 !Sample_title = cy106_CD45neg_S370 !Sample_geo_accession = GSM3202031 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452593 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202032 !Sample_title = cy75_1B_CORE_CD45neg_S115 !Sample_geo_accession = GSM3202032 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452592 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202033 !Sample_title = cy105_CD45neg_S288 !Sample_geo_accession = GSM3202033 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452591 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202034 !Sample_title = cy75_1B_CD45pos_S2 !Sample_geo_accession = GSM3202034 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452590 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202035 !Sample_title = cy75_1B_CD45pos_S63 !Sample_geo_accession = GSM3202035 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452589 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202036 !Sample_title = cy105_CD45neg_S283 !Sample_geo_accession = GSM3202036 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452588 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202037 !Sample_title = cy75_1B_CD45pos_S35 !Sample_geo_accession = GSM3202037 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452587 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202038 !Sample_title = cy106_CD45neg_S348 !Sample_geo_accession = GSM3202038 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452586 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202039 !Sample_title = cy105_CD45neg_S216 !Sample_geo_accession = GSM3202039 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452585 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202040 !Sample_title = cy75_1B_CD45pos_S80 !Sample_geo_accession = GSM3202040 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452584 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202041 !Sample_title = cy105_CD45neg_S277 !Sample_geo_accession = GSM3202041 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452583 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202042 !Sample_title = cy75_1B_CD45pos_S93 !Sample_geo_accession = GSM3202042 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452582 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202043 !Sample_title = cy75_1B_CD45pos_S90 !Sample_geo_accession = GSM3202043 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452581 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202044 !Sample_title = cy105_CD45neg_S236 !Sample_geo_accession = GSM3202044 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452580 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202045 !Sample_title = cy106_CD45neg_S360 !Sample_geo_accession = GSM3202045 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452579 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202046 !Sample_title = cy106_CD45neg_S383 !Sample_geo_accession = GSM3202046 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452578 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202047 !Sample_title = cy75_1B_CD45pos_S64 !Sample_geo_accession = GSM3202047 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452577 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202048 !Sample_title = cy75_1B_CORE_CD45neg_S158 !Sample_geo_accession = GSM3202048 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452576 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202049 !Sample_title = cy75_1B_CORE_CD45neg_S187 !Sample_geo_accession = GSM3202049 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452575 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202050 !Sample_title = cy75_1B_CD45pos_S85 !Sample_geo_accession = GSM3202050 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452574 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202051 !Sample_title = cy106_CD45neg_S332 !Sample_geo_accession = GSM3202051 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452573 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202052 !Sample_title = cy75_1B_CD45pos_S87 !Sample_geo_accession = GSM3202052 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452572 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202053 !Sample_title = cy75_1B_CD45pos_S96 !Sample_geo_accession = GSM3202053 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452571 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202054 !Sample_title = cy75_1B_CD45pos_S14 !Sample_geo_accession = GSM3202054 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452570 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202055 !Sample_title = cy106_CD45neg_S335 !Sample_geo_accession = GSM3202055 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452640 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202056 !Sample_title = cy105_CD45neg_S214 !Sample_geo_accession = GSM3202056 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452639 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202057 !Sample_title = cy75_1B_CORE_CD45neg_S181 !Sample_geo_accession = GSM3202057 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452638 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202058 !Sample_title = cy75_1B_CD45pos_S19 !Sample_geo_accession = GSM3202058 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452637 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202059 !Sample_title = cy75_1B_CD45pos_S26 !Sample_geo_accession = GSM3202059 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452745 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202060 !Sample_title = cy105_CD45neg_S200 !Sample_geo_accession = GSM3202060 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452744 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202061 !Sample_title = cy75_1B_CORE_CD45neg_S177 !Sample_geo_accession = GSM3202061 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452743 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202062 !Sample_title = cy75_1B_CD45pos_S70 !Sample_geo_accession = GSM3202062 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452742 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202063 !Sample_title = cy105_CD45neg_S237 !Sample_geo_accession = GSM3202063 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452741 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202064 !Sample_title = cy75_1B_CORE_CD45neg_S156 !Sample_geo_accession = GSM3202064 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452740 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202065 !Sample_title = cy75_1B_CD45pos_S81 !Sample_geo_accession = GSM3202065 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452739 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202066 !Sample_title = cy75_1B_CD45pos_S43 !Sample_geo_accession = GSM3202066 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452738 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202067 !Sample_title = cy106_CD45neg_S328 !Sample_geo_accession = GSM3202067 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452737 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202068 !Sample_title = cy105_CD45neg_S259 !Sample_geo_accession = GSM3202068 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452736 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202069 !Sample_title = cy106_CD45neg_S343 !Sample_geo_accession = GSM3202069 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452735 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202070 !Sample_title = cy75_1B_CD45pos_S21 !Sample_geo_accession = GSM3202070 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452734 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202071 !Sample_title = cy106_CD45neg_S317 !Sample_geo_accession = GSM3202071 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452733 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202072 !Sample_title = cy75_1B_CORE_CD45neg_S167 !Sample_geo_accession = GSM3202072 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452732 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202073 !Sample_title = cy75_1B_CORE_CD45neg_S103 !Sample_geo_accession = GSM3202073 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452731 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202074 !Sample_title = cy75_1B_CORE_CD45neg_S113 !Sample_geo_accession = GSM3202074 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452730 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202075 !Sample_title = cy106_CD45neg_S375 !Sample_geo_accession = GSM3202075 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452729 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202076 !Sample_title = cy106_CD45neg_S291 !Sample_geo_accession = GSM3202076 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452728 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202077 !Sample_title = cy75_1B_CD45pos_S72 !Sample_geo_accession = GSM3202077 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452727 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202078 !Sample_title = cy75_1B_CD45pos_S91 !Sample_geo_accession = GSM3202078 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452726 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202079 !Sample_title = cy106_CD45neg_S303 !Sample_geo_accession = GSM3202079 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452725 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202080 !Sample_title = cy75_1B_CD45pos_S6 !Sample_geo_accession = GSM3202080 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452724 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202081 !Sample_title = cy75_1B_CD45pos_S61 !Sample_geo_accession = GSM3202081 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452723 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202082 !Sample_title = cy75_1B_CORE_CD45neg_S109 !Sample_geo_accession = GSM3202082 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452722 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202083 !Sample_title = cy106_CD45neg_S315 !Sample_geo_accession = GSM3202083 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452721 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202084 !Sample_title = cy75_1B_CORE_CD45neg_S110 !Sample_geo_accession = GSM3202084 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452720 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202085 !Sample_title = cy105_CD45neg_S278 !Sample_geo_accession = GSM3202085 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452719 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202086 !Sample_title = cy75_1B_CD45pos_S23 !Sample_geo_accession = GSM3202086 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452784 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202087 !Sample_title = cy75_1B_CD45pos_S20 !Sample_geo_accession = GSM3202087 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452783 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202088 !Sample_title = cy106_CD45neg_S347 !Sample_geo_accession = GSM3202088 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452782 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202089 !Sample_title = cy75_1B_CD45pos_S45 !Sample_geo_accession = GSM3202089 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452827 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202090 !Sample_title = cy105_CD45neg_S219 !Sample_geo_accession = GSM3202090 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452826 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202091 !Sample_title = cy75_1B_CORE_CD45neg_S142 !Sample_geo_accession = GSM3202091 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452825 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202092 !Sample_title = cy106_CD45neg_S356 !Sample_geo_accession = GSM3202092 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452824 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202093 !Sample_title = cy105_CD45neg_S251 !Sample_geo_accession = GSM3202093 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452823 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202094 !Sample_title = cy75_1B_CD45pos_S30 !Sample_geo_accession = GSM3202094 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452822 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202095 !Sample_title = cy106_CD45neg_S353 !Sample_geo_accession = GSM3202095 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452821 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202096 !Sample_title = cy75_1B_CD45pos_S57 !Sample_geo_accession = GSM3202096 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452820 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202097 !Sample_title = cy106_CD45neg_S296 !Sample_geo_accession = GSM3202097 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452891 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202098 !Sample_title = cy105_CD45neg_S198 !Sample_geo_accession = GSM3202098 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452890 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202099 !Sample_title = cy75_1B_CD45pos_S41 !Sample_geo_accession = GSM3202099 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452889 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202100 !Sample_title = cy106_CD45neg_S320 !Sample_geo_accession = GSM3202100 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452888 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202101 !Sample_title = cy106_CD45neg_S369 !Sample_geo_accession = GSM3202101 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452887 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202102 !Sample_title = cy75_1B_CD45pos_S69 !Sample_geo_accession = GSM3202102 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452881 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202103 !Sample_title = cy75_1B_CD45pos_S78 !Sample_geo_accession = GSM3202103 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452880 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202104 !Sample_title = cy75_1B_CD45pos_S3 !Sample_geo_accession = GSM3202104 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452886 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202105 !Sample_title = cy75_1B_CD45pos_S59 !Sample_geo_accession = GSM3202105 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452885 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202106 !Sample_title = cy106_CD45neg_S313 !Sample_geo_accession = GSM3202106 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452884 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202107 !Sample_title = cy75_1B_CD45pos_S10 !Sample_geo_accession = GSM3202107 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452883 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202108 !Sample_title = cy75_1B_CD45pos_S1 !Sample_geo_accession = GSM3202108 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452882 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202109 !Sample_title = cy106_CD45neg_S380 !Sample_geo_accession = GSM3202109 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452879 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202110 !Sample_title = cy75_1B_CD45pos_S88 !Sample_geo_accession = GSM3202110 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452878 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202111 !Sample_title = cy105_CD45neg_S268 !Sample_geo_accession = GSM3202111 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452877 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202112 !Sample_title = cy75_1B_CD45pos_S83 !Sample_geo_accession = GSM3202112 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452876 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202113 !Sample_title = cy106_CD45neg_S373 !Sample_geo_accession = GSM3202113 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452875 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202114 !Sample_title = cy105_CD45neg_S276 !Sample_geo_accession = GSM3202114 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452874 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202115 !Sample_title = cy75_1B_CD45pos_S52 !Sample_geo_accession = GSM3202115 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452873 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202116 !Sample_title = cy75_1B_CORE_CD45neg_S135 !Sample_geo_accession = GSM3202116 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452872 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202117 !Sample_title = cy75_1B_CORE_CD45neg_S130 !Sample_geo_accession = GSM3202117 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452871 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202118 !Sample_title = cy75_1B_CD45pos_S16 !Sample_geo_accession = GSM3202118 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452870 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202119 !Sample_title = cy75_1B_CD45pos_S65 !Sample_geo_accession = GSM3202119 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452869 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202120 !Sample_title = cy75_1B_CD45pos_S84 !Sample_geo_accession = GSM3202120 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452868 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202121 !Sample_title = cy106_CD45neg_S381 !Sample_geo_accession = GSM3202121 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452867 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202122 !Sample_title = cy105_CD45neg_S265 !Sample_geo_accession = GSM3202122 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452866 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202123 !Sample_title = cy106_CD45neg_S377 !Sample_geo_accession = GSM3202123 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452865 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202124 !Sample_title = cy75_1B_CD45pos_S92 !Sample_geo_accession = GSM3202124 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452864 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202125 !Sample_title = cy75_1B_CD45pos_S44 !Sample_geo_accession = GSM3202125 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452863 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202126 !Sample_title = cy75_1B_CD45pos_S67 !Sample_geo_accession = GSM3202126 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452862 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202127 !Sample_title = cy105_CD45neg_S273 !Sample_geo_accession = GSM3202127 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452861 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202128 !Sample_title = cy75_1B_CORE_CD45neg_S171 !Sample_geo_accession = GSM3202128 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452860 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202129 !Sample_title = cy106_CD45neg_S372 !Sample_geo_accession = GSM3202129 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452859 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202130 !Sample_title = cy75_1B_CD45pos_S39 !Sample_geo_accession = GSM3202130 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452858 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202131 !Sample_title = cy75_1B_CORE_CD45neg_S104 !Sample_geo_accession = GSM3202131 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452857 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202132 !Sample_title = cy105_CD45neg_S249 !Sample_geo_accession = GSM3202132 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452856 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202133 !Sample_title = cy106_CD45neg_S331 !Sample_geo_accession = GSM3202133 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452855 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202134 !Sample_title = cy75_1B_CD45pos_S82 !Sample_geo_accession = GSM3202134 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452921 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202135 !Sample_title = cy75_1B_CD45pos_S86 !Sample_geo_accession = GSM3202135 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452920 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202136 !Sample_title = cy75_1B_CORE_CD45neg_S164 !Sample_geo_accession = GSM3202136 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452919 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202137 !Sample_title = cy75_1B_CORE_CD45neg_S184 !Sample_geo_accession = GSM3202137 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452918 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202138 !Sample_title = cy75_1B_CD45pos_S74 !Sample_geo_accession = GSM3202138 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452917 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202139 !Sample_title = cy75_1B_CORE_CD45neg_S152 !Sample_geo_accession = GSM3202139 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452916 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202140 !Sample_title = cy106_CD45neg_S304 !Sample_geo_accession = GSM3202140 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452915 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202141 !Sample_title = cy75_1B_CORE_CD45neg_S170 !Sample_geo_accession = GSM3202141 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452914 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202142 !Sample_title = cy75_1B_CORE_CD45neg_S151 !Sample_geo_accession = GSM3202142 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452913 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202143 !Sample_title = cy75_1B_CORE_CD45neg_S168 !Sample_geo_accession = GSM3202143 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452912 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202144 !Sample_title = cy106_CD45neg_S345 !Sample_geo_accession = GSM3202144 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452911 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202145 !Sample_title = cy75_1B_CD45pos_S28 !Sample_geo_accession = GSM3202145 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452910 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202146 !Sample_title = cy75_1B_CD45pos_S40 !Sample_geo_accession = GSM3202146 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452909 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202147 !Sample_title = cy75_1B_CORE_CD45neg_S163 !Sample_geo_accession = GSM3202147 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452908 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202148 !Sample_title = cy75_1B_CD45pos_S73 !Sample_geo_accession = GSM3202148 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452907 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202149 !Sample_title = cy105_CD45neg_S228 !Sample_geo_accession = GSM3202149 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452636 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202150 !Sample_title = cy75_1B_CORE_CD45neg_S139 !Sample_geo_accession = GSM3202150 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452635 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202151 !Sample_title = cy75_1B_CORE_CD45neg_S100 !Sample_geo_accession = GSM3202151 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452634 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202152 !Sample_title = cy106_CD45neg_S289 !Sample_geo_accession = GSM3202152 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452633 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202153 !Sample_title = cy75_1B_CD45pos_S42 !Sample_geo_accession = GSM3202153 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452632 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202154 !Sample_title = cy75_1B_CD45pos_S53 !Sample_geo_accession = GSM3202154 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452631 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202155 !Sample_title = cy75_1B_CORE_CD45neg_S149 !Sample_geo_accession = GSM3202155 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452630 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202156 !Sample_title = cy105_CD45neg_S215 !Sample_geo_accession = GSM3202156 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452629 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202157 !Sample_title = cy75_1B_CORE_CD45neg_S132 !Sample_geo_accession = GSM3202157 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452628 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202158 !Sample_title = cy106_CD45neg_S357 !Sample_geo_accession = GSM3202158 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452627 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202159 !Sample_title = cy75_1B_CD45pos_S29 !Sample_geo_accession = GSM3202159 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452626 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202160 !Sample_title = cy75_1B_CD45pos_S15 !Sample_geo_accession = GSM3202160 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452625 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202161 !Sample_title = cy75_1B_CD45pos_S75 !Sample_geo_accession = GSM3202161 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452624 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202162 !Sample_title = cy75_1B_CD45pos_S5 !Sample_geo_accession = GSM3202162 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452623 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202163 !Sample_title = cy75_1B_CORE_CD45neg_S124 !Sample_geo_accession = GSM3202163 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452622 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202164 !Sample_title = cy75_1B_CD45pos_S54 !Sample_geo_accession = GSM3202164 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452621 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202165 !Sample_title = cy75_1B_CORE_CD45neg_S108 !Sample_geo_accession = GSM3202165 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452620 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202166 !Sample_title = cy75_1B_CD45pos_S76 !Sample_geo_accession = GSM3202166 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452619 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202167 !Sample_title = cy105_CD45neg_S202 !Sample_geo_accession = GSM3202167 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452618 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202168 !Sample_title = cy75_1B_CORE_CD45neg_S107 !Sample_geo_accession = GSM3202168 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452617 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202169 !Sample_title = cy106_CD45neg_S290 !Sample_geo_accession = GSM3202169 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452616 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202170 !Sample_title = cy75_1B_CD45pos_S49 !Sample_geo_accession = GSM3202170 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452615 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202171 !Sample_title = cy75_1B_CD45pos_S11 !Sample_geo_accession = GSM3202171 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452614 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202172 !Sample_title = cy75_1B_CD45pos_S50 !Sample_geo_accession = GSM3202172 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452613 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202173 !Sample_title = cy75_1B_CORE_CD45neg_S119 !Sample_geo_accession = GSM3202173 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452612 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202174 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S191 !Sample_geo_accession = GSM3202174 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452611 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202175 !Sample_title = cy116_CD45pos_S343 !Sample_geo_accession = GSM3202175 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452610 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202176 !Sample_title = cy116_CD45pos_S311 !Sample_geo_accession = GSM3202176 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452609 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202177 !Sample_title = cy116_CD45pos_S312 !Sample_geo_accession = GSM3202177 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452608 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202178 !Sample_title = cy98_CD45neg_CD90neg_S44 !Sample_geo_accession = GSM3202178 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452607 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202179 !Sample_title = cy98_CD45neg_CD90neg_S14 !Sample_geo_accession = GSM3202179 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452817 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202180 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S144 !Sample_geo_accession = GSM3202180 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452816 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202181 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S188 !Sample_geo_accession = GSM3202181 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452815 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202182 !Sample_title = cy98_CD45neg_CD90neg_S46 !Sample_geo_accession = GSM3202182 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452814 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202183 !Sample_title = cy98_CD45neg_CD90neg_S32 !Sample_geo_accession = GSM3202183 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452813 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202184 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S104 !Sample_geo_accession = GSM3202184 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452812 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202185 !Sample_title = cy98_CD45neg_CD90neg_S35 !Sample_geo_accession = GSM3202185 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452811 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202186 !Sample_title = cy98_CD45neg_CD90neg_S33 !Sample_geo_accession = GSM3202186 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452810 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202187 !Sample_title = cy98_CD45neg_CD90neg_S96 !Sample_geo_accession = GSM3202187 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452809 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202188 !Sample_title = cy98_CD45neg_CD90neg_S3 !Sample_geo_accession = GSM3202188 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452808 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202189 !Sample_title = cy98_CD45neg_CD90neg_S78 !Sample_geo_accession = GSM3202189 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452807 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202190 !Sample_title = cy98_CD45neg_CD90neg_S70 !Sample_geo_accession = GSM3202190 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452806 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202191 !Sample_title = cy98_CD45neg_CD90neg_S48 !Sample_geo_accession = GSM3202191 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452805 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202192 !Sample_title = cy116_CD45pos_S346 !Sample_geo_accession = GSM3202192 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452804 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202193 !Sample_title = cy116_CD45pos_S289 !Sample_geo_accession = GSM3202193 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452797 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202194 !Sample_title = cy98_CD45neg_CD90neg_S72 !Sample_geo_accession = GSM3202194 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452803 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202195 !Sample_title = cy98_CD45neg_CD90neg_S56 !Sample_geo_accession = GSM3202195 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452802 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202196 !Sample_title = cy116_CD45pos_S349 !Sample_geo_accession = GSM3202196 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452801 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202197 !Sample_title = cy98_CD45neg_CD90neg_S71 !Sample_geo_accession = GSM3202197 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452800 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202198 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S181 !Sample_geo_accession = GSM3202198 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452799 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202199 !Sample_title = cy98_CD45neg_CD90neg_S84 !Sample_geo_accession = GSM3202199 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452798 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202200 !Sample_title = cy98_CD45neg_CD90neg_S30 !Sample_geo_accession = GSM3202200 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452796 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202201 !Sample_title = cy98_CD45neg_CD90neg_S23 !Sample_geo_accession = GSM3202201 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452795 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202202 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S124 !Sample_geo_accession = GSM3202202 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452794 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202203 !Sample_title = cy116_CD45pos_S361 !Sample_geo_accession = GSM3202203 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452793 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202204 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S143 !Sample_geo_accession = GSM3202204 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452792 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202205 !Sample_title = cy98_CD45neg_CD90neg_S5 !Sample_geo_accession = GSM3202205 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452791 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202206 !Sample_title = cy116_CD45pos_S341 !Sample_geo_accession = GSM3202206 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452790 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202207 !Sample_title = cy98_CD45neg_CD90neg_S6 !Sample_geo_accession = GSM3202207 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452789 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202208 !Sample_title = cy98_CD45neg_CD90neg_S49 !Sample_geo_accession = GSM3202208 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452788 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202209 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S148 !Sample_geo_accession = GSM3202209 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452781 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202210 !Sample_title = cy116_CD45pos_S344 !Sample_geo_accession = GSM3202210 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452780 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202211 !Sample_title = cy116_CD45pos_S315 !Sample_geo_accession = GSM3202211 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452779 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202212 !Sample_title = cy116_CD45pos_S326 !Sample_geo_accession = GSM3202212 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452778 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202213 !Sample_title = cy116_CD45pos_S338 !Sample_geo_accession = GSM3202213 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452777 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202214 !Sample_title = cy98_CD45neg_CD90neg_S74 !Sample_geo_accession = GSM3202214 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452776 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202215 !Sample_title = cy98_CD45neg_CD90neg_S13 !Sample_geo_accession = GSM3202215 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452775 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202216 !Sample_title = cy98_CD45neg_CD90neg_S19 !Sample_geo_accession = GSM3202216 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452774 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202217 !Sample_title = cy116_CD45pos_S308 !Sample_geo_accession = GSM3202217 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452773 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202218 !Sample_title = cy116_CD45pos_S364 !Sample_geo_accession = GSM3202218 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452772 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202219 !Sample_title = cy98_CD45neg_CD90neg_S31 !Sample_geo_accession = GSM3202219 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452771 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202220 !Sample_title = cy98_CD45neg_CD90neg_S61 !Sample_geo_accession = GSM3202220 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452770 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202221 !Sample_title = cy98_CD45neg_CD90neg_S76 !Sample_geo_accession = GSM3202221 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452769 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202222 !Sample_title = cy116_CD45pos_S367 !Sample_geo_accession = GSM3202222 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452768 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202223 !Sample_title = cy98_CD45neg_CD90neg_S80 !Sample_geo_accession = GSM3202223 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452767 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202224 !Sample_title = cy98_CD45neg_CD90neg_S8 !Sample_geo_accession = GSM3202224 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452766 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202225 !Sample_title = cy98_CD45neg_CD90neg_S93 !Sample_geo_accession = GSM3202225 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452765 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202226 !Sample_title = cy116_CD45pos_S336 !Sample_geo_accession = GSM3202226 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452764 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202227 !Sample_title = cy98_CD45neg_CD90neg_S15 !Sample_geo_accession = GSM3202227 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452763 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202228 !Sample_title = cy116_CD45pos_S345 !Sample_geo_accession = GSM3202228 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452762 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202229 !Sample_title = cy116_CD45pos_S339 !Sample_geo_accession = GSM3202229 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452761 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202230 !Sample_title = cy116_CD45pos_S365 !Sample_geo_accession = GSM3202230 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452760 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202231 !Sample_title = cy116_CD45pos_S360 !Sample_geo_accession = GSM3202231 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452759 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202232 !Sample_title = cy98_CD45neg_CD90neg_S39 !Sample_geo_accession = GSM3202232 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452758 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202233 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S137 !Sample_geo_accession = GSM3202233 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452757 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202234 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S128 !Sample_geo_accession = GSM3202234 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452756 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202235 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S109 !Sample_geo_accession = GSM3202235 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452755 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202236 !Sample_title = cy98_CD45neg_CD90neg_S45 !Sample_geo_accession = GSM3202236 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452754 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202237 !Sample_title = cy98_CD45neg_CD90neg_S34 !Sample_geo_accession = GSM3202237 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452819 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202238 !Sample_title = cy98_CD45neg_CD90neg_S43 !Sample_geo_accession = GSM3202238 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452818 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202239 !Sample_title = cy116_CD45pos_S350 !Sample_geo_accession = GSM3202239 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450389 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202240 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S130 !Sample_geo_accession = GSM3202240 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450388 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202241 !Sample_title = cy116_CD45pos_S358 !Sample_geo_accession = GSM3202241 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450387 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202242 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S103 !Sample_geo_accession = GSM3202242 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450386 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202243 !Sample_title = cy116_CD45pos_S324 !Sample_geo_accession = GSM3202243 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450385 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202244 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S169 !Sample_geo_accession = GSM3202244 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450384 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202245 !Sample_title = cy116_CD45pos_S329 !Sample_geo_accession = GSM3202245 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450383 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202246 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S102 !Sample_geo_accession = GSM3202246 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450382 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202247 !Sample_title = cy98_CD45neg_CD90neg_S54 !Sample_geo_accession = GSM3202247 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450381 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202248 !Sample_title = cy98_CD45neg_CD90neg_S28 !Sample_geo_accession = GSM3202248 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450380 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202249 !Sample_title = cy98_CD45neg_CD90neg_S10 !Sample_geo_accession = GSM3202249 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450452 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202250 !Sample_title = cy116_CD45pos_S371 !Sample_geo_accession = GSM3202250 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450451 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202251 !Sample_title = cy98_CD45neg_CD90neg_S69 !Sample_geo_accession = GSM3202251 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450449 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202252 !Sample_title = cy116_CD45pos_S376 !Sample_geo_accession = GSM3202252 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450448 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202253 !Sample_title = cy116_CD45pos_S374 !Sample_geo_accession = GSM3202253 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450447 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202254 !Sample_title = cy98_CD45neg_CD90neg_S67 !Sample_geo_accession = GSM3202254 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450379 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202255 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S139 !Sample_geo_accession = GSM3202255 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450453 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202256 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S147 !Sample_geo_accession = GSM3202256 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450446 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202257 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S141 !Sample_geo_accession = GSM3202257 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450445 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202258 !Sample_title = cy116_CD45pos_S380 !Sample_geo_accession = GSM3202258 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450444 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202259 !Sample_title = cy116_CD45pos_S362 !Sample_geo_accession = GSM3202259 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450443 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202260 !Sample_title = cy116_CD45pos_S309 !Sample_geo_accession = GSM3202260 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450442 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202261 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S105 !Sample_geo_accession = GSM3202261 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450441 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202262 !Sample_title = cy116_CD45pos_S313 !Sample_geo_accession = GSM3202262 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450440 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202263 !Sample_title = cy98_CD45neg_CD90neg_S77 !Sample_geo_accession = GSM3202263 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450439 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202264 !Sample_title = cy98_CD45neg_CD90neg_S41 !Sample_geo_accession = GSM3202264 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450438 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202265 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S185 !Sample_geo_accession = GSM3202265 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450437 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202266 !Sample_title = cy98_CD45neg_CD90neg_S55 !Sample_geo_accession = GSM3202266 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450436 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202267 !Sample_title = cy116_CD45pos_S354 !Sample_geo_accession = GSM3202267 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450435 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202268 !Sample_title = cy116_CD45pos_S323 !Sample_geo_accession = GSM3202268 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450434 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202269 !Sample_title = cy116_CD45pos_S378 !Sample_geo_accession = GSM3202269 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450719 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202270 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S158 !Sample_geo_accession = GSM3202270 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450718 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202271 !Sample_title = cy116_CD45pos_S322 !Sample_geo_accession = GSM3202271 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450717 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202272 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S97 !Sample_geo_accession = GSM3202272 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450716 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202273 !Sample_title = cy116_CD45pos_S290 !Sample_geo_accession = GSM3202273 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450715 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202274 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S192 !Sample_geo_accession = GSM3202274 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450714 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202275 !Sample_title = cy116_CD45pos_S318 !Sample_geo_accession = GSM3202275 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450713 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202276 !Sample_title = cy98_CD45neg_CD90neg_S38 !Sample_geo_accession = GSM3202276 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450712 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202277 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S107 !Sample_geo_accession = GSM3202277 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450711 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202278 !Sample_title = cy116_CD45pos_S292 !Sample_geo_accession = GSM3202278 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450710 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202279 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S183 !Sample_geo_accession = GSM3202279 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450778 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202280 !Sample_title = cy116_CD45pos_S327 !Sample_geo_accession = GSM3202280 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450777 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202281 !Sample_title = cy98_CD45neg_CD90neg_S68 !Sample_geo_accession = GSM3202281 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450776 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202282 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S171 !Sample_geo_accession = GSM3202282 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450770 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202283 !Sample_title = cy116_CD45pos_S305 !Sample_geo_accession = GSM3202283 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450769 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202284 !Sample_title = cy116_CD45pos_S340 !Sample_geo_accession = GSM3202284 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450775 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202285 !Sample_title = cy116_CD45pos_S333 !Sample_geo_accession = GSM3202285 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450774 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202286 !Sample_title = cy116_CD45pos_S306 !Sample_geo_accession = GSM3202286 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450773 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202287 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S157 !Sample_geo_accession = GSM3202287 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450772 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202288 !Sample_title = cy116_CD45pos_S347 !Sample_geo_accession = GSM3202288 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450771 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202289 !Sample_title = cy98_CD45neg_CD90neg_S37 !Sample_geo_accession = GSM3202289 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450768 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202290 !Sample_title = cy116_CD45pos_S296 !Sample_geo_accession = GSM3202290 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450767 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202291 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S180 !Sample_geo_accession = GSM3202291 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450766 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202292 !Sample_title = cy98_CD45neg_CD90neg_S83 !Sample_geo_accession = GSM3202292 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450765 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202293 !Sample_title = cy116_CD45pos_S359 !Sample_geo_accession = GSM3202293 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450764 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202294 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S101 !Sample_geo_accession = GSM3202294 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450762 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202295 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S142 !Sample_geo_accession = GSM3202295 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450761 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202296 !Sample_title = cy116_CD45pos_S357 !Sample_geo_accession = GSM3202296 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450760 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202297 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S123 !Sample_geo_accession = GSM3202297 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450759 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202298 !Sample_title = cy98_CD45neg_CD90neg_S53 !Sample_geo_accession = GSM3202298 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450758 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202299 !Sample_title = cy98_CD45neg_CD90neg_S58 !Sample_geo_accession = GSM3202299 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450525 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202300 !Sample_title = cy116_CD45pos_S303 !Sample_geo_accession = GSM3202300 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450524 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202301 !Sample_title = cy98_CD45neg_CD90neg_S64 !Sample_geo_accession = GSM3202301 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450523 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202302 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S121 !Sample_geo_accession = GSM3202302 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450522 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202303 !Sample_title = cy116_CD45pos_S363 !Sample_geo_accession = GSM3202303 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450521 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202304 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S140 !Sample_geo_accession = GSM3202304 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450520 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202305 !Sample_title = cy98_CD45neg_CD90neg_S91 !Sample_geo_accession = GSM3202305 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450519 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202306 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S150 !Sample_geo_accession = GSM3202306 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450518 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202307 !Sample_title = cy98_CD45neg_CD90neg_S86 !Sample_geo_accession = GSM3202307 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450517 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202308 !Sample_title = cy116_CD45pos_S332 !Sample_geo_accession = GSM3202308 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450515 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202309 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S182 !Sample_geo_accession = GSM3202309 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450514 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202310 !Sample_title = cy116_CD45neg_CD90neg_S244 !Sample_geo_accession = GSM3202310 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450513 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202311 !Sample_title = cy116_CD45pos_S293 !Sample_geo_accession = GSM3202311 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450512 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202312 !Sample_title = cy98_CD45neg_CD90neg_S62 !Sample_geo_accession = GSM3202312 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450511 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202313 !Sample_title = cy116_CD45pos_S379 !Sample_geo_accession = GSM3202313 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450510 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202314 !Sample_title = cy116_CD45pos_S301 !Sample_geo_accession = GSM3202314 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450516 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202315 !Sample_title = cy116_CD45pos_S297 !Sample_geo_accession = GSM3202315 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450509 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202316 !Sample_title = cy116_CD45pos_S334 !Sample_geo_accession = GSM3202316 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450508 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202317 !Sample_title = cy98_CD45neg_CD90neg_S92 !Sample_geo_accession = GSM3202317 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450507 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202318 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S122 !Sample_geo_accession = GSM3202318 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450506 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202319 !Sample_title = cy98_CD45neg_CD90neg_S24 !Sample_geo_accession = GSM3202319 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450505 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202320 !Sample_title = cy98_CD45neg_CD90neg_S2 !Sample_geo_accession = GSM3202320 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450504 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202321 !Sample_title = cy116_CD45pos_S351 !Sample_geo_accession = GSM3202321 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450503 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202322 !Sample_title = cy116_CD45pos_S307 !Sample_geo_accession = GSM3202322 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450502 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202323 !Sample_title = cy98_CD45neg_CD90neg_S94 !Sample_geo_accession = GSM3202323 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450501 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202324 !Sample_title = cy116_CD45pos_S352 !Sample_geo_accession = GSM3202324 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450500 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202325 !Sample_title = cy116_CD45pos_S331 !Sample_geo_accession = GSM3202325 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450499 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202326 !Sample_title = cy116_CD45pos_S310 !Sample_geo_accession = GSM3202326 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450498 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202327 !Sample_title = cy116_CD45pos_S294 !Sample_geo_accession = GSM3202327 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450497 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202328 !Sample_title = cy116_CD45pos_S302 !Sample_geo_accession = GSM3202328 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450496 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202329 !Sample_title = cy98_CD45neg_CD90neg_S60 !Sample_geo_accession = GSM3202329 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450495 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202330 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S116 !Sample_geo_accession = GSM3202330 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450494 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202331 !Sample_title = cy98_CD45neg_CD90neg_S26 !Sample_geo_accession = GSM3202331 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450493 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202332 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S145 !Sample_geo_accession = GSM3202332 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450492 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202333 !Sample_title = cy116_CD45pos_S300 !Sample_geo_accession = GSM3202333 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450491 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202334 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S119 !Sample_geo_accession = GSM3202334 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450490 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202335 !Sample_title = cy116_CD45pos_S321 !Sample_geo_accession = GSM3202335 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450489 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202336 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S111 !Sample_geo_accession = GSM3202336 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450562 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202337 !Sample_title = cy98_CD45neg_CD90neg_S11 !Sample_geo_accession = GSM3202337 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450561 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202338 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S166 !Sample_geo_accession = GSM3202338 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450560 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202339 !Sample_title = cy116_CD45pos_S356 !Sample_geo_accession = GSM3202339 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450559 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202340 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S132 !Sample_geo_accession = GSM3202340 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450558 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202341 !Sample_title = cy98_CD45neg_CD90neg_S36 !Sample_geo_accession = GSM3202341 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450557 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202342 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S127 !Sample_geo_accession = GSM3202342 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450552 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202343 !Sample_title = cy116_CD45pos_S304 !Sample_geo_accession = GSM3202343 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450551 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202344 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S172 !Sample_geo_accession = GSM3202344 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450556 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202345 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S170 !Sample_geo_accession = GSM3202345 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450555 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202346 !Sample_title = cy98_CD45neg_CD90neg_S25 !Sample_geo_accession = GSM3202346 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450554 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202347 !Sample_title = cy116_CD45pos_S295 !Sample_geo_accession = GSM3202347 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450553 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202348 !Sample_title = cy116_CD45pos_S366 !Sample_geo_accession = GSM3202348 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450550 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202349 !Sample_title = cy116_CD45pos_S372 !Sample_geo_accession = GSM3202349 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450549 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202350 !Sample_title = cy116_CD45pos_S291 !Sample_geo_accession = GSM3202350 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450548 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202351 !Sample_title = cy98_CD45neg_CD90neg_S40 !Sample_geo_accession = GSM3202351 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450547 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202352 !Sample_title = cy98_CD45neg_CD90neg_S75 !Sample_geo_accession = GSM3202352 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450546 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202353 !Sample_title = cy116_CD45pos_S298 !Sample_geo_accession = GSM3202353 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450545 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202354 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S151 !Sample_geo_accession = GSM3202354 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450544 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202355 !Sample_title = cy116_CD45pos_S370 !Sample_geo_accession = GSM3202355 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450543 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202356 !Sample_title = cy98_CD45neg_CD90neg_S66 !Sample_geo_accession = GSM3202356 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450542 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202357 !Sample_title = cy98_CD45neg_CD90neg_S18 !Sample_geo_accession = GSM3202357 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450540 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202358 !Sample_title = cy98_CD45neg_CD90neg_S50 !Sample_geo_accession = GSM3202358 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450539 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202359 !Sample_title = cy98_CD45neg_CD90neg_S29 !Sample_geo_accession = GSM3202359 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450677 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202360 !Sample_title = cy116_CD45pos_S316 !Sample_geo_accession = GSM3202360 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450676 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202361 !Sample_title = cy98_CD45neg_CD90neg_S88 !Sample_geo_accession = GSM3202361 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450675 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202362 !Sample_title = cy116_CD45pos_S373 !Sample_geo_accession = GSM3202362 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450674 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202363 !Sample_title = cy98_CD45neg_CD90neg_S57 !Sample_geo_accession = GSM3202363 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450673 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202364 !Sample_title = cy116_CD45pos_S320 !Sample_geo_accession = GSM3202364 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450744 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202365 !Sample_title = cy98_CD45neg_CD90neg_S22 !Sample_geo_accession = GSM3202365 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450743 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202366 !Sample_title = cy98_CD45neg_CD90neg_S51 !Sample_geo_accession = GSM3202366 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450742 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202367 !Sample_title = cy116_CD45pos_S382 !Sample_geo_accession = GSM3202367 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450741 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202368 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S134 !Sample_geo_accession = GSM3202368 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450739 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202369 !Sample_title = cy116_CD45pos_S355 !Sample_geo_accession = GSM3202369 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450738 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202370 !Sample_title = cy116_CD45pos_S377 !Sample_geo_accession = GSM3202370 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450737 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202371 !Sample_title = cy98_CD45neg_CD90neg_S90 !Sample_geo_accession = GSM3202371 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450736 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202372 !Sample_title = cy98_CD45neg_CD90neg_S27 !Sample_geo_accession = GSM3202372 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450735 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202373 !Sample_title = cy98_CD45neg_CD90neg_S16 !Sample_geo_accession = GSM3202373 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450734 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202374 !Sample_title = cy105_Zombineg_CD45neg_CD90neg_S100 !Sample_geo_accession = GSM3202374 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450740 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202375 !Sample_title = cy98_CD45neg_CD90neg_S4 !Sample_geo_accession = GSM3202375 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450733 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202376 !Sample_title = cy98_CD45neg_CD90neg_S7 !Sample_geo_accession = GSM3202376 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450732 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202377 !Sample_title = cy116_CD45pos_S319 !Sample_geo_accession = GSM3202377 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450731 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202378 !Sample_title = cy116_CD45pos_S325 !Sample_geo_accession = GSM3202378 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450730 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202379 !Sample_title = cy116_CD45pos_S383 !Sample_geo_accession = GSM3202379 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450729 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202380 !Sample_title = cy98_CD45neg_CD90neg_S1 !Sample_geo_accession = GSM3202380 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450728 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202381 !Sample_title = cy112_CD45neg_CD90neg_S120 !Sample_geo_accession = GSM3202381 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450727 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202382 !Sample_title = cy102_CD45neg_CD90neg_S340 !Sample_geo_accession = GSM3202382 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450726 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202383 !Sample_title = cy102_CD45neg_CD90neg_S376 !Sample_geo_accession = GSM3202383 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450725 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202384 !Sample_title = cy112_CD45pos_S9 !Sample_geo_accession = GSM3202384 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450724 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202385 !Sample_title = cy102_CD45neg_CD90neg_S290 !Sample_geo_accession = GSM3202385 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450723 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202386 !Sample_title = cy102_CD45pos_S196 !Sample_geo_accession = GSM3202386 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450722 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202387 !Sample_title = cy102_CD45pos_S211 !Sample_geo_accession = GSM3202387 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450721 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202388 !Sample_title = cy102_CD45pos_S272 !Sample_geo_accession = GSM3202388 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450720 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202389 !Sample_title = cy102_CD45pos_S274 !Sample_geo_accession = GSM3202389 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451106 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202390 !Sample_title = cy102_CD45neg_CD90neg_S331 !Sample_geo_accession = GSM3202390 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451105 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202391 !Sample_title = cy112_CD45pos_S61 !Sample_geo_accession = GSM3202391 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451104 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202392 !Sample_title = cy112_CD45pos_S7 !Sample_geo_accession = GSM3202392 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451103 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202393 !Sample_title = cy112_CD45neg_CD90neg_S181 !Sample_geo_accession = GSM3202393 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451102 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202394 !Sample_title = cy102_CD45pos_S233 !Sample_geo_accession = GSM3202394 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451101 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202395 !Sample_title = cy102_CD45neg_CD90neg_S318 !Sample_geo_accession = GSM3202395 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451100 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202396 !Sample_title = cy102_CD45neg_CD90neg_S360 !Sample_geo_accession = GSM3202396 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451099 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202397 !Sample_title = cy112_CD45pos_S41 !Sample_geo_accession = GSM3202397 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451098 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202398 !Sample_title = cy102_CD45neg_CD90neg_S316 !Sample_geo_accession = GSM3202398 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451097 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202399 !Sample_title = cy102_CD45neg_CD90neg_S372 !Sample_geo_accession = GSM3202399 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451096 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202400 !Sample_title = cy102_CD45neg_CD90neg_S298 !Sample_geo_accession = GSM3202400 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451095 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202401 !Sample_title = cy102_CD45pos_S249 !Sample_geo_accession = GSM3202401 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451092 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202402 !Sample_title = cy102_CD45pos_S235 !Sample_geo_accession = GSM3202402 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451091 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202403 !Sample_title = cy112_CD45pos_S88 !Sample_geo_accession = GSM3202403 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451090 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202404 !Sample_title = cy112_CD45pos_S27 !Sample_geo_accession = GSM3202404 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451094 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202405 !Sample_title = cy102_CD45neg_CD90neg_S378 !Sample_geo_accession = GSM3202405 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451093 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202406 !Sample_title = cy102_CD45pos_S253 !Sample_geo_accession = GSM3202406 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451089 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202407 !Sample_title = cy102_CD45neg_CD90neg_S345 !Sample_geo_accession = GSM3202407 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451088 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202408 !Sample_title = cy102_CD45pos_S194 !Sample_geo_accession = GSM3202408 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451087 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202409 !Sample_title = cy102_CD45pos_S244 !Sample_geo_accession = GSM3202409 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451086 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202410 !Sample_title = cy102_CD45neg_CD90neg_S377 !Sample_geo_accession = GSM3202410 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451085 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202411 !Sample_title = cy102_CD45neg_CD90neg_S382 !Sample_geo_accession = GSM3202411 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451084 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202412 !Sample_title = cy102_CD45pos_S275 !Sample_geo_accession = GSM3202412 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451083 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202413 !Sample_title = cy112_CD45pos_S35 !Sample_geo_accession = GSM3202413 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451082 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202414 !Sample_title = cy102_CD45pos_S234 !Sample_geo_accession = GSM3202414 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451081 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202415 !Sample_title = cy102_CD45pos_S282 !Sample_geo_accession = GSM3202415 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451080 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202416 !Sample_title = cy102_CD45pos_S254 !Sample_geo_accession = GSM3202416 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451079 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202417 !Sample_title = cy102_CD45neg_CD90neg_S295 !Sample_geo_accession = GSM3202417 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451078 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202418 !Sample_title = cy112_CD45neg_CD90neg_S127 !Sample_geo_accession = GSM3202418 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451077 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202419 !Sample_title = cy102_CD45pos_S205 !Sample_geo_accession = GSM3202419 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451076 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202420 !Sample_title = cy112_CD45pos_S66 !Sample_geo_accession = GSM3202420 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451075 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202421 !Sample_title = cy102_CD45neg_CD90neg_S307 !Sample_geo_accession = GSM3202421 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451145 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202422 !Sample_title = cy102_CD45neg_CD90neg_S365 !Sample_geo_accession = GSM3202422 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451144 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202423 !Sample_title = cy102_CD45pos_S200 !Sample_geo_accession = GSM3202423 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451143 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202424 !Sample_title = cy102_CD45pos_S232 !Sample_geo_accession = GSM3202424 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451142 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202425 !Sample_title = cy102_CD45pos_S236 !Sample_geo_accession = GSM3202425 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451141 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202426 !Sample_title = cy112_CD45pos_S83 !Sample_geo_accession = GSM3202426 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451140 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202427 !Sample_title = cy102_CD45neg_CD90neg_S363 !Sample_geo_accession = GSM3202427 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451139 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202428 !Sample_title = cy102_CD45neg_CD90neg_S293 !Sample_geo_accession = GSM3202428 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451138 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202429 !Sample_title = cy102_CD45neg_CD90neg_S300 !Sample_geo_accession = GSM3202429 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451137 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202430 !Sample_title = cy102_CD45neg_CD90neg_S311 !Sample_geo_accession = GSM3202430 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451136 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202431 !Sample_title = cy102_CD45neg_CD90neg_S330 !Sample_geo_accession = GSM3202431 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451135 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202432 !Sample_title = cy102_CD45pos_S240 !Sample_geo_accession = GSM3202432 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451134 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202433 !Sample_title = cy112_CD45neg_CD90neg_S169 !Sample_geo_accession = GSM3202433 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451133 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202434 !Sample_title = cy112_CD45neg_CD90neg_S108 !Sample_geo_accession = GSM3202434 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451132 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202435 !Sample_title = cy102_CD45pos_S221 !Sample_geo_accession = GSM3202435 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451131 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202436 !Sample_title = cy102_CD45pos_S224 !Sample_geo_accession = GSM3202436 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451130 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202437 !Sample_title = cy112_CD45pos_S31 !Sample_geo_accession = GSM3202437 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451129 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202438 !Sample_title = cy112_CD45neg_CD90neg_S178 !Sample_geo_accession = GSM3202438 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451128 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202439 !Sample_title = cy102_CD45neg_CD90neg_S291 !Sample_geo_accession = GSM3202439 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451127 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202440 !Sample_title = cy102_CD45neg_CD90neg_S359 !Sample_geo_accession = GSM3202440 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451126 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202441 !Sample_title = cy112_CD45pos_S47 !Sample_geo_accession = GSM3202441 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451125 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202442 !Sample_title = cy102_CD45neg_CD90neg_S310 !Sample_geo_accession = GSM3202442 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451124 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202443 !Sample_title = cy102_CD45pos_S206 !Sample_geo_accession = GSM3202443 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451123 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202444 !Sample_title = cy112_CD45pos_S40 !Sample_geo_accession = GSM3202444 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451122 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202445 !Sample_title = cy102_CD45pos_S279 !Sample_geo_accession = GSM3202445 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451121 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202446 !Sample_title = cy102_CD45pos_S278 !Sample_geo_accession = GSM3202446 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451120 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202447 !Sample_title = cy102_CD45neg_CD90neg_S319 !Sample_geo_accession = GSM3202447 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451119 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202448 !Sample_title = cy102_CD45pos_S239 !Sample_geo_accession = GSM3202448 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451118 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202449 !Sample_title = cy112_CD45pos_S69 !Sample_geo_accession = GSM3202449 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451059 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202450 !Sample_title = cy102_CD45neg_CD90neg_S301 !Sample_geo_accession = GSM3202450 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451058 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202451 !Sample_title = cy102_CD45neg_CD90neg_S371 !Sample_geo_accession = GSM3202451 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451057 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202452 !Sample_title = cy102_CD45pos_S219 !Sample_geo_accession = GSM3202452 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451056 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202453 !Sample_title = cy102_CD45pos_S238 !Sample_geo_accession = GSM3202453 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451055 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202454 !Sample_title = cy102_CD45pos_S248 !Sample_geo_accession = GSM3202454 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451054 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202455 !Sample_title = cy102_CD45pos_S204 !Sample_geo_accession = GSM3202455 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451053 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202456 !Sample_title = cy102_CD45neg_CD90neg_S344 !Sample_geo_accession = GSM3202456 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451052 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202457 !Sample_title = cy102_CD45pos_S271 !Sample_geo_accession = GSM3202457 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451051 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202458 !Sample_title = cy102_CD45neg_CD90neg_S358 !Sample_geo_accession = GSM3202458 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451050 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202459 !Sample_title = cy112_CD45pos_S39 !Sample_geo_accession = GSM3202459 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451047 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202460 !Sample_title = cy102_CD45pos_S284 !Sample_geo_accession = GSM3202460 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451046 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202461 !Sample_title = cy102_CD45neg_CD90neg_S380 !Sample_geo_accession = GSM3202461 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451045 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202462 !Sample_title = cy102_CD45neg_CD90neg_S370 !Sample_geo_accession = GSM3202462 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451044 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202463 !Sample_title = cy112_CD45pos_S17 !Sample_geo_accession = GSM3202463 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451043 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202464 !Sample_title = cy102_CD45pos_S261 !Sample_geo_accession = GSM3202464 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451049 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202465 !Sample_title = cy102_CD45neg_CD90neg_S381 !Sample_geo_accession = GSM3202465 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451048 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202466 !Sample_title = cy102_CD45pos_S259 !Sample_geo_accession = GSM3202466 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451042 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202467 !Sample_title = cy112_CD45pos_S63 !Sample_geo_accession = GSM3202467 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451041 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202468 !Sample_title = cy102_CD45neg_CD90neg_S354 !Sample_geo_accession = GSM3202468 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451040 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202469 !Sample_title = cy112_CD45neg_CD90neg_S102 !Sample_geo_accession = GSM3202469 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451039 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202470 !Sample_title = cy102_CD45neg_CD90neg_S342 !Sample_geo_accession = GSM3202470 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451038 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202471 !Sample_title = cy102_CD45neg_CD90neg_S334 !Sample_geo_accession = GSM3202471 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451037 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202472 !Sample_title = cy112_CD45neg_CD90neg_S176 !Sample_geo_accession = GSM3202472 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451113 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202473 !Sample_title = cy102_CD45pos_S201 !Sample_geo_accession = GSM3202473 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451112 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202474 !Sample_title = cy102_CD45neg_CD90neg_S302 !Sample_geo_accession = GSM3202474 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451111 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202475 !Sample_title = cy112_CD45pos_S14 !Sample_geo_accession = GSM3202475 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451110 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202476 !Sample_title = cy102_CD45pos_S283 !Sample_geo_accession = GSM3202476 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451109 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202477 !Sample_title = cy102_CD45pos_S241 !Sample_geo_accession = GSM3202477 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451108 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202478 !Sample_title = cy102_CD45pos_S252 !Sample_geo_accession = GSM3202478 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451107 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202479 !Sample_title = cy102_CD45pos_S220 !Sample_geo_accession = GSM3202479 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451015 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202480 !Sample_title = cy102_CD45pos_S225 !Sample_geo_accession = GSM3202480 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451014 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202481 !Sample_title = cy102_CD45pos_S198 !Sample_geo_accession = GSM3202481 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451013 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202482 !Sample_title = cy102_CD45neg_CD90neg_S315 !Sample_geo_accession = GSM3202482 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451012 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202483 !Sample_title = cy102_CD45pos_S237 !Sample_geo_accession = GSM3202483 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451011 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202484 !Sample_title = cy112_CD45pos_S42 !Sample_geo_accession = GSM3202484 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451010 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202485 !Sample_title = cy102_CD45neg_CD90neg_S289 !Sample_geo_accession = GSM3202485 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451009 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202486 !Sample_title = cy102_CD45pos_S209 !Sample_geo_accession = GSM3202486 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451008 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202487 !Sample_title = cy102_CD45neg_CD90neg_S362 !Sample_geo_accession = GSM3202487 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451007 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202488 !Sample_title = cy102_CD45neg_CD90neg_S314 !Sample_geo_accession = GSM3202488 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451006 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202489 !Sample_title = cy102_CD45neg_CD90neg_S299 !Sample_geo_accession = GSM3202489 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451005 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202490 !Sample_title = cy102_CD45pos_S195 !Sample_geo_accession = GSM3202490 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451004 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202491 !Sample_title = cy102_CD45neg_CD90neg_S343 !Sample_geo_accession = GSM3202491 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451003 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202492 !Sample_title = cy102_CD45pos_S216 !Sample_geo_accession = GSM3202492 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451002 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202493 !Sample_title = cy112_CD45pos_S58 !Sample_geo_accession = GSM3202493 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451001 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202494 !Sample_title = cy102_CD45neg_CD90neg_S364 !Sample_geo_accession = GSM3202494 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451074 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202495 !Sample_title = cy102_CD45pos_S217 !Sample_geo_accession = GSM3202495 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451073 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202496 !Sample_title = cy102_CD45pos_S267 !Sample_geo_accession = GSM3202496 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451072 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202497 !Sample_title = cy112_CD45pos_S15 !Sample_geo_accession = GSM3202497 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451071 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202498 !Sample_title = cy102_CD45pos_S250 !Sample_geo_accession = GSM3202498 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451070 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202499 !Sample_title = cy102_CD45pos_S260 !Sample_geo_accession = GSM3202499 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451069 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202500 !Sample_title = cy102_CD45neg_CD90neg_S335 !Sample_geo_accession = GSM3202500 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451068 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202501 !Sample_title = cy102_CD45pos_S214 !Sample_geo_accession = GSM3202501 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451067 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202502 !Sample_title = cy102_CD45neg_CD90neg_S336 !Sample_geo_accession = GSM3202502 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451066 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202503 !Sample_title = cy102_CD45pos_S228 !Sample_geo_accession = GSM3202503 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451065 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202504 !Sample_title = cy102_CD45pos_S202 !Sample_geo_accession = GSM3202504 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451064 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202505 !Sample_title = cy102_CD45neg_CD90neg_S323 !Sample_geo_accession = GSM3202505 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451063 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202506 !Sample_title = cy102_CD45pos_S197 !Sample_geo_accession = GSM3202506 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451062 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202507 !Sample_title = cy112_CD45neg_CD90neg_S171_9 !Sample_geo_accession = GSM3202507 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451061 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202508 !Sample_title = cy102_CD45pos_S247 !Sample_geo_accession = GSM3202508 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451060 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202509 !Sample_title = cy102_CD45pos_S243 !Sample_geo_accession = GSM3202509 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451617 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202510 !Sample_title = cy112_CD45pos_S75 !Sample_geo_accession = GSM3202510 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451616 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202511 !Sample_title = cy102_CD45neg_CD90neg_S374 !Sample_geo_accession = GSM3202511 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451688 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202512 !Sample_title = cy112_CD45pos_S90 !Sample_geo_accession = GSM3202512 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451687 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202513 !Sample_title = cy112_CD45pos_S48 !Sample_geo_accession = GSM3202513 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451686 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202514 !Sample_title = cy112_CD45pos_S57 !Sample_geo_accession = GSM3202514 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451685 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202515 !Sample_title = cy102_CD45pos_S245 !Sample_geo_accession = GSM3202515 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451684 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202516 !Sample_title = cy102_CD45neg_CD90neg_S337 !Sample_geo_accession = GSM3202516 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451683 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202517 !Sample_title = cy102_CD45neg_CD90neg_S327 !Sample_geo_accession = GSM3202517 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451682 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202518 !Sample_title = cy112_CD45pos_S67 !Sample_geo_accession = GSM3202518 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451681 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202519 !Sample_title = cy102_CD45pos_S287 !Sample_geo_accession = GSM3202519 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451680 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202520 !Sample_title = cy102_CD45neg_CD90neg_S308 !Sample_geo_accession = GSM3202520 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451334 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202521 !Sample_title = cy102_CD45neg_CD90neg_S356 !Sample_geo_accession = GSM3202521 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451333 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202522 !Sample_title = cy112_CD45pos_S28 !Sample_geo_accession = GSM3202522 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451332 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202523 !Sample_title = cy102_CD45neg_CD90neg_S341 !Sample_geo_accession = GSM3202523 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451331 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202524 !Sample_title = cy102_CD45neg_CD90neg_S361 !Sample_geo_accession = GSM3202524 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451330 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202525 !Sample_title = cy102_CD45pos_S265 !Sample_geo_accession = GSM3202525 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451329 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202526 !Sample_title = cy102_CD45pos_S229 !Sample_geo_accession = GSM3202526 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451328 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202527 !Sample_title = cy102_CD45neg_CD90neg_S383 !Sample_geo_accession = GSM3202527 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451327 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202528 !Sample_title = cy102_CD45pos_S226 !Sample_geo_accession = GSM3202528 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451326 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202529 !Sample_title = cy102_CD45neg_CD90neg_S368 !Sample_geo_accession = GSM3202529 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451325 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202530 !Sample_title = cy102_CD45neg_CD90neg_S375 !Sample_geo_accession = GSM3202530 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451399 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202531 !Sample_title = cy102_CD45neg_CD90neg_S366 !Sample_geo_accession = GSM3202531 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451398 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202532 !Sample_title = cy102_CD45neg_CD90neg_S369 !Sample_geo_accession = GSM3202532 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451397 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202533 !Sample_title = cy112_CD45pos_S25 !Sample_geo_accession = GSM3202533 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451396 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202534 !Sample_title = cy112_CD45pos_S19 !Sample_geo_accession = GSM3202534 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451395 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202535 !Sample_title = cy102_CD45pos_S246 !Sample_geo_accession = GSM3202535 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451394 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202536 !Sample_title = cy102_CD45neg_CD90neg_S350 !Sample_geo_accession = GSM3202536 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451393 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202537 !Sample_title = cy102_CD45pos_S193 !Sample_geo_accession = GSM3202537 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451392 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202538 !Sample_title = cy112_CD45pos_S70 !Sample_geo_accession = GSM3202538 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451391 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202539 !Sample_title = cy102_CD45neg_CD90neg_S322 !Sample_geo_accession = GSM3202539 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451390 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202540 !Sample_title = cy112_CD45neg_CD90neg_S188 !Sample_geo_accession = GSM3202540 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451389 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202541 !Sample_title = cy102_CD45pos_S263 !Sample_geo_accession = GSM3202541 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451388 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202542 !Sample_title = cy102_CD45neg_CD90neg_S339 !Sample_geo_accession = GSM3202542 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451387 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202543 !Sample_title = cy102_CD45pos_S285 !Sample_geo_accession = GSM3202543 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451386 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202544 !Sample_title = cy102_CD45neg_CD90neg_S349 !Sample_geo_accession = GSM3202544 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451385 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202545 !Sample_title = cy102_CD45pos_S256 !Sample_geo_accession = GSM3202545 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451384 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202546 !Sample_title = cy112_CD45pos_S95 !Sample_geo_accession = GSM3202546 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451383 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202547 !Sample_title = cy102_CD45pos_S230 !Sample_geo_accession = GSM3202547 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451382 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202548 !Sample_title = cy112_CD45pos_S53 !Sample_geo_accession = GSM3202548 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451381 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202549 !Sample_title = cy102_CD45pos_S270 !Sample_geo_accession = GSM3202549 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451380 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202550 !Sample_title = cy102_CD45neg_CD90neg_S304 !Sample_geo_accession = GSM3202550 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450918 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202551 !Sample_title = cy102_CD45neg_CD90neg_S320 !Sample_geo_accession = GSM3202551 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450913 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202552 !Sample_title = cy102_CD45pos_S281 !Sample_geo_accession = GSM3202552 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450912 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202553 !Sample_title = cy102_CD45neg_CD90neg_S351 !Sample_geo_accession = GSM3202553 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450911 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202554 !Sample_title = cy102_CD45pos_S208 !Sample_geo_accession = GSM3202554 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450917 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202555 !Sample_title = cy102_CD45pos_S242 !Sample_geo_accession = GSM3202555 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450916 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202556 !Sample_title = cy102_CD45pos_S269 !Sample_geo_accession = GSM3202556 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450915 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202557 !Sample_title = cy102_CD45pos_S218 !Sample_geo_accession = GSM3202557 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450914 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202558 !Sample_title = cy102_CD45neg_CD90neg_S306 !Sample_geo_accession = GSM3202558 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450910 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202559 !Sample_title = cy102_CD45pos_S210 !Sample_geo_accession = GSM3202559 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450909 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202560 !Sample_title = cy112_CD45neg_CD90neg_S149 !Sample_geo_accession = GSM3202560 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450908 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202561 !Sample_title = cy112_CD45pos_S10 !Sample_geo_accession = GSM3202561 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450907 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202562 !Sample_title = cy102_CD45pos_S255 !Sample_geo_accession = GSM3202562 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450906 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202563 !Sample_title = cy102_CD45neg_CD90neg_S325 !Sample_geo_accession = GSM3202563 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450905 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202564 !Sample_title = cy112_CD45pos_S43 !Sample_geo_accession = GSM3202564 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450904 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202565 !Sample_title = cy102_CD45neg_CD90neg_S367 !Sample_geo_accession = GSM3202565 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450903 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202566 !Sample_title = cy102_CD45pos_S273 !Sample_geo_accession = GSM3202566 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450902 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202567 !Sample_title = cy102_CD45neg_CD90neg_S292 !Sample_geo_accession = GSM3202567 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450901 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202568 !Sample_title = cy112_CD45pos_S52 !Sample_geo_accession = GSM3202568 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450900 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202569 !Sample_title = cy112_CD45pos_S23 !Sample_geo_accession = GSM3202569 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450899 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202570 !Sample_title = cy112_CD45pos_S16 !Sample_geo_accession = GSM3202570 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450898 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202571 !Sample_title = cy102_CD45neg_CD90neg_S338 !Sample_geo_accession = GSM3202571 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450897 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202572 !Sample_title = cy102_CD45neg_CD90neg_S328 !Sample_geo_accession = GSM3202572 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450896 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202573 !Sample_title = cy112_CD45pos_S20 !Sample_geo_accession = GSM3202573 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450895 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202574 !Sample_title = cy102_CD45pos_S268 !Sample_geo_accession = GSM3202574 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450894 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202575 !Sample_title = cy112_CD45pos_S79 !Sample_geo_accession = GSM3202575 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450893 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202576 !Sample_title = cy112_CD45pos_S54 !Sample_geo_accession = GSM3202576 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450964 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202577 !Sample_title = cy102_CD45pos_S280 !Sample_geo_accession = GSM3202577 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450963 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202578 !Sample_title = cy112_CD45pos_S32 !Sample_geo_accession = GSM3202578 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450962 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202579 !Sample_title = cy102_CD45pos_S213 !Sample_geo_accession = GSM3202579 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450961 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202580 !Sample_title = cy102_CD45neg_CD90neg_S296 !Sample_geo_accession = GSM3202580 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450844 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202581 !Sample_title = cy102_CD45pos_S227 !Sample_geo_accession = GSM3202581 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450839 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202582 !Sample_title = cy112_CD45pos_S64 !Sample_geo_accession = GSM3202582 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450838 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202583 !Sample_title = cy102_CD45neg_CD90neg_S373 !Sample_geo_accession = GSM3202583 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450837 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202584 !Sample_title = cy102_CD45neg_CD90neg_S347 !Sample_geo_accession = GSM3202584 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450843 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202585 !Sample_title = cy102_CD45pos_S215 !Sample_geo_accession = GSM3202585 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450842 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202586 !Sample_title = cy102_CD45neg_CD90neg_S353 !Sample_geo_accession = GSM3202586 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450841 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202587 !Sample_title = cy102_CD45pos_S203 !Sample_geo_accession = GSM3202587 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450840 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202588 !Sample_title = cy102_CD45neg_CD90neg_S355 !Sample_geo_accession = GSM3202588 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450836 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202589 !Sample_title = cy102_CD45neg_CD90neg_S352 !Sample_geo_accession = GSM3202589 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450835 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202590 !Sample_title = cy102_CD45neg_CD90neg_S309 !Sample_geo_accession = GSM3202590 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450834 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202591 !Sample_title = cy102_CD45neg_CD90neg_S305 !Sample_geo_accession = GSM3202591 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450833 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202592 !Sample_title = cy112_CD45neg_CD90neg_S129 !Sample_geo_accession = GSM3202592 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450832 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202593 !Sample_title = cy102_CD45pos_S258 !Sample_geo_accession = GSM3202593 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450831 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202594 !Sample_title = cy112_CD45pos_S92 !Sample_geo_accession = GSM3202594 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450830 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202595 !Sample_title = cy102_CD45pos_S266 !Sample_geo_accession = GSM3202595 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450829 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202596 !Sample_title = cy102_CD45neg_CD90neg_S346 !Sample_geo_accession = GSM3202596 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450828 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202597 !Sample_title = cy112_CD45pos_S1 !Sample_geo_accession = GSM3202597 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450892 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202598 !Sample_title = cy102_CD45neg_CD90neg_S294 !Sample_geo_accession = GSM3202598 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450891 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202599 !Sample_title = cy102_CD45neg_CD90neg_S329 !Sample_geo_accession = GSM3202599 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450890 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202600 !Sample_title = cy102_CD45pos_S264 !Sample_geo_accession = GSM3202600 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450889 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202601 !Sample_title = cy102_CD45neg_CD90neg_S303 !Sample_geo_accession = GSM3202601 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450888 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202602 !Sample_title = cy102_CD45neg_CD90neg_S333 !Sample_geo_accession = GSM3202602 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450887 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202603 !Sample_title = cy102_CD45pos_S277 !Sample_geo_accession = GSM3202603 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450886 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202604 !Sample_title = cy102_CD45pos_S257 !Sample_geo_accession = GSM3202604 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450885 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202605 !Sample_title = cy102_CD45pos_S231 !Sample_geo_accession = GSM3202605 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450884 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202606 !Sample_title = cy112_CD45neg_CD90neg_S132 !Sample_geo_accession = GSM3202606 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450883 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202607 !Sample_title = cy102_CD45neg_CD90neg_S297 !Sample_geo_accession = GSM3202607 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450882 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202608 !Sample_title = cy102_CD45neg_CD90neg_S326 !Sample_geo_accession = GSM3202608 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450881 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202609 !Sample_title = cy102_CD45pos_S251 !Sample_geo_accession = GSM3202609 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450880 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202610 !Sample_title = cy102_CD45neg_CD90neg_S321 !Sample_geo_accession = GSM3202610 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel102 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451155 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202611 !Sample_title = cy106_CD45pos_S277 !Sample_geo_accession = GSM3202611 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451154 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202612 !Sample_title = cy106_CD45pos_S199 !Sample_geo_accession = GSM3202612 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451153 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202613 !Sample_title = cy105_CD45pos_calceinpos_S21 !Sample_geo_accession = GSM3202613 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451152 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202614 !Sample_title = cy106_CD45neg_CD90neg_S363_10 !Sample_geo_accession = GSM3202614 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451151 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202615 !Sample_title = cy105_CD45pos_calceinpos_S20 !Sample_geo_accession = GSM3202615 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451150 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202616 !Sample_title = cy106_CD45pos_S220 !Sample_geo_accession = GSM3202616 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451149 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202617 !Sample_title = cy106_CD45pos_S239 !Sample_geo_accession = GSM3202617 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451148 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202618 !Sample_title = cy105_CD45pos_calceinpos_S13 !Sample_geo_accession = GSM3202618 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451147 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202619 !Sample_title = cy105_CD45pos_calceinpos_S94 !Sample_geo_accession = GSM3202619 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451146 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202620 !Sample_title = cy106_CD45neg_CD90neg_S309_11 !Sample_geo_accession = GSM3202620 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451218 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202621 !Sample_title = cy106_CD45neg_CD90neg_S378_12 !Sample_geo_accession = GSM3202621 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451217 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202622 !Sample_title = cy106_CD45pos_S263 !Sample_geo_accession = GSM3202622 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451216 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202623 !Sample_title = cy106_CD45pos_S227 !Sample_geo_accession = GSM3202623 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451215 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202624 !Sample_title = cy106_CD45neg_CD90neg_S333 !Sample_geo_accession = GSM3202624 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451214 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202625 !Sample_title = cy105_CD45pos_calceinpos_S88 !Sample_geo_accession = GSM3202625 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451213 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202626 !Sample_title = cy105_CD45pos_calceinpos_S80 !Sample_geo_accession = GSM3202626 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451212 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202627 !Sample_title = cy106_CD45pos_S194 !Sample_geo_accession = GSM3202627 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451211 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202628 !Sample_title = cy106_CD45neg_CD90neg_S304 !Sample_geo_accession = GSM3202628 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451210 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202629 !Sample_title = cy105_CD45pos_calceinpos_S54 !Sample_geo_accession = GSM3202629 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451209 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202630 !Sample_title = cy105_CD45pos_calceinpos_S15 !Sample_geo_accession = GSM3202630 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451208 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202631 !Sample_title = cy105_CD45neg_calceinpos_S152 !Sample_geo_accession = GSM3202631 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451207 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202632 !Sample_title = cy105_CD45pos_calceinpos_S16 !Sample_geo_accession = GSM3202632 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451206 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202633 !Sample_title = cy106_CD45pos_S213 !Sample_geo_accession = GSM3202633 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451205 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202634 !Sample_title = cy106_CD45pos_S280 !Sample_geo_accession = GSM3202634 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451204 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202635 !Sample_title = cy105_CD45pos_calceinpos_S90 !Sample_geo_accession = GSM3202635 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451203 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202636 !Sample_title = cy105_CD45pos_calceinpos_S41 !Sample_geo_accession = GSM3202636 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451202 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202637 !Sample_title = cy105_CD45pos_calceinpos_S71 !Sample_geo_accession = GSM3202637 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451201 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202638 !Sample_title = cy105_CD45neg_calceinpos_S179 !Sample_geo_accession = GSM3202638 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451200 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202639 !Sample_title = cy105_CD45pos_calceinpos_S9 !Sample_geo_accession = GSM3202639 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451199 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202640 !Sample_title = cy105_CD45pos_calceinpos_S61 !Sample_geo_accession = GSM3202640 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451198 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202641 !Sample_title = cy106_CD45neg_CD90neg_S369 !Sample_geo_accession = GSM3202641 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451197 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202642 !Sample_title = cy106_CD45pos_S211 !Sample_geo_accession = GSM3202642 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451196 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202643 !Sample_title = cy106_CD45pos_S269 !Sample_geo_accession = GSM3202643 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451195 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202644 !Sample_title = cy106_CD45neg_CD90neg_S322 !Sample_geo_accession = GSM3202644 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451194 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202645 !Sample_title = cy105_CD45pos_calceinpos_S4 !Sample_geo_accession = GSM3202645 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451193 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202646 !Sample_title = cy106_CD45neg_CD90neg_S330 !Sample_geo_accession = GSM3202646 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451192 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202647 !Sample_title = cy106_CD45neg_CD90neg_S316 !Sample_geo_accession = GSM3202647 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451191 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202648 !Sample_title = cy105_CD45pos_calceinpos_S75 !Sample_geo_accession = GSM3202648 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451190 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202649 !Sample_title = cy105_CD45pos_calceinpos_S49 !Sample_geo_accession = GSM3202649 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451189 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202650 !Sample_title = cy105_CD45pos_calceinpos_S3 !Sample_geo_accession = GSM3202650 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451188 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202651 !Sample_title = cy106_CD45pos_S261 !Sample_geo_accession = GSM3202651 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451187 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202652 !Sample_title = cy105_CD45pos_calceinpos_S76 !Sample_geo_accession = GSM3202652 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451186 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202653 !Sample_title = cy106_CD45pos_S237 !Sample_geo_accession = GSM3202653 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451185 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202654 !Sample_title = cy105_CD45pos_calceinpos_S95 !Sample_geo_accession = GSM3202654 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451184 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202655 !Sample_title = cy105_CD45pos_calceinpos_S45 !Sample_geo_accession = GSM3202655 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451183 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202656 !Sample_title = cy105_CD45pos_calceinpos_S6 !Sample_geo_accession = GSM3202656 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451182 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202657 !Sample_title = cy106_CD45pos_S256 !Sample_geo_accession = GSM3202657 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451255 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202658 !Sample_title = cy105_CD45pos_calceinpos_S33 !Sample_geo_accession = GSM3202658 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451254 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202659 !Sample_title = cy105_CD45pos_calceinpos_S89 !Sample_geo_accession = GSM3202659 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451253 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202660 !Sample_title = cy105_CD45pos_calceinpos_S93 !Sample_geo_accession = GSM3202660 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451252 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202661 !Sample_title = cy106_CD45neg_CD90neg_S292 !Sample_geo_accession = GSM3202661 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451251 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202662 !Sample_title = cy105_CD45pos_calceinpos_S77 !Sample_geo_accession = GSM3202662 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451250 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202663 !Sample_title = cy106_CD45pos_S216 !Sample_geo_accession = GSM3202663 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451249 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202664 !Sample_title = cy105_CD45pos_calceinpos_S73 !Sample_geo_accession = GSM3202664 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451248 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202665 !Sample_title = cy105_CD45neg_calceinpos_S192 !Sample_geo_accession = GSM3202665 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451247 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202666 !Sample_title = cy105_CD45pos_calceinpos_S82 !Sample_geo_accession = GSM3202666 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451246 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202667 !Sample_title = cy105_CD45pos_calceinpos_S37 !Sample_geo_accession = GSM3202667 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451245 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202668 !Sample_title = cy106_CD45neg_CD90neg_S355 !Sample_geo_accession = GSM3202668 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451244 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202669 !Sample_title = cy106_CD45pos_S234 !Sample_geo_accession = GSM3202669 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451243 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202670 !Sample_title = cy105_CD45pos_calceinpos_S31 !Sample_geo_accession = GSM3202670 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451524 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202671 !Sample_title = cy105_CD45pos_calceinpos_S14 !Sample_geo_accession = GSM3202671 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451523 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202672 !Sample_title = cy105_CD45neg_calceinpos_S190 !Sample_geo_accession = GSM3202672 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451522 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202673 !Sample_title = cy105_CD45pos_calceinpos_S92 !Sample_geo_accession = GSM3202673 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451521 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202674 !Sample_title = cy105_CD45pos_calceinpos_S23 !Sample_geo_accession = GSM3202674 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451520 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202675 !Sample_title = cy105_CD45pos_calceinpos_S53 !Sample_geo_accession = GSM3202675 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451519 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202676 !Sample_title = cy106_CD45pos_S197 !Sample_geo_accession = GSM3202676 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451518 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202677 !Sample_title = cy105_CD45neg_calceinpos_S132 !Sample_geo_accession = GSM3202677 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451517 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202678 !Sample_title = cy106_CD45neg_CD90neg_S336 !Sample_geo_accession = GSM3202678 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451516 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202679 !Sample_title = cy106_CD45pos_S285 !Sample_geo_accession = GSM3202679 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451515 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202680 !Sample_title = cy106_CD45pos_S231 !Sample_geo_accession = GSM3202680 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451514 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202681 !Sample_title = cy105_CD45pos_calceinpos_S69 !Sample_geo_accession = GSM3202681 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451513 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202682 !Sample_title = cy106_CD45neg_CD90neg_S317 !Sample_geo_accession = GSM3202682 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451512 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202683 !Sample_title = cy105_CD45pos_calceinpos_S27 !Sample_geo_accession = GSM3202683 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451511 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202684 !Sample_title = cy105_CD45pos_calceinpos_S84 !Sample_geo_accession = GSM3202684 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451510 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202685 !Sample_title = cy105_CD45pos_calceinpos_S60 !Sample_geo_accession = GSM3202685 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451580 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202686 !Sample_title = cy105_CD45pos_calceinpos_S8 !Sample_geo_accession = GSM3202686 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451579 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202687 !Sample_title = cy105_CD45pos_calceinpos_S42 !Sample_geo_accession = GSM3202687 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451578 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202688 !Sample_title = cy106_CD45neg_CD90neg_S321 !Sample_geo_accession = GSM3202688 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451577 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202689 !Sample_title = cy106_CD45neg_CD90neg_S289_13 !Sample_geo_accession = GSM3202689 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451576 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202690 !Sample_title = cy105_CD45pos_calceinpos_S28 !Sample_geo_accession = GSM3202690 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451575 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202691 !Sample_title = cy105_CD45neg_calceinpos_S189 !Sample_geo_accession = GSM3202691 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451574 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202692 !Sample_title = cy105_CD45pos_calceinpos_S38 !Sample_geo_accession = GSM3202692 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451573 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202693 !Sample_title = cy105_CD45pos_calceinpos_S26 !Sample_geo_accession = GSM3202693 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451572 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202694 !Sample_title = cy105_CD45pos_calceinpos_S2 !Sample_geo_accession = GSM3202694 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451571 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202695 !Sample_title = cy106_CD45pos_S275 !Sample_geo_accession = GSM3202695 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451570 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202696 !Sample_title = cy105_CD45neg_calceinpos_S118 !Sample_geo_accession = GSM3202696 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451569 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202697 !Sample_title = cy105_CD45pos_calceinpos_S19 !Sample_geo_accession = GSM3202697 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451568 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202698 !Sample_title = cy106_CD45neg_CD90neg_S300 !Sample_geo_accession = GSM3202698 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451567 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202699 !Sample_title = cy105_CD45pos_calceinpos_S25 !Sample_geo_accession = GSM3202699 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451566 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202700 !Sample_title = cy105_CD45pos_calceinpos_S91 !Sample_geo_accession = GSM3202700 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451606 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202701 !Sample_title = cy106_CD45pos_S260 !Sample_geo_accession = GSM3202701 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451605 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202702 !Sample_title = cy105_CD45pos_calceinpos_S59 !Sample_geo_accession = GSM3202702 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451600 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202703 !Sample_title = cy106_CD45pos_S283 !Sample_geo_accession = GSM3202703 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451599 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202704 !Sample_title = cy105_CD45pos_calceinpos_S78 !Sample_geo_accession = GSM3202704 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451604 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202705 !Sample_title = cy105_CD45pos_calceinpos_S7 !Sample_geo_accession = GSM3202705 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451603 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202706 !Sample_title = cy106_CD45pos_S254 !Sample_geo_accession = GSM3202706 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451602 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202707 !Sample_title = cy105_CD45pos_calceinpos_S66 !Sample_geo_accession = GSM3202707 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451601 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202708 !Sample_title = cy106_CD45pos_S273 !Sample_geo_accession = GSM3202708 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451598 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202709 !Sample_title = cy106_CD45pos_S222 !Sample_geo_accession = GSM3202709 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451597 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202710 !Sample_title = cy105_CD45pos_calceinpos_S79 !Sample_geo_accession = GSM3202710 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451596 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202711 !Sample_title = cy106_CD45pos_S210 !Sample_geo_accession = GSM3202711 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451595 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202712 !Sample_title = cy106_CD45neg_CD90neg_S313 !Sample_geo_accession = GSM3202712 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451594 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202713 !Sample_title = cy105_CD45pos_calceinpos_S81 !Sample_geo_accession = GSM3202713 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451593 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202714 !Sample_title = cy105_CD45pos_calceinpos_S64 !Sample_geo_accession = GSM3202714 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451592 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202715 !Sample_title = cy106_CD45neg_CD90neg_S365_14 !Sample_geo_accession = GSM3202715 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451591 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202716 !Sample_title = cy105_CD45neg_calceinpos_S130 !Sample_geo_accession = GSM3202716 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451590 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202717 !Sample_title = cy106_CD45pos_S235 !Sample_geo_accession = GSM3202717 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451589 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202718 !Sample_title = cy106_CD45neg_CD90neg_S357_15 !Sample_geo_accession = GSM3202718 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451588 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202719 !Sample_title = cy105_CD45pos_calceinpos_S11 !Sample_geo_accession = GSM3202719 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451587 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202720 !Sample_title = cy105_CD45neg_calceinpos_S182 !Sample_geo_accession = GSM3202720 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451586 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202721 !Sample_title = cy105_CD45pos_calceinpos_S56 !Sample_geo_accession = GSM3202721 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451585 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202722 !Sample_title = cy106_CD45pos_S208 !Sample_geo_accession = GSM3202722 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451584 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202723 !Sample_title = cy106_CD45neg_CD90neg_S354 !Sample_geo_accession = GSM3202723 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451583 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202724 !Sample_title = cy105_CD45pos_calceinpos_S40 !Sample_geo_accession = GSM3202724 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451582 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202725 !Sample_title = cy106_CD45pos_S282 !Sample_geo_accession = GSM3202725 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451581 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202726 !Sample_title = cy105_CD45pos_calceinpos_S5 !Sample_geo_accession = GSM3202726 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451654 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202727 !Sample_title = cy105_CD45pos_calceinpos_S96 !Sample_geo_accession = GSM3202727 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451653 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202728 !Sample_title = cy106_CD45pos_S230 !Sample_geo_accession = GSM3202728 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451651 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202729 !Sample_title = cy105_CD45neg_calceinpos_S191 !Sample_geo_accession = GSM3202729 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451283 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202730 !Sample_title = cy105_CD45pos_calceinpos_S50 !Sample_geo_accession = GSM3202730 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451282 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202731 !Sample_title = cy106_CD45pos_S248 !Sample_geo_accession = GSM3202731 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451281 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202732 !Sample_title = cy105_CD45pos_calceinpos_S52 !Sample_geo_accession = GSM3202732 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451280 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202733 !Sample_title = cy105_CD45neg_calceinpos_S131 !Sample_geo_accession = GSM3202733 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451279 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202734 !Sample_title = cy105_CD45pos_calceinpos_S39 !Sample_geo_accession = GSM3202734 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451652 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202735 !Sample_title = cy105_CD45pos_calceinpos_S17 !Sample_geo_accession = GSM3202735 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451278 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202736 !Sample_title = cy106_CD45pos_S264 !Sample_geo_accession = GSM3202736 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451277 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202737 !Sample_title = cy105_CD45pos_calceinpos_S87 !Sample_geo_accession = GSM3202737 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451276 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202738 !Sample_title = cy105_CD45pos_calceinpos_S29 !Sample_geo_accession = GSM3202738 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451275 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202739 !Sample_title = cy105_CD45pos_calceinpos_S51 !Sample_geo_accession = GSM3202739 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451274 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202740 !Sample_title = cy105_CD45pos_calceinpos_S1 !Sample_geo_accession = GSM3202740 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451273 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202741 !Sample_title = cy105_CD45neg_calceinpos_S108 !Sample_geo_accession = GSM3202741 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451272 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202742 !Sample_title = cy106_CD45pos_S252 !Sample_geo_accession = GSM3202742 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451271 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202743 !Sample_title = cy106_CD45neg_CD90neg_S380 !Sample_geo_accession = GSM3202743 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451270 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202744 !Sample_title = cy105_CD45pos_calceinpos_S86 !Sample_geo_accession = GSM3202744 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451269 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202745 !Sample_title = cy106_CD45pos_S242 !Sample_geo_accession = GSM3202745 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451268 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202746 !Sample_title = cy105_CD45pos_calceinpos_S18 !Sample_geo_accession = GSM3202746 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451267 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202747 !Sample_title = cy106_CD45neg_CD90neg_S350 !Sample_geo_accession = GSM3202747 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451266 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202748 !Sample_title = cy106_CD45neg_CD90neg_S344_16 !Sample_geo_accession = GSM3202748 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451265 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202749 !Sample_title = cy106_CD45pos_S215 !Sample_geo_accession = GSM3202749 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451264 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202750 !Sample_title = cy106_CD45pos_S193 !Sample_geo_accession = GSM3202750 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451263 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202751 !Sample_title = cy105_CD45pos_calceinpos_S70 !Sample_geo_accession = GSM3202751 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451262 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202752 !Sample_title = cy106_CD45pos_S262 !Sample_geo_accession = GSM3202752 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451261 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202753 !Sample_title = cy106_CD45neg_CD90neg_S306_17 !Sample_geo_accession = GSM3202753 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451260 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202754 !Sample_title = cy106_CD45pos_S249 !Sample_geo_accession = GSM3202754 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451259 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202755 !Sample_title = cy106_CD45pos_S251 !Sample_geo_accession = GSM3202755 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451258 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202756 !Sample_title = cy106_CD45pos_S250 !Sample_geo_accession = GSM3202756 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451257 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202757 !Sample_title = cy106_CD45pos_S201 !Sample_geo_accession = GSM3202757 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451256 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202758 !Sample_title = cy105_CD45pos_calceinpos_S30 !Sample_geo_accession = GSM3202758 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451324 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202759 !Sample_title = cy106_CD45pos_S244 !Sample_geo_accession = GSM3202759 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451323 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202760 !Sample_title = cy105_CD45pos_calceinpos_S85 !Sample_geo_accession = GSM3202760 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451242 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202761 !Sample_title = cy106_CD45neg_CD90neg_S295 !Sample_geo_accession = GSM3202761 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451241 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202762 !Sample_title = cy105_CD45pos_calceinpos_S55 !Sample_geo_accession = GSM3202762 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451240 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202763 !Sample_title = cy105_CD45pos_calceinpos_S83 !Sample_geo_accession = GSM3202763 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451239 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202764 !Sample_title = cy105_CD45pos_calceinpos_S62 !Sample_geo_accession = GSM3202764 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel105 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451238 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202765 !Sample_title = cy106_CD45neg_CD90neg_S368 !Sample_geo_accession = GSM3202765 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451237 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202766 !Sample_title = cy110_CD45neg_CD90neg_S12 !Sample_geo_accession = GSM3202766 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451236 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202767 !Sample_title = cy110_CD45neg_CD90neg_S60 !Sample_geo_accession = GSM3202767 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451235 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202768 !Sample_title = cy110_CD45neg_CD90neg_S56 !Sample_geo_accession = GSM3202768 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451234 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202769 !Sample_title = cy110_CD45neg_CD90neg_S86 !Sample_geo_accession = GSM3202769 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451233 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202770 !Sample_title = cy98_CD45pos_1_S202 !Sample_geo_accession = GSM3202770 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451232 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202771 !Sample_title = cy112_CD45neg_CD90neg_S128 !Sample_geo_accession = GSM3202771 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451231 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202772 !Sample_title = cy98_CD45pos_1_S200 !Sample_geo_accession = GSM3202772 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451230 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202773 !Sample_title = cy98_CD45pos_2_S310 !Sample_geo_accession = GSM3202773 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451229 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202774 !Sample_title = cy98_CD45pos_2_S318 !Sample_geo_accession = GSM3202774 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451228 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202775 !Sample_title = cy98_CD45pos_1_S231 !Sample_geo_accession = GSM3202775 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451227 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202776 !Sample_title = cy98_CD45pos_2_S319 !Sample_geo_accession = GSM3202776 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451226 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202777 !Sample_title = cy112_CD45neg_CD90neg_S112 !Sample_geo_accession = GSM3202777 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451225 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202778 !Sample_title = cy110_CD45neg_CD90neg_S11 !Sample_geo_accession = GSM3202778 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451224 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202779 !Sample_title = cy110_CD45neg_CD90neg_S29 !Sample_geo_accession = GSM3202779 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451223 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202780 !Sample_title = cy112_CD45neg_CD90neg_S137 !Sample_geo_accession = GSM3202780 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451222 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202781 !Sample_title = cy98_CD45pos_2_S347 !Sample_geo_accession = GSM3202781 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451221 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202782 !Sample_title = cy110_CD45neg_CD90neg_S45 !Sample_geo_accession = GSM3202782 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451220 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202783 !Sample_title = cy98_CD45pos_2_S326 !Sample_geo_accession = GSM3202783 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451219 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202784 !Sample_title = cy98_CD45pos_2_S376 !Sample_geo_accession = GSM3202784 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451289 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202785 !Sample_title = cy98_CD45pos_2_S304 !Sample_geo_accession = GSM3202785 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451288 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202786 !Sample_title = cy98_CD45pos_2_S315 !Sample_geo_accession = GSM3202786 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451287 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202787 !Sample_title = cy98_CD45pos_2_S349 !Sample_geo_accession = GSM3202787 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451286 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202788 !Sample_title = cy98_CD45pos_2_S362 !Sample_geo_accession = GSM3202788 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451285 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202789 !Sample_title = cy98_CD45pos_1_S233 !Sample_geo_accession = GSM3202789 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451284 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202790 !Sample_title = cy110_CD45neg_CD90neg_S72 !Sample_geo_accession = GSM3202790 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451422 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202791 !Sample_title = cy98_CD45pos_1_S197 !Sample_geo_accession = GSM3202791 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451421 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202792 !Sample_title = cy98_CD45pos_1_S223 !Sample_geo_accession = GSM3202792 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451420 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202793 !Sample_title = cy98_CD45pos_1_S201 !Sample_geo_accession = GSM3202793 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451419 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202794 !Sample_title = cy110_CD45neg_CD90neg_S8 !Sample_geo_accession = GSM3202794 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451418 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202795 !Sample_title = cy98_CD45pos_2_S369 !Sample_geo_accession = GSM3202795 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451417 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202796 !Sample_title = cy110_CD45neg_CD90neg_S35 !Sample_geo_accession = GSM3202796 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451416 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202797 !Sample_title = cy98_CD45pos_1_S240 !Sample_geo_accession = GSM3202797 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451415 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202798 !Sample_title = cy110_CD45neg_CD90neg_S42 !Sample_geo_accession = GSM3202798 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451414 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202799 !Sample_title = cy98_CD45pos_2_S382 !Sample_geo_accession = GSM3202799 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451413 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202800 !Sample_title = cy98_CD45pos_1_S237 !Sample_geo_accession = GSM3202800 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451412 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202801 !Sample_title = cy98_CD45pos_2_S295 !Sample_geo_accession = GSM3202801 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451411 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202802 !Sample_title = cy98_CD45pos_1_S236 !Sample_geo_accession = GSM3202802 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451410 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202803 !Sample_title = cy98_CD45pos_1_S208 !Sample_geo_accession = GSM3202803 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451409 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202804 !Sample_title = cy112_CD45neg_CD90neg_S121 !Sample_geo_accession = GSM3202804 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451408 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202805 !Sample_title = cy112_CD45neg_CD90neg_S143 !Sample_geo_accession = GSM3202805 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451407 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202806 !Sample_title = cy110_CD45neg_CD90neg_S52 !Sample_geo_accession = GSM3202806 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451406 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202807 !Sample_title = cy98_CD45pos_2_S345 !Sample_geo_accession = GSM3202807 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451405 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202808 !Sample_title = cy110_CD45neg_CD90neg_S61 !Sample_geo_accession = GSM3202808 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451404 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202809 !Sample_title = cy98_CD45pos_2_S375 !Sample_geo_accession = GSM3202809 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451403 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202810 !Sample_title = cy98_CD45pos_2_S339 !Sample_geo_accession = GSM3202810 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451402 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202811 !Sample_title = cy112_CD45neg_CD90neg_S110 !Sample_geo_accession = GSM3202811 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451401 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202812 !Sample_title = cy98_CD45pos_2_S312 !Sample_geo_accession = GSM3202812 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451400 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202813 !Sample_title = cy98_CD45pos_2_S327 !Sample_geo_accession = GSM3202813 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451477 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202814 !Sample_title = cy98_CD45pos_2_S308 !Sample_geo_accession = GSM3202814 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451476 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202815 !Sample_title = cy98_CD45pos_2_S305 !Sample_geo_accession = GSM3202815 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451475 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202816 !Sample_title = cy98_CD45pos_2_S378 !Sample_geo_accession = GSM3202816 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451474 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202817 !Sample_title = cy98_CD45pos_2_S329 !Sample_geo_accession = GSM3202817 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451379 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202818 !Sample_title = cy98_CD45pos_2_S332 !Sample_geo_accession = GSM3202818 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451378 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202819 !Sample_title = cy98_CD45pos_1_S198 !Sample_geo_accession = GSM3202819 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451377 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202820 !Sample_title = cy110_CD45neg_CD90neg_S7 !Sample_geo_accession = GSM3202820 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451376 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202821 !Sample_title = cy98_CD45pos_2_S293 !Sample_geo_accession = GSM3202821 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451372 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202822 !Sample_title = cy98_CD45pos_1_S212 !Sample_geo_accession = GSM3202822 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451371 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202823 !Sample_title = cy110_CD45neg_CD90neg_S34 !Sample_geo_accession = GSM3202823 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451370 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202824 !Sample_title = cy112_CD45neg_CD90neg_S126 !Sample_geo_accession = GSM3202824 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451375 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202825 !Sample_title = cy98_CD45pos_2_S309 !Sample_geo_accession = GSM3202825 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451374 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202826 !Sample_title = cy110_CD45neg_CD90neg_S46 !Sample_geo_accession = GSM3202826 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451373 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202827 !Sample_title = cy110_CD45neg_CD90neg_S78 !Sample_geo_accession = GSM3202827 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451369 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202828 !Sample_title = cy98_CD45pos_2_S323 !Sample_geo_accession = GSM3202828 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451368 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202829 !Sample_title = cy98_CD45pos_1_S216 !Sample_geo_accession = GSM3202829 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451367 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202830 !Sample_title = cy98_CD45pos_2_S313 !Sample_geo_accession = GSM3202830 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451366 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202831 !Sample_title = cy98_CD45pos_1_S209 !Sample_geo_accession = GSM3202831 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451365 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202832 !Sample_title = cy98_CD45pos_2_S351 !Sample_geo_accession = GSM3202832 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451364 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202833 !Sample_title = cy98_CD45pos_2_S301 !Sample_geo_accession = GSM3202833 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451363 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202834 !Sample_title = cy98_CD45pos_1_S219 !Sample_geo_accession = GSM3202834 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451362 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202835 !Sample_title = cy110_CD45neg_CD90neg_S62 !Sample_geo_accession = GSM3202835 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451434 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202836 !Sample_title = cy98_CD45pos_2_S334 !Sample_geo_accession = GSM3202836 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451433 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202837 !Sample_title = cy98_CD45pos_2_S303 !Sample_geo_accession = GSM3202837 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451432 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202838 !Sample_title = cy98_CD45pos_2_S352 !Sample_geo_accession = GSM3202838 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451431 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202839 !Sample_title = cy98_CD45pos_2_S292 !Sample_geo_accession = GSM3202839 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451430 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202840 !Sample_title = cy98_CD45pos_2_S366 !Sample_geo_accession = GSM3202840 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451429 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202841 !Sample_title = cy98_CD45pos_1_S204 !Sample_geo_accession = GSM3202841 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451428 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202842 !Sample_title = cy112_CD45neg_CD90neg_S170 !Sample_geo_accession = GSM3202842 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451427 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202843 !Sample_title = cy98_CD45pos_2_S290 !Sample_geo_accession = GSM3202843 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451426 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202844 !Sample_title = cy98_CD45pos_1_S218 !Sample_geo_accession = GSM3202844 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451425 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202845 !Sample_title = cy98_CD45pos_2_S360 !Sample_geo_accession = GSM3202845 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451424 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202846 !Sample_title = cy98_CD45pos_1_S210 !Sample_geo_accession = GSM3202846 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451423 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202847 !Sample_title = cy98_CD45pos_2_S344 !Sample_geo_accession = GSM3202847 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451292 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202848 !Sample_title = cy98_CD45pos_2_S335 !Sample_geo_accession = GSM3202848 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451291 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202849 !Sample_title = cy110_CD45neg_CD90neg_S74 !Sample_geo_accession = GSM3202849 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451290 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202850 !Sample_title = cy110_CD45neg_CD90neg_S26 !Sample_geo_accession = GSM3202850 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451361 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202851 !Sample_title = cy112_CD45neg_CD90neg_S171_18 !Sample_geo_accession = GSM3202851 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451360 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202852 !Sample_title = cy98_CD45pos_2_S299 !Sample_geo_accession = GSM3202852 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451359 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202853 !Sample_title = cy110_CD45neg_CD90neg_S17 !Sample_geo_accession = GSM3202853 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451358 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202854 !Sample_title = cy98_CD45pos_2_S297 !Sample_geo_accession = GSM3202854 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451357 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202855 !Sample_title = cy98_CD45pos_1_S215 !Sample_geo_accession = GSM3202855 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451356 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202856 !Sample_title = cy98_CD45pos_2_S314 !Sample_geo_accession = GSM3202856 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451355 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202857 !Sample_title = cy98_CD45pos_2_S380 !Sample_geo_accession = GSM3202857 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451354 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202858 !Sample_title = cy98_CD45pos_1_S195 !Sample_geo_accession = GSM3202858 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451353 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202859 !Sample_title = cy98_CD45pos_2_S356 !Sample_geo_accession = GSM3202859 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451352 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202860 !Sample_title = cy98_CD45pos_1_S213 !Sample_geo_accession = GSM3202860 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451351 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202861 !Sample_title = cy98_CD45pos_2_S307 !Sample_geo_accession = GSM3202861 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451350 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202862 !Sample_title = cy98_CD45pos_2_S300 !Sample_geo_accession = GSM3202862 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451349 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202863 !Sample_title = cy98_CD45pos_2_S336 !Sample_geo_accession = GSM3202863 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451348 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202864 !Sample_title = cy98_CD45pos_2_S331 !Sample_geo_accession = GSM3202864 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451347 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202865 !Sample_title = cy98_CD45pos_1_S194 !Sample_geo_accession = GSM3202865 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451346 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202866 !Sample_title = cy110_CD45neg_CD90neg_S92 !Sample_geo_accession = GSM3202866 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451345 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202867 !Sample_title = cy98_CD45pos_2_S365 !Sample_geo_accession = GSM3202867 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451344 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202868 !Sample_title = cy98_CD45pos_2_S296 !Sample_geo_accession = GSM3202868 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451343 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202869 !Sample_title = cy110_CD45neg_CD90neg_S80 !Sample_geo_accession = GSM3202869 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451342 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202870 !Sample_title = cy110_CD45neg_CD90neg_S69 !Sample_geo_accession = GSM3202870 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451341 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202871 !Sample_title = cy110_CD45neg_CD90neg_S79 !Sample_geo_accession = GSM3202871 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451340 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202872 !Sample_title = cy98_CD45pos_2_S338 !Sample_geo_accession = GSM3202872 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451339 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202873 !Sample_title = cy98_CD45pos_1_S235 !Sample_geo_accession = GSM3202873 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451338 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202874 !Sample_title = cy110_CD45neg_CD90neg_S64 !Sample_geo_accession = GSM3202874 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451337 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202875 !Sample_title = cy98_CD45pos_1_S220 !Sample_geo_accession = GSM3202875 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451336 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202876 !Sample_title = cy98_CD45pos_2_S321 !Sample_geo_accession = GSM3202876 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451335 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202877 !Sample_title = cy98_CD45pos_1_S222 !Sample_geo_accession = GSM3202877 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452704 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202878 !Sample_title = cy98_CD45pos_2_S317 !Sample_geo_accession = GSM3202878 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452703 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202879 !Sample_title = cy112_CD45neg_CD90neg_S103 !Sample_geo_accession = GSM3202879 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452702 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202880 !Sample_title = cy98_CD45pos_2_S346 !Sample_geo_accession = GSM3202880 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452701 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202881 !Sample_title = cy98_CD45pos_2_S367 !Sample_geo_accession = GSM3202881 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452700 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202882 !Sample_title = cy112_CD45neg_CD90neg_S97 !Sample_geo_accession = GSM3202882 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel112 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452699 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202883 !Sample_title = cy110_CD45neg_CD90neg_S43 !Sample_geo_accession = GSM3202883 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452698 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202884 !Sample_title = cy98_CD45pos_2_S350 !Sample_geo_accession = GSM3202884 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452697 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202885 !Sample_title = cy98_CD45pos_2_S373 !Sample_geo_accession = GSM3202885 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452696 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202886 !Sample_title = cy98_CD45pos_1_S211 !Sample_geo_accession = GSM3202886 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452695 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202887 !Sample_title = cy98_CD45pos_2_S363 !Sample_geo_accession = GSM3202887 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452694 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202888 !Sample_title = cy98_CD45pos_1_S214 !Sample_geo_accession = GSM3202888 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452693 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202889 !Sample_title = cy110_CD45neg_CD90neg_S50 !Sample_geo_accession = GSM3202889 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452692 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202890 !Sample_title = cy98_CD45pos_1_S239 !Sample_geo_accession = GSM3202890 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452691 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202891 !Sample_title = cy110_CD45neg_CD90neg_S3 !Sample_geo_accession = GSM3202891 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452690 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202892 !Sample_title = cy98_CD45pos_1_S226 !Sample_geo_accession = GSM3202892 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452689 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202893 !Sample_title = cy110_CD45neg_CD90neg_S54 !Sample_geo_accession = GSM3202893 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452688 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202894 !Sample_title = cy98_CD45pos_1_S196 !Sample_geo_accession = GSM3202894 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452687 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202895 !Sample_title = cy98_CD45pos_2_S289 !Sample_geo_accession = GSM3202895 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452686 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202896 !Sample_title = cy98_CD45pos_2_S348 !Sample_geo_accession = GSM3202896 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452685 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202897 !Sample_title = cy98_CD45pos_2_S322 !Sample_geo_accession = GSM3202897 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452684 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202898 !Sample_title = cy98_CD45pos_2_S357 !Sample_geo_accession = GSM3202898 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452683 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202899 !Sample_title = cy98_CD45pos_2_S354 !Sample_geo_accession = GSM3202899 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452753 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202900 !Sample_title = cy98_CD45pos_2_S379 !Sample_geo_accession = GSM3202900 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452752 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202901 !Sample_title = cy98_CD45pos_2_S374 !Sample_geo_accession = GSM3202901 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452751 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202902 !Sample_title = cy98_CD45pos_2_S330 !Sample_geo_accession = GSM3202902 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452750 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202903 !Sample_title = cy98_CD45pos_2_S342 !Sample_geo_accession = GSM3202903 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452749 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202904 !Sample_title = cy98_CD45pos_2_S311 !Sample_geo_accession = GSM3202904 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452748 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202905 !Sample_title = cy98_CD45pos_2_S355 !Sample_geo_accession = GSM3202905 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452747 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202906 !Sample_title = cy98_CD45pos_2_S383 !Sample_geo_accession = GSM3202906 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452746 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202907 !Sample_title = cy98_CD45pos_2_S368 !Sample_geo_accession = GSM3202907 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451650 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202908 !Sample_title = cy98_CD45pos_1_S230 !Sample_geo_accession = GSM3202908 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451649 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202909 !Sample_title = cy98_CD45pos_2_S358 !Sample_geo_accession = GSM3202909 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451648 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202910 !Sample_title = cy98_CD45pos_1_S193 !Sample_geo_accession = GSM3202910 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451647 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202911 !Sample_title = cy98_CD45pos_1_S234 !Sample_geo_accession = GSM3202911 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451646 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202912 !Sample_title = cy98_CD45pos_2_S372 !Sample_geo_accession = GSM3202912 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451645 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202913 !Sample_title = cy110_CD45neg_CD90neg_S96 !Sample_geo_accession = GSM3202913 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451644 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202914 !Sample_title = cy98_CD45pos_2_S340 !Sample_geo_accession = GSM3202914 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451643 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202915 !Sample_title = cy98_CD45pos_2_S364 !Sample_geo_accession = GSM3202915 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451642 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202916 !Sample_title = cy98_CD45pos_2_S359 !Sample_geo_accession = GSM3202916 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451641 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202917 !Sample_title = cy110_CD45neg_CD90neg_S95 !Sample_geo_accession = GSM3202917 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451640 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202918 !Sample_title = cy98_CD45pos_1_S207 !Sample_geo_accession = GSM3202918 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451639 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202919 !Sample_title = cy110_CD45neg_CD90neg_S21 !Sample_geo_accession = GSM3202919 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451638 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202920 !Sample_title = cy98_CD45pos_2_S333 !Sample_geo_accession = GSM3202920 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451637 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202921 !Sample_title = cy98_CD45pos_2_S291 !Sample_geo_accession = GSM3202921 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451636 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202922 !Sample_title = cy98_CD45pos_1_S225 !Sample_geo_accession = GSM3202922 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451635 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202923 !Sample_title = cy98_CD45pos_2_S298 !Sample_geo_accession = GSM3202923 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451634 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202924 !Sample_title = cy110_CD45neg_CD90neg_S68 !Sample_geo_accession = GSM3202924 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451633 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202925 !Sample_title = cy98_CD45pos_2_S302 !Sample_geo_accession = GSM3202925 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451629 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202926 !Sample_title = cy98_CD45pos_2_S337 !Sample_geo_accession = GSM3202926 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel98 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451628 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202927 !Sample_title = cy103_CD45pos_S225 !Sample_geo_accession = GSM3202927 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451627 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202928 !Sample_title = cy110_CD45neg_CD90neg_S135 !Sample_geo_accession = GSM3202928 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451626 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202929 !Sample_title = cy103_CD45neg_CD90neg_S60 !Sample_geo_accession = GSM3202929 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451625 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202930 !Sample_title = cy103_CD45neg_CD90neg_S28 !Sample_geo_accession = GSM3202930 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451624 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202931 !Sample_title = cy110_CD45pos_S331 !Sample_geo_accession = GSM3202931 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451623 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202932 !Sample_title = cy110_CD45neg_CD90neg_S184 !Sample_geo_accession = GSM3202932 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451622 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202933 !Sample_title = cy103_CD45neg_CD90neg_S68 !Sample_geo_accession = GSM3202933 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451621 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202934 !Sample_title = cy103_CD45neg_CD90neg_S16 !Sample_geo_accession = GSM3202934 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451620 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202935 !Sample_title = cy110_CD45pos_S297 !Sample_geo_accession = GSM3202935 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451619 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202936 !Sample_title = cy103_CD45pos_S282 !Sample_geo_accession = GSM3202936 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451618 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202937 !Sample_title = cy103_CD45neg_CD90neg_S18 !Sample_geo_accession = GSM3202937 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451679 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202938 !Sample_title = cy110_CD45neg_CD90neg_S190 !Sample_geo_accession = GSM3202938 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451678 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202939 !Sample_title = cy110_CD45pos_S342 !Sample_geo_accession = GSM3202939 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451677 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202940 !Sample_title = cy110_CD45pos_S329 !Sample_geo_accession = GSM3202940 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451676 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202941 !Sample_title = cy103_CD45pos_S224 !Sample_geo_accession = GSM3202941 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451675 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202942 !Sample_title = cy110_CD45neg_CD90neg_S120 !Sample_geo_accession = GSM3202942 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451674 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202943 !Sample_title = cy103_CD45neg_CD90neg_S64 !Sample_geo_accession = GSM3202943 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451673 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202944 !Sample_title = cy110_CD45pos_S308 !Sample_geo_accession = GSM3202944 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451672 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202945 !Sample_title = cy103_CD45pos_S195 !Sample_geo_accession = GSM3202945 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451671 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202946 !Sample_title = cy103_CD45neg_CD90neg_S1 !Sample_geo_accession = GSM3202946 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451670 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202947 !Sample_title = cy103_CD45neg_CD90neg_S30 !Sample_geo_accession = GSM3202947 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451669 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202948 !Sample_title = cy103_CD45pos_S247 !Sample_geo_accession = GSM3202948 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451668 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202949 !Sample_title = cy110_CD45pos_S358 !Sample_geo_accession = GSM3202949 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451667 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202950 !Sample_title = cy110_CD45pos_S313 !Sample_geo_accession = GSM3202950 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451666 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202951 !Sample_title = cy103_CD45pos_S278 !Sample_geo_accession = GSM3202951 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451665 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202952 !Sample_title = cy103_CD45pos_S204 !Sample_geo_accession = GSM3202952 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451664 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202953 !Sample_title = cy103_CD45neg_CD90neg_S25 !Sample_geo_accession = GSM3202953 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451663 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202954 !Sample_title = cy110_CD45pos_S332 !Sample_geo_accession = GSM3202954 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451565 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202955 !Sample_title = cy103_CD45pos_S222 !Sample_geo_accession = GSM3202955 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451564 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202956 !Sample_title = cy103_CD45pos_S220 !Sample_geo_accession = GSM3202956 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451563 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202957 !Sample_title = cy103_CD45pos_S265 !Sample_geo_accession = GSM3202957 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451562 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202958 !Sample_title = cy103_CD45neg_CD90neg_S77 !Sample_geo_accession = GSM3202958 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451561 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202959 !Sample_title = cy103_CD45neg_CD90neg_S13 !Sample_geo_accession = GSM3202959 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451560 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202960 !Sample_title = cy103_CD45pos_S196 !Sample_geo_accession = GSM3202960 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451559 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202961 !Sample_title = cy103_CD45pos_S268 !Sample_geo_accession = GSM3202961 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451558 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202962 !Sample_title = cy103_CD45neg_CD90neg_S27 !Sample_geo_accession = GSM3202962 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451557 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202963 !Sample_title = cy103_CD45neg_CD90neg_S35 !Sample_geo_accession = GSM3202963 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451556 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202964 !Sample_title = cy110_CD45pos_S336 !Sample_geo_accession = GSM3202964 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451555 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202965 !Sample_title = cy103_CD45pos_S267 !Sample_geo_accession = GSM3202965 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451554 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202966 !Sample_title = cy110_CD45neg_CD90neg_S115 !Sample_geo_accession = GSM3202966 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451553 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202967 !Sample_title = cy110_CD45pos_S352 !Sample_geo_accession = GSM3202967 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451552 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202968 !Sample_title = cy103_CD45neg_CD90neg_S45 !Sample_geo_accession = GSM3202968 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451551 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202969 !Sample_title = cy110_CD45pos_S381 !Sample_geo_accession = GSM3202969 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451550 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202970 !Sample_title = cy103_CD45neg_CD90neg_S52 !Sample_geo_accession = GSM3202970 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451549 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202971 !Sample_title = cy110_CD45pos_S303 !Sample_geo_accession = GSM3202971 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451548 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202972 !Sample_title = cy103_CD45pos_S253 !Sample_geo_accession = GSM3202972 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451547 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202973 !Sample_title = cy110_CD45pos_S339 !Sample_geo_accession = GSM3202973 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451546 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202974 !Sample_title = cy103_CD45pos_S273 !Sample_geo_accession = GSM3202974 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451545 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202975 !Sample_title = cy103_CD45pos_S245 !Sample_geo_accession = GSM3202975 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451615 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202976 !Sample_title = cy103_CD45pos_S260 !Sample_geo_accession = GSM3202976 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451614 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202977 !Sample_title = cy110_CD45neg_CD90neg_S117 !Sample_geo_accession = GSM3202977 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451613 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202978 !Sample_title = cy110_CD45pos_S363 !Sample_geo_accession = GSM3202978 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451612 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202979 !Sample_title = cy110_CD45pos_S292 !Sample_geo_accession = GSM3202979 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451611 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202980 !Sample_title = cy103_CD45pos_S199 !Sample_geo_accession = GSM3202980 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451610 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202981 !Sample_title = cy103_CD45pos_S230 !Sample_geo_accession = GSM3202981 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451609 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202982 !Sample_title = cy103_CD45pos_S249 !Sample_geo_accession = GSM3202982 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451608 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202983 !Sample_title = cy110_CD45pos_S326 !Sample_geo_accession = GSM3202983 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451607 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202984 !Sample_title = cy103_CD45pos_S250 !Sample_geo_accession = GSM3202984 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452606 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202985 !Sample_title = cy103_CD45pos_S248 !Sample_geo_accession = GSM3202985 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452605 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202986 !Sample_title = cy103_CD45neg_CD90neg_S24 !Sample_geo_accession = GSM3202986 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452604 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202987 !Sample_title = cy103_CD45neg_CD90neg_S11 !Sample_geo_accession = GSM3202987 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452603 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202988 !Sample_title = cy110_CD45pos_S378 !Sample_geo_accession = GSM3202988 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452682 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202989 !Sample_title = cy110_CD45pos_S370 !Sample_geo_accession = GSM3202989 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452681 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202990 !Sample_title = cy110_CD45neg_CD90neg_S132 !Sample_geo_accession = GSM3202990 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452680 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202991 !Sample_title = cy110_CD45neg_CD90neg_S128 !Sample_geo_accession = GSM3202991 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452679 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202992 !Sample_title = cy103_CD45neg_CD90neg_S87 !Sample_geo_accession = GSM3202992 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452678 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202993 !Sample_title = cy103_CD45pos_S229 !Sample_geo_accession = GSM3202993 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452677 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202994 !Sample_title = cy103_CD45pos_S285 !Sample_geo_accession = GSM3202994 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452676 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202995 !Sample_title = cy103_CD45pos_S264 !Sample_geo_accession = GSM3202995 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452675 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202996 !Sample_title = cy103_CD45neg_CD90neg_S94 !Sample_geo_accession = GSM3202996 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452674 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202997 !Sample_title = cy103_CD45neg_CD90neg_S57 !Sample_geo_accession = GSM3202997 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452673 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202998 !Sample_title = cy110_CD45neg_CD90neg_S97 !Sample_geo_accession = GSM3202998 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452672 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3202999 !Sample_title = cy110_CD45neg_CD90neg_S123 !Sample_geo_accession = GSM3202999 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452671 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203000 !Sample_title = cy103_CD45neg_CD90neg_S7 !Sample_geo_accession = GSM3203000 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452670 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203001 !Sample_title = cy103_CD45neg_CD90neg_S49 !Sample_geo_accession = GSM3203001 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452669 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203002 !Sample_title = cy103_CD45neg_CD90neg_S58 !Sample_geo_accession = GSM3203002 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452668 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203003 !Sample_title = cy103_CD45neg_CD90neg_S20 !Sample_geo_accession = GSM3203003 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452667 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203004 !Sample_title = cy110_CD45pos_S290 !Sample_geo_accession = GSM3203004 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452666 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203005 !Sample_title = cy103_CD45pos_S238 !Sample_geo_accession = GSM3203005 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452665 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203006 !Sample_title = cy103_CD45pos_S209 !Sample_geo_accession = GSM3203006 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452664 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203007 !Sample_title = cy103_CD45pos_S228 !Sample_geo_accession = GSM3203007 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452663 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203008 !Sample_title = cy110_CD45pos_S291 !Sample_geo_accession = GSM3203008 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452662 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203009 !Sample_title = cy103_CD45pos_S227 !Sample_geo_accession = GSM3203009 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452661 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203010 !Sample_title = cy110_CD45neg_CD90neg_S114 !Sample_geo_accession = GSM3203010 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452660 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203011 !Sample_title = cy110_CD45pos_S354 !Sample_geo_accession = GSM3203011 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452659 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203012 !Sample_title = cy110_CD45pos_S300 !Sample_geo_accession = GSM3203012 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452658 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203013 !Sample_title = cy103_CD45neg_CD90neg_S79 !Sample_geo_accession = GSM3203013 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452657 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203014 !Sample_title = cy103_CD45neg_CD90neg_S88 !Sample_geo_accession = GSM3203014 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451662 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203015 !Sample_title = cy103_CD45neg_CD90neg_S38 !Sample_geo_accession = GSM3203015 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451661 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203016 !Sample_title = cy103_CD45pos_S275 !Sample_geo_accession = GSM3203016 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451660 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203017 !Sample_title = cy103_CD45pos_S244 !Sample_geo_accession = GSM3203017 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451659 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203018 !Sample_title = cy110_CD45neg_CD90neg_S110 !Sample_geo_accession = GSM3203018 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451658 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203019 !Sample_title = cy103_CD45pos_S276 !Sample_geo_accession = GSM3203019 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451657 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203020 !Sample_title = cy103_CD45neg_CD90neg_S9 !Sample_geo_accession = GSM3203020 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451656 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203021 !Sample_title = cy103_CD45neg_CD90neg_S61 !Sample_geo_accession = GSM3203021 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451655 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203022 !Sample_title = cy103_CD45pos_S235 !Sample_geo_accession = GSM3203022 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451723 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203023 !Sample_title = cy103_CD45neg_CD90neg_S23 !Sample_geo_accession = GSM3203023 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451722 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203024 !Sample_title = cy103_CD45pos_S252 !Sample_geo_accession = GSM3203024 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451721 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203025 !Sample_title = cy110_CD45pos_S296 !Sample_geo_accession = GSM3203025 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451720 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203026 !Sample_title = cy103_CD45neg_CD90neg_S78 !Sample_geo_accession = GSM3203026 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451719 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203027 !Sample_title = cy103_CD45pos_S258 !Sample_geo_accession = GSM3203027 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451718 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203028 !Sample_title = cy110_CD45pos_S317 !Sample_geo_accession = GSM3203028 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451717 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203029 !Sample_title = cy110_CD45pos_S375 !Sample_geo_accession = GSM3203029 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451716 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203030 !Sample_title = cy110_CD45pos_S307 !Sample_geo_accession = GSM3203030 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451715 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203031 !Sample_title = cy110_CD45neg_CD90neg_S153 !Sample_geo_accession = GSM3203031 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451714 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203032 !Sample_title = cy103_CD45pos_S251 !Sample_geo_accession = GSM3203032 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451713 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203033 !Sample_title = cy103_CD45neg_CD90neg_S95 !Sample_geo_accession = GSM3203033 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451706 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203034 !Sample_title = cy103_CD45pos_S274 !Sample_geo_accession = GSM3203034 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451712 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203035 !Sample_title = cy103_CD45neg_CD90neg_S22 !Sample_geo_accession = GSM3203035 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451711 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203036 !Sample_title = cy103_CD45neg_CD90neg_S51 !Sample_geo_accession = GSM3203036 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451710 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203037 !Sample_title = cy110_CD45pos_S299 !Sample_geo_accession = GSM3203037 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451709 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203038 !Sample_title = cy110_CD45pos_S314 !Sample_geo_accession = GSM3203038 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451708 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203039 !Sample_title = cy103_CD45pos_S223 !Sample_geo_accession = GSM3203039 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451707 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203040 !Sample_title = cy110_CD45neg_CD90neg_S129 !Sample_geo_accession = GSM3203040 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451705 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203041 !Sample_title = cy103_CD45pos_S231 !Sample_geo_accession = GSM3203041 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451704 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203042 !Sample_title = cy110_CD45pos_S316 !Sample_geo_accession = GSM3203042 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451703 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203043 !Sample_title = cy103_CD45neg_CD90neg_S76 !Sample_geo_accession = GSM3203043 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451702 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203044 !Sample_title = cy110_CD45neg_CD90neg_S182 !Sample_geo_accession = GSM3203044 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452656 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203045 !Sample_title = cy103_CD45pos_S226 !Sample_geo_accession = GSM3203045 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452655 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203046 !Sample_title = cy110_CD45pos_S334 !Sample_geo_accession = GSM3203046 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452654 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203047 !Sample_title = cy103_CD45neg_CD90neg_S80 !Sample_geo_accession = GSM3203047 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452653 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203048 !Sample_title = cy110_CD45neg_CD90neg_S160 !Sample_geo_accession = GSM3203048 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452652 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203049 !Sample_title = cy110_CD45pos_S373 !Sample_geo_accession = GSM3203049 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452651 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203050 !Sample_title = cy110_CD45neg_CD90neg_S152 !Sample_geo_accession = GSM3203050 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452650 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203051 !Sample_title = cy103_CD45pos_S218 !Sample_geo_accession = GSM3203051 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452649 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203052 !Sample_title = cy103_CD45pos_S242 !Sample_geo_accession = GSM3203052 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452648 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203053 !Sample_title = cy110_CD45neg_CD90neg_S121 !Sample_geo_accession = GSM3203053 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452647 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203054 !Sample_title = cy110_CD45pos_S294 !Sample_geo_accession = GSM3203054 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452646 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203055 !Sample_title = cy103_CD45pos_S233 !Sample_geo_accession = GSM3203055 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452645 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203056 !Sample_title = cy103_CD45neg_CD90neg_S43 !Sample_geo_accession = GSM3203056 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452644 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203057 !Sample_title = cy110_CD45neg_CD90neg_S162 !Sample_geo_accession = GSM3203057 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452643 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203058 !Sample_title = cy103_CD45pos_S203 !Sample_geo_accession = GSM3203058 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452642 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203059 !Sample_title = cy103_CD45neg_CD90neg_S63 !Sample_geo_accession = GSM3203059 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452641 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203060 !Sample_title = cy110_CD45neg_CD90neg_S165 !Sample_geo_accession = GSM3203060 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452715 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203061 !Sample_title = cy103_CD45pos_S239 !Sample_geo_accession = GSM3203061 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452714 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203062 !Sample_title = cy110_CD45neg_CD90neg_S168 !Sample_geo_accession = GSM3203062 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452713 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203063 !Sample_title = cy110_CD45neg_CD90neg_S139 !Sample_geo_accession = GSM3203063 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452712 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203064 !Sample_title = cy110_CD45pos_S293 !Sample_geo_accession = GSM3203064 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452718 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203065 !Sample_title = cy103_CD45pos_S234 !Sample_geo_accession = GSM3203065 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452717 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203066 !Sample_title = cy110_CD45neg_CD90neg_S106 !Sample_geo_accession = GSM3203066 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452716 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203067 !Sample_title = cy103_CD45pos_S279 !Sample_geo_accession = GSM3203067 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452711 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203068 !Sample_title = cy110_CD45neg_CD90neg_S138 !Sample_geo_accession = GSM3203068 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452710 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203069 !Sample_title = cy103_CD45pos_S283 !Sample_geo_accession = GSM3203069 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452709 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203070 !Sample_title = cy103_CD45neg_CD90neg_S69 !Sample_geo_accession = GSM3203070 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452708 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203071 !Sample_title = cy103_CD45pos_S217 !Sample_geo_accession = GSM3203071 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452707 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203072 !Sample_title = cy110_CD45pos_S328 !Sample_geo_accession = GSM3203072 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452706 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203073 !Sample_title = cy103_CD45neg_CD90neg_S73 !Sample_geo_accession = GSM3203073 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452705 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203074 !Sample_title = cy110_CD45pos_S338 !Sample_geo_accession = GSM3203074 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452787 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203075 !Sample_title = cy110_CD45neg_CD90neg_S157 !Sample_geo_accession = GSM3203075 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452786 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203076 !Sample_title = cy110_CD45pos_S325 !Sample_geo_accession = GSM3203076 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452785 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203077 !Sample_title = cy110_CD45pos_S374 !Sample_geo_accession = GSM3203077 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452854 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203078 !Sample_title = cy110_CD45pos_S382 !Sample_geo_accession = GSM3203078 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452853 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203079 !Sample_title = cy103_CD45pos_S205 !Sample_geo_accession = GSM3203079 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452852 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203080 !Sample_title = cy103_CD45pos_S272 !Sample_geo_accession = GSM3203080 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452851 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203081 !Sample_title = cy103_CD45pos_S246 !Sample_geo_accession = GSM3203081 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452850 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203082 !Sample_title = cy110_CD45pos_S289 !Sample_geo_accession = GSM3203082 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452849 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203083 !Sample_title = cy110_CD45pos_S298 !Sample_geo_accession = GSM3203083 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452848 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203084 !Sample_title = cy103_CD45pos_S236 !Sample_geo_accession = GSM3203084 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452847 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203085 !Sample_title = cy110_CD45pos_S318 !Sample_geo_accession = GSM3203085 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452846 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203086 !Sample_title = cy103_CD45pos_S241 !Sample_geo_accession = GSM3203086 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452845 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203087 !Sample_title = cy103_CD45neg_CD90neg_S72 !Sample_geo_accession = GSM3203087 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452844 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203088 !Sample_title = cy103_CD45pos_S197 !Sample_geo_accession = GSM3203088 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452843 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203089 !Sample_title = cy103_CD45pos_S219 !Sample_geo_accession = GSM3203089 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452842 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203090 !Sample_title = cy103_CD45pos_S277 !Sample_geo_accession = GSM3203090 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452841 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203091 !Sample_title = cy110_CD45pos_S355 !Sample_geo_accession = GSM3203091 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452840 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203092 !Sample_title = cy110_CD45neg_CD90neg_S161 !Sample_geo_accession = GSM3203092 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452839 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203093 !Sample_title = cy103_CD45neg_CD90neg_S56 !Sample_geo_accession = GSM3203093 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452838 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203094 !Sample_title = cy110_CD45pos_S379 !Sample_geo_accession = GSM3203094 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452837 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203095 !Sample_title = cy103_CD45pos_S256 !Sample_geo_accession = GSM3203095 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452836 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203096 !Sample_title = cy103_CD45neg_CD90neg_S42 !Sample_geo_accession = GSM3203096 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452835 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203097 !Sample_title = cy103_CD45neg_CD90neg_S82 !Sample_geo_accession = GSM3203097 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452834 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203098 !Sample_title = cy110_CD45pos_S366 !Sample_geo_accession = GSM3203098 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452833 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203099 !Sample_title = cy103_CD45neg_CD90neg_S3 !Sample_geo_accession = GSM3203099 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452832 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203100 !Sample_title = cy110_CD45neg_CD90neg_S98 !Sample_geo_accession = GSM3203100 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452831 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203101 !Sample_title = cy103_CD45neg_CD90neg_S14 !Sample_geo_accession = GSM3203101 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452830 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203102 !Sample_title = cy103_CD45neg_CD90neg_S19 !Sample_geo_accession = GSM3203102 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452829 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203103 !Sample_title = cy110_CD45neg_CD90neg_S166 !Sample_geo_accession = GSM3203103 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452828 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203104 !Sample_title = cy103_CD45pos_S266 !Sample_geo_accession = GSM3203104 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452906 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203105 !Sample_title = cy110_CD45pos_S302 !Sample_geo_accession = GSM3203105 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452905 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203106 !Sample_title = cy110_CD45pos_S310 !Sample_geo_accession = GSM3203106 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452904 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203107 !Sample_title = cy103_CD45pos_S213 !Sample_geo_accession = GSM3203107 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452903 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203108 !Sample_title = cy103_CD45neg_CD90neg_S33 !Sample_geo_accession = GSM3203108 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452902 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203109 !Sample_title = cy110_CD45pos_S330 !Sample_geo_accession = GSM3203109 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452901 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203110 !Sample_title = cy110_CD45pos_S376 !Sample_geo_accession = GSM3203110 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452900 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203111 !Sample_title = cy103_CD45pos_S200 !Sample_geo_accession = GSM3203111 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452899 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203112 !Sample_title = cy110_CD45neg_CD90neg_S140 !Sample_geo_accession = GSM3203112 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452898 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203113 !Sample_title = cy110_CD45neg_CD90neg_S169 !Sample_geo_accession = GSM3203113 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452897 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203114 !Sample_title = cy110_CD45neg_CD90neg_S107 !Sample_geo_accession = GSM3203114 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452896 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203115 !Sample_title = cy103_CD45neg_CD90neg_S75 !Sample_geo_accession = GSM3203115 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452895 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203116 !Sample_title = cy110_CD45neg_CD90neg_S118 !Sample_geo_accession = GSM3203116 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452894 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203117 !Sample_title = cy110_CD45pos_S351 !Sample_geo_accession = GSM3203117 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452893 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203118 !Sample_title = cy103_CD45neg_CD90neg_S4 !Sample_geo_accession = GSM3203118 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452892 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203119 !Sample_title = cy103_CD45neg_CD90neg_S47 !Sample_geo_accession = GSM3203119 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452957 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203120 !Sample_title = cy103_CD45neg_CD90neg_S89 !Sample_geo_accession = GSM3203120 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452956 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203121 !Sample_title = cy110_CD45pos_S323 !Sample_geo_accession = GSM3203121 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452955 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203122 !Sample_title = cy103_CD45pos_S216 !Sample_geo_accession = GSM3203122 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452954 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203123 !Sample_title = cy110_CD45neg_CD90neg_S163 !Sample_geo_accession = GSM3203123 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452953 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203124 !Sample_title = cy103_CD45pos_S254 !Sample_geo_accession = GSM3203124 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452952 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203125 !Sample_title = cy103_CD45neg_CD90neg_S26 !Sample_geo_accession = GSM3203125 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452951 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203126 !Sample_title = cy110_CD45pos_S309 !Sample_geo_accession = GSM3203126 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452950 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203127 !Sample_title = cy103_CD45pos_S280 !Sample_geo_accession = GSM3203127 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452949 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203128 !Sample_title = cy103_CD45pos_S271 !Sample_geo_accession = GSM3203128 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452948 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203129 !Sample_title = cy103_CD45pos_S214 !Sample_geo_accession = GSM3203129 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452947 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203130 !Sample_title = cy103_CD45neg_CD90neg_S90 !Sample_geo_accession = GSM3203130 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452946 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203131 !Sample_title = cy110_CD45neg_CD90neg_S189 !Sample_geo_accession = GSM3203131 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452945 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203132 !Sample_title = cy103_CD45pos_S262 !Sample_geo_accession = GSM3203132 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452944 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203133 !Sample_title = cy103_CD45neg_CD90neg_S37 !Sample_geo_accession = GSM3203133 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452943 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203134 !Sample_title = cy103_CD45pos_S201 !Sample_geo_accession = GSM3203134 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451701 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203135 !Sample_title = cy110_CD45pos_S357 !Sample_geo_accession = GSM3203135 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451700 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203136 !Sample_title = cy103_CD45neg_CD90neg_S36 !Sample_geo_accession = GSM3203136 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451699 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203137 !Sample_title = cy103_CD45pos_S243 !Sample_geo_accession = GSM3203137 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451698 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203138 !Sample_title = cy103_CD45neg_CD90neg_S32 !Sample_geo_accession = GSM3203138 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451697 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203139 !Sample_title = cy103_CD45pos_S194 !Sample_geo_accession = GSM3203139 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451696 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203140 !Sample_title = cy103_CD45pos_S232 !Sample_geo_accession = GSM3203140 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451695 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203141 !Sample_title = cy110_CD45pos_S337 !Sample_geo_accession = GSM3203141 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451694 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203142 !Sample_title = cy103_CD45pos_S198 !Sample_geo_accession = GSM3203142 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451693 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203143 !Sample_title = cy103_CD45pos_S259 !Sample_geo_accession = GSM3203143 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451692 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203144 !Sample_title = cy103_CD45neg_CD90neg_S39 !Sample_geo_accession = GSM3203144 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451691 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203145 !Sample_title = cy103_CD45pos_S257 !Sample_geo_accession = GSM3203145 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451690 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203146 !Sample_title = cy103_CD45pos_S286 !Sample_geo_accession = GSM3203146 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451689 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203147 !Sample_title = cy103_CD45neg_CD90neg_S15 !Sample_geo_accession = GSM3203147 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451761 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203148 !Sample_title = cy103_CD45pos_S221 !Sample_geo_accession = GSM3203148 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451760 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203149 !Sample_title = cy110_CD45pos_S362 !Sample_geo_accession = GSM3203149 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451759 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203150 !Sample_title = cy110_CD45pos_S345 !Sample_geo_accession = GSM3203150 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451758 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203151 !Sample_title = cy103_CD45neg_CD90neg_S70 !Sample_geo_accession = GSM3203151 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451752 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203152 !Sample_title = cy103_CD45neg_CD90neg_S44 !Sample_geo_accession = GSM3203152 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451751 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203153 !Sample_title = cy110_CD45neg_CD90neg_S167 !Sample_geo_accession = GSM3203153 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451750 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203154 !Sample_title = cy103_CD45pos_S270 !Sample_geo_accession = GSM3203154 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451757 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203155 !Sample_title = cy110_CD45neg_CD90neg_S150 !Sample_geo_accession = GSM3203155 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451756 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203156 !Sample_title = cy110_CD45pos_S333 !Sample_geo_accession = GSM3203156 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451755 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203157 !Sample_title = cy103_CD45pos_S215 !Sample_geo_accession = GSM3203157 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451754 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203158 !Sample_title = cy103_CD45neg_CD90neg_S50 !Sample_geo_accession = GSM3203158 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451753 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203159 !Sample_title = cy103_CD45pos_S212 !Sample_geo_accession = GSM3203159 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451749 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203160 !Sample_title = cy103_CD45neg_CD90neg_S71 !Sample_geo_accession = GSM3203160 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451748 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203161 !Sample_title = cy103_CD45pos_S193 !Sample_geo_accession = GSM3203161 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451747 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203162 !Sample_title = cy110_CD45neg_CD90neg_S103 !Sample_geo_accession = GSM3203162 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451746 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203163 !Sample_title = cy103_CD45pos_S206 !Sample_geo_accession = GSM3203163 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451745 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203164 !Sample_title = cy110_CD45pos_S350 !Sample_geo_accession = GSM3203164 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451744 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203165 !Sample_title = cy103_CD45neg_CD90neg_S85 !Sample_geo_accession = GSM3203165 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451743 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203166 !Sample_title = cy103_CD45pos_S281 !Sample_geo_accession = GSM3203166 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451742 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203167 !Sample_title = cy103_CD45pos_S207 !Sample_geo_accession = GSM3203167 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451741 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203168 !Sample_title = cy110_CD45pos_S315 !Sample_geo_accession = GSM3203168 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451740 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203169 !Sample_title = cy103_CD45neg_CD90neg_S21 !Sample_geo_accession = GSM3203169 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451739 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203170 !Sample_title = cy110_CD45neg_CD90neg_S149 !Sample_geo_accession = GSM3203170 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451738 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203171 !Sample_title = cy110_CD45neg_CD90neg_S145 !Sample_geo_accession = GSM3203171 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451737 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203172 !Sample_title = cy103_CD45neg_CD90neg_S55 !Sample_geo_accession = GSM3203172 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451736 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203173 !Sample_title = cy103_CD45neg_CD90neg_S66 !Sample_geo_accession = GSM3203173 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451735 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203174 !Sample_title = cy103_CD45neg_CD90neg_S54 !Sample_geo_accession = GSM3203174 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451734 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203175 !Sample_title = cy110_CD45pos_S347 !Sample_geo_accession = GSM3203175 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451733 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203176 !Sample_title = cy103_CD45pos_S240 !Sample_geo_accession = GSM3203176 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451732 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203177 !Sample_title = cy103_CD45neg_CD90neg_S46 !Sample_geo_accession = GSM3203177 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451731 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203178 !Sample_title = cy103_CD45neg_CD90neg_S8 !Sample_geo_accession = GSM3203178 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451730 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203179 !Sample_title = cy103_CD45pos_S255 !Sample_geo_accession = GSM3203179 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451727 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203180 !Sample_title = cy110_CD45pos_S371 !Sample_geo_accession = GSM3203180 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451726 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203181 !Sample_title = cy103_CD45neg_CD90neg_S81 !Sample_geo_accession = GSM3203181 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451725 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203182 !Sample_title = cy103_CD45pos_S237 !Sample_geo_accession = GSM3203182 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451724 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203183 !Sample_title = cy103_CD45neg_CD90neg_S62 !Sample_geo_accession = GSM3203183 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451799 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203184 !Sample_title = cy110_CD45neg_CD90neg_S147 !Sample_geo_accession = GSM3203184 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451729 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203185 !Sample_title = cy103_CD45neg_CD90neg_S67 !Sample_geo_accession = GSM3203185 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451728 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203186 !Sample_title = cy110_CD45pos_S322 !Sample_geo_accession = GSM3203186 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451798 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203187 !Sample_title = cy103_CD45neg_CD90neg_S31 !Sample_geo_accession = GSM3203187 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451797 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203188 !Sample_title = cy103_CD45neg_CD90neg_S12 !Sample_geo_accession = GSM3203188 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451796 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203189 !Sample_title = cy110_CD45pos_S369 !Sample_geo_accession = GSM3203189 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451795 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203190 !Sample_title = cy110_CD45pos_S311 !Sample_geo_accession = GSM3203190 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451794 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203191 !Sample_title = cy110_CD45neg_CD90neg_S180 !Sample_geo_accession = GSM3203191 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451793 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203192 !Sample_title = cy110_CD45pos_S377 !Sample_geo_accession = GSM3203192 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451792 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203193 !Sample_title = cy103_CD45pos_S208 !Sample_geo_accession = GSM3203193 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451791 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203194 !Sample_title = cy103_CD45pos_S211 !Sample_geo_accession = GSM3203194 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel103 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451790 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203195 !Sample_title = cy110_CD45pos_S295 !Sample_geo_accession = GSM3203195 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel110 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451789 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203196 !Sample_title = CY116CD45neg_CD90neg_S284 !Sample_geo_accession = GSM3203196 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451788 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203197 !Sample_title = CY116CD45neg_CD90neg_S196 !Sample_geo_accession = GSM3203197 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel116 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451787 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203198 !Sample_title = CY106_CD45neg_CD90neg_S341 !Sample_geo_accession = GSM3203198 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451786 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203199 !Sample_title = CY106_CD45neg_CD90neg_S291 !Sample_geo_accession = GSM3203199 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451785 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203200 !Sample_title = CY106_CD45neg_CD90neg_S301 !Sample_geo_accession = GSM3203200 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451784 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203201 !Sample_title = CY106_CD45neg_CD90neg_S378 !Sample_geo_accession = GSM3203201 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451783 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203202 !Sample_title = CY106_CD45neg_CD90neg_S310 !Sample_geo_accession = GSM3203202 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451782 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203203 !Sample_title = CY106_CD45neg_CD90neg_S354 !Sample_geo_accession = GSM3203203 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451781 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203204 !Sample_title = CY106_CD45neg_CD90neg_S292 !Sample_geo_accession = GSM3203204 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451780 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203205 !Sample_title = CY106_CD45neg_CD90neg_S302 !Sample_geo_accession = GSM3203205 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451779 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203206 !Sample_title = CY106_CD45neg_CD90neg_S294 !Sample_geo_accession = GSM3203206 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451778 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203207 !Sample_title = CY106_CD45neg_CD90neg_10cells_S289 !Sample_geo_accession = GSM3203207 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451777 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203208 !Sample_title = CY106_CD45neg_CD90neg_S374 !Sample_geo_accession = GSM3203208 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451776 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203209 !Sample_title = CY106_CD45neg_CD90neg_S382 !Sample_geo_accession = GSM3203209 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451775 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203210 !Sample_title = CY106_CD45neg_CD90neg_S326 !Sample_geo_accession = GSM3203210 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451774 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203211 !Sample_title = CY106_CD45neg_CD90neg_S297 !Sample_geo_accession = GSM3203211 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451773 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203212 !Sample_title = CY106_CD45neg_CD90neg_S356 !Sample_geo_accession = GSM3203212 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451772 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203213 !Sample_title = CY106_CD45neg_CD90neg_S328 !Sample_geo_accession = GSM3203213 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451771 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203214 !Sample_title = CY106_CD45neg_CD90neg_S338 !Sample_geo_accession = GSM3203214 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451770 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203215 !Sample_title = CY106_CD45neg_CD90neg_S323 !Sample_geo_accession = GSM3203215 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451769 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203216 !Sample_title = CY106_CD45neg_CD90neg_S308 !Sample_geo_accession = GSM3203216 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451768 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203217 !Sample_title = CY106_CD45neg_CD90neg_S344 !Sample_geo_accession = GSM3203217 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451767 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203218 !Sample_title = CY106_CD45neg_CD90neg_S365 !Sample_geo_accession = GSM3203218 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451766 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203219 !Sample_title = CY106_CD45neg_CD90neg_S320 !Sample_geo_accession = GSM3203219 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451765 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203220 !Sample_title = CY106_CD45neg_CD90neg_S327 !Sample_geo_accession = GSM3203220 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451764 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203221 !Sample_title = CY106_CD45neg_CD90neg_S366 !Sample_geo_accession = GSM3203221 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451763 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203222 !Sample_title = MGH00478_CD45pos_1_S211 !Sample_geo_accession = GSM3203222 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451762 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203223 !Sample_title = MGH00478_CD45pos_1_S193 !Sample_geo_accession = GSM3203223 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451836 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203224 !Sample_title = cy126_CD45pos_1_S16 !Sample_geo_accession = GSM3203224 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451835 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203225 !Sample_title = MGH00478_CD45pos_2_S353 !Sample_geo_accession = GSM3203225 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451834 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203226 !Sample_title = cy126_CD45pos_2_S123 !Sample_geo_accession = GSM3203226 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451833 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203227 !Sample_title = cy126_CD45pos_1_S1 !Sample_geo_accession = GSM3203227 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451832 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203228 !Sample_title = MGH00478_CD45pos_1_S286 !Sample_geo_accession = GSM3203228 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451831 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203229 !Sample_title = cy126_CD45pos_2_S155 !Sample_geo_accession = GSM3203229 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451830 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203230 !Sample_title = MGH00478_CD45pos_1_S257 !Sample_geo_accession = GSM3203230 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451829 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203231 !Sample_title = MGH00478_CD45pos_2_S377 !Sample_geo_accession = GSM3203231 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451828 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203232 !Sample_title = MGH00478_CD45pos_1_S279 !Sample_geo_accession = GSM3203232 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451827 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203233 !Sample_title = MGH00478_CD45pos_2_S327 !Sample_geo_accession = GSM3203233 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451826 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203234 !Sample_title = cy126_CD45pos_1_S88 !Sample_geo_accession = GSM3203234 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451825 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203235 !Sample_title = MGH00478_CD45pos_1_S195 !Sample_geo_accession = GSM3203235 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451824 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203236 !Sample_title = cy126_CD45pos_2_S134 !Sample_geo_accession = GSM3203236 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451823 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203237 !Sample_title = MGH00478_CD45pos_2_S363 !Sample_geo_accession = GSM3203237 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451822 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203238 !Sample_title = cy126_CD45pos_1_S79 !Sample_geo_accession = GSM3203238 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451821 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203239 !Sample_title = cy126_CD45pos_1_S8 !Sample_geo_accession = GSM3203239 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451820 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203240 !Sample_title = MGH00478_CD45pos_1_S210 !Sample_geo_accession = GSM3203240 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451819 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203241 !Sample_title = MGH00478_CD45pos_1_S215 !Sample_geo_accession = GSM3203241 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451818 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203242 !Sample_title = MGH00478_CD45pos_1_S203 !Sample_geo_accession = GSM3203242 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451813 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203243 !Sample_title = MGH00478_CD45pos_2_S313 !Sample_geo_accession = GSM3203243 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451812 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203244 !Sample_title = MGH00478_CD45pos_1_S234 !Sample_geo_accession = GSM3203244 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451817 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203245 !Sample_title = cy126_CD45pos_1_S10 !Sample_geo_accession = GSM3203245 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451816 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203246 !Sample_title = MGH00478_CD45pos_1_S282 !Sample_geo_accession = GSM3203246 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451815 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203247 !Sample_title = MGH00478_CD45pos_2_S319 !Sample_geo_accession = GSM3203247 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451814 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203248 !Sample_title = MGH00478_CD45pos_1_S229 !Sample_geo_accession = GSM3203248 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451811 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203249 !Sample_title = MGH00478_CD45pos_1_S232 !Sample_geo_accession = GSM3203249 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451810 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203250 !Sample_title = MGH00478_CD45pos_2_S292 !Sample_geo_accession = GSM3203250 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451809 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203251 !Sample_title = MGH00478_CD45pos_2_S316 !Sample_geo_accession = GSM3203251 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451808 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203252 !Sample_title = MGH00478_CD45pos_1_S230 !Sample_geo_accession = GSM3203252 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451807 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203253 !Sample_title = cy126_CD45pos_2_S117 !Sample_geo_accession = GSM3203253 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451806 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203254 !Sample_title = cy126_CD45pos_2_S181 !Sample_geo_accession = GSM3203254 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451805 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203255 !Sample_title = MGH00478_CD45pos_1_S214 !Sample_geo_accession = GSM3203255 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451804 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203256 !Sample_title = MGH00478_CD45pos_2_S376 !Sample_geo_accession = GSM3203256 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451803 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203257 !Sample_title = MGH00478_CD45pos_1_S225 !Sample_geo_accession = GSM3203257 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451802 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203258 !Sample_title = MGH00478_CD45pos_1_S216 !Sample_geo_accession = GSM3203258 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451801 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203259 !Sample_title = MGH00478_CD45pos_2_S356 !Sample_geo_accession = GSM3203259 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451800 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203260 !Sample_title = MGH00478_CD45pos_1_S244 !Sample_geo_accession = GSM3203260 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451871 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203261 !Sample_title = MGH00478_CD45pos_2_S289 !Sample_geo_accession = GSM3203261 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451870 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203262 !Sample_title = MGH00478_CD45pos_1_S287 !Sample_geo_accession = GSM3203262 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451869 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203263 !Sample_title = cy126_CD45pos_1_S22 !Sample_geo_accession = GSM3203263 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451868 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203264 !Sample_title = MGH00478_CD45pos_1_S251 !Sample_geo_accession = GSM3203264 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451867 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203265 !Sample_title = MGH00478_CD45pos_2_S324 !Sample_geo_accession = GSM3203265 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451866 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203266 !Sample_title = MGH00478_CD45pos_2_S351 !Sample_geo_accession = GSM3203266 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451865 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203267 !Sample_title = MGH00478_CD45pos_1_S263 !Sample_geo_accession = GSM3203267 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451864 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203268 !Sample_title = MGH00478_CD45pos_2_S379 !Sample_geo_accession = GSM3203268 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451863 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203269 !Sample_title = cy126_CD45pos_1_S89 !Sample_geo_accession = GSM3203269 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451862 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203270 !Sample_title = cy126_CD45pos_1_S21 !Sample_geo_accession = GSM3203270 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451861 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203271 !Sample_title = MGH00478_CD45pos_2_S320 !Sample_geo_accession = GSM3203271 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451860 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203272 !Sample_title = cy126_CD45pos_1_S30 !Sample_geo_accession = GSM3203272 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451859 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203273 !Sample_title = cy126_CD45pos_1_S66 !Sample_geo_accession = GSM3203273 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451858 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203274 !Sample_title = cy126_CD45pos_2_S164 !Sample_geo_accession = GSM3203274 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451857 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203275 !Sample_title = MGH00478_CD45pos_1_S259 !Sample_geo_accession = GSM3203275 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451856 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203276 !Sample_title = MGH00478_CD45pos_1_S201 !Sample_geo_accession = GSM3203276 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451855 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203277 !Sample_title = MGH00478_CD45pos_1_S231 !Sample_geo_accession = GSM3203277 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451854 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203278 !Sample_title = cy126_CD45pos_2_S172 !Sample_geo_accession = GSM3203278 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451853 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203279 !Sample_title = MGH00478_CD45pos_2_S368 !Sample_geo_accession = GSM3203279 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451852 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203280 !Sample_title = MGH00478_CD45pos_1_S260 !Sample_geo_accession = GSM3203280 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451851 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203281 !Sample_title = MGH00478_CD45pos_2_S362 !Sample_geo_accession = GSM3203281 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451850 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203282 !Sample_title = MGH00478_CD45pos_2_S293 !Sample_geo_accession = GSM3203282 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451849 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203283 !Sample_title = MGH00478_CD45pos_2_S291 !Sample_geo_accession = GSM3203283 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451848 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203284 !Sample_title = cy126_CD45pos_2_S158 !Sample_geo_accession = GSM3203284 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451847 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203285 !Sample_title = MGH00478_CD45pos_1_S256 !Sample_geo_accession = GSM3203285 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451846 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203286 !Sample_title = MGH00478_CD45pos_2_S328 !Sample_geo_accession = GSM3203286 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451845 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203287 !Sample_title = cy126_CD45pos_2_S173 !Sample_geo_accession = GSM3203287 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451844 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203288 !Sample_title = cy126_CD45pos_1_S78 !Sample_geo_accession = GSM3203288 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451843 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203289 !Sample_title = MGH00478_CD45pos_2_S312 !Sample_geo_accession = GSM3203289 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451842 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203290 !Sample_title = MGH00478_CD45pos_2_S332 !Sample_geo_accession = GSM3203290 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451841 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203291 !Sample_title = MGH00478_CD45pos_2_S344 !Sample_geo_accession = GSM3203291 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451840 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203292 !Sample_title = cy126_CD45pos_2_S152 !Sample_geo_accession = GSM3203292 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451839 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203293 !Sample_title = cy126_CD45pos_1_S28 !Sample_geo_accession = GSM3203293 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451838 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203294 !Sample_title = MGH00478_CD45pos_2_S369 !Sample_geo_accession = GSM3203294 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451837 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203295 !Sample_title = MGH00478_CD45pos_2_S371 !Sample_geo_accession = GSM3203295 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451906 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203296 !Sample_title = cy126_CD45pos_1_S87 !Sample_geo_accession = GSM3203296 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451905 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203297 !Sample_title = MGH00478_CD45pos_1_S284 !Sample_geo_accession = GSM3203297 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451904 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203298 !Sample_title = MGH00478_CD45pos_2_S339 !Sample_geo_accession = GSM3203298 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451903 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203299 !Sample_title = cy126_CD45pos_1_S3 !Sample_geo_accession = GSM3203299 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451902 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203300 !Sample_title = MGH00478_CD45pos_2_S373 !Sample_geo_accession = GSM3203300 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451901 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203301 !Sample_title = MGH00478_CD45pos_2_S374 !Sample_geo_accession = GSM3203301 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451900 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203302 !Sample_title = cy126_CD45pos_2_S114 !Sample_geo_accession = GSM3203302 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451899 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203303 !Sample_title = MGH00478_CD45pos_2_S350 !Sample_geo_accession = GSM3203303 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451898 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203304 !Sample_title = MGH00478_CD45pos_2_S383 !Sample_geo_accession = GSM3203304 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451897 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203305 !Sample_title = MGH00478_CD45pos_2_S341 !Sample_geo_accession = GSM3203305 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451896 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203306 !Sample_title = cy126_CD45pos_2_S130 !Sample_geo_accession = GSM3203306 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451895 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203307 !Sample_title = cy126_CD45pos_2_S137 !Sample_geo_accession = GSM3203307 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451894 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203308 !Sample_title = MGH00478_CD45pos_1_S202 !Sample_geo_accession = GSM3203308 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451893 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203309 !Sample_title = MGH00478_CD45pos_2_S340 !Sample_geo_accession = GSM3203309 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451892 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203310 !Sample_title = MGH00478_CD45pos_2_S337 !Sample_geo_accession = GSM3203310 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451891 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203311 !Sample_title = cy126_CD45pos_1_S27 !Sample_geo_accession = GSM3203311 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451890 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203312 !Sample_title = cy126_CD45pos_2_S180 !Sample_geo_accession = GSM3203312 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451889 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203313 !Sample_title = cy126_CD45pos_2_S175 !Sample_geo_accession = GSM3203313 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451888 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203314 !Sample_title = MGH00478_CD45pos_1_S270 !Sample_geo_accession = GSM3203314 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451887 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203315 !Sample_title = MGH00478_CD45pos_1_S218 !Sample_geo_accession = GSM3203315 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451886 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203316 !Sample_title = MGH00478_CD45pos_1_S196 !Sample_geo_accession = GSM3203316 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451885 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203317 !Sample_title = MGH00478_CD45pos_2_S360 !Sample_geo_accession = GSM3203317 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451884 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203318 !Sample_title = MGH00478_CD45pos_2_S343 !Sample_geo_accession = GSM3203318 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451883 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203319 !Sample_title = MGH00478_CD45pos_1_S253 !Sample_geo_accession = GSM3203319 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451882 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203320 !Sample_title = MGH00478_CD45pos_2_S301 !Sample_geo_accession = GSM3203320 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451881 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203321 !Sample_title = cy126_CD45pos_2_S166 !Sample_geo_accession = GSM3203321 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451880 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203322 !Sample_title = MGH00478_CD45pos_1_S219 !Sample_geo_accession = GSM3203322 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451879 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203323 !Sample_title = MGH00478_CD45pos_1_S248 !Sample_geo_accession = GSM3203323 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451878 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203324 !Sample_title = cy126_CD45pos_2_S177 !Sample_geo_accession = GSM3203324 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451877 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203325 !Sample_title = MGH00478_CD45pos_2_S347 !Sample_geo_accession = GSM3203325 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451876 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203326 !Sample_title = cy126_CD45pos_1_S57 !Sample_geo_accession = GSM3203326 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451875 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203327 !Sample_title = MGH00478_CD45pos_1_S264 !Sample_geo_accession = GSM3203327 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451874 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203328 !Sample_title = cy126_CD45pos_1_S54 !Sample_geo_accession = GSM3203328 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451873 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203329 !Sample_title = cy126_CD45pos_2_S190 !Sample_geo_accession = GSM3203329 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451941 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203330 !Sample_title = MGH00478_CD45pos_2_S308 !Sample_geo_accession = GSM3203330 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451940 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203331 !Sample_title = MGH00478_CD45pos_1_S205 !Sample_geo_accession = GSM3203331 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451939 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203332 !Sample_title = MGH00478_CD45pos_2_S310 !Sample_geo_accession = GSM3203332 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451938 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203333 !Sample_title = cy126_CD45pos_1_S31 !Sample_geo_accession = GSM3203333 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451937 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203334 !Sample_title = MGH00478_CD45pos_2_S354 !Sample_geo_accession = GSM3203334 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451872 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203335 !Sample_title = cy126_CD45pos_1_S39 !Sample_geo_accession = GSM3203335 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451936 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203336 !Sample_title = MGH00478_CD45pos_2_S349 !Sample_geo_accession = GSM3203336 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451935 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203337 !Sample_title = MGH00478_CD45pos_1_S206 !Sample_geo_accession = GSM3203337 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451934 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203338 !Sample_title = MGH00478_CD45pos_1_S200 !Sample_geo_accession = GSM3203338 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451933 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203339 !Sample_title = cy126_CD45pos_1_S6 !Sample_geo_accession = GSM3203339 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451932 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203340 !Sample_title = MGH00478_CD45pos_1_S268 !Sample_geo_accession = GSM3203340 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451931 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203341 !Sample_title = MGH00478_CD45pos_2_S370 !Sample_geo_accession = GSM3203341 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451930 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203342 !Sample_title = MGH00478_CD45pos_1_S261 !Sample_geo_accession = GSM3203342 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451929 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203343 !Sample_title = MGH00478_CD45pos_2_S364 !Sample_geo_accession = GSM3203343 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451928 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203344 !Sample_title = cy126_CD45pos_2_S185 !Sample_geo_accession = GSM3203344 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451927 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203345 !Sample_title = cy126_CD45pos_1_S62 !Sample_geo_accession = GSM3203345 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451926 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203346 !Sample_title = MGH00478_CD45pos_2_S380 !Sample_geo_accession = GSM3203346 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451925 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203347 !Sample_title = MGH00478_CD45pos_1_S224 !Sample_geo_accession = GSM3203347 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451924 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203348 !Sample_title = cy126_CD45pos_1_S53 !Sample_geo_accession = GSM3203348 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451923 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203349 !Sample_title = cy126_CD45pos_1_S25 !Sample_geo_accession = GSM3203349 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451922 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203350 !Sample_title = MGH00478_CD45pos_1_S194 !Sample_geo_accession = GSM3203350 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451921 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203351 !Sample_title = MGH00478_CD45pos_1_S245 !Sample_geo_accession = GSM3203351 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451920 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203352 !Sample_title = MGH00478_CD45pos_2_S330 !Sample_geo_accession = GSM3203352 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451919 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203353 !Sample_title = MGH00478_CD45pos_1_S249 !Sample_geo_accession = GSM3203353 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451918 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203354 !Sample_title = cy126_CD45pos_2_S132 !Sample_geo_accession = GSM3203354 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451917 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203355 !Sample_title = MGH00478_CD45pos_1_S265 !Sample_geo_accession = GSM3203355 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451916 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203356 !Sample_title = MGH00478_CD45pos_2_S315 !Sample_geo_accession = GSM3203356 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451915 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203357 !Sample_title = MGH00478_CD45pos_1_S227 !Sample_geo_accession = GSM3203357 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451914 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203358 !Sample_title = MGH00478_CD45pos_2_S338 !Sample_geo_accession = GSM3203358 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451913 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203359 !Sample_title = MGH00478_CD45pos_1_S212 !Sample_geo_accession = GSM3203359 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451912 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203360 !Sample_title = cy126_CD45pos_1_S75 !Sample_geo_accession = GSM3203360 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451911 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203361 !Sample_title = MGH00478_CD45pos_1_S252 !Sample_geo_accession = GSM3203361 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451910 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203362 !Sample_title = MGH00478_CD45pos_2_S381 !Sample_geo_accession = GSM3203362 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451909 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203363 !Sample_title = cy126_CD45pos_1_S86 !Sample_geo_accession = GSM3203363 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451908 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203364 !Sample_title = cy126_CD45pos_1_S49 !Sample_geo_accession = GSM3203364 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451907 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203365 !Sample_title = MGH00478_CD45pos_1_S258 !Sample_geo_accession = GSM3203365 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451979 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203366 !Sample_title = MGH00478_CD45pos_1_S226 !Sample_geo_accession = GSM3203366 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451978 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203367 !Sample_title = MGH00478_CD45pos_1_S223 !Sample_geo_accession = GSM3203367 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451977 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203368 !Sample_title = cy126_CD45pos_2_S183 !Sample_geo_accession = GSM3203368 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451976 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203369 !Sample_title = cy126_CD45pos_1_S56 !Sample_geo_accession = GSM3203369 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451975 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203370 !Sample_title = MGH00478_CD45pos_2_S302 !Sample_geo_accession = GSM3203370 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451974 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203371 !Sample_title = cy126_CD45pos_2_S191 !Sample_geo_accession = GSM3203371 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451973 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203372 !Sample_title = cy126_CD45pos_1_S23 !Sample_geo_accession = GSM3203372 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451972 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203373 !Sample_title = cy126_CD45pos_2_S135 !Sample_geo_accession = GSM3203373 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451971 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203374 !Sample_title = cy126_CD45pos_2_S97 !Sample_geo_accession = GSM3203374 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451970 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203375 !Sample_title = cy126_CD45pos_1_S93 !Sample_geo_accession = GSM3203375 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451969 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203376 !Sample_title = MGH00478_CD45pos_2_S358 !Sample_geo_accession = GSM3203376 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451968 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203377 !Sample_title = cy126_CD45pos_1_S67 !Sample_geo_accession = GSM3203377 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451967 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203378 !Sample_title = cy126_CD45pos_2_S139 !Sample_geo_accession = GSM3203378 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451966 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203379 !Sample_title = MGH00478_CD45pos_1_S272 !Sample_geo_accession = GSM3203379 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451965 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203380 !Sample_title = MGH00478_CD45pos_1_S278 !Sample_geo_accession = GSM3203380 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451964 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203381 !Sample_title = MGH00478_CD45pos_2_S322 !Sample_geo_accession = GSM3203381 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451963 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203382 !Sample_title = cy126_CD45pos_1_S90 !Sample_geo_accession = GSM3203382 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451962 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203383 !Sample_title = cy126_CD45pos_2_S148 !Sample_geo_accession = GSM3203383 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451961 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203384 !Sample_title = cy126_CD45pos_2_S119 !Sample_geo_accession = GSM3203384 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451960 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203385 !Sample_title = MGH00478_CD45pos_1_S237 !Sample_geo_accession = GSM3203385 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451959 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203386 !Sample_title = MGH00478_CD45pos_2_S345 !Sample_geo_accession = GSM3203386 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451958 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203387 !Sample_title = MGH00478_CD45pos_1_S285 !Sample_geo_accession = GSM3203387 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451957 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203388 !Sample_title = cy126_CD45pos_1_S59 !Sample_geo_accession = GSM3203388 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451956 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203389 !Sample_title = MGH00478_CD45pos_1_S255 !Sample_geo_accession = GSM3203389 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451953 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203390 !Sample_title = MGH00478_CD45pos_1_S197 !Sample_geo_accession = GSM3203390 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451952 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203391 !Sample_title = cy126_CD45pos_2_S100 !Sample_geo_accession = GSM3203391 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451951 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203392 !Sample_title = MGH00478_CD45pos_2_S306 !Sample_geo_accession = GSM3203392 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451950 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203393 !Sample_title = cy126_CD45pos_1_S70 !Sample_geo_accession = GSM3203393 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451949 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203394 !Sample_title = cy126_CD45pos_2_S108 !Sample_geo_accession = GSM3203394 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451955 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203395 !Sample_title = MGH00478_CD45pos_1_S208 !Sample_geo_accession = GSM3203395 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451954 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203396 !Sample_title = MGH00478_CD45pos_2_S359 !Sample_geo_accession = GSM3203396 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451948 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203397 !Sample_title = cy126_CD45pos_1_S81 !Sample_geo_accession = GSM3203397 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451947 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203398 !Sample_title = MGH00478_CD45pos_1_S209 !Sample_geo_accession = GSM3203398 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451946 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203399 !Sample_title = MGH00478_CD45pos_1_S274 !Sample_geo_accession = GSM3203399 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451945 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203400 !Sample_title = MGH00478_CD45pos_2_S372 !Sample_geo_accession = GSM3203400 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451944 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203401 !Sample_title = MGH00478_CD45pos_1_S238 !Sample_geo_accession = GSM3203401 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451943 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203402 !Sample_title = cy126_CD45pos_2_S178 !Sample_geo_accession = GSM3203402 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451942 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203403 !Sample_title = cy126_CD45pos_2_S112 !Sample_geo_accession = GSM3203403 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452013 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203404 !Sample_title = cy126_CD45pos_2_S184 !Sample_geo_accession = GSM3203404 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452012 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203405 !Sample_title = cy126_CD45pos_2_S182 !Sample_geo_accession = GSM3203405 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452011 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203406 !Sample_title = cy126_CD45pos_1_S17 !Sample_geo_accession = GSM3203406 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452010 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203407 !Sample_title = cy126_CD45pos_2_S144 !Sample_geo_accession = GSM3203407 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452009 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203408 !Sample_title = cy126_CD45pos_2_S160 !Sample_geo_accession = GSM3203408 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452008 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203409 !Sample_title = cy126_CD45pos_2_S187 !Sample_geo_accession = GSM3203409 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452007 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203410 !Sample_title = MGH00478_CD45pos_2_S303 !Sample_geo_accession = GSM3203410 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452006 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203411 !Sample_title = MGH00478_CD45pos_1_S213 !Sample_geo_accession = GSM3203411 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452005 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203412 !Sample_title = cy126_CD45pos_2_S174 !Sample_geo_accession = GSM3203412 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452004 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203413 !Sample_title = MGH00478_CD45pos_2_S361 !Sample_geo_accession = GSM3203413 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452003 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203414 !Sample_title = cy126_CD45pos_1_S92 !Sample_geo_accession = GSM3203414 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452002 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203415 !Sample_title = MGH00478_CD45pos_2_S307 !Sample_geo_accession = GSM3203415 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452001 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203416 !Sample_title = MGH00478_CD45pos_1_S266 !Sample_geo_accession = GSM3203416 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452000 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203417 !Sample_title = MGH00478_CD45pos_2_S326 !Sample_geo_accession = GSM3203417 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451999 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203418 !Sample_title = cy126_CD45pos_2_S170 !Sample_geo_accession = GSM3203418 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451998 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203419 !Sample_title = cy126_CD45pos_2_S120 !Sample_geo_accession = GSM3203419 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451997 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203420 !Sample_title = MGH00478_CD45pos_2_S329 !Sample_geo_accession = GSM3203420 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451996 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203421 !Sample_title = cy126_CD45pos_1_S36 !Sample_geo_accession = GSM3203421 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451995 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203422 !Sample_title = MGH00478_CD45pos_1_S276 !Sample_geo_accession = GSM3203422 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451994 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203423 !Sample_title = cy126_CD45pos_1_S48 !Sample_geo_accession = GSM3203423 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451993 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203424 !Sample_title = cy126_CD45pos_2_S176 !Sample_geo_accession = GSM3203424 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451992 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203425 !Sample_title = MGH00478_CD45pos_1_S243 !Sample_geo_accession = GSM3203425 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451991 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203426 !Sample_title = MGH00478_CD45pos_1_S262 !Sample_geo_accession = GSM3203426 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451990 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203427 !Sample_title = MGH00478_CD45pos_1_S198 !Sample_geo_accession = GSM3203427 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451989 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203428 !Sample_title = cy126_CD45pos_1_S35 !Sample_geo_accession = GSM3203428 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451988 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203429 !Sample_title = MGH00478_CD45pos_1_S221 !Sample_geo_accession = GSM3203429 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451987 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203430 !Sample_title = MGH00478_CD45pos_2_S382 !Sample_geo_accession = GSM3203430 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451986 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203431 !Sample_title = MGH00478_CD45pos_1_S277 !Sample_geo_accession = GSM3203431 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451985 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203432 !Sample_title = cy126_CD45pos_1_S65 !Sample_geo_accession = GSM3203432 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451984 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203433 !Sample_title = cy126_CD45pos_1_S77 !Sample_geo_accession = GSM3203433 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451983 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203434 !Sample_title = cy126_CD45pos_1_S12 !Sample_geo_accession = GSM3203434 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451982 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203435 !Sample_title = MGH00478_CD45pos_1_S241 !Sample_geo_accession = GSM3203435 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451981 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203436 !Sample_title = MGH00478_CD45pos_2_S346 !Sample_geo_accession = GSM3203436 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451980 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203437 !Sample_title = cy126_CD45pos_1_S24 !Sample_geo_accession = GSM3203437 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452051 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203438 !Sample_title = MGH00478_CD45pos_2_S348 !Sample_geo_accession = GSM3203438 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452050 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203439 !Sample_title = cy126_CD45pos_1_S80 !Sample_geo_accession = GSM3203439 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452049 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203440 !Sample_title = MGH00478_CD45pos_1_S217 !Sample_geo_accession = GSM3203440 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452048 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203441 !Sample_title = MGH00478_CD45pos_1_S199 !Sample_geo_accession = GSM3203441 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452047 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203442 !Sample_title = MGH00478_CD45pos_2_S309 !Sample_geo_accession = GSM3203442 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452046 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203443 !Sample_title = MGH00478_CD45pos_2_S367 !Sample_geo_accession = GSM3203443 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452045 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203444 !Sample_title = MGH00478_CD45pos_2_S336 !Sample_geo_accession = GSM3203444 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452044 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203445 !Sample_title = cy126_CD45pos_2_S129 !Sample_geo_accession = GSM3203445 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452043 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203446 !Sample_title = cy126_CD45pos_1_S46 !Sample_geo_accession = GSM3203446 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452042 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203447 !Sample_title = MGH00478_CD45pos_1_S273 !Sample_geo_accession = GSM3203447 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452041 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203448 !Sample_title = cy126_CD45pos_1_S58 !Sample_geo_accession = GSM3203448 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452040 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203449 !Sample_title = cy126_CD45pos_1_S33 !Sample_geo_accession = GSM3203449 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452039 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203450 !Sample_title = MGH00478_CD45pos_2_S375 !Sample_geo_accession = GSM3203450 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452038 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203451 !Sample_title = MGH00478_CD45pos_1_S236 !Sample_geo_accession = GSM3203451 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452037 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203452 !Sample_title = MGH00478_CD45pos_2_S334 !Sample_geo_accession = GSM3203452 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452036 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203453 !Sample_title = cy126_CD45pos_2_S169 !Sample_geo_accession = GSM3203453 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452035 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203454 !Sample_title = MGH00478_CD45pos_1_S254 !Sample_geo_accession = GSM3203454 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452034 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203455 !Sample_title = MGH00478_CD45pos_1_S269 !Sample_geo_accession = GSM3203455 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452033 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203456 !Sample_title = cy126_CD45pos_2_S127 !Sample_geo_accession = GSM3203456 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452032 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203457 !Sample_title = MGH00478_CD45pos_2_S357 !Sample_geo_accession = GSM3203457 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452031 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203458 !Sample_title = MGH00478_CD45pos_1_S204 !Sample_geo_accession = GSM3203458 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452030 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203459 !Sample_title = MGH00478_CD45pos_2_S335 !Sample_geo_accession = GSM3203459 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452029 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203460 !Sample_title = MGH00478_CD45pos_1_S233 !Sample_geo_accession = GSM3203460 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452028 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203461 !Sample_title = MGH00478_CD45pos_1_S228 !Sample_geo_accession = GSM3203461 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452027 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203462 !Sample_title = MGH00478_CD45pos_1_S267 !Sample_geo_accession = GSM3203462 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452026 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203463 !Sample_title = MGH00478_CD45pos_2_S305 !Sample_geo_accession = GSM3203463 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452025 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203464 !Sample_title = MGH00478_CD45pos_1_S283 !Sample_geo_accession = GSM3203464 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452024 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203465 !Sample_title = cy126_CD45pos_2_S149 !Sample_geo_accession = GSM3203465 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452023 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203466 !Sample_title = cy126_CD45pos_2_S153 !Sample_geo_accession = GSM3203466 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452022 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203467 !Sample_title = cy126_CD45pos_2_S159 !Sample_geo_accession = GSM3203467 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452021 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203468 !Sample_title = MGH00478_CD45pos_1_S242 !Sample_geo_accession = GSM3203468 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452020 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203469 !Sample_title = cy126_CD45pos_1_S18 !Sample_geo_accession = GSM3203469 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel126 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452019 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203470 !Sample_title = MGH00478_CD45pos_1_S250 !Sample_geo_accession = GSM3203470 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel478 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452018 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203471 !Sample_title = cy129PA_CD45pos_S13 !Sample_geo_accession = GSM3203471 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452017 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203472 !Sample_title = cy129PA_CD45pos_S70 !Sample_geo_accession = GSM3203472 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452016 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203473 !Sample_title = cy129PA_CD45pos_S20 !Sample_geo_accession = GSM3203473 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452015 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203474 !Sample_title = cy129PA_CD45neg_CD90neg_S163 !Sample_geo_accession = GSM3203474 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452014 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203475 !Sample_title = cy129PB_CD45pos_S264 !Sample_geo_accession = GSM3203475 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452086 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203476 !Sample_title = cy106_CD45neg_CD90neg_S363_19 !Sample_geo_accession = GSM3203476 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452085 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203477 !Sample_title = cy129PB_CD45pos_S213 !Sample_geo_accession = GSM3203477 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452084 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203478 !Sample_title = cy129PA_CD45pos_S59 !Sample_geo_accession = GSM3203478 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452083 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203479 !Sample_title = cy129PA_CD45pos_S24 !Sample_geo_accession = GSM3203479 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452082 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203480 !Sample_title = cy129PA_CD45pos_S11 !Sample_geo_accession = GSM3203480 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452081 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203481 !Sample_title = cy129PB_CD45pos_S261 !Sample_geo_accession = GSM3203481 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452077 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203482 !Sample_title = cy129PB_CD45pos_S288 !Sample_geo_accession = GSM3203482 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452076 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203483 !Sample_title = cy129PA_CD45pos_S15 !Sample_geo_accession = GSM3203483 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452075 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203484 !Sample_title = cy129PB_CD45pos_S231 !Sample_geo_accession = GSM3203484 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452080 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203485 !Sample_title = cy129PB_CD45pos_S247 !Sample_geo_accession = GSM3203485 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452079 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203486 !Sample_title = cy106_CD45neg_CD90neg_S309_20 !Sample_geo_accession = GSM3203486 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452078 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203487 !Sample_title = cy106_CD45neg_CD90neg_S378_21 !Sample_geo_accession = GSM3203487 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452074 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203488 !Sample_title = cy129PB_CD45pos_S202 !Sample_geo_accession = GSM3203488 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452073 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203489 !Sample_title = cy129PB_CD45pos_S208 !Sample_geo_accession = GSM3203489 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452072 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203490 !Sample_title = cy129PB_CD45pos_S199 !Sample_geo_accession = GSM3203490 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452071 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203491 !Sample_title = cy106_CD45neg_CD90neg_S320 !Sample_geo_accession = GSM3203491 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452070 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203492 !Sample_title = cy129PA_CD45pos_S6 !Sample_geo_accession = GSM3203492 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452069 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203493 !Sample_title = cy129PB_CD45pos_S258 !Sample_geo_accession = GSM3203493 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452068 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203494 !Sample_title = cy129PA_CD45pos_S71 !Sample_geo_accession = GSM3203494 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452067 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203495 !Sample_title = cy129PA_CD45pos_S1 !Sample_geo_accession = GSM3203495 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452066 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203496 !Sample_title = cy129PA_CD45pos_S83 !Sample_geo_accession = GSM3203496 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452065 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203497 !Sample_title = cy129PB_CD45pos_S250 !Sample_geo_accession = GSM3203497 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452064 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203498 !Sample_title = cy129PA_CD45neg_CD90neg_S183 !Sample_geo_accession = GSM3203498 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452063 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203499 !Sample_title = cy129PB_CD45pos_S201 !Sample_geo_accession = GSM3203499 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452062 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203500 !Sample_title = cy129PA_CD45pos_S91 !Sample_geo_accession = GSM3203500 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452061 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203501 !Sample_title = cy129PA_CD45neg_CD90neg_S139 !Sample_geo_accession = GSM3203501 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452060 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203502 !Sample_title = cy129PA_CD45neg_CD90neg_S172 !Sample_geo_accession = GSM3203502 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452059 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203503 !Sample_title = cy129PB_CD45pos_S265 !Sample_geo_accession = GSM3203503 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452058 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203504 !Sample_title = cy129PA_CD45neg_CD90neg_S110 !Sample_geo_accession = GSM3203504 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452057 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203505 !Sample_title = cy129PB_CD45pos_S222 !Sample_geo_accession = GSM3203505 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452056 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203506 !Sample_title = cy129PB_CD45pos_S253 !Sample_geo_accession = GSM3203506 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452055 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203507 !Sample_title = cy129PA_CD45neg_CD90neg_S105 !Sample_geo_accession = GSM3203507 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452054 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203508 !Sample_title = cy129PA_CD45pos_S2 !Sample_geo_accession = GSM3203508 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452053 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203509 !Sample_title = cy129PA_CD45neg_CD90neg_S108 !Sample_geo_accession = GSM3203509 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452052 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203510 !Sample_title = cy129PA_CD45pos_S84 !Sample_geo_accession = GSM3203510 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452122 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203511 !Sample_title = cy129PA_CD45pos_S26 !Sample_geo_accession = GSM3203511 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452121 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203512 !Sample_title = cy129PA_CD45neg_CD90neg_S99 !Sample_geo_accession = GSM3203512 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452120 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203513 !Sample_title = cy129PA_CD45neg_CD90neg_S189 !Sample_geo_accession = GSM3203513 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452119 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203514 !Sample_title = cy106_CD45neg_CD90neg_S347 !Sample_geo_accession = GSM3203514 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452118 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203515 !Sample_title = cy129PA_CD45neg_CD90neg_S140 !Sample_geo_accession = GSM3203515 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452117 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203516 !Sample_title = cy106_CD45neg_CD90neg_S311 !Sample_geo_accession = GSM3203516 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452116 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203517 !Sample_title = cy129PA_CD45pos_S77 !Sample_geo_accession = GSM3203517 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452115 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203518 !Sample_title = cy129PB_CD45pos_S230 !Sample_geo_accession = GSM3203518 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452114 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203519 !Sample_title = cy129PA_CD45pos_S86 !Sample_geo_accession = GSM3203519 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452113 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203520 !Sample_title = cy129PB_CD45pos_S194 !Sample_geo_accession = GSM3203520 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452112 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203521 !Sample_title = cy106_CD45neg_CD90neg_S366 !Sample_geo_accession = GSM3203521 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452111 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203522 !Sample_title = cy106_CD45neg_CD90neg_S348 !Sample_geo_accession = GSM3203522 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452110 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203523 !Sample_title = cy129PA_CD45neg_CD90neg_S154 !Sample_geo_accession = GSM3203523 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452109 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203524 !Sample_title = cy129PB_CD45pos_S211 !Sample_geo_accession = GSM3203524 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452108 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203525 !Sample_title = cy129PA_CD45neg_CD90neg_S169 !Sample_geo_accession = GSM3203525 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452107 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203526 !Sample_title = cy129PB_CD45pos_S274 !Sample_geo_accession = GSM3203526 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452106 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203527 !Sample_title = cy129PA_CD45neg_CD90neg_S127 !Sample_geo_accession = GSM3203527 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452105 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203528 !Sample_title = cy129PB_CD45pos_S226 !Sample_geo_accession = GSM3203528 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452104 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203529 !Sample_title = cy129PA_CD45neg_CD90neg_S175 !Sample_geo_accession = GSM3203529 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452103 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203530 !Sample_title = cy129PA_CD45neg_CD90neg_S151 !Sample_geo_accession = GSM3203530 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452102 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203531 !Sample_title = cy129PB_CD45pos_S269 !Sample_geo_accession = GSM3203531 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452101 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203532 !Sample_title = cy129PA_CD45pos_S79 !Sample_geo_accession = GSM3203532 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452100 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203533 !Sample_title = cy129PA_CD45neg_CD90neg_S171 !Sample_geo_accession = GSM3203533 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452099 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203534 !Sample_title = cy129PB_CD45pos_S232 !Sample_geo_accession = GSM3203534 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452098 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203535 !Sample_title = cy129PA_CD45neg_CD90neg_S120 !Sample_geo_accession = GSM3203535 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452097 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203536 !Sample_title = cy129PA_CD45pos_S34 !Sample_geo_accession = GSM3203536 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452096 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203537 !Sample_title = cy129PA_CD45neg_CD90neg_S167 !Sample_geo_accession = GSM3203537 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452095 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203538 !Sample_title = cy129PB_CD45pos_S275 !Sample_geo_accession = GSM3203538 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452094 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203539 !Sample_title = cy129PA_CD45neg_CD90neg_S132 !Sample_geo_accession = GSM3203539 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452093 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203540 !Sample_title = cy129PA_CD45pos_S67 !Sample_geo_accession = GSM3203540 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452091 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203541 !Sample_title = cy129PA_CD45pos_S23 !Sample_geo_accession = GSM3203541 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452090 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203542 !Sample_title = cy129PA_CD45pos_S68 !Sample_geo_accession = GSM3203542 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452089 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203543 !Sample_title = cy129PB_CD45pos_S206 !Sample_geo_accession = GSM3203543 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452088 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203544 !Sample_title = cy129PB_CD45pos_S229 !Sample_geo_accession = GSM3203544 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452092 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203545 !Sample_title = cy129PA_CD45neg_CD90neg_S128 !Sample_geo_accession = GSM3203545 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452087 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203546 !Sample_title = cy129PB_CD45pos_S193 !Sample_geo_accession = GSM3203546 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452159 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203547 !Sample_title = cy106_CD45neg_CD90neg_S356 !Sample_geo_accession = GSM3203547 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452158 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203548 !Sample_title = cy106_CD45neg_CD90neg_S327 !Sample_geo_accession = GSM3203548 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452157 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203549 !Sample_title = cy129PB_CD45pos_S223 !Sample_geo_accession = GSM3203549 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452156 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203550 !Sample_title = cy129PB_CD45pos_S263 !Sample_geo_accession = GSM3203550 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452155 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203551 !Sample_title = cy129PA_CD45pos_S29 !Sample_geo_accession = GSM3203551 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452154 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203552 !Sample_title = cy129PB_CD45pos_S233 !Sample_geo_accession = GSM3203552 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452153 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203553 !Sample_title = cy129PA_CD45neg_CD90neg_S137 !Sample_geo_accession = GSM3203553 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452152 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203554 !Sample_title = cy129PA_CD45neg_CD90neg_S190 !Sample_geo_accession = GSM3203554 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452151 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203555 !Sample_title = cy106_CD45neg_CD90neg_S337 !Sample_geo_accession = GSM3203555 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452150 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203556 !Sample_title = cy129PA_CD45neg_CD90neg_S98 !Sample_geo_accession = GSM3203556 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452149 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203557 !Sample_title = cy129PB_CD45pos_S236 !Sample_geo_accession = GSM3203557 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452148 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203558 !Sample_title = cy129PA_CD45pos_S18 !Sample_geo_accession = GSM3203558 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452147 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203559 !Sample_title = cy129PA_CD45neg_CD90neg_S122 !Sample_geo_accession = GSM3203559 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452146 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203560 !Sample_title = cy106_CD45neg_CD90neg_S319 !Sample_geo_accession = GSM3203560 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452145 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203561 !Sample_title = cy129PB_CD45pos_S286 !Sample_geo_accession = GSM3203561 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452144 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203562 !Sample_title = cy129PA_CD45neg_CD90neg_S125 !Sample_geo_accession = GSM3203562 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452143 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203563 !Sample_title = cy129PB_CD45pos_S240 !Sample_geo_accession = GSM3203563 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452142 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203564 !Sample_title = cy129PA_CD45neg_CD90neg_S161 !Sample_geo_accession = GSM3203564 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452141 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203565 !Sample_title = cy129PB_CD45pos_S273 !Sample_geo_accession = GSM3203565 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452140 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203566 !Sample_title = cy129PA_CD45pos_S25 !Sample_geo_accession = GSM3203566 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452139 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203567 !Sample_title = cy129PA_CD45pos_S40 !Sample_geo_accession = GSM3203567 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452138 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203568 !Sample_title = cy129PA_CD45pos_S5 !Sample_geo_accession = GSM3203568 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452137 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203569 !Sample_title = cy129PA_CD45neg_CD90neg_S129 !Sample_geo_accession = GSM3203569 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452136 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203570 !Sample_title = cy129PA_CD45pos_S65 !Sample_geo_accession = GSM3203570 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452135 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203571 !Sample_title = cy129PA_CD45neg_CD90neg_S138 !Sample_geo_accession = GSM3203571 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452134 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203572 !Sample_title = cy129PA_CD45neg_CD90neg_S142 !Sample_geo_accession = GSM3203572 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452133 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203573 !Sample_title = cy129PB_CD45pos_S246 !Sample_geo_accession = GSM3203573 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452132 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203574 !Sample_title = cy129PA_CD45neg_CD90neg_S149 !Sample_geo_accession = GSM3203574 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452131 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203575 !Sample_title = cy129PA_CD45neg_CD90neg_S134 !Sample_geo_accession = GSM3203575 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452130 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203576 !Sample_title = cy129PB_CD45pos_S276 !Sample_geo_accession = GSM3203576 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452129 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203577 !Sample_title = cy129PA_CD45pos_S16 !Sample_geo_accession = GSM3203577 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452128 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203578 !Sample_title = cy129PB_CD45pos_S252 !Sample_geo_accession = GSM3203578 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452127 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203579 !Sample_title = cy129PA_CD45pos_S75 !Sample_geo_accession = GSM3203579 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452126 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203580 !Sample_title = cy106_CD45neg_CD90neg_S289_22 !Sample_geo_accession = GSM3203580 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452125 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203581 !Sample_title = cy129PA_CD45neg_CD90neg_S150 !Sample_geo_accession = GSM3203581 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452124 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203582 !Sample_title = cy129PA_CD45pos_S95 !Sample_geo_accession = GSM3203582 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452123 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203583 !Sample_title = cy129PB_CD45pos_S203 !Sample_geo_accession = GSM3203583 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452198 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203584 !Sample_title = cy129PA_CD45pos_S85 !Sample_geo_accession = GSM3203584 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452197 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203585 !Sample_title = cy129PA_CD45pos_S52 !Sample_geo_accession = GSM3203585 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452196 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203586 !Sample_title = cy129PB_CD45pos_S207 !Sample_geo_accession = GSM3203586 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452195 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203587 !Sample_title = cy129PA_CD45pos_S89 !Sample_geo_accession = GSM3203587 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452194 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203588 !Sample_title = cy129PB_CD45pos_S243 !Sample_geo_accession = GSM3203588 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452193 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203589 !Sample_title = cy129PA_CD45pos_S45 !Sample_geo_accession = GSM3203589 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452192 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203590 !Sample_title = cy129PA_CD45pos_S54 !Sample_geo_accession = GSM3203590 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452191 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203591 !Sample_title = cy129PA_CD45pos_S47 !Sample_geo_accession = GSM3203591 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452190 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203592 !Sample_title = cy129PA_CD45pos_S3 !Sample_geo_accession = GSM3203592 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452189 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203593 !Sample_title = cy129PA_CD45neg_CD90neg_S130 !Sample_geo_accession = GSM3203593 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452188 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203594 !Sample_title = cy129PB_CD45pos_S245 !Sample_geo_accession = GSM3203594 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452187 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203595 !Sample_title = cy129PA_CD45neg_CD90neg_S186 !Sample_geo_accession = GSM3203595 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452186 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203596 !Sample_title = cy106_CD45neg_CD90neg_S290 !Sample_geo_accession = GSM3203596 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452185 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203597 !Sample_title = cy129PA_CD45pos_S14 !Sample_geo_accession = GSM3203597 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452184 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203598 !Sample_title = cy106_CD45neg_CD90neg_S294 !Sample_geo_accession = GSM3203598 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452183 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203599 !Sample_title = cy129PB_CD45pos_S254 !Sample_geo_accession = GSM3203599 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452182 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203600 !Sample_title = cy106_CD45neg_CD90neg_S349 !Sample_geo_accession = GSM3203600 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452181 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203601 !Sample_title = cy129PB_CD45pos_S200 !Sample_geo_accession = GSM3203601 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452180 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203602 !Sample_title = cy129PA_CD45neg_CD90neg_S153 !Sample_geo_accession = GSM3203602 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452179 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203603 !Sample_title = cy129PB_CD45pos_S268 !Sample_geo_accession = GSM3203603 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452178 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203604 !Sample_title = cy129PB_CD45pos_S279 !Sample_geo_accession = GSM3203604 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452177 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203605 !Sample_title = cy129PB_CD45pos_S285 !Sample_geo_accession = GSM3203605 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452176 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203606 !Sample_title = cy129PB_CD45pos_S242 !Sample_geo_accession = GSM3203606 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452175 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203607 !Sample_title = cy129PA_CD45pos_S44 !Sample_geo_accession = GSM3203607 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452174 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203608 !Sample_title = cy106_CD45neg_CD90neg_S338 !Sample_geo_accession = GSM3203608 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452173 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203609 !Sample_title = cy129PA_CD45pos_S55 !Sample_geo_accession = GSM3203609 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452172 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203610 !Sample_title = cy129PA_CD45pos_S94 !Sample_geo_accession = GSM3203610 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452171 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203611 !Sample_title = cy129PA_CD45pos_S69 !Sample_geo_accession = GSM3203611 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452170 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203612 !Sample_title = cy129PA_CD45neg_CD90neg_S191 !Sample_geo_accession = GSM3203612 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452169 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203613 !Sample_title = cy129PA_CD45pos_S51 !Sample_geo_accession = GSM3203613 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452168 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203614 !Sample_title = cy129PB_CD45pos_S209 !Sample_geo_accession = GSM3203614 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452167 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203615 !Sample_title = cy129PB_CD45pos_S280 !Sample_geo_accession = GSM3203615 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452166 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203616 !Sample_title = cy129PA_CD45pos_S4 !Sample_geo_accession = GSM3203616 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452165 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203617 !Sample_title = cy129PA_CD45pos_S88 !Sample_geo_accession = GSM3203617 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452164 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203618 !Sample_title = cy129PA_CD45pos_S58 !Sample_geo_accession = GSM3203618 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452163 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203619 !Sample_title = cy129PA_CD45pos_S28 !Sample_geo_accession = GSM3203619 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452162 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203620 !Sample_title = cy129PA_CD45pos_S19 !Sample_geo_accession = GSM3203620 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452161 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203621 !Sample_title = cy129PA_CD45pos_S9 !Sample_geo_accession = GSM3203621 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452160 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203622 !Sample_title = cy129PA_CD45pos_S36 !Sample_geo_accession = GSM3203622 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452224 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203623 !Sample_title = cy129PB_CD45pos_S204 !Sample_geo_accession = GSM3203623 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452223 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203624 !Sample_title = cy129PA_CD45neg_CD90neg_S126 !Sample_geo_accession = GSM3203624 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452222 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203625 !Sample_title = cy129PA_CD45pos_S76 !Sample_geo_accession = GSM3203625 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452221 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203626 !Sample_title = cy129PA_CD45neg_CD90neg_S144 !Sample_geo_accession = GSM3203626 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452220 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203627 !Sample_title = cy129PA_CD45neg_CD90neg_S97 !Sample_geo_accession = GSM3203627 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452219 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203628 !Sample_title = cy106_CD45neg_CD90neg_S365_23 !Sample_geo_accession = GSM3203628 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452218 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203629 !Sample_title = cy106_CD45neg_CD90neg_S373 !Sample_geo_accession = GSM3203629 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452217 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203630 !Sample_title = cy129PB_CD45pos_S266 !Sample_geo_accession = GSM3203630 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452216 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203631 !Sample_title = cy129PA_CD45neg_CD90neg_S164 !Sample_geo_accession = GSM3203631 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452215 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203632 !Sample_title = cy106_CD45neg_CD90neg_S364 !Sample_geo_accession = GSM3203632 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452214 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203633 !Sample_title = cy129PA_CD45neg_CD90neg_S114 !Sample_geo_accession = GSM3203633 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452213 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203634 !Sample_title = cy106_CD45neg_CD90neg_S357_24 !Sample_geo_accession = GSM3203634 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452212 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203635 !Sample_title = cy129PA_CD45pos_S35 !Sample_geo_accession = GSM3203635 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452211 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203636 !Sample_title = cy129PA_CD45pos_S87 !Sample_geo_accession = GSM3203636 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452210 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203637 !Sample_title = cy129PA_CD45pos_S49 !Sample_geo_accession = GSM3203637 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452209 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203638 !Sample_title = cy129PB_CD45pos_S281 !Sample_geo_accession = GSM3203638 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452208 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203639 !Sample_title = cy129PB_CD45pos_S215 !Sample_geo_accession = GSM3203639 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452207 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203640 !Sample_title = cy129PA_CD45neg_CD90neg_S182 !Sample_geo_accession = GSM3203640 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452206 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203641 !Sample_title = cy129PA_CD45neg_CD90neg_S107 !Sample_geo_accession = GSM3203641 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452205 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203642 !Sample_title = cy129PA_CD45pos_S41 !Sample_geo_accession = GSM3203642 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452204 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203643 !Sample_title = cy129PA_CD45pos_S57 !Sample_geo_accession = GSM3203643 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452203 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203644 !Sample_title = cy129PA_CD45neg_CD90neg_S162 !Sample_geo_accession = GSM3203644 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452202 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203645 !Sample_title = cy106_CD45neg_CD90neg_S299 !Sample_geo_accession = GSM3203645 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452201 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203646 !Sample_title = cy129PA_CD45neg_CD90neg_S141 !Sample_geo_accession = GSM3203646 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: CAF !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452200 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203647 !Sample_title = cy129PB_CD45pos_S282 !Sample_geo_accession = GSM3203647 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452199 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203648 !Sample_title = cy129PB_CD45pos_S214 !Sample_geo_accession = GSM3203648 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452258 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203649 !Sample_title = cy129PA_CD45neg_CD90neg_S136 !Sample_geo_accession = GSM3203649 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452257 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203650 !Sample_title = cy129PB_CD45pos_S277 !Sample_geo_accession = GSM3203650 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452256 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203651 !Sample_title = cy129PA_CD45neg_CD90neg_S103 !Sample_geo_accession = GSM3203651 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452255 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203652 !Sample_title = cy129PA_CD45pos_S43 !Sample_geo_accession = GSM3203652 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452254 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203653 !Sample_title = cy106_CD45neg_CD90neg_S325 !Sample_geo_accession = GSM3203653 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452253 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203654 !Sample_title = cy129PA_CD45pos_S82 !Sample_geo_accession = GSM3203654 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452252 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203655 !Sample_title = cy129PA_CD45neg_CD90neg_S123 !Sample_geo_accession = GSM3203655 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452251 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203656 !Sample_title = cy129PA_CD45pos_S22 !Sample_geo_accession = GSM3203656 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452250 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203657 !Sample_title = cy129PA_CD45pos_S12 !Sample_geo_accession = GSM3203657 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452249 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203658 !Sample_title = cy129PA_CD45pos_S39 !Sample_geo_accession = GSM3203658 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452248 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203659 !Sample_title = cy129PA_CD45neg_CD90neg_S174 !Sample_geo_accession = GSM3203659 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452247 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203660 !Sample_title = cy129PA_CD45neg_CD90neg_S106 !Sample_geo_accession = GSM3203660 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452246 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203661 !Sample_title = cy129PB_CD45pos_S221 !Sample_geo_accession = GSM3203661 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452245 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203662 !Sample_title = cy129PA_CD45neg_CD90neg_S173 !Sample_geo_accession = GSM3203662 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452244 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203663 !Sample_title = cy129PA_CD45pos_S92 !Sample_geo_accession = GSM3203663 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452243 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203664 !Sample_title = cy129PA_CD45neg_CD90neg_S181 !Sample_geo_accession = GSM3203664 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452242 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203665 !Sample_title = cy129PB_CD45pos_S278 !Sample_geo_accession = GSM3203665 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452241 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203666 !Sample_title = cy106_CD45neg_CD90neg_S344_25 !Sample_geo_accession = GSM3203666 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452240 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203667 !Sample_title = cy129PA_CD45pos_S66 !Sample_geo_accession = GSM3203667 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452239 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203668 !Sample_title = cy106_CD45neg_CD90neg_S306_26 !Sample_geo_accession = GSM3203668 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452238 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203669 !Sample_title = cy129PA_CD45neg_CD90neg_S118 !Sample_geo_accession = GSM3203669 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452237 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203670 !Sample_title = cy129PA_CD45pos_S60 !Sample_geo_accession = GSM3203670 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452236 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203671 !Sample_title = cy129PA_CD45pos_S30 !Sample_geo_accession = GSM3203671 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452235 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203672 !Sample_title = cy129PB_CD45pos_S239 !Sample_geo_accession = GSM3203672 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452234 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203673 !Sample_title = cy129PA_CD45neg_CD90neg_S184 !Sample_geo_accession = GSM3203673 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452233 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203674 !Sample_title = cy106_CD45neg_CD90neg_S291 !Sample_geo_accession = GSM3203674 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452232 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203675 !Sample_title = cy106_CD45neg_CD90neg_S352 !Sample_geo_accession = GSM3203675 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452231 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203676 !Sample_title = cy129PA_CD45pos_S53 !Sample_geo_accession = GSM3203676 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452230 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203677 !Sample_title = cy129PA_CD45pos_S64 !Sample_geo_accession = GSM3203677 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452229 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203678 !Sample_title = cy129PB_CD45pos_S237 !Sample_geo_accession = GSM3203678 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452228 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203679 !Sample_title = cy129PB_CD45pos_S270 !Sample_geo_accession = GSM3203679 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452227 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203680 !Sample_title = cy129PB_CD45pos_S234 !Sample_geo_accession = GSM3203680 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452226 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203681 !Sample_title = cy129PB_CD45pos_S196 !Sample_geo_accession = GSM3203681 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452225 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203682 !Sample_title = cy129PA_CD45neg_CD90neg_S119 !Sample_geo_accession = GSM3203682 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452299 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203683 !Sample_title = cy106_CD45neg_CD90neg_S376 !Sample_geo_accession = GSM3203683 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel106 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452298 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203684 !Sample_title = cy129PA_CD45neg_CD90neg_S157 !Sample_geo_accession = GSM3203684 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452297 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203685 !Sample_title = cy129PA_CD45pos_S27 !Sample_geo_accession = GSM3203685 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452296 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203686 !Sample_title = cy129PB_CD45pos_S284 !Sample_geo_accession = GSM3203686 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452295 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203687 !Sample_title = cy129PB_CD45pos_S224 !Sample_geo_accession = GSM3203687 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pb !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452294 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203688 !Sample_title = cy129PA_CD45pos_S31 !Sample_geo_accession = GSM3203688 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452293 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203689 !Sample_title = cy04.3_CD45pos_S4 !Sample_geo_accession = GSM3203689 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452292 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203690 !Sample_title = cy04.3_CD45pos_S12 !Sample_geo_accession = GSM3203690 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452291 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203691 !Sample_title = cy04.3_CD45pos_S16 !Sample_geo_accession = GSM3203691 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452286 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203692 !Sample_title = cy04.3_CD45pos_S56 !Sample_geo_accession = GSM3203692 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452290 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203693 !Sample_title = cy04.3_CD45pos_S8 !Sample_geo_accession = GSM3203693 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452289 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203694 !Sample_title = cy121.1_CD45pos_S260 !Sample_geo_accession = GSM3203694 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452288 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203695 !Sample_title = cy04.3_CD45pos_S52 !Sample_geo_accession = GSM3203695 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452287 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203696 !Sample_title = cy121.1_CD45pos_S230 !Sample_geo_accession = GSM3203696 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452285 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203697 !Sample_title = cy04.3_CD45pos_S70 !Sample_geo_accession = GSM3203697 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452284 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203698 !Sample_title = cy04.3_CD45pos_S81 !Sample_geo_accession = GSM3203698 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452283 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203699 !Sample_title = cy04.3_CD45pos_S45 !Sample_geo_accession = GSM3203699 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452282 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203700 !Sample_title = cy04.3_CD45pos_S33 !Sample_geo_accession = GSM3203700 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452281 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203701 !Sample_title = cy04.3_CD45pos_S62 !Sample_geo_accession = GSM3203701 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452280 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203702 !Sample_title = cy04.3_CD45pos_S69 !Sample_geo_accession = GSM3203702 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452279 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203703 !Sample_title = cy04.3_CD45pos_S9 !Sample_geo_accession = GSM3203703 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452278 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203704 !Sample_title = cy04.3_CD45pos_S47 !Sample_geo_accession = GSM3203704 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452277 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203705 !Sample_title = cy04.3_CD45pos_S60 !Sample_geo_accession = GSM3203705 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452276 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203706 !Sample_title = cy121.1_CD45pos_S216 !Sample_geo_accession = GSM3203706 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452275 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203707 !Sample_title = cy04.3_CD45pos_S65 !Sample_geo_accession = GSM3203707 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452274 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203708 !Sample_title = cy121.1_CD45pos_S204 !Sample_geo_accession = GSM3203708 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452273 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203709 !Sample_title = cy04.3_CD45pos_S14 !Sample_geo_accession = GSM3203709 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452272 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203710 !Sample_title = cy04.3_CD45pos_S32 !Sample_geo_accession = GSM3203710 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452271 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203711 !Sample_title = cy04.3_CD45pos_S6 !Sample_geo_accession = GSM3203711 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452270 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203712 !Sample_title = cy04.3_CD45pos_S75 !Sample_geo_accession = GSM3203712 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452269 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203713 !Sample_title = cy04.3_CD45pos_S86 !Sample_geo_accession = GSM3203713 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452268 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203714 !Sample_title = cy121.1_CD45pos_S250 !Sample_geo_accession = GSM3203714 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452267 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203715 !Sample_title = cy04.3_CD45pos_S31 !Sample_geo_accession = GSM3203715 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452266 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203716 !Sample_title = cy121.1_CD45neg_S289 !Sample_geo_accession = GSM3203716 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452261 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203717 !Sample_title = cy04.3_CD45pos_S59 !Sample_geo_accession = GSM3203717 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452265 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203718 !Sample_title = cy04.3_CD45pos_S66 !Sample_geo_accession = GSM3203718 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452264 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203719 !Sample_title = cy121.1_CD45pos_S232 !Sample_geo_accession = GSM3203719 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452263 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203720 !Sample_title = cy04.3_CD45pos_S17 !Sample_geo_accession = GSM3203720 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452262 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203721 !Sample_title = cy121.1_CD45pos_S215 !Sample_geo_accession = GSM3203721 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452260 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203722 !Sample_title = cy04.3_CD45pos_S40 !Sample_geo_accession = GSM3203722 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452259 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203723 !Sample_title = cy04.3_CD45pos_S55 !Sample_geo_accession = GSM3203723 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452333 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203724 !Sample_title = cy121.1_CD45pos_S200 !Sample_geo_accession = GSM3203724 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452332 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203725 !Sample_title = cy04.3_CD45pos_S21 !Sample_geo_accession = GSM3203725 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452331 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203726 !Sample_title = cy121.1_CD45pos_S271 !Sample_geo_accession = GSM3203726 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452330 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203727 !Sample_title = cy04.3_CD45pos_S36 !Sample_geo_accession = GSM3203727 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452329 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203728 !Sample_title = cy04.3_CD45pos_S28 !Sample_geo_accession = GSM3203728 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452328 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203729 !Sample_title = cy04.3_CD45pos_S89 !Sample_geo_accession = GSM3203729 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452327 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203730 !Sample_title = cy121.1_CD45pos_S236 !Sample_geo_accession = GSM3203730 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452326 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203731 !Sample_title = cy04.3_CD45pos_S37 !Sample_geo_accession = GSM3203731 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452325 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203732 !Sample_title = cy04.3_CD45pos_S1 !Sample_geo_accession = GSM3203732 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450378 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203733 !Sample_title = cy04.3_CD45pos_S57 !Sample_geo_accession = GSM3203733 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450377 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203734 !Sample_title = cy04.3_CD45pos_S7 !Sample_geo_accession = GSM3203734 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450413 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203735 !Sample_title = cy04.3_CD45pos_S20 !Sample_geo_accession = GSM3203735 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450412 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203736 !Sample_title = cy121.1_CD45pos_S196 !Sample_geo_accession = GSM3203736 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450411 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203737 !Sample_title = cy121.1_CD45pos_S195 !Sample_geo_accession = GSM3203737 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450410 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203738 !Sample_title = cy04.3_CD45pos_S39 !Sample_geo_accession = GSM3203738 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450409 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203739 !Sample_title = cy121.1_CD45pos_S248 !Sample_geo_accession = GSM3203739 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450408 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203740 !Sample_title = cy04.3_CD45pos_S72 !Sample_geo_accession = GSM3203740 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450407 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203741 !Sample_title = cy04.3_CD45pos_S82 !Sample_geo_accession = GSM3203741 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450406 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203742 !Sample_title = cy121.1_CD45pos_S235 !Sample_geo_accession = GSM3203742 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450405 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203743 !Sample_title = cy04.3_CD45pos_S22 !Sample_geo_accession = GSM3203743 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450404 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203744 !Sample_title = cy04.3_CD45pos_S27 !Sample_geo_accession = GSM3203744 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450403 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203745 !Sample_title = cy04.3_CD45pos_S26 !Sample_geo_accession = GSM3203745 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450402 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203746 !Sample_title = cy04.3_CD45pos_S5 !Sample_geo_accession = GSM3203746 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450401 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203747 !Sample_title = cy04.3_CD45pos_S53 !Sample_geo_accession = GSM3203747 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450400 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203748 !Sample_title = cy04.3_CD45pos_S74 !Sample_geo_accession = GSM3203748 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450399 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203749 !Sample_title = cy121.1_CD45pos_S261 !Sample_geo_accession = GSM3203749 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450398 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203750 !Sample_title = cy04.3_CD45pos_S87 !Sample_geo_accession = GSM3203750 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450397 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203751 !Sample_title = cy04.3_CD45pos_S76 !Sample_geo_accession = GSM3203751 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450396 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203752 !Sample_title = cy121.1_CD45pos_S198 !Sample_geo_accession = GSM3203752 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450395 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203753 !Sample_title = cy121.1_CD45pos_S285 !Sample_geo_accession = GSM3203753 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450394 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203754 !Sample_title = cy121.1_CD45pos_S208 !Sample_geo_accession = GSM3203754 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450393 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203755 !Sample_title = cy04.3_CD45pos_S49 !Sample_geo_accession = GSM3203755 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450392 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203756 !Sample_title = cy04.3_CD45pos_S95 !Sample_geo_accession = GSM3203756 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450391 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203757 !Sample_title = cy04.3_CD45pos_S78 !Sample_geo_accession = GSM3203757 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450390 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203758 !Sample_title = cy121.1_CD45pos_S272 !Sample_geo_accession = GSM3203758 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452365 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203759 !Sample_title = cy04.3_CD45pos_S84 !Sample_geo_accession = GSM3203759 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452364 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203760 !Sample_title = cy121.1_CD45neg_S296 !Sample_geo_accession = GSM3203760 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452425 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203761 !Sample_title = cy04.3_CD45pos_S25 !Sample_geo_accession = GSM3203761 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452424 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203762 !Sample_title = cy04.3_CD45pos_S71 !Sample_geo_accession = GSM3203762 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452423 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203763 !Sample_title = cy121.1_CD45pos_S259 !Sample_geo_accession = GSM3203763 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452422 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203764 !Sample_title = cy121.1_CD45pos_S264 !Sample_geo_accession = GSM3203764 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452421 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203765 !Sample_title = cy04.3_CD45pos_S88 !Sample_geo_accession = GSM3203765 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452420 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203766 !Sample_title = cy04.3_CD45pos_S30 !Sample_geo_accession = GSM3203766 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452419 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203767 !Sample_title = cy121.1_CD45pos_S275 !Sample_geo_accession = GSM3203767 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452418 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203768 !Sample_title = cy121.1_CD45neg_S325 !Sample_geo_accession = GSM3203768 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452417 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203769 !Sample_title = cy04.3_CD45pos_S58 !Sample_geo_accession = GSM3203769 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452416 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203770 !Sample_title = cy04.3_CD45pos_S35 !Sample_geo_accession = GSM3203770 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452415 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203771 !Sample_title = cy04.3_CD45pos_S96 !Sample_geo_accession = GSM3203771 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452414 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203772 !Sample_title = cy04.3_CD45pos_S43 !Sample_geo_accession = GSM3203772 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452413 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203773 !Sample_title = cy121.1_CD45neg_S301 !Sample_geo_accession = GSM3203773 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452412 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203774 !Sample_title = cy121.1_CD45pos_S234 !Sample_geo_accession = GSM3203774 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452411 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203775 !Sample_title = cy04.3_CD45pos_S15 !Sample_geo_accession = GSM3203775 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452408 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203776 !Sample_title = cy121.1_CD45pos_S214 !Sample_geo_accession = GSM3203776 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452407 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203777 !Sample_title = cy121.1_CD45pos_S213 !Sample_geo_accession = GSM3203777 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452410 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203778 !Sample_title = cy04.3_CD45pos_S51 !Sample_geo_accession = GSM3203778 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452409 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203779 !Sample_title = cy121.1_CD45pos_S207 !Sample_geo_accession = GSM3203779 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452406 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203780 !Sample_title = cy04.3_CD45pos_S41 !Sample_geo_accession = GSM3203780 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452405 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203781 !Sample_title = cy121.1_CD45neg_S291 !Sample_geo_accession = GSM3203781 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Endo. !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452404 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203782 !Sample_title = cy04.3_CD45pos_S3 !Sample_geo_accession = GSM3203782 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452403 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203783 !Sample_title = cy04.3_CD45pos_S42 !Sample_geo_accession = GSM3203783 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452402 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203784 !Sample_title = cy121.1_CD45pos_S193 !Sample_geo_accession = GSM3203784 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452401 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203785 !Sample_title = cy04.3_CD45pos_S93 !Sample_geo_accession = GSM3203785 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452400 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203786 !Sample_title = cy04.3_CD45pos_S61 !Sample_geo_accession = GSM3203786 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452399 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203787 !Sample_title = cy04.3_CD45pos_S64 !Sample_geo_accession = GSM3203787 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452456 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203788 !Sample_title = cy04.3_CD45pos_S90 !Sample_geo_accession = GSM3203788 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452395 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203789 !Sample_title = cy04.3_CD45pos_S44 !Sample_geo_accession = GSM3203789 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452394 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203790 !Sample_title = cy04.3_CD45pos_S11 !Sample_geo_accession = GSM3203790 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452393 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203791 !Sample_title = cy04.3_CD45pos_S29 !Sample_geo_accession = GSM3203791 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452392 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203792 !Sample_title = cy04.3_CD45pos_S73 !Sample_geo_accession = GSM3203792 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452391 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203793 !Sample_title = cy04.3_CD45pos_S77 !Sample_geo_accession = GSM3203793 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452390 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203794 !Sample_title = cy04.3_CD45pos_S46 !Sample_geo_accession = GSM3203794 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452389 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203795 !Sample_title = cy04.3_CD45pos_S79 !Sample_geo_accession = GSM3203795 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452388 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203796 !Sample_title = cy04.3_CD45pos_S48 !Sample_geo_accession = GSM3203796 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452387 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203797 !Sample_title = cy121.1_CD45neg_S371 !Sample_geo_accession = GSM3203797 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452386 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203798 !Sample_title = cy121.1_CD45neg_S382 !Sample_geo_accession = GSM3203798 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452385 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203799 !Sample_title = cy121.1_CD45pos_S205 !Sample_geo_accession = GSM3203799 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452384 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203800 !Sample_title = cy04.3_CD45pos_S80 !Sample_geo_accession = GSM3203800 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452383 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203801 !Sample_title = cy04.3_CD45pos_S34 !Sample_geo_accession = GSM3203801 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel04.3 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452382 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203802 !Sample_title = cy121.1_CD45pos_S292 !Sample_geo_accession = GSM3203802 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452381 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203803 !Sample_title = cy121.1_CD45pos_S337 !Sample_geo_accession = GSM3203803 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452380 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203804 !Sample_title = cy128_CD45pos_S174 !Sample_geo_accession = GSM3203804 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452379 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203805 !Sample_title = cy129_CD45negCD90neg_S55 !Sample_geo_accession = GSM3203805 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452378 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203806 !Sample_title = cy128_CD45pos_S185 !Sample_geo_accession = GSM3203806 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452377 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203807 !Sample_title = cy128_CD45pos_S156 !Sample_geo_accession = GSM3203807 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452376 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203808 !Sample_title = cy121.1_CD45pos_S300 !Sample_geo_accession = GSM3203808 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452375 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203809 !Sample_title = cy129_CD45negCD90neg_S2 !Sample_geo_accession = GSM3203809 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452374 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203810 !Sample_title = cy121.1_CD45pos_S374 !Sample_geo_accession = GSM3203810 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452373 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203811 !Sample_title = cy129_CD45negCD90neg_S6 !Sample_geo_accession = GSM3203811 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452372 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203812 !Sample_title = cy128_CD45pos_S122 !Sample_geo_accession = GSM3203812 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452371 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203813 !Sample_title = cy129_CD45negCD90neg_S65 !Sample_geo_accession = GSM3203813 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452370 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203814 !Sample_title = cy121.1_CD45pos_S333 !Sample_geo_accession = GSM3203814 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452369 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203815 !Sample_title = cy129_CD45negCD90neg_S91 !Sample_geo_accession = GSM3203815 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452368 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203816 !Sample_title = cy128_CD45neg_S250 !Sample_geo_accession = GSM3203816 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452367 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203817 !Sample_title = cy121.1_CD45pos_S312 !Sample_geo_accession = GSM3203817 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452366 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203818 !Sample_title = cy128_CD45pos_S136 !Sample_geo_accession = GSM3203818 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452360 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203819 !Sample_title = cy121.1_CD45pos_S366 !Sample_geo_accession = GSM3203819 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452359 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203820 !Sample_title = cy128_CD45pos_S152 !Sample_geo_accession = GSM3203820 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452358 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203821 !Sample_title = cy121.1_CD45pos_S360 !Sample_geo_accession = GSM3203821 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452357 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203822 !Sample_title = cy121.1_CD45pos_S355 !Sample_geo_accession = GSM3203822 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452356 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203823 !Sample_title = cy128_CD45pos_S130 !Sample_geo_accession = GSM3203823 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452355 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203824 !Sample_title = cy129_CD45negCD90neg_S25 !Sample_geo_accession = GSM3203824 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452354 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203825 !Sample_title = cy121.1_CD45pos_S341 !Sample_geo_accession = GSM3203825 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452353 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203826 !Sample_title = cy128_CD45pos_S112 !Sample_geo_accession = GSM3203826 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452352 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203827 !Sample_title = cy128_CD45neg_S219 !Sample_geo_accession = GSM3203827 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452351 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203828 !Sample_title = cy121.1_CD45pos_S303 !Sample_geo_accession = GSM3203828 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452350 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203829 !Sample_title = cy128_CD45neg_S255 !Sample_geo_accession = GSM3203829 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452349 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203830 !Sample_title = cy129_CD45negCD90neg_S43 !Sample_geo_accession = GSM3203830 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452348 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203831 !Sample_title = cy128_CD45pos_S133 !Sample_geo_accession = GSM3203831 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452347 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203832 !Sample_title = cy128_CD45pos_S101 !Sample_geo_accession = GSM3203832 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452346 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203833 !Sample_title = cy128_CD45pos_S165 !Sample_geo_accession = GSM3203833 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452344 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203834 !Sample_title = cy121.1_CD45pos_S299 !Sample_geo_accession = GSM3203834 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452343 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203835 !Sample_title = cy128_CD45pos_S97 !Sample_geo_accession = GSM3203835 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452342 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203836 !Sample_title = cy121.1_CD45pos_S290 !Sample_geo_accession = GSM3203836 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452341 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203837 !Sample_title = cy121.1_CD45pos_S362 !Sample_geo_accession = GSM3203837 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452345 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203838 !Sample_title = cy121.1_CD45pos_S327 !Sample_geo_accession = GSM3203838 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452340 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203839 !Sample_title = cy129_CD45negCD90neg_S22 !Sample_geo_accession = GSM3203839 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452339 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203840 !Sample_title = cy121.1_CD45pos_S324 !Sample_geo_accession = GSM3203840 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452338 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203841 !Sample_title = cy129_CD45negCD90neg_S72 !Sample_geo_accession = GSM3203841 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452337 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203842 !Sample_title = cy128_CD45pos_S116 !Sample_geo_accession = GSM3203842 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452336 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203843 !Sample_title = cy128_CD45pos_S141 !Sample_geo_accession = GSM3203843 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452335 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203844 !Sample_title = cy129_CD45negCD90neg_S79 !Sample_geo_accession = GSM3203844 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452334 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203845 !Sample_title = cy129_CD45negCD90neg_S7 !Sample_geo_accession = GSM3203845 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452398 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203846 !Sample_title = cy128_CD45pos_S109 !Sample_geo_accession = GSM3203846 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452397 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203847 !Sample_title = cy128_CD45pos_S159 !Sample_geo_accession = GSM3203847 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452396 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203848 !Sample_title = cy128_CD45neg_S209 !Sample_geo_accession = GSM3203848 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452324 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203849 !Sample_title = cy121.1_CD45pos_S301 !Sample_geo_accession = GSM3203849 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452323 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203850 !Sample_title = cy121.1_CD45pos_S294 !Sample_geo_accession = GSM3203850 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452322 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203851 !Sample_title = cy128_CD45neg_S194 !Sample_geo_accession = GSM3203851 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452321 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203852 !Sample_title = cy128_CD45pos_S180 !Sample_geo_accession = GSM3203852 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452320 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203853 !Sample_title = cy128_CD45pos_S187 !Sample_geo_accession = GSM3203853 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452319 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203854 !Sample_title = cy121.1_CD45pos_S381 !Sample_geo_accession = GSM3203854 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452318 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203855 !Sample_title = cy121.1_CD45pos_S309 !Sample_geo_accession = GSM3203855 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452317 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203856 !Sample_title = cy128_CD45pos_S186 !Sample_geo_accession = GSM3203856 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452316 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203857 !Sample_title = cy129_CD45negCD90neg_S3 !Sample_geo_accession = GSM3203857 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452315 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203858 !Sample_title = cy121.1_CD45pos_S336 !Sample_geo_accession = GSM3203858 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452314 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203859 !Sample_title = cy128_CD45pos_S144 !Sample_geo_accession = GSM3203859 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452313 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203860 !Sample_title = cy128_CD45neg_S231 !Sample_geo_accession = GSM3203860 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452312 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203861 !Sample_title = cy128_CD45pos_S150 !Sample_geo_accession = GSM3203861 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452311 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203862 !Sample_title = cy128_CD45neg_S275 !Sample_geo_accession = GSM3203862 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452310 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203863 !Sample_title = cy128_CD45pos_S173 !Sample_geo_accession = GSM3203863 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452309 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203864 !Sample_title = cy128_CD45neg_S216 !Sample_geo_accession = GSM3203864 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452308 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203865 !Sample_title = cy129_CD45negCD90neg_S70 !Sample_geo_accession = GSM3203865 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452307 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203866 !Sample_title = cy121.1_CD45pos_S293 !Sample_geo_accession = GSM3203866 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452306 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203867 !Sample_title = cy128_CD45pos_S179 !Sample_geo_accession = GSM3203867 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452305 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203868 !Sample_title = cy128_CD45pos_S125 !Sample_geo_accession = GSM3203868 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452304 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203869 !Sample_title = cy129_CD45negCD90neg_S60 !Sample_geo_accession = GSM3203869 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452303 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203870 !Sample_title = cy121.1_CD45pos_S289 !Sample_geo_accession = GSM3203870 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452302 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203871 !Sample_title = cy121.1_CD45pos_S297 !Sample_geo_accession = GSM3203871 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452301 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203872 !Sample_title = cy128_CD45neg_S256 !Sample_geo_accession = GSM3203872 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452300 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203873 !Sample_title = cy129_CD45negCD90neg_S26 !Sample_geo_accession = GSM3203873 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452363 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203874 !Sample_title = cy128_CD45pos_S190 !Sample_geo_accession = GSM3203874 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452362 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203875 !Sample_title = cy121.1_CD45pos_S334 !Sample_geo_accession = GSM3203875 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452361 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203876 !Sample_title = cy128_CD45pos_S151 !Sample_geo_accession = GSM3203876 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452495 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203877 !Sample_title = cy129_CD45negCD90neg_S85 !Sample_geo_accession = GSM3203877 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452494 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203878 !Sample_title = cy121.1_CD45pos_S376 !Sample_geo_accession = GSM3203878 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452493 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203879 !Sample_title = cy129_CD45negCD90neg_S87 !Sample_geo_accession = GSM3203879 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452492 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203880 !Sample_title = cy121.1_CD45pos_S351 !Sample_geo_accession = GSM3203880 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452491 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203881 !Sample_title = cy128_CD45pos_S155 !Sample_geo_accession = GSM3203881 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452490 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203882 !Sample_title = cy129_CD45negCD90neg_S32 !Sample_geo_accession = GSM3203882 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452489 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203883 !Sample_title = cy128_CD45neg_S283 !Sample_geo_accession = GSM3203883 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452488 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203884 !Sample_title = cy128_CD45neg_S196 !Sample_geo_accession = GSM3203884 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452487 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203885 !Sample_title = cy128_CD45neg_S213 !Sample_geo_accession = GSM3203885 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452486 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203886 !Sample_title = cy121.1_CD45pos_S325 !Sample_geo_accession = GSM3203886 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452485 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203887 !Sample_title = cy129_CD45negCD90neg_S46 !Sample_geo_accession = GSM3203887 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452484 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203888 !Sample_title = cy129_CD45negCD90neg_S1 !Sample_geo_accession = GSM3203888 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452483 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203889 !Sample_title = cy128_CD45neg_S281 !Sample_geo_accession = GSM3203889 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452482 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203890 !Sample_title = cy128_CD45pos_S127 !Sample_geo_accession = GSM3203890 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452481 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203891 !Sample_title = cy128_CD45pos_S143 !Sample_geo_accession = GSM3203891 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452480 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203892 !Sample_title = cy128_CD45pos_S182 !Sample_geo_accession = GSM3203892 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452478 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203893 !Sample_title = cy129_CD45negCD90neg_S8 !Sample_geo_accession = GSM3203893 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452477 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203894 !Sample_title = cy121.1_CD45pos_S348 !Sample_geo_accession = GSM3203894 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452476 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203895 !Sample_title = cy129_CD45negCD90neg_S78 !Sample_geo_accession = GSM3203895 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452475 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203896 !Sample_title = cy128_CD45pos_S142 !Sample_geo_accession = GSM3203896 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452474 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203897 !Sample_title = cy121.1_CD45pos_S307 !Sample_geo_accession = GSM3203897 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452479 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203898 !Sample_title = cy121.1_CD45pos_S311 !Sample_geo_accession = GSM3203898 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452473 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203899 !Sample_title = cy121.1_CD45pos_S379 !Sample_geo_accession = GSM3203899 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452472 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203900 !Sample_title = cy128_CD45neg_S261 !Sample_geo_accession = GSM3203900 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452471 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203901 !Sample_title = cy128_CD45neg_S193 !Sample_geo_accession = GSM3203901 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452470 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203902 !Sample_title = cy121.1_CD45pos_S372 !Sample_geo_accession = GSM3203902 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452469 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203903 !Sample_title = cy121.1_CD45pos_S339 !Sample_geo_accession = GSM3203903 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452468 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203904 !Sample_title = cy129_CD45negCD90neg_S56 !Sample_geo_accession = GSM3203904 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452467 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203905 !Sample_title = cy129_CD45negCD90neg_S54 !Sample_geo_accession = GSM3203905 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452466 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203906 !Sample_title = cy121.1_CD45pos_S354 !Sample_geo_accession = GSM3203906 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452465 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203907 !Sample_title = cy121.1_CD45pos_S343 !Sample_geo_accession = GSM3203907 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452464 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203908 !Sample_title = cy121.1_CD45pos_S323 !Sample_geo_accession = GSM3203908 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452463 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203909 !Sample_title = cy129_CD45negCD90neg_S86 !Sample_geo_accession = GSM3203909 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452462 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203910 !Sample_title = cy121.1_CD45pos_S382 !Sample_geo_accession = GSM3203910 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452461 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203911 !Sample_title = cy128_CD45neg_S233 !Sample_geo_accession = GSM3203911 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452460 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203912 !Sample_title = cy121.1_CD45pos_S335 !Sample_geo_accession = GSM3203912 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452459 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203913 !Sample_title = cy129_CD45negCD90neg_S27 !Sample_geo_accession = GSM3203913 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452458 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203914 !Sample_title = cy128_CD45neg_S277 !Sample_geo_accession = GSM3203914 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452457 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203915 !Sample_title = cy129_CD45negCD90neg_S4 !Sample_geo_accession = GSM3203915 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452532 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203916 !Sample_title = cy121.1_CD45pos_S295 !Sample_geo_accession = GSM3203916 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452531 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203917 !Sample_title = cy129_CD45negCD90neg_S44 !Sample_geo_accession = GSM3203917 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452530 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203918 !Sample_title = cy128_CD45neg_S265 !Sample_geo_accession = GSM3203918 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452529 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203919 !Sample_title = cy129_CD45negCD90neg_S68 !Sample_geo_accession = GSM3203919 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452528 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203920 !Sample_title = cy121.1_CD45pos_S370 !Sample_geo_accession = GSM3203920 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452527 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203921 !Sample_title = cy121.1_CD45pos_S330 !Sample_geo_accession = GSM3203921 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452526 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203922 !Sample_title = cy129_CD45negCD90neg_S75 !Sample_geo_accession = GSM3203922 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452525 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203923 !Sample_title = cy121.1_CD45pos_S345 !Sample_geo_accession = GSM3203923 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452524 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203924 !Sample_title = cy121.1_CD45pos_S320 !Sample_geo_accession = GSM3203924 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452523 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203925 !Sample_title = cy129_CD45negCD90neg_S18 !Sample_geo_accession = GSM3203925 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452522 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203926 !Sample_title = cy128_CD45neg_S200 !Sample_geo_accession = GSM3203926 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452521 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203927 !Sample_title = cy121.1_CD45pos_S373 !Sample_geo_accession = GSM3203927 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452520 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203928 !Sample_title = cy121.1_CD45pos_S310 !Sample_geo_accession = GSM3203928 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452519 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203929 !Sample_title = cy128_CD45pos_S160 !Sample_geo_accession = GSM3203929 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452518 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203930 !Sample_title = cy129_CD45negCD90neg_S28 !Sample_geo_accession = GSM3203930 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452517 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203931 !Sample_title = cy128_CD45pos_S161 !Sample_geo_accession = GSM3203931 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452516 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203932 !Sample_title = cy121.1_CD45pos_S350 !Sample_geo_accession = GSM3203932 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452515 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203933 !Sample_title = cy121.1_CD45pos_S346 !Sample_geo_accession = GSM3203933 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452510 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203934 !Sample_title = cy128_CD45pos_S138 !Sample_geo_accession = GSM3203934 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452514 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203935 !Sample_title = cy128_CD45neg_S229 !Sample_geo_accession = GSM3203935 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452513 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203936 !Sample_title = cy128_CD45neg_S212 !Sample_geo_accession = GSM3203936 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452512 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203937 !Sample_title = cy128_CD45pos_S172 !Sample_geo_accession = GSM3203937 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452511 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203938 !Sample_title = cy128_CD45pos_S134 !Sample_geo_accession = GSM3203938 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452509 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203939 !Sample_title = cy128_CD45neg_S232 !Sample_geo_accession = GSM3203939 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452508 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203940 !Sample_title = cy121.1_CD45pos_S364 !Sample_geo_accession = GSM3203940 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452507 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203941 !Sample_title = cy129_CD45negCD90neg_S41 !Sample_geo_accession = GSM3203941 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452506 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203942 !Sample_title = cy129_CD45negCD90neg_S53 !Sample_geo_accession = GSM3203942 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452505 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203943 !Sample_title = cy128_CD45pos_S181 !Sample_geo_accession = GSM3203943 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452504 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203944 !Sample_title = cy128_CD45pos_S178 !Sample_geo_accession = GSM3203944 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452503 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203945 !Sample_title = cy129_CD45negCD90neg_S14 !Sample_geo_accession = GSM3203945 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452502 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203946 !Sample_title = cy121.1_CD45pos_S368 !Sample_geo_accession = GSM3203946 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452501 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203947 !Sample_title = cy121.1_CD45pos_S367 !Sample_geo_accession = GSM3203947 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452500 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203948 !Sample_title = cy129_CD45negCD90neg_S23 !Sample_geo_accession = GSM3203948 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452499 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203949 !Sample_title = cy128_CD45pos_S183 !Sample_geo_accession = GSM3203949 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452498 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203950 !Sample_title = cy121.1_CD45pos_S352 !Sample_geo_accession = GSM3203950 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452497 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203951 !Sample_title = cy128_CD45neg_S248 !Sample_geo_accession = GSM3203951 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452496 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203952 !Sample_title = cy128_CD45pos_S140 !Sample_geo_accession = GSM3203952 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452569 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203953 !Sample_title = cy121.1_CD45pos_S383 !Sample_geo_accession = GSM3203953 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452568 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203954 !Sample_title = cy128_CD45pos_S147 !Sample_geo_accession = GSM3203954 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452567 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203955 !Sample_title = cy129_CD45negCD90neg_S9 !Sample_geo_accession = GSM3203955 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452566 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203956 !Sample_title = cy128_CD45neg_S243 !Sample_geo_accession = GSM3203956 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452565 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203957 !Sample_title = cy129_CD45negCD90neg_S71 !Sample_geo_accession = GSM3203957 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452564 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203958 !Sample_title = cy121.1_CD45pos_S316 !Sample_geo_accession = GSM3203958 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452563 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203959 !Sample_title = cy128_CD45pos_S171 !Sample_geo_accession = GSM3203959 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452562 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203960 !Sample_title = cy121.1_CD45pos_S375 !Sample_geo_accession = GSM3203960 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452561 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203961 !Sample_title = cy129_CD45negCD90neg_S76 !Sample_geo_accession = GSM3203961 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452560 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203962 !Sample_title = cy121.1_CD45pos_S347 !Sample_geo_accession = GSM3203962 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452559 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203963 !Sample_title = cy128_CD45pos_S117 !Sample_geo_accession = GSM3203963 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452558 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203964 !Sample_title = cy128_CD45pos_S114 !Sample_geo_accession = GSM3203964 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452557 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203965 !Sample_title = cy128_CD45pos_S176 !Sample_geo_accession = GSM3203965 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452556 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203966 !Sample_title = cy128_CD45pos_S164 !Sample_geo_accession = GSM3203966 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452455 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203967 !Sample_title = cy121.1_CD45pos_S344 !Sample_geo_accession = GSM3203967 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452454 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203968 !Sample_title = cy128_CD45neg_S253 !Sample_geo_accession = GSM3203968 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452453 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203969 !Sample_title = cy121.1_CD45pos_S313 !Sample_geo_accession = GSM3203969 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452452 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203970 !Sample_title = cy128_CD45pos_S163 !Sample_geo_accession = GSM3203970 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452451 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203971 !Sample_title = cy128_CD45pos_S146 !Sample_geo_accession = GSM3203971 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452450 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203972 !Sample_title = cy128_CD45neg_S287 !Sample_geo_accession = GSM3203972 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452449 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203973 !Sample_title = cy121.1_CD45pos_S322 !Sample_geo_accession = GSM3203973 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452448 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203974 !Sample_title = cy129_CD45negCD90neg_S39 !Sample_geo_accession = GSM3203974 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452447 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203975 !Sample_title = cy121.1_CD45pos_S378 !Sample_geo_accession = GSM3203975 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452442 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203976 !Sample_title = cy121.1_CD45pos_S338 !Sample_geo_accession = GSM3203976 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452446 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203977 !Sample_title = cy128_CD45pos_S177 !Sample_geo_accession = GSM3203977 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452445 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203978 !Sample_title = cy128_CD45pos_S149 !Sample_geo_accession = GSM3203978 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452444 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203979 !Sample_title = cy128_CD45pos_S145 !Sample_geo_accession = GSM3203979 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452443 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203980 !Sample_title = cy129_CD45negCD90neg_S15 !Sample_geo_accession = GSM3203980 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452441 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203981 !Sample_title = cy121.1_CD45pos_S305 !Sample_geo_accession = GSM3203981 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452440 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203982 !Sample_title = cy129_CD45negCD90neg_S47 !Sample_geo_accession = GSM3203982 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452439 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203983 !Sample_title = cy128_CD45neg_S236 !Sample_geo_accession = GSM3203983 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452438 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203984 !Sample_title = cy128_CD45neg_S221 !Sample_geo_accession = GSM3203984 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452437 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203985 !Sample_title = cy129_CD45negCD90neg_S73 !Sample_geo_accession = GSM3203985 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452436 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203986 !Sample_title = cy128_CD45neg_S220 !Sample_geo_accession = GSM3203986 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452435 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203987 !Sample_title = cy128_CD45pos_S169 !Sample_geo_accession = GSM3203987 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452434 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203988 !Sample_title = cy128_CD45pos_S120 !Sample_geo_accession = GSM3203988 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452433 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203989 !Sample_title = cy128_CD45pos_S157 !Sample_geo_accession = GSM3203989 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452432 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203990 !Sample_title = cy121.1_CD45pos_S331 !Sample_geo_accession = GSM3203990 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452431 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203991 !Sample_title = cy121.1_CD45pos_S329 !Sample_geo_accession = GSM3203991 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452430 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203992 !Sample_title = cy129_CD45negCD90neg_S19 !Sample_geo_accession = GSM3203992 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452429 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203993 !Sample_title = cy128_CD45pos_S131 !Sample_geo_accession = GSM3203993 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452428 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203994 !Sample_title = cy128_CD45pos_S170 !Sample_geo_accession = GSM3203994 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452427 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203995 !Sample_title = cy128_CD45pos_S132 !Sample_geo_accession = GSM3203995 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452426 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203996 !Sample_title = cy121.1_CD45pos_S353 !Sample_geo_accession = GSM3203996 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452555 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203997 !Sample_title = cy128_CD45pos_S158 !Sample_geo_accession = GSM3203997 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452554 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203998 !Sample_title = cy121.1_CD45pos_S321 !Sample_geo_accession = GSM3203998 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452552 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3203999 !Sample_title = cy129_CD45negCD90neg_S92 !Sample_geo_accession = GSM3203999 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452551 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204000 !Sample_title = cy128_CD45pos_S168 !Sample_geo_accession = GSM3204000 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: B.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452550 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204001 !Sample_title = cy121.1_CD45pos_S369 !Sample_geo_accession = GSM3204001 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452553 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204002 !Sample_title = cy129_CD45negCD90neg_S77 !Sample_geo_accession = GSM3204002 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452549 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204003 !Sample_title = cy121.1_CD45pos_S340 !Sample_geo_accession = GSM3204003 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452548 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204004 !Sample_title = cy129_CD45negCD90neg_S90 !Sample_geo_accession = GSM3204004 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452547 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204005 !Sample_title = cy121.1_CD45pos_S315 !Sample_geo_accession = GSM3204005 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452546 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204006 !Sample_title = cy128_CD45neg_S270 !Sample_geo_accession = GSM3204006 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel128 !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Mal !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452545 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204007 !Sample_title = cy121.1_CD45pos_S328 !Sample_geo_accession = GSM3204007 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel121.1 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452544 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204008 !Sample_title = cy129PA_CD45pos_S111 !Sample_geo_accession = GSM3204008 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452543 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204009 !Sample_title = cy129PA_CD45pos_S188 !Sample_geo_accession = GSM3204009 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452542 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204010 !Sample_title = cy129PA_CD45pos_S140 !Sample_geo_accession = GSM3204010 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452541 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204011 !Sample_title = cy129PA_CD45pos_S114 !Sample_geo_accession = GSM3204011 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452540 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204012 !Sample_title = cy129PA_CD45pos_S148 !Sample_geo_accession = GSM3204012 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452539 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204013 !Sample_title = cy129PA_CD45pos_S145 !Sample_geo_accession = GSM3204013 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel129pa !Sample_characteristics_ch1 = treatment-group: treatment.naive !Sample_characteristics_ch1 = cohort: New !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452538 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204014 !Sample_title = monika_A12_S100_comb_BCD8_3 !Sample_geo_accession = GSM3204014 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452537 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204015 !Sample_title = CY75_1_CD45_CD8_3__S110_comb_BCD8 !Sample_geo_accession = GSM3204015 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452536 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204016 !Sample_title = monika_E12_S137_comb_BCD8_3 !Sample_geo_accession = GSM3204016 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452535 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204017 !Sample_title = CY75_1_CD45_CD8_7__S213_comb_BCD8 !Sample_geo_accession = GSM3204017 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452534 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204018 !Sample_title = CY75_1_CD45_CD8_3__S170_comb_BCD8 !Sample_geo_accession = GSM3204018 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452533 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204019 !Sample_title = CY75_1_CD45_CD8_8__S384_comb_BCD8 !Sample_geo_accession = GSM3204019 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452602 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204020 !Sample_title = CY75_1_CD45_CD8_1__S96_comb_BCD8 !Sample_geo_accession = GSM3204020 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452601 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204021 !Sample_title = CY75_1_CD45_CD8_3__S162_comb_BCD8 !Sample_geo_accession = GSM3204021 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452600 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204022 !Sample_title = CY75_1_CD45_CD8_7__S256_comb_BCD8 !Sample_geo_accession = GSM3204022 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452599 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204023 !Sample_title = CY75_1_CD45_CD8_7__S194_comb_BCD8 !Sample_geo_accession = GSM3204023 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452598 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204024 !Sample_title = CY75_1_CD45_CD8_8__S348_comb_BCD8 !Sample_geo_accession = GSM3204024 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452597 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204025 !Sample_title = monika_D3_S128_comb_BCD8_3 !Sample_geo_accession = GSM3204025 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09452596 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204026 !Sample_title = CY75_1_CD45_CD8_3__S128_comb_BCD8 !Sample_geo_accession = GSM3204026 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450973 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204027 !Sample_title = CY75_1_CD45_CD8_7__S207_comb_BCD8 !Sample_geo_accession = GSM3204027 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450972 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204028 !Sample_title = monika_D4_S129_comb_BCD8_3 !Sample_geo_accession = GSM3204028 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450971 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204029 !Sample_title = CY75_1_CD45_CD8_8__S310_comb_BCD8 !Sample_geo_accession = GSM3204029 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450970 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204030 !Sample_title = CY75_1_CD45_CD8_3__S169_comb_BCD8 !Sample_geo_accession = GSM3204030 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450969 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204031 !Sample_title = CY75_1_CD45_CD8_7__S284_comb_BCD8 !Sample_geo_accession = GSM3204031 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450960 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204032 !Sample_title = CY75_1_CD45_CD8_3__S182_comb_BCD8 !Sample_geo_accession = GSM3204032 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450959 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204033 !Sample_title = CY75_1_CD45_CD8_3__S159_comb_BCD8 !Sample_geo_accession = GSM3204033 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450958 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204034 !Sample_title = CY75_1_CD45_CD8_8__S316_comb_BCD8 !Sample_geo_accession = GSM3204034 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450957 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204035 !Sample_title = CY75_1_CD45_CD8_3__S124_comb_BCD8 !Sample_geo_accession = GSM3204035 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450956 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204036 !Sample_title = CY75_1_CD45_CD8_7__S206_comb_BCD8 !Sample_geo_accession = GSM3204036 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450955 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204037 !Sample_title = CY75_1_CD45_CD8_7__S240_comb_BCD8 !Sample_geo_accession = GSM3204037 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450954 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204038 !Sample_title = CY75_1_CD45_CD8_8__S318_comb_BCD8 !Sample_geo_accession = GSM3204038 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450953 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204039 !Sample_title = CY75_1_CD45_CD8_3__S157_comb_BCD8 !Sample_geo_accession = GSM3204039 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450952 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204040 !Sample_title = CY75_1_CD45_CD8_7__S246_comb_BCD8 !Sample_geo_accession = GSM3204040 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450951 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204041 !Sample_title = CY75_1_CD45_CD8_8__S303_comb_BCD8 !Sample_geo_accession = GSM3204041 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450950 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204042 !Sample_title = CY75_1_CD45_CD8_8__S319_comb_BCD8 !Sample_geo_accession = GSM3204042 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450949 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204043 !Sample_title = CY75_1_CD45_CD8_7__S273_comb_BCD8 !Sample_geo_accession = GSM3204043 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450948 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204044 !Sample_title = monika_D8_S133_comb_BCD8_3 !Sample_geo_accession = GSM3204044 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450947 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204045 !Sample_title = monika_A10_S98_comb_BCD8_3 !Sample_geo_accession = GSM3204045 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450946 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204046 !Sample_title = CY75_1_CD45_CD8_7__S263_comb_BCD8 !Sample_geo_accession = GSM3204046 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450945 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204047 !Sample_title = CY75_1_CD45_CD8_8__S380_comb_BCD8 !Sample_geo_accession = GSM3204047 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450944 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204048 !Sample_title = CY75_1_CD45_CD8_8__S349_comb_BCD8 !Sample_geo_accession = GSM3204048 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450943 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204049 !Sample_title = monika_C9_S125_comb_BCD8_3 !Sample_geo_accession = GSM3204049 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450942 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204050 !Sample_title = CY75_1_CD45_CD8_1__S90_comb_BCD8 !Sample_geo_accession = GSM3204050 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450941 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204051 !Sample_title = CY75_1_CD45_CD8_3__S104_comb_BCD8 !Sample_geo_accession = GSM3204051 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450940 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204052 !Sample_title = CY75_1_CD45_CD8_8__S378_comb_BCD8 !Sample_geo_accession = GSM3204052 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450939 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204053 !Sample_title = CY75_1_CD45_CD8_8__S294_comb_BCD8 !Sample_geo_accession = GSM3204053 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450938 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204054 !Sample_title = monika_C3_S122_comb_BCD8_3 !Sample_geo_accession = GSM3204054 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450937 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204055 !Sample_title = CY75_1_CD45_CD8_8__S304_comb_BCD8 !Sample_geo_accession = GSM3204055 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450936 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204056 !Sample_title = CY75_1_CD45_CD8_3__S185_comb_BCD8 !Sample_geo_accession = GSM3204056 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: NK !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450935 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204057 !Sample_title = monika_A3_S102_comb_BCD8_3 !Sample_geo_accession = GSM3204057 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450934 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204058 !Sample_title = CY75_1_CD45_CD8_8__S327_comb_BCD8 !Sample_geo_accession = GSM3204058 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450933 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204059 !Sample_title = CY75_1_CD45_CD8_3__S116_comb_BCD8 !Sample_geo_accession = GSM3204059 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450932 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204060 !Sample_title = CY75_1_CD45_CD8_3__S161_comb_BCD8 !Sample_geo_accession = GSM3204060 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450931 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204061 !Sample_title = monika_F5_S148_comb_BCD8_3 !Sample_geo_accession = GSM3204061 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450707 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204062 !Sample_title = monika_B2_S111_comb_BCD8_3 !Sample_geo_accession = GSM3204062 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450706 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204063 !Sample_title = CY75_1_CD45_CD8_3__S187_comb_BCD8 !Sample_geo_accession = GSM3204063 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450705 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204064 !Sample_title = CY75_1_CD45_CD8_3__S189_comb_BCD8 !Sample_geo_accession = GSM3204064 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450704 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204065 !Sample_title = CY75_1_CD45_CD8_7__S283_comb_BCD8 !Sample_geo_accession = GSM3204065 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450703 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204066 !Sample_title = CY75_1_CD45_CD8_8__S347_comb_BCD8 !Sample_geo_accession = GSM3204066 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450702 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204067 !Sample_title = CY75_1_CD45_CD8_3__S175_comb_BCD8 !Sample_geo_accession = GSM3204067 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450701 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204068 !Sample_title = CY75_1_CD45_CD8_8__S356_comb_BCD8 !Sample_geo_accession = GSM3204068 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450700 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204069 !Sample_title = CY75_1_CD45_CD8_3__S190_comb_BCD8 !Sample_geo_accession = GSM3204069 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450699 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204070 !Sample_title = CY75_1_CD45_CD8_7__S223_comb_BCD8 !Sample_geo_accession = GSM3204070 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450698 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204071 !Sample_title = CY75_1_CD45_CD8_8__S322_comb_BCD8 !Sample_geo_accession = GSM3204071 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450697 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204072 !Sample_title = CY75_1_CD45_CD8_8__S292_comb_BCD8 !Sample_geo_accession = GSM3204072 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450696 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204073 !Sample_title = CY75_1_CD45_CD8_8__S374_comb_BCD8 !Sample_geo_accession = GSM3204073 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450695 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204074 !Sample_title = CY75_1_CD45_CD8_8__S333_comb_BCD8 !Sample_geo_accession = GSM3204074 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450694 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204075 !Sample_title = CY75_1_CD45_CD8_7__S285_comb_BCD8 !Sample_geo_accession = GSM3204075 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450693 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204076 !Sample_title = CY75_1_CD45_CD8_8__S337_comb_BCD8 !Sample_geo_accession = GSM3204076 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450692 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204077 !Sample_title = CY75_1_CD45_CD8_8__S360_comb_BCD8 !Sample_geo_accession = GSM3204077 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450691 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204078 !Sample_title = CY75_1_CD45_CD8_7__S198_comb_BCD8 !Sample_geo_accession = GSM3204078 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450690 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204079 !Sample_title = CY75_1_CD45_CD8_3__S174_comb_BCD8 !Sample_geo_accession = GSM3204079 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450689 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204080 !Sample_title = monika_E10_S135_comb_BCD8_3 !Sample_geo_accession = GSM3204080 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450688 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204081 !Sample_title = CY75_1_CD45_CD8_8__S370_comb_BCD8 !Sample_geo_accession = GSM3204081 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450687 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204082 !Sample_title = CY75_1_CD45_CD8_7__S196_comb_BCD8 !Sample_geo_accession = GSM3204082 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450686 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204083 !Sample_title = CY75_1_CD45_CD8_7__S199_comb_BCD8 !Sample_geo_accession = GSM3204083 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450685 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204084 !Sample_title = CY75_1_CD45_CD8_8__S320_comb_BCD8 !Sample_geo_accession = GSM3204084 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450684 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204085 !Sample_title = monika_F7_S150_comb_BCD8_3 !Sample_geo_accession = GSM3204085 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450683 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204086 !Sample_title = monika_B8_S116_comb_BCD8_3 !Sample_geo_accession = GSM3204086 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450682 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204087 !Sample_title = CY75_1_CD45_CD8_8__S296_comb_BCD8 !Sample_geo_accession = GSM3204087 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450680 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204088 !Sample_title = CY75_1_CD45_CD8_7__S197_comb_BCD8 !Sample_geo_accession = GSM3204088 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450679 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204089 !Sample_title = CY75_1_CD45_CD8_3__S106_comb_BCD8 !Sample_geo_accession = GSM3204089 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450678 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204090 !Sample_title = CY75_1_CD45_CD8_3__S123_comb_BCD8 !Sample_geo_accession = GSM3204090 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450663 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204091 !Sample_title = CY75_1_CD45_CD8_8__S306_comb_BCD8 !Sample_geo_accession = GSM3204091 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450681 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204092 !Sample_title = CY75_1_CD45_CD8_8__S290_comb_BCD8 !Sample_geo_accession = GSM3204092 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450662 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204093 !Sample_title = monika_B7_S115_comb_BCD8_3 !Sample_geo_accession = GSM3204093 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450660 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204094 !Sample_title = monika_A6_S105_comb_BCD8_3 !Sample_geo_accession = GSM3204094 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450659 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204095 !Sample_title = CY75_1_CD45_CD8_3__S147_comb_BCD8 !Sample_geo_accession = GSM3204095 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450658 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204096 !Sample_title = CY75_1_CD45_CD8_8__S381_comb_BCD8 !Sample_geo_accession = GSM3204096 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450657 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204097 !Sample_title = CY75_1_CD45_CD8_7__S274_comb_BCD8 !Sample_geo_accession = GSM3204097 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450656 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204098 !Sample_title = monika_F1_S144_comb_BCD8_3 !Sample_geo_accession = GSM3204098 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450655 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204099 !Sample_title = monika_B10_S109_comb_BCD8_3 !Sample_geo_accession = GSM3204099 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450654 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204100 !Sample_title = monika_A4_S103_comb_BCD8_3 !Sample_geo_accession = GSM3204100 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450653 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204101 !Sample_title = CY75_1_CD45_CD8_7__S262_comb_BCD8 !Sample_geo_accession = GSM3204101 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450652 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204102 !Sample_title = CY75_1_CD45_CD8_7__S241_comb_BCD8 !Sample_geo_accession = GSM3204102 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450651 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204103 !Sample_title = CY75_1_CD45_CD8_7__S212_comb_BCD8 !Sample_geo_accession = GSM3204103 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450650 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204104 !Sample_title = CY75_1_CD45_CD8_7__S203_comb_BCD8 !Sample_geo_accession = GSM3204104 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450649 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204105 !Sample_title = monika_F9_S152_comb_BCD8_3 !Sample_geo_accession = GSM3204105 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450648 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204106 !Sample_title = CY75_1_CD45_CD8_7__S221_comb_BCD8 !Sample_geo_accession = GSM3204106 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450647 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204107 !Sample_title = CY75_1_CD45_CD8_3__S155_comb_BCD8 !Sample_geo_accession = GSM3204107 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450646 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204108 !Sample_title = CY75_1_CD45_CD8_7__S255_comb_BCD8 !Sample_geo_accession = GSM3204108 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450645 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204109 !Sample_title = CY75_1_CD45_CD8_3__S172_comb_BCD8 !Sample_geo_accession = GSM3204109 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450644 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204110 !Sample_title = CY75_1_CD45_CD8_8__S345_comb_BCD8 !Sample_geo_accession = GSM3204110 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450643 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204111 !Sample_title = CY75_1_CD45_CD8_8__S350_comb_BCD8 !Sample_geo_accession = GSM3204111 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450642 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204112 !Sample_title = CY75_1_CD45_CD8_8__S336_comb_BCD8 !Sample_geo_accession = GSM3204112 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450641 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204113 !Sample_title = CY75_1_CD45_CD8_3__S108_comb_BCD8 !Sample_geo_accession = GSM3204113 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450640 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204114 !Sample_title = CY75_1_CD45_CD8_3__S184_comb_BCD8 !Sample_geo_accession = GSM3204114 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450639 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204115 !Sample_title = CY75_1_CD45_CD8_3__S158_comb_BCD8 !Sample_geo_accession = GSM3204115 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450638 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204116 !Sample_title = CY75_1_CD45_CD8_3__S138_comb_BCD8 !Sample_geo_accession = GSM3204116 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450637 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204117 !Sample_title = CY75_1_CD45_CD8_3__S122_comb_BCD8 !Sample_geo_accession = GSM3204117 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450636 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204118 !Sample_title = CY75_1_CD45_CD8_1__S93_comb_BCD8 !Sample_geo_accession = GSM3204118 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450635 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204119 !Sample_title = monika_F4_S147_comb_BCD8_3 !Sample_geo_accession = GSM3204119 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450709 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204120 !Sample_title = CY75_1_CD45_CD8_7__S282_comb_BCD8 !Sample_geo_accession = GSM3204120 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450708 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204121 !Sample_title = CY75_1_CD45_CD8_8__S323_comb_BCD8 !Sample_geo_accession = GSM3204121 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450618 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204122 !Sample_title = CY75_1_CD45_CD8_3__S117_comb_BCD8 !Sample_geo_accession = GSM3204122 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450617 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204123 !Sample_title = CY75_1_CD45_CD8_7__S249_comb_BCD8 !Sample_geo_accession = GSM3204123 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450616 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204124 !Sample_title = CY75_1_CD45_CD8_7__S259_comb_BCD8 !Sample_geo_accession = GSM3204124 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450615 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204125 !Sample_title = CY75_1_CD45_CD8_7__S269_comb_BCD8 !Sample_geo_accession = GSM3204125 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450614 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204126 !Sample_title = monika_C6_S124_comb_BCD8_3 !Sample_geo_accession = GSM3204126 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450613 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204127 !Sample_title = monika_E8_S142_comb_BCD8_3 !Sample_geo_accession = GSM3204127 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450612 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204128 !Sample_title = monika_B5_S113_comb_BCD8_3 !Sample_geo_accession = GSM3204128 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450611 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204129 !Sample_title = CY75_1_CD45_CD8_8__S326_comb_BCD8 !Sample_geo_accession = GSM3204129 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450610 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204130 !Sample_title = monika_D1_S126_comb_BCD8_3 !Sample_geo_accession = GSM3204130 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450609 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204131 !Sample_title = CY75_1_CD45_CD8_1__S95_comb_BCD8 !Sample_geo_accession = GSM3204131 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450608 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204132 !Sample_title = monika_D6_S131_comb_BCD8_3 !Sample_geo_accession = GSM3204132 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450607 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204133 !Sample_title = CY75_1_CD45_CD8_7__S218_comb_BCD8 !Sample_geo_accession = GSM3204133 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450606 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204134 !Sample_title = CY75_1_CD45_CD8_8__S375_comb_BCD8 !Sample_geo_accession = GSM3204134 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450605 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204135 !Sample_title = CY75_1_CD45_CD8_8__S377_comb_BCD8 !Sample_geo_accession = GSM3204135 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450604 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204136 !Sample_title = CY75_1_CD45_CD8_7__S270_comb_BCD8 !Sample_geo_accession = GSM3204136 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450603 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204137 !Sample_title = CY75_1_CD45_CD8_7__S242_comb_BCD8 !Sample_geo_accession = GSM3204137 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450602 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204138 !Sample_title = monika_F10_S145_comb_BCD8_3 !Sample_geo_accession = GSM3204138 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450601 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204139 !Sample_title = CY75_1_CD45_CD8_8__S368_comb_BCD8 !Sample_geo_accession = GSM3204139 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450600 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204140 !Sample_title = CY75_1_CD45_CD8_8__S352_comb_BCD8 !Sample_geo_accession = GSM3204140 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450599 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204141 !Sample_title = CY75_1_CD45_CD8_7__S238_comb_BCD8 !Sample_geo_accession = GSM3204141 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450598 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204142 !Sample_title = CY75_1_CD45_CD8_7__S215_comb_BCD8 !Sample_geo_accession = GSM3204142 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450672 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204143 !Sample_title = CY75_1_CD45_CD8_8__S344_comb_BCD8 !Sample_geo_accession = GSM3204143 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450671 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204144 !Sample_title = monika_E6_S140_comb_BCD8_3 !Sample_geo_accession = GSM3204144 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450670 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204145 !Sample_title = CY75_1_CD45_CD8_7__S216_comb_BCD8 !Sample_geo_accession = GSM3204145 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450669 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204146 !Sample_title = CY75_1_CD45_CD8_8__S328_comb_BCD8 !Sample_geo_accession = GSM3204146 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450668 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204147 !Sample_title = CY75_1_CD45_CD8_7__S228_comb_BCD8 !Sample_geo_accession = GSM3204147 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450667 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204148 !Sample_title = CY75_1_CD45_CD8_3__S112_comb_BCD8 !Sample_geo_accession = GSM3204148 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450666 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204149 !Sample_title = CY75_1_CD45_CD8_3__S164_comb_BCD8 !Sample_geo_accession = GSM3204149 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450665 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204150 !Sample_title = CY75_1_CD45_CD8_7__S279_comb_BCD8 !Sample_geo_accession = GSM3204150 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450664 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204151 !Sample_title = CY75_1_CD45_CD8_3__S120_comb_BCD8 !Sample_geo_accession = GSM3204151 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450576 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204152 !Sample_title = CY75_1_CD45_CD8_7__S266_comb_BCD8 !Sample_geo_accession = GSM3204152 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450575 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204153 !Sample_title = CY75_1_CD45_CD8_3__S178_comb_BCD8 !Sample_geo_accession = GSM3204153 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450574 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204154 !Sample_title = CY75_1_CD45_CD8_7__S234_comb_BCD8 !Sample_geo_accession = GSM3204154 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450573 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204155 !Sample_title = monika_E11_S136_comb_BCD8_3 !Sample_geo_accession = GSM3204155 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450572 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204156 !Sample_title = CY75_1_CD45_CD8_8__S324_comb_BCD8 !Sample_geo_accession = GSM3204156 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450571 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204157 !Sample_title = CY75_1_CD45_CD8_3__S107_comb_BCD8 !Sample_geo_accession = GSM3204157 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450570 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204158 !Sample_title = monika_A5_S104_comb_BCD8_3 !Sample_geo_accession = GSM3204158 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450569 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204159 !Sample_title = CY75_1_CD45_CD8_7__S258_comb_BCD8 !Sample_geo_accession = GSM3204159 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450568 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204160 !Sample_title = monika_A8_S107_comb_BCD8_3 !Sample_geo_accession = GSM3204160 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450567 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204161 !Sample_title = CY75_1_CD45_CD8_8__S343_comb_BCD8 !Sample_geo_accession = GSM3204161 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450566 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204162 !Sample_title = CY75_1_CD45_CD8_8__S321_comb_BCD8 !Sample_geo_accession = GSM3204162 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450565 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204163 !Sample_title = CY75_1_CD45_CD8_7__S202_comb_BCD8 !Sample_geo_accession = GSM3204163 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450564 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204164 !Sample_title = monika_D11_S127_comb_BCD8_3 !Sample_geo_accession = GSM3204164 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450563 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204165 !Sample_title = CY75_1_CD45_CD8_3__S137_comb_BCD8 !Sample_geo_accession = GSM3204165 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450634 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204166 !Sample_title = CY75_1_CD45_CD8_7__S288_comb_BCD8 !Sample_geo_accession = GSM3204166 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450633 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204167 !Sample_title = CY75_1_CD45_CD8_8__S354_comb_BCD8 !Sample_geo_accession = GSM3204167 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450632 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204168 !Sample_title = CY75_1_CD45_CD8_1__S91_comb_BCD8 !Sample_geo_accession = GSM3204168 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450631 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204169 !Sample_title = CY75_1_CD45_CD8_3__S141_comb_BCD8 !Sample_geo_accession = GSM3204169 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450630 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204170 !Sample_title = CY75_1_CD45_CD8_8__S315_comb_BCD8 !Sample_geo_accession = GSM3204170 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450629 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204171 !Sample_title = CY75_1_CD45_CD8_3__S129_comb_BCD8 !Sample_geo_accession = GSM3204171 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450628 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204172 !Sample_title = monika_B6_S114_comb_BCD8_3 !Sample_geo_accession = GSM3204172 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450627 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204173 !Sample_title = CY75_1_CD45_CD8_7__S225_comb_BCD8 !Sample_geo_accession = GSM3204173 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450626 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204174 !Sample_title = CY75_1_CD45_CD8_3__S180_comb_BCD8 !Sample_geo_accession = GSM3204174 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450625 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204175 !Sample_title = CY75_1_CD45_CD8_7__S253_comb_BCD8 !Sample_geo_accession = GSM3204175 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450624 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204176 !Sample_title = CY75_1_CD45_CD8_3__S97_comb_BCD8 !Sample_geo_accession = GSM3204176 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450623 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204177 !Sample_title = CY75_1_CD45_CD8_8__S379_comb_BCD8 !Sample_geo_accession = GSM3204177 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450622 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204178 !Sample_title = CY75_1_CD45_CD8_8__S383_comb_BCD8 !Sample_geo_accession = GSM3204178 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450621 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204179 !Sample_title = monika_F6_S149_comb_BCD8_3 !Sample_geo_accession = GSM3204179 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450620 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204180 !Sample_title = monika_B11_S110_comb_BCD8_3 !Sample_geo_accession = GSM3204180 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450619 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204181 !Sample_title = CY75_1_CD45_CD8_1__S92_comb_BCD8 !Sample_geo_accession = GSM3204181 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450538 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204182 !Sample_title = CY75_1_CD45_CD8_7__S254_comb_BCD8 !Sample_geo_accession = GSM3204182 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450537 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204183 !Sample_title = CY75_1_CD45_CD8_3__S111_comb_BCD8 !Sample_geo_accession = GSM3204183 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450536 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204184 !Sample_title = monika_A2_S101_comb_BCD8_3 !Sample_geo_accession = GSM3204184 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450535 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204185 !Sample_title = monika_F2_S146_comb_BCD8_3 !Sample_geo_accession = GSM3204185 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450534 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204186 !Sample_title = CY75_1_CD45_CD8_7__S244_comb_BCD8 !Sample_geo_accession = GSM3204186 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450533 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204187 !Sample_title = CY75_1_CD45_CD8_8__S298_comb_BCD8 !Sample_geo_accession = GSM3204187 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450532 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204188 !Sample_title = CY75_1_CD45_CD8_3__S150_comb_BCD8 !Sample_geo_accession = GSM3204188 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450531 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204189 !Sample_title = CY75_1_CD45_CD8_3__S179_comb_BCD8 !Sample_geo_accession = GSM3204189 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450597 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204190 !Sample_title = CY75_1_CD45_CD8_8__S295_comb_BCD8 !Sample_geo_accession = GSM3204190 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450596 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204191 !Sample_title = CY75_1_CD45_CD8_7__S257_comb_BCD8 !Sample_geo_accession = GSM3204191 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450595 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204192 !Sample_title = CY75_1_CD45_CD8_3__S134_comb_BCD8 !Sample_geo_accession = GSM3204192 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450594 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204193 !Sample_title = CY75_1_CD45_CD8_7__S209_comb_BCD8 !Sample_geo_accession = GSM3204193 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450593 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204194 !Sample_title = CY75_1_CD45_CD8_3__S105_comb_BCD8 !Sample_geo_accession = GSM3204194 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450592 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204195 !Sample_title = CY75_1_CD45_CD8_3__S113_comb_BCD8 !Sample_geo_accession = GSM3204195 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450591 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204196 !Sample_title = CY75_1_CD45_CD8_7__S230_comb_BCD8 !Sample_geo_accession = GSM3204196 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450590 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204197 !Sample_title = CY75_1_CD45_CD8_1__S94_comb_BCD8 !Sample_geo_accession = GSM3204197 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450589 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204198 !Sample_title = CY75_1_CD45_CD8_7__S248_comb_BCD8 !Sample_geo_accession = GSM3204198 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450588 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204199 !Sample_title = CY75_1_CD45_CD8_8__S367_comb_BCD8 !Sample_geo_accession = GSM3204199 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450587 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204200 !Sample_title = CY75_1_CD45_CD8_3__S163_comb_BCD8 !Sample_geo_accession = GSM3204200 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450586 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204201 !Sample_title = CY75_1_CD45_CD8_7__S265_comb_BCD8 !Sample_geo_accession = GSM3204201 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450585 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204202 !Sample_title = monika_E5_S139_comb_BCD8_3 !Sample_geo_accession = GSM3204202 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450584 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204203 !Sample_title = CY75_1_CD45_CD8_8__S307_comb_BCD8 !Sample_geo_accession = GSM3204203 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450583 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204204 !Sample_title = CY75_1_CD45_CD8_8__S334_comb_BCD8 !Sample_geo_accession = GSM3204204 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450582 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204205 !Sample_title = CY75_1_CD45_CD8_3__S186_comb_BCD8 !Sample_geo_accession = GSM3204205 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450581 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204206 !Sample_title = CY75_1_CD45_CD8_7__S286_comb_BCD8 !Sample_geo_accession = GSM3204206 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450580 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204207 !Sample_title = monika_C5_S123_comb_BCD8_3 !Sample_geo_accession = GSM3204207 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450579 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204208 !Sample_title = monika_B9_S117_comb_BCD8_3 !Sample_geo_accession = GSM3204208 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450578 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204209 !Sample_title = CY75_1_CD45_CD8_3__S154_comb_BCD8 !Sample_geo_accession = GSM3204209 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09450577 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204210 !Sample_title = CY75_1_CD45_CD8_8__S299_comb_BCD8 !Sample_geo_accession = GSM3204210 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451315 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204211 !Sample_title = CY75_1_CD45_CD8_3__S133_comb_BCD8 !Sample_geo_accession = GSM3204211 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451322 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204212 !Sample_title = CY75_1_CD45_CD8_3__S119_comb_BCD8 !Sample_geo_accession = GSM3204212 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451321 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204213 !Sample_title = CY75_1_CD45_CD8_3__S121_comb_BCD8 !Sample_geo_accession = GSM3204213 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451320 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204214 !Sample_title = CY75_1_CD45_CD8_7__S211_comb_BCD8 !Sample_geo_accession = GSM3204214 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451319 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204215 !Sample_title = CY75_1_CD45_CD8_8__S339_comb_BCD8 !Sample_geo_accession = GSM3204215 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451318 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204216 !Sample_title = CY75_1_CD45_CD8_7__S200_comb_BCD8 !Sample_geo_accession = GSM3204216 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451317 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204217 !Sample_title = CY75_1_CD45_CD8_3__S173_comb_BCD8 !Sample_geo_accession = GSM3204217 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451316 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204218 !Sample_title = CY75_1_CD45_CD8_3__S156_comb_BCD8 !Sample_geo_accession = GSM3204218 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451314 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204219 !Sample_title = CY75_1_CD45_CD8_8__S376_comb_BCD8 !Sample_geo_accession = GSM3204219 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451313 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204220 !Sample_title = CY75_1_CD45_CD8_8__S353_comb_BCD8 !Sample_geo_accession = GSM3204220 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451312 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204221 !Sample_title = CY75_1_CD45_CD8_8__S291_comb_BCD8 !Sample_geo_accession = GSM3204221 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451311 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204222 !Sample_title = CY75_1_CD45_CD8_3__S139_comb_BCD8 !Sample_geo_accession = GSM3204222 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451310 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204223 !Sample_title = monika_E9_S143_comb_BCD8_3 !Sample_geo_accession = GSM3204223 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451309 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204224 !Sample_title = CY75_1_CD45_CD8_7__S252_comb_BCD8 !Sample_geo_accession = GSM3204224 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451308 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204225 !Sample_title = monika_E2_S138_comb_BCD8_3 !Sample_geo_accession = GSM3204225 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451307 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204226 !Sample_title = CY75_1_CD45_CD8_3__S100_comb_BCD8 !Sample_geo_accession = GSM3204226 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451306 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204227 !Sample_title = CY75_1_CD45_CD8_7__S220_comb_BCD8 !Sample_geo_accession = GSM3204227 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451305 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204228 !Sample_title = monika_A7_S106_comb_BCD8_3 !Sample_geo_accession = GSM3204228 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451304 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204229 !Sample_title = CY75_1_CD45_CD8_7__S250_comb_BCD8 !Sample_geo_accession = GSM3204229 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451303 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204230 !Sample_title = CY75_1_CD45_CD8_3__S166_comb_BCD8 !Sample_geo_accession = GSM3204230 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451302 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204231 !Sample_title = CY75_1_CD45_CD8_3__S114_comb_BCD8 !Sample_geo_accession = GSM3204231 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451301 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204232 !Sample_title = CY75_1_CD45_CD8_8__S371_comb_BCD8 !Sample_geo_accession = GSM3204232 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451300 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204233 !Sample_title = CY75_1_CD45_CD8_8__S357_comb_BCD8 !Sample_geo_accession = GSM3204233 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451299 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204234 !Sample_title = CY75_1_CD45_CD8_8__S300_comb_BCD8 !Sample_geo_accession = GSM3204234 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451298 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204235 !Sample_title = CY75_1_CD45_CD8_3__S135_comb_BCD8 !Sample_geo_accession = GSM3204235 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451297 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204236 !Sample_title = CY75_1_CD45_CD8_7__S224_comb_BCD8 !Sample_geo_accession = GSM3204236 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451296 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204237 !Sample_title = CY75_1_CD45_CD8_3__S132_comb_BCD8 !Sample_geo_accession = GSM3204237 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451295 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204238 !Sample_title = CY75_1_CD45_CD8_3__S160_comb_BCD8 !Sample_geo_accession = GSM3204238 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451294 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204239 !Sample_title = CY75_1_CD45_CD8_7__S260_comb_BCD8 !Sample_geo_accession = GSM3204239 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451293 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204240 !Sample_title = CY75_1_CD45_CD8_7__S268_comb_BCD8 !Sample_geo_accession = GSM3204240 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451473 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204241 !Sample_title = CY75_1_CD45_CD8_3__S99_comb_BCD8 !Sample_geo_accession = GSM3204241 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451472 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204242 !Sample_title = monika_E1_S134_comb_BCD8_3 !Sample_geo_accession = GSM3204242 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451471 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204243 !Sample_title = CY75_1_CD45_CD8_8__S362_comb_BCD8 !Sample_geo_accession = GSM3204243 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451470 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204244 !Sample_title = CY75_1_CD45_CD8_7__S278_comb_BCD8 !Sample_geo_accession = GSM3204244 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451469 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204245 !Sample_title = CY75_1_CD45_CD8_7__S231_comb_BCD8 !Sample_geo_accession = GSM3204245 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451468 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204246 !Sample_title = monika_A11_S99_comb_BCD8_3 !Sample_geo_accession = GSM3204246 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451467 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204247 !Sample_title = monika_B3_S112_comb_BCD8_3 !Sample_geo_accession = GSM3204247 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451466 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204248 !Sample_title = monika_D5_S130_comb_BCD8_3 !Sample_geo_accession = GSM3204248 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451465 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204249 !Sample_title = CY75_1_CD45_CD8_7__S264_comb_BCD8 !Sample_geo_accession = GSM3204249 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451464 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204250 !Sample_title = CY75_1_CD45_CD8_8__S309_comb_BCD8 !Sample_geo_accession = GSM3204250 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451463 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204251 !Sample_title = CY75_1_CD45_CD8_8__S382_comb_BCD8 !Sample_geo_accession = GSM3204251 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451462 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204252 !Sample_title = CY75_1_CD45_CD8_8__S293_comb_BCD8 !Sample_geo_accession = GSM3204252 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451461 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204253 !Sample_title = CY75_1_CD45_CD8_3__S149_comb_BCD8 !Sample_geo_accession = GSM3204253 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451487 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204254 !Sample_title = CY75_1_CD45_CD8_8__S355_comb_BCD8 !Sample_geo_accession = GSM3204254 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451486 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204255 !Sample_title = CY75_1_CD45_CD8_3__S148_comb_BCD8 !Sample_geo_accession = GSM3204255 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451485 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204256 !Sample_title = monika_F8_S151_comb_BCD8_3 !Sample_geo_accession = GSM3204256 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451484 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204257 !Sample_title = CY75_1_CD45_CD8_8__S364_comb_BCD8 !Sample_geo_accession = GSM3204257 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451483 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204258 !Sample_title = CY75_1_CD45_CD8_3__S171_comb_BCD8 !Sample_geo_accession = GSM3204258 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451482 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204259 !Sample_title = CY75_1_CD45_CD8_7__S247_comb_BCD8 !Sample_geo_accession = GSM3204259 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451481 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204260 !Sample_title = CY75_1_CD45_CD8_3__S127_comb_BCD8 !Sample_geo_accession = GSM3204260 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451480 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204261 !Sample_title = CY75_1_CD45_CD8_7__S236_comb_BCD8 !Sample_geo_accession = GSM3204261 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451479 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204262 !Sample_title = CY75_1_CD45_CD8_8__S335_comb_BCD8 !Sample_geo_accession = GSM3204262 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451478 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204263 !Sample_title = CY75_1_CD45_CD8_7__S229_comb_BCD8 !Sample_geo_accession = GSM3204263 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451544 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204264 !Sample_title = CY75_1_CD45_CD8_7__S227_comb_BCD8 !Sample_geo_accession = GSM3204264 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Macrophage !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451543 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204265 !Sample_title = CY75_1_CD45_CD8_7__S222_comb_BCD8 !Sample_geo_accession = GSM3204265 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: Undetermined !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451542 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204266 !Sample_title = CY75_1_CD45_CD8_8__S313_comb_BCD8 !Sample_geo_accession = GSM3204266 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451541 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204267 !Sample_title = monika_C12_S120_comb_BCD8_3 !Sample_geo_accession = GSM3204267 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451540 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204268 !Sample_title = CY75_1_CD45_CD8_7__S235_comb_BCD8 !Sample_geo_accession = GSM3204268 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451539 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204269 !Sample_title = monika_B1_S108_comb_BCD8_3 !Sample_geo_accession = GSM3204269 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451538 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204270 !Sample_title = CY75_1_CD45_CD8_3__S126_comb_BCD8 !Sample_geo_accession = GSM3204270 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451537 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204271 !Sample_title = CY75_1_CD45_CD8_7__S226_comb_BCD8 !Sample_geo_accession = GSM3204271 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451536 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204272 !Sample_title = monika_A1_S97_comb_BCD8_3 !Sample_geo_accession = GSM3204272 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451535 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204273 !Sample_title = CY75_1_CD45_CD8_7__S205_comb_BCD8 !Sample_geo_accession = GSM3204273 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451534 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204274 !Sample_title = CY75_1_CD45_CD8_7__S245_comb_BCD8 !Sample_geo_accession = GSM3204274 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451533 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204275 !Sample_title = CY75_1_CD45_CD8_8__S359_comb_BCD8 !Sample_geo_accession = GSM3204275 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451532 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204276 !Sample_title = CY75_1_CD45_CD8_8__S363_comb_BCD8 !Sample_geo_accession = GSM3204276 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451531 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204277 !Sample_title = CY75_1_CD45_CD8_8__S341_comb_BCD8 !Sample_geo_accession = GSM3204277 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451530 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204278 !Sample_title = CY75_1_CD45_CD8_8__S365_comb_BCD8 !Sample_geo_accession = GSM3204278 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451529 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204279 !Sample_title = CY75_1_CD45_CD8_7__S208_comb_BCD8 !Sample_geo_accession = GSM3204279 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451528 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204280 !Sample_title = CY75_1_CD45_CD8_8__S312_comb_BCD8 !Sample_geo_accession = GSM3204280 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451527 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204281 !Sample_title = CY75_1_CD45_CD8_8__S372_comb_BCD8 !Sample_geo_accession = GSM3204281 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451526 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204282 !Sample_title = CY75_1_CD45_CD8_3__S183_comb_BCD8 !Sample_geo_accession = GSM3204282 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451525 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204283 !Sample_title = CY75_1_CD45_CD8_8__S361_comb_BCD8 !Sample_geo_accession = GSM3204283 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451460 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204284 !Sample_title = CY75_1_CD45_CD8_8__S342_comb_BCD8 !Sample_geo_accession = GSM3204284 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451459 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204285 !Sample_title = CY75_1_CD45_CD8_7__S271_comb_BCD8 !Sample_geo_accession = GSM3204285 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451458 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204286 !Sample_title = CY75_1_CD45_CD8_7__S217_comb_BCD8 !Sample_geo_accession = GSM3204286 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451457 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204287 !Sample_title = CY75_1_CD45_CD8_7__S214_comb_BCD8 !Sample_geo_accession = GSM3204287 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451456 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204288 !Sample_title = CY75_1_CD45_CD8_7__S287_comb_BCD8 !Sample_geo_accession = GSM3204288 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451455 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204289 !Sample_title = CY75_1_CD45_CD8_3__S181_comb_BCD8 !Sample_geo_accession = GSM3204289 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451454 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204290 !Sample_title = CY75_1_CD45_CD8_8__S302_comb_BCD8 !Sample_geo_accession = GSM3204290 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.cell !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451453 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204291 !Sample_title = monika_C11_S119_comb_BCD8_3 !Sample_geo_accession = GSM3204291 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD4 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451452 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204292 !Sample_title = CY75_1_CD45_CD8_8__S331_comb_BCD8 !Sample_geo_accession = GSM3204292 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451451 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204293 !Sample_title = CY75_1_CD45_CD8_7__S210_comb_BCD8 !Sample_geo_accession = GSM3204293 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451450 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204294 !Sample_title = CY75_1_CD45_CD8_3__S142_comb_BCD8 !Sample_geo_accession = GSM3204294 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451449 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204295 !Sample_title = CY75_1_CD45_CD8_7__S280_comb_BCD8 !Sample_geo_accession = GSM3204295 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451448 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204296 !Sample_title = CY75_1_CD45_CD8_3__S168_comb_BCD8 !Sample_geo_accession = GSM3204296 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451447 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204297 !Sample_title = CY75_1_CD45_CD8_8__S338_comb_BCD8 !Sample_geo_accession = GSM3204297 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451446 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204298 !Sample_title = monika_D7_S132_comb_BCD8_3 !Sample_geo_accession = GSM3204298 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451445 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204299 !Sample_title = CY75_1_CD45_CD8_8__S289_comb_BCD8 !Sample_geo_accession = GSM3204299 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451444 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0 ^SAMPLE = GSM3204300 !Sample_title = CY75_1_CD45_CD8_8__S351_comb_BCD8 !Sample_geo_accession = GSM3204300 !Sample_status = Public on Nov 01 2018 !Sample_submission_date = Jun 18 2018 !Sample_last_update_date = Nov 01 2018 !Sample_type = SRA !Sample_channel_count = 1 !Sample_source_name_ch1 = melanoma tumor !Sample_organism_ch1 = Homo sapiens !Sample_taxid_ch1 = 9606 !Sample_characteristics_ch1 = tumor-of-origin: Mel75 !Sample_characteristics_ch1 = treatment-group: post.treatment !Sample_characteristics_ch1 = cohort: Tirosh !Sample_characteristics_ch1 = cell type: T.CD8 !Sample_molecule_ch1 = total RNA !Sample_extract_protocol_ch1 = Dissociated individual cells from fresh tumor resections. Isolated immune and non-immune cells by FACS based on CD45 staining. !Sample_extract_protocol_ch1 = Full-length RNAseq (SMART-Seq2) libraries sequenced with 38bp paired-end reads on an Illumina NextSeq 500. !Sample_description = Full-length RNAseq (SMART-Seq2) reads. !Sample_description = processed data file: counts.csv !Sample_description = processed data file: tpm.csv !Sample_description = processed data file: cell.annotations.csv !Sample_data_processing = Full-length RNAseq (SMART-Seq2). !Sample_data_processing = Tophat !Sample_data_processing = Bowtie mapping to UCSC hg19 human transcriptome. !Sample_data_processing = RSEM v1.2.8 !Sample_data_processing = Genome_build: hg19 (GRCh37) !Sample_data_processing = Supplementary_files_format_and_content: cell.annotations.csv: Comma-separated cell sample annotation table; For each cell (row): cell name, sample type, cell type, treatment group, cohort, number of genes, number of reads. !Sample_data_processing = Supplementary_files_format_and_content: counts.csv: Comma-separated counts table; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in counts as calculated using RSEM. !Sample_data_processing = Supplementary_files_format_and_content: tpm.csv: Comma-separated log-transformed transcripts per million (TPM) table; Values are provided as log2[(TPM/10) +1]; Full-length RNAseq (SMART-Seq2); The expression levels for each gene (rows) in each single cell (column) in transcripts per million (TPM) as calculated using RSEM. !Sample_platform_id = GPL18573 !Sample_contact_name = Orr,,Ashenberg !Sample_contact_laboratory = Aviv Regev !Sample_contact_department = Klarman Cell Observatory !Sample_contact_institute = Broad Institute !Sample_contact_address = 415 Main Street !Sample_contact_city = Cambridge !Sample_contact_state = MA !Sample_contact_zip/postal_code = 02142 !Sample_contact_country = USA !Sample_instrument_model = Illumina NextSeq 500 !Sample_library_selection = cDNA !Sample_library_source = transcriptomic !Sample_library_strategy = RNA-Seq !Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN09451443 !Sample_supplementary_file_1 = NONE !Sample_series_id = GSE115978 !Sample_data_row_count = 0